An antibacterial composition applicable to a powder form

Abstract

Disclosed is an antibacterial composition applicable to a powder formulation, which includes a combination of octanediol, ethylhexylglycerin, and p-anisic acid or a combination of 1,3-propanediol, methylpropanediol, and p-anisic acid. The antibacterial composition includes a combination of specific ingredients, thereby being able to exhibit proper preservative capacity at a low content when applied to a powder formulation and not causing mold hyphae or moisture condensation in the powder formulation in a high-humidity environment, thus having excellent stability.

Description

Technical Field

[0001] This relates to an antibacterial composition suitable for powder formulations. Background Technology

[0002] Powder formulations are water-free, thus belonging to a type of cosmetic with a low likelihood of microbial growth. However, if tableted products are transported or stored in high-temperature and high-humidity environments, they provide a suitable surface for microbial growth, and therefore microbial contamination such as mold may occur.

[0003] However, cosmetic preservatives have the characteristic of effectively exhibiting antibacterial activity in water, so the number of antibacterial raw materials suitable for powder is extremely limited, and extremely high concentrations must be used when used alone.

[0004] Therefore, there is a need to develop a preservative material suitable for powder formulations that can effectively inhibit the growth of microorganisms such as bacteria, yeast, and mold in various environments. Summary of the Invention

[0005] Technical issues

[0006] One aspect provides an antibacterial composition suitable for powder formulations, comprising caprylyl glycol, ethylhexylglycerin, and p-anisolic acid.

[0007] On the other hand, an antibacterial composition suitable for powder formulation is provided, comprising 1,3-propanediol, methylpropanediol, and p-anestic acid.

[0008] On the other hand, a cosmetic composition is provided that includes an antibacterial composition suitable for the powder dosage form.

[0009] On the other hand, a topical skin composition is provided, comprising an antibacterial composition suitable for use in the powder formulation.

[0010] On the other hand, a pharmaceutical composition is provided that includes an antibacterial composition suitable for the powder dosage form.

[0011] On the other hand, the use of a combination of caprylyl glycol, ethylhexylglycerin, and p-anestic acid in the preparation of an antibacterial composition suitable for powder formulation is provided.

[0012] On the other hand, the use of a combination of 1,3-propanediol, methylpropanediol, and p-anestic acid in the preparation of an antibacterial composition suitable for powder formulations is provided.

[0013] Technical solution

[0014] On the one hand, an antibacterial composition suitable for powder formulation is provided, comprising caprylyl glycol, ethylhexylglycerin, and p-anisic acid.

[0015] The antibacterial composition comprises a combination of caprylyl glycol, ethylhexylglycerin, and p-anestic acid.

[0016] The weight ratio of caprylyl glycol, ethylhexylglycerin, and p-anisolic acid can be 1 to 32:1 to 16:1 to 32, 1 to 2:1 to 8:1 to 32, 1 to 2:2 to 4:1, 1 to 8:1:1 to 32, 1 to 32:1:1 to 8, 1 to 8:1 to 16:1, 1 to 7:1 to 5:1 to 3, 4 to 6:4 to 6:2 to 4, 4 to 6:4 to 6:3, or 5:5:3. The weight ratio of caprylyl glycol, ethylhexylglycerin, and p-anisolic acid can be any range or value selected from the stated range. When the weight ratio exceeds the stated range, it may exhibit unsuitable preservative properties for powder formulations, or the formulation stability may decrease due to the presence of mold hyphae or water condensation in the powder formulation.

[0017] When the weight ratio of caprylyl glycol, ethylhexylglycerin, and p-anisolic acid is 1 to 2: 1 to 8: 1 to 32, it can be effective against Escherichia coli (E. coli). E. coli It exhibits a synergistic antibacterial effect. When the weight ratio of caprylyl glycol, ethylhexylglycerin, and anisic acid is 1 to 2:2 to 4:1, it can exert a synergistic antibacterial effect against Pseudomonas aeruginosa. P. aeruginosa It exhibits a synergistic antibacterial effect. When the weight ratio of caprylyl glycol, ethylhexylglycerin, and anisic acid is 1 to 8:1:1 to 32, it can exert a synergistic antibacterial effect against Staphylococcus aureus. S. aureus It exhibits a synergistic antibacterial effect. When the weight ratio of caprylyl glycol, ethylhexylglycerin, and anisic acid is 1 to 32:1:1 to 8, it can exert an antibacterial effect against yeast (…). C. albicans It exhibits a synergistic antibacterial effect. When the weight ratio of caprylyl glycol, ethylhexylglycerin, and p-anisolic acid is 1 to 8:1 to 16:1, it can exert an antibacterial effect against molds ( A. brasiliensis It exhibits a synergistic antibacterial effect.

[0018] In one specific embodiment, the content of octyl glycol relative to the total weight of the composition may be 0.004 wt% to 5 wt%, 0.004 wt% to 3 wt%, 0.004 wt% to 1 wt%, 0.004 wt% to 0.7 wt%, 0.004 wt% to 0.6 wt%, 0.04 wt% to 5 wt%, 0.04 wt% to 3 wt%, 0.04 wt% to 1 wt%, 0.04 wt% to 0.7 wt%, 0.04 wt% to 0.6 wt%, 0.1 wt% to 5 wt%, 0.1 wt% to 3 wt%, 0.1 wt% to 1 wt%, 0.1 wt% to 0.7 wt%, 0.1 wt% to 0.6 wt%, 0.1 wt% to 0.5 wt%, 0.1 wt% to 0.4 wt%, and 0.2 wt% to 0.7 wt%. wt%, 0.2 wt% to 0.6 wt%, 0.2 wt% to 0.5 wt%, 0.2 wt% to 0.4 wt%, 0.3 wt% to 0.7 wt%, 0.3 wt% to 0.6 wt%, 0.3 wt% to 0.5 wt%, 0.3 wt% to 0.4 wt%, 0.4 wt% to 0.7 wt%, or 0.4 wt% to 0.6 wt%. When the content of caprylyl glycol exceeds the above range, problems such as microbial contamination due to reduced antimicrobial synergy between preservative raw materials or skin irritation due to excessive preservative content may occur.

[0019] In one specific embodiment, the content of ethylhexylglycerin relative to the total weight of the composition may be 0.004 wt% to 5 wt%, 0.004 wt% to 3 wt%, 0.004 wt% to 1 wt%, 0.004 wt% to 0.7 wt%, 0.004 wt% to 0.6 wt%, 0.04 wt% to 5 wt%, 0.04 wt% to 3 wt%, 0.04 wt% to 1 wt%, 0.04 wt% to 0.7 wt%, 0.04 wt% to 0.6 wt%, 0.1 wt% to 5 wt%, 0.1 wt% to 3 wt%, 0.1 wt% to 1 wt%, 0.1 wt% to 0.7 wt%, 0.1 wt% to 0.6 wt%, 0.1 wt% to 0.5 wt%, 0.1 wt% to 0.4 wt%, and 0.2 wt% to 0.7 wt%. wt%, 0.2 wt% to 0.6 wt%, 0.2 wt% to 0.5 wt%, 0.2 wt% to 0.4 wt%, 0.3 wt% to 0.7 wt%, 0.3 wt% to 0.6 wt%, 0.3 wt% to 0.5 wt%, 0.3 wt% to 0.4 wt%, 0.4 wt% to 0.7 wt%, or 0.4 wt% to 0.6 wt%. When the content of ethylhexylglycerin exceeds the above range, problems such as microbial contamination due to reduced antimicrobial synergy between preservatives or skin irritation due to excessive preservative content may occur.

[0020] In one specific embodiment, the content of p-anesic acid relative to the total weight of the composition may be 0.02 wt% to 0.5 wt%, 0.02 wt% to 0.4 wt%, 0.02 wt% to 0.3 wt%, 0.1 wt% to 0.5 wt%, 0.1 wt% to 0.4 wt%, 0.1 wt% to 0.3 wt%, 0.2 wt% to 0.5 wt%, 0.2 wt% to 0.4 wt%, or 0.2 wt% to 0.3 wt%. When the content of p-anesic acid exceeds the aforementioned range, problems may arise such as microbial contamination due to reduced antimicrobial synergy between preservative raw materials, skin irritation due to excessive preservative content, or decreased dosage form stability due to raw material precipitation.

[0021] When the antimicrobial composition is applicable to a powder formulation composition (e.g., a cosmetic composition), the content of each of caprylyl glycol, ethylhexylglycerin, and anisic acid may be relative to the total weight of the powder formulation composition.

[0022] On the other hand, an antibacterial composition suitable for powder formulation is provided, comprising 1,3-propanediol, methylpropanediol, and p-anisic acid.

[0023] The antibacterial composition comprises a combination of 1,3-propanediol, methylpropanediol, and p-anestic acid.

[0024] The weight ratio of 1,3-propanediol, methylpropanediol, and p-anesic acid can be 1 to 512:1 to 512:1, 2 to 128:1 to 32:1, 8 to 32:32 to 64:1, 1 to 128:1 to 128:1, 4 to 512:4 to 512:1, 8 to 256:4 to 256:1, 5 to 10:5 to 10:1 to 3, 4 to 6:9 to 11:2 to 4, 4 to 6:9 to 11:3, or 5:10:3. The weight ratio of 1,3-propanediol, methylpropanediol, and p-anesic acid can be any range or value selected from the stated range. When the weight ratio exceeds the stated range, it may exhibit unsuitable preservative properties for powder formulations, or the formulation stability may decrease due to the presence of mold hyphae or water condensation in the powder formulation.

[0025] When the weight ratio of 1,3-propanediol, methylpropanediol, and p-anisic acid is 2 to 128:1 to 32:1, it can be effective against Escherichia coli (E. coli). E. coli It exhibits a synergistic antibacterial effect. When the weight ratio of 1,3-propanediol, methylpropanediol, and p-anisolic acid is 8 to 32:32 to 64:1, it can exert a synergistic antibacterial effect against Pseudomonas aeruginosa. P. aeruginosa It exhibits a synergistic antibacterial effect. When the weight ratio of 1,3-propanediol, methylpropanediol, and anisic acid is 1 to 128:1 to 128:1, it can inhibit Staphylococcus aureus (…). S. aureus It exhibits a synergistic antibacterial effect. When the weight ratio of 1,3-propanediol, methylpropanediol, and anisic acid is 4 to 512:4 to 512:1, it can exert an antibacterial effect against yeast ( C.albicans It exhibits a synergistic antibacterial effect. When the weight ratio of 1,3-propanediol, methylpropanediol, and p-anestic acid is 8 to 256:4 to 256:1, it can exert an antibacterial effect against molds ( A. brasiliensis It exhibits a synergistic antibacterial effect.

[0026] In one specific embodiment, the content of 1,3-propanediol relative to the total weight of the composition may be 0.25 wt% to 32 wt%, 0.25 wt% to 16 wt%, 0.25 wt% to 8 wt%, 0.25 wt% to 4 wt%, 0.25 wt% to 2 wt%, 0.25 wt% to 1 wt%, 0.25 wt% to 0.8 wt%, 0.25 wt% to 0.5 wt%, 0.3 wt% to 32 wt%, 0.3 wt% to 16 wt%, 0.3 wt% to 8 wt%, 0.3 wt% to 4 wt%, 0.3 wt% to 2 wt%, 0.3 wt% to 1 wt%, 0.3 wt% to 0.8 wt%, 0.3 wt% to 0.5 wt%, 0.35 wt% to 32 wt%, 0.35 wt% to 16 wt%. wt%, 0.35 wt% to 8 wt%, 0.35 wt% to 4 wt%, 0.35 wt% to 2 wt%, 0.35 wt% to 1 wt%, 0.35 wt% to 0.8 wt%, or 0.35 wt% to 0.5 wt%. When the content of 1,3-propanediol exceeds the range described above, problems such as microbial contamination due to reduced antimicrobial synergy between preservative raw materials or skin irritation due to excessive preservative content may occur.

[0027] In one specific embodiment, the content of methylpropanediol relative to the total weight of the composition may be 0.25 wt% to 32 wt%, 0.25 wt% to 16 wt%, 0.25 wt% to 8 wt%, 0.25 wt% to 4 wt%, 0.25 wt% to 2 wt%, 0.25 wt% to 1.5 wt%, 0.25 wt% to 1 wt%, 0.5 wt% to 32 wt%, 0.5 wt% to 16 wt%, 0.5 wt% to 8 wt%, 0.5 wt% to 4 wt%, 0.5 wt% to 2 wt%, 0.5 wt% to 1.5 wt%, 0.5 wt% to 1 wt%, 0.7 wt% to 32 wt%, 0.7 wt% to 16 wt%, 0.7 wt% to 8 wt%, 0.7 wt% to 4 wt%, 0.7 wt% to 2 wt%, 0.7 wt% to 0.7 wt% The concentration of methyl propylene glycol may be 1.5 wt% to 1.5 wt% or 0.7 wt% to 1 wt%. When the concentration of methyl propylene glycol exceeds the range described above, problems such as microbial contamination due to reduced antimicrobial synergy between preservatives or skin irritation due to excessive preservative concentration may occur.

[0028] In one specific embodiment, the content of p-anesic acid relative to the total weight of the composition may be 0.02 wt% to 0.5 wt%, 0.02 wt% to 0.4 wt%, 0.02 wt% to 0.3 wt%, 0.1 wt% to 0.5 wt%, 0.1 wt% to 0.4 wt%, 0.1 wt% to 0.3 wt%, 0.2 wt% to 0.5 wt%, 0.2 wt% to 0.4 wt%, or 0.2 wt% to 0.3 wt%. When the content of p-anesic acid exceeds the aforementioned range, problems may arise such as microbial contamination due to reduced antimicrobial synergy between preservative raw materials, skin irritation due to excessive preservative content, or decreased dosage form stability due to raw material precipitation.

[0029] When the antimicrobial composition is applicable to a powder formulation composition (e.g., a cosmetic composition), the content of each of 1,3-propanediol, methylpropanediol, and anisic acid may be relative to the total weight of the powder formulation composition.

[0030] According to the above aspects, the antimicrobial composition is suitable for powder formulations. "Applicable to powder formulation" can mean that it can be used as a preservative in the final powder formulation product. Unlike conventional cosmetic preservatives designed for aqueous formulations, the antimicrobial composition according to the above aspects is suitable for anhydrous powder formulations. According to the above aspects, the antimicrobial composition comprises a combination of three specific ingredients, thus exhibiting suitable preservative capabilities for powder formulations even at low concentrations. Furthermore, even when transporting or storing powder formulation products using the antimicrobial composition according to the above aspects under high temperature and humidity conditions, mold hyphae or water condensation will not occur.

[0031] In this specification, the term "antimicrobial composition" refers to a composition capable of killing microorganisms (e.g., bacteria, yeast, mold, etc.) or inhibiting their growth, and this term may be used interchangeably with "preservative composition" or "antiseptic composition".

[0032] According to the aforementioned aspects, the antimicrobial composition may exhibit antimicrobial activity against one or more selected from bacteria, yeasts, and molds.

[0033] The bacteria may include those selected from Escherichia coli (Escherichia coli) Escherichia coli ), Pseudomonas aeruginosa ( Pseudomonas aeruginosa ) and Staphylococcus aureus ( Staphylococcus aureus One or more of these, but not limited to them.

[0034] The yeast may include Candida albicans ( Candida albicans (but not limited to this).

[0035] The mold may include Aspergillus brasiliensis ( Aspergillus brasiliensis (but not limited to this).

[0036] Even when transporting or storing powder formulations of the antimicrobial composition according to the above-described aspects under high temperature and humidity conditions, no mold hyphae or water condensation may occur. For example, the high temperature and humidity environment may refer to relative humidity greater than or equal to 40%, 50%, 60%, 70%, 80%, or 90%, and temperature conditions greater than or equal to 25°C, 30°C, 32°C, 35°C, or 25°C to 40°C. In one embodiment, for a powder formulation cosmetic composition of the antimicrobial composition according to the above-described aspects, no mold hyphae or water condensation occurred when incubated for 28 days at 95% relative humidity (room temperature 25°C or high temperature 32°C).

[0037] On the other hand, a cosmetic composition is provided, comprising an antibacterial composition suitable for powder formulation according to said aspect.

[0038] The cosmetic composition is not limited to a specific dosage form, and the dosage form can be appropriately selected according to the purpose. In one specific embodiment, the cosmetic composition may be a powder dosage form.

[0039] The cosmetic composition may include conventionally used ingredients, functional additives, etc., such as conventional adjuvants such as antioxidants, stabilizers, vitamins, pigments, and fragrances, as well as carriers.

[0040] On the other hand, a topical skin composition is provided, comprising an antibacterial composition suitable for powder formulation according to said aspect.

[0041] In one specific embodiment, the topical skin composition may be in powder form.

[0042] The aforementioned topical skin agent may be mixed with ingredients commonly used in cosmetics, pharmaceuticals, quasi-pharmaceuticals, etc., as needed, such as powder ingredients, ultraviolet absorbers, whitening agents, antioxidants, fragrances, colorants, various skin nutrients, metal ion chelating agents, sugars, or combinations thereof.

[0043] On the other hand, a pharmaceutical composition is provided, comprising an antibacterial composition suitable for powder dosage forms according to said aspect.

[0044] The antibacterial composition can be used as an active ingredient in a pharmaceutical composition with antibacterial activity, or as an adjuvant to an active ingredient with pharmaceutical use.

[0045] In one specific embodiment, the pharmaceutical composition may be in powder form.

[0046] On the other hand, the use of a combination of caprylyl glycol, ethylhexylglycerin, and p-anestic acid in the preparation of an antibacterial composition suitable for powder formulation is provided.

[0047] On the other hand, the use of a combination of 1,3-propanediol, methylpropanediol, and p-anestic acid in the preparation of an antibacterial composition suitable for powder formulations is provided.

[0048] Given the complexity of this specification, repetitive content has been omitted. Terms not defined in this specification have the same meaning as those conventionally used in the relevant technical field.

[0049] Beneficial effects

[0050] According to one aspect, the antibacterial composition comprises a combination of caprylyl glycol, ethylhexylglycerin, and p-anisolic acid, or a combination of 1,3-propanediol, methylpropanediol, and p-anisolic acid. This allows it to exhibit appropriate preservative properties even at low concentrations when applied to powder formulations, and it does not cause mold hyphae or moisture condensation in the powder formulation under high humidity conditions, thus exhibiting excellent stability. Therefore, the antibacterial composition can be used as a preservative for powder-based cosmetics, topical skin agents, etc. Attached Figure Description

[0051] Figure 1 The results show the incubation of a powder formulation (sample number 3-2) of caprylyl glycol, ethylhexylglycerin, and anisic acid, which are suitable as antibacterial raw materials, under high humidity conditions.

[0052] Figure 2 The results show the incubation of a powder formulation (sample number 4-3) of a combination of 1,3-propanediol and methylpropanediol, which is suitable as an antibacterial raw material, under high humidity conditions.

[0053] Figure 3 The results show the incubation of a powder formulation (sample number 4-4) of a combination of 1,3-propanediol and methylpropanediol, which is suitable as an antibacterial raw material, under high humidity conditions.

[0054] Figure 4 The results show the incubation of powder formulations (samples 4-5) of a combination of 1,3-propanediol and methylpropanediol, which are suitable as antibacterial raw materials, under high humidity conditions.

[0055] Figure 5The results show the incubation of a powder formulation (sample number 5-2) of 1,3-propanediol, methylpropanediol, and anisic acid, which are suitable as antibacterial raw materials, under high humidity conditions. Detailed Implementation

[0056] The following is a detailed description with reference to embodiments. However, these embodiments are intended to exemplify one or more specific implementations, and the scope of the invention is not limited thereto.

[0057] Example 1: Confirmation of antibacterial synergistic effect

[0058] The three-dimensional antimicrobial synergistic effect of the combination of caprylyl glycol, ethylhexylglycerin, and p-anesic acid, and the combination of 1,3-propanediol, methylpropanediol, and p-anesic acid were confirmed. The specific testing methods for the antimicrobial synergistic effect are as follows. The concentration of the raw materials was based on the minimum inhibitory concentration (MIC) of each raw material.

[0059] In bacterial tests, dilutions were performed using Tryptic Soy Agar (TSA), and in fungal tests, dilutions were performed using Sabouraud Dextrose Agar (SDA) to the desired concentration. To determine the minimum inhibitory concentration (MIC) of the raw material, 100 µl of adjusted bacterial suspension was added to each well of a 96-well plate. In the first row of the plate containing 100 µl of bacterial suspension per well, 100 µl of the prepared raw material was added to each well, and the bacterial suspension and raw material were mixed thoroughly. In the second row, 100 µl of the mixture was added to each well to dilute the raw material by half. The raw material was serially diluted by half each time, and the remaining 100 µl of mixture in the last row was discarded. After incubation, the turbidity of the medium was checked to visually determine whether bacteria had grown. Based on the turbidity, the lowest concentration of no-growth bacteria was taken as the MIC of the raw material. The minimum inhibitory concentration (wt%) of raw materials for each strain measured in this test is shown in Table 1.

[0060] The ingredients A (caprylyl glycol or 1,3-propanediol), B (ethylhexylglycerol or methylpropanediol), and C (p-anestic acid), whose antibacterial synergistic effects were to be determined, were prepared at 8-fold minimum inhibitory concentrations (MICs). Using tryptone-soy agar medium, the prepared ingredients were serially diluted by half each time, thus preparing diluted ingredients A and B in the range of 8 to 1 / 8 of the MIC. Diluted ingredients C in the range of 8 to 1 times the MIC were also prepared.

[0061] For type A raw materials, aliquots were dispensed into the wells of the X-axis row of a 96-well plate at 25 µl per well, in descending order of concentration (1 / 8 of the minimum inhibitory concentration, MIB) to high concentration (8 times the MIB). For type B raw materials, aliquots were dispensed into the wells of the Y-axis row at 25 µl per well, in descending order of concentration (1 / 8 of the MIB) to high concentration (8 times the MIB). For type C raw materials, aliquots were dispensed into the wells of the Z-axis plate of a 96-well plate at 25 µl per well, in descending order of concentration (8 times the MIB). 25 µl of each bacterial culture for adjusting bacterial count was dispensed into each well containing the raw materials and incubated.

[0062] The product contains E. coli ( E. coli ), Pseudomonas aeruginosa ( P. aeruginosa Staphylococcus aureus ( S. aureus The strain and diluted raw material were placed in a 96-well plate at 32°C and incubated for 24 hours. The plates containing Candida albicans (…) were then aliquoted… C. albicans Aspergillus brasiliensis ( ) A. brasiliensis The strain and diluted raw materials were placed in a 96-well plate at 25°C and incubated for 48 hours. Then the mixing ratio (by weight) at which the lowest concentration of bacterial growth was not visually observed was determined.

[0063] To confirm the most effective mixed concentration results, the fractional inhibiting concentration (FIC) and FIC index were calculated by substituting into the following mathematical formula.

[0064]

Mathematical Formula 1

[0065] The FIC of substance A, B, or C = (MIC of raw material A, B, or C in the mixture) / (MIC when raw material A, B, or C is used alone)

[0066]

Mathematical Formula 2

[0067] FIC index = FIC of substance A + FIC of substance B + FIC of substance C

[0068] If no bacterial proliferation is observed within the range of FIC index ≤ 0.5, it is considered to have a complete synergistic effect; if it is 0.5 < FIC index ≤ 1, it is considered to have a partial synergistic effect; if it is 1 < FIC index ≤ 4, it is considered to be indifferent; and if it is FIC index > 4, it is considered to have an antagonistic effect.

[0069] In this test, based on the minimum inhibitory concentration (MIC) of each ingredient, the dosage of caprylyl glycol was 0.004 wt% to 0.5 wt%, ethylhexylglycerin was 0.004 wt% to 1 wt%, 1,3-propanediol and methylpropanediol were 0.25 wt% to 32 wt%, and p-anisic acid was 0.02 wt% to 0.5 wt%. Table 2 below shows the antimicrobial synergistic effect test results of the combination of caprylyl glycol, ethylhexylglycerin, and p-anisic acid with the combination of 1,3-propanediol, methylpropanediol, and p-anisic acid, expressed as weight ratios indicating synergistic effects between ingredients.

[0070] <Abbreviations>

[0071] CG: Caprylyl glycol

[0072] EHG: Ethylhexylglycerin

[0073] p-AA: p-Anisic acid

[0074] 1,3-PD: 1,3-Propanediol

[0075] MPD: Methylpropanediol

[0076] Table 1

[0077] Table 2

[0078] As shown in Table 2 above, the results of the antibacterial synergistic effect test on bacteria indicate that the combination of caprylyl glycol, ethylhexylglycerin, and anisic acid in a weight ratio of 1 to 2:1 to 8:1 to 32 is effective against Escherichia coli (E. coli). E. coli It exhibits synergistic antibacterial activity against Pseudomonas aeruginosa at a weight ratio of 1 to 2:2 to 4:1. P. aeruginosa It exhibits synergistic antibacterial activity against Staphylococcus aureus at weight ratios of 1 to 8:1:1 to 32. S. aureus It exhibits synergistic antibacterial activity. At weight ratios of 1 to 32:1:1 to 8, it shows antibacterial activity against yeast (…). C. albicans It exhibits synergistic antibacterial activity. At weight ratios of 1 to 8:1 to 16:1 to 16, it shows antimicrobial synergistic effects against molds (…). A. brasiliensisIt exhibits a synergistic antibacterial effect. Regarding the combination of 1,3-propanediol, methylpropanediol, and anisic acid, at weight ratios of 2 to 128:1 to 32:1, it shows a synergistic antibacterial effect against Escherichia coli (E. coli). E. coli It exhibited synergistic antibacterial activity against Pseudomonas aeruginosa at weight ratios of 8 to 32:32 to 64:1. P. aeruginosa It exhibits synergistic antibacterial activity against Staphylococcus aureus at weight ratios of 1 to 128:1 to 128:1. S. aureus It exhibits synergistic antimicrobial activity. It shows synergistic antimicrobial activity against yeast at a weight ratio of 4 to 512:4 to 512:1. It also shows synergistic antimicrobial activity against mold at a weight ratio of 8 to 256:4 to 256:1.

[0079] Example 2: Corrosion Resistance Test

[0080] The preservative properties of powder-form cosmetic compositions containing antibacterial compositions were tested.

[0081] First, a cosmetic composition in powder form containing the antibacterial ingredients (wt%) listed in Table 3 below was prepared. The cosmetic composition in powder form was prepared by the following conventional method: pulverizing the powder to prepare a powder form, placing it in a Henschel mixer, spraying an oily binder into the Henschel mixer, mixing, and then compressing. Specifically, 70 wt% to 80 wt% of the powder base and 5 wt% to 10 wt% of the pigment were placed in a mixer and mixed at 1200 rpm to 1500 rpm for 1 to 2 minutes. Then, 10 wt% to 20 wt% of the oily binder was heated to 65°C to 85°C and mixed, while being sprayed directly onto the pre-mixed powder form while stirring at 200 rpm to 400 rpm. The powder substrate is composed of one or more of the following: powdered talc or titanium dioxide, mica, silica, nylon powder, aluminum starch octenyl succinate, magnesium myristate, magnesium stearate, lauroyl lysine, triethoxyoctylsilane, and p-anisolic acid. The binder includes one or more of the following: oily caprylic / capric triglyceride, triethylhexanoate, polyglycerol-2 triisostearate, hydrogenated castor oil isostearate, caprylyl glycol, ethylhexylglycerin, propylene glycol, and methyl propylene glycol. The pigments include one or more of the following: yellow iron oxide, red iron oxide, black iron oxide, selenide sulfide, ferrocyanide, carmine, and manganese violet.

[0082] Next, the preservative properties of the powder-form cosmetic composition prepared above were tested. Specifically, the preservative properties of Escherichia coli (E. coli) were tested. Escherichia coli ATCC 8739, Pseudomonas aeruginosa ( Pseudomonas aeruginosa ATCC 9027 and Staphylococcus aureus ( Staphylococcus aureusATCC 6538; Candida albicans in yeast ( Candida albicans ATCC 10231; and Aspergillus brasiliensis among molds ( Aspergillus brasiliensis The test was conducted according to ATCC 16404. Using 0.85% NaCl, the bacterial suspensions (Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 9027, and Staphylococcus aureus ATCC 6538) were adjusted and mixed until each bacterium count reached 10-1. 8 CFU / ml, adjust the yeast (Candida albicans ATCC 10231) and mold (Aspergillus brasiliensis ATCC 16404) to a count of 10 for each. 7 CFU / ml. 1% bacterial, yeast, and mold cultures were added to each of the prepared 20 g powder formulations and mixed. The mixture was then incubated at 25°C for 4 weeks. The remaining bacterial count was measured at weeks 1, 2, and 4, and the bacterial reduction rate was confirmed by converting the values ​​to logarithmic values.

[0083] The corrosion resistance test results are shown in Table 3 below.

[0084] <Abbreviations>

[0085] CG: Caprylyl glycol

[0086] EHG: Ethylhexylglycerin

[0087] p-AA: p-Anisic acid

[0088] 1,3-PD: 1,3-Propanediol

[0089] MPD: Methylpropanediol

[0090] Table 3

[0091] As shown in Table 3 above, anisic acid (samples 1-1 to 1-3) or the combination of caprylyl glycol and ethylhexylglycerin (samples 2-1 to 2-3) exhibited unsuitable preservative properties in powder formulations. The combination of 1,3-propanediol and methylpropanediol (samples 4-1 and 4-2) exhibited suitable preservative properties in powder formulations, but as shown in Example 3 below, mold hyphae or moisture condensation occurred in the powder formulations under high humidity conditions, thus reducing stability.

[0092] Conversely, the combination of caprylyl glycol, ethylhexylglycerin and p-anisolic acid (sample number 3-1) or the combination of 1,3-propanediol, methylpropanediol and p-anisolic acid (sample number 5-1) also showed suitable preservative properties for powder formulations at low concentrations.

[0093] Example 3: Mold test and stability assessment

[0094] Mold testing and stability evaluation were conducted on powder-form cosmetic compositions containing antibacterial compositions.

[0095] First, a cosmetic composition in powder form containing the antibacterial components (wt%) listed in Table 4 was prepared. The method for preparing the powder-form cosmetic composition was the same as in Example 2.

[0096] Next, the prepared powder-form cosmetic composition was subjected to mold testing and stability evaluation. Specifically, 3 μL of adjusted 10 μL was inoculated into one or more portions of the compressed powder-form cosmetic composition. 6 The mold culture was incubated at CFU / mL. After incubation for 28 days under the conditions shown in Table 3 below, it was confirmed whether mold mycelia and water condensation occurred.

[0097] Visual inspection is used to confirm the presence of mold hyphae. The results are indicated by the degree of presence as follows: -: No hyphae are present at the inoculation site; +: No hyphae are observed, but spores are spreading around the inoculation site; ++: A small number of hyphae are observed around the inoculation site, and spores are spreading; +++: A large number of hyphae are observed around the inoculation site.

[0098] Condensation is visually inspected to confirm its presence. If condensation is observed, the system is deemed unqualified in the stability test.

[0099] Table 4

[0100] As shown in Table 4 above and Figures 1 to 5 As shown, the combination of 1,3-propanediol and methylpropanediol (samples 4-3 to 4-5) exhibited suitable preservative properties for powder formulations, but mold mycelia or moisture condensation occurred under high humidity conditions, indicating low safety and stability. In contrast, the combination of caprylyl glycol, ethylhexylglycerin, and p-anesic acid (sample 3-2) or the combination of 1,3-propanediol, methylpropanediol, and p-anesic acid (sample 5-2) did not exhibit mold mycelia or moisture condensation under high humidity conditions, thus confirming their excellent safety and stability.

Claims

1. An antibacterial composition suitable for powder formulations, comprising: Caprylyl glycol, ethylhexylglycerin, and p-anestic acid.

2. The antibacterial composition applicable to powder formulations according to claim 1, wherein, The weight ratio of caprylyl glycol, ethylhexylglycerin, and p-anestic acid is 1 to 32: 1 to 16: 1 to 32.

3. The antibacterial composition applicable to powder formulations according to claim 1, wherein, Based on the total weight of the composition, The content of octyl glycol is from 0.004 wt% to 5 wt%. The content of the ethylhexylglycerol is from 0.004 wt% to 5 wt%. The content of the anisolic acid is from 0.02 wt% to 0.5 wt%.

4. An antibacterial composition suitable for powder formulations, comprising: 1,3-Propanediol, methylpropanediol, and p-anestic acid.

5. The antibacterial composition applicable to powder formulations according to claim 4, wherein, The weight ratio of 1,3-propanediol, methylpropanediol, and p-anestic acid is 1 to 512:1 to 512:

1.

6. The antibacterial composition applicable to powder formulations according to claim 4, wherein, Based on the total weight of the composition, The content of the 1,3-propanediol is from 0.25 wt% to 32 wt%. The content of methylpropanediol is from 0.25 wt% to 32 wt%. The content of the anisolic acid is from 0.02 wt% to 0.5 wt%.

7. The antibacterial composition applicable to powder formulations according to claim 1 or claim 4, wherein, It exhibits antibacterial activity against one or more of bacteria, yeasts, and molds.

8. The antibacterial composition applicable to powder formulations according to claim 7, wherein, The bacteria include those selected from Escherichia coli (Escherichia coli) Escherichia coli ), Pseudomonas aeruginosa ( Pseudomonas aeruginosa ) and Staphylococcus aureus ( Staphylococcus aureus One or more of the following, The yeast includes Candida albicans ( Candida albicans ), The mold includes Aspergillus brasiliensis ( Aspergillus brasiliensis ).

9. A cosmetic composition comprising: The antibacterial composition of claim 1 or claim 4 is applicable to powder formulations.

10. A topical skin composition comprising: The antibacterial composition of claim 1 or claim 4 is applicable to powder formulations.

Images