Agricultural microorganism propagation device

Abstract

This invention relates to the field of microbial cultivation technology, specifically to an agricultural microbial propagation device, comprising a tank, which is double-layered; a heat exchange mechanism, the output and input ends of which are respectively connected to the top and bottom of the tank's interlayer; a drive assembly fixedly mounted on the tank; a stirring mechanism including a diaphragm located inside the tank, the output end of which is drively connected to the diaphragm for driving it to reciprocate up and down; and a guide tube fixedly mounted inside the tank and enclosing the diaphragm. This invention ensures that the diaphragm never contacts the inner wall of the guide tube during its reciprocating motion, avoiding the generation of solid abrasive particles. Simultaneously, it allows for moderate liquid leakage to reduce peak pressure, significantly lower than traditional stirring paddles, thus significantly reducing damage to the cell walls of the microorganisms. It overcomes the shortcomings of airlift reactors, such as weak mixing intensity, easy temperature stratification, and dissolved oxygen gradients, effectively improving temperature uniformity during the propagation process.

Description

Technical Field

[0001] This invention relates to the field of microbial cultivation technology, specifically to an agricultural microbial propagation device. Background Technology

[0002] In modern agricultural production, the application of microorganisms is becoming increasingly widespread. Through metabolic activities, they can provide nutrients for crops, improve soil structure, and inhibit harmful pathogens, which is of great significance for increasing crop yields, improving the quality of agricultural products, and realizing the development of green agriculture.

[0003] There are significant differences in process requirements between agricultural microbial propagation and medical microbial fermentation. Medical applications (such as vaccine and antibody production) require extremely high purity of the culture environment; even the smallest abrasive particles, leaks, or material leachates can lead to product defects. Therefore, their reactors typically employ high-cost magnetic stirring, disposable bioreactor bags, or fully enclosed airlift designs. Agricultural microbial propagation, on the other hand, primarily serves fields such as biofertilizers, biopesticides, feed additives, and environmental remediation. Its culture targets are mostly highly environmentally tolerant strains such as Bacillus, lactic acid bacteria, yeast, and actinomycetes. The final products are usually not used for human injection; therefore, the tolerance for trace amounts of solid particles in the culture medium is far higher than medical standards.

[0004] Existing agricultural microbial propagation equipment mostly uses traditional mechanical agitators or airlift mixing methods. While mechanical agitators offer high mixing efficiency, the high shear rate generated at the blade tips can easily damage the cell walls of microorganisms. Even at low rotational speeds, the blade edges can still achieve high circumferential speeds due to the proportionality of the outer linear velocity to the radius, significantly impacting filamentous fungi and some Gram-positive bacteria. Airlift reactors, while avoiding shear damage, suffer from weak mixing intensity and uneven mass and heat transfer. In the later stages of propagation, temperature stratification and dissolved oxygen gradients can easily occur, leading to inconsistent microbial metabolism and affecting propagation uniformity. Therefore, there is an urgent need for agricultural microbial propagation equipment that can achieve both high-efficiency mixing and uniform heat transfer under conditions of low shear, low pollution, and low cost. Summary of the Invention

[0005] To address the shortcomings of existing technologies, this invention provides an agricultural microbial propagation device, comprising: The tank body is double-layered; A heat exchange mechanism, wherein the output end and the input end of the heat exchange mechanism are respectively connected to the top and bottom of the tank body interlayer; A drive assembly, which is fixedly mounted on the tank body; A stirring mechanism, comprising a diaphragm located inside a tank, wherein the output end of a drive assembly is connected to the diaphragm for driving the diaphragm to reciprocate up and down; A flow guide tube is fixedly installed inside the tank and wraps around the diaphragm. The single-sided gap between the edge of the diaphragm and the inner wall of the flow guide tube is 5% to 10% of the radius of the diaphragm, and the single-sided gap between the outer wall of the flow guide tube and the inner wall of the tank is 8% to 18% of the inner diameter of the tank. An oxygen supply system used to oxygenate the liquid inside a tank.

[0006] As a preferred embodiment of the present invention, it also includes an oxygen supply system for oxygenating the liquid inside the tank.

[0007] As a preferred technical solution of the present invention: the driving component is a servo electric cylinder, and the stirring mechanism further includes a connecting member and a shaft fixedly connected to the output end of the driving component. The shaft is fixedly connected to the diaphragm through the connecting member.

[0008] As a preferred embodiment of the present invention: the connecting piece includes an upper mounting plate, a lower mounting plate and a bolt, the upper mounting plate is fixedly connected to the shaft, the lower mounting plate is movably connected to the shaft, the diaphragm has a mounting hole, the upper mounting plate and the lower mounting plate clamp the diaphragm, and the bolt passes through the mounting hole and locks the upper mounting plate and the lower mounting plate.

[0009] As a preferred embodiment of the present invention, the diaphragm is made of platinum vulcanized silicone rubber.

[0010] As a preferred technical solution of the present invention: the guide tube includes an upper expansion section and a lower expansion section, both of which are outwardly expanding, and the opposite ends of the upper expansion section and the lower expansion section are both set with arc-shaped chamfers.

[0011] As a preferred embodiment of the present invention: a spiral plate for forming a spiral flow channel is fixedly installed inside the tank jacket, the input end of the heat exchange mechanism is located at the bottom of the spiral flow channel, and the output end of the heat exchange mechanism is located at the top of the spiral flow channel.

[0012] As a preferred embodiment of the present invention: the heat exchange mechanism includes a fluid pump and a heater, the output end of the fluid pump is connected to the heater, the input end of the fluid pump is connected to a main outlet pipe connected to the tank jacket, and the output end of the heater is connected to a main inlet pipe connected to the tank jacket.

[0013] As a preferred embodiment of the present invention: the heat exchange mechanism further includes a spiral tube, which is sleeved on the outer wall of the guide tube, and the two ends of the spiral tube are respectively connected to the main liquid outlet pipe and the main liquid inlet pipe.

[0014] As a preferred embodiment of the present invention: the oxygen supply system includes an oxygenation fan and an aeration disc, the input end of the oxygenation fan is connected to an air filter, the output end of the oxygenation fan is connected to the aeration disc, and the aeration disc is located at the bottom of the inner cavity of the tank.

[0015] The present invention has the following beneficial effects: By setting the single-sided gap between the membrane edge and the inner wall of the guide tube to 5%~10% of the membrane radius, moderate liquid leakage is allowed during the reciprocating motion of the membrane to reduce peak pressure and control the shear rate within a low range, far lower than that of traditional impellers, significantly reducing damage to the bacterial cell walls. By setting the single-sided gap between the outer wall of the guide tube and the inner wall of the tank to 8%~18% of the inner diameter of the tank, a large-scale axial circulation is formed in coordination with the pushing direction of the membrane, shortening the mixing time to within 60 seconds, overcoming the defects of weak mixing intensity, easy temperature stratification, and dissolved oxygen gradient in airlift reactors. Combined with the heat exchange mechanism connected to the tank jacket for circulating heat exchange, the temperature uniformity during the propagation process is effectively improved. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the overall structure provided by the present invention.

[0017] Figure 2 This is a cross-sectional schematic diagram provided by the present invention.

[0018] Figure 3 This is a schematic diagram of the back side provided by the present invention.

[0019] Figure 4 This is a schematic diagram of the connector provided by the present invention.

[0020] Appendix Figures 1-4 The structures represented by each label are listed below: 1. Tank body; 11. Outer shell; 12. Inner shell; 13. Spiral plate; 14. Spiral flow channel; 15. Discharge pipe; 16. Discharge valve; 17. Feed plate; 18. Connecting pipe; 19. Connecting valve; 110. Top cover; 2. Drive components; 21. Bracket; 3. Flow guide tube; 31. Upper expansion section; 32. Lower expansion section; 33. Support rod; 4. Heat exchange mechanism; 41. Fluid pump; 42. Main outlet pipe; 43. Auxiliary outlet pipe; 44. Heater; 45. Spiral tube; 46. Main inlet pipe; 47. Auxiliary inlet pipe; 5. Mixing mechanism; 51. Shaft; 52. Connector; 521. Upper mounting plate; 522. Lower mounting plate; 523. Bolt; 53. Diaphragm; 531. Mounting hole; 6. Oxygen supply system; 61. Aeration blower; 62. Aeration pipe; 63. Aeration disc. Detailed Implementation

[0021] The principles and features of the present invention are described below. The embodiments given are only for explaining the present invention and are not intended to limit the scope of the present invention.

[0022] It should be noted that when a part or component is considered to be "connected to," "located on," or "assembled" to another part or component, it can be directly mounted on the other part or component, or it may be located in an intermediate part or component. The terms "left," "right," "upper," "lower," and similar expressions used in this document are for illustrative purposes only.

[0023] like Figures 1-4 As shown, this application designs an agricultural microbial propagation device, specifically including... Tank 1 is a double-layered tank. Tank 1 includes an outer shell 11 and an inner shell 12, with a sandwich formed between the inner wall of the outer shell 11 and the outer wall of the inner shell 12. The top and bottom of the outer shell 11 are fixedly connected to the top and bottom of the inner shell 12, respectively, to support the inner shell 12. Tank 1 also includes a discharge pipe 15 communicating with the bottom of the inner cavity of the inner shell 12. A discharge valve 16 is fixedly installed on the discharge pipe 15 to control its opening and closing. The discharge pipe 15 is used to discharge the microorganisms that have completed propagation within the inner shell 12. Tank 1 also includes a top cover 110 hinged to the outer shell 11. The top cover 110 has a feeding port, and a feed plate 17, matching the feeding port, is hinged to the top cover 110. The feed plate 17 is used to open / close the feeding port. A connecting pipe 18 that communicates with the inner cavity of the inner shell 12 is fixedly installed on the top cover 110. A connecting valve 19 is fixedly installed on the connecting pipe 18. The connecting pipe 18 is used to facilitate the addition of cleaning fluid, high-temperature sterilization steam, ozone, or sampling into the inner shell 12.

[0024] The heat exchange mechanism 4 has its output and input ends connected to the top and bottom of the interlayer of the tank 1, respectively. The heat exchange mechanism 4 is for liquid heat exchange; specifically, it inputs liquid heated to a specified temperature into the interlayer of the tank 1 to exchange heat with the propagation liquid inside the tank 1. The cooled liquid then returns to the heat exchange mechanism 4 for reheating, repeating the cycle until the propagation liquid in the tank 1 reaches the specified temperature. A temperature sensor (not shown in the figure) is fixedly installed inside the tank 1 to detect the temperature of the propagation liquid. This temperature sensor is electrically connected to a controller (not shown in the figure). The controller receives the actual temperature value from the temperature sensor and compares it with a preset target temperature value. When the actual temperature is lower than the target temperature, the controller activates the heat exchange mechanism 4 to heat the liquid. When the actual temperature approaches or reaches the target temperature, the controller reduces the heater power or makes the heater operate intermittently to maintain the tank temperature within the target value ±0.5℃. If the actual temperature exceeds the target temperature, the heat exchange mechanism 4 stops heating. The heat exchange mechanism 4 itself can adopt a structure known in the art, such as a constant temperature circulating water bath, an electric heating circulating system, or a steam-water heat exchange unit. The specific structure is all existing technology and will not be described in detail here.

[0025] Drive component 2 is fixedly installed on tank 1; specifically, a bracket 21 is fixedly installed on the top of tank 1, and drive component 2 is fixedly installed on bracket 21 to achieve a fixed connection with tank 1.

[0026] The stirring mechanism 5 includes a diaphragm 53 located inside the tank 1. The output end of the drive assembly 2 is connected to the diaphragm 53 for driving the diaphragm 53 to reciprocate up and down. The diaphragm 53 is coaxial with the tank 1 and is horizontally positioned. The output end of the drive assembly 2 drives the diaphragm 53 to reciprocate up and down, meaning the diaphragm 53 is always coaxial with the tank 1. Preferably, the diaphragm 53 has a gradually varying thickness, thicker at the center and thinner at the edges.

[0027] A flow guide tube 3 is fixedly installed inside the tank body 1 and encloses the diaphragm 53. The flow guide tube 3 is a thin-walled cylinder, and it is coaxial with the tank body 1 and the diaphragm 53. The single-sided gap between the edge of the diaphragm 53 and the inner wall of the flow guide tube 3 is 5% to 10% of the radius of the diaphragm 53, ensuring that the diaphragm 53 never contacts the inner wall of the flow guide tube 3 during movement, while allowing some of the diffusing liquid to pass through the edge of the diaphragm 53 and the inner wall of the flow guide tube 3 to reduce the shear rate without completely losing driving efficiency. The single-sided gap between the outer wall of the flow guide tube 3 and the inner wall of the tank body 1 is 8% to 18% of the inner diameter of the tank body 1, giving the liquid efficient flow capacity when flowing through it.

[0028] The aforementioned proportional range and design values ​​can be verified using conventional fluid simulation software (such as CFD), and those skilled in the art can determine the specific values ​​based on the teachings of this invention without any inventive effort. In practical applications, the proportional range can be further optimized based on factors such as the type of microorganism and the viscosity of the culture medium, all of which fall within the protection scope of this invention.

[0029] The verification process is as follows: Let the inner diameter D of the inner shell 12 be 500 mm, the radius of the diaphragm 53 be r, the single-sided gap between the edge of the diaphragm 53 and the inner wall of the guide tube 3 be δ1, and the single-sided gap between the outer wall of the guide tube 3 and the inner wall of the tank 1 be δ2. Let δ2 / D = 10%, then δ2 = 50 mm, and the outer diameter d1 of the guide tube 3 be 400 mm. Let the wall thickness of the guide tube 3 be 5 mm, then the inner diameter d2 of the guide tube 3 be 390 mm. Set the range of δ1 / r from 3% to 15% as an experimental variable, set the running speed of the diaphragm 53 to 0.5 m / s, and set the thickness of the diaphragm 53 to 10 mm.

[0030] The results obtained by running the simulation using fluid dynamics (CFD) software are shown in the table below: Table 1. The effect of δ1 / r on performance.

[0031]

[0032] In existing stirred microbial propagation equipment, the average shear rate increases with increasing stirring speed from approximately 400 s⁻¹. -1 The change is close to 2700 s -1 In particular, the local maximum shear rate in the blade tip region can reach 5000 s. -1 The above is sufficient to damage the bacterial cell wall. In Table 1, when δ1 / r is between 5% and 10%, the shear rate of membrane 53 is around 1100 s⁻¹. -1 up to 480 s -1 The shear rate is significantly lower than that in the blade tip region of stirred microbial propagation equipment. When δ1 / r is below 5%, the shear rate increases dramatically, even exceeding that of mechanically stirred paddles. When δ1 / r is above 10%, the propulsion efficiency decreases significantly, and the mixing time increases significantly. Therefore, 5% to 10% is the preferred range that balances low shear rate and mixing efficiency.

[0033] With other conditions remaining unchanged, δ1 / r was fixed at 8%, and the range of δ2 / D was set to 5% to 22%. The results were obtained by running the fluid dynamics simulation software (CFD) and are shown in Table 2. Table 2. Effect of δ2 / D on performance.

[0034]

[0035] The annular area refers to the annular cross-sectional area formed on the same horizontal plane between the outer wall of the guide tube 3 and the inner wall of the inner shell 12. The mixing time is optimal at δ2 / D=12%, approximately 48 seconds, and is better than the boundary value within the range of 8% to 18%. The annular area ratio corresponding to 8% to 18% is 29% to 45%. Within this range, the annular cross-sectional area is large enough to allow a sufficient proportion of liquid to participate in circulation within the annular gap, thereby shortening the mixing time and improving the mixing efficiency. Mixing time increases significantly outside this range, therefore 8% to 18% is the preferred range for improving mixing efficiency.

[0036] Example 1 like Figure 2 and Figure 4 As shown, the basic structure of the agricultural microbial propagation equipment in this embodiment is basically the same as that of the agricultural microbial propagation equipment in this application, except that the specific structure of the drive component 2 and the stirring mechanism 5 is different.

[0037] Drive component 2 is a servo electric cylinder. Compared to pneumatic oscillators, servo electric cylinders can precisely set the stroke, speed, acceleration, frequency, and motion waveform through a controller, while pneumatic oscillators are affected by the compressibility of gas, resulting in poor motion stability and difficulty in achieving complex waveforms.

[0038] The stirring mechanism 5 also includes a connector 52 and a shaft 51 fixedly connected to the output end of the drive assembly 2. A diaphragm 53 is fixedly connected to the shaft 51 via the connector 52 so that it moves up and down synchronously with the diaphragm 53. The shaft 51 extends vertically into the tank 1, and the relative position of the diaphragm 53 with the shaft 51 is fixed during operation.

[0039] The connector 52 includes an upper mounting plate 521, a lower mounting plate 522, and a bolt 523. The diaphragm 53 has a mounting hole 531. The upper mounting plate 521 and the lower mounting plate 522 hold the diaphragm 53. The bolt 523 passes through the mounting hole 531 and locks the upper mounting plate 521 and the lower mounting plate 522.

[0040] Specifically, the diaphragm 53 is sleeved on the shaft 51, the upper mounting plate 521 is fixedly connected to the shaft 51, and the lower mounting plate 522 is movably connected to the shaft 51. When the diaphragm 53 needs to be replaced, simply unscrew the bolt 523, remove the lower mounting plate 522 from the shaft 51, and the old diaphragm 53 can be removed and replaced with a new diaphragm 53.

[0041] Example 2 like Figure 4 As shown, the basic structure of the agricultural microbial propagation equipment in this embodiment is basically the same as that of the agricultural microbial propagation equipment of this application, the difference being the further explanation of the membrane 53.

[0042] The membrane 53 is made of platinum-cured silicone rubber. Platinum-cured silicone rubber has the characteristics of low solubility and non-toxicity to microorganisms. It does not produce byproducts such as acetophenone and tert-butanol from peroxide curing, has extremely low extract content, and will not contaminate the culture medium. It has been successfully applied in silicone rubber membrane bioreactors for treating microbial fermentation broth. Specifically, the hardness of the membrane 53 is selected in the range of Shore A 40~50, which combines good flexibility and resilience, and it needs to be replaced every 50-100 hours.

[0043] Example 3 like Figure 2 As shown, the basic structure of the agricultural microbial propagation equipment in this embodiment is basically the same as that of the agricultural microbial propagation equipment in this application, except for the specific structure of 3.

[0044] The guide tube 3 includes an upper expansion section 31 and a lower expansion section 32, both of which are outwardly flared, meaning that both ends of the guide tube 3 are "trumpet-shaped". The opposite ends of the upper expansion section 31 and the lower expansion section 32 are both designed with arc-shaped chamfers, so that the propagation liquid passes through both ends of the guide tube 3 more smoothly, avoiding damage to microorganisms.

[0045] Example 4 like Figure 2 Figure 3 As shown, the basic structure of the agricultural microbial propagation equipment in this embodiment is basically the same as that of the agricultural microbial propagation equipment in this application, except that the heat exchange mechanism 4 has a specific structure.

[0046] A spiral plate 13 for forming a spiral flow channel 14 is fixedly installed inside the jacket of the tank body 1. The input end of the heat exchange mechanism 4 is located at the bottom of the spiral flow channel 14, and the output end of the heat exchange mechanism 4 is located at the top of the spiral flow channel 14. This allows the heat exchange liquid in the jacket to flow through the jacket in a spiral path, ensuring uniform heat exchange and that all heat exchange liquids participate in the flow heat exchange. In other embodiments, the same flow path as the spiral flow channel 14 can be achieved by pre-embedding pipes in the jacket. Specifically, the pipes are pre-embedded in a spiral shape in the jacket, and the jacket is filled with heat exchange material, allowing the heat of the liquid in the pipes to be conducted to the inner shell 12 and the diffusion liquid inside through the heat exchange material.

[0047] The heat exchange mechanism 4 includes a fluid pump 41, a heater 44, and a condenser. The heater 44 and the condenser are connected in parallel and are connected to the output end of the fluid pump 41 through a three-way valve. The three-way valve controls the connection between the heater 44 or the condenser and the fluid pump 41, meaning that only one of the heater 44 and the condenser can be connected to the fluid pump 41 at any given time. The input end of the fluid pump 41 is connected to a main outlet pipe 42 that is connected to the interlayer of the tank 1. The output ends of the heater 44 and the condenser are connected to a main inlet pipe 46 that is connected to the interlayer of the tank 1.

[0048] The heat exchange mechanism 4 also includes a spiral tube 45, which is sleeved on the outer wall of the guide tube 3. Both ends of the spiral tube 45 are connected to the main outlet pipe 42 and the main inlet pipe 46, respectively. Specifically, the output end of the spiral tube 45 is connected to an auxiliary outlet pipe 43, which is connected to the main outlet pipe 42. The input end of the spiral tube 45 is connected to an auxiliary inlet pipe 47, which is connected to the main inlet pipe 46. That is, part of the heat exchange liquid enters the interior of the diffusion liquid through the spiral tube 45, exchanging heat with the central diffusion liquid, making the temperature of the diffusion liquid more uniform and the heat exchange rate faster.

[0049] Example 5 like Figure 2 As shown, the basic structure of the agricultural microbial propagation equipment in this embodiment is basically the same as that of the agricultural microbial propagation equipment in this application, except that the oxygen supply system 6 has a specific structure.

[0050] An oxygen supply system 6 is used to oxygenate the liquid inside tank 1. The oxygen supply system 6 can be an aerator. It is worth noting that, regardless of the form of the oxygen supply system 6, the air source of the oxygen supply system 6 should be filtered air or pure oxygen to prevent the air source input into the oxygen supply system 6 from contaminating microorganisms.

[0051] The oxygen supply system 6 includes an oxygenating blower 61 and an aeration disc 63. The input end of the oxygenating blower 61 is connected to an air filter, and the output end of the oxygenating blower 61 is connected to the aeration disc 63. Specifically, the output end of the oxygenating blower 61 is connected to an oxygenation pipe 62, which is connected to the aeration disc 63. The aeration disc 63 is located at the bottom of the inner cavity of the tank 1. The air filter has a built-in hydrophobic filter membrane with a precision of 0.22 μm, used to filter out dust, bacteria, and other particulate matter from the outside air, ensuring that the air entering the tank 1 is sterile, thereby preventing contamination or inhibition of the target microorganisms in the propagation solution due to airborne pollutants. The aeration disc 63 adopts a ring-shaped elastic microporous membrane structure. When the air supply stops, its own elastic recovery force causes the micropores to close naturally, effectively preventing the culture solution inside the tank from seeping back into the interior of the aeration disc 63 and the pipeline, avoiding blockage and cross-contamination caused by microorganisms colonizing and multiplying in the micropores. Meanwhile, a one-way valve (not shown in the figure) is installed on the oxygenation pipe 62 to further ensure the sterility of the pipeline system.

[0052] The usage process is as follows: The drive mechanism 2 drives the shaft 51 and diaphragm 53 to reciprocate up and down, causing the liquid in the tank 1 to form an axial circulation inside and outside the guide tube 3, achieving uniform mixing. The oxygenation system 6 can be selectively activated according to the bacterial species and the propagation stage. The oxygenation fan 62 sends sterile air purified by the air filter into the aeration disc 63. The elastic microporous membrane of the aeration disc 63 opens under water pressure, and the bubbles are distributed in the tank 1 with the circulation, providing dissolved oxygen for the microorganisms. The heat exchange system 4 can also be selectively activated according to the bacterial species and the propagation stage, maintaining a constant culture temperature through the spiral tube 45 on the outer wall of the guide tube 3 and the interlayer of the tank 1.

[0053] In this application, all devices and components whose structures are not described are commercially available devices or components.

[0054] In summary: By setting the single-sided gap between the edge of the diaphragm 53 and the inner wall of the guide tube 3 to 5%~10% of the radius of the diaphragm 53, moderate liquid leakage is allowed during the reciprocating motion of the diaphragm 53 to reduce peak pressure and control the shear rate within a low range, far lower than that of traditional impellers, significantly reducing damage to the cell walls of the bacteria; by setting the single-sided gap between the outer wall of the guide tube 3 and the inner wall of the tank 1 to 8%~18% of the inner diameter of the tank 1, a large-scale axial circulation is formed in coordination with the pushing direction of the diaphragm 53, shortening the mixing time to within 60 seconds, overcoming the defects of weak mixing intensity, easy temperature stratification and dissolved oxygen gradient in airlift reactors; and by connecting the heat exchange mechanism 4 with the jacket of the tank 1 for circulating heat exchange, the temperature uniformity during the propagation process is effectively improved.

[0055] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention in any way. Those skilled in the art can readily implement the present invention based on the accompanying drawings and the description above. However, any modifications, alterations, or variations made by those skilled in the art without departing from the scope of the present invention, using the disclosed technical content, are equivalent embodiments of the present invention. Furthermore, any modifications, alterations, or variations made to the above embodiments based on the essential technology of the present invention are still within the protection scope of the present invention.

Claims

1. An agricultural microbial propagation device, characterized in that: include: Tank body (1), wherein the tank body (1) is double-layered; The heat exchange mechanism (4) has its output end and input end connected to the top and bottom of the interlayer of the tank body (1), respectively. Drive assembly (2), which is fixedly installed on tank body (1); The stirring mechanism (5) includes a diaphragm (53) located inside the tank (1), and the output end of the driving component (2) is connected to the diaphragm (53) for driving the diaphragm (53) to move up and down reciprocally. A flow guide tube (3) is fixedly installed inside the tank (1) and wraps the diaphragm (53). The single-sided gap between the edge of the diaphragm (53) and the inner wall of the flow guide tube (3) is 5% to 10% of the radius of the diaphragm (53). The single-sided gap between the outer wall of the flow guide tube (3) and the inner wall of the tank (1) is 8% to 18% of the inner diameter of the tank (1).

2. The agricultural microbial propagation equipment according to claim 1, characterized in that: It also includes an oxygen supply system (6) for oxygenating the liquid inside the tank (1).

3. The agricultural microbial propagation equipment according to claim 2, characterized in that: The drive assembly (2) is a servo electric cylinder, and the stirring mechanism (5) also includes a connector (52) and a shaft (51) fixedly connected to the output end of the drive assembly (2). The shaft (51) is fixedly connected to the diaphragm (53) through the connector (52).

4. The agricultural microbial propagation equipment according to claim 3, characterized in that: The connector (52) includes an upper mounting plate (521), a lower mounting plate (522), and a bolt (523). The upper mounting plate (521) is fixedly connected to the shaft (51), and the lower mounting plate (522) is movably connected to the shaft (51). The diaphragm (53) has a mounting hole (531). The upper mounting plate (521) and the lower mounting plate (522) clamp the diaphragm (53). The bolt (523) passes through the mounting hole (531) and locks the upper mounting plate (521) and the lower mounting plate (522).

5. The agricultural microbial propagation equipment according to claim 1, characterized in that: The diaphragm (53) is made of platinum vulcanized silicone rubber.

6. The agricultural microbial propagation equipment according to claim 1, characterized in that: The guide tube (3) includes an upper expansion section (31) and a lower expansion section (32). Both the upper expansion section (31) and the lower expansion section (32) are outwardly flared, and the opposite ends of the upper expansion section (31) and the lower expansion section (32) are both set with arc-shaped chamfers.

7. The agricultural microbial propagation equipment according to claim 1, characterized in that: The tank body (1) has a spiral plate (13) fixedly installed in the interlayer to form a spiral flow channel (14). The input end of the heat exchange mechanism (4) is located at the bottom of the spiral flow channel (14), and the output end of the heat exchange mechanism (4) is located at the top of the spiral flow channel (14).

8. The agricultural microbial propagation equipment according to claim 7, characterized in that: The heat exchange mechanism (4) includes a fluid pump (41), a heater (44) and a condenser. The heater (44) and the condenser are connected in parallel and connected to the output end of the fluid pump (41) through a three-way valve. The three-way valve controls the connection between the heater (44) or the condenser and the fluid pump (41). The input end of the fluid pump (41) is connected to a main outlet pipe (42) that is connected to the interlayer of the tank body (1). The output ends of the heater (44) and the condenser are connected to a main inlet pipe (46) that is connected to the interlayer of the tank body (1).

9. The agricultural microbial propagation equipment according to claim 8, characterized in that: The heat exchange mechanism (4) also includes a spiral tube (45), which is sleeved on the outer wall of the guide tube (3). The two ends of the spiral tube (45) are connected to the main liquid outlet pipe (42) and the main liquid inlet pipe (46), respectively.

10. An agricultural microbial propagation device according to claim 2, characterized in that: The oxygen supply system (6) includes an oxygenation fan (61) and an aeration disc (63). The input end of the oxygenation fan (61) is connected to an air filter, and the output end of the oxygenation fan (61) is connected to the aeration disc (63). The aeration disc (63) is located at the bottom of the inner cavity of the tank (1).

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