A plant-based protein extraction system
Patent Information
- Application Number
- CN202610716410.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-05-22
- Publication Date
- 2026-08-18
AI Technical Summary
[0002]植物中含有大量的蛋白,例如谷物类、豆类植物中均含有蛋白,并且目前也有从上述植物中提取蛋白的工艺,例如授权公告号为CN101695334B的发明专利公开了一种用大米提取大米蛋白的方法,该方法主要包括浸泡磨浆、酶解、干燥、粉碎、包装;该方法具有以下缺点:1、该工艺中,通过离心后的液相直接送至淀粉糖的生产,然后,液相中还是会存留一些蛋白,因此蛋白无法彻底提取;2、该工艺中提取蛋白的纯度较低,酶解后直接灭酶后就简单的洗涤、离心干燥,蛋白的纯度难以保证
[0035] After adopting the above technical solution, the effects of the present invention are as follows: Compared with the existing solutions, the extraction system has at least the following advantages: 1. The extraction system uses a primary enzymatic hydrolysis tank and a secondary enzymatic hydrolysis tank for secondary enzymatic hydrolysis. Using two enzymatic hydrolysis methods allows for more efficient decomposition of the substrate, converting it into small molecule peptides and amino acids; 2. The solid phase outlet of the first centrifuge device is returned to the primary enzymatic hydrolysis tank, allowing the separated solid phase material to undergo secondary enzymatic hydrolysis, further extracting the effective components from the plant and reducing waste; 3. The extraction system uses a coupled membrane filtration device, which can efficiently and accurately remove small molecule impurities and purify and concentrate the target protein components. This coupled membrane filtration device performs two core tasks: first, removing small molecule impurities (such as salts, pigments, etc.), as residual impurities will affect the purity and quality of the product; second, concentrating the target protein components to increase the protein content and bioactivity of the product; 4. The extraction system uses a chromatography system, employing ion exchange chromatography technology to separate and purify plant proteins, thereby improving protein purity.
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Figure CN122587864A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to a plant protein extraction system for extracting plant proteins from plants. Background Technology
[0002] Plants contain a large amount of protein, such as grains and legumes. There are currently processes for extracting protein from these plants. For example, the invention patent with authorization announcement number CN101695334B discloses a method for extracting rice protein from rice. This method mainly includes soaking and grinding, enzymatic hydrolysis, drying, pulverizing, and packaging. However, this method has the following drawbacks: 1. In this process, the liquid phase after centrifugation is directly sent to the starch sugar production process, and some protein will still remain in the liquid phase, thus the protein cannot be completely extracted; 2. The purity of the protein extracted by this process is low. After enzymatic hydrolysis, the enzyme is directly inactivated, followed by simple washing, centrifugation, and drying, making it difficult to guarantee the purity of the protein. Summary of the Invention
[0003] The technical problem to be solved by the present invention is to provide a plant protein extraction system that can extract proteins with higher purity from plants.
[0004] To solve the above-mentioned technical problems, the technical solution of the present invention is: a plant protein extraction system, comprising:
[0005] Vacuum feeders are used to feed protein-containing plant materials.
[0006] The cleaning machine, located downstream of the vacuum feeder, is used to clean the materials.
[0007] A soaking tank, located downstream of the washing machine, is used to receive the material after it has been washed by the washing machine and soak it. The inlet of the soaking tank is connected to a pure water supply device.
[0008] The grinding device is located downstream of the soaking tank and is used to receive the soaked material for grinding and crushing.
[0009] A receiving and mixing tank is located downstream of the grinding device and is used to receive the ground and crushed material, mix it into a slurry, and temporarily store it. The water inlet and acid / alkali outlet of the receiving and mixing tank are connected to the pure water supply device and the acid / alkali supply device, respectively.
[0010] A primary enzymatic hydrolysis tank for enzymatically hydrolyzing material slurry is located downstream of the receiving and conditioning tank and connected to the discharge port of the receiving and conditioning tank. The water inlet and acid / alkali outlet of the primary enzymatic hydrolysis tank are connected to a pure water supply device and an acid / alkali supply device, respectively.
[0011] A first centrifugal device for performing a single centrifugal separation of materials is located downstream of a primary enzymatic hydrolysis tank and connected to the discharge port of the primary enzymatic hydrolysis tank. The solid phase outlet of the first centrifugal device is reflux-connected to the feed inlet of the primary enzymatic hydrolysis tank.
[0012] A secondary enzymatic hydrolysis tank for enzymatically hydrolyzing material slurry is located downstream of the first centrifuge device. The feed inlet of the secondary enzymatic hydrolysis tank is connected to the liquid phase outlet of the first centrifuge device. The water inlet and acid / alkali inlet of the secondary enzymatic hydrolysis tank are connected to a pure water supply device and an acid / alkali supply device, respectively.
[0013] A second centrifugal device for secondary centrifugal separation of materials is located downstream of the secondary enzymatic hydrolysis tank and connected to the discharge port of the secondary enzymatic hydrolysis tank; the liquid phase outlet of the second centrifugal device is connected to the downstream collection tank.
[0014] The protein reaction vessel is located downstream of the second centrifuge. The inlet of the protein reaction vessel is connected to the solid phase outlet of the second centrifuge. The water inlet and acid / alkali outlet of the protein reaction vessel are connected to the pure water supply device and the acid / alkali supply device, respectively.
[0015] The vacuum transfer tank, located downstream of the protein reactor, is used to receive and buffer the materials reacted in the protein reactor.
[0016] A third centrifuge device is located downstream of the vacuum transfer tank, and the liquid phase outlet of the third centrifuge device is connected to the supernatant storage tank.
[0017] The decolorizing stirring tank is located downstream of the third centrifuge device. The decolorizing stirring tank is connected to the solid phase outlet of the third centrifuge device. The water inlet and acid / alkali outlet of the decolorizing stirring tank are connected to the pure water supply device and the acid / alkali supply device, respectively.
[0018] A coupled membrane filter device is installed downstream of the supernatant storage tank. The coupled membrane filter device is connected to the liquid phase outlet of the third centrifuge device and is used to filter the supernatant after centrifugation in the third centrifuge device. The coupled membrane filter device is connected to the pure water supply system.
[0019] An intermediate storage tank is located downstream of the decolorizing mixing tank, with its inlet connected to the outlet of the decolorizing mixing tank; the acid / alkali outlet of the intermediate storage tank is connected to an acid / alkali supply device.
[0020] A chromatography system is installed downstream of the intermediate storage tank and is connected to the outlet of the intermediate storage tank.
[0021] A transfer tank is located downstream of the chromatography system and is connected to the outlet of the chromatography system; the acid and alkali outlet of the transfer tank is connected to an acid and alkali supply device.
[0022] A sterilization device is installed downstream of the transfer tank, and the sterilization device is connected to the discharge port of the transfer tank for sterilizing the material.
[0023] A sterile storage tank is located downstream of the sterilization device. The sterile storage tank is connected to the discharge port of the sterilization device, and the acid and alkali outlet of the sterile storage tank is connected to an acid and alkali supply device.
[0024] A dual-effect concentration system is installed downstream of the sterile storage tank. The inlet of the dual-effect concentration system is connected to the outlet of the sterile storage tank and the coupling membrane filter device, and is used to concentrate the material.
[0025] A concentrated liquid stirring tank is located downstream of the double-effect concentration system. The concentrated liquid stirring tank is connected to the discharge port of the double-effect concentration system, and the acid and alkali liquid outlet of the concentrated liquid stirring tank is connected to the acid and alkali liquid supply device.
[0026] A spray dryer is installed downstream of the concentrated liquid mixing tank. The spray dryer is connected to the discharge port of the concentrated liquid mixing tank and is used to spray dry the concentrated liquid to produce protein powder.
[0027] The ultrafine pulverizer, located downstream of the powder dryer, is used to pulverize and grind protein powder.
[0028] The hopper located downstream of the ultrafine pulverizer is used to collect the pulverized and ground protein material.
[0029] As a preferred embodiment, the grinding device includes a primary colloid mill and a secondary colloid mill connected in series. The feed inlet of the primary colloid mill is equipped with a feed mixing tank, which is connected to the outlet of the soaking tank via an elevator. The outlet of the secondary colloid mill is connected to the receiving and mixing cylinder.
[0030] As a preferred embodiment, the first centrifugation device includes a horizontal centrifuge located upstream and a disc centrifuge located downstream. The inlet of the horizontal centrifuge is connected to the outlet of the primary enzymatic hydrolysis tank, and the inlet of the disc centrifuge is connected to the liquid phase outlet of the horizontal centrifuge. The liquid phase outlet of the disc centrifuge is connected to the inlet of the downstream secondary enzymatic hydrolysis tank. The solid phase outlets of the horizontal centrifuge and the disc centrifuge are refluxed and connected to the inlet of the primary enzymatic hydrolysis tank.
[0031] As a preferred embodiment, the outlet of the collection tank is connected to the inlet of the feeding mixing tank.
[0032] As a preferred embodiment, the outlet of the collection tank is connected to the inlet of the primary enzymatic hydrolysis tank.
[0033] As a preferred embodiment, the coupled membrane filtration device includes an upstream ceramic membrane filtration device and a downstream nanofiltration membrane filtration device, wherein the ceramic membrane filtration device has a molecular weight cutoff of 50-100 kDa, and the nanofiltration membrane filtration device has a molecular weight cutoff of 500-530 kDa.
[0034] As a preferred embodiment, the liquid phase outlet of the second centrifuge is also connected to a stationary tank.
[0035] After adopting the above technical solution, the effects of the present invention are as follows: Compared with the existing solutions, the extraction system has at least the following advantages: 1. The extraction system uses a primary enzymatic hydrolysis tank and a secondary enzymatic hydrolysis tank for secondary enzymatic hydrolysis. Using two enzymatic hydrolysis methods allows for more efficient decomposition of the substrate, converting it into small molecule peptides and amino acids; 2. The solid phase outlet of the first centrifuge device is returned to the primary enzymatic hydrolysis tank, allowing the separated solid phase material to undergo secondary enzymatic hydrolysis, further extracting the effective components from the plant and reducing waste; 3. The extraction system uses a coupled membrane filtration device, which can efficiently and accurately remove small molecule impurities and purify and concentrate the target protein components. This coupled membrane filtration device performs two core tasks: first, removing small molecule impurities (such as salts, pigments, etc.), as residual impurities will affect the purity and quality of the product; second, concentrating the target protein components to increase the protein content and bioactivity of the product; 4. The extraction system uses a chromatography system, employing ion exchange chromatography technology to separate and purify plant proteins, thereby improving protein purity.
[0036] Furthermore, since the first centrifugation device includes an upstream horizontal centrifuge and a downstream disc centrifuge, the inlet of the horizontal centrifuge is connected to the outlet of the primary enzymatic hydrolysis tank, and the inlet of the disc centrifuge is connected to the liquid phase outlet of the horizontal centrifuge; the liquid phase outlet of the disc centrifuge is connected to the inlet of the downstream secondary enzymatic hydrolysis tank; the solid phase outlets of the horizontal centrifuge and the disc centrifuge are refluxed to the inlet of the primary enzymatic hydrolysis tank, wherein the horizontal centrifuge and the disc centrifuge are used for two centrifugation separations, and a secondary fine separation process is implemented.
[0037] Furthermore, the outlet of the collection tank is connected to the inlet of the feeding mixing tank. The outlet of the collection tank is also connected to the inlet of the primary enzymatic hydrolysis tank. Therefore, the material in the collection tank can be returned to the primary enzymatic hydrolysis tank or the feeding mixing tank for further enzymatic hydrolysis or re-grinding.
[0038] Furthermore, since the coupled membrane filtration device includes an upstream ceramic membrane filtration device and a downstream nanofiltration membrane filtration device, the ceramic membrane filtration device has a molecular weight cutoff of 50-100 kDa, and the nanofiltration membrane filtration device has a molecular weight cutoff of 500-530 kDa. This filtration device can filter materials better, and the extraction rate of protein from plants is higher. Attached Figure Description
[0039] The present invention will be further described below with reference to the accompanying drawings and embodiments.
[0040] Figure 1 This is a schematic diagram of the structure of the first part of an embodiment of the present invention;
[0041] Figure 2 This is a schematic diagram of the structure of the second part of an embodiment of the present invention;
[0042] Figure 3 This is a schematic diagram of the third part of an embodiment of the present invention;
[0043] Figure 4 This is a schematic diagram of the fourth part of an embodiment of the present invention;
[0044] In the attached diagram: 1. Vacuum feeder; 2. Pure water supply device; 3. Acid / alkali solution supply device; 4. Washing machine; 5. Soaking tank; 6. Primary colloid mill; 7. Secondary colloid mill; 8. Receiving and mixing tank; 9. Air compressor system; 10. Primary enzymatic hydrolysis device; 11. First centrifuge; 11. Horizontal centrifuge; 11. Disc centrifuge; 13. Secondary enzymatic hydrolysis tank; 14. Hot water supply system; 15. Reflux pipe; 16. Second centrifuge; 17. Protein reaction tank. ; 18. Vacuum transfer tank; 19. Third centrifuge unit; 20. Supernatant storage tank; 21. Ceramic membrane filtration device; 22. Nanofiltration membrane filtration device; 23. Decolorization mixing tank; 24. Collection tank; 25. Stagnation tank; 26. Intermediate storage tank; 27. Chromatography system; 28. Transfer tank; 29. Sterilization device; 30. Aseptic storage tank; 31. Double-effect concentration system; 32. Concentrate mixing tank; 33. Powder spray dryer; 34. Ultrafine pulverizer; 35. Silo. Detailed Implementation
[0045] The present invention will be further described in detail below through specific embodiments.
[0046] like Figures 1 to 4 As shown, this invention discloses a plant protein extraction system, comprising:
[0047] Vacuum feeder 1 is used to feed protein-containing plant materials;
[0048] The washing machine 3, located downstream of the vacuum feeder 1, is used to wash the material. The vacuum feeder 1 and the washing machine 3 are connected by a lifting conveyor. The washing machine 3 is connected to the pure water supply device 2, using pure water to wash the plant material, which can be soybeans or grains.
[0049] Soaking tank 5, located downstream of washing machine 3, is used to receive and soak the material washed by washing machine 3. The inlet of soaking tank 5 is connected to pure water supply device 2; wherein pure water supply device 2 can be a pure water filtration device with RO reverse osmosis membrane. When plant material enters soaking tank 5, it absorbs water and swells, thus facilitating subsequent grinding and crushing.
[0050] A grinding device, located downstream of the soaking tank 5, is used to receive the soaked material and grind it. The grinding device includes a primary colloid mill 6 and a secondary colloid mill 7, connected in series. The feed inlet of the primary colloid mill 6 is connected to a feeding mixing tank, which is connected to the outlet of the soaking tank 5 via an elevator. The outlet of the secondary colloid mill 7 is connected to the receiving and adjusting slurry tank 8. The grinding device is also connected to a pure water supply device 2 and an acid / alkali supply device 3, allowing pure water or acid / alkali solutions to be introduced during the grinding process for rinsing the ground material and facilitating slurry flow.
[0051] The receiving and mixing cylinder 8 is located downstream of the grinding device and is used to receive the ground and crushed material, mix it into a slurry, and temporarily store it. The water inlet and acid / alkali outlet of the receiving and mixing cylinder 8 are connected to the pure water supply device 2 and the acid / alkali supply device 3, respectively.
[0052] A primary enzymatic hydrolysis tank 10 for enzymatically hydrolyzing material slurry is located downstream of the receiving and adjusting tank 8 and connected to the discharge port of the receiving and adjusting tank 8. The water inlet and acid / alkali outlet of the primary enzymatic hydrolysis tank 10 are connected to the pure water supply device 2 and the acid / alkali supply device 3, respectively. There are two primary enzymatic hydrolysis tanks 10, which are arranged in series.
[0053] like Figure 1 and Figure 2 As shown, Figure 1 and Figure 2 The pipes in the diagram are interconnected, but the same pipes have been disconnected and labeled with the same letters for better illustration.
[0054] Among them, a1 is the acid and alkali solution pipeline; b1 is the pure water pipeline; c1 is the material pipeline; d1 is the acid and alkali solution pipeline, used for acid and alkali solution reflux; e1 is the steam pipeline, used to introduce steam to facilitate heating of the subsequent tanks; f1 is the sewage discharge pipeline; g1 is the condensate pipeline.
[0055] The acid and alkali supply device 3 mainly includes an acid tank, an alkali tank, and a hot water tank. The acid tank and alkali tank are connected to a concentrated acid tank and a concentrated alkali tank, respectively. In this way, the acid tank, alkali tank, and hot water tank are used to prepare acid or alkali solutions of corresponding concentrations. Then, the prepared acid or alkali solutions are exchanged with steam and condensate through a heat exchanger, so that the temperature of the acid or alkali solutions after heat exchange in the acid and alkali supply device 3 is controllable.
[0056] The entire extraction system also includes an air compressor system 9, which is used to provide a vacuum.
[0057] A first centrifugal device 11 for centrifuging materials in a single operation is located downstream of a primary enzymatic hydrolysis tank 10 and is connected to the discharge port of the primary enzymatic hydrolysis tank 10. The solid phase outlet of the first centrifugal device 11 is reflux-connected to the feed inlet of the primary enzymatic hydrolysis tank 10.
[0058] The first centrifuge device 11 includes a horizontal centrifuge 111 located upstream and a butterfly centrifuge 112 located downstream. The feed inlet of the horizontal centrifuge 111 is connected to the discharge outlet of the primary enzymatic hydrolysis tank 10, and the feed inlet of the butterfly centrifuge 112 is connected to the liquid phase outlet of the horizontal centrifuge 111. The liquid phase outlet of the butterfly centrifuge 112 is connected to the feed inlet of the secondary enzymatic hydrolysis tank 13 downstream. The solid phase outlets of the horizontal centrifuge 111 and the butterfly centrifuge 112 are connected to the feed inlet of the primary enzymatic hydrolysis tank 10 via a reflux pipe 15.
[0059] A secondary enzymatic hydrolysis tank 13 for enzymatically hydrolyzing the material slurry is located downstream of the first centrifuge device 11. The inlet of the secondary enzymatic hydrolysis tank 13 is connected to the liquid phase outlet of the first centrifuge device 11. The water inlet and acid / alkali inlet of the secondary enzymatic hydrolysis tank 13 are connected to the pure water supply device 2 and the acid / alkali supply device 3, respectively. Similarly, there are two secondary enzymatic hydrolysis tanks 13 connected sequentially. The upstream primary enzymatic hydrolysis tank 10 is for preliminary enzymatic hydrolysis, and the downstream secondary enzymatic hydrolysis tank 13 is for deep enzymatic hydrolysis.
[0060] like Figure 2 As shown, h1, i1, j1, k1, q1, and r1 are all hot circulating water pipes; the hot circulating water pipes are connected to the hot water supply system 14 and are used to heat the jackets of the primary enzymatic hydrolysis tank 10 and the secondary enzymatic hydrolysis tank 13, which facilitates cooling and temperature control of the tank body; while l1 is a material pipe; m1 is a pure water pipe; n1 is a steam pipe; and o1 and p1 are acid and alkali solution pipes.
[0061] A second centrifuge device 16 for secondary centrifugal separation of materials is located downstream of the secondary enzymatic hydrolysis tank 13 and connected to the discharge port of the secondary enzymatic hydrolysis tank 13; the liquid phase outlet of the second centrifuge device 16 is connected to the downstream collection tank 24; such as Figure 3 As shown, Figure 3The material in the upper part flows from right to left; while the material in the lower part flows from left to right.
[0062] Similarly, the second centrifuge device 16 also includes an upstream horizontal centrifuge 111 and a downstream disc centrifuge 112. The outlet of the collection tank 24 is connected to the inlet of the feeding mixing tank. The outlet of the collection tank 24 is also connected to the inlet of the primary enzymatic hydrolysis tank 10.
[0063] The liquid phase outlet of the second centrifuge device 16 is also connected to the settling tank 25. There are multiple settling tanks 25. During normal production, the liquid phase after centrifugation by the second centrifuge is sent to the collection tank 24. Then, the material in the collection tank 24 is sent to the grinding device or the primary enzymatic hydrolysis tank 10, so that it can re-enter the subsequent extraction process.
[0064] The protein reaction vessel 17 is located downstream of the second centrifuge 16. The inlet of the protein reaction vessel 17 is connected to the solid phase outlet of the second centrifuge 16. The water inlet and acid / alkali outlet of the protein reaction vessel 17 are connected to the pure water supply device 2 and the acid / alkali supply device 3, respectively.
[0065] The protein reaction vessel 17 is the stage for terminating the enzymatic hydrolysis reaction. It is designed to gradually stop the enzymatic hydrolysis reaction at the end of the reaction to prevent excessive hydrolysis from adversely affecting product quality. Simultaneously, the enzymatic hydrolysis products are further stabilized by adjusting the pH value and employing other chemical methods to ensure their stability in subsequent processing steps.
[0066] A vacuum transfer tank 18 is located downstream of the protein reaction vessel 17 to receive and buffer the materials reacted in the protein reaction vessel 17; a third centrifuge device 19 is located downstream of the vacuum transfer tank 18, and the liquid phase outlet of the third centrifuge device 19 is connected to the supernatant storage tank 20; the third centrifuge device 19 is preferably a disc centrifuge 112.
[0067] The decolorizing stirring tank 23 is located downstream of the third centrifuge device 19. The decolorizing stirring tank 23 is connected to the solid phase outlet of the third centrifuge device 19. The water inlet and acid / alkali outlet of the decolorizing stirring tank 23 are connected to the pure water supply device 2 and the acid / alkali supply device 3, respectively.
[0068] A coupling membrane filter is installed downstream of the supernatant storage tank 20. The coupling membrane filter is connected to the liquid phase outlet of the third centrifuge device 19 and is used to filter the supernatant after centrifugation in the third centrifuge device. The coupling membrane filter is connected to the pure water supply system.
[0069] The coupled membrane filtration device includes an upstream ceramic membrane filtration device 21 and a downstream nanofiltration membrane filtration device 22. The ceramic membrane filtration device 21 has a molecular weight cutoff of 50-100 kDa, and the nanofiltration membrane filtration device 22 has a molecular weight cutoff of 500-530 kDa.
[0070] Among them, a filter screen can also be installed at the material inlet of the ceramic membrane filter device 21 for coarse filtration.
[0071] An intermediate storage tank 26 is located downstream of the decolorizing mixing tank 23. The inlet of the intermediate storage tank 26 is connected to the outlet of the decolorizing mixing tank 23. The acid and alkali outlet of the intermediate storage tank 26 is connected to the acid and alkali supply device 3.
[0072] like Figure 3 and Figure 4 As shown in the attached diagram, a2 is a pure water pipeline; b2, d2, k2, and l2 are acid and alkali solution pipelines; c2, m2, and p2 are hot circulating water pipelines; e2 and j2 are steam pipelines; f2, i2, and o2 are material pipelines; g2 and q2 are sewage discharge pipelines; and h2 and r2 are condensate pipelines.
[0073] A chromatography system 27 is located downstream of the intermediate storage tank 26 and is connected to the outlet of the intermediate storage tank 26. The chromatography system 27 can be a conventional chromatography system and uses a chromatography column for separation and purification.
[0074] A transfer tank 28 is located downstream of the chromatography system 27 and is connected to the outlet of the chromatography system 27; the acid and alkali outlet of the transfer tank 28 is connected to the acid and alkali supply device 3.
[0075] A sterilization device 29 is installed downstream of the transfer tank 28. The sterilization device 29 is connected to the discharge port of the transfer tank 28 and is used to sterilize the material. The sterilization device 29 is a UHT sterilization device 29.
[0076] The sterile storage tank 30 is located downstream of the sterilization device 29. The sterile storage tank 30 is connected to the discharge port of the sterilization device 29, and the acid and alkali liquid outlet of the sterile storage tank 30 is connected to the acid and alkali liquid supply device 3.
[0077] The dual-effect concentration system 31 is located downstream of the sterile storage tank 30. The inlet of the dual-effect concentration system 31 is connected to the outlet of the sterile storage tank 30 and the coupling membrane filter device, and is used to concentrate the material.
[0078] The concentrated liquid stirring tank 32 is located downstream of the double-effect concentration system 31. The concentrated liquid stirring tank 32 is connected to the discharge port of the double-effect concentration system 31, and the acid and alkali liquid outlet of the concentrated liquid stirring tank 32 is connected to the acid and alkali liquid supply device 3.
[0079] A spray dryer 33 is located downstream of the concentrated liquid mixing tank 32. The spray dryer 33 is connected to the discharge port of the concentrated liquid mixing tank 32 and is used to spray dry the concentrated liquid to produce protein powder.
[0080] The ultrafine pulverizer 34, located downstream of the powder dryer 33, is used to pulverize and grind the protein powder; the hopper 35, located downstream of the ultrafine pulverizer 34, is used to collect the pulverized and ground protein material.
[0081] The above-described embodiments are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Any modifications and alterations made to the technical solutions of the present invention without departing from the spirit of the present invention shall fall within the protection scope defined by the claims of the present invention.
Claims
1. A plant protein extraction system, characterized in that: include: Vacuum feeders are used to feed protein-containing plant materials. The cleaning machine, located downstream of the vacuum feeder, is used to clean the materials. A soaking tank, located downstream of the washing machine, is used to receive the material after it has been washed by the washing machine and soak it. The inlet of the soaking tank is connected to a pure water supply device. The grinding device is located downstream of the soaking tank and is used to receive the soaked material for grinding and crushing. A receiving and mixing tank is located downstream of the grinding device and is used to receive the ground and crushed material, mix it into a slurry, and temporarily store it. The water inlet and acid / alkali outlet of the receiving and mixing tank are connected to the pure water supply device and the acid / alkali supply device, respectively. A primary enzymatic hydrolysis tank for enzymatically hydrolyzing material slurry is located downstream of the receiving and conditioning tank and connected to the discharge port of the receiving and conditioning tank. The water inlet and acid / alkali outlet of the primary enzymatic hydrolysis tank are connected to a pure water supply device and an acid / alkali supply device, respectively. A first centrifugal device for performing a single centrifugal separation of materials is located downstream of a primary enzymatic hydrolysis tank and connected to the discharge port of the primary enzymatic hydrolysis tank. The solid phase outlet of the first centrifugal device is reflux-connected to the feed inlet of the primary enzymatic hydrolysis tank. A secondary enzymatic hydrolysis tank for enzymatically hydrolyzing material slurry is located downstream of the first centrifuge device. The feed inlet of the secondary enzymatic hydrolysis tank is connected to the liquid phase outlet of the first centrifuge device. The water inlet and acid / alkali inlet of the secondary enzymatic hydrolysis tank are connected to a pure water supply device and an acid / alkali supply device, respectively. A second centrifugal device for secondary centrifugal separation of materials is located downstream of the secondary enzymatic hydrolysis tank and connected to the discharge port of the secondary enzymatic hydrolysis tank; the liquid phase outlet of the second centrifugal device is connected to the downstream collection tank. The protein reaction vessel is located downstream of the second centrifuge. The inlet of the protein reaction vessel is connected to the solid phase outlet of the second centrifuge. The water inlet and acid / alkali outlet of the protein reaction vessel are connected to the pure water supply device and the acid / alkali supply device, respectively. The vacuum transfer tank, located downstream of the protein reactor, is used to receive and buffer the materials reacted in the protein reactor. A third centrifuge device is located downstream of the vacuum transfer tank, and the liquid phase outlet of the third centrifuge device is connected to the supernatant storage tank. The decolorizing stirring tank is located downstream of the third centrifuge device. The decolorizing stirring tank is connected to the solid phase outlet of the third centrifuge device. The water inlet and acid / alkali outlet of the decolorizing stirring tank are connected to the pure water supply device and the acid / alkali supply device, respectively. A coupled membrane filter device is installed downstream of the supernatant storage tank. The coupled membrane filter device is connected to the liquid phase outlet of the third centrifuge device and is used to filter the supernatant after centrifugation in the third centrifuge device. The coupled membrane filter device is connected to the pure water supply system. An intermediate storage tank is located downstream of the decolorizing mixing tank, with its inlet connected to the outlet of the decolorizing mixing tank; the acid / alkali outlet of the intermediate storage tank is connected to an acid / alkali supply device. A chromatography system is installed downstream of the intermediate storage tank and is connected to the outlet of the intermediate storage tank. A transfer tank is located downstream of the chromatography system and is connected to the outlet of the chromatography system; the acid and alkali outlet of the transfer tank is connected to an acid and alkali supply device. A sterilization device is installed downstream of the transfer tank, and the sterilization device is connected to the discharge port of the transfer tank for sterilizing the material. A sterile storage tank is located downstream of the sterilization device. The sterile storage tank is connected to the discharge port of the sterilization device, and the acid and alkali outlet of the sterile storage tank is connected to an acid and alkali supply device. A dual-effect concentration system is installed downstream of the sterile storage tank. The inlet of the dual-effect concentration system is connected to the outlet of the sterile storage tank and the coupling membrane filter device, and is used to concentrate the material. A concentrated liquid stirring tank is located downstream of the double-effect concentration system. The concentrated liquid stirring tank is connected to the discharge port of the double-effect concentration system, and the acid and alkali liquid outlet of the concentrated liquid stirring tank is connected to the acid and alkali liquid supply device. A spray dryer is installed downstream of the concentrated liquid mixing tank. The spray dryer is connected to the discharge port of the concentrated liquid mixing tank and is used to spray dry the concentrated liquid to produce protein powder. The ultrafine pulverizer, located downstream of the powder dryer, is used to pulverize and grind protein powder. The hopper located downstream of the ultrafine pulverizer is used to collect the pulverized and ground protein material.
2. The plant protein extraction system as described in claim 1, characterized in that: The grinding device includes a primary colloid mill and a secondary colloid mill connected in series. The feed inlet of the primary colloid mill is equipped with a feed mixing tank, which is connected to the outlet of the soaking tank via an elevator. The outlet of the secondary colloid mill is connected to the receiving and adjusting cylinder.
3. The plant protein extraction system as described in claim 1, characterized in that: The first centrifugation device includes a horizontal centrifuge located upstream and a disc centrifuge located downstream. The feed inlet of the horizontal centrifuge is connected to the discharge outlet of the primary enzymatic hydrolysis tank, and the feed inlet of the disc centrifuge is connected to the liquid phase outlet of the horizontal centrifuge. The liquid phase outlet of the disc centrifuge is connected to the feed inlet of the downstream secondary enzymatic hydrolysis tank. The solid phase outlets of the horizontal centrifuge and the disc centrifuge are refluxed and connected to the feed inlet of the primary enzymatic hydrolysis tank.
4. The plant protein extraction system as described in claim 2, characterized in that: The outlet of the collection tank is connected to the inlet of the feeding mixing tank.
5. The plant protein extraction system as described in claim 4, characterized in that: The outlet of the collection tank is connected to the inlet of the primary enzymatic hydrolysis tank.
6. The plant protein extraction system as described in claim 1, characterized in that: The coupled membrane filtration device includes an upstream ceramic membrane filtration device and a downstream nanofiltration membrane filtration device. The ceramic membrane filtration device has a molecular weight cutoff of 50-100 kDa, and the nanofiltration membrane filtration device has a molecular weight cutoff of 500-530 kDa.
7. The plant protein extraction system as described in claim 1, characterized in that: The liquid phase outlet of the second centrifuge is also connected to the stationary tank.
Citation Information
Patent Citations
Method for extracting rice protein by using rice
CN101695334B