An apparatus for assisting a neurotoxicity test in an animal

Abstract

The application discloses an animal in-vivo neurotoxicity auxiliary test device, and belongs to the technical field of biological tests.The device comprises an observation cage and a monitoring module, wherein the monitoring module is fixedly installed on the top of the observation cage; an auxiliary test mechanism is installed in the observation cage; the auxiliary test mechanism comprises a transfer assembly, a sealing assembly, a driving mechanism, a rotating frame, a lifting frame and an adjustable fixing mechanism for fixing a to-be-tested animal and adjusting the angle of the to-be-tested animal; the adjustable fixing mechanism is installed on the lifting frame; the lifting frame is slidingly connected with the rotating frame; the rotating frame is located in the sealing assembly; one end of the rotating frame, which is close to the driving mechanism, is connected with the transfer assembly; the sealing assembly, the rotating frame and the lifting frame are drivingly connected with the driving mechanism; the driving mechanism is installed in the transfer assembly; and the transfer assembly is installed in the opening of the observation cage. Through the above manner, the data reliability of the neurotoxicity test, the operation convenience and the comfort of the animal are improved.

Description

Technical Field

[0001] This invention relates to the field of biological testing technology, specifically to an auxiliary testing device for neurotoxicity in animals. Background Technology

[0002] Neurotoxicity refers to harmful changes in the structure or function of the nervous system caused by exposure to chemical, biological, or physical factors. Acute neurotoxicity testing is a core component of chemical safety evaluation, preclinical pharmacological studies of drugs, and environmental pollutant risk assessment. Currently, an internationally recognized acute neurotoxicity evaluation system has been established, centered on functional observational battery (FOB) testing, motor activity testing, and neuropathological examination.

[0003] Functional observation tests typically include five main categories: cage observation, hand observation, open-field observation, manipulative testing, and physiological index measurement. Specifically, cage observation primarily records spontaneous activity, posture, respiration, and seizures; hand observation assesses neuromuscular function such as muscle tone, grasping reflex, and tactile response; open-field observation evaluates motor ability, gait, and exploratory behavior; manipulative testing includes quantitative measurements such as forelimb grip strength and hindlimb support strength; and physiological index measurement includes body temperature, heart rate, and respiratory rate.

[0004] Currently, functional observation experiments still rely on manual operation. The specific operation process is as follows: the experimenters take the experimental animals out of the cages at predetermined time points and perform multiple operations in sequence, including cage observation, hand observation, and open-field observation. In the hand observation stage, the experimenters need to grasp the animals by hand and hold them in different body positions such as supine, prone, and lateral to observe and score items such as muscle tension, grasping reflex, and tactile response.

[0005] However, the existing manual operation methods have the following prominent problems in actual experiments: (1) Animal fixation is mostly done by firmly binding, which makes it impossible to quickly switch between multiple body positions. When changing the observation items, the experimenters need to repeatedly grab and adjust the animal's body position, which is cumbersome and easily causes stress response in the animals, affecting the accuracy of the observation results.

[0006] (2) Existing sealed dosing boxes only have the function of administering drugs and do not have the ability to conduct multi-angle behavioral observation and body position adjustment in a sealed state. After administering drugs, the experimenters need to take the animals out of the sealed device before they can be observed. This transfer process not only interrupts the continuity of drug administration, but may also lead to interruption of drug exposure or secondary contamination.

[0007] Based on this, the present invention designs an auxiliary testing device for neurotoxicity in animals to solve the above problems. Summary of the Invention

[0008] In view of the above-mentioned shortcomings of the existing technology, the present invention provides an auxiliary testing device for neurotoxicity in animals.

[0009] To achieve the above objectives, the present invention provides the following technical solution:

[0010] An auxiliary testing device for neurotoxicity in animals includes an observation cage and a monitoring module, wherein the monitoring module is fixedly installed on the top of the observation cage;

[0011] An auxiliary testing mechanism is installed inside the observation cage. The auxiliary testing mechanism includes a transfer component, a sealing component, a drive mechanism, a rotating frame, a lifting frame, and an adjustable fixing mechanism for fixing the animal to be tested and adjusting the angle of the animal. The adjustable fixing mechanism is installed on the lifting frame, which is slidably connected to the rotating frame. The rotating frame is located inside the sealing component, and the end of the rotating frame near the drive mechanism is connected to the transfer component. The sealing component, the rotating frame, and the lifting frame are all connected to the drive mechanism. The drive mechanism is installed inside the transfer component, which is installed inside the opening of the observation cage.

[0012] Furthermore, the transfer assembly includes an outer chamber, an electronic control chamber, an end cover plate, and a first electric push rod. The first electric push rod is fixedly installed inside the observation cage. The end cover plate is fixedly installed at the output end of the first electric push rod. The outer chamber is fixedly installed between the electronic control chamber and the end cover plate. The drive mechanism is installed inside the electronic control chamber. The sealing assembly is distributed inside the outer chamber. The outer chamber is slidably connected to the opening of the observation cage.

[0013] Furthermore, the sealing assembly includes an inner chamber, a first central tube, and a first transmission gear. The first central tube is fixedly installed at both ends of the inner chamber, the first central tube is rotatably connected to the outer chamber, and the first transmission gear is fixedly installed on the surface of the first central tube.

[0014] Furthermore, the drive mechanism includes a drive unit and an adjustable transmission unit that can be driven to and connected to the first transmission gear, the rotating frame and the lifting frame. The drive unit is installed inside the electrical control compartment, and the adjustable transmission unit is connected between the outer compartment and the electrical control compartment.

[0015] Furthermore, the drive unit includes a drive component, a first transmission tube, and a driven gear. The drive component is fixedly installed inside the electrical control compartment, the first transmission tube is rotatably connected inside the electrical control compartment, the driven gear is fixedly installed on the surface of the first transmission tube, and the drive component is drively connected to the driven gear.

[0016] Furthermore, the adjustable transmission unit includes a second transmission tube, a third rotating gear, a first key bar, a second key bar, a second transmission gear, a fourth transmission gear, a second electric push rod, and a push plate. The first key bar and the second key bar are fixedly installed on the surface of the second transmission tube. The second transmission gear is fixedly installed at one end of the second transmission tube near the lifting frame. A sleeve is fixedly installed on the surface of the third rotating gear. The first key bar can slide and connect with the first groove on the inner wall of the first transmission tube and the sleeve. The sleeve is rotatably connected to the surface of the push plate. The second key bar and the first key bar are distributed on both sides of the third rotating gear. The second electric push rod is fixedly installed in the electric control compartment. The push plate is fixedly installed at the output end of the second electric push rod. The fourth transmission gear is driven and connected in the electric control compartment. The third rotating gear can mesh with one side of the fourth transmission gear, and the first transmission gear meshes with the other side of the fourth transmission gear.

[0017] Furthermore, a second central tube is fixedly installed at one end of the rotating frame near the first transmission tube. The second central tube passes through the first central tube and is rotatably connected to the inner wall of the electrical control compartment. The second transmission tube passes through the second central tube. A sliding groove is provided on the inner wall of the first transmission tube. The second key bar can be slidably connected in the sliding groove. A longitudinal slide rail is fixedly installed on the inner wall of the rotating frame.

[0018] Furthermore, a slider is fixedly installed on the lifting frame near the longitudinal slide rail, and the slider is slidably connected in the longitudinal slide rail. A rack is fixedly installed on the side of the lifting frame near the first transmission tube, and the second transmission gear can mesh with the rack. The adjustable fixing mechanism is installed above the lifting frame.

[0019] Furthermore, the adjustable fixing mechanism includes a threaded rod, a movable frame, a second adjusting component, a fixing tube for fixing the animal to be tested, and a test platform for adjusting the angle of the animal to be tested. The fixing tube is fixedly installed above the test platform through the second adjusting component. The test platform is rotatably connected to a rotating shaft fixedly installed above the movable frame. The lower part of the movable frame is threadedly connected to a threaded groove on the surface of the threaded rod. A knob is fixedly installed at one end of the threaded rod. The threaded rod is rotatably connected to the inner side of the lifting frame. The surface of the fixing tube has a first limiting hole and a second limiting hole for placing the limbs of the animal to be tested. The two ends of the fixing tube are slidably connected to a first adjusting component. A pressure plate for fixing the animal to be tested is fixedly installed at the lower end of the first adjusting component.

[0020] Furthermore, the auxiliary testing mechanism also includes an atomizing pump for delivering atomized drugs to the test animal. The atomizing pump is fixedly installed in the electrical control chamber, and a delivery pipe is fixedly installed at the outlet end of the atomizing pump. The delivery pipe is rotatably connected to the end of the second transmission pipe that is away from the second transmission gear.

[0021] Compared with existing technologies, the advantages of this invention are as follows: Through the design of the transfer component, this invention can automatically control the opening of the observation cage for the test animal, precisely move a specific body part of the test animal directly below the operating instrument, and effectively prevent drug gas leakage through the combination of the sealing component and the drive mechanism. The combination of the rotating frame and the drive mechanism facilitates the monitoring module's collection of body reaction data from different angles. The combination of the lifting frame and the drive mechanism allows for precise adjustment of the test animal to a height matching the operating instrument during operations requiring precise positioning, such as intracranial injection. The adjustable fixing mechanism facilitates observation of the test animal's behavior in different body positions and, in some special drug administration methods, helps the animal maintain a specific posture. This invention allows for rapid switching between multiple body positions, eliminating the need for repeated handling and adjustment of the animal's position when changing observation items, and eliminating the need to remove the animal from the sealed device after drug administration for observation. This reduces the likelihood of animal stress reactions and improves the reliability of neurotoxicity test data, ease of operation, and animal comfort. Attached Figure Description

[0022] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are merely some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without any creative effort.

[0023] Figure 1 This is a perspective view of an auxiliary testing device for neurotoxicity in animals according to the present invention;

[0024] Figure 2 This is a first perspective view of the auxiliary testing mechanism of the present invention;

[0025] Figure 3 This is a partial cross-sectional view of the auxiliary testing mechanism of the present invention;

[0026] Figure 4 This is a partial perspective view of the auxiliary testing mechanism of the present invention;

[0027] Figure 5 This is a perspective view of the driving mechanism of the present invention;

[0028] Figure 6 This is a cross-sectional view of the auxiliary testing mechanism of the present invention;

[0029] Figure 7 For the present invention Figure 6 A magnified view of a portion of point a;

[0030] Figure 8For the present invention Figure 6 A magnified view of a section at point b in the middle;

[0031] Figure 9 This is a second perspective view of the auxiliary testing mechanism of the present invention.

[0032] Figure 10 This is a perspective view of the adjustable fixing mechanism of the present invention.

[0033] The labels in the diagram represent:

[0034] 1. Observation cage; 2. Outer compartment; 21. Electrically controlled compartment; 22. End cover plate; 23. First electric push rod; 3. Inner compartment; 31. First central tube; 32. First transmission gear; 4. Drive mechanism; 41. Drive component; 42. First transmission tube; 421. Driven gear; 43. Second transmission tube; 431. First key bar; 432. Second key bar; 433. Second transmission gear; 44. Second electric push rod; 45. Push plate; 46. Third rotating gear; 47. Fourth transmission gear; 5. 51. Rotating frame; 52. Longitudinal slide rail; 53. Second central tube; 54. Slide groove; 55. Lifting frame; 66. Sliding block; 67. Rack; 78. Adjustable fixing mechanism; 79. Fixing tube; 70. Pressure plate; 712. First adjusting component; 713. First limiting hole; 714. Second limiting hole; 72. Test platform; 73. Rotating shaft; 74. Moving frame; 75. Threaded rod; 76. Knob; 77. Second adjusting component; 8. Nebulizing pump; 88. Delivery pipe; 9. Monitoring module. Detailed Implementation

[0035] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0036] Example 1: In some embodiments, please refer to the accompanying drawings. Figures 1-10 An auxiliary testing device for neurotoxicity in animals includes an observation cage 1 and a monitoring module 9, wherein the monitoring module 9 is fixedly installed on the top of the observation cage 1;

[0037] An auxiliary testing mechanism is installed inside the observation cage 1. The auxiliary testing mechanism includes a transfer component, a sealing component, a drive mechanism 4, a rotating frame 5, a lifting frame 6, and an adjustable fixing mechanism 7 for fixing the animal to be tested and adjusting the angle of the animal to be tested. The adjustable fixing mechanism 7 is installed on the lifting frame 6. The lifting frame 6 is slidably connected to the rotating frame 5. The rotating frame 5 is located inside the sealing component. The end of the rotating frame 5 near the drive mechanism 4 is connected to the transfer component. The sealing component, the rotating frame 5, and the lifting frame 6 are all connected to the drive mechanism 4. The drive mechanism 4 is installed inside the transfer component. The transfer component is installed inside the opening of the observation cage 1.

[0038] In this embodiment, when the in vivo neurotoxicity auxiliary testing device is working normally, the transfer component can automatically control the opening of the observation cage 1 for the test animal to enter and exit. The sealing component, in conjunction with the drive mechanism 4, allows for free rotation of the sealing component, sealing the test animal between the sealing component and the transfer component. This effectively prevents drug gas leakage, avoiding environmental pollution and protecting the health of laboratory personnel. It also prevents leakage when anesthetic gas or nebulized drugs need to be introduced. The rotating frame 5, in conjunction with the drive mechanism 4, allows adjustment of the observation angle of the test animal around the axis of the sealing component, facilitating the monitoring module 9 to collect data on the animal's physical responses at different angles. The lifting frame 6, in conjunction with the drive mechanism 4, allows adjustment of the height of the test animal within the sealing component, enabling precise positioning during operations requiring intracranial injection. The platform allows for precise adjustment of the test animal to a height that matches the operating instrument. The adjustable fixing mechanism 7 not only allows for manual fixing of the test animal but also manual adjustment of its tilt angle, facilitating observation of the animal's behavior in different body positions and ensuring animal comfort. Furthermore, in neurotoxicity tests, precise positioning procedures are often required, such as intracranial micro-injection, blood sampling, and EEG electrode implantation. By adjusting the tilt angle, a specific body part of the test animal (such as the head or back) can be precisely moved directly below the operating instrument (such as a stereotaxic instrument or micro-injection pump). In some special drug administration methods (such as intrathecal injection or intraventricular injection), the animal needs to maintain a specific posture (such as spinal curvature). The platform tilt function can assist in achieving these special posture requirements, thereby improving the data reliability, operational convenience, and animal comfort in neurotoxicity tests.

[0039] Example 2: In some embodiments, such as Figures 1-10As shown, in a preferred embodiment of the present invention, the transfer assembly includes an outer chamber 2, an electrically controlled chamber 21, an end cover plate 22, and a first electric push rod 23. The first electric push rod 23 is fixedly installed inside the observation cage 1, the end cover plate 22 is fixedly installed at the output end of the first electric push rod 23, the outer chamber 2 is fixedly installed between the electrically controlled chamber 21 and the end cover plate 22, the drive mechanism 4 is installed inside the electrically controlled chamber 21, the sealing assembly is distributed inside the outer chamber 2, and the outer chamber 2 is slidably connected to the opening of the observation cage 1.

[0040] The sealing assembly includes an inner chamber 3, a first central tube 31, and a first transmission gear 32. The first central tube 31 is fixedly installed at both ends of the inner chamber 3. The first central tube 31 is rotatably connected to the outer chamber 2. The first transmission gear 32 is fixedly installed on the surface of the first central tube 31.

[0041] In this embodiment, the first electric push rod 23 can control the opening of the outer chamber 2 to enter and exit the observation cage 1 through the setting of the outer chamber 2. Both the outer chamber 2 and the inner chamber 3 are made of transparent material, which facilitates the collection and observation of the behavioral data and actions of the animal to be tested. The inner chamber 3 slides in contact with the inner wall of the outer chamber 2. When the inner chamber 3 rotates to the top of the outer chamber 2, the two can form a sealed cavity, which can effectively prevent the leakage of drug gas. An annular seal (not marked in the figure) is fixedly installed between the inner wall of the outer chamber 2 and the outer wall of the inner chamber 3. The annular seal is made of medical grade silicone material to ensure the reliability of the seal.

[0042] The monitoring module 9 records key behavioral data of the animal under test, such as limb movements, respiratory rate, and seizure response, through the transparent outer chamber 2 and inner chamber 3. The monitoring module 9 includes at least two sets of high-speed camera units, fixedly installed at different positions on the top of the observation cage 1, for simultaneously acquiring behavioral image data of the animal under test from different angles. The monitoring module 9 also includes an infrared supplementary lighting unit to provide uniform illumination under low-light conditions to ensure image acquisition quality. The image data acquired by the monitoring module 9 is transmitted to an external computer for analysis and processing via wired or wireless means.

[0043] Example 3: In some embodiments, such as Figures 2-10 As shown, in a preferred embodiment of the present invention, the drive mechanism 4 includes a drive unit and an adjustable transmission unit that can be driven to and transmitted with the first transmission gear 32, the rotating frame 5 and the lifting frame 6. The drive unit is installed inside the electric control compartment 21, and the adjustable transmission unit is connected between the outer compartment 2 and the electric control compartment 21.

[0044] The drive unit includes a drive component 41, a first transmission tube 42, and a driven gear 421. The drive component 41 is fixedly installed inside the electrical control compartment 21. The first transmission tube 42 is rotatably connected inside the electrical control compartment 21. The driven gear 421 is fixedly installed on the surface of the first transmission tube 42. The drive component 41 and the driven gear 421 are connected in a transmission manner. The drive component 41 includes a drive motor and a drive gear fixedly installed at the output end of the drive motor. The drive gear and the driven gear 421 are connected in a transmission manner.

[0045] The adjustable transmission unit includes a second transmission pipe 43, a third rotating gear 46, a first key bar 431, a second key bar 432, a second transmission gear 433, a fourth transmission gear 47, a second electric push rod 44, and a push plate 45. The first key bar 431 and the second key bar 432 are fixedly mounted on the surface of the second transmission pipe 43. The second transmission gear 433 is fixedly mounted on one end of the second transmission pipe 43 near the lifting frame 6. A sleeve is fixedly mounted on the surface of the third rotating gear 46. The first key bar 431 can engage with the first transmission pipe 42 and... The first groove on the inner wall of the sleeve is slidably connected, the sleeve is rotatably connected to the surface of the push plate 45, the second key bar 432 and the first key bar 431 are distributed on both sides of the third rotating gear 46, the second electric push rod 44 is fixedly installed in the electric control compartment 21, the push plate 45 is fixedly installed at the output end of the second electric push rod 44, the fourth transmission gear 47 is tractively connected in the electric control compartment 21, the third rotating gear 46 can mesh with one side of the fourth transmission gear 47, and the first transmission gear 32 meshes with the other side of the fourth transmission gear 47;

[0046] The rotating frame 5 has a second central tube 52 fixedly installed at one end near the first transmission tube 42. The second central tube 52 passes through the first central tube 31 and is rotatably connected to the inner wall of the electrical control compartment 21. The second transmission tube 43 passes through the second central tube 52. The inner wall of the first transmission tube 42 is provided with a sliding groove 521. The second key bar 432 can be slidably connected in the sliding groove 521. The inner wall of the rotating frame 5 is fixedly installed with a longitudinal slide rail 51.

[0047] A slider 61 is fixedly installed on the lifting frame 6 near the longitudinal slide rail 51. The slider 61 is slidably connected in the longitudinal slide rail 51. A rack 62 is fixedly installed on the side of the lifting frame 6 near the first transmission tube 42. The second transmission gear 433 can mesh with the rack 62. The adjustable fixing mechanism 7 is installed above the lifting frame 6.

[0048] In this embodiment, the second electric push rod 44 allows the push plate 45 to control the horizontal movement of the second transmission tube 43, the third rotating gear 46, the second transmission gear 433, and the second key bar 432. When the third rotating gear 46 meshes with the fourth transmission gear 47, the second key bar 432 disengages from the groove 521 in the second central tube 52, and the second transmission gear 433 disengages from the rack 62. When the drive member 41 controls the first transmission tube 42, the second transmission tube 43, and the second transmission gear 433 to rotate via the driven gear 421, the rotating second transmission tube 43 controls the rotation of the inner chamber 3 only via the third rotating gear 46 and the fourth transmission gear 47, thus regulating the opening and closing of the inner chamber 3. When the second key bar 432 enters the groove 521 in the second central tube 52, the third rotating gear 46 disengages from the fourth transmission gear 47, and the second transmission gear 433 disengages from the rack 62. When the second transmission tube 43 is still disengaged from the rack 62, and the drive unit 41 controls the second transmission gear 433 to rotate, the rotating second transmission tube 43 controls the rotating frame 5, the lifting frame 6, and the adjustable fixing mechanism 7 to rotate synchronously around the axis of the second central tube 52 and the first central tube 31 only through the second key bar 432 and the slide groove 521, thereby adjusting the observation angle of the animal to be tested. When the second transmission gear 433 is engaged with the rack 62, the second key bar 432 is disengaged from the slide groove 521, and the third rotating gear 46 is still disengaged from the fourth transmission gear 47. When the drive unit 41 controls the second transmission gear 433 to rotate, the rotating second transmission tube 43 controls the lifting frame 6 to move up and down along the longitudinal slide rail 51 only through the second transmission gear 433 and the rack 62, thereby adjusting the height of the animal to be tested in the inner chamber 3, and facilitating the adjustment of the posture and observation angle of the animal to be tested.

[0049] Example 4: In some embodiments, such as Figures 2-10 As shown, in a preferred embodiment of the present invention, the adjustable fixing mechanism 7 includes a threaded rod 74, a movable frame 73, a second adjusting member 76, a fixing tube 71 for fixing the animal to be tested, and a test platform 72 for adjusting the angle of the animal to be tested. The fixing tube 71 is fixedly installed above the test platform 72 via the second adjusting member 76, which is a bolt threaded between the fixing tube 71 and the test platform 72. The test platform 72 is rotatably connected to a rotating shaft 721 fixedly installed above the movable frame 73. The lower part of the frame 73 is threadedly connected to the threaded groove on the surface of the threaded rod 74. The two sides of the movable frame 73 are slidably connected to the inner wall of the lifting frame 6. A knob 75 is fixedly installed at one end of the threaded rod 74. The threaded rod 74 is rotatably connected to the inner side of the lifting frame 6. The surface of the fixed tube 71 is provided with a first limiting hole 713 and a second limiting hole 714 for placing the limbs of the animal to be tested. The two ends of the fixed tube 71 are slidably connected to a first adjusting member 712. A pressure plate 711 for fixing the animal to be tested is fixedly installed at the lower end of the first adjusting member 712.

[0050] In this embodiment, the setting of controlling the rotation of the threaded rod 74 by the knob 75 can control the horizontal movement of the moving frame 73 along the surface of the lifting frame 6. On the one hand, it serves to adjust the position of the fixed tube 71 in the inner chamber 3 according to the body length of the animal to be tested. On the other hand, it facilitates the precise movement of a certain body part of the animal to be tested, such as the head or back, directly under the operating instrument (such as a stereotaxic instrument or a micro-injection pump). The setting of manually adjusting the rotation of the test platform 72 around the rotating axis 721 can adjust the tilt angle of the test platform 72 and the fixed tube 71. This not only facilitates the observation of the behavior of the animal to be tested in different body positions, but also assists in the administration of drugs to specific locations of the animal to be tested.

[0051] The fixing tube 71 has pressure plates 711 at both ends and a first adjusting member 712. The first adjusting member 712 is a bolt or movable pin connected in the movable groove of the fixing tube 71. It is used to adjust the horizontal position or radial height of the pressure plate 711 according to the body size of the animal to be tested. The material of the contact surface between the pressure plate 711 and the animal to be tested is a flexible pad to avoid causing pressure damage to the animal to be tested. The second adjusting member 76 can not only disassemble and replace the fixing tube 71, but also adjust the position of the fixing tube 71 on the surface of the test platform 72. The inner diameter of the fixing tube 71 is suitable for test rats with a body length of 15~25cm and an inner diameter of 50~80mm. The front and rear pressure plates 711 can fix the head and tail of the animal to be tested respectively to prevent it from making violent movements due to stress response. The front and hind limbs of the animal to be tested can be placed into the second limiting hole 714 and the first limiting hole 713 respectively to prevent the limbs of the animal to be tested from being squeezed and damaged.

[0052] Example 5: In some embodiments, such as Figures 2-6 As shown, in a preferred embodiment of the present invention, the auxiliary testing mechanism further includes an atomizing pump 8 for delivering atomized drugs to the test animal. The atomizing pump 8 is fixedly installed in the electrical control chamber 21. A delivery pipe 81 is fixedly installed at the air outlet end of the atomizing pump 8. The delivery pipe 81 is rotatably connected to the end of the second transmission pipe 43 that is away from the second transmission gear 433.

[0053] In this embodiment, the nebulizer pump 8 can deliver anesthetic gas or nebulized drugs between the inner chamber 3 and the outer chamber 2 through the delivery pipe 81 and the second transmission pipe 43. The flow sensor integrated in the nebulizer pump 8 enables precise control of the drug concentration, preventing the test animal from inhaling too much drug. Optionally, a temperature and humidity sensor (the specific model is not limited) is fixedly installed in the inner chamber 3 or the outer chamber 2 to monitor the internal temperature, humidity and other parameters in real time. The flow control range of the nebulizer pump 8 is 0.1~10mL / min. The working modes include continuous drug delivery mode, pulse drug delivery mode and timed and quantitative drug delivery mode. The above drug delivery modes can be freely selected according to actual needs.

[0054] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. An auxiliary testing device for neurotoxicity in animals, comprising an observation cage (1) and a monitoring module (9), wherein the monitoring module (9) is fixedly installed on the top of the observation cage (1), characterized in that: An auxiliary testing mechanism is installed inside the observation cage (1). The auxiliary testing mechanism includes a transfer component, a sealing component, a drive mechanism (4), a rotating frame (5), a lifting frame (6), and an adjustable fixing mechanism (7) for fixing the animal to be tested and adjusting the angle of the animal to be tested. The adjustable fixing mechanism (7) is installed on the lifting frame (6). The lifting frame (6) is slidably connected to the rotating frame (5). The rotating frame (5) is located inside the sealing component. The end of the rotating frame (5) near the drive mechanism (4) is connected to the transfer component. The sealing component, the rotating frame (5), and the lifting frame (6) are all connected to the drive mechanism (4) in a transmission manner. The drive mechanism (4) is installed inside the transfer component. The transfer component is installed inside the opening of the observation cage (1).

2. The animal in vivo neurotoxicity auxiliary testing device according to claim 1, characterized in that, The transfer assembly includes an outer chamber (2), an electrical control chamber (21), an end cover plate (22), and a first electric push rod (23). The first electric push rod (23) is fixedly installed inside the observation cage (1). The end cover plate (22) is fixedly installed at the output end of the first electric push rod (23). The outer chamber (2) is fixedly installed between the electrical control chamber (21) and the end cover plate (22). The drive mechanism (4) is installed inside the electrical control chamber (21). The sealing assembly is distributed inside the outer chamber (2). The outer chamber (2) is slidably connected to the opening of the observation cage (1).

3. The animal in vivo neurotoxicity auxiliary testing device according to claim 2, characterized in that, The sealing assembly includes an inner chamber (3), a first central tube (31) and a first transmission gear (32). The first central tube (31) is fixedly installed at both ends of the inner chamber (3). The first central tube (31) is rotatably connected to the outer chamber (2). The first transmission gear (32) is fixedly installed on the surface of the first central tube (31).

4. The animal in vivo neurotoxicity auxiliary testing device according to claim 3, characterized in that, The drive mechanism (4) includes a drive unit and an adjustable transmission unit that can be connected to the first transmission gear (32), the rotating frame (5) and the lifting frame (6). The drive unit is installed inside the electric control compartment (21), and the adjustable transmission unit is connected between the outer compartment (2) and the electric control compartment (21).

5. The animal in vivo neurotoxicity auxiliary testing device according to claim 4, characterized in that, The drive unit includes a drive component (41), a first transmission tube (42), and a driven gear (421). The drive component (41) is fixedly installed inside the electrical control compartment (21). The first transmission tube (42) is rotatably connected inside the electrical control compartment (21). The driven gear (421) is fixedly installed on the surface of the first transmission tube (42). The drive component (41) and the driven gear (421) are connected in a transmission manner.

6. The animal in vivo neurotoxicity auxiliary testing device according to claim 5, characterized in that, The adjustable transmission unit includes a second transmission pipe (43), a third rotating gear (46), a first key bar (431), a second key bar (432), a second transmission gear (433), a fourth transmission gear (47), a second electric push rod (44), and a push plate (45). The first key bar (431) and the second key bar (432) are fixedly installed on the surface of the second transmission pipe (43). The second transmission gear (433) is fixedly installed at one end of the second transmission pipe (43) near the lifting frame (6). A sleeve is fixedly installed on the surface of the third rotating gear (46). The first key bar (431) can interact with the first transmission pipe (431). 2) The sleeve is slidably connected to the first groove on the inner wall of the sleeve. The sleeve is rotatably connected to the surface of the push plate (45). The second key bar (432) and the first key bar (431) are distributed on both sides of the third rotating gear (46). The second electric push rod (44) is fixedly installed in the electric control compartment (21). The push plate (45) is fixedly installed at the output end of the second electric push rod (44). The fourth transmission gear (47) is connected to the electric control compartment (21). The third rotating gear (46) can mesh with one side of the fourth transmission gear (47). The first transmission gear (32) meshes with the other side of the fourth transmission gear (47).

7. The animal in vivo neurotoxicity auxiliary testing device according to claim 6, characterized in that, The rotating frame (5) has a second central tube (52) fixedly installed at one end near the first transmission tube (42). The second central tube (52) passes through the first central tube (31) and is rotatably connected to the inner wall of the electrical control compartment (21). The second transmission tube (43) passes through the second central tube (52). The inner wall of the first transmission tube (42) is provided with a sliding groove (521). The second key bar (432) can be slidably connected in the sliding groove (521). The inner wall of the rotating frame (5) is fixedly installed with a longitudinal slide rail (51).

8. The animal in vivo neurotoxicity auxiliary testing device according to claim 7, characterized in that, A slider (61) is fixedly installed on the lifting frame (6) near the longitudinal slide rail (51). The slider (61) is slidably connected in the longitudinal slide rail (51). A rack (62) is fixedly installed on the side of the lifting frame (6) near the first transmission tube (42). The second transmission gear (433) can mesh with the rack (62). The adjustable fixing mechanism (7) is installed above the lifting frame (6).

9. The animal in vivo neurotoxicity auxiliary testing device according to claim 8, characterized in that, The adjustable fixing mechanism (7) includes a threaded rod (74), a movable frame (73), a second adjusting member (76), a fixing tube (71) for fixing the animal to be tested, and a test platform (72) for adjusting the angle of the animal to be tested. The fixing tube (71) is fixedly installed above the test platform (72) through the second adjusting member (76). The test platform (72) is rotatably connected to a rotating shaft (721) fixedly installed above the movable frame (73). The lower part of the movable frame (73) is threadedly connected to the threaded groove on the surface of the threaded rod (74). The movable frame (73) is slidably connected to the inner wall of the lifting frame (6) on both sides. A knob (75) is fixedly installed at one end of the threaded rod (74). The threaded rod (74) is rotatably connected to the inner side of the lifting frame (6). The surface of the fixed tube (71) is provided with a first limiting hole (713) and a second limiting hole (714) for placing the limbs of the animal to be tested. The two ends of the fixed tube (71) are slidably connected to a first adjusting member (712). A pressure plate (711) for fixing the animal to be tested is fixedly installed at the lower end of the first adjusting member (712).

10. The animal in vivo neurotoxicity auxiliary testing device according to claim 9, characterized in that, The auxiliary testing mechanism also includes an atomizing pump (8) for delivering atomized drugs to the test animals. The atomizing pump (8) is fixedly installed in the electrical control chamber (21). A delivery pipe (81) is fixedly installed at the outlet end of the atomizing pump (8). The delivery pipe (81) is rotatably connected to the end of the second transmission pipe (43) that is away from the second transmission gear (433).

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