An intelligent system and method for the whole process of asexual tissue culture propagation of arundo donax

Abstract

The application is a whole-process intelligent system and method for asexual tissue culture propagation of reed, which comprises a data acquisition and execution layer of an integrated intelligent unmanned aerial vehicle platform, a molecular biology laboratory, an intelligent disinfection inoculation room, a material storage and transfer unit, a seedling cultivation unit, a three-level biological seed source library, a cleaning and disinfection and culture medium workshop, a tissue culture seedling transplanting room, an intelligent planting transplanting machine, an intelligent field equipment, an intelligent harvester and a wind power photovoltaic intelligent charging and battery swapping nest station; a plurality of edge cloud brains are deployed to realize edge computing and decision-making layer for local real-time control; a cloud data processing and storage layer is constructed by gathering multi-source data through a supercomputing data center, running an AI model and constructing an unalterable whole-life-cycle data asset based on a block chain. The application constructs a whole-life-cycle intelligent system for reed from seed selection and breeding to plant protection and harvesting, which is suitable for large-scale and standardized intelligent planting of reed.

Description

Technical Field

[0001] This invention relates to the fields of plant breeding engineering and agricultural planting technology, and in particular to an intelligent system and method for the entire process of asexual tissue culture propagation of Reed spp. Background Technology

[0002] Reed (Phragmites australis), a clonal grass native to China, possesses characteristics such as salt and alkali resistance, drought resistance, cold tolerance, high yield, and high carbon sequestration. A single planting can yield continuous harvests for 15-20 years, making it a strategically valuable energy plant and industrial biomass raw material with broad application prospects in ecological restoration, biomass energy, and pulp raw materials. However, the breeding and cultivation of Reed plants currently still face the following common bottlenecks:

[0003] The selection, breeding, and seedling production cycle is long: relying on traditional hybridization and phenotypic selection takes many years and cannot meet the rapid market demand.

[0004] It relies heavily on human experience: There are many manual processes (such as seed selection, disinfection, inoculation, plant protection, etc.), the standardization is low, it is easily affected by subjective factors, and manual operation can easily cause seedling contamination.

[0005] Environmental constraints: Uncontrollable factors such as climate and soil lead to poor seedling stability and make large-scale production difficult.

[0006] Data fragmentation: Data from seed selection, breeding, seedling raising, and planting are scattered and lack full life-cycle data linkage analysis, making it difficult to support accurate decision-making.

[0007] In existing technologies, traditional breeding and planting rely entirely on manual observation, resulting in low data collection efficiency and large errors. Localized intelligent applications, such as agricultural IoT, are mostly limited to single stages (e.g., irrigation, temperature control), failing to cover the entire chain of "seed selection-breeding-seedling raising-planting." Biotechnologies such as molecular marker-assisted selection still rely on laboratory operations, disconnected from field management and failing to form a closed-loop optimization. Therefore, there is an urgent need for a fully intelligent system driven by multi-dimensional data from "genotype-phenotype-environment" to improve the efficiency of Reed Bamboo breeding and planting, and to achieve a replicable and scalable standardized production model. Summary of the Invention

[0008] This invention aims to address the shortcomings of existing technologies by providing an intelligent system and method for the entire process of asexual tissue culture propagation of Reed spp., achieving a closed loop of "perception-decision-execution", shortening the breeding cycle, improving seedling survival rate, and building traceable data assets.

[0009] To achieve the above objectives, the present invention adopts the following technical solution: an intelligent system for the entire process of asexual tissue culture propagation of Reed japonica plants, comprising:

[0010] The data acquisition and execution layer includes:

[0011] Intelligent drone platform for collecting phenotypic data of Reed hyacinth plants and performing plant protection and field management operations;

[0012] The molecular biology laboratory is equipped with automated nucleic acid extraction devices, high-throughput gene sequencing devices, and bioinformatics analysis software systems to obtain plant genotype data, establish DNA molecular marker maps, realize molecular marker-assisted selection, and mark the source of seed by region, block, location, time, environment, and batch.

[0013] The intelligent disinfection and inoculation room is used for automated disinfection and pruning inoculation of seedlings;

[0014] The material storage and transfer unit, including an external seed bank and intelligent AGV carts, is used for storing materials and transferring them between units.

[0015] The seedling cultivation unit includes a constant temperature and humidity induction culture room, a constant temperature and humidity rooting culture room, a greenhouse nursery, and a sunroom, which are used for intelligent seedling cultivation.

[0016] A three-tiered biological germplasm bank is used for the graded preservation of germplasm resources.

[0017] Cleaning and disinfection and culture medium workshop, used for cleaning and disinfecting culture containers and preparing culture media;

[0018] The tissue culture seedling transplanting room is used to harden off tissue culture seedlings and transplant them to the greenhouse nursery.

[0019] Intelligent planting and transplanting machine is used to automatically transplant seedlings from seedling trays to the field;

[0020] Intelligent field equipment includes intelligent weather stations, soil sensors, water and fertilizer irrigation systems, robotic dogs for inter-row operations and sampling, and sampling and transportation drones;

[0021] Intelligent harvesters are used for the automated harvesting of mature reeds.

[0022] The smart charging and swapping station for wind and solar power integrates an RTK base station and an edge cloud brain to provide charging and swapping services and positioning references for field equipment;

[0023] The edge computing and decision-making layer includes multiple edge cloud brains deployed in each key functional unit. Each edge cloud brain is used to receive and process real-time data from the data acquisition and execution layer, execute local decisions, and control devices in its assigned area.

[0024] The cloud-based data processing and storage layer includes a supercomputing data center for breeding, seedling cultivation, and planting. This supercomputing data center communicates with various edge cloud brains to aggregate multi-source data, run AI algorithm models and knowledge graph prediction model libraries, and, through blockchain technology, form a distributed encrypted storage with various Chinese herbal medicine planting and breeding alliance chain data centers, various energy biomass alliance chain data centers, and various raw material biomass alliance chain data centers to build tamper-proof, full-lifecycle data assets.

[0025] In particular, the intelligent drone platform is equipped with multispectral / hyperspectral lidar, air composition detection sensors, Beidou precise positioning system, geographic information electronic map, high-definition camera and identification system with infrared night vision function, and satellite 5G communication system, which are used to automatically collect phenotypic data of Reed Plant height, diameter, crown width, biomass, chlorophyll content, nitrogen status, carbon dioxide and oxygen concentration over a large area and with high throughput.

[0026] In particular, the intelligent disinfection and inoculation room is equipped with an indoor temperature and humidity and negative pressure circulation air disinfection system and its supporting first intelligent control cabinet, negative pressure sterile disinfection box, intelligent pruning inoculation box, and double-layer automatic disinfection door connecting the external seedling bank to be disinfected, replenishment consumable material bank, constant temperature and humidity induction culture room, and constant temperature and humidity rooting culture room.

[0027] The negative pressure sterile disinfection box is equipped with a 0.5 atmosphere negative pressure vacuum disinfection maintenance system, a single-arm intelligent operating robot with visual recognition / sewage and waste suction head / pump-pressure spray cleaning device nozzle, a 70% alcohol immersion disinfection tank, a sterile distilled water tank and pump-pressure spray cleaning device, a 2% NaClO solution tank, a Tween-80 bottle and a peristaltic drip pump system, a double-layer isolation door, and a second intelligent control cabinet connected to each mechanism; the intelligent trimming and inoculation box is equipped with an internal temperature and humidity and negative pressure circulation air disinfection system, a single-arm intelligent flexible forceps robot with visual recognition and positioning, an intelligent parallel surgical robot with visual recognition, a double-layer isolation door, and a third intelligent control cabinet connected to each mechanism.

[0028] Specifically, both the constant temperature and humidity induction culture room and the rooting culture room are equipped with multi-layer intelligent monitoring three-dimensional culture racks, constant temperature and humidity intelligent control systems and their control cabinets, supporting inspection robots and edge computing systems; each layer of the multi-layer intelligent monitoring three-dimensional culture racks holds breeding trays containing breeding cups; the edge computing system controls the multi-layer intelligent monitoring three-dimensional culture racks, constant temperature and humidity intelligent control systems and their control cabinets, and inspection robots via 5G and MES, and controls the culture room temperature to 25±2℃, the light intensity to 2500±500 lux, and the light cycle to 16 hours of light and 8 hours of darkness;

[0029] The humid and warm nursery is equipped with a sensor network, intelligent actuators, an intelligent water and fertilizer integration system, inspection robots, and an edge computing platform. The sensor network is used to monitor the temperature and humidity of the substrate and the pH / EC value of the soil in real time. The intelligent actuators are used to control the skylights, fans, wet curtains, insulation curtains, supplemental lighting, sprinkler and drip irrigation, and fertilizer equipment. The inspection robots are equipped with multispectral and hyperspectral cameras, lidar, air temperature and humidity sensors, light intensity sensors, CO2 concentration sensors, automatic charging and battery swapping stations, laser weeding devices, insecticide spraying devices, a 5G cloud communication system, a robot intelligent navigation and control system, a Beidou positioning and navigation module, and an electronic map of the nursery.

[0030] The sunroom is equipped with a movable intelligent shading film cover, an intelligent movable self-regulating water and fertilizer spraying cart, shading curtains, and an intelligent ventilation control system.

[0031] Specifically, the tertiary biological source bank includes:

[0032] A first-class cryogenic -196°C liquid nitrogen preservation seed bank is equipped with cryogenic insulated liquid nitrogen storage tanks and their maintenance and monitoring systems;

[0033] A secondary low-temperature 4°C tissue culture backup library, equipped with a multi-power supply cooling system and a circulating sterilization system;

[0034] The three-level ambient temperature propagation chamber is equipped with a constant temperature and humidity system and a circulating disinfection system.

[0035] Specifically, in the material storage and transfer unit, the external seed source bank includes an external intelligent seed cache bank, an intelligent disinfection and inoculation consumables and accessories automated storage and retrieval system; the intelligent AGV cart is an intelligent AGV cart with robotic arm and lifting function, used for stacking, recycling, loading, unloading, transferring, adding and cleaning of seedlings, breeding cups, breeding trays and consumables between units;

[0036] The cleaning, disinfection, and culture medium workshop is equipped with AGV trolleys with loading and unloading robotic arms, a fully automatic cup and plate cleaning and disinfection line, a culture medium filling line, culture medium storage tanks and a dispensing and metering pumping system, as well as a warehouse for vulnerable parts and consumables.

[0037] Specifically, the tissue culture seedling transplanting room is equipped with a root seedling transplanting robot, a transplanting preparation line with nutrient soil storage and quantitative addition functions, seedling trays, nursery transfer and unloading robots, and a space circulation disinfection system. The seedling trays are made of biodegradable biomass fiber and slow-release nutrient material after final transplanting. The surface is equipped with a micro QR code that corresponds one-to-one with the QR code of the breeding tray at the breeding stage and the timeline. This QR code can be recognized by relevant robots, intelligent planting and transplanting machines, and edge computing in five stages: greenhouse seedling cultivation, sunshade seedling cultivation and hardening, transportation, on-site hardening, and on-site planting.

[0038] The intelligent transplanter has intelligent speed regulation and steering self-walking function, Beidou positioning and navigation function, electronic map positioning and navigation function for planting plots, automatic hole digging function, root establishment water and fertilizer application function, soil covering and film covering function, and is equipped with a robotic arm for stacking seedling hole cups and trays, cutting seedlings, removing hole cups, and placing hole cups.

[0039] Among the intelligent field equipment: the robot dog is equipped with a working manipulator, a visual recognition system, a laser weeding mechanism, a leaf-picking mechanism, a bird-driving voice control device, and a Beidou navigation module. It is a four-legged heavy-duty working robot used for leaf picking and weeding, tunnel network deployment and retrieval, planting and breeding patrols, security and grazing operations, and regular random sampling; the sampling and transportation drone is used to transport the samples collected by the robot dog to the molecular biology laboratory; the soil sensors include fixed soil sensors that are systematically installed together with intelligent weather stations and wind power and photovoltaic intelligent charging and swapping stations, as well as mobile soil sensors that are carried and charged by the robot dog for fixed-point and regular field testing.

[0040] Specifically, the multiple edge cloud brains deployed in the edge computing and decision-making layer include:

[0041] Edge cloud brains deployed in wind power and photovoltaic smart charging and swapping stations, edge cloud brains deployed in molecular biology laboratories, edge cloud brains deployed in tertiary biological seed banks, edge cloud brains deployed in cleaning, disinfection and culture medium workshops, edge cloud brains deployed in external seed and consumable material banks, edge cloud brains deployed in smart disinfection and inoculation rooms, edge cloud brains deployed in constant temperature and humidity induction culture rooms and constant temperature and humidity rooting culture rooms, edge cloud brains deployed in tissue culture seedling transplanting rooms, edge cloud brains deployed in greenhouse nurseries, edge cloud brains deployed in sun sheds, and edge cloud brains deployed in various processing bases;

[0042] Each edge cloud brain integrates an industrial gateway, a supercomputing RTK base station, an edge node server, a blockchain node database, and various communication modules for loading lightweight AI models. Slow data is transmitted between the edge cloud brain and the data acquisition and execution layer devices using NB-IoT / 4G collaborative communication.

[0043] Specifically, the supercomputing data center for breeding, seedling cultivation, and planting includes: data analysis AI software, a knowledge graph prediction model library, a smart agriculture management system backend, a breeding storage database, a seedling database, and a planting tracking cloud database; used to optimize and release selection, breeding, and seedling cultivation models, growth and biomass composition prediction models, disaster and pest models, carbon sequestration models, and digital twin models; through the hash values, metadata, ownership certificates, and transaction logs of key data, it links with the data centers of various traditional Chinese medicine planting and breeding alliance chains, various energy biomass alliance chains, and various raw material biomass alliance chains to store traceable and immutable data assets; and through germplasm resource registration contracts, breeding data storage contracts, planting process record contracts, quality traceability query contracts, and data authorization access contracts, it links with various edge cloud brains, and provides full-process data and decision support for the apps or interfaces of user-level scientific research cooperation service providers, breeding and seedling manufacturers, planting and plant protection field management service providers, reed asset owners, inter-row planting and breeding contractors, harvesting and transportation service providers, primary processing service providers, deep processing enterprises, end-user customers, government management departments, investment institutions, and financial institutions.

[0044] A fully automated method for the asexual tissue culture propagation of Reed japonica plants, based on a fully automated system for the asexual tissue culture propagation of Reed japonica plants, includes the following steps:

[0045] S1. Seed collection and planting: Collect superior Reed germplasm resources from different ecological zones and plant them in experimental fields in different climate zones. Each single plant forms an asexual line.

[0046] S2. Intelligent data collection for seed selection: Using an intelligent drone platform, every 3 hours from 6 am onwards during the two growth periods, the drone automatically collects phenotypic data of the target nursery or field Reed plants over a large area and at high throughput. The data is then transmitted via 5G and the cloud to the target nursery or field via high throughput. The data includes height, diameter, crown width, biomass, chlorophyll content, nitrogen status, carbon dioxide and oxygen concentration. The data is then sent to the breeding, seedling cultivation and planting supercomputing data center.

[0047] S3. Gene Analysis and Target Seed Source Screening: The supercomputing data center for breeding, seedling cultivation, and planting analyzes phenotypic data, integrates environmental data, and uses artificial intelligence and machine learning algorithms for data analysis to establish predictive models and accurately screen the best and superior clone target seed sources; in the molecular biology laboratory, new shoots of the target seed sources are collected, and DNA molecular marker maps are established through automated nucleic acid extraction devices and high-throughput gene sequencing devices to perform molecular marker-assisted selection and molecular detection marker monitoring;

[0048] S4. Seedling disinfection treatment: The selected target seedlings are sent to an external intelligent seedling buffer, and then transported by an intelligent AGV to the negative pressure sterile disinfection box in the intelligent disinfection and inoculation room; in the negative pressure sterile disinfection box, a fully automated programmed disinfection is performed by a single-arm intelligent robotic arm: 70% alcohol surface immersion disinfection for 30 seconds → sterile distilled water rinsing twice → 2% NaClO solution with 1 drop of Tween-80 for main disinfection for 10 minutes → sterile distilled water rinsing 5 times for 1 minute each time → transfer by robotic arm;

[0049] S5. Pruning, Inoculation, and Propagation: After disinfection, the seedlings are transferred to the intelligent pruning and inoculation box by an intelligent AGV cart. Using a visually-enabled intelligent parallel surgical robot, sterile forceps and a scalpel are used to remove the outer bracts and cut 1mm bud tips or stem tips or axillary buds with a small amount of tissue. These are then inoculated into MS induction medium containing cytokinins in cleaned and disinfected breeding cups to induce the formation of clustered buds. The clustered buds are then monitored by molecular detection and labeling. The intelligent AGV cart then places the breeding tray containing the breeding cups onto a multi-layer intelligent monitoring three-dimensional culture rack in the constant temperature and humidity induction culture room, where clustered buds are cultivated under the control of the corresponding edge cloud brain.

[0050] S6. Rooting and Cultivation: Healthy bud clusters are transferred to an intelligent pruning and inoculation box. A smart parallel surgical robot with visual recognition identifies the healthy bud clusters and inoculates them onto clean, sterilized breeding cups containing auxin-containing MS rooting medium to induce the formation of a complete root system. The inoculated breeding cups are then transferred to a multi-layered intelligent monitoring three-dimensional culture rack in a constant temperature and humidity rooting culture chamber. Under the control of the corresponding edge cloud brain, seedlings are cultivated. Control conditions include: temperature 25±2℃, light intensity 2500±500 lux, and a photoperiod of 16 hours of light and 8 hours of darkness.

[0051] S7. Hardening off and Transplanting in Seedling Trays: The breeding trays for root cultivation are moved into the tissue culture seedling transplanting room, and the breeding cups are opened for hardening off for 3 days. The root seedlings are removed from the breeding cups by the root seedling transplanting robot, and the culture medium is washed with sterile distilled water and then separated into individual plants. The individual root seedlings are placed one by one into the seedling trays on the transplanting preparation line and nutrient soil is added. The surface of the seedling trays is marked with a QR code associated with the QR code information of the breeding cups. The seedling trays are then transported to the greenhouse nursery by the nursery transfer loading and unloading robot.

[0052] S8. Intermediate Processing: Recycling and processing seedling cups, breeding trays, induction medium, rooting medium, and preserving germplasm; using a fully automated cup and tray cleaning and disinfection line to clean and disinfect breeding cups and trays; using a culture medium dispensing line to automatically prepare and dispense culture medium, adding induction medium or rooting medium to the breeding cups in the trays; using a primary ultra-low temperature (-196°C) liquid nitrogen preservation germplasm bank to preserve the most robust clustered shoots, a secondary low temperature (4°C) tissue culture backup bank to rotate and back up the preservation of robust propagation clustered shoots annually, and a tertiary room temperature propagation working bank to preserve or cache germplasm for daily research and propagation; samples preserved in the tertiary biological germplasm bank are monitored by molecular detection and labeling.

[0053] S9. Greenhouse Seedling Acclimation: In a greenhouse nursery, seedlings are placed for 10 days in an environment with a temperature of 23±5℃, humidity of 90±5% and shading rate of 70% for the first 4 days, humidity of 75±5% and shading rate of 50% for the next 6 days, and mist spraying without water accumulation. During this period, environmental parameters are monitored in real time through a sensor network, environmental adjustment equipment is controlled by intelligent actuators, inspection robots perform uninterrupted inspections, and local decision-making and control are performed by the corresponding edge cloud brain.

[0054] S10. Cultivating and Hardening Off Seedlings in Sunrooms: After the seedlings have recovered from transplant shock, transfer the seedling trays and pots to a sunroom. Cultivate strong seedlings for 4 weeks and harden them off for 1 week in an environment with a temperature not lower than 15℃. During the strong seedling stage, the shading rate is 50% when the seedlings are first moved in, and then reduced by 10% each week. Use ventilation to control the day-night temperature difference to 10℃ and control the humidity to 60%. Ventilate for 2 hours each at noon and midnight every day, and spray diluted liquid fertilizer once a week. During the hardening off stage, provide full sun exposure in the open air without water or fertilizer and spray with mist for 1 week.

[0055] S11. On-site hardening-off and transplanting: The seedling trays containing the seedlings to be transplanted are laid flat under a transparent rain shelter at the planting site for one week of on-site hardening-off. On the deeply tilled and ridged planting plots, intelligent planting and transplanting machines are used for transplanting. The intelligent planting and transplanting machines automatically locate and navigate based on Beidou positioning and navigation and the electronic map of the planting plot, and perform operations such as digging holes, removing seedling trays, placing seedling trays, applying water and fertilizer for root establishment, covering with soil, and covering with film. After transplanting, the seedlings will recover for 2 weeks until new buds emerge, and the seedlings will have survived.

[0056] S12. Field Management and Data Collection: After transplanting, a smart drone platform is used to regularly collect phenotypic data of reeds; climate data is collected through an on-site smart weather station, and soil moisture, EC value, pH value, and NPK data are collected through an on-site soil sensor network; robotic dogs are used for weeding and leaf removal between rows, tunnel network deployment and retrieval, planting and breeding patrols, and security and grazing operations, and random samples are taken from the leaves picked between rows at regular intervals; the samples collected by the robotic dogs are transported by sampling and transport drones to the molecular biology laboratory for gene sequencing to obtain gene tracking and traceability data; the charging and swapping operations of the above-mentioned drones and robotic dogs, as well as the power supply of the smart weather station and soil sensors, are all provided by the wind power and photovoltaic smart charging and swapping station;

[0057] S13. Data Upload and Edge Decision-Making: Data collected at each stage is transmitted to the edge cloud brain of the respective domain via NB-IoT / 4G / 5G; the edge cloud brain processes the real-time data, executes local decisions, controls the facilities, equipment, robots, smart agricultural machinery and sensor networks of the respective domain, and issues operation instructions, operation paths and prescription maps; the edge cloud brain uploads key events, aggregated data and summaries to the breeding, seedling and planting supercomputing data center via satellite network / 5G / 4G / WIFI, and receives the issued optimization strategies and AI model updates;

[0058] S14. Harvesting and Data Traceability: After the reeds mature, they are harvested and packaged by intelligent harvesters, and the harvesting data and package marking data are uploaded to the edge cloud brain of the wind power and photovoltaic intelligent charging and swapping station. The edge cloud brain combines the harvesting data with the original traceability data to form a traceability data chain, which is then uploaded to the breeding, seedling cultivation and planting supercomputing data center. The supercomputing data center, through data cleaning, sorting, aggregation and blockchain encryption links, links the hash values, metadata, ownership certificates and transaction logs of key data with the data centers of various Chinese herbal medicine planting and breeding alliance chains, various energy biomass alliance chains, and various raw material biomass alliance chains, storing them to form traceable and immutable data assets.

[0059] S15. Data Application and Decision Support: Through germplasm resource registration contracts, breeding data storage contracts, planting process record contracts, quality traceability query contracts, and data authorization access contracts, and by linking with the apps or interfaces of user-level scientific research cooperation service providers, breeding and seedling suppliers, planting, plant protection and field management service providers, reed asset owners, harvesting and transportation service providers, processing service providers, end customers, government management departments, and investment and financial institutions, we provide full-process data and decision support.

[0060] The beneficial effects of this invention are:

[0061] Intelligent coverage throughout the entire process: This invention is the first to construct an intelligent system for the entire life cycle of Phragmites australis, from seed selection and breeding to plant protection and harvesting, realizing a closed loop of "perception-decision-execution" and filling the technological gap of intelligent full-chain management of Phragmites australis plants.

[0062] Significantly shorten the breeding cycle: By using high-throughput phenotypic data collection by drones, molecular marker-assisted selection, and AI data analysis, superior germplasm sources can be accurately screened, shortening the traditional breeding cycle by more than 30%.

[0063] Improving seedling survival rate and quality: Intelligent environmental control and robotic operation reduce human pollution, resulting in a significant improvement in seedling uniformity and survival rate.

[0064] Traceable data assets: A full lifecycle database is built based on blockchain technology to ensure that data from seed source, cultivation, planting, and harvesting are tamper-proof, supporting quality traceability and multi-party collaboration, and forming tradable data assets.

[0065] Reduce labor costs: Robots replace human labor in tasks such as disinfection, inoculation, transplanting, inspection, and sampling, reducing reliance on human labor and labor intensity, and solving the bottleneck of manpower-intensive operations.

[0066] Clean energy self-sufficiency: Wind and solar power smart charging and swapping stations provide clean energy for field equipment, adapt to deployment in remote areas, and reduce operating costs. Attached Figure Description

[0067] Figure 1 This is a schematic diagram of the system architecture of the present invention;

[0068] Figure 2 This is a schematic diagram of the method flow of the present invention;

[0069] Figure 3 This is a schematic diagram of the communication link of the intelligent facility and equipment for Reed breeding according to the present invention;

[0070] Figure 4 This is a schematic diagram of the communication link of the intelligent facility and equipment for Phragmites australis seedling cultivation according to the present invention;

[0071] Figure 5 This is a schematic diagram of the intelligent reed plant protection, inter-row planting and breeding, and harvesting and processing data link of the present invention;

[0072] Figure 6 This is a flowchart of the data and control system of the intelligent system for the entire process of reed cultivation according to the present invention;

[0073] The following will describe in detail, with reference to the accompanying drawings, embodiments of the present invention. Detailed Implementation

[0074] The present invention will be further described below with reference to embodiments:

[0075] like Figures 1-6 As shown, an intelligent system for the entire process of asexual tissue culture propagation of Reed ferns includes a data acquisition and execution layer, an edge computing and decision-making layer, and a cloud data processing and storage layer.

[0076] The intelligent system of this invention includes the following: intelligent drone phenotypic screening for seed source selection, molecular biology laboratory gene sequencing for seed source selection, intelligent disinfection and cleaning of seedlings, storage of superior varieties in a three-level seed bank, fully intelligent breeding, fully intelligent seedling raising, robot phenotypic collection and identification, intelligent breeding and propagation of superior varieties, intelligent transplanter for optimized planting, intelligent drone phenotypic collection and quality assurance field management, channel robot dog for leaf picking and ventilation, carbon sink monitoring and gene sampling, and intelligent crushing or cutting harvester for harvesting and packaging.

[0077] This invention integrates IoT, AI, big data, and robotics technologies for asexually propagated plants like Reed, covering the entire process from seed selection and breeding to quality-assured harvesting, and constructs a closed loop of "perception-decision-execution".

[0078] The data acquisition and execution layer includes:

[0079] The intelligent drone platform is used to collect phenotypic data of Arundinaria mirifica plants and perform plant protection and field management operations. Specifically, the intelligent drone platform is equipped with multispectral / hyperspectral lidar, air composition detection sensors, Beidou precise positioning system, geographic information electronic map, high-definition camera and identification system with infrared night vision function, and satellite 5G communication system. It is used to automatically collect phenotypic data of Arundinaria mirifica plants on a large area and with high throughput, including height, diameter, crown width, biomass, chlorophyll content, nitrogen status, carbon dioxide and oxygen concentration.

[0080] The molecular biology laboratory is equipped with automated nucleic acid extraction devices, high-throughput gene sequencing devices, and bioinformatics analysis software systems. These systems are used to obtain genotype data of plants, establish DNA molecular marker maps, and realize molecular marker-assisted selection and molecular detection marker monitoring, that is, to mark the source of germplasm by region, block, location, time, environment, and batch.

[0081] The intelligent disinfection and inoculation room is used for automated disinfection and pruning inoculation of seedlings. Specifically, the intelligent disinfection and inoculation room is equipped with an indoor temperature and humidity and negative pressure circulation air disinfection system and its supporting first intelligent control cabinet, negative pressure sterile disinfection box, intelligent pruning and inoculation box, and double-layer automatic disinfection door connecting to the external seedling bank to be disinfected, replenishment consumable material bank, constant temperature and humidity induction culture room, and constant temperature and humidity rooting culture room.

[0082] The negative pressure sterile disinfection box is equipped with a 0.5 atmosphere negative pressure vacuum disinfection maintenance system, a single-arm intelligent operating robot with visual recognition / sewage and waste suction head / pump-pressure spray cleaning device nozzle, a 70% alcohol immersion disinfection tank, a sterile distilled water tank and pump-pressure spray cleaning device, a 2% NaClO solution tank, a Tween-80 bottle and a peristaltic drip pump system, a double-layer isolation door, and a second intelligent control cabinet connected to each mechanism. The fully automatic disinfection process is as follows: fully automatic program operation of 70% alcohol surface immersion disinfection for 30 seconds → sterile distilled water rinsing twice → 2% NaClO (sodium hypochlorite) solution with 1 drop of Tween-80 for main disinfection for 10 minutes → sterile distilled water rinsing 5 times for 1 minute each time → robot transfer.

[0083] The intelligent pruning and inoculation box is equipped with an internal temperature and humidity control system with negative pressure circulation for air disinfection, a single-arm intelligent flexible forceps robotic arm with visual recognition and positioning, an intelligent parallel surgical robotic arm with visual recognition, a double-layer isolation door, and a third intelligent control cabinet connecting to various mechanisms. The specific process for pruning, inoculation, and propagation within the intelligent pruning and inoculation box is as follows: Moisture is absorbed using sterile filter paper; the outer bracts are removed using sterile forceps and a scalpel by the intelligent parallel surgical robotic arm (surgical robot) with visual recognition; bud tips or stem tips (1mm) or axillary buds with a small amount of tissue are cut and inoculated into clean, sterilized glass breeding cups on MS medium containing cytokinin to induce the formation of clustered buds (e.g., with the addition of appropriate antibiotics or antioxidants).

[0084] The material storage and transfer unit includes an external seed bank and intelligent AGV carts, used for storing materials and transferring them between units. Specifically, the external seed bank includes an external intelligent seed cache, an intelligent disinfection and inoculation consumables and accessories automated storage and retrieval system. The intelligent AGV carts are equipped with robotic arms and lifting functions, used for stacking, recycling, loading, unloading, transferring, adding, and cleaning seedlings, breeding cups, breeding trays, and consumables between units (for the negative pressure sterile box and the intelligent pruning and inoculation box, as well as between the intelligent disinfection and inoculation room and the constant temperature and humidity induction culture room, the constant temperature and humidity rooting culture room, the three-level seed bank, the handover window, and the supplementary consumables storage window).

[0085] The seedling cultivation unit includes a constant temperature and humidity induction culture room, a constant temperature and humidity rooting culture room, a greenhouse nursery, and a sunroom, used for intelligent seedling cultivation. Specifically, the constant temperature and humidity induction culture room and the rooting culture room are equipped with multi-layer intelligent monitoring three-dimensional cultivation racks, a constant temperature and humidity intelligent control system and its control cabinet, a matching inspection robot, and an edge computing system. Each layer of the multi-layer intelligent monitoring three-dimensional cultivation rack holds a cultivation tray containing breeding cups. The matching inspection robot has intelligent walking and intelligent lifting functions, and is equipped with a left and right rotating telescopic robotic arm with a vision recognition system, temperature monitoring function, humidity monitoring function, light monitoring function, a database, a wireless communication module, and a robot control system. The edge computing system controls the multi-layer intelligent monitoring three-dimensional cultivation racks, the constant temperature and humidity intelligent control system and its control cabinet, and the inspection robot through 5G and MES, and controls the temperature of the cultivation room to 25±2℃, the light intensity to 2500±500 lux, and the light cycle to 16 hours of light and 8 hours of darkness.

[0086] Clump shoots are cultivated in a constant temperature and humidity induction culture room. Shoots are then cultivated in breeding trays containing breeding cups, arranged on each layer of a multi-layer intelligent monitoring three-dimensional culture rack. Rooting inoculation is performed in an intelligent pruning inoculation box in a sterilized inoculation room. Healthy clump shoots are identified using sterile forceps and a scalpel by an intelligent parallel surgical robot with visual recognition, and inoculated into clean, sterilized glass breeding cups containing MS rooting medium containing auxin (such as NAA) to induce the formation of a complete root system. Seedlings are cultivated in a constant temperature and humidity rooting culture room, and rooting is performed in breeding trays containing breeding cups, arranged on each layer of a multi-layer intelligent monitoring three-dimensional culture rack.

[0087] The humid and warm nursery is equipped with a sensor network, intelligent actuators, an intelligent fertigation system, inspection robots, and an edge computing system. The sensor network monitors the substrate temperature and humidity, and soil pH / EC values ​​in real time. The intelligent actuators control skylights, fans, wet curtains, insulation curtains, supplemental lighting, sprinkler and drip irrigation, and fertilizer equipment. The inspection robots are equipped with multispectral and hyperspectral cameras, lidar, air temperature and humidity sensors, light intensity sensors, CO2 concentration sensors, automatic charging and battery swapping stations, laser weeding devices, insecticide spraying devices, a 5G cloud communication system, a robot intelligent navigation and control system, a BeiDou positioning and navigation module, and an electronic map of the nursery. The edge computing system controls the sensor network, intelligent actuators, intelligent fertigation system, the inspection robots' production management system (MES), AI image recognition system, a knowledge graph of reed seedling cultivation, and the 5G communication system.

[0088] The sunroom is equipped with a movable intelligent shading film canopy, an intelligent movable self-regulating water and fertilizer spraying cart, shading curtains, and an intelligent ventilation control system. A glass sunroom can also be used.

[0089] A tertiary biological germplasm bank is used for the graded preservation of germplasm resources; specifically, a tertiary biological germplasm bank includes:

[0090] A first-class cryogenic -196°C liquid nitrogen preservation seed bank is equipped with cryogenic insulated liquid nitrogen storage tanks and their maintenance and monitoring systems;

[0091] A secondary low-temperature 4°C tissue culture backup library, equipped with a multi-power supply cooling system and a circulating sterilization system;

[0092] The three-level ambient temperature propagation chamber is equipped with a constant temperature and humidity system and a circulating disinfection system.

[0093] The cleaning, disinfection, and culture medium workshop is used for cleaning and disinfecting culture containers and preparing culture media. Specifically, the cleaning, disinfection, and culture medium workshop is equipped with AGV trolleys equipped with loading and unloading robotic arms, fully automatic cup and plate cleaning and disinfection lines, culture medium filling lines, culture medium storage tanks and dispensing and metering pumping systems, and a warehouse for vulnerable parts and consumables.

[0094] The tissue culture seedling transplanting room is used to harden off tissue culture seedlings and transplant them to the greenhouse nursery. Specifically, the tissue culture seedling transplanting room is equipped with a root seedling transplanting robot, a transplanting preparation line with nutrient soil storage and quantitative addition functions, seedling trays, nursery transfer and unloading robots, and a space circulation disinfection system. The seedling trays are made of biodegradable biomass fiber and slow-release nutrient material after final transplanting. The surface is equipped with a micro QR code that corresponds one-to-one with the QR code of the breeding tray at the breeding stage and the timeline. This QR code can be recognized by relevant robots, intelligent planting and transplanting machines, and edge computing in five stages: greenhouse seedling cultivation, sunshade seedling hardening, transportation, on-site seedling hardening, and on-site planting.

[0095] The intelligent planting and transplanting machine is used to automatically transplant seedlings from seedling trays to the field. Specifically, the intelligent transplanting machine has intelligent speed regulation and steering self-walking function, Beidou positioning and navigation function, electronic map positioning and navigation function for planting plots, automatic hole digging function, root establishment water and fertilizer application function, soil covering and film covering function, and is equipped with a robotic arm for stacking seedling trays, cutting seedlings, removing seedling trays, and placing seedling trays.

[0096] The field intelligent equipment includes intelligent weather stations, soil sensors, water and fertilizer irrigation systems, robotic dogs for inter-row operations and sampling, and sampling and transport drones. Specifically, the field intelligent equipment includes: robotic dogs equipped with a working manipulator, a visual recognition system, a laser weeding mechanism, a leaf-picking mechanism, a bird-driving voice control device, and a Beidou navigation module. These are quadrupedal heavy-duty robots used for leaf picking and weeding, tunnel network deployment and retrieval, planting and breeding patrols, security and grazing operations, and regular random sampling; sampling and transport drones are used to transport samples collected by the robotic dogs to molecular biology laboratories; soil sensors include fixed soil sensors that are systematically installed together with intelligent weather stations and wind power and photovoltaic intelligent charging and swapping stations, as well as mobile soil sensors carried and charged by the robotic dogs for fixed-point and regular field monitoring.

[0097] The intelligent harvester is used for the automated harvesting of mature reeds. The intelligent harvester is a reed crushing or cutting type intelligent harvester. Its harvesting, baling, and bag marking data are continuously uploaded to the edge cloud brain of the wind power and photovoltaic intelligent charging and swapping station. Together with the original traceability data, it forms a traceability data chain and enters the local breeding, seedling and planting supercomputing data center.

[0098] The wind and solar smart charging and swapping station integrates an RTK base station and an edge cloud brain to provide charging and swapping services and positioning references for on-site equipment. The smart planting and transplanting machine, smart drone platform, and supporting drones, robotic dogs, and smart harvesters are all precisely positioned and navigated using the RTK base station of the wind and solar smart charging and swapping station in collaboration with the electronic map of the planting area in the edge cloud brain of the smart charging and swapping station, and communicate in real time via 5G. The electronic map of the planting area in the edge cloud brain of the smart charging and swapping station is also created through remote sensing and continuous precision calibration by the smart planting and transplanting machine, smart drone platform, and supporting drones, robotic dogs, and smart harvesters.

[0099] The edge computing and decision-making layer includes multiple edge cloud brains deployed in various key functional units. Each edge cloud brain receives and processes real-time data from the data acquisition and execution layer, executes local decisions, and controls devices in its assigned area. Specifically, the multiple edge cloud brains deployed in the edge computing and decision-making layer include:

[0100] Edge cloud brains deployed in wind power and photovoltaic smart charging and swapping stations, edge cloud brains deployed in molecular biology laboratories, edge cloud brains deployed in tertiary biological seed banks, edge cloud brains deployed in cleaning, disinfection and culture medium workshops, edge cloud brains deployed in external seed and consumable material banks, edge cloud brains deployed in smart disinfection and inoculation rooms, edge cloud brains deployed in constant temperature and humidity induction culture rooms and constant temperature and humidity rooting culture rooms, edge cloud brains deployed in tissue culture seedling transplanting rooms, edge cloud brains deployed in greenhouse nurseries, edge cloud brains deployed in sun sheds, and edge cloud brains deployed in various processing bases;

[0101] Each edge cloud brain integrates an industrial gateway, a supercomputing RTK base station, an edge node server, a blockchain node database, and various communication modules for loading lightweight AI models. Slow data is transmitted between the edge cloud brain and devices in the data acquisition and execution layer via NB-IoT / 4G collaboration. Specifically, the edge cloud brain can selectively integrate various hardware and communication methods, such as industrial gateways, supercomputing RTK base stations, edge node servers, and blockchain node databases, depending on the needs of the linked objects and control scenarios. Communication supports 4G / 5G / WiFi, BeiDou / GPS satellite internet, and NB-IoT. The system embeds a lightweight AI model, supports integration with Huawei or Alibaba Cloud IoT platforms, and can deploy multiple application nodes at the edge, including farm management platforms, primary / refined processing MES, supply chain management systems, agricultural product traceability SaaS platforms, and smart agriculture management system middleware, enabling intelligent collaboration in agricultural production and management.

[0102] The cloud-based data processing and storage layer includes a supercomputing data center for breeding, seedling cultivation, and planting. This supercomputing data center communicates with various edge cloud brains to aggregate multi-source data, run AI algorithm models and knowledge graph prediction model libraries, and, through blockchain technology, form a distributed encrypted storage with various Chinese herbal medicine planting and breeding alliance chain data centers, various energy biomass alliance chain data centers, and various raw material biomass alliance chain data centers to build tamper-proof, full-lifecycle data assets.

[0103] Specifically, the supercomputing data center for breeding, seedling cultivation, and planting includes: data analysis AI software, a knowledge graph prediction model library, a smart agriculture management system backend, a breeding storage database, a seedling database, and a planting tracking cloud database; used to optimize and release selection, breeding, and seedling cultivation models, growth and biomass composition prediction models, disaster and pest models, carbon sequestration models, and digital twin models; through the hash values, metadata, ownership certificates, and transaction logs of key data, it links with the data centers of various traditional Chinese medicine planting and breeding alliance chains, various energy biomass alliance chains, and various raw material biomass alliance chains to store traceable and immutable data assets; and through germplasm resource registration contracts, breeding data storage contracts, planting process record contracts, quality traceability query contracts, and data authorization access contracts, it links with various edge cloud brains, and provides full-process data and decision support for the apps or interfaces of user-level scientific research cooperation service providers, breeding and seedling manufacturers, planting and plant protection field management service providers, reed asset owners, inter-row planting and breeding contractors, harvesting and transportation service providers, primary processing service providers, deep processing enterprises, end-user customers, government management departments, investment institutions, and financial institutions.

[0104] A fully automated method for the asexual tissue culture propagation of Reed japonica plants, based on a fully automated system for the asexual tissue culture propagation of Reed japonica plants, includes the following steps:

[0105] S1. Seed collection and planting: Collect superior Reed germplasm resources from different ecological zones and plant them in experimental fields in different climate zones, with each individual plant forming an asexual line.

[0106] S2. Intelligent Data Collection for Seed Selection: Using an intelligent drone platform, the accompanying intelligent drones automatically collect phenotypic data of Phyllostachys edulis plants in the target nursery or field every 3 hours from 6 am every day during the two growth periods. The data is collected over a large area and transmitted to the cloud via 5G and cloud. The data includes height, diameter, crown width, biomass, chlorophyll content, nitrogen status, carbon dioxide and oxygen concentration. The data is then sent to the breeding, seedling cultivation and planting supercomputing data center.

[0107] S3. Gene Analysis and Target Progeny Screening: The breeding, seedling cultivation, and planting supercomputing data center (equipped with superior variety data analysis and marker storage functions, analyzes the phenotypic and environmental parameters of Arundo donax types such as robust and tall plants, the largest number of branches, fastest growth, cold, drought, heat, salt and alkali resistance, disease and pest resistance, highest cellulose content, highest carbon content, and non-flowering and seed-bearing, and integrates genotype and pedigree data to construct a breeding database) to analyze phenotypic data, integrate environmental data, and use artificial intelligence and machine learning algorithms for data analysis to establish predictive models and accurately screen the best and superior clonal target progeny; in the molecular biology laboratory, new shoots of the target progeny are collected, and DNA molecular marker maps are established through automated nucleic acid extraction devices and high-throughput gene sequencing devices for molecular marker-assisted selection and molecular detection marker monitoring.

[0108] In the molecular biology laboratory C, molecular detection and marker monitoring are implemented. Seedlings are collected and DNA molecular maps are established using an intelligent genotyping platform. Specifically, the terminal buds, shoot tips, and axillary buds of the target seed source are collected to establish DNA molecular marker maps. DNA molecular marker-assisted selection (MAS) enables rapid propagation and virus-free tissue culture of the target seed source. The terminal buds and axillary buds (shoot tips) of the new seedlings from superior seed sources in spring, summer, and autumn are collected to rapidly propagate superior clones, eliminate viral diseases, restore seed characteristics, and develop DNA molecular marker maps linked to superior traits (such as disease resistance and high cellulose). Early and precise screening of offspring can be carried out at the seedling stage, which greatly shortens the breeding cycle.

[0109] S4. Seedling disinfection treatment: The selected target seedlings are sent to an external intelligent seedling buffer, and then transported by an intelligent AGV to the negative pressure sterile disinfection box in the intelligent disinfection and inoculation room. In the negative pressure sterile disinfection box, a fully automated programmed disinfection is performed by a single-arm intelligent robotic arm: 70% alcohol surface immersion disinfection for 30 seconds → sterile distilled water rinsing twice → 2% NaClO solution with 1 drop of Tween-80 for main disinfection for 10 minutes → sterile distilled water rinsing five times for 1 minute each time → transfer by robotic arm.

[0110] S5. Pruning, Inoculation, and Propagation: After disinfection, the seedlings are transferred to the intelligent pruning and inoculation box by an intelligent AGV cart. Using a visually-enabled intelligent parallel surgical robot, sterile forceps and a scalpel are used to remove the outer bracts and cut 1mm bud tips or stem tips or axillary buds with a small amount of tissue. These are then inoculated into MS medium containing cytokinins in cleaned and disinfected breeding cups to induce the formation of clustered buds. The clustered buds are then monitored by molecular detection and labeling. The intelligent AGV cart then places the breeding trays containing the breeding cups onto a multi-layer intelligent monitoring three-dimensional culture rack in the constant temperature and humidity induction culture room, where clustered buds are cultivated under the control of the corresponding edge cloud brain.

[0111] S6. Rooting and Cultivation: Healthy bud clusters are transferred to an intelligent pruning and inoculation box. The healthy bud clusters are identified by an intelligent parallel surgical robot with visual recognition and inoculated into rooting MS medium containing auxin in clean and sterilized breeding cups to induce the formation of a complete root system. The inoculated breeding cups are then transferred to a multi-layer intelligent monitoring three-dimensional culture rack in a constant temperature and humidity rooting culture room. Under the control of the corresponding edge cloud brain, seedlings are cultivated. The control conditions include: temperature of 25±2℃, light intensity of 2500±500 lux, and a photoperiod of 16h light and 8h darkness.

[0112] S7. Hardening off and Transplanting in Seedling Trays: The breeding trays for root cultivation are moved into the tissue culture seedling transplanting room, and the breeding cups are opened for hardening off for 3 days. The root seedlings are removed from the breeding cups by a root seedling transplanting robot, and the culture medium is washed with sterile distilled water mist spray and then separated into individual plants. The individual root seedlings are placed one by one into the seedling trays on the transplanting preparation line and nutrient soil is added. The surface of the seedling trays is marked with a QR code associated with the QR code information of the breeding cup. The seedling trays are transferred to the greenhouse nursery by a nursery transfer loading and unloading robot. The space circulation disinfection system continuously disinfects the tissue culture seedling transplanting room in shifts.

[0113] S8. Intermediate Material Processing: Recycling and processing seedling cups, breeding trays, induction medium, rooting medium, and preserving germplasm; using a fully automated cup and tray cleaning and disinfection line to clean and disinfect breeding cups and trays; using a culture medium dispensing line to automatically prepare and dispense culture medium, adding induction medium or rooting medium to the breeding cups in the trays; using a primary ultra-low temperature (-196°C) liquid nitrogen preservation germplasm bank to preserve the most robust clustered shoots, a secondary low temperature (4°C) tissue culture backup bank to rotate and back up the robust propagation clustered shoots annually, and a tertiary room temperature propagation working bank to preserve or cache germplasm for daily research and propagation; the samples preserved in the tertiary biological germplasm bank are monitored by molecular detection and labeling.

[0114] S9. Greenhouse Seedling Acclimation: In a greenhouse nursery, seedlings are placed for 10 days in an environment with a temperature of 23±5℃, humidity of 90±5% and shading rate of 70% for the first 4 days, humidity of 75±5% and shading rate of 50% for the next 6 days, and mist spraying without water accumulation. The nursery's transport, loading, unloading, and stacking are handled by robots. During this period, environmental parameters are monitored in real time through a sensor network, and environmental control equipment is linked and controlled by intelligent actuators. Inspection robots perform continuous inspections, and local decision-making and control are achieved through corresponding edge computing systems. Specifically, a sensor network is deployed within the seedling greenhouse to monitor parameters such as substrate temperature and humidity, and soil pH / EC values ​​in real time. Intelligent actuators are linked and control skylights and fans. The system includes equipment such as wet curtains, heat-insulating curtains, supplemental lighting, sprinkler and drip irrigation, and fertilizer machines, which intelligently control and adjust the environment to its optimal state. The intelligent water and fertilizer integration system pre-sets irrigation and fertilization programs according to the fertilizer and water requirements of reeds at different growth stages, automatically and precisely mixes and delivers water and fertilizer solutions in proportion, and evenly supplies each seedling through the drip irrigation system. The system also includes inspection robots equipped with multispectral and hyperspectral cameras, lidar, air temperature and humidity sensors, light intensity sensors, CO2 concentration sensors, automatic charging and battery swapping nests, laser weeding devices, insecticide spraying devices, 5G cloud communication systems, robot intelligent navigation and control systems, Beidou positioning and navigation modules, and nursery electronic maps for continuous inspection operations.

[0115] S10. Seedling Cultivation and Hardening-off in Sunlit Greenhouse: After the seedlings have recovered from transplant shock, the seedling trays and pots are transferred to the sunlit greenhouse. Strong seedlings are cultivated for 4 weeks and hardened off for 1 week in an environment with a temperature not lower than 15℃. During the strong seedling stage, the initial shading rate is 50%, decreasing by 10% each week thereafter. Ventilation is used to control the day-night temperature difference to 10℃ and humidity to 60%. Ventilation is provided for 2 hours each at noon and midnight daily. A diluted liquid fertilizer is sprayed once a week (for the first two weeks, the EC value is 1 mS / cm, using a balanced NPK fertilizer of 20-20-20 at a concentration of 1500 times; for the next two weeks, the EC value is 2 mS / cm, using a balanced NPK fertilizer of 15-15-30 at a concentration of 1000 times). During the hardening-off stage, the seedlings are exposed to full sun without water or fertilizer and sprayed with a mist for 1 week. The loading, unloading, and stacking are handled by a robot from the nursery.

[0116] S11. On-site hardening-off and transplanting: The seedling trays containing the seedlings to be transplanted are laid flat under a transparent rain shelter at the planting site for one week of on-site hardening-off. On the deeply tilled and ridged planting plot, an intelligent planting and transplanting machine is used for transplanting. The intelligent planting and transplanting machine automatically locates and navigates according to the Beidou positioning navigation and the electronic map of the planting plot, and performs the operations of digging holes, removing seedling trays, placing seedling trays, applying root-setting water and fertilizer, covering with soil, and covering with film. After transplanting, the seedlings are allowed to recover for 2 weeks until new buds grow, and the seedlings are successfully planted and survived.

[0117] S12. Field Management and Data Collection: After transplanting, intelligent drone platforms are used to regularly collect phenotypic data of reeds; climate data (such as temperature, humidity, wind speed, light, and rainfall) are collected through on-site intelligent weather stations; soil moisture, EC value, pH value, and NPK data are collected through on-site soil sensor networks; data are collected through on-site intelligent water and fertilizer integrated drip irrigation systems; and data are collected by robot dogs for tasks such as leaf picking and weeding between rows, tunnel network deployment and retrieval, planting and breeding patrols, and security and grazing operations. Data is also collected by intelligent sheds and harvesting equipment for Chinese medicinal herbs. The above data is sent to the edge cloud brain of the wind power and photovoltaic intelligent charging and swapping station integrated with RTK base stations, and then uploaded to the local breeding and seedling planting supercomputing data center. The robot dog periodically and randomly samples leaves from between rows; the samples collected by the robot dog are transported by a sampling and transport drone to a molecular biology laboratory for gene sequencing to obtain gene tracking and origin data (and the data is uploaded to the local breeding, seedling and planting supercomputing data center through the edge cloud brain of the molecular biology laboratory to become breeding gene tracking and origin data); the charging and swapping operations of the above-mentioned drones and robot dogs, as well as the power supply of the intelligent weather station and soil sensors, are all provided by wind power and photovoltaic intelligent charging and swapping stations; and the rural agricultural power grid provides power as a backup and supplement.

[0118] S13. Data Upload and Edge Decision-Making: Data collected at each stage is transmitted to the edge cloud brain of the respective domain via NB-IoT / 4G / 5G; the edge cloud brain processes the real-time data, executes local decisions, controls the facilities, equipment, robots, smart agricultural machinery and sensor networks of the respective domain, and issues operation instructions, operation paths and prescription maps; the edge cloud brain uploads key events, aggregated data and summaries to the breeding, seedling and planting supercomputing data center via satellite network / 5G / 4G / WIFI, and receives the issued optimization strategies and AI model updates;

[0119] S14. Harvesting and Data Traceability: After the reeds mature, they are harvested and packaged by intelligent harvesters, and the harvesting data and package marking data are uploaded to the edge cloud brain of the wind power and photovoltaic intelligent charging and swapping station. The edge cloud brain combines the harvesting data with the original traceability data to form a traceability data chain, which is then uploaded to the breeding, seedling cultivation and planting supercomputing data center. The supercomputing data center, through data cleaning, sorting, aggregation and blockchain encryption links, links the hash values, metadata, ownership certificates and transaction logs of key data with the data centers of various Chinese herbal medicine planting and breeding alliance chains, various energy biomass alliance chains, and various raw material biomass alliance chains, storing them to form traceable and immutable data assets.

[0120] S15. Data Application and Decision Support: Through germplasm resource registration contracts, breeding data storage contracts, planting process record contracts, quality traceability query contracts, and data authorization access contracts, and by linking with the apps or interfaces of user-level scientific research cooperation service providers, breeding and seedling suppliers, planting, plant protection and field management service providers, reed asset owners, harvesting and transportation service providers, processing service providers, end customers, government management departments, and investment and financial institutions, we provide full-process data and decision support.

[0121] In the description of this invention, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," and "circumferential" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0122] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this invention, "a plurality of" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0123] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, an electrical connection, or a connection that allows communication between them; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0124] The present invention has been described above by way of example. Obviously, the specific implementation of the present invention is not limited to the above-described manner. Any improvements made by adopting the inventive concept and technical solution of the present invention, or direct application to other occasions without modification, are all within the protection scope of the present invention.

Claims

1. A fully intelligent system for the entire process of asexual tissue culture propagation of Reed (Phragmites australis), characterized in that, include: The data acquisition and execution layer includes: Intelligent drone platform for collecting phenotypic data of Reed hyacinth plants and performing plant protection and field management operations; The molecular biology laboratory is equipped with automated nucleic acid extraction devices, high-throughput gene sequencing devices, and bioinformatics analysis software systems to obtain plant genotype data, establish DNA molecular marker maps, realize molecular marker-assisted selection, and mark the source of seed by region, block, location, time, environment, and batch. The intelligent disinfection and inoculation room is used for automated disinfection and pruning inoculation of seedlings; The material storage and transfer unit, including an external seed bank and intelligent AGV carts, is used for storing materials and transferring them between units. The seedling cultivation unit includes a constant temperature and humidity induction culture room, a constant temperature and humidity rooting culture room, a greenhouse nursery, and a sunroom, which are used for intelligent seedling cultivation. A three-tiered biological germplasm bank is used for the graded preservation of germplasm resources. Cleaning and disinfection and culture medium workshop, used for cleaning and disinfecting culture containers and preparing culture media; The tissue culture seedling transplanting room is used to harden off tissue culture seedlings and transplant them to the greenhouse nursery. Intelligent planting and transplanting machine is used to automatically transplant seedlings from seedling trays to the field; Intelligent field equipment includes intelligent weather stations, soil sensors, water and fertilizer irrigation systems, robotic dogs for inter-row operations and sampling, and sampling and transportation drones; Intelligent harvesters are used for the automated harvesting of mature reeds. The smart charging and swapping station for wind and solar power integrates an RTK base station and an edge cloud brain to provide charging and swapping services and positioning references for field equipment; The edge computing and decision-making layer includes multiple edge cloud brains deployed in each key functional unit. Each edge cloud brain is used to receive and process real-time data from the data acquisition and execution layer, execute local decisions, and control devices in its assigned area. The cloud-based data processing and storage layer includes a supercomputing data center for breeding, seedling cultivation, and planting. This supercomputing data center communicates with various edge cloud brains to aggregate multi-source data, run AI algorithm models and knowledge graph prediction model libraries, and, through blockchain technology, form a distributed encrypted storage with various Chinese herbal medicine planting and breeding alliance chain data centers, various energy biomass alliance chain data centers, and various raw material biomass alliance chain data centers to build tamper-proof, full-lifecycle data assets.

2. The intelligent system for the entire process of asexual tissue culture propagation of Reed japonica plants according to claim 1, characterized in that, The intelligent drone platform is equipped with multispectral / hyperspectral lidar, air composition detection sensors, BeiDou precise positioning system, geographic information electronic map, high-definition camera and identification system with infrared night vision function, and satellite 5G communication system. It is used to automatically collect phenotypic data of Reed Plant height, diameter, crown width, biomass, chlorophyll content, nitrogen status, carbon dioxide and oxygen concentration over a large area and with high throughput.

3. The intelligent system for the entire process of asexual tissue culture propagation of Reed japonica plants according to claim 1, characterized in that, The intelligent disinfection and inoculation room is equipped with an indoor temperature and humidity and negative pressure circulation air disinfection system and its supporting first intelligent control cabinet, negative pressure sterile disinfection box, intelligent pruning and inoculation box, as well as double-layer automatic disinfection doors that connect to the external seedling bank to be disinfected, replenishment consumable material bank, constant temperature and humidity induction culture room, and constant temperature and humidity rooting culture room. The negative pressure sterile disinfection box is equipped with a 0.5 atmosphere negative pressure vacuum disinfection maintenance system, a single-arm intelligent operating robot with visual recognition / sewage and waste suction head / pump-pressure spray cleaning device nozzle, a 70% alcohol immersion disinfection tank, a sterile distilled water tank and pump-pressure spray cleaning device, a 2% NaClO solution tank, a Tween-80 bottle and a peristaltic drip pump system, a double-layer isolation door, and a second intelligent control cabinet connected to each mechanism; the intelligent trimming and inoculation box is equipped with an internal temperature and humidity and negative pressure circulation air disinfection system, a single-arm intelligent flexible forceps robot with visual recognition and positioning, an intelligent parallel surgical robot with visual recognition, a double-layer isolation door, and a third intelligent control cabinet connected to each mechanism.

4. The intelligent system for the entire process of asexual tissue culture propagation of Reed japonica plants according to claim 1, characterized in that, Both the constant temperature and humidity induction culture room and the rooting culture room are equipped with multi-layer intelligent monitoring three-dimensional culture racks, constant temperature and humidity intelligent control systems and their control cabinets, supporting inspection robots and edge computing systems; each layer of the multi-layer intelligent monitoring three-dimensional culture racks holds breeding trays containing breeding cups; the edge computing system controls the multi-layer intelligent monitoring three-dimensional culture racks, constant temperature and humidity intelligent control systems and their control cabinets, and inspection robots via 5G and MES, and controls the culture room temperature to 25±2℃, the light intensity to 2500±500 lux, and the light cycle to 16 hours of light and 8 hours of darkness; The humid and warm nursery is equipped with a sensor network, intelligent actuators, an intelligent water and fertilizer integration system, inspection robots, and an edge computing platform. The sensor network is used to monitor the temperature and humidity of the substrate and the pH / EC value of the soil in real time. The intelligent actuators are used to control the skylights, fans, wet curtains, insulation curtains, supplemental lighting, sprinkler and drip irrigation, and fertilizer equipment. The inspection robots are equipped with multispectral and hyperspectral cameras, lidar, air temperature and humidity sensors, light intensity sensors, CO2 concentration sensors, automatic charging and battery swapping stations, laser weeding devices, insecticide spraying devices, a 5G cloud communication system, a robot intelligent navigation and control system, a Beidou positioning and navigation module, and an electronic map of the nursery. The sunroom is equipped with a movable intelligent shading film cover, an intelligent movable self-regulating water and fertilizer spraying cart, shading curtains, and an intelligent ventilation control system.

5. The intelligent system for the entire process of asexual tissue culture propagation of Reed japonica plants according to claim 1, characterized in that, The three-level biological germplasm bank includes: A first-class cryogenic -196°C liquid nitrogen preservation seed bank is equipped with cryogenic insulated liquid nitrogen storage tanks and their maintenance and monitoring systems; A secondary low-temperature 4°C tissue culture backup library, equipped with a multi-power supply cooling system and a circulating sterilization system; The three-level ambient temperature propagation chamber is equipped with a constant temperature and humidity system and a circulating disinfection system.

6. The fully intelligent system for the asexual tissue culture propagation of Reed japonica plants according to claim 1, characterized in that, In the material storage and transfer unit, the external seed source bank includes an external intelligent seed cache bank, an intelligent disinfection and inoculation consumables and accessories automated storage and retrieval system; the intelligent AGV cart is an intelligent AGV cart with robotic arm and lifting function, used for stacking, recycling, loading, unloading, transferring, filling and cleaning of seedlings, breeding cups, breeding trays and consumables between units; The cleaning, disinfection, and culture medium workshop is equipped with AGV trolleys with loading and unloading robotic arms, a fully automatic cup and plate cleaning and disinfection line, a culture medium filling line, culture medium storage tanks and a dispensing and metering pumping system, as well as a warehouse for vulnerable parts and consumables.

7. The intelligent system for the entire process of asexual tissue culture propagation of Reed japonica plants according to claim 1, characterized in that, The tissue culture seedling transplanting room is equipped with a root seedling transplanting robot, a transplanting preparation line with nutrient soil storage and quantitative addition functions, seedling trays, nursery transfer, loading and unloading robots, and a space circulation disinfection system. The seedling trays are made of biodegradable biomass fiber and slow-release nutrient material after final transplanting. The surface of the trays has a micro QR code that corresponds to the QR code of the breeding tray at each stage of the breeding process and the timeline. This QR code can be recognized by relevant robots, intelligent planting and transplanting machines, and edge computing in five stages: greenhouse seedling cultivation, sunshade seedling cultivation and hardening, transportation, on-site hardening, and on-site planting. The intelligent transplanter has intelligent speed regulation and steering self-walking function, Beidou positioning and navigation function, electronic map positioning and navigation function for planting plots, automatic hole digging function, root establishment water and fertilizer application function, soil covering and film covering function, and is equipped with a robotic arm for stacking seedling hole cups and trays, cutting seedlings, removing hole cups, and placing hole cups. Among the intelligent field equipment: the robot dog is equipped with a working manipulator, a visual recognition system, a laser weeding mechanism, a leaf-picking mechanism, a bird-driving voice control device, and a Beidou navigation module. It is a four-legged heavy-duty working robot used for leaf picking and weeding, tunnel network deployment and retrieval, planting and breeding patrols, security and grazing operations, and regular random sampling; the sampling and transportation drone is used to transport the samples collected by the robot dog to the molecular biology laboratory; the soil sensors include fixed soil sensors that are systematically installed together with intelligent weather stations and wind power and photovoltaic intelligent charging and swapping stations, as well as mobile soil sensors that are carried and charged by the robot dog for fixed-point and regular field testing.

8. The fully intelligent system for the asexual tissue culture propagation of Reed japonica plants according to claim 1, characterized in that, The multiple edge cloud brains deployed in the edge computing and decision-making layer specifically include: Edge cloud brains deployed in wind power and photovoltaic smart charging and swapping stations, edge cloud brains deployed in molecular biology laboratories, edge cloud brains deployed in tertiary biological seed banks, edge cloud brains deployed in cleaning, disinfection and culture medium workshops, edge cloud brains deployed in external seed and consumable material banks, edge cloud brains deployed in smart disinfection and inoculation rooms, edge cloud brains deployed in constant temperature and humidity induction culture rooms and constant temperature and humidity rooting culture rooms, edge cloud brains deployed in tissue culture seedling transplanting rooms, edge cloud brains deployed in greenhouse nurseries, edge cloud brains deployed in sun sheds, and edge cloud brains deployed in various processing bases; Each edge cloud brain integrates an industrial gateway, a supercomputing RTK base station, an edge node server, a blockchain node database, and various communication modules for loading lightweight AI models. Slow data is transmitted between the edge cloud brain and the data acquisition and execution layer devices using NB-IoT / 4G collaborative communication.

9. The fully intelligent system for the asexual tissue culture propagation of Reed japonica plants according to claim 1, characterized in that, The supercomputing data center for breeding, seedling cultivation, and planting includes: data analysis AI software, a knowledge graph prediction model library, a smart agriculture management system backend, a breeding storage database, a seedling database, and a planting tracking cloud database. It is used to optimize and publish seed selection, breeding, and seedling cultivation models, growth and biomass composition prediction models, disaster and pest models, carbon sequestration models, and digital twin models. Through the hash values, metadata, ownership certificates, and transaction logs of key data, it links with data centers of various traditional Chinese medicine planting and breeding alliance chains, energy biomass alliance chains, and raw material biomass alliance chains to store traceable and immutable data assets. Furthermore, through germplasm resource registration contracts, breeding data storage contracts, planting process record contracts, quality traceability query contracts, and data authorization access contracts, it links with various edge cloud brains, providing end-to-end data and decision support for user-level scientific research cooperation service providers, breeding and seedling producers, planting and plant protection field management service providers, reed asset owners, inter-row planting and breeding contractors, harvesting and transportation service providers, primary processing service providers, deep processing enterprises, end-user customers, government management departments, investment institutions, and financial institutions' apps or interfaces.

10. A fully intelligent method for the entire process of asexual tissue culture propagation of Reed japonica, based on the fully intelligent system for the entire process of asexual tissue culture propagation of Reed japonica as described in any one of claims 1-9, characterized in that, Includes the following steps: S1. Seed collection and planting: Collect superior Reed germplasm resources from different ecological zones and plant them in experimental fields in different climate zones. Each single plant forms an asexual line. S2. Intelligent data collection for seed selection: Using an intelligent drone platform, every 3 hours from 6 am onwards during the two growth periods, the drone automatically collects phenotypic data of the target nursery or field Reed plants over a large area and at high throughput. The data is then transmitted via 5G and the cloud to the target nursery or field via high throughput. The data includes height, diameter, crown width, biomass, chlorophyll content, nitrogen status, carbon dioxide and oxygen concentration. The data is then sent to the breeding, seedling cultivation and planting supercomputing data center. S3. Gene Analysis and Target Seed Source Screening: The supercomputing data center for breeding, seedling cultivation, and planting analyzes phenotypic data, integrates environmental data, and uses artificial intelligence and machine learning algorithms for data analysis to establish predictive models and accurately screen the best and superior clone target seed sources; in the molecular biology laboratory, new shoots of the target seed sources are collected, and DNA molecular marker maps are established through automated nucleic acid extraction devices and high-throughput gene sequencing devices to perform molecular marker-assisted selection and molecular detection marker monitoring; S4. Seedling disinfection treatment: The selected target seedlings are sent to an external intelligent seedling buffer, and then transported by an intelligent AGV to the negative pressure sterile disinfection box in the intelligent disinfection and inoculation room. In the negative pressure sterile disinfection box, a fully automated programmed disinfection is performed by a single-arm intelligent robotic arm: 70% alcohol surface immersion disinfection for 30 seconds → sterile distilled water rinsing twice → 2% NaClO solution with 1 drop of Tween-80 for main disinfection for 10 minutes → sterile distilled water rinsing five times for 1 minute each time → transfer by robotic arm. S5. Pruning, Inoculation, and Propagation: After disinfection, the seedlings are transferred to the intelligent pruning and inoculation box by an intelligent AGV cart. Using a visually-enabled intelligent parallel surgical robot, sterile forceps and a scalpel are used to remove the outer bracts and cut 1mm bud tips or stem tips or axillary buds with a small amount of tissue. These are then inoculated into MS induction medium containing cytokinins in cleaned and disinfected breeding cups to induce the formation of clustered buds. The clustered buds are then monitored by molecular detection and labeling. The intelligent AGV cart then places the breeding tray containing the breeding cups onto a multi-layer intelligent monitoring three-dimensional culture rack in the constant temperature and humidity induction culture room, where clustered buds are cultivated under the control of the corresponding edge cloud brain. S6. Rooting and Cultivation: Healthy bud clusters are transferred to an intelligent pruning and inoculation box. A smart parallel surgical robot with visual recognition identifies the healthy bud clusters and inoculates them onto clean, sterilized breeding cups containing auxin-containing MS rooting medium to induce the formation of a complete root system. The inoculated breeding cups are then transferred to a multi-layered intelligent monitoring three-dimensional culture rack in a constant temperature and humidity rooting culture chamber. Under the control of the corresponding edge cloud brain, seedlings are cultivated. Control conditions include: temperature 25±2℃, light intensity 2500±500 lux, and a photoperiod of 16 hours of light and 8 hours of darkness. S7. Hardening off and Transplanting in Seedling Trays: The breeding trays for root cultivation are moved into the tissue culture seedling transplanting room, and the breeding cups are opened for hardening off for 3 days. The root seedlings are removed from the breeding cups by the root seedling transplanting robot, and the culture medium is washed with sterile distilled water and then separated into individual plants. The individual root seedlings are placed one by one into the seedling trays on the transplanting preparation line and nutrient soil is added. The surface of the seedling trays is marked with a QR code associated with the QR code information of the breeding cups. The seedling trays are then transported to the greenhouse nursery by the nursery transfer loading and unloading robot. S8. Intermediate Processing: Recycling and processing seedling cups, breeding trays, induction medium, rooting medium, and preserving germplasm; using a fully automated cup and tray cleaning and disinfection line to clean and disinfect breeding cups and trays; using a culture medium dispensing line to automatically prepare and dispense culture medium, adding induction medium or rooting medium to the breeding cups in the trays; using a primary ultra-low temperature (-196°C) liquid nitrogen preservation germplasm bank to preserve the most robust clustered shoots, a secondary low temperature (4°C) tissue culture backup bank to rotate and back up the preservation of robust propagation clustered shoots annually, and a tertiary room temperature propagation working bank to preserve or cache germplasm for daily research and propagation; samples preserved in the tertiary biological germplasm bank are monitored by molecular detection and labeling. S9. Greenhouse Seedling Acclimation: In a greenhouse nursery, seedlings are placed for 10 days in an environment with a temperature of 23±5℃, humidity of 90±5% and shading rate of 70% for the first 4 days, humidity of 75±5% and shading rate of 50% for the next 6 days, and mist spraying without water accumulation. During this period, environmental parameters are monitored in real time through a sensor network, environmental adjustment equipment is controlled by intelligent actuators, inspection robots perform uninterrupted inspections, and local decision-making and control are performed by the corresponding edge cloud brain. S10. Cultivating and Hardening Off Seedlings in Sunrooms: After the seedlings have recovered from transplant shock, transfer the seedling trays and pots to a sunroom. Cultivate strong seedlings for 4 weeks and harden them off for 1 week in an environment with a temperature not lower than 15℃. During the strong seedling stage, the shading rate is 50% when the seedlings are first moved in, and then reduced by 10% each week. Use ventilation to control the day-night temperature difference to 10℃ and control the humidity to 60%. Ventilate for 2 hours each at noon and midnight every day, and spray diluted liquid fertilizer once a week. During the hardening off stage, provide full sun exposure in the open air without water or fertilizer and spray with mist for 1 week. S11. On-site hardening-off and transplanting: The seedling trays containing the seedlings to be transplanted are laid flat under a transparent rain shelter at the planting site for one week of on-site hardening-off. On the deeply tilled and ridged planting plots, intelligent planting and transplanting machines are used for transplanting. The intelligent planting and transplanting machines automatically locate and navigate based on Beidou positioning and navigation and the electronic map of the planting plot, and perform operations such as digging holes, removing seedling trays, placing seedling trays, applying water and fertilizer for root establishment, covering with soil, and covering with film. After transplanting, the seedlings will recover for 2 weeks until new buds emerge, and the seedlings will have survived. S12. Field Management and Data Collection: After transplanting, a smart drone platform is used to regularly collect phenotypic data of reeds; climate data is collected through an on-site smart weather station, and soil moisture, EC value, pH value, and NPK data are collected through an on-site soil sensor network; robotic dogs are used for weeding and leaf removal between rows, tunnel network deployment and retrieval, planting and breeding patrols, and security and grazing operations, and random samples are taken from the leaves picked between rows at regular intervals; the samples collected by the robotic dogs are transported by sampling and transport drones to the molecular biology laboratory for gene sequencing to obtain gene tracking and traceability data; the charging and swapping operations of the above-mentioned drones and robotic dogs, as well as the power supply of the smart weather station and soil sensors, are all provided by the wind power and photovoltaic smart charging and swapping station; S13. Data Upload and Edge Decision-Making: Data collected at each stage is transmitted to the edge cloud brain of the respective domain via NB-IoT / 4G / 5G; the edge cloud brain processes the real-time data, executes local decisions, controls the facilities, equipment, robots, smart agricultural machinery and sensor networks of the respective domain, and issues operation instructions, operation paths and prescription maps; the edge cloud brain uploads key events, aggregated data and summaries to the breeding, seedling and planting supercomputing data center via satellite network / 5G / 4G / WIFI, and receives the issued optimization strategies and AI model updates; S14. Harvesting and Data Traceability: After the reeds mature, they are harvested and packaged by intelligent harvesters, and the harvesting data and package marking data are uploaded to the edge cloud brain of the wind power and photovoltaic intelligent charging and swapping station. The edge cloud brain combines the harvesting data with the original traceability data to form a traceability data chain, which is then uploaded to the breeding, seedling cultivation and planting supercomputing data center. The supercomputing data center, through data cleaning, sorting, aggregation and blockchain encryption links, links the hash values, metadata, ownership certificates and transaction logs of key data with the data centers of various Chinese herbal medicine planting and breeding alliance chains, various energy biomass alliance chains, and various raw material biomass alliance chains, storing them to form traceable and immutable data assets. S15. Data Application and Decision Support: Through germplasm resource registration contracts, breeding data storage contracts, planting process record contracts, quality traceability query contracts, and data authorization access contracts, and by linking with the apps or interfaces of user-level scientific research cooperation service providers, breeding and seedling suppliers, planting, plant protection and field management service providers, reed asset owners, harvesting and transportation service providers, processing service providers, end customers, government management departments, and investment and financial institutions, we provide full-process data and decision support.

Images