A long-term cryopreservation device for biological germplasm resources

Abstract

This invention provides a long-term cryopreservation device for biological germplasm resources, including a heat-insulated storage rack with multiple storage slots on the front side of the rack. A sealed storage container with a front opening is detachably connected to each storage slot. A sealing cap is detachably connected to the opening of the sealed storage container. A cap groove that mates with the sealing cap is provided on the opening of each storage slot. A temperature sensor for detecting the temperature inside the sealed storage container is provided on the inner side of the sealing cap. A mesh storage container with an outer opening is provided inside the sealed storage container. A connecting ring is provided at the opening of the mesh storage container. An annular groove that mates with the connecting ring is provided on the inner side of the sealing cap. Multiple circumferentially distributed elastic locking strips are connected to one outer edge of the annular groove, extending into it and capable of elastically deforming to allow the connecting ring to enter the annular groove. This invention enables unified and centralized cryopreservation management of samples, facilitates retrieval and operation, and effectively prevents sample damage.

Description

Technical Field

[0001] This invention belongs to the technical field of cryopreservation equipment and relates to a long-term cryopreservation equipment for biological germplasm resources. Background Technology

[0002] Against the backdrop of biodiversity conservation and the sustainable development of modern animal husbandry, the long-term, safe, and efficient preservation of biological germplasm resources, as important carriers of genetic information, has become a crucial aspect of agricultural science and life science research. Biological germplasm resources include sperm, eggs, embryos, somatic cells, and tissue samples, and are widely used in areas such as superior breed selection, genetic improvement, endangered species conservation, and emergency recovery from disease outbreaks. Taking the Balmi cattle as an example, this breed, due to its strong heat resistance, good disease resistance, and excellent meat quality, has significant economic value and promising prospects for promotion in southern my country and tropical and subtropical regions. To ensure the continuation and utilization of its high-quality genetic resources, long-term cryopreservation of Balmi cattle semen, embryos, and other germplasm materials is particularly necessary.

[0003] Currently, the long-term preservation of biological germplasm resources mainly relies on cryogenic freezing technology. Liquid nitrogen (-196℃) is widely used for the cryopreservation of various germplasm materials due to its stable low-temperature environment and good biocompatibility. The conventional approach is to place the sealed samples in insulated containers such as Dewar flasks or liquid nitrogen tanks, relying on the liquid nitrogen gas or liquid phase to maintain the low temperature. However, existing preservation equipment mostly uses traditional insulated tanks for static storage. While these have good insulation performance, they have significant limitations in practical applications: firstly, the internal structure of the tanks is simple, lacking standardized and modular sample storage units, making it difficult to achieve systematic and information-based management of large batches of samples; secondly, when specific samples need to be extracted, after the operator opens the tank lid, because the ultra-low temperature liquid nitrogen cannot be directly contacted, specific equipment is required to remove the sample from the tank, which is inefficient, inconvenient, and prone to damaging the samples. Summary of the Invention

[0004] The purpose of this invention is to provide a long-term cryopreservation device for biological germplasm resources, which can perform unified and centralized cryopreservation management of samples, is easy to handle and operate, and effectively prevents sample damage.

[0005] To address the aforementioned technical problems, this invention provides a long-term cryopreservation device for biological germplasm resources, comprising an insulated storage rack, a plurality of storage slots being provided inwardly on the front side of the insulated storage rack, a display screen being provided at each storage slot on the front side of the insulated storage rack, a sealed storage container with a front opening being detachably connected to each storage slot, a sealing cap being detachably connected to the opening end of the sealed storage container, a cap groove being provided outwardly on the opening end of each storage slot to cooperate with the sealing cap, and a temperature sensor for detecting the temperature inside the sealed storage container being provided on the inner side of the sealing cap; Each sealed storage container is equipped with a mesh storage bin with an open outer end. Each mesh storage bin has a connecting ring at its open end. The inner side of each sealing cover is equipped with an annular groove that mates with the connecting ring. Each annular groove has multiple elastic clips extending into it and distributed in a circular pattern on one outer edge. Each elastic clip is approximately V-shaped and can allow the connecting ring to enter the annular groove through elastic deformation.

[0006] By adopting the above technical solution, when it is necessary to store biological germplasm resources, first grasp the handle to remove the corresponding sealing cap from the cap slot, and then pull the mesh storage container connected to the sealing cap out of the sealed storage container. Then, place the cryopreservation tube containing the biological germplasm resources into the mesh storage container, and then press the connecting ring into the annular groove on the sealing cap. The elastic deformation of the elastic strip will lock it in place. After that, screw the sealing cap on, and then push the entire sealed storage container into the corresponding storage slot. The sealing cap will then be locked into the cap slot to complete the positioning. When it is necessary to take out a specific sample, simply pull the corresponding sealed storage container out of the storage slot, open the sealing cap, and you can directly take out the target sample from the mesh storage container. You can also manually pry the mesh storage container to make the connecting ring disengage from the annular groove and take out the target sample. There is no need to search for the sample extensively inside the large container.

[0007] The invention is further configured such that an annular conductive groove is provided on the outer side of each sealing cover, and a conductive ring electrically connected to the corresponding temperature sensor is provided in each annular conductive groove. A conductive sliding groove is provided inward on the inner wall of each cover groove, and a conductive collar electrically connected to the corresponding display screen is provided in each conductive sliding groove. A conductive sliding shaft that can extend out of the corresponding conductive sliding groove is slidably connected to each conductive collar. Each conductive sliding shaft is electrically connected to the corresponding conductive collar and can extend into the corresponding annular conductive groove and be electrically connected to the corresponding conductive collar. A support cap that cannot pass through the corresponding conductive collar is provided at one end of each conductive sliding shaft in the corresponding conductive sliding groove. A support spring is provided between each support cap and the bottom of the corresponding conductive sliding groove.

[0008] The present invention is further configured such that the end of each conductive slide shaft away from its support cap is rounded, and the side of each annular conductive groove facing the corresponding sealed storage container is an inclined side to facilitate the passage of the conductive slide shaft.

[0009] The invention is further configured such that the outer wall of the sealed storage container has a double-layer structure, and the space between the double layers is filled with vacuum powder.

[0010] The present invention is further configured such that a handle is provided on the outer side of the sealing cover.

[0011] The present invention is further configured such that the open end of the sealed storage container is provided with a connecting ring with external threads, and the sealing cover is provided with an annular connecting slot that mates with the connecting ring on the side facing the sealed storage container, and the inner wall of the annular connecting slot is provided with an internal thread that is threadedly connected to the connecting ring.

[0012] The present invention is further configured such that a rubber sealing ring that mates with the edge of the connecting ring is provided at the bottom of the annular connecting slot.

[0013] Compared with the prior art, the present invention has the following beneficial effects: Firstly, this invention uses a modular design to store different samples in independent sealed storage containers. Each sealed storage container has a separate storage slot on an insulated storage rack, which enables the systematic storage of large batches of samples in a partitioned manner. With the display screen corresponding to each sealed storage container, the internal temperature of the container can be displayed in real time, which facilitates information management by the staff. When taking out a target sample, only the corresponding sealed storage container needs to be taken out, without having to turn over all the samples in the large container, which greatly improves the efficiency of operation and reduces interference with the storage environment of other samples.

[0014] Secondly, the mesh storage container is connected to the inside of the sealing cover. The stored germplasm samples can be taken out simultaneously by removing the sealing cover. Combined with the V-shaped elastic clip design, the mesh storage container can be quickly and easily attached and fixed, and it can also be easily removed. The operation is simple and flexible. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the overall structure of the present invention; Figure 2 It is used to demonstrate the connection between the sealed storage container, the sealing cap, and the mesh storage bucket; Figure 3 This is a schematic diagram of the overall structure used to show the inner structure of the sealing cap; Figure 4 This is a schematic diagram showing the overall structure of the outer side of the sealing cap; Figure 5 It is a partial sectional view used to show the conductive collar and conductive slide shaft inside the conductive slide groove.

[0016] The components include: 1. Insulated storage rack; 2. Storage slot; 4. Display screen; 5. Sealed storage container; 6. Sealed lid; 7. Connecting ring; 8. Annular connecting slot; 9. Handle; 10. Lid groove; 11. Temperature sensor; 12. Conductive groove; 13. Conductive ring; 14. Conductive slide groove; 15. Conductive collar; 16. Conductive slide shaft; 17. Support cap; 18. Support spring; 19. Mesh storage container; 20. Connecting retaining ring; 21. Annular retaining groove; 22. Elastic retaining strip. Detailed Implementation

[0017] The following detailed description, in conjunction with the accompanying drawings and specific embodiments, provides a further detailed explanation of the long-term cryopreservation device for biological germplasm resources proposed in this invention. The advantages and features of this invention will become clearer from the following description. It should be noted that the accompanying drawings are all in a very simplified form and use non-precise proportions, and are only used to facilitate and clarify the illustration of the embodiments of this invention. The same or similar reference numerals in the drawings represent the same or similar parts.

[0018] Example, refer to Figure 1-5 A long-term cryopreservation device for biological germplasm resources includes an insulated storage rack 1 with multiple storage slots 2 for holding items opened inward on the front side of the rack 1. On the front side of the rack 1, corresponding to each storage slot 2, an independent display screen 4 is installed to display relevant information. Each storage slot 2 has a detachably connected sealed storage container 5 with an opening at the front end. The outer wall of this sealed storage container 5 adopts a special double-layer structure design, and the cavity between the two layers is filled with vacuum powder with excellent thermal insulation properties to minimize external heat transfer.

[0019] The open end of the sealed storage container 5 is detachably connected to a sealing cap 6. To achieve a tight connection, a threaded connecting ring 7 is provided on the outside of the open end of the sealed storage container 5. Correspondingly, on the side of the sealing cap 6 facing the sealed storage container 5, an annular connecting slot 8, matching the shape of the connecting ring 7, is formed inward. The inner wall of the annular connecting slot 8 is machined with internal threads, allowing it to engage with the external threads on the connecting ring 7, thereby firmly fixing the sealing cap 6 to the sealed storage container 5. To ensure absolute sealing, a rubber sealing ring (not shown) is also provided at the bottom of the annular connecting slot 8. When the connecting ring 7 is tightened, its edge fits tightly against the rubber sealing ring, forming an effective sealing barrier. For ease of operation, a handle 9 is also installed on the outside of the sealing cap 6. Furthermore, each storage slot 2 has a cover groove 10 extending outward from its open end, matching the shape of the sealing cap 6. When the sealing cap 6 is closed, part of its structure can be embedded in this groove. A temperature sensor 11 is installed on the inside of the sealing cap 6 to detect the internal temperature of the sealed storage container 5 in real time.

[0020] To achieve contactless transmission of electrical signals, an annular conductive groove 12 is formed around the outer surface of each sealing cover 6. A conductive ring 13 is embedded inside this annular conductive groove 12, and this conductive ring 13 is electrically connected to the aforementioned temperature sensor 11 via internal wiring. Correspondingly, a conductive sliding groove 14 is formed inward on the inner wall of each cover groove 10. A conductive collar 15 is installed in each conductive sliding groove 14, and this conductive collar 15 is electrically connected to the corresponding display screen 4 via wiring. A conductive sliding shaft 16 is slidably connected inside each conductive collar 15. This conductive sliding shaft 16 can slide along the inner cavity of the conductive collar 15 and can extend outward from the corresponding conductive sliding groove 14. When the sealing cover 6 is fully closed and embedded in the cover groove 10, the end of the extended conductive shaft 16 can precisely extend into the corresponding annular conductive groove 12 on the sealing cover 6 and electrically connect with the conductive ring 13 therein, thereby establishing a circuit path between the temperature sensor 11 and the display screen 4. Each conductive slide shaft 16 has a support cap 17 fixed at one end inside the conductive groove 14. The support cap 17 has a diameter larger than the inner diameter of the conductive collar 15. This support cap 17 prevents the conductive slide shaft 16 from completely falling out of the conductive collar 15. A support spring 18 is installed between the support cap 17 and the bottom of the corresponding conductive groove 14. This spring is normally compressed and provides an outward elastic force for the conductive slide shaft 16, ensuring that it can stably contact the conductive ring 13. The end of each conductive slide shaft 16 away from its support cap 17 (i.e., the outer end) is designed to be rounded to facilitate insertion and reduce friction. At the same time, the edge of each annular conductive groove 12 facing the sealed storage container 5 is machined into a bevel. This bevel design facilitates the smooth sliding of the rounded conductive slide shaft 16 into the conductive groove 12.

[0021] Inside each sealed storage container 5, there is also a mesh storage bin 19 with an open end for holding items that need to be stored. Each mesh storage bin 19 has a connecting ring 20 at its open end. On the inside of each sealed cover 6, there is an annular groove 21 that matches the shape of the connecting ring 20. To facilitate quick installation and fixation of the mesh storage bin 19, multiple elastic clips 22, extending into the groove and evenly distributed circumferentially, are connected to one outer edge of each annular groove 21. These elastic clips 22 are approximately V-shaped and possess a certain degree of elasticity. When it is necessary to fix the mesh storage bucket 19, simply align the connecting ring 20 at its open end and press it into the annular groove 21. The compression of the connecting ring 20 will cause the V-shaped elastic strips 22 to undergo outward elastic deformation, thereby allowing the connecting ring 20 to pass through. When the connecting ring 20 is fully inserted into the groove, the elastic strips 22 will return to their original shape, and the structure at their ends will lock the connecting ring 20 to prevent it from falling off, thereby achieving detachable suspension fixation of the mesh storage bucket 19 on the sealing cover 6.

[0022] Usage: When storing biological germplasm resources, first grasp the handle 9 to remove the corresponding sealing cap 6 from the cap groove 10. Then, simultaneously pull the mesh storage container 19 connected to the sealing cap 6 out of the sealed storage container 5. Next, place the cryopreservation tube containing the biological germplasm resources into the mesh storage container 19. Then, align the connecting ring 20 with the annular groove 21 on the sealing cap 6 and press it in, relying on the elastic deformation of the elastic strip 22 to secure it. Afterward, screw the sealing cap 6 to connect it to the connecting ring 7 of the sealed storage container 5 via a threaded seal. The rubber sealing ring further ensures the airtightness of the container. Then, push the entire sealed storage container 5 into the corresponding storage slot 2. The sealing cap 6 is then engaged in the cap groove 10 for positioning. At this time, the conductive slide shaft 16 extends under the action of the support spring 18, extending into the annular conductive groove 1. 2. The conductive ring 13 contacts the circuit, and the temperature inside the container detected by the temperature sensor 11 is directly transmitted to the display screen 4 next to the corresponding slot, which is convenient for the operator to check at any time and complete the storage of a single sample. When a specific sample needs to be taken out, simply pull the corresponding sealed storage container 5 out of the storage slot 2, open the sealing cover 6, and the target sample in the mesh storage bucket 19 can be taken out directly. The connecting ring 20 can be disengaged from the annular slot 21 by manually bending the mesh storage bucket 19, and the target sample can be taken out. There is no need to search for the sample in a large container. The operation is simple and convenient and will not affect the storage temperature of the samples in other storage slots 2. It realizes the modular classification management of batch samples. With the independent heat insulation structure of each sealed storage container 5, the stability of germplasm resource preservation is effectively guaranteed.

[0023] It should also be noted that all terms such as "set up" and similar descriptive words in this application (especially the specification) indicate that two structures have or exist a connection relationship. However, the specific means by which the two are connected are not limited in detail, and are usually conventional connection methods. That is, the means should be understood as prior art and do not need to be elaborated. For example, "m is set up with n" only indicates that structure m has structure n, and whether the two are connected by welding, riveting, adhesive, or integral molding is within the scope of protection of this application. Similarly, "x is rotatably set up with y" only indicates that y and x can rotate relative to each other, and whether the two are connected by a bearing, or whether y directly passes through x and is rotatably connected to x, or other feasible methods, are all within the scope of protection of this application.

[0024] The above description is merely a description of preferred embodiments of the present invention and is not intended to limit the scope of the present invention in any way. Any changes or modifications made by those skilled in the art based on the above disclosure shall fall within the protection scope of the claims.

Claims

1. A long-term cryopreservation device for biological germplasm resources, comprising an insulated storage rack (1), characterized in that, The heat-insulating storage rack (1) has multiple storage slots (2) on its front side. A display screen (4) is provided at each storage slot (2) on the front side of the heat-insulating storage rack (1). A sealed storage container (5) with a front opening is detachably connected to each storage slot (2). A sealing cap (6) is detachably connected to the opening end of the sealed storage container (5). A cover groove (10) that cooperates with the sealing cap (6) is provided outward at the opening end of each storage slot (2). A temperature sensor (11) for detecting the temperature inside the sealed storage container (5) is provided on the inside of the sealing cap (6). Each sealed storage container (5) is provided with a mesh storage bucket (19) with an open end. Each mesh storage bucket (19) has a connecting ring (20) at its open end. Each sealed cover (6) has an annular groove (21) on its inner side that cooperates with the connecting ring (20). Each annular groove (21) has a plurality of elastic strips (22) that extend into it and are distributed in a circular pattern on one outer side. Each elastic strip (22) is approximately V-shaped and can allow the connecting ring (20) to enter the annular groove (21) through elastic deformation.

2. The biological germplasm resource long-term cryopreservation equipment according to claim 1, characterized in that, Each sealing cover (6) has an annular conductive groove (12) on its outer side. Each annular conductive groove (12) has a conductive ring (13) electrically connected to the corresponding temperature sensor (11). Each cover groove (10) has a conductive sliding groove (14) on its inner wall. Each conductive sliding groove (14) has a conductive collar (15) electrically connected to the corresponding display screen (4). Each conductive collar (15) is slidably connected to a conductive sliding shaft (16) that can extend out of the corresponding conductive sliding groove (14). Each conductive sliding shaft (16) is electrically connected to the corresponding conductive collar (15) and can extend into the corresponding annular conductive groove (12) and be electrically connected to the corresponding conductive ring (13). Each conductive sliding shaft (16) has a support cap (17) that cannot pass through the corresponding conductive collar (15) at one end in the corresponding conductive sliding groove (14). Each support cap (17) is connected to the bottom of the corresponding conductive sliding groove (14) with a support spring (18).

3. The biological germplasm resource long-term cryopreservation equipment according to claim 2, characterized in that, Each conductive slide (16) has a rounded end away from its support cap (17), and each annular conductive groove (12) has an inclined side facing the corresponding sealed storage container (5) to facilitate the passage of the conductive slide (16).

4. The biological germplasm resource long-term cryopreservation equipment according to claim 1, characterized in that, The outer wall of the sealed storage container (5) has a double-layer structure, and the space between the two layers is filled with vacuum powder.

5. The biological germplasm resource long-term cryopreservation equipment according to claim 1, characterized in that, A handle (9) is provided on the outside of the sealing cover (6).

6. The biological germplasm resource long-term cryopreservation equipment according to claim 1, characterized in that, The sealed storage container (5) has an externally threaded connecting ring (7) at the open end. The sealing cover (6) has an annular connecting slot (8) that mates with the connecting ring (7) on the side facing the sealed storage container (5). The inner wall of the annular connecting slot (8) has an internal thread that is threaded to the connecting ring (7).

7. The biological germplasm resource long-term cryopreservation equipment according to claim 6, characterized in that, The bottom of the annular connecting slot (8) is provided with a rubber sealing ring that matches the edge of the connecting ring (7).

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