Strain with phosphorus solubilizing and salt-tolerant functions and application thereof
Patent Information
- Application Number
- CN202610781043.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-06-02
- Publication Date
- 2026-08-18
AI Technical Summary
[0006]本发明为了解决现有菌株活性低、功能单一、处理效果差的技术问题，而提供了一株具有解磷、耐盐碱功能的菌株及其应用
[0006] In order to solve the technical problems of low activity, single function and poor treatment effect of existing strains, this invention provides a strain with phosphorus solubilization and salt and alkali tolerance and its application.
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Abstract
Description
Technical Field
[0001] This invention belongs to the field of industrial wastewater biological treatment technology, specifically involving a strain with phosphorus solubilization and salt and alkali tolerance functions. Background Technology
[0002] Industrial wastewater from industries such as chemical, printing and dyeing, and coal chemical industries is generally characterized by high salt content, alkaline pH, and excessive total phosphorus. Direct discharge can easily cause water bodies to become saline and eutrophic, damaging aquatic ecosystems. Deep dephosphorization and water quality improvement of such wastewater have become key challenges in industrial wastewater treatment.
[0003] Currently, the main methods for treating saline-alkali industrial wastewater are physicochemical and biological methods. Physicochemical methods, such as chemical precipitation, membrane separation, and acid-base neutralization, have high treatment efficiency, but they also suffer from drawbacks such as high operating costs, large reagent dosages, easy generation of chemical sludge for secondary pollution, and complex operation, and are difficult to achieve resource utilization. Biological methods, relying on the metabolic activity of microorganisms, have the advantages of low cost, environmental friendliness, and no secondary pollution, and are the mainstream treatment technology. The screening of functional microorganisms is the core key to this technology.
[0004] Existing phosphorus-solubilizing microorganisms used in wastewater treatment are mostly strains of genera such as Bacillus and Pseudomonas. These strains generally suffer from limited functionality and weak salt and alkali tolerance. Most strains can only function in neutral to low-salt environments, and their activity decreases significantly or even becomes inactive in saline-alkali effluent with a salt concentration ≥300 mmol / L and pH ≥9.0, rendering them ineffective for efficient phosphorus removal. Furthermore, most existing strains only possess single phosphorus-solubilizing or salt and alkali-tolerant functions, lacking synergistic functions such as nitrogen fixation and iron carrier production. The scarcity of iron and nitrogen in the effluent further limits the metabolic activity of these strains, resulting in poor treatment performance and low system stability.
[0005] Kosakonia oryzendophytica strains are facultative anaerobic Gram-negative bacteria. Some strains possess plant growth-promoting functions such as phosphorus solubilization and nitrogen fixation. Currently, they are only used in the field of agricultural soil improvement. Existing isolated Kosakonia oryzendophytica strains have limited salt and alkali tolerance and cannot adapt to the harsh environment of industrial wastewater, nor do they possess industrial-grade efficient phosphorus solubilization and comprehensive metabolic functions. Summary of the Invention
[0006] In order to solve the technical problems of low activity, single function and poor treatment effect of existing strains, this invention provides a strain with phosphorus solubilization and salt and alkali tolerance and its application.
[0007] The strain with phosphorus solubilization and salt and alkali tolerance functions of this invention is Kosakonia oryzendophytica MH-14, which is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 37671 and deposit date of February 3, 2026.
[0008] Furthermore, the salt- and alkali-tolerant functionally enhanced fermentation medium for Kosakonia oryzendophytica MH-14 contained the following concentrations: glucose 18.0 g / L, yeast extract 6.0 g / L, ammonium sulfate 4.0 g / L, sodium chloride 29.25 g / L, dipotassium hydrogen phosphate 3.0 g / L, potassium dihydrogen phosphate 1.0 g / L, magnesium sulfate 0.8 g / L, ferrous sulfate 0.005 g / L, humic acid 2.5 g / L, and trisodium citrate 1.5 g / L.
[0009] The microbial agent of the present invention, which has phosphorus solubilization and salt and alkali resistance functions, contains Kosakonia oryzendophytica MH-14.
[0010] Furthermore, the microbial agent can be a liquid or solid form.
[0011] The application of the strain with phosphorus solubility and salt and alkali tolerance described in this invention in the treatment of industrial wastewater.
[0012] This invention screened Kosakonia oryzendophytica MH-14 strain from industrial wastewater sediment. This strain possesses multiple functions, including efficient phosphorus solubilization, strong salt and alkali tolerance, iron carrier production, and nitrogen fixation. It can stably survive and exert metabolic activity in saline-alkali wastewater with a salt concentration ≤500 mmol / L and pH ≤9.8 without the need for additional nitrogen or iron sources to maintain its activity. This solves the technical problems of low activity, single function, and poor treatment effect of existing strains. The Kosakonia oryzendophytica strain of this invention, which combines phosphorus solubilization, salt and alkali tolerance, iron carrier production, and nitrogen fixation, provides a novel microbial resource and technical solution for the deep biological treatment of saline-alkali industrial wastewater, and has good prospects for industrial application.
[0013] Kosakonia oryzendophytica MH-14 is a bacterium belonging to the genus Kosakonia. It is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 37671, deposited on February 3, 2026, at the Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. Attached Figure Description
[0014] Figure 1 The results of siderophore screening for strain MH-14;
[0015] Figure 2 The results of phosphorus solubilization of strain MH-14 on Monkina inorganic phosphorus solid medium;
[0016] Figure 3 The results of nitrogen fixation of strain MH-14 on Assumption nitrogen-fixing solid medium;
[0017] Figure 4 Phylogenetic tree constructed for strain MH-14. Detailed Implementation
[0018] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.
[0019] It should be noted that, unless otherwise specified, the embodiments and features described in the present invention can be combined with each other.
[0020] Example 1: Isolation and screening of the strain (Kosakonia oryzendophytica MH-14) described in this invention.
[0021] 1. Materials and Methods
[0022] 1.1 Experimental Materials
[0023] In September 2025, sediment samples from well A4WSG795, sourced from Shanxi Huangcheng Xiangfu Pharmaceutical Co., Ltd., were transported back to the School of Environmental Science and Engineering at Harbin Institute of Technology via cold chain. 5g of sediment was placed in an Erlenmeyer flask containing glass beads and 50 mL of sterile water in a clean bench and shaken at 180 r / min at room temperature for 30 min. Then, a serial dilution was performed, with 10g of sediment diluted to 10 ... -3 10-4 10 -5 100 μL of each gradient was spread onto LB agar plates, with each gradient repeated three times (different dilutions were used in this example to better screen for selectable single colonies), and incubated at 28°C for 48 h. After 48 h of incubation, strains with different characteristics were selected for isolation, numbered, and cultured separately.
[0024] 1.2 Identification of the siderophore produced by the strain
[0025] The strain obtained by reactivating the isolated and purified strain was transferred to LB agar plates and cultured for 24 h. Then, a single colony was picked with a sterile toothpick and inoculated into Chromeazurol S (CAS) solid detection medium. The culture was inverted at 37 ℃ for 2-3 days. The size of the discoloration zone around the colony was observed. The colony diameter d and the discoloration zone diameter D were measured using the cross-cross method, and D / d was calculated.
[0026] Further testing was conducted on strains exhibiting a distinct color change zone:
[0027] (1) The activated bacterial growth was inoculated into SA iron-limited liquid medium and cultured in a shaker at 37 ˚C for 48 h;
[0028] (2) Transfer the bacterial suspension to be tested after 48 hours of growth to a sterilized 10 mL centrifuge tube and centrifuge at 13,000 rpm for 15 min.
[0029] (3) Transfer the supernatant to a test tube treated with concentrated hydrochloric acid, add a certain amount of freshly prepared CAS detection solution to make the volume ratio of supernatant to CAS detection solution 1:1, mix thoroughly and let stand at room temperature for 1 h.
[0030] (4) Measure the absorbance value (As) at a wavelength of 630 nm. Use double-distilled water as a control to zero the sample. Use the absorbance value (Ar) at a wavelength of 630 nm of the uninoculated SA iron-limiting liquid culture medium mixed with the CAS detection solution as a reference value. Express the iron carrier activity unit as follows:
[0031] Su≈(Ar-As) / Ar×100;
[0032] In the formula: Su is the iron carrier content; Ar is the OD value of the uninoculated SA iron-limited medium and the CAS detection solution; As is the OD value of the SA iron-limited medium supernatant and the CAS detection solution of the inoculated strain.
[0033] When the number of ferrocarrier activity units is less than 10, it is generally considered negative, and the mixture of ferrocarrier and test solution does not show any color change.
[0034] 1.3 Screening of strains with inorganic phosphorus solubility
[0035] The isolated and purified bacterial strains were inoculated onto plates of Monkina inorganic phosphate-solubilizing bacteria medium using sterile toothpicks. Each gradient was repeated three times, and the plates were incubated at 28°C for 24–48 h. Colonies exhibiting inorganic phosphate-solubilizing zones were selected, and the colony diameter d and the diameter D of the inorganic phosphate-solubilizing zone were measured using the cross-crossing method. The D / d ratio was calculated, and strains with high ratios were selected for purification, and their inorganic phosphate-solubilizing effects were repeatedly verified.
[0036] 1.4 Screening of nitrogen-fixing strains
[0037] The isolated and purified strains were inoculated onto Assyrian nitrogen-fixing bacteria agar plates using sterile toothpicks. Each gradient was repeated three times and incubated at 28°C for 24–48 h. Colonies exhibiting a clear zone were selected, and the colony diameter d and the diameter D of the nitrogen-fixing zone (the nitrogen-fixing zone is the diameter of the clear zone) were measured using the cross-crossing method. The D / d ratio was calculated, and strains with high ratios were selected for purification and their nitrogen-fixing effect was repeatedly verified.
[0038] 1.5 Identification of salt-tolerant strains
[0039] LB agar plates containing NaCl at concentrations of 50 mmol / L, 100 mmol / L, 200 mmol / L, 300 mmol / L, 400 mmol / L, 450 mmol / L, 500 mmol / L, 550 mmol / L, 600 mmol / L, and 650 mmol / L were prepared. The isolated and purified bacterial strains were inoculated onto LB agar plates using the streak plating method, with a medium without NaCl serving as a control. The plates were sealed and inverted in a constant temperature incubator at 28 ℃ for 24–48 h, during which colony growth was observed.
[0040] 1.6 Screening of alkali-resistant strains
[0041] LB solid culture media with pH values ranging from 7 to 12.5 were prepared. The isolated and purified bacterial strains were inoculated onto LB solid culture media using the streak plating method, with the pH 7 medium used as a control. The media were sealed and inverted in a constant temperature incubator at 28 °C for 24–48 h, during which colony growth was observed. Further identification was performed based on the colony growth.
[0042] 2 Results and Analysis
[0043] 2.1 Initial screening of strains with siderophore-producing ability
[0044] A total of 187 strains with different morphologies were screened from the sediment. Among them, 12 strains simultaneously exhibited siderophore production, inorganic phosphorus solubilization, and nitrogen fixation. Strain MH-14, with relatively good comprehensive abilities, was selected. The isolated and purified MH-14 strain was inoculated onto CAS medium. After a period of cultivation, a distinct discoloration zone formed around the colony. Figure 1 As shown ( Figure 1 (Screening results for siderophores of strain MH-14) The diameter of the discoloration zone D of strain MH-14 is 17.35 mm, the colony diameter d is 5.03 mm, and the D / d ratio is 3.45, indicating that strain MH-14 has a strong siderophore capable of producing high iron chelation.
[0045] 2.2 Determination of iron-producing capacity
[0046] Determination of siderogenic capacity: The Su value of siderogenic capacity of strain MH-14 at 37℃ was 71.01% using method 1.2, indicating that the strain has a strong siderogenic capacity.
[0047] 2.3 Screening of strains with inorganic phosphorus solubility
[0048] When MH-14 strain was inoculated onto Monkina inorganic phosphorus medium, after a period of cultivation, a distinct zone of dissolved inorganic phosphorus formed around the colony, such as... Figure 2 As shown ( Figure 2 (The results of phosphorus solubilization of strain MH-14 on Monkina inorganic phosphorus solid medium) The diameter of the inorganic phosphorus solubilization zone D of strain MH-14 was 9.10 mm, the colony diameter d was 7.28 mm, and the D / d ratio was 1.25, indicating that strain MH-14 has a certain function of solubilizing inorganic phosphorus.
[0049] 2.4 Screening of nitrogen-fixing strains
[0050] When strain MH-14 was inoculated onto Assumption nitrogen-fixing medium, after a period of cultivation, a distinct nitrogen-fixing zone formed around the colony, such as... Figure 3 As shown ( Figure 3 (The results show the nitrogen fixation of strain MH-14 on Assumption nitrogen-fixing solid medium). The diameter of the nitrogen-fixing zone D of strain MH-14 was 6.02 mm, the colony diameter d was 4.07 mm, and the D / d ratio was 1.48, indicating that strain MH-14 has a certain nitrogen-fixing function.
[0051] 2.5 Identification of Salt Stress Resistance
[0052] Table 1 shows the salt stress resistance of strain MH-14. As can be seen from Table 1, strain MH-14 can grow normally at NaCl concentrations ranging from 0 to 500 mmol / L, indicating that strain MH-14 has strong salt tolerance.
[0053] Table 1. Identification of salt stress resistance in strain MH-14
[0054]
[0055] Note: + indicates growth; - indicates no growth.
[0056] 2.6 Identification of resistance to alkali stress
[0057] Table 2 shows the alkali stress resistance of strain MH-14. As can be seen from Table 2, strain MH-14 can grow normally under pH 9.8 conditions, indicating that strain MH-14 has strong alkali resistance.
[0058] Table 2. Identification of alkaline stress resistance in strain MH-14
[0059]
[0060] Note: + indicates growth; - indicates no growth.
[0061] 2.7 Identification of MH-14 strain
[0062] 2.7.1 Physiological and biochemical identification: The MH-14 strain was streaked in three zones on a solid LB medium plate, and single colonies were isolated and their morphology was described. The strain was Gram-stained and physiologically and biochemically identified according to the "Handbook of Common Bacterial System Identification".
[0063] Physiological and biochemical identification results:
[0064] The colony characteristics of strain MH-14 on LB solid medium were as follows: the colonies were pale yellow, with peritrichous motility, no spores, slightly raised in the center, and moist and viscous on the surface. Gram staining confirmed it to be a Gram-negative bacterium. Table 3 shows the physiological and biochemical results of strain MH-14. Based on the descriptions of the physiological and biochemical characteristics of Kosakonia oryzendophytica in Bergey's Manual of Bacteriology and related literature, MH-14 shares the same physiological and biochemical characteristics as the type species of Kosakonia oryzendophytica. Therefore, based on these physiological and biochemical results, strain MH-14 is likely Kosakonia oryzendophytica.
[0065] Table 3. Physiological and biochemical results of strain MH-14
[0066]
[0067] Note: + indicates a positive result; - indicates a negative result.
[0068] 2.7.2 Identification of 16S rRNA
[0069] DNA was extracted from strain MH-14 using a bacterial genomic DNA extraction kit from Beijing Solarbio Biotechnology Co., Ltd. PCR amplification was performed using universal bacterial primers 27F / 1492R. The PCR amplification system was 25 µL: 2.5 µL 10× buffer, 0.5 µL Taq enzyme, 0.5 µL primer 27F, 0.5 µL primer 1492R, 1 µL DNA template, and 20 µL ddH2O. The reaction program was: 95℃ pre-denaturation for 5 min; 94℃ denaturation for 50 s, 56℃ annealing for 30 s, 72℃ extension for 1.5 min, for 30 cycles; a final extension at 72℃ for 10 min; and storage at 4℃. The PCR products were sent to RuiBiotech for sequencing. The sequencing results of the strain's 16S rRNA were compared with the NCBI database, and a phylogenetic tree was constructed.
[0070] 16S rRNA identification results:
[0071] After sequencing the 16S rRNA sequence, BLAST comparison in NCBI revealed that the 16S rRNA gene sequence of strain MH-14 had a 99% similarity to Kosakonia oryzendophytica. Figure 4 The phylogenetic tree constructed for strain MH-14 shows that strain MH-14 and Kosakonia oryzendophytica (REICA_082) are in the same smallest branch and are closely related in evolution. Based on comprehensive physiological and biochemical indicators, strain MH-14 was identified as Kosakonia oryzendophytica.
[0072] Example 2
[0073] Salt- and alkali-tolerant functionalized fermentation medium adapted to Kosakonia oryzendophytica MH-14 strain:
[0074] Formula (1L system, pH 9.0-9.5):
[0075] Glucose 18.0 g / L, yeast extract 6.0 g / L, ammonium sulfate 4.0 g / L, sodium chloride 29.25 g / L, dipotassium hydrogen phosphate 3.0 g / L, potassium dihydrogen phosphate 1.0 g / L, magnesium sulfate 0.8 g / L, ferrous sulfate 0.005 g / L, humic acid 2.5 g / L, trisodium citrate 1.5 g / L, deionized water 1000 mL.
[0076] Key process parameters:
[0077] pH adjustment: Adjust to 9.0-9.5 using 1mol / L NaOH solution. After sterilization, the pH stabilizes at 8.8-9.3, requiring no further adjustment.
[0078] Sterilization conditions: autoclave at 121℃ for 20 minutes, cool to below 30℃ for aseptic inoculation (avoid high temperature to prevent damage to the activity of nutrient components).
[0079] Inoculation rate: Inoculate Kosakonia oryzendophytica MH-14 seed culture (OD) at 2% (v / v). 600 =0.8-1.0), shortening the strain's adaptation period;
[0080] Fermentation control: temperature 37℃, shaking speed 200rpm (to ensure sufficient dissolved oxygen and adapt to the facultative anaerobic characteristics of the strain), fermentation cycle 24h;
[0081] Endpoint indicator: viable bacteria count ≥ 1.0 × 10⁻⁶ 9 CFU / mL, inorganic phospholytic enzyme activity ≥80U / mL, siderophore SU value ≥70%, and survival rate of the strain ≥90% in an environment of 500mmol / L NaCl and pH9.8.
[0082] Table 4 shows a comparison of the core parameters of the ordinary LB medium and the salt- and alkali-tolerant functionally enhanced fermentation medium of the present invention.
[0083] Table 4 Comparison of core parameters between ordinary LB medium and the salt- and alkali-tolerant functionally enhanced fermentation medium of this invention
[0084]
[0085] Example 3
[0086] This embodiment is applicable to open-type water treatment systems such as industrial wastewater biological treatment ponds and advanced treatment units. Liquid bacterial agents are directly added, allowing the strains to rapidly colonize and perform metabolic functions. The specific steps are as follows:
[0087] Seed culture preparation: The refrigerated Kosakonia oryzendophytica MH-14 strain was inoculated into a salt- and alkali-tolerant functional enhanced fermentation medium and cultured at 37°C and 200 rpm for 24 hours until the strain reached OD. 600 When the value reaches 0.8-1.0, seed solution is obtained;
[0088] Preparation of liquid inoculum: Inoculate the seed culture at a volume ratio of 1%-5% into a salt- and alkali-tolerant functional enhanced fermentation medium, and culture at 37℃ and 200rpm for 24 hours until the viable count of Kosakonia oryzendophytica MH-14 in the fermentation broth is ≥1.0×10⁻⁶.9 CFU / mL yields a liquid bacterial agent that can be added directly.
[0089] Wastewater treatment dosing: Add the above liquid bacterial agent to the treatment unit (biological tank / deep treatment tank) of saline-alkali industrial wastewater at a ratio of 0.5%-3% of the industrial wastewater volume. Control the operating parameters of the treatment unit: temperature 20-37℃, hydraulic retention time (HRT) 24-72h, dissolved oxygen (DO) 0.5-3.0mg / L. The bacterial strains will naturally colonize in the wastewater and perform metabolic functions, efficiently degrading inorganic phosphorus, while maintaining their own activity through the synergistic effect of nitrogen fixation and iron production.
[0090] Subsequent maintenance: Add liquid bacterial agent every 7-10 days at 0.1%-0.5% of the effluent volume to ensure that the viable count of Kosakonia oryzendophytica MH-14 strain in the treatment unit is ≥1.0×10⁻⁶. 6 CFU / mL enables continuous deep treatment of saline-alkali industrial wastewater.
[0091] The comparison results of water quality indicators before and after treatment of saline-alkali industrial wastewater using the method of this embodiment are shown in Table 5 (n is the number of test samples, n=5, the data in the table are the average values of the measurements, treatment time: hydraulic retention time 48h).
[0092] Table 5 Comparison of water quality indicators before and after treatment of saline-alkali industrial effluent with liquid bacterial agent
[0093]
[0094] Example 4
[0095] This embodiment is applicable to industrial wastewater continuous flow treatment systems. Immobilized bacterial agents can improve the stress resistance and reusability of bacterial strains, reduce strain loss, and enhance the stability of the treatment system. The steps are as follows:
[0096] Preparation of seed culture and fermentation broth: The refrigerated Kosakonia oryzendophytica MH-14 strain was inoculated into a salt- and alkali-tolerant functional enhanced fermentation medium and cultured at 37°C and 200 rpm for 24 hours until the strain reached OD. 600 When the value reaches 0.8-1.0, seed solution is obtained;
[0097] Fermentation broth preparation: The seed culture was inoculated into LB liquid fermentation medium at a volume ratio of 1%-5%, and cultured at 37℃ and 200rpm for 24 hours to obtain Kosakonia oryzendophytica MH-14 with a viable count ≥1.0×10⁻⁶. 9 Fermentation broth with CFU / mL;
[0098] Preparation of immobilized bacterial agent: Sodium alginate-calcium chloride was selected as the immobilization carrier. Sodium alginate was dissolved in sterile water to prepare a 2%-4% (w / w) sodium alginate solution. The sodium alginate solution was mixed with the fermentation broth at a volume ratio of 1:1. After thorough mixing, the mixture was dripped into a 2%-3% (w / w) calcium chloride solution using a syringe to form gel microspheres with a diameter of 3-5 mm. The microspheres were cross-linked and cured at room temperature for 2-4 hours. The gel microspheres were collected by filtration and washed 2-3 times with sterile physiological saline to obtain the immobilized bacterial agent loaded with the MH-14 strain, wherein the viable count of Kosakonia oryzendophytica MH-14 strain was ≥5.0 × 10⁻⁶. 8 CFU / g;
[0099] Immobilized bacterial agent loading and wastewater treatment: Immobilized bacterial agent is loaded into an immobilized reaction column for deep treatment of industrial wastewater, with a loading volume of 60%-80% of the effective volume of the reaction column. Saline-alkali industrial wastewater is pumped into the reaction column and treated in a continuous flow mode. The operating parameters are controlled as follows: temperature 20-37℃, hydraulic retention time (HRT) 12-48h, influent salt concentration ≤500mmol / L, and influent pH 7.0-9.8. When the wastewater passes through the immobilized bacterial agent layer, the bacterial strains degrade inorganic phosphorus in the wastewater through phosphorus solubilization metabolism. The nitrogen fixation and iron carrier functions work together to maintain the activity of the bacterial strains.
[0100] Microbial agent maintenance and regeneration: Immobilized microbial agents can be used continuously for 30-45 days. If the treatment effect decreases, the microbial agents in the reaction column can be rinsed with sterile physiological saline and then soaked in fresh MH-14 strain fermentation broth for 24 hours to regenerate the microbial agents. After regeneration, the microbial agents can be used again.
[0101] Table 6 shows the comparison of water quality indicators before and after treatment of saline-alkali industrial wastewater using the method of this embodiment (n is the number of test samples, n=5, the data in the table are the average values of the measurements, treatment time: continuous operation for 30 days, single retention for 24 hours).
[0102] Table 6 Comparison of water quality indicators before and after treatment of saline-alkali industrial effluent with immobilized bacterial agents.
[0103]
Claims
1. A strain of bacteria possessing phosphorus solubilization and salt-alkali tolerance functions, characterized in that, The strain with phosphorus solubilization and salt and alkali tolerance is Kosakonia oryzendophytica MH-14, which is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 37671 and deposit date of February 3, 2026.
2. The strain with phosphorus solubilization and salt-alkali tolerance functions according to claim 1, characterized in that, The salt- and alkali-tolerant functional enhanced fermentation medium for Kosakonia oryzendophytica MH-14 contained the following concentrations: glucose 18.0 g / L, yeast extract 6.0 g / L, ammonium sulfate 4.0 g / L, sodium chloride 29.25 g / L, dipotassium hydrogen phosphate 3.0 g / L, potassium dihydrogen phosphate 1.0 g / L, magnesium sulfate 0.8 g / L, ferrous sulfate 0.005 g / L, humic acid 2.5 g / L, and trisodium citrate 1.5 g / L.
3. A microbial agent with phosphorus-solubilizing and salt-alkali-tolerant functions, characterized in that, Microbial agents with phosphorus solubilizing and salt-alkali tolerant functions include Kosakonia oryzendophytica MH-14 as described in claim 1.
4. The microbial preparation according to claim 3, characterized in that, Microbial agents can be in liquid or solid form.
5. The application of the strain with phosphorus solubility and salt and alkali tolerance as described in claim 1 in the treatment of industrial wastewater.