A liquid fertilizer containing polyglucosidase HP-JDQ2 and a preparation method and application thereof
Patent Information
- Application Number
- CN202610798622.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-06-04
- Publication Date
- 2026-08-18
AI Technical Summary
[0005]为了克服现有聚多曲霉液体肥存在的菌株适配性差、土壤改善效果差、产品储存期活菌存活率低等问题，本申请提供一种包含聚多曲霉HP-JDQ2的液体肥
1. 本申请通过使用拮抗谱广、定殖能力强、土壤适应能力好的聚多曲霉HP-JDQ2作为主要微生物成分，有机营养基质、矿物营养组分等作为辅助成分，获得的包含聚多曲霉HP-JDQ2的液体肥能够应用于番茄等果蔬及大田作物的土壤，并显著抑制土壤中尖孢镰刀菌、立枯丝核菌、烟草疫霉病原菌和胡萝卜软腐果胶杆菌等病原菌的繁殖，同时还能改良土壤理化性质、降低土壤容重，提升土壤有机质含量，有效缓解土壤板结及连作障碍，促进作物生长，实现病害防治、土壤肥力提升及促进植物生长的多重效果。
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Abstract
Description
Technical Field
[0001] This application relates to the field of microbial preparation technology, specifically to a liquid fertilizer containing Aspergillus polypolysaccharide HP-JDQ2, its preparation method, and its application. Background Technology
[0002] With the popularization of facility agriculture and continuous cropping, soil-borne diseases have become increasingly prominent. Fusarium oxysporum, Rhizoctonia solani, Phytophthora, and other pathogenic microorganisms causing wilt, damping-off, and root rot, as well as soft rot caused by Pectinobacillus carotenoides, have become important factors restricting crop yield and quality.
[0003] Currently, the control of soil diseases mainly relies on chemical pesticides such as fungicides and disinfectants. However, long-term and excessive use of chemical pesticides can lead to soil microecological imbalance, pathogen resistance, and pesticide residues that pollute soil and water bodies, seriously affecting agricultural product safety and the ecological environment. In recent years, biological control has become a research hotspot for soil disease control due to its environmental friendliness, low resistance rate, and ability to improve the soil environment. Among them, Aspergillus polypolysaccharide, as a multifunctional microorganism, has been widely used in agriculture due to its phosphorus-solubilizing, potassium-releasing, and pathogen-antagonistic properties. However, existing Aspergillus polypolysaccharide fertilizers generally suffer from poor strain compatibility, poor soil improvement effects, and low survival rate of live bacteria during product storage, making them unsuitable for the soil improvement needs of greenhouse vegetables and continuously cropped field crops.
[0004] Therefore, screening out a strain of Aspergillus polypolysaccharide with a broad antagonistic spectrum, strong colonization ability, and good soil adaptability, and preparing a liquid fertilizer, is of great significance for solving the problems of soil degradation and continuous cropping obstacles. Summary of the Invention
[0005] In order to overcome the problems of poor strain compatibility, poor soil improvement effect and low survival rate of live bacteria during product storage in existing Aspergillus polypolysaccharide liquid fertilizers, this application provides a liquid fertilizer containing Aspergillus polypolysaccharide HP-JDQ2.
[0006] The liquid fertilizer containing Aspergillus polypolysaccharide HP-JDQ2 provided in this application adopts the following technical solution: A liquid fertilizer containing Aspergillus polypolysaccharide HP-JDQ2 comprises components with the following content: viable count ≥1×10⁻⁶ 8 The formula consists of Aspergillus polypolysaccharide HP-JDQ2 (CFU / mL), organic nutrient matrix 25-45 g / L, mineral nutrient components 2-9 g / L, synergistic agent 6-17.5 g / L, preservative 0.5-1.5 g / L, with the balance being water.
[0007] This application provides a liquid fertilizer containing Aspergillus polypolysaccharide HP-JDQ2, which effectively solves the problems of low strain activity, poor soil improvement and disease suppression effects, and traditional single nutrient substrate in existing microbial liquid fertilizers. Specifically, the Aspergillus polypolysaccharide HP-JDQ2 contained in this application has advantages such as a broad antagonistic spectrum, strong colonization ability, and good soil adaptability compared to ordinary Aspergillus polypolysaccharide strains. When the liquid fertilizer containing Aspergillus polypolysaccharide HP-JDQ2 provided in this application is applied to tomatoes and other fruits and vegetables as well as field crops, it can significantly inhibit the reproduction of pathogens such as Fusarium oxysporum, Rhizoctonia solani, Phytophthora tobaccois, and Pectinobacter carotene in the soil. At the same time, it can also improve the physical and chemical properties of the soil, reduce soil bulk density, increase soil organic matter content, effectively alleviate soil compaction and continuous cropping obstacles, promote crop growth, and achieve multiple effects of disease control, soil fertility improvement, and plant growth promotion.
[0008] In some embodiments, the viable count of Aspergillus polypolysaccharide HP-JDQ2 in the liquid fertilizer can be 1~5×10⁻⁶. 8 CFU / mL, or 5×10 8 ~1×10 9 CFU / mL In one specific implementation, the viable count of Aspergillus polypolysaccharide HP-JDQ2 in the liquid fertilizer can also be 1×10⁻⁶. 8 CFU / mL, 5×10 8 CFU / mL or 1×10 9 CFU / mL.
[0009] Optionally, the Aspergillus polypolysaccharide HP-JDQ2 is deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC NO: M 2025346 and deposit date of March 3, 2025.
[0010] Optionally, the organic nutrient matrix is selected from one or more of yeast extract powder, sucrose, and soluble starch; the mineral nutrient component is selected from potassium dihydrogen phosphate and magnesium sulfate heptahydrate; the synergist is selected from one or more of xanthan gum, Tween-80, and potassium humate; and the preservative is potassium sorbate.
[0011] Optionally, the liquid fertilizer comprises components with the following content: viable bacteria count ≥ 1 × 10⁻⁶. 8 The formula consists of: Aspergillus polypolysaccharide HP-JDQ2 (CFU / mL), sucrose 15-25 g / L, soluble starch 10-20 g / L, potassium dihydrogen phosphate 1-5 g / L, magnesium sulfate heptahydrate 1-4 g / L, Tween-80 1-3 g / L, xanthan gum 0.5-1.5 g / L, potassium humate 5-13 g / L, potassium sorbate 0.5-1.5 g / L, with the balance being water.
[0012] Optionally, the liquid fertilizer may further contain seaweed hydrolysate and / or straw hydrolysate, with a total addition amount of 10-30 mL / L.
[0013] Optionally, the liquid fertilizer contains seaweed hydrolysate and straw hydrolysate, with a volume ratio of 1:(0.25-0.6).
[0014] Optionally, the volume ratio of the seaweed hydrolysate to the straw hydrolysate is 1:(0.4-0.5).
[0015] In some embodiments, the volume ratio of the seaweed hydrolysate to the straw hydrolysate can be 1:(0.25-0.4), 1:(0.25-0.5), 1:(0.25-0.6), 1:(0.4-0.5), 1:(0.4-0.6), or 1:(0.5-0.6).
[0016] In one specific implementation, the volume ratio of the seaweed hydrolysate to the straw hydrolysate can also be 1:0.25, 1:0.4, 1:0.5, or 1:0.6.
[0017] Secondly, this application provides a method for preparing a liquid fertilizer containing Aspergillus polypolysaccharide HP-JDQ2, comprising the following steps: first, preparing a liquid fertilizer with a viable count ≥1×10⁻⁶. 8 The liquid fertilizer was obtained by adding other components to the bacterial suspension of Aspergillus polypolysaccharide HP-JDQ2 at CFU / mL and stirring for 25-30 minutes until completely dissolved.
[0018] Thirdly, this application provides the application of a liquid fertilizer containing Aspergillus polypolysaccharide HP-JDQ2 in soil improvement and plant growth promotion, wherein the soil improvement includes activating soil nutrients and inhibiting soil pathogens.
[0019] In summary, this application has the following beneficial effects: 1. This application uses Aspergillus polypolysaccharide HP-JDQ2, which has a broad antagonistic spectrum, strong colonization ability, and good soil adaptability, as the main microbial component, and organic nutrient substrate and mineral nutrient components as auxiliary components. The resulting liquid fertilizer containing Aspergillus polypolysaccharide HP-JDQ2 can be applied to the soil of fruits and vegetables such as tomatoes and field crops. It significantly inhibits the reproduction of pathogens such as Fusarium oxysporum, Rhizoctonia solani, Phytophthora indicum, and Pectinobacillus carotenoides in the soil. At the same time, it can improve the physical and chemical properties of the soil, reduce the soil bulk density, increase the soil organic matter content, effectively alleviate soil compaction and continuous cropping obstacles, promote crop growth, and achieve multiple effects of disease control, soil fertility improvement, and plant growth promotion.
[0020] 2. This application further adds novel resource-based nutrient substrates such as seaweed enzymatic hydrolysate and / or straw enzymatic hydrolysate to liquid fertilizer. On the one hand, it can realize the resource utilization of agricultural waste such as straw and mushroom residue; on the other hand, it can provide balanced carbon and nitrogen nutrition for the growth of bacterial strains, prolong the survival rate of live bacteria in liquid fertilizer during storage, and synergistically improve the physical and chemical properties of soil, reduce soil bulk density, and increase soil organic matter content, ultimately achieving the effects of soil improvement and promoting crop growth. Detailed Implementation
[0021] This application provides a liquid fertilizer containing Aspergillus polypolysaccharide HP-JDQ2, comprising the following components: viable count ≥ 1 × 10⁻⁶. 8 The composition consists of *Aspergillus polypolysaccharide* HP-JDQ2 (CFU / mL), 25-45 g / L organic nutrient matrix, 2-9 g / L mineral nutrients, 6-17.5 g / L synergist, 0.5-1.5 g / L preservative, with the balance being water. *Aspergillus polypolysaccharide* HP-JDQ2 is deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC NO: M 2025346, on March 3, 2025. The organic nutrient matrix is selected from one or more of yeast extract, sucrose, and soluble starch; the mineral nutrients are selected from potassium dihydrogen phosphate and magnesium sulfate heptahydrate; the synergist is selected from one or more of xanthan gum, Tween-80, and potassium humate; and the preservative is potassium sorbate. Furthermore, the liquid fertilizer also contains seaweed hydrolysate and / or straw hydrolysate, with a total addition amount of 10-30 mL / L; even further, the volume ratio of the seaweed hydrolysate to the straw hydrolysate is 1:(0.4-0.5).
[0022] This application also provides a method for preparing the above-mentioned liquid fertilizer containing Aspergillus polypolysaccharide HP-JDQ2, including the following steps: first, preparing a liquid fertilizer with a viable count ≥1×10⁻⁶. 8 The liquid fertilizer was obtained by adding other components to the bacterial suspension of Aspergillus polypolysaccharide HP-JDQ2 at CFU / mL and stirring for 25-30 minutes until completely dissolved.
[0023] The raw materials, reagents, solvents, etc. used in this application can all be obtained commercially.
[0024] The following describes this application in further detail with reference to preparation examples, embodiments, and performance testing. Preparation Example 1
[0025] Preparation Example 1 provides an algae enzymatic hydrolysate.
[0026] The preparation method of the above-mentioned seaweed enzymatic hydrolysate is as follows: (1) Soak the dried seaweed in clean water for 2 hours and then rinse it repeatedly so that the salt content of the seaweed is <2%; then cut the cleaned seaweed into sections, add water at a solid-liquid ratio of 1:4 to make a seaweed homogenate.
[0027] (2) Transfer the seaweed homogenate to the enzymatic hydrolysis reactor, adjust the pH to 6.0 with citric acid / sodium hydroxide solution; stir and heat to 45℃; then add compound seaweed enzyme (enzyme activity ≥10000 U / g, containing alginate lyase, cellulase and protease with a live bacteria ratio of 1:2:1) at 0.5% of the total mass of seaweed homogenate, and hydrolyze at 100r / min and 45℃ for 8h. (3) After the enzymatic hydrolysis is completed, the reaction solution is rapidly heated to 90°C and kept at a constant temperature for 20 min to terminate the enzymatic hydrolysis reaction. Then, it is rapidly cooled to below 30°C and centrifuged at 5000 r / min for 15 min. The supernatant is taken and 0.2% diatomaceous earth is added as a filter aid. The solution is then filtered through a plate and frame filter and a 0.22 μm microporous membrane to obtain a clear and transparent seaweed enzymatic hydrolysate. Preparation Example 2
[0028] Preparation Example 2 provides a straw enzymatic hydrolysate.
[0029] The preparation method of the above-mentioned straw enzymatic hydrolysate is as follows: (1) Cut the straw into sections, crush it, and pass it through a 20-mesh sieve to obtain straw powder; then dry it with hot air so that the moisture content is <10%.
[0030] (2) Add 1% dilute sulfuric acid to the dry straw powder according to the material-liquid mass ratio of 1:8, mix evenly, put it into a high-pressure reactor, and treat it at 121℃ and 0.1MPa for 30min; after the treatment is completed, cool it to room temperature, adjust the pH to 5.0 with 2% sodium hydroxide solution to obtain pretreated straw slurry.
[0031] (3) Add water to the pretreated straw slurry to adjust the solid content to 10-12% and adjust the system temperature to 50℃; then add compound cellulase (enzyme activity ≥15000 U / g, containing cellulase, hemicellulase and ligninase with a live bacteria ratio of 1:0.5:0.3) at 0.5% of the total mass of the pretreated straw slurry, start stirring, and enzymatically hydrolyze at 120r / min and 50℃ for 14h; after the enzymatic hydrolysis is completed, raise the temperature of the liquid to 90℃ and keep it warm for 20min; then cool it to below 30℃, centrifuge at 5000r / min for 20min, take the supernatant, add 0.15% perlite as a filter aid, filter through plate and frame filter and fine filter through 0.22μm microporous membrane to obtain clear straw enzymatic hydrolysate. Examples 1-3
[0032] Examples 1-3 provide a liquid fertilizer containing Aspergillus polypolysaccharide HP-JDQ2.
[0033] The difference in the above embodiments is the number of viable Aspergillus polypolysaccharide HP-JDQ2 bacteria in the liquid fertilizer.
[0034] Examples 1-3 provide a method for preparing a liquid fertilizer containing Aspergillus polypolysaccharide HP-JDQ2, comprising the following steps: (1) Aspergillus polypolysaccharide HP-JDQ2 was activated and transferred to a seed fermentation medium. It was cultured at 30℃ for 24h to obtain the fermentation seed liquid of Aspergillus polypolysaccharide HP-JDQ2. The fermentation seed liquid was inoculated into a fermenter containing seed fermentation medium at a volume ratio of 3%. It was cultured at 30℃ and 180r / min with ventilation and shaking. After about 72h, the culture medium was observed to become viscous, the mycelium proliferated in large quantities, the color was dark brown, and there was no odor. Fermentation was completed. The fermentation liquid of Aspergillus polypolysaccharide HP-JDQ2 was centrifuged at a speed of 5000r / min. After centrifugation, the supernatant and precipitate were obtained. The precipitate was Aspergillus polypolysaccharide HP-JDQ2 cells. The bacterial content was detected by dilution plating method. Finally, the cells were diluted with sterile water to obtain a suspension of Aspergillus polypolysaccharide HP-JDQ2 cells.
[0035] (2) Add 20g of sucrose, 15g of soluble starch, 3g of potassium dihydrogen phosphate, 2g of magnesium sulfate heptahydrate, 2g of Tween-80, 1g of xanthan gum, 10g of potassium humate and 1g of potassium sorbate to 1L of Aspergillus polypolysaccharide HP-JDQ2 bacterial suspension, stir for 30min until completely dissolved to obtain liquid fertilizer.
[0036] The viable counts of Aspergillus polypolysaccharide HP-JDQ2 bacterial suspensions obtained in steps (1) of Examples 1-3 were 1×10⁻⁶. 8 CFU / mL, 5×10 8 CFU / mL, 1×10 9 Therefore, the viable count of Aspergillus polypolysaccharide HP-JDQ2 in the liquid fertilizers obtained in Examples 1-3 was 1×10⁻⁶ CFU / mL. 8 CFU / mL, 5×10 8 CFU / mL, 1×10 9 CFU / mL. Example 4
[0037] Example 4 provides a liquid fertilizer containing Aspergillus polypolysaccharide HP-JDQ2.
[0038] The difference between the above embodiment and embodiment 2 is that 20 mL of seaweed enzymatic hydrolysate is added to the liquid fertilizer in step (2). Example 5
[0039] Example 5 provides a liquid fertilizer containing Aspergillus polypolysaccharide HP-JDQ2.
[0040] The difference between the above embodiment and embodiment 2 is that 20 mL of straw enzymatic hydrolysate is added to the liquid fertilizer in step (2). Example 6
[0041] Example 6 provides a liquid fertilizer containing Aspergillus polypolysaccharide HP-JDQ2.
[0042] The difference between the above embodiment and embodiment 2 is that 20 mL of a mixture of seaweed hydrolysate and straw hydrolysate with a volume ratio of 1:0.25 is added to the liquid fertilizer in step (2). Example 7
[0043] Example 7 provides a liquid fertilizer containing Aspergillus polypolysaccharide HP-JDQ2.
[0044] The difference between the above embodiment and embodiment 2 is that 20 mL of a mixture of seaweed hydrolysate and straw hydrolysate with a volume ratio of 1:0.4 is added to the liquid fertilizer in step (2). Example 8
[0045] Example 8 provides a liquid fertilizer containing Aspergillus polypolysaccharide HP-JDQ2.
[0046] The difference between the above embodiment and embodiment 2 is that 20 mL of a mixture of seaweed hydrolysate and straw hydrolysate with a volume ratio of 1:0.5 is added to the liquid fertilizer in step (2). Example 9
[0047] Example 9 provides a liquid fertilizer containing Aspergillus polypolysaccharide HP-JDQ2.
[0048] The difference between the above embodiment and embodiment 2 is that 20 mL of a mixture of seaweed hydrolysate and straw hydrolysate with a volume ratio of 1:0.6 is added to the liquid fertilizer in step (2). Comparative Example 1
[0049] Comparative Example 1 provides a liquid fertilizer containing Aspergillus polypolysaccharide HP-JDQ2.
[0050] The difference between the above comparative example and Example 2 is the number of viable Aspergillus polypolysaccharide HP-JDQ2 bacteria in the liquid fertilizer.
[0051] The viable count of Aspergillus polypolysaccharide HP-JDQ2 suspension obtained in step (1) of Comparative Example 1 was 1 × 10⁻⁶. 7 Therefore, the viable count of Aspergillus polypolysaccharide HP-JDQ2 in the liquid fertilizer obtained in Comparative Example 1 was 1 × 10⁻⁶ CFU / mL. 7 CFU / mL. Comparative Example 2
[0052] Comparative Example 2 provides a liquid fertilizer containing Aspergillus polypolysaccharide JDQMZZ-1.
[0053] The difference between the above comparative example and Example 2 is that the Aspergillus polypolypeptide HP-JDQ2 bacterial suspension was replaced with Aspergillus polypolypeptide JDQMZZ-1 bacterial suspension; the viable count of the Aspergillus polypolypeptide JDQMZZ-1 bacterial suspension was 5 × 10⁻⁶. 8 CFU / mL. Performance testing experiment
[0054] The effects of Aspergillus polypolysaccharide liquid fertilizers provided in Examples 1-9 and Comparative Examples 1-2 on disease resistance and growth promotion of tomato plants were investigated. The specific experimental procedures are as follows: (1) Experimental soil: Soil from greenhouses where tomatoes have been continuously grown for more than 5 years was selected as the experimental area. The basic physicochemical properties of the soil in this area were: pH 5.8~6.0, organic matter 12.5 g / kg, available phosphorus 18.2 mg / kg, available potassium 85.3 mg / kg, and soil bulk density 1.42 g / cm³. 3 The content of Fusarium oxysporum was 3.7 × 10⁻⁶. 4 CFU / g.
[0055] (2) The soil in each plot was deeply tilled to a depth of 25-30 cm, leveled, and then ridged with ridges 20 cm high and 60 cm apart. 40 tomato seedlings were planted in each plot with a plant spacing of 30 cm. After planting, the seedlings were thoroughly watered. The experimental area was then divided into 12 plots, including 9 experimental plots (experimental plots 1-9), 2 control plots (control plots 1-2), and 1 blank control plot.
[0056] (3) Ten and twenty days after transplanting, Aspergillus polymorpha liquid fertilizer was applied to each area by irrigation. The fertilization method was to dilute the Aspergillus polymorpha liquid fertilizer 500 times and irrigate the roots, with 500 mL applied to each plant. Among them, nine experimental areas were irrigated with Aspergillus polymorpha HP-JDQ2 liquid fertilizer provided in Examples 1-9; two control areas were irrigated with Aspergillus polymorpha liquid fertilizer provided in Comparative Examples 1-2; and no fertilizer was applied to the blank control area, only water was applied. Daily management: Throughout the entire experimental period, the management measures such as temperature and humidity, pest and disease control, and watering were kept consistent in each plot.
[0057] (4) Soil microbial testing: Soil samples were taken before planting, 15 days after the first fertilization, and after tomato harvest. The soil in the 0-20cm topsoil layer of each plot was collected using the "five-point sampling method". After removing impurities, the soil was mixed evenly and the number of Fusarium oxysporum in the soil was determined. The results are shown in Table 1 below.
[0058] (5) Crop growth indicators: During the tomato harvest period, the plant height (from the base to the top of the main stem) and stem diameter (5 cm from the base) of the tomato seedlings were measured. After the tomato harvest, the tomatoes in each area were weighed, and the average yield and average weight of a single tomato plant in each area were calculated. The results are shown in Table 1 below.
[0059] Table 1 Soil parameters and crop yields in each experimental and control area
[0060] According to the test results in Table 1, after applying the Aspergillus polypolysaccharide HP-JDQ2 liquid fertilizer provided in Example 1 and planting tomatoes, the Fusarium oxysporum content in the soil of experimental areas 1-9 was reduced to 3.2 × 10⁻⁶. 2 ~9.1×10 2 The CFU / g concentration of the tomato plants was 216.1-236.5 cm in height and 1.2-1.4 cm in stem diameter; the yield per plant was 5.11-5.64 kg, and the weight of a single fruit was 216.3-232.8 g; while the control area 1 used the viable bacteria count provided by Comparative Example 1, which was 1×10⁻⁶. 7 After applying CFU / mL Aspergillus polypolysaccharide HP-JDQ2 liquid fertilizer and planting tomatoes, the soil Fusarium oxysporum content was reduced to 4.6 × 10⁻⁶. 3 The CFU / g count of the tomato plants was 174.7 cm in height and 1.0 cm in stem diameter; the yield per plant was 4.43 kg, and the weight of a single fruit was 208.7 g; while the control area 2 used the viable bacteria count provided by Comparative Example 2 was 5 × 10⁻⁶. 8 After applying liquid fertilizer containing CFU / mL Aspergillus polypolysaccharide JDQMZZ-1 and planting tomatoes, the soil Fusarium oxysporum content was reduced to 7.2 × 10⁻⁶. 3 The tomato plant height was 158.4 cm and the stem diameter was 1.0 cm; the yield per plant was 4.04 kg, and the weight of a single fruit was 197.1 g. Therefore, this application demonstrates that the liquid fertilizer was prepared using *Aspergillus polypolysaccharide* HP-JDQ2, and the viable bacterial count in the liquid fertilizer was controlled to be ≥1×10⁻⁶. 8 The CFU / mL polyaspergillus liquid fertilizer can effectively improve the soil, inhibit the reproduction of pathogens such as Fusarium oxysporum in the soil, alleviate soil compaction and continuous cropping obstacles, promote crop growth, and achieve multiple effects of disease control, soil fertility improvement and plant growth promotion.
[0061] Although the present invention has been described in detail above with general descriptions and specific embodiments, modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention fall within the scope of protection claimed by the present invention.
Claims
1. A liquid fertilizer comprising Polyglutamic Acid HP-JDQ2, characterized in that, comprising the following components: viable cell count ≥ 1 x 10 8 Polyglucosidase HP-JDQ2 1 x 10 CFU / mL, organic nutrient substrate 25-45 g / L, mineral nutrient components 2-9 g / L, synergistic adjuvants 6-17.5 g / L, preservatives 0.5-1.5 g / L, the balance being water.
2. The liquid manure comprising Polyangium HP-JDQ2 according to claim 1, characterized by, The Aspergillus polypolysaccharide HP-JDQ2 strain is deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC NO: M 2025346 and deposit date of March 3, 2025.
3. The liquid manure comprising Polyangium HP-JDQ2 according to claim 2, characterized by, The organic nutrient matrix is selected from one or more of yeast extract powder, sucrose, and soluble starch; the mineral nutrient component is selected from potassium dihydrogen phosphate and magnesium sulfate heptahydrate; the synergist is selected from one or more of xanthan gum, Tween-80, and potassium humate; and the preservative is potassium sorbate.
4. The liquid fertilizer containing Aspergillus polypolysaccharide HP-JDQ2 according to any one of claims 1-3, characterized in that, The liquid fertilizer comprises the following components: viable bacterial count ≥1×10 8 CFU / mL of Polypleurotus HP-JDQ2, sucrose 15-25 g / L, soluble starch 10-20 g / L, potassium dihydrogen phosphate 1-5 g / L, magnesium sulfate heptahydrate 1-4 g / L, Tween-80 1-3 g / L, xanthan gum 0.5-1.5 g / L, potassium humate 5-13 g / L, potassium sorbate 0.5-1.5 g / L, and the balance being water.
5. The liquid fertilizer containing Aspergillus polypolysaccharide HP-JDQ2 according to claim 4, characterized in that, The liquid fertilizer also contains seaweed hydrolysate and / or straw hydrolysate, with a total addition amount of 10-30 mL / L.
6. The liquid fertilizer containing Aspergillus polypolysaccharide HP-JDQ2 according to claim 5, characterized in that, The liquid fertilizer contains seaweed hydrolysate and straw hydrolysate, with a volume ratio of 1:(0.25-0.6).
7. The liquid fertilizer containing Aspergillus polypolysaccharide HP-JDQ2 according to claim 6, characterized in that, The volume ratio of the seaweed hydrolysate to the straw hydrolysate is 1:(0.4-0.5).
8. The method for preparing liquid fertilizer containing Aspergillus polypolysaccharide HP-JDQ2 as described in any one of claims 1-7, characterized in that, Includes the following steps: First, prepare a polyglutamic acid-producing Aspergillus HP-JDQ2 bacterial suspension with a viable bacterial count of ≥1×10 8 CFU / mL, then add other components to the bacterial suspension and stir for 25-30 min until completely dissolved to obtain a liquid fertilizer.
9. The application of the liquid fertilizer containing Aspergillus polypolysaccharide HP-JDQ2 as described in any one of claims 1-7 in soil improvement and plant growth promotion, characterized in that, The soil improvement includes activating soil nutrients and inhibiting soil pathogens.