A tebuconazole-containing fertilizer product and a method for preparing the same

Abstract

The application belongs to the technical field of medicine and fertilizer, and discloses a kind of azole-containing medicine and fertilizer product and preparation method thereof.The azole-containing medicine and fertilizer product includes 26-30 mL azole water emulsion, 0.2-0.23 g copper sulfate pentahydrate, 10-12 mL compound fermentation liquor and 40 L water;The concentration of azole in the azole water emulsion is 0.25 g / mL;The compound fermentation liquor is prepared by mixing corn flour, molasses, soybean meal, humic acid and water and then fermenting by compound bacteria, and the compound bacteria include aspergillus niger and EM bacteria.The application complexly compounds azole and copper sulfate and sprays them at the same time, reduces the burden of planting, improves the effect of rust control, and improves the yield of wheat.

Description

Technical Field

[0001] This invention belongs to the field of pesticide and fertilizer technology, and in particular relates to a pesticide and fertilizer product containing tebuconazole and its preparation method. Background Technology

[0002] Rust infection damages leaf photosynthetic tissue, hindering nutrient synthesis. Tebuconazole can inhibit the biosynthesis of ergosterol, a key component of the rust fungus cell membrane, thereby disrupting the fungal cell structure and causing the pathogen to die. Furthermore, tebuconazole has strong systemic properties, allowing it to be absorbed through leaves or stems and translocated to all parts of the plant, inhibiting pathogen spread from within. It is effective even if the pesticide solution does not completely cover the affected area. In summary, tebuconazole can directly control the spread of rust, maintain leaf function, promote grain filling, and increase yield. Simultaneously, tebuconazole also has a mild growth regulator effect, enhancing wheat's resistance to lodging, optimizing tillering structure, and indirectly promoting yield increase.

[0003] Trace amounts of copper (0.5-1.5 ppm) can activate enzyme systems in wheat, such as polyphenol oxidase and ascorbic acid oxidase, promoting the transport of photosynthetic products to the grains, increasing thousand-grain weight and grain plumpness, and thus increasing yield. Pure copper sulfate (CuSO4·5H2O) contains approximately 25.5% copper, and the effective copper concentration needs to be reduced to 0.5-1.5 ppm through dilution, i.e., preparing a copper sulfate solution of approximately 2-6 ppm, to avoid the risk of brown spots on leaves and reduced yield due to exceeding the safe copper threshold (>1.5 ppm).

[0004] When combining the two (tebuconazole and copper sulfate) to achieve better wheat yield, tebuconazole and copper sulfate solutions can be sprayed at different times. For example, tebuconazole can be sprayed first, and after an interval of more than 48 hours, when the solution has been fully absorbed, copper sulfate solution can be sprayed. However, this undoubtedly increases the planting burden. Summary of the Invention

[0005] To address the aforementioned problems, this invention proposes a tebuconazole-containing fertilizer product and its preparation method. Tebuconazole is compounded with copper sulfate and sprayed simultaneously, which reduces the planting burden while improving the control effect of rust disease and increasing wheat yield.

[0006] To achieve the above objectives, in a first aspect, the present invention provides a tebuconazole-containing fertilizer product, comprising 26-30 mL of tebuconazole water emulsion, 0.2-0.23 g of copper sulfate pentahydrate, 10-12 mL of compound fermentation broth, and 40 L of water; The concentration of tebuconazole in the tebuconazole water emulsion is 0.25 g / mL; The compound fermentation broth is prepared by fermentation of a mixture of corn flour, molasses, soybean meal, humic acid and water with compound bacteria, including Aspergillus niger and EM bacteria.

[0007] Furthermore, the preparation method of the composite fermentation broth is as follows: A1. By weight, mix 30-35 parts corn flour, 8-10 parts molasses, 16-20 parts soybean meal, 5-6 parts humic acid and 30-35 parts water to obtain the base material; A2. Mix Aspergillus niger and EM bacteria at a mass ratio of 1:(0.8-1) to obtain a compound bacteria; A3. Adjust the pH of the substrate obtained in A1 to 5.0-5.5 with citric acid, sterilize and cool, inoculate with the compound bacteria obtained in A2, the mass of which is 1.5%-2% of the substrate mass, ferment, filter, and obtain the compound fermentation broth.

[0008] Furthermore, in A3, the sterilization temperature is 120±2℃, and the sterilization time is 15-20min.

[0009] Furthermore, in A3, the specific fermentation process is as follows: ferment at 28-30℃ with ventilation for 5-7 days, stirring 2-3 times daily.

[0010] Furthermore, the EM bacteria contain lactic acid bacteria and yeast.

[0011] Furthermore, the humic acid is fulvic acid.

[0012] Furthermore, the tebuconazole-containing fertilizer product is used for wheat planting, and is sprayed twice consecutively, once at the wheat jointing stage and once at the heading stage, with a spraying amount of 40±2L / mu each time.

[0013] Secondly, the present invention provides a method for preparing the above-mentioned tebuconazole-containing fertilizer product, wherein copper sulfate pentahydrate is first added to water and stirred, then a compound fermentation liquid is added and stirred continuously, and finally tebuconazole water emulsion is added and stirred continuously until mixed, thereby obtaining the tebuconazole-containing fertilizer product.

[0014] Furthermore, the stirring speed is 100-120 r / min.

[0015] Compared with the prior art, the present invention has the following beneficial effects: The tebuconazole-containing fertilizer product of this invention is obtained by compounding tebuconazole water emulsion, copper sulfate pentahydrate, compound fermentation broth and water. Tebuconazole can directly control the spread of rust disease, maintain leaf function, promote grain filling and increase yield. At the same time, it also has a mild growth regulator effect, which can enhance the lodging resistance of wheat, optimize the tillering structure and indirectly promote yield increase.

[0016] In the preparation of the compound fermentation broth, the yeast contained in EM bacteria can synergistically work with Aspergillus niger, providing an additional carbon source for Aspergillus niger through metabolic byproducts (such as ethanol), indirectly promoting citric acid accumulation. The lactic acid bacteria contained in EM bacteria can maintain the acidic environment, inhibiting other bacteria, and competitively consume sugar sources, indirectly limiting excessive substrate consumption by Aspergillus niger, thereby optimizing citric acid production efficiency. Citric acid itself can participate in the tricarboxylic acid cycle, improve leaf ATP synthesis efficiency, accelerate the distribution of photosynthetic products to grains, and promote yield increase.

[0017] The tricarboxylic acid group of citric acid can preferentially react with Cu. 2+ It forms a soluble complex, occupies the copper binding site, protects the molecular structure of tebuconazole, and weakens / counters the negative effects of the combination and joint spraying of copper sulfate and tebuconazole. Trace amounts of copper, as a cofactor, can activate the enzyme system of wheat, such as polyphenol oxidase, promote the transport of photosynthetic products to grains, promote the accumulation of grain substances, and promote yield increase. At the same time, the humic acid (fulvic acid) contained in the compound fermentation liquid can reduce the hydrophobicity of the leaf wax layer, increase the permeability of the leaf wax layer, accelerate the tebuconazole conduction efficiency weakened by the combination, and make it more efficient to be absorbed and transported to all parts of the plant through the leaves or stems, thereby synergistically improving the bactericidal effect and synergistically increasing wheat yield. Attached Figure Description

[0018] Figure 1 This is a comparative trend chart of wheat rust control rate data in Examples 1-3 and Comparative Examples 1-3 of the experimental cases; Figure 2 This is a comparative trend chart of wheat yield growth rate data for Examples 1-3 and Comparative Examples 1-3 in the experimental cases. Detailed Implementation

[0019] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention. Example 1

[0020] (1) The preparation method of the compound fermentation broth is as follows: A1. First, prepare the raw materials: Corn flour needs to be sieved through a 60-mesh sieve (particle size ≤ 0.25mm), with a starch content ≥ 65%, to provide a carbon source. Molasses should specifically be cane molasses (total sugar ≥ 48%). Soybean meal should be ground to a 40-mesh sieve (particle size ≤ 0.42mm), with a crude protein content ≥ 44%, and pre-soaked for 2 hours to promote water absorption. Humic acid should be fulvic acid.

[0021] Then, by weight, mix 32 parts corn flour, 9 parts molasses, 18 parts soybean meal, 5.5 parts humic acid and 32 parts water. Specifically, use a twin-shaft paddle mixer (45 rpm) to dry mix the corn flour and soybean meal for 3 minutes, then add water and continue stirring while slowly adding the molasses and humic acid. The total mixing time is 15 minutes to obtain the base material.

[0022] A2. Mix Aspergillus niger and EM bacteria at a mass ratio of 1:0.9 to obtain a compound bacteria.

[0023] A3. Adjust the pH of the base material obtained in A1 to approximately 5.2 using citric acid. Specifically, prepare a 10% solution of food-grade citric acid and add it in three portions until the pH reaches approximately 5.2. After sterilizing at 120℃ for 18 minutes (0.1MPa pressure), cool to below 30℃ to avoid scalding the inoculum.

[0024] Then, the A2-derived compound bacteria were inoculated, with the mass of the compound bacteria being 1.8% of the substrate mass (the viable count was approximately 5 × 10⁻⁶). 9 Fermentation was carried out at 28-30℃ with ventilation for 6 days (0.2 vvm, dissolved oxygen ≥30%). The mixture was stirred twice daily for 5 minutes each time at 60 rpm, alternating between counterclockwise and clockwise. After 6 days of fermentation, the filtrate was obtained by filtration, which is the compound fermentation broth.

[0025] Fulvic acid (90% effective ingredient content) was purchased from Wuhan Huaxiang Kejie Biotechnology Co., Ltd. EM bacteria (type AAA) were purchased from Shandong Runhe Chemical Co., Ltd., containing lactic acid bacteria and yeast. Aspergillus niger (CICC 2238) was purchased from Shanghai Beinuo Biotechnology Co., Ltd.

[0026] (2) A method for preparing a tebuconazole-containing fertilizer product: First, add 0.22g of copper sulfate pentahydrate to 40L of water and stir at a stirring speed of 110r / min for 5min; then add 11mL of compound fermentation broth and continue stirring at a stirring speed of 110r / min for 5min; finally, add 28mL of tebuconazole water emulsion and continue stirring at a stirring speed of 110r / min for 10min to obtain the tebuconazole-containing fertilizer product. The concentration of tebuconazole in the tebuconazole water emulsion is 0.25g / mL.

[0027] This tebuconazole-containing fertilizer product is used for wheat planting. It should be sprayed twice consecutively, once during the wheat jointing stage and once during the heading stage, with a spraying amount of 40L / mu each time. Example 2

[0028] The difference between this embodiment and Embodiment 1 is as follows: A method for preparing a tebuconazole-containing fertilizer product involves first adding 0.2g of copper sulfate pentahydrate to 40L of water and stirring at a constant speed of 100r / min for 5 minutes; then adding 10mL of compound fermentation broth and continuing to stir at a constant speed of 100r / min for 5 minutes; finally adding 26mL of tebuconazole water emulsion and continuing to stir at a constant speed of 100r / min for 10 minutes to obtain the tebuconazole-containing fertilizer product. The concentration of tebuconazole in the tebuconazole water emulsion is 0.25g / mL. Example 3

[0029] The difference between this embodiment and Embodiment 1 is as follows: A method for preparing a tebuconazole-containing fertilizer product involves first adding 0.23g of copper sulfate pentahydrate to 40L of water and stirring at a constant speed of 120r / min for 5 minutes; then adding 12mL of compound fermentation broth and continuing to stir at a constant speed of 120r / min for 5 minutes; finally adding 30mL of tebuconazole water emulsion and continuing to stir at a constant speed of 120r / min for 10 minutes to obtain the tebuconazole-containing fertilizer product. The concentration of tebuconazole in the tebuconazole water emulsion is 0.25g / mL.

[0030] Comparative Example 1 The difference between this comparative example and Example 1 is that 28 mL of tebuconazole water emulsion was added to 40 L of water and stirred at a constant speed of 110 r / min for 10 min to obtain tebuconazole spray.

[0031] This tebuconazole spray is used for wheat cultivation. It is applied twice consecutively, once at the wheat jointing stage and once at the heading stage, with a spraying rate of 40L / mu each time.

[0032] Comparative Example 2 The difference between this comparative example and Example 1 is that the compound fermentation broth is removed in the preparation of the tebuconazole-containing fertilizer product.

[0033] Specifically, a method for preparing a tebuconazole-containing fertilizer product involves first adding 0.22 g of copper sulfate pentahydrate to 40 L of water and stirring at a constant speed of 110 r / min for 5 min; then adding 28 mL of tebuconazole water emulsion and stirring at a constant speed of 110 r / min for 10 min to obtain the tebuconazole-containing fertilizer product. The concentration of tebuconazole in the tebuconazole water emulsion is 0.25 g / mL.

[0034] This tebuconazole-containing fertilizer product is used for wheat planting. It should be sprayed twice consecutively, once during the wheat jointing stage and once during the heading stage, with a spraying amount of 40L / mu each time.

[0035] Comparative Example 3 The difference between this comparative example and Example 1 is that copper sulfate pentahydrate is removed in the preparation of the tebuconazole-containing fertilizer product.

[0036] Add 28 mL of tebuconazole water-in-oil emulsion to 40 L of water and stir at a constant speed of 110 rpm for 5 min. Then add 11 mL of compound fermentation broth and stir at a constant speed of 110 rpm for 10 min to obtain the tebuconazole-containing fertilizer product. The concentration of tebuconazole in the tebuconazole water-in-oil emulsion is 0.25 g / mL.

[0037] This fertilizer and pesticide containing tebuconazole is used for wheat planting. It should be sprayed twice consecutively, once at the wheat jointing stage and once at the heading stage, with a spraying amount of 40L / mu each time.

[0038] Test case Experimental groups: Blank group: sprayed with water, without adding any chemicals.

[0039] The experimental groups (Examples 1-3 and Comparative Examples 1-3) were sprayed with the corresponding products.

[0040] The field layout adopted a randomized block design with 3 replicates per group and a plot area of ​​20m². 2 Protective rows are spaced 1 meter apart; the experimental field is selected from areas with high incidence of rust, uniform soil fertility, and consistent previous crops.

[0041] Experimental Method (Operation Procedure): Two applications were made: one at the jointing stage (March 15, 2024, when basal internodes elongated by 2-3 cm), effectively preventing initial rust infection and strengthening stems; and the other at the heading stage (April 20, 2024, when 50% of ears emerged), effectively blocking pathogen spore spread and protecting flag leaf photosynthetic function. The application rate was 40 L / mu (approximately 667 square meters), with droplet size of 100-150 μm (pressure 0.3 MPa), achieving a coverage rate of ≥90%, and ensuring no rainfall within 24 hours after application.

[0042] Rust control rate: The diseased leaf rate was investigated 14 days after application of the pesticide, and the rust control rate was calculated. The calculation formula is: Rust control rate = (Diseased leaf rate in the control group - Diseased leaf rate in the experimental group) / Diseased leaf rate in the control group × 100%. Reference standard for index measurement method: Diseased leaf rate: 50 leaves were randomly selected and the percentage of diseased area was counted. The diseased leaf area ≥ 5% was counted as a diseased leaf.

[0043] Yield growth rate: 1m per plot at maturity 2 Yield (kg), record yield per mu (kg), and calculate yield growth rate; the calculation formula is: yield growth rate = (experimental group yield per mu - control group yield per mu) / control group yield per mu × 100%.

[0044] Experimental results: see Table 1.

[0045] Table 1. Statistical Analysis of Experimental Data for Experimental Examples Analysis of experimental results: Combining the data in Table 1 and Figures 1-2 Analysis of Examples 1-3 shows that the tebuconazole fertilizer product prepared by the present invention (Examples 1-3) was applied to wheat planting and sprayed twice consecutively, specifically once at the wheat jointing stage and once at the heading stage, with a spraying amount of 40L / mu each time. As a result, the rust control rate after two applications was as high as 90.2% or more, and the wheat yield growth rate was as high as 15.7% or more.

[0046] Combining the data in Table 1 and Figures 1-2 Analysis was conducted on Example 1 and Comparative Examples 1-3, specifically comparing Comparative Examples 1 and 2. It was found that, compared to Comparative Example 1 where tebuconazole was sprayed alone, Comparative Example 2, which involved adding copper sulfate pentahydrate to tebuconazole emulsion and dissolving both in water to create a tebuconazole-containing fertilizer product, showed that the rust control rate 14 days after the first application decreased from 81.3% (Comparative Example 1) to 76.8% (Comparative Example 2), and the rust control rate 14 days after the second application decreased from 89.8% (Comparative Example 1) to 74.9% (Comparative Example 2). The wheat yield growth rate also decreased from 11.8% (Comparative Example 1) to 11.2% (Comparative Example 2). This indicates that directly mixing copper sulfate pentahydrate and tebuconazole emulsion leads to a decrease in the rust control effect of tebuconazole, which is detrimental to wheat yield increase.

[0047] This is because, although spraying a mixture of tebuconazole and copper sulfate solution reduces the burden on the plant, the tebuconazole molecule contains a triazole group, which easily combines with copper ions to form a complex. This hinders the tebuconazole from penetrating into the plant tissue through stomata or epidermis, weakens its systemic conduction ability, and leads to a decrease in bactericidal efficiency. This affects the control of rust disease and is ultimately detrimental to wheat yield.

[0048] Comparing Comparative Examples 1 and 3, it can be seen that, compared to the application of tebuconazole spray alone in Comparative Example 1, the application of a tebuconazole-containing fertilizer product prepared by dissolving the compound fermentation liquid and tebuconazole water emulsion together in water in Comparative Example 3 resulted in little change in the rust control rate 14 days after the first application and 14 days after the second application. The wheat yield growth rate increased from 11.8% (Comparative Example 1) to 14.9% (Comparative Example 3). This indicates that directly mixing the compound fermentation liquid and tebuconazole water emulsion prepared by this invention has no significant impact on the rust control effect of tebuconazole and can significantly increase wheat yield.

[0049] Comparing with Example 1, it can be seen that by adding copper sulfate pentahydrate to the mixture of the compound fermentation broth and tebuconazole emulsion prepared in this invention, and dissolving them together in water to produce a tebuconazole-containing fertilizer product, the rust control rate 14 days after the first application and 14 days after the second application both increased slightly, and the wheat yield growth rate further increased. This indicates that adding copper sulfate pentahydrate to the mixture of the compound fermentation broth and tebuconazole emulsion prepared in this invention can produce a synergistic effect, synergistically improving the fungicidal efficacy and synergistically increasing wheat yield.

[0050] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A fertilizer product containing tebuconazole, characterized in that: It includes 26-30 mL of tebuconazole emulsion, 0.2-0.23 g of copper sulfate pentahydrate, 10-12 mL of compound fermentation broth, and 40 L of water; The concentration of tebuconazole in the tebuconazole water emulsion is 0.25 g / mL; The compound fermentation broth is prepared by fermentation of a mixture of corn flour, molasses, soybean meal, humic acid and water with compound bacteria, including Aspergillus niger and EM bacteria.

2. The tebuconazole-containing fertilizer product according to claim 1, characterized in that: The preparation method of the compound fermentation broth is as follows: A1. By weight, mix 30-35 parts corn flour, 8-10 parts molasses, 16-20 parts soybean meal, 5-6 parts humic acid and 30-35 parts water to obtain the base material; A2. Mix Aspergillus niger and EM bacteria at a mass ratio of 1:(0.8-1) to obtain a compound bacteria; A3. Adjust the pH of the substrate obtained in A1 to 5.0-5.5 with citric acid, sterilize and cool, inoculate with the compound bacteria obtained in A2, the mass of which is 1.5%-2% of the substrate mass, ferment, filter, and obtain the compound fermentation broth.

3. The tebuconazole-containing fertilizer product according to claim 1, characterized in that: In A3, the sterilization temperature is 120±2℃ and the sterilization time is 15-20min.

4. The tebuconazole-containing fertilizer product according to claim 1, characterized in that: In A3, the specific fermentation process is as follows: ferment at 28-30℃ with ventilation for 5-7 days, stirring 2-3 times a day.

5. The tebuconazole-containing fertilizer product according to claim 1, characterized in that: The EM bacteria contain lactic acid bacteria and yeast.

6. The tebuconazole-containing fertilizer product according to claim 1, characterized in that: The humic acid is fulvic acid.

7. The tebuconazole-containing fertilizer product according to claim 1, characterized in that: The tebuconazole-containing fertilizer product is used for wheat planting, and is sprayed twice consecutively, once at the wheat jointing stage and once at the heading stage, with a spraying amount of 40±2L / mu each time.

8. A method for preparing a tebuconazole-containing fertilizer product as described in any one of claims 1-7, characterized in that: First, add copper sulfate pentahydrate to water and stir. Then, add the compound fermentation liquid and continue stirring. Finally, add tebuconazole emulsion and continue stirring until well mixed to obtain the tebuconazole-containing fertilizer product.

9. The method for preparing the tebuconazole-containing fertilizer product according to claim 8, characterized in that: The stirring speed is 100-120 r / min.

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