A multi-band light source switchable discontinuous chemical analyzer and adaptive compensation method

Abstract

This invention discloses a multi-band switchable intermittent chemical analyzer and its adaptive compensation method, belonging to the technical field of intermittent chemical analyzers. The analyzer includes a chassis and within it, reagent storage areas, sample storage areas, pipette tip storage areas, cuvette holders, pipettes, and optical detection components. The cuvette holder has multiple cuvette placement slots, with light-transmitting holes on opposite sidewalls. The pipettes are driven by a three-axis drive assembly to achieve liquid transfer and tip placement / removal. The optical detection component includes a rotating disk-type wavelength switching mechanism and a shared photoelectric receiving module. Multiple light sources of different wavelengths can be selectively introduced into the detection optical path and aligned with the light-transmitting holes and the shared photoelectric receiving module. This invention enables automatic multi-band switching and shared receiving detection, reducing channel differences and improving detection consistency and automation.

Description

Technical Field

[0001] This invention belongs to the technical field of intermittent chemical analyzers, and specifically relates to an intermittent chemical analyzer with switchable multi-band light sources and an adaptive compensation method. Background Technology

[0002] Intermittent chemical analyzers are a detection method that uses the absorption characteristics of a sample to light of a specific wavelength to calculate the sample concentration. They are widely used in water quality analysis, biochemical analysis, immunoassay, and environmental monitoring. Existing automated intermittent chemical analyzers typically include a reagent storage area, a sample storage area, a reaction detection area, a pipetting mechanism, and an optical detection mechanism. The sample and reagents are added to a cuvette via the pipetting mechanism, and the optical detection mechanism collects the transmitted light signal and calculates the sample concentration based on the absorbance and a standard curve.

[0003] With the increasing number of testing items and the growing demand for low-concentration detection, existing intermittent chemical analyzers still have shortcomings in practical use. Firstly, multi-wavelength detection usually requires multiple light sources or multiple optical channels. Differences in installation position, luminous intensity, receiving sensitivity, and aging degree between different light sources, receivers, or detection channels can lead to a decrease in the consistency of detection results between different bands or different slots.

[0004] Secondly, batch testing requires multiple cuvettes to participate in the reaction and measurement. However, cuvettes may vary in wall thickness, transmittance, refractive properties, and surface condition during manufacturing. They may also develop dust, droplet residue, or minor scratches during use. These differences can alter the transmitted light signal. Especially when detecting low-concentration samples, minute background deviations may be amplified during concentration calculations, affecting the accuracy of the test results. Summary of the Invention

[0005] The purpose of this invention is to provide an intermittent chemical analyzer and an adaptive compensation method that can share the photoelectric receiving foundation in multi-band detection, reduce multi-channel differences, and dynamically predict and compensate the liquid background of each test cell without injecting reference liquid into each test cell individually.

[0006] The present invention is achieved through the following measures: a multi-band light source switchable intermittent chemical analyzer, characterized in that it includes a chassis, wherein the chassis is provided with a reagent storage area, a sample storage area, a pipette tip storage area, a cuvette holder, a pipette, and an optical detection component; The cuvette holder is provided with a plurality of cuvette slots for placing cuvettes, and each cuvette slot has a light-transmitting hole on its opposite side wall that is in the detection optical path. The pipette is driven by a three-axis drive assembly and is used to transfer liquids and pick up / place pipette tips between the reagent storage area, the sample storage area, the pipette tip storage area and the cuvette holder. The optical detection assembly includes a wavelength switching mechanism and a shared photoelectric receiving module. The wavelength switching mechanism includes a rotating disk and a servo motor that drives the rotating disk to rotate. The rotating disk is circumferentially spaced with multiple light sources of different wavelengths. The rotating disk and the shared photoelectric receiving module are located on opposite sides of the cuvette holder. By rotating the rotating disk, any light source can enter the detection position opposite to the shared photoelectric receiving module and form the same detection optical path with the shared photoelectric receiving module and the light-transmitting hole of the corresponding cuvette station slot. The shared photoelectric receiving module is used to receive the detection light signal transmitted through the cuvette.

[0007] Furthermore, the plurality of cuvette station slots are arranged at intervals along the length of the cuvette support; the optical detection assembly further includes a driving mechanism, which drives the wavelength switching mechanism and the shared photoelectric receiving module to reciprocate synchronously along the length of the cuvette support, so that the detection optical path is sequentially aligned with the light-transmitting holes of each cuvette station slot. The cuvette station slots are disposed on the cuvette support.

[0008] Furthermore, the bottoms of the shared photoelectric receiving module and the servo motor are both fixed on the same mounting base.

[0009] Furthermore, it also includes an ultrasonic cleaning mechanism for cleaning the cuvette; The ultrasonic cleaning mechanism includes a transducer and an ultrasonic needle located below the transducer. The ultrasonic needle has a concentrically arranged channel with an open bottom. The upper part of the ultrasonic needle is provided with a flexible tube connected to the channel. The other end of the flexible tube is connected to the first connection port of a tee. The second connection port of the tee is connected to a cleaning water pump through a water inlet pipe. The cleaning water pump is connected to a cleaning water tank. The third connection port of the tee is connected to a drain pipe. Valves are provided on both the water inlet pipe and the drain pipe. It also includes a second drive mechanism that enables the ultrasonic cleaning mechanism to reciprocate linearly along the Z-axis, and the second drive mechanism is fixed on the output end of the first drive mechanism.

[0010] Furthermore, a horizontal partition is provided inside the chassis, and the reagent storage area includes a sinkhole provided on the horizontal partition and a reagent tube rack provided in the sinkhole; The bottom surface of the sinking trough is an inclined surface that slopes towards the drain outlet, and a water collection trough is provided on the bottom plate of the chassis and below one side of the drain outlet.

[0011] Furthermore, the reagent tube rack includes a base plate and a top plate, which are fixedly connected by several support plates. The top plate has reagent tube holes for inserting the reagent tubes, and the base plate has reagent tube positioning holes corresponding to the reagent tube holes. The bottom of the reagent tube is inserted into the positioning hole.

[0012] Furthermore, the sample storage area is a plurality of sample placement slots disposed on the horizontal partition; The sample is placed in the placement slot via a tray. At least one tray positioning hole is provided on the partition plate outside the placement slot. The tray is provided with tray positioning posts that mate with the tray positioning hole. The tray is provided with several test tube insertion holes.

[0013] Furthermore, the pipette tip storage area is a mounting slot provided on the horizontal partition. A pipette tip storage box is placed in the mounting slot, and a mesh plate with several insertion holes is snapped onto the top of the storage box. The pipette tips are inserted into the insertion holes. A pipette tip recycling box is provided on the horizontal partition and on one side of the mounting slot.

[0014] Furthermore, the cuvette holder is fixed to the bottom plate of the chassis, and the horizontal partition is provided with a strip-shaped opening that allows the cuvette holder to be exposed.

[0015] Furthermore, it also includes a refrigeration mechanism, which includes a cooler, a cooling fan, a hot water exchange tank, a cooling water pump, a circulating water tank, and a heat exchanger; the cooling fan is located on the side of the cooler facing the sinking trough, the hot water exchange tank is located on the other side of the cooler, and the hot water exchange tank, the cooling water pump, the circulating water tank, and the heat exchanger are connected in sequence through pipelines; the sinking trough is arranged adjacent to the cuvette holder.

[0016] Furthermore, the three-axis drive assembly includes symmetrically arranged Y-axis slide rails on the upper part of the chassis, Y-axis sliders slidably arranged on both Y-axis slide rails, an X-axis slide rail between the two Y-axis sliders, an X-axis slider slidably arranged on the X-axis slide rail, a Z-axis slide rail fixedly arranged on the X-axis slider, a Z-axis slider slidably arranged on the Z-axis slide rail, and the pipette fixedly arranged on the Z-axis slider; The three-axis drive assembly also includes a drive mechanism three for driving the Y-axis slider, X-axis slider and Z-axis slider.

[0017] Furthermore, both ends of the two Y-axis slide rails are fixed to the bottom plate of the chassis by columns at both ends.

[0018] The drive mechanism one, drive mechanism two, and drive mechanism three can all be one of a lead screw, a linear motor, or a synchronous belt. Each of the drive mechanism one, drive mechanism two, and drive mechanism three is equipped with a protective cover, depending on the circumstances.

[0019] Furthermore, the chassis also includes side panels arranged around the chassis bottom plate and a chassis top plate connecting the four side panels. An L-shaped operating port is opened at the connection between the chassis top plate and the front side plate, and an L-shaped cover plate is provided on the L-shaped operating port. The upper end of the L-shaped cover plate is hinged to the chassis top plate.

[0020] A transparent observation port is provided on the L-shaped cover.

[0021] This embodiment provides an adaptive compensation method based on the intermittent chemical analyzer, characterized in that it includes: S1. Under the light-off state, the dark current background value is collected through the same photoelectric receiving module and recorded as follows: ; S2. Determine the target detection wavelength based on the current detection item, and control the servo multi-wavelength switching mechanism to switch the target detection wavelength to the required light source; S3. Select the cuvette station slot at the starting position as the reference slot. Ensure all cuvettes in the reference slot and each cuvette station slot are in a dry, unfilled state. Drive the optical detection component to sequentially move to the reference slot and each of the cuvette station slots. Collect the empty-cuvette transmission response value of the reference slot using the same photoelectric receiving module. The transmission response values ​​of empty cuvettes in each cuvette station were collected; among them, the first... The transmission response value of the empty cup in each cuvette station is denoted as . , It is a positive integer; S4. After adding reference liquid to the reference tank and completing the corresponding reaction, the liquid blank reference response value under the target light source is acquired through the same photoelectric receiving module. ; S5. Based on the liquid blank reference response value The empty cup transmission response value of the reference groove , No. Transmission response value of an empty cup in a cuvette station. and dark current Predicting the first Ideal liquid background response value of each cuvette station tank when filled with reference liquid Wherein, the ideal liquid background response value Calculate using the following formula:

[0022] S6, To the After the sample to be tested is added to each cuvette station and the corresponding reaction is completed, the transmission response value of the sample is acquired through the same photoelectric receiving module. And based on the ideal liquid background response value The sample transmission response value and the system's dark current background value Calculate the first The absorbance of each cuvette station Wherein, the absorbance Calculate using the following formula:

[0023] In the formula, Indicates the first The absorbance of each cuvette station. Indicates the first The transmission response value of the sample in each cuvette station cell; S7. Based on the absorbance... The concentration of the sample to be tested is obtained by using a standard curve corresponding to the detection wavelength of the target light source.

[0024] In step S3, for the same slot, a preset number of empty cup photoelectric response data are continuously collected, the median of the preset number of empty cup photoelectric response data is calculated, abnormal empty cup photoelectric response data are removed according to the median and a preset empty cup response fluctuation threshold, and the average value of the remaining valid empty cup photoelectric response data is calculated to obtain the empty cup transmission response value corresponding to the slot. In step S1, a preset number of dark current response data are continuously collected, the median of the preset number of dark current response data is calculated, abnormal dark current response data are removed based on the median and a preset dark current fluctuation threshold, and the average value of the remaining valid dark current response data is calculated to obtain the dark current background value. .

[0025] Before step S5, the process also includes the first... Transmission response value of an empty cup in a cuvette station. The empty cup transmission response value of the reference groove Compensation ratio between To determine the effectiveness, the compensation ratio is... Calculate using the following formula:

[0026] In the formula, Indicates the first The compensation ratio of the empty cup of each cuvette station relative to the reference station; when the compensation ratio When the value exceeds the preset allowable range, the first... The cuvettes corresponding to the cuvette station tanks exhibit abnormal light transmission, and the first cuvette... Each cuvette station will prompt for retesting, rejection, or replacement.

[0027] S8. During multi-wavelength detection, steps S2 to S7 are executed for each target detection wavelength. That is, for each target detection wavelength, the corresponding system dark current background value is obtained. , Reference slot empty cup transmission response value , No. Transmission response value of an empty cup in a cuvette station. Liquid blank reference response value Ideal liquid background response value Sample transmission response value and absorbance The concentration of the corresponding detection parameters is calculated based on the standard curve corresponding to the detection wavelength of each target.

[0028] In this embodiment, a controller is also installed inside the chassis. The controller can be a microcontroller, a programmable logic controller, an embedded control board, or an industrial control computer. The controller is electrically connected to the pipette, drive mechanism one, drive mechanism two, drive mechanism three, servo motor, each light source, a shared photoelectric receiving module, a cleaning water pump, a cooling water pump, valves, a transducer, and a cooler. The controller is used to control the pipette to complete tip placement, sample aspiration, reagent aspiration, liquid addition, and waste tip recycling according to a preset detection process; control the servo motor to rotate the rotating disk so that the target light source enters the detection optical path; control drive mechanism one to move the optical detection component to the corresponding cuvette station slot; control the shared photoelectric receiving module to collect dark current response, empty cup transmission response, liquid blank reference response, and sample transmission response; and perform dark current subtraction, empty cup ratio compensation, absorbance calculation, and concentration calculation based on the collected photoelectric response data.

[0029] The beneficial effects of the technical solution provided by the embodiments of the present invention are as follows: This invention arranges multiple light sources on a rotating disk, which is rotated by a servo motor. This allows light sources of different detection wavelengths to be selectively placed on the same detection optical path with a shared photoelectric receiving module. Therefore, different wavelength bands can share the same photoelectric receiving module, reducing response differences between multiple receivers or detection channels and improving the consistency of multi-band detection results.

[0030] This invention places the cuvette holder between the rotating disk and the shared photoelectric receiving module. A light-transmitting hole is provided on the cuvette station slot, positioned within the detection optical path. Transmission detection is completed simply by placing the cuvette within the station slot. During the detection process, the cuvette remains on the cuvette holder, eliminating the need to transfer it to a separate detection position after the reaction. This helps reduce liquid sloshing, bubble adhesion, and repetitive positioning errors.

[0031] This invention uses a driving mechanism to move a wavelength switching mechanism and a shared photoelectric receiving module along the length of the cuvette holder, enabling the same optical detection component to sequentially detect multiple cuvette workstations, thereby achieving automatic scanning and detection of batch samples while ensuring that the cuvettes remain stationary.

[0032] This invention collects the dark current background value before testing, and collects the empty cup transmission response values ​​of the reference tank and each cuvette station tank in a dry cup state. Only the reference tank is measured for liquid blank reference. Based on the liquid blank reference response value of the reference tank, the empty cup transmission response value of the reference tank, and the empty cup transmission response value of the cuvette station tank, the ideal liquid background response value of the cuvette station tank when filled with reference liquid is predicted. This reduces the impact of differences in cuvette transmittance, tank position differences, and changes in light source conditions on absorbance calculation.

[0033] This invention eliminates the need for blank calibration by injecting reference liquid into each cuvette station individually. Instead, it obtains the ideal liquid background for each station by simply scanning with a dry cup and measuring the liquid blank at a single point. This reduces the consumption of reference liquid, deionized water, and reagents, shortens calibration time, and improves batch testing efficiency.

[0034] This invention can also clean cuvettes using an ultrasonic cleaning mechanism, control the temperature of the reagent storage area using a cooling mechanism, and centrally control the pipette, servo motor, drive mechanism, shared photoelectric receiving module, cleaning mechanism, and cooling mechanism via a controller, achieving automated operation of sample addition, reaction, detection, compensation calculation, and cleaning maintenance. Furthermore, the vibration generated by the ultrasonic needle in the ultrasonic cleaning mechanism can mix the sample to be tested in the cuvette before the reaction. Attached Figure Description

[0035] To more clearly illustrate the technical solution of the present invention, the accompanying drawings used in the embodiments will be briefly introduced below. Obviously, the drawings listed below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0036] Figure 1 This is a schematic diagram of the overall structure of an intermittent chemical analyzer with switchable multi-band light sources according to an embodiment of the present invention. Figure 2This is a schematic diagram of the structure of an intermittent chemical analyzer with the L-shaped cover plate removed. Figure 3 This is a schematic diagram of the structure of an intermittent chemical analyzer after removing the L-shaped cover, top plate, side plates, and protective cover. Figure 4 yes Figure 3 A magnified view of part B in the image; Figure 5 This is a schematic diagram of the structure of an intermittent chemical analyzer after removing the L-shaped cover plate, top plate, side plate, protective cover, and refrigeration mechanism cover plate. Figure 6 yes Figure 5 A magnified view of a section at point C; Figure 7 This is a structural diagram showing the removal of the L-shaped cover, top plate, side plates, protective cover, refrigeration mechanism cover, horizontal partition, and related components; Figure 8 This is a structural schematic diagram of the ultrasonic needle and its related components; Figure 9 This is a schematic diagram of the reagent tube rack structure; Figure 10 This is a schematic diagram of the pipette tip storage box; Figure 11 This is a schematic diagram of the tray structure; Figure 12 This is a structural diagram of a horizontal partition; Figure 13 This is a schematic diagram of the optical detection component; Figure 14 This is a structural schematic diagram of the wavelength switching mechanism and its related components; Figure 15 Is with Figure 13 Schematic diagrams of structures at different angles; Figure 16 yes Figure 15 A magnified view of a section at point A in the middle; Figure 17 This is a flowchart of an adaptive compensation method based on an intermittent chemical analyzer.

[0037] The components represented by each number in the attached diagram are listed below: 1. Chassis; 2. Reagent storage area; 3. Sample storage area; 4. Pipette tip storage area; 5. Cuvette holder; 6. Wavelength switching mechanism; 7. Drive mechanism one; 8. Synchronous belt drive mechanism; 9. Limit sensor; 10. Protective cover; 11. Pipette tip recycling box; 12. Light shield; 13. Mounting base; 14. Y-axis slide rail; 15. Y-axis slider; 16. X-axis slide rail; 17. X-axis slider; 18. 103. Pipette; 19. Z-axis slider; 20. Z-axis slide rail; 21. Ultrasonic cleaning mechanism; 22. Synchronous slider; 23. Cooling mechanism; 24. Cleaning water tank; 25. Cleaning water pump; 106. Chassis bottom plate; 107. Chassis top plate; 108. Horizontal partition; 109. Column; 1001. Sample placement slot; 1002. Mounting slot; 1004. Sinking tank; 1005. Inclined surface; 1006. Drain outlet; 1007. Cooling fan mounting port; 1008. Strip 201. Reagent tube rack; 20101. Tube rack top plate; 20102. Tube rack bottom plate; 20103. Tube rack support plate; 20104. Reagent tube hole; 20105. Reagent tube limiting hole; 301. Tray; 302. Limiting post; 401. Pipette tip storage box; 402. Mesh plate; 403. Insertion hole; 404. Flexible clamping plate; 501. Cuvette station slot; 502. Light transmission hole; 601. Rotary disk; 602. Light source; 603. Servo... Serving motor; 604, Shared photoelectric receiving module; 701, Base 1; 702, Slide rail 1; 703, Lead screw 1; 704, Connecting plate; 705, Motor 1; 706, Lead screw nut 1; 901, Sensing element; 902, Detection element; 1801, Pipette tip; 2101, Transducer; 2102, Ultrasonic needle; 2103, Hose interface; 2301, Hot water tank; 2302, Cooling water pump; 2303, Heat exchanger; 2304, Circulating water tank. Detailed Implementation

[0038] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to embodiments. Of course, the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0039] Example 1: This embodiment provides a discontinuous chemical analyzer with switchable multi-band light sources, suitable for automated detection scenarios such as water quality testing, biochemical testing, immunoassay, and other applications requiring calculation of sample absorbance through changes in transmitted light intensity. This discontinuous chemical analyzer can sequentially detect multiple cuvette positions 501 using movable optical detection components while the cuvettes remain stationary. It acquires transmitted light signals from different positions and at different detection wavelengths using the same photoelectric receiving module, thereby reducing optical differences between multiple detection channels.

[0040] See Figures 1-16 A multi-band light source switchable intermittent chemical analyzer, characterized in that it includes a chassis 1, and the chassis 1 is provided with a reagent storage area 2, a sample storage area 3, a pipette tip 1801 storage area 4, a cuvette holder 5, a pipette 18, and an optical detection component. The cuvette holder 5 is provided with multiple cuvette placement slots 501 for placing cuvettes, and each cuvette placement slot 501 has a light-transmitting hole 502 on its opposite side wall that is in the detection optical path; the cuvette placement slots 501 are provided on the cuvette holder.

[0041] The pipette 18 is driven by a three-axis drive assembly and is used to transfer liquids and pick up / place pipette tips 1801 between the reagent storage area 2, sample storage area 3, pipette tip 1801 storage area 4, and cuvette holder 5. The pipette 18 utilizes existing technology and is detachably connected to disposable pipette tips 1801. The controller moves the pipette 18 to the pipette tip 1801 storage area 4 to pick up the pipette tip 1801. After completing the sample or reagent transfer, the controller moves the pipette 18 to the pipette tip 1801 collection box 11, and a detachment mechanism detaches the used pipette tip 1801 into the collection box 11. Using disposable pipette tips 1801 for sample and reagent transfer reduces cross-contamination between different samples or reagents.

[0042] The optical detection assembly includes a wavelength switching mechanism 6 and a shared photoelectric receiving module 604. The wavelength switching mechanism 6 includes a rotating disk 601 and a servo motor 603 that drives the rotating disk 601 to rotate. Multiple light sources 602 of different wavelength bands are circumferentially spaced on the rotating disk 601. The rotating disk 601 and the shared photoelectric receiving module 604 are located on opposite sides of the cuvette holder 5. Through the rotation of the rotating disk 601, any light source 602 can enter the detection position opposite to the shared photoelectric receiving module 604 and form the same detection optical path with the shared photoelectric receiving module 604 and the light-transmitting hole 502 of the corresponding cuvette station slot 501. The shared photoelectric receiving module 604 is used to receive the detection light signal transmitted through the cuvette. The multiple light sources 602 can be multiple LED light sources 602 with different center wavelengths, and each LED light source 602 is circumferentially spaced along the rotating disk 601. A collimating lens can be provided on the light-emitting side of each LED light source 602 to convert the divergent light emitted by the LED into a collimated beam. The rotating disk 601 can also be provided with conductive contacts corresponding to each LED light source 602. The mounting base 13 is provided with elastic contact pins that cooperate with the conductive contacts. When the rotating disk 601 rotates to the position corresponding to the target light source 602 and the detection optical path, the conductive contact corresponding to the target light source 602 contacts and conducts electricity with the elastic contact pin, so that the target light source 602 is lit, while the other light sources 602 remain in the off state.

[0043] In one specific embodiment, the shared photoelectric receiving module 604 may include a photodetector, a filter element, a preamplifier circuit, and an analog-to-digital converter circuit. The photodetector is used to convert the detection light signal transmitted through the cuvette into a photocurrent signal, the preamplifier circuit is used to amplify the photocurrent signal, and the analog-to-digital converter circuit is used to convert the amplified analog signal into digital photoelectric response data and send it to the controller.

[0044] In one specific embodiment, the number of light sources 602 is determined according to the actual situation. The light sources 602 are generally selected with specific center wavelengths in the visible to near-infrared band, such as 420nm (visible light region, used for phenolphthalein alkalinity detection), 480nm (visible light region, used for chloride detection), 510nm (visible light region, used for hardness and iron detection), 520nm (visible light region, used for nickel detection), 550nm (visible light region, used for total alkalinity detection), 630nm (visible light region, used for zinc detection), 660nm (visible light region, used for sulfide detection), 700nm (visible light region, used for ammonia nitrogen and total phosphorus detection), 810nm (near-infrared region, used for silicate detection), and 880nm (near-infrared region, used for phosphate detection). The half-width at half-maximum (FWHM) of each light source 602 is ≤15nm, and the luminous intensity stability is ≥99.9% (continuous operation for 1 hour), ensuring the specificity of the detection band and the consistency of the output of the light source 602.

[0045] Multiple cuvette station slots 501 are arranged at intervals along the length of the cuvette support 5; the optical detection assembly also includes a drive mechanism 7, which drives the wavelength switching mechanism 6 and the shared photoelectric receiving module 604 to move back and forth synchronously along the length of the cuvette support 5, so that the detection optical path is aligned with the light-transmitting hole 502 of each cuvette station slot 501 in sequence.

[0046] The bottoms of the shared photoelectric receiving module 604 and the servo motor 603 are both fixed on the same mounting base 13.

[0047] In one embodiment, an ultrasonic cleaning mechanism 21 for cleaning contrast cuvettes is also included; The ultrasonic cleaning mechanism 21 includes a transducer 2101 and an ultrasonic needle 2102 disposed below the transducer 2101. The ultrasonic needle 2102 has a concentric channel with an open bottom. The upper part of the ultrasonic needle 2102 is provided with a hose interface 2103, which is connected to a hose. The other end of the hose is connected to the first connection port of a tee. The second connection port of the tee is connected to a cleaning water pump 25 through a water inlet pipe. The cleaning water pump 25 is connected to a cleaning water tank 24. The third connection port of the tee is connected to a drain pipe. Valves are provided on both the water inlet pipe and the drain pipe. It also includes a second drive mechanism that enables the ultrasonic cleaning mechanism 21 to reciprocate linearly along the Z-axis. The second drive mechanism is fixed to the output end of the first drive mechanism 7. During cleaning, the controller controls the second drive mechanism to lower the ultrasonic needle 2102 into the cuvette, opens the valve on the water inlet pipe and starts the cleaning water pump 25, so that cleaning water enters the cuvette through the ultrasonic needle 2102; then the transducer 2101 is started, so that the ultrasonic needle 2102 generates ultrasonic vibration and forms a cavitation cleaning effect in the cuvette to remove the sample, reagent or colorimetric product remaining on the inner wall of the cuvette; after cleaning is completed, the valve on the water inlet pipe is closed and the valve on the drain pipe is opened to discharge the cleaning waste liquid in the cuvette through the drain pipe.

[0048] The chassis 1 is provided with a horizontal partition 103, and the reagent storage area 2 includes a sink 10304 provided on the horizontal partition 103 and a reagent tube rack 201 provided in the sink 10304. The bottom surface of the settling tank 10304 is an inclined surface 10305 sloping towards the drain outlet 10306. A water collection tank is provided on the bottom plate 101 of the chassis, located below the drain outlet 10306 on one side. Thus, when there is condensate or cleaning residue in the reagent storage area 2, the liquid can flow along the inclined surface 10305 of the bottom surface of the settling tank 10304 to the drain outlet 10306 and enter the water collection tank, reducing the accumulation of liquid in the reagent storage area 2.

[0049] The reagent tube rack 201 includes a base plate 20102 and a top plate 20101. The base plate 20102 and the top plate 20101 are fixedly connected by several support plates 20103. The top plate 20101 has reagent tube holes 20104 for inserting reagent tubes, and the base plate 20102 has reagent tube limiting holes 20105 corresponding to the reagent tube holes 20104. The bottom of the reagent tube is inserted into the reagent tube limiting hole 20105. In use, after the reagent tube passes through the reagent tube hole 20104, its bottom is inserted into the corresponding reagent tube limiting hole 20105, thus ensuring that the reagent tube remains stable when the pipette 18 draws reagents.

[0050] Specifically, the sample storage area 3 consists of several sample placement slots 10301 set on the horizontal partition 103; The sample is placed in the placement slot via tray 301. At least one tray 301 positioning hole is provided on the partition plate outside the placement slot. Tray 301 has a tray 301 positioning post 302 that mates with the tray 301 positioning hole. Tray 301 has several test tube insertion holes. The cooperation of the tray 301 positioning hole and the tray 301 positioning post 302 improves the positioning stability of the sample tray 301.

[0051] Specifically, the pipette tip 1801 storage area 4 is a mounting groove 10302 set on the horizontal partition 103. The pipette tip storage box 401 is placed in the mounting groove 10302. A grid plate 402 with several insertion holes 403 is snapped on the top of the storage box. The grid plate 402 is snapped onto the pipette tip storage box 401 by an elastic retaining plate 404. The pipette tip 1801 is inserted into the insertion hole 403. A pipette tip 1801 recycling box 11 is set on the horizontal partition 103 and on one side of the mounting groove 10302.

[0052] Specifically, the cuvette holder 5 is fixed on the chassis base plate 101, and the horizontal partition 103 is provided with a strip-shaped opening 10308 that allows the cuvette holder 5 to be exposed, so that the pipette 18 can perform liquid addition, liquid removal or liquid transfer operations on the cuvette on the cuvette holder 5 from above the horizontal partition 103.

[0053] In one feasible embodiment, a refrigeration mechanism 23 is also included, which includes a refrigerator, a refrigeration fan, a hot water tank 2301, a refrigeration water pump 2302, a circulating water tank 2304, and a heat exchanger 2303. The refrigeration fan is located on the side of the refrigerator facing the sink 10304, and a refrigeration fan mounting port 10307 is provided on the side wall of the sink 10304. The hot water tank 2301 is located on the other side of the refrigerator. The hot water tank 2301, the refrigeration water pump 2302, the circulating water tank 2304, and the heat exchanger 2303 are connected sequentially through pipelines. The sink 10304 is arranged adjacent to the cuvette holder 5.

[0054] The cooler is attached to the outer surface of the aluminum shell of the hot water tank 2301, and a cooling fan is provided on the other side. The cooling fan blows low-temperature gas to one side of the sink 10304, and the heat of the cooler is carried away by the circulating medium in the hot water tank 2301 and exchanged through the heat exchanger 2303.

[0055] Specifically, the three-axis drive assembly includes Y-axis slide rails 14 symmetrically arranged on the upper part of the chassis 1, Y-axis sliders 15 slidably arranged on both Y-axis slide rails 14, an X-axis slide rail 16 arranged between the two Y-axis sliders 15, an X-axis slider 17 slidably arranged on the X-axis slide rail 16, a Z-axis slide rail 20 fixedly arranged on the X-axis slider 17, a Z-axis slider 19 slidably arranged on the Z-axis slide rail 20, and a pipette 18 fixedly arranged on the Z-axis slider 19; The three-axis drive assembly also includes a drive mechanism three that drives the movement of the Y-axis slider 15, the X-axis slider 17, and the Z-axis slider 19.

[0056] Specifically, both ends of the two Y-axis slide rails 14 are fixed to the chassis base plate 101 by the two end columns 104.

[0057] In one feasible embodiment, drive mechanism 7, drive mechanism 2, and drive mechanism 3 can all be a lead screw, a linear motor, or a synchronous belt. Drive mechanism 7, drive mechanism 2, and drive mechanism 3 are all provided with protective covers 10, depending on the circumstances.

[0058] A lead screw mechanism generally includes a lead screw, a guide rail, a slider, a servo motor 603, and a position detection element 902. The controller performs closed-loop control of the position of the pipette 18 through the position signal fed back by the position detection element 902. This is existing technology, and the specific structure will not be described in detail here. For example, the drive mechanism 7 is a lead screw mechanism, and the mounting base 13 is fixed to the lead screw nut 706 of the lead screw mechanism 704 through the connecting plate 704. The lead screw mechanism 701 includes a base 701, with upright plates fixedly installed at both ends of the base 701. A lead screw 703 is rotatably installed on the two upright plates. A slide rail 702 is fixedly installed on the base 701 and below the lead screw 703. The lead screw nut 706 is slidably installed on the slide rail 702. One end of the lead screw 703 extends out of either upright plate and is connected to the motor 705 for transmission. It also includes limit sensors 9 for determining the two extreme positions of the lead screw nut 706. The sensing element 901 of the limit sensor 9 is fixed on the lead screw nut 706, and the detection element 902 of the limit sensor 9 is fixed at both ends of the base 701 and is located outside the cuvette station slot 501 at both ends.

[0059] In one embodiment, a light-shielding plate 12 covering a cuvette station slot 501 is fixedly disposed on the connecting plate 704.

[0060] The second drive mechanism is a synchronous belt drive mechanism 8, in which a synchronous slider 22 is fixedly mounted on the synchronous belt, and the synchronous slider 22 is fixedly connected to the transducer 2101. Both the master and slave pulleys of the synchronous belt drive mechanism 8 are rotatably mounted on a bracket, which is fixed to the mounting base 13. The bracket is equipped with a synchronous slide rail that allows the synchronous slider 22 to pass through, ensuring stability during the synchronous belt drive process.

[0061] The third drive mechanism is a linear motor. Linear motors are installed on the Y-axis slide rail 14, X-axis slide rail 16, and Z-axis slide rail 20. The stator of the linear motor is fixed on the corresponding slide rail, and the mover of the linear motor is fixedly connected to the corresponding slider.

[0062] Specifically, the three-axis drive assembly includes Y-axis slide rails 14 symmetrically arranged on the upper part of the chassis 1, Y-axis sliders 15 slidably arranged on both Y-axis slide rails 14, an X-axis slide rail 16 arranged between the two Y-axis sliders 15, an X-axis slider 17 slidably arranged on the X-axis slide rail 16, a Z-axis slide rail 20 fixedly arranged on the X-axis slider 17, a Z-axis slider 19 slidably arranged on the Z-axis slide rail 20, and a pipette 18 fixedly arranged on the Z-axis slider 19; The three-axis drive assembly also includes a drive mechanism three that drives the movement of the Y-axis slider 15, the X-axis slider 17, and the Z-axis slider 19.

[0063] The stator of the linear motor is fixedly mounted, and the mover of the linear motor is fixedly connected to the slider. The slider is slidably mounted on the slide rail. The wavelength switching mechanism and the shared photoelectric receiving module are mounted on the slider. The chassis 1 also includes side panels arranged around the chassis bottom plate 101 and a chassis top plate 102 connecting the four side panels. An L-shaped operating port is opened at the connection between the chassis top plate 102 and the front side panel, and an L-shaped cover is provided on the L-shaped operating port. The upper end of the L-shaped cover is hinged to the chassis top plate 102.

[0064] A transparent observation port is provided on the L-shaped cover, which makes it easy for the operator to observe the detection status inside the chassis 1.

[0065] In one specific embodiment, a controller is also provided inside the chassis 1. The controller can be a microcontroller, a programmable logic controller, an embedded control board, or an industrial control computer. The controller is electrically connected to the pipette 18, drive mechanism 7, drive mechanism 2, drive mechanism 3, servo motor 603, each light source 602, a shared photoelectric receiving module 604, a cleaning water pump 25, a cooling water pump 2302, valves, a transducer 2101, and a cooler. The controller is used to control the pipette 18 to complete tip placement, sample aspiration, reagent aspiration, liquid addition, and waste tip recycling according to the preset detection process; control the servo motor 603 to drive the rotating disk 601 to rotate, so that the target light source 602 enters the detection optical path; control drive mechanism 7 to move the optical detection components to the corresponding cuvette station slot 501; control the shared photoelectric receiving module 604 to collect dark current response, empty cup transmission response, liquid blank reference response, and sample transmission response; and perform dark current subtraction, empty cup ratio compensation, absorbance calculation, and concentration calculation based on the collected photoelectric response data.

[0066] In one specific embodiment, a human-machine interface module can also be installed on the chassis 1. This module is connected to the controller and is used to input the detection items, detection wavelength, reaction time, and sample number. It also displays the detection progress, absorbance, concentration results, abnormal alarm information, and cleaning status. The human-machine interface module may also include a communication interface for uploading the detection results to a host computer.

[0067] How to use: First, place the reagent tubes to be used in the reagent tube rack 201 of reagent storage area 2, place the sample tubes to be tested in the tray 301 of sample storage area 3, place the pipette tip 1801 in the storage box of pipette tip 1801 storage area 4, and place multiple cuvettes in the cuvette slots 501 of cuvette holder 5. The controller calls up the corresponding detection process, target detection wavelength, sample volume, reaction time, and standard curve according to the preset detection items.

[0068] Next, the controller controls the pipette 18 to move along the X, Y, and Z axes, causing the pipette 18 to retrieve the tip from the pipette tip storage area 4 and, according to the detection procedure, to draw the sample to be tested from the sample storage area 3 and the corresponding reagent from the reagent storage area 2, adding the sample and reagent to the corresponding cuvette on the cuvette holder 5. For the cuvette serving as the reference tank, the controller controls the pipette 18 to add the reference solution corresponding to the current detection item. The reference solution can be deionized water and the corresponding colorimetric reagent.

[0069] Before the formal testing, the controller turns off the detection light source 602 or blocks the detection optical path. Dark current response data is collected through the shared photoelectric receiving module 604, and the dark current background value is obtained after abnormal data removal and averaging. .

[0070] Then, the controller determines the target detection wavelength based on the current detection item, and controls the servo motor 603 to drive the rotating disk 601 to rotate, so that the light source 602 corresponding to the target detection wavelength rotates to the detection optical path position and faces the shared photoelectric receiving module 604. Subsequently, the controller controls the drive mechanism to move the optical detection component along the length of the cuvette holder 5, so that the detection optical path passes through the reference slot and each cuvette station slot 501 in sequence.

[0071] With all cuvettes in a dry state (without liquid added), the controller acquires the transmission response value of the empty cuvette in the reference cell via the shared photoelectric receiving module 604. The transmission response values ​​of the empty cups in each cuvette station 501 were collected. For the first... The empty cup transmission response value of the cuvette station 501 is denoted as follows: During the data acquisition process, a preset number of empty cup photoelectric response data can be continuously collected for the same slot. The median is calculated, abnormal data is removed according to a preset empty cup response fluctuation threshold, and the average value of the remaining valid data is calculated to obtain the empty cup transmission response value corresponding to that slot.

[0072] Subsequently, the controller controls the pipette 18 to add reference liquid into the reference tank. After the reference liquid completes the corresponding reaction, the controller controls the optical detection component to move to the corresponding position in the reference tank, and collects the liquid blank reference response value under the target light source 602 through the shared photoelectric receiving module 604. .

[0073] For the Each cuvette station tank 501, the controller responds based on the liquid blank reference value. The empty cup transmission response value of the reference groove , No. The transmission response value En of the empty cup and the dark current background value of the cuvette station 501. Predicting the first The ideal liquid background response value of a cuvette station 501 when filled with reference liquid.

[0074] After the sample completes the corresponding reaction, the controller moves the optical detection component to the next step. The position corresponding to the cuvette station 501 is collected by the shared photoelectric receiving module 604. Transmission response value of sample in cuvette station 501 And based on the ideal liquid background response value Sample transmission response value and dark current background value Calculate the first absorbance of cuvette station 501 Subsequently, the controller determines the absorbance based on... The concentration of the sample to be tested is calculated using a standard curve corresponding to the detection wavelength of the target light source 602.

[0075] When multi-band detection is required, the controller controls the servo motor 603 to drive the rotary disk 601 to rotate again, so that the light source 602 corresponding to the next target detection wavelength enters the detection optical path, and repeats the steps of dark current acquisition, empty cup scanning, liquid blank reference measurement, ideal liquid background prediction, sample transmission response acquisition, absorbance calculation and concentration calculation for the target detection wavelength.

[0076] After the test is completed, the controller can control the ultrasonic cleaning mechanism 21 to move to the corresponding cuvette position, so that the ultrasonic needle 2102 descends into the cuvette. Cleaning water is injected into the cuvette by the cleaning water pump 25, and the transducer 2101 drives the ultrasonic needle 2102 to generate ultrasonic vibration to clean the cuvette. After cleaning, the cleaning solution is discharged through the drain pipe. At the same time, the controller can control the cooling mechanism 23 to cool the reagent storage area 2 to maintain the stability of the reagent storage environment.

[0077] Example 2: This embodiment provides an adaptive compensation method based on an intermittent chemical analyzer, characterized by comprising: S1. Under the light-off state, the dark current background value is collected through the same photoelectric receiving module and recorded as follows: The closed-light state refers to the state when the target light source 602 is not lit, or when the detection light path is blocked.

[0078] S2. Determine the target detection wavelength according to the current detection item, and control the servo multi-wavelength switching mechanism 6 to switch the target detection wavelength to the required light source 602. S3. Select the cuvette station 501 at the starting position as the reference tank. With all cuvettes in the reference tank and each cuvette station 501 in a dry cup state without liquid, drive the optical detection component to move sequentially to the reference tank and each cuvette station 501, and collect the empty cup transmission response value of the reference tank through the same photoelectric receiving module. The transmission response values ​​of the empty cups in each cuvette station 501 were collected; among them, the first... The transmission response value of the empty cup in cuvette station 501 is denoted as: , It is a positive integer; S4. After adding the reference liquid to the reference tank and completing the corresponding reaction, the liquid blank reference response value under the target light source 602 is acquired through the same photoelectric receiving module. ; S5. Based on the liquid blank reference response value The empty cup transmission response value of the reference groove , No. The transmission response value of an empty cup in cuvette station 501 and dark current Predicting the first Ideal liquid background response value of each cuvette station 501 when filled with reference liquid Among them, the ideal liquid background response value Calculate using the following formula:

[0079] S6, To the After the sample to be tested is added to each cuvette station 501 and the corresponding reaction is completed, the transmission response value of the sample is acquired through the same photoelectric receiving module. And based on the ideal liquid background response value Sample transmission response value and the system's dark current background value Calculate the first absorbance of cuvette station 501 Among them, absorbance Calculate using the following formula:

[0080] In the formula, Indicates the first The absorbance of a cuvette station 501 Indicates the first Transmission response value of sample in cuvette station 501; S7. Based on absorbance The concentration of the sample to be tested is obtained by using a standard curve corresponding to the detection wavelength of the target light source 602.

[0081] In step S3, for the same slot, a preset number of empty cup photoelectric response data are continuously collected, the median of the preset number of empty cup photoelectric response data is calculated, abnormal empty cup photoelectric response data are removed based on the median and the preset empty cup response fluctuation threshold, and the average value of the remaining valid empty cup photoelectric response data is calculated to obtain the empty cup transmission response value corresponding to the slot. In step S1, a preset number of dark current response data are continuously collected, the median of the preset number of dark current response data is calculated, abnormal dark current response data are removed based on the median and a preset dark current fluctuation threshold, and the average value of the remaining valid dark current response data is calculated to obtain the dark current background value. .

[0082] Before step S5, it also includes the first step... The transmission response value of an empty cup in cuvette station 501 Transmission response value of empty cup compared with reference groove Compensation ratio between To determine the effectiveness, the compensation ratio is considered. Calculate using the following formula:

[0083] In the formula, Indicates the first The compensation ratio of the empty cup of each cuvette station 501 relative to the reference station; when the compensation ratio When the value exceeds the preset allowable range, the first step is determined. The cuvette corresponding to cuvette station 501 has abnormal light transmission, and the first cuvette... Each cuvette station 501 provides prompts for retesting, rejection, or replacement.

[0084] S8. During multi-wavelength detection, steps S2 to S7 are executed for each target detection wavelength. That is, for each target detection wavelength, the corresponding system dark current background value is obtained. , Reference slot empty cup transmission response value , No. Transmission response value of 501 empty cup in the cuvette station bath Liquid blank reference response value Ideal liquid background response value Sample transmission response value and absorbance The concentration of the corresponding detection parameters is calculated based on the standard curve corresponding to the detection wavelength of each target.

[0085] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A discontinuous chemical analyzer with switchable multi-band light sources, characterized in that, The system includes a chassis, which houses a reagent storage area, a sample storage area, a pipette tip storage area, a cuvette holder, a pipette, and an optical detection assembly. The cuvette holder is provided with a plurality of cuvette slots for placing cuvettes, and each cuvette slot has a light-transmitting hole on its opposite side wall that is in the detection optical path. The pipette is driven by a three-axis drive assembly and is used to transfer liquids and pick up and put down the pipette tip between the reagent storage area, the sample storage area, the pipette tip storage area and the cuvette holder. The optical detection assembly includes a wavelength switching mechanism and a shared photoelectric receiving module. The wavelength switching mechanism includes a rotating disk and a servo motor that drives the rotating disk to rotate. The rotating disk is circumferentially spaced with multiple light sources of different wavelengths. The rotating disk and the shared photoelectric receiving module are located on opposite sides of the cuvette holder. By rotating the rotating disk, any light source can enter the detection position opposite to the shared photoelectric receiving module and form the same detection optical path with the shared photoelectric receiving module and the light-transmitting hole of the corresponding cuvette station slot. The shared photoelectric receiving module is used to receive the detection light signal transmitted through the cuvette.

2. The intermittent chemical analyzer according to claim 1, characterized in that, Multiple cuvette station slots are arranged at intervals along the length of the cuvette support; The optical detection assembly also includes a drive mechanism, which drives the wavelength switching mechanism and the shared photoelectric receiving module to move back and forth synchronously along the length of the cuvette support, so that the detection optical path is aligned with the light-transmitting holes of each cuvette station slot in sequence.

3. The intermittent chemical analyzer according to claim 2, characterized in that, It also includes an ultrasonic cleaning mechanism for cleaning the cuvette; The ultrasonic cleaning mechanism includes a transducer and an ultrasonic needle located below the transducer. The ultrasonic needle has a concentrically arranged channel with an open bottom. The upper part of the ultrasonic needle is provided with a flexible tube connected to the channel. The other end of the flexible tube is connected to the first connection port of a tee. The second connection port of the tee is connected to a cleaning water pump through a water inlet pipe. The cleaning water pump is connected to a cleaning water tank. The third connection port of the tee is connected to a drain pipe. Valves are provided on both the water inlet pipe and the drain pipe. It also includes a second drive mechanism that enables the ultrasonic cleaning mechanism to reciprocate linearly along the Z-axis, and the second drive mechanism is fixed on the output end of the first drive mechanism.

4. The intermittent chemical analyzer according to claim 1, characterized in that, The chassis is provided with a horizontal partition, and the reagent storage area includes a sinkhole provided on the horizontal partition and a reagent tube rack provided in the sinkhole. The bottom surface of the sinking trough is an inclined surface that slopes towards the drain outlet, and a water collection trough is provided on the bottom plate of the chassis and below one side of the drain outlet.

5. The intermittent chemical analyzer according to claim 4, characterized in that, The sample storage area consists of several sample placement slots on the horizontal partition, and samples are placed in the placement slots via trays; the pipette tip storage area consists of mounting slots on the horizontal partition, in which pipette tip storage boxes are placed, and a pipette tip recycling box is provided on the horizontal partition and on one side of the mounting slot.

6. The intermittent chemical analyzer according to claim 4, characterized in that, It also includes a refrigeration mechanism, which includes a cooler, a cooling fan, a hot water exchange tank, a cooling water pump, a circulating water tank, and a heat exchanger; the cooling fan is located on the side of the cooler facing the sinking trough, the hot water exchange tank is located on the other side of the cooler, and the hot water exchange tank, the cooling water pump, the circulating water tank, and the heat exchanger are connected in sequence through pipelines; the sinking trough is arranged adjacent to the cuvette holder.

7. The intermittent chemical analyzer according to claim 3, characterized in that, The three-axis drive assembly includes Y-axis slide rails symmetrically arranged on the upper part of the chassis, Y-axis sliders slidably arranged on both Y-axis slide rails, an X-axis slide rail between the two Y-axis sliders, an X-axis slider slidably arranged on the X-axis slide rail, a Z-axis slide rail fixedly arranged on the X-axis slider, a Z-axis slider slidably arranged on the Z-axis slide rail, and the pipette fixedly arranged on the Z-axis slider. The three-axis drive assembly also includes a drive mechanism three for driving the Y-axis slider, X-axis slider and Z-axis slider.

8. The intermittent chemical analyzer according to claim 7, characterized in that, The drive mechanism one, drive mechanism two, and drive mechanism three can all be one of a lead screw, a linear motor, or a synchronous belt.

9. An adaptive compensation method based on the intermittent chemical analyzer according to any one of claims 1-8, characterized in that, include: S1. Under the light-off state, the dark current background value is collected through the same photoelectric receiving module; S2. Determine the target detection wavelength based on the current detection item, and control the servo multi-wavelength switching mechanism to switch the target detection wavelength to the required light source; S3. Select the cuvette station slot at the starting position as the reference slot. With the cuvettes in the reference slot and each cuvette station slot in a dry cup state without liquid, drive the optical detection component to move sequentially to the reference slot and each cuvette station slot. Collect the empty cup transmission response value of the reference slot through the same photoelectric receiving module, and collect the empty cup transmission response value of each cuvette station slot respectively. S4. After adding reference liquid to the reference tank and completing the corresponding reaction, the liquid blank reference response value under the target light source is collected through the same photoelectric receiving module. S5. Based on the liquid blank reference response value, the empty cup transmission response value of the reference tank, and the first... The empty cup transmission response and dark current of the first cuvette station are used to predict the first... Ideal liquid background response value of each cuvette station tank when filled with reference liquid; S6, To the After the sample to be tested is added to each cuvette station and the corresponding reaction is completed, the sample transmission response value is acquired through the same photoelectric receiving module. The result is then calculated based on the ideal liquid background response value, the sample transmission response value, and the system dark current background value. The absorbance of each cuvette station cell; S7. The concentration of the sample to be tested is obtained from the standard curve corresponding to the absorbance and the detection wavelength of the target light source.

10. The adaptive compensation method according to claim 9, characterized in that, Before step S5, it also includes the first step... The effectiveness of the compensation ratio between the empty cup transmission response value of each cuvette station and the empty cup transmission response value of the reference station is determined.

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