A method for keeping scophthalmus maximus alive without water

Abstract

The application discloses a kind of scophthalmus maximus anhydrous keep-alive methods, belong to aquatic product fresh-keeping and keep-alive technical field.The method is through seawater temporary cultivation, temperature reduction treatment, narcotic treatment, electric shock treatment in proper order, and the scophthalmus maximus after electric shock treatment is placed into packaging bag alone, and the packaging bag is placed into low-temperature environment after oxygenation and is preserved and transported.This method introduces white magnolia essential oil in narcotic treatment process, significantly improves the survival rate of scophthalmus maximus anhydrous keep-alive transportation, and the application establishes green and efficient scophthalmus maximus anhydrous keep-alive transportation technology, which is of great significance to improve industrial transportation efficiency and ensure product safety and flavor quality.

Description

Technical Field

[0001] This invention belongs to the field of aquatic product preservation and live preservation technology, and particularly relates to a method for keeping turbot alive without water. Background Technology

[0002] Preservation of aquatic products is a crucial link between aquaculture production and end-consumer needs. It is of great significance for ensuring product freshness, maintaining their original flavor and nutritional value, and improving the economic benefits of the aquatic product industry chain. my country is the world's largest producer and consumer of aquatic products. Although some progress has been made in the preservation and transportation of aquatic products, it is constrained by core factors such as the lack of green and efficient stress mitigation methods and the imperfect system for full-process monitoring and stress control. In addition, the low penetration rate of specialized transportation equipment and insufficient technical standardization pose many practical challenges to the development of the industry.

[0003] Turbot (Scophthalmus maximus), commonly known as "turbot," is a major marine fish farmed in the coastal areas of northern my country. Its white flesh, delicious flavor, and high collagen content make it a recognized high-value edible fish in the international market, highly favored by consumers and possessing significant nutritional and economic value. In practice, long-distance or long-term transportation is often necessary, making research into live fish transport technology crucial. However, during long-distance transportation, temporary holding, and sales, live fish are susceptible to the combined effects of multiple environmental stressors, including temperature stress, vibration stress, hypoxia stress, and ammonia nitrogen accumulation. This leads to significant stress responses in the fish, causing dysfunction of the immune system and imbalance of the antioxidant defense system, further exacerbating oxidative damage and ultimately increasing mortality during transport. Simultaneously, stress also deteriorates muscle quality and causes the loss of flavor compounds, resulting in a product quality far lower than when freshly caught.

[0004] With consumers' increasing demands for food safety and quality, the market's demand for the freshness of aquatic products is growing stronger. Developing efficient, safe, and green high-quality preservation and live-keeping technologies has become an urgent need to promote the upgrading of my country's aquatic product industry. Traditional water-based transportation methods have drawbacks such as low loading capacity, easy water quality deterioration, high transportation costs, and high risk of pathogen transmission, making them unsuitable for large-scale, high-quality distribution needs. Waterless transportation refers to transporting aquatic products under waterless conditions after inducing a dormant or semi-dormant state through low temperatures or anesthesia. In this state, the aquatic products' metabolism is reduced, stress response is decreased, and mechanical damage during transportation is reduced. Waterless transportation eliminates the need for water, significantly reducing transportation costs and facilitating land or air transport, expanding the delivery range of fresh aquatic products, and significantly improving transportation efficiency. Waterless live-keeping transportation technology induces aquatic animals into a dormant state through environmental control, achieving high-density transportation under waterless conditions, demonstrating significant application potential in the aquatic product distribution sector. However, waterless transportation, due to multiple stresses such as dehydration and hypoxia, easily induces severe physiological stress in fish. If stress is not properly controlled, it can significantly increase fish mortality during transportation and exacerbate the deterioration of muscle quality. Therefore, researching a green preservation technology that can effectively alleviate transportation stress and achieve high survival rates and high quality is crucial for the live transportation of aquatic products.

[0005] Low-temperature dormancy refers to the controlled gradient cooling process used to lower the temperature of aquatic products to their ecological ice temperature range (between the critical temperature and the freezing point), minimizing metabolic rate and respiratory consumption, thus inducing a state of extreme metabolic inhibition, known as dormancy or semi-dormancy. Low-temperature dormancy can extend transportation time, improve survival rates, and ensure product quality. However, prolonged low-temperature stress can lead to excessive energy consumption, decreased muscle quality, and cold stress damage in fish, increasing transportation costs.

[0006] Anesthetic dormancy refers to the technique of using chemical or natural substances to inhibit the central nervous system of aquatic products, inducing a reversible state of sedation or anesthesia. This reduces metabolic intensity, oxygen consumption, and activity levels, minimizing transport stress and facilitating waterless transport operations. Currently, chemical anesthetics used in aquatic product transport mainly include ethyl m-aminobenzoate methanesulfonate (MS-222), 2-phenoxyethanol, benzocaine, and CO2. Due to safety and operational issues associated with these chemical anesthetics, finding safer and more effective natural alternatives has become a research hotspot in the field of fishery anesthesia.

[0007] Natural anesthetics generally refer to essential oils and their anesthetic compounds extracted from the flowers, leaves, stems, roots, or fruits of aromatic plants. In aquaculture and transportation, essential oils, due to their natural and easily degradable properties, can serve as alternatives to chemical anesthetics. The natural active ingredients in plants, after being absorbed through the gills or skin of fish, act on the central nervous system, interfering with ion channels in nerve cells, blocking action potential transmission, and regulating the neurotransmitter system, thereby inducing a reversible state of sedation or anesthesia in the fish. This can reduce stress and physical damage to fish during harvesting, sorting, vaccination, and transportation, effectively improving animal welfare and operational safety. Plant essential oils not only have anesthetic effects but also possess various physiological functions such as antioxidation, antibacterial properties, anti-inflammation, and immunomodulation. Magnolia denudata, as a traditional aromatic plant in my country, has a wide range of essential oil sources, unique aroma components, and low cost, making it uniquely valuable for development in the field of live-keeping and transportation. Summary of the Invention

[0008] In view of this, the present invention discloses a method for keeping turbot alive without water.

[0009] The present invention adopts the following technical solution: A method for keeping turbot alive without water, the method comprising the following steps: S1. Temporary seawater holding: Turbot is temporarily held in seawater. S2. Cooling treatment: The turbot temporarily held in seawater is cooled to obtain cooled turbot. S3. Anesthesia treatment: The turbot subjected to the cooling treatment was anesthetized for 5-10 minutes in an anesthesia tank using white magnolia essential oil seawater solution. S4. Electroshock treatment: Electroshock treatment is performed on the turbot after anesthesia. S5. Packaging: After the turbot has been electrocuted, it is placed in a separate packaging bag, which is then filled with oxygen and transported in a low-temperature environment.

[0010] Furthermore, the salinity of the seawater described in S1 is 28‰.

[0011] Furthermore, the seawater has a pH of 7.23, a water temperature of 14±1℃, and a dissolved oxygen content of 6.3-7.0 mg / L.

[0012] Furthermore, the temporary holding time is 24 hours.

[0013] Furthermore, the cooling process described in S2 is a gradient cooling process, and the cooling rate of the gradient cooling is 3℃ / h.

[0014] Furthermore, the endpoint of the gradient cooling is 4-6℃.

[0015] Further, the anesthesia treatment described in S3 includes: preparing a 100 mg / L-500 mg / L concentration of magnolia essential oil seawater solution in an anesthesia tank, cooling the 100 mg / L magnolia essential oil seawater solution, and then placing the cooled turbot into the anesthesia tank.

[0016] Furthermore, the endpoint of the cooling of the magnolia essential oil seawater solution is 4-6℃.

[0017] Furthermore, the voltage of the electric shock in S4 is 30~60V, the frequency of the electric shock is 10-50Hz, and the duration of the electric shock is 3s.

[0018] Furthermore, the low-temperature environment described in S5 is 3℃-5℃.

[0019] The present invention has the following advantages over the prior art: Waterless live transport has the advantages of low cost, high survival rate and low environmental impact, making it the preferred solution for long-distance transport of live turbot. By inducing fish into a dormant state through environmental regulation, the survival time can be effectively extended.

[0020] Magnolia is widely available and inexpensive. Magnolia essential oil has a unique aroma and is a natural plant component. Compared to chemical anesthesia, magnolia essential oil is safer and more effective, and has unique development value in the field of live animal transportation.

[0021] The synergistic application of white magnolia essential oil, which has calming, antibacterial, and antioxidant properties, and low-temperature dormancy technology can effectively alleviate stress and its impact on the physiological metabolism of fish, thereby improving muscle quality.

[0022] Combining natural plant ingredient magnolia essential oil with electroshock technology can effectively reduce the stress response of turbot.

[0023] By combining natural plant ingredient magnolia essential oil, electric shock physical technology, and low-temperature dormancy technology, operating costs are reduced and stress response in turbot is greatly minimized.

[0024] To enrich the theoretical system of live transportation and quality control of aquatic products, and to establish a green and efficient waterless live transportation technology for turbot, so as to provide technical support for improving the industry's transportation efficiency, ensuring product safety and flavor quality, and promoting the high-quality development of live transportation of aquatic products.

[0025] This invention combines natural plant ingredient magnolia essential oil, electroshock technology, and low-temperature dormancy technology for the waterless live transport of turbot. In particular, the combined use of magnolia essential oil and electroshock technology not only significantly improves the survival rate of turbot during waterless live transport but also effectively alleviates stress and its impact on the fish's physiological metabolism, improving the muscle quality of turbot. This live transport technology is of great significance for establishing a green and efficient waterless live transport technology for turbot, improving the transport efficiency of the turbot industry, ensuring the safety and flavor quality of turbot products, and promoting the high-quality development of live aquatic product transport. Attached Figure Description

[0026] To more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0027] Figure 1 This is a schematic diagram illustrating the survival rate of turbot samples at different times in an embodiment of the present invention. Figure 2 This is a schematic diagram illustrating the changes in cortisol content in the blood of turbot according to an embodiment of the present invention; Figure 3 This is a schematic diagram of the method for keeping turbot alive according to the present invention. Detailed Implementation

[0028] To better understand the technical solution of the present invention, the embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0029] It should be understood that the described embodiments are merely some embodiments of the present invention, and not all embodiments. Unless otherwise specified, the reagents or methods involved in the following embodiments are all conventional reagents or experimental methods in the art.

[0030] Example 1

[0031] S1. Select fresh, live turbot weighing 750 ± 100 g and place them in pre-prepared seawater with a salinity of 28‰, pH 7.23, water temperature of 14 ± 1 ℃, and dissolved oxygen of 6.3 - 7.0 mg / L for 24 hours to eliminate stress reactions during transportation.

[0032] S2. After temporarily holding turbot for 24 hours, a gradient cooling treatment was carried out by adding ice packs. The cooling rate was controlled at 2-3℃ / h. For every 1℃ decrease in temperature, the respiratory rate and behavioral status of the turbot were observed and recorded. The temperature was lowered to 4℃.

[0033] S3. Place the cooled turbot into a tank filled with seawater and subject it to electric shock. The electric shock voltage is 50V, the output frequency is 25Hz, and the electric shock time is 3s.

[0034] S4. After transportation, remove the turbot and place it in clean water at 12-15 ℃. The turbot will fully recover.

[0035] Example 2

[0036] S1. Select fresh, live turbot weighing 750 ± 100 g and place them in pre-prepared seawater with a salinity of 28‰, pH 7.23, water temperature of 14 ± 1 ℃, and dissolved oxygen of 6.3 - 7.0 mg / L for 24 hours to eliminate stress reactions during transportation.

[0037] S2. After temporarily holding turbot for 24 hours, a gradient cooling treatment was carried out by adding ice packs. The cooling rate was controlled at 2-3℃ / h. For every 1℃ decrease in temperature, the respiratory rate and behavioral status of the turbot were observed and recorded. The temperature was lowered to 4℃.

[0038] S3. A concentration of 200 mg / L of magnolia essential oil was prepared in an anesthesia tank based on the volume of seawater, and the seawater temperature was lowered to the dormant temperature of 4 °C. Turbot, which had been temporarily held for 24 hours and then cooled, were placed in the magnolia essential oil anesthesia tank. The turbot did not immediately enter an anesthetized state, but rather exhibited a series of behavioral characteristics as the anesthesia time increased. The anesthesia time of the turbot was observed and recorded through changes in these behavioral characteristics.

[0039] S4. Each turbot subjected to low-temperature dormancy was placed in a 35 cm × 50 cm packaging bag, and after being oxygenated, it was placed in a low-temperature incubator at 4°C to simulate waterless transportation. Its survival was observed every 12 hours, and the survival time was recorded and the survival rate was calculated.

[0040] S5. After transportation, remove the turbot and place it in clean water at 12-15 ℃. The turbot will fully recover.

[0041] Example 3

[0042] S1. Select fresh, live turbot weighing 750 ± 100 g and place them in pre-prepared seawater with a salinity of 28‰, pH 7.23, water temperature of 14 ± 1 ℃, and dissolved oxygen of 6.3 - 7.0 mg / L for 24 hours to eliminate stress reactions during transportation.

[0043] S2. After temporarily holding turbot for 24 hours, a gradient cooling treatment was carried out by adding ice packs. The cooling rate was controlled at 2-3℃ / h. For every 1℃ decrease in temperature, the respiratory rate and behavioral status of the turbot were observed and recorded. The temperature was lowered to 4℃.

[0044] S3. Magnolia oil at a concentration of 300 mg / L was prepared in an anesthesia tank according to the volume of seawater, and the seawater temperature was lowered to the dormant temperature of 4 ℃. Turbot, which had been temporarily held for 24 hours and then cooled, were placed in anesthesia tanks with different concentrations of magnolia oil. The turbot did not immediately enter an anesthetized state, but rather exhibited a series of behavioral characteristics as the anesthesia time increased. The anesthesia time of the turbot was observed and recorded through changes in these behavioral characteristics.

[0045] S4. Each turbot subjected to low-temperature dormancy was placed in a 35 cm × 50 cm packaging bag, and after being oxygenated, it was placed in a low-temperature incubator at 4°C to simulate waterless transportation. Its survival was observed every 12 hours, and the survival time was recorded and the survival rate was calculated.

[0046] S5. After transportation, remove the turbot and place it in clean water at 12-15 ℃. The turbot will fully recover.

[0047] Example 4

[0048] S1. Select fresh, live turbot weighing 750 ± 100 g and place them in pre-prepared seawater with a salinity of 28‰, pH 7.23, water temperature of 13-15℃, and dissolved oxygen of 6.3-7.0 mg / L. Temporarily hold them for 24 hours to eliminate stress reactions during transportation.

[0049] S2. After temporarily holding turbot for 24 hours, a gradient cooling treatment was carried out by adding ice packs. The cooling rate was controlled at 2-3℃ / h. For every 1℃ decrease in temperature, the respiratory rate and behavioral status of the turbot were observed and recorded. The temperature was lowered to 4℃.

[0050] S3. Prepare a 100 mg / L concentration of magnolia essential oil in an anesthesia tank according to the volume of seawater, and lower the seawater temperature to the dormancy temperature of 4℃. Place turbot that have been temporarily held for 24 hours and then cooled into anesthesia tanks with different concentrations of magnolia essential oil. The turbot will not immediately enter anesthesia, but will exhibit a series of behavioral characteristics as the anesthesia time prolongs.

[0051] S4. Then, an electric shock is performed with a voltage of 40 V, an output frequency of 25 Hz, and a shock duration of 3 seconds.

[0052] S5. Each turbot subjected to low-temperature dormancy was placed in a 35 cm × 50 cm packaging bag, and after being packaged and oxygenated, it was placed in a low-temperature incubator at 4 ℃ to simulate waterless transportation. Its survival was observed every 12 hours, and the survival time was recorded and the survival rate was calculated.

[0053] S6. After transportation, remove the turbot and place it in clean water at 12-15 ℃. The turbot will fully recover.

[0054] Example 5

[0055] S1. Select fresh, live turbot weighing 750 ± 100 g and place them in pre-prepared seawater with a salinity of 28‰, pH 7.23, water temperature of 13-15℃, and dissolved oxygen of 6.3-7.0 mg / L. Temporarily hold them for 24 hours to eliminate stress reactions during transportation.

[0056] S2. After temporarily holding turbot for 24 hours, a gradient cooling treatment was carried out by adding ice packs. The cooling rate was controlled at 2-3℃ / h. For every 1℃ decrease in temperature, the respiratory rate and behavioral status of the turbot were observed and recorded. The temperature was lowered to 4℃.

[0057] S3. Magnolia essential oil at a concentration of 200 mg / L was prepared in an anesthesia tank according to the volume of seawater, and the seawater temperature was lowered to the dormant temperature of 4℃. Turbot, which had been temporarily held for 24 hours and then cooled, were placed in anesthesia tanks with different concentrations of magnolia essential oil. The turbot did not immediately enter an anesthetized state, but rather exhibited a series of behavioral characteristics as the anesthesia time prolonged. The anesthesia time for the turbot was 10 minutes.

[0058] S4. Then, an electric shock is performed with a voltage of 30 V, an output frequency of 25 Hz, and a shock duration of 3 seconds.

[0059] S5. Each turbot subjected to low-temperature dormancy was placed in a 35 cm × 50 cm packaging bag, and after being packaged and oxygenated, it was placed in a low-temperature incubator at 4 ℃ to simulate waterless transportation. Its survival was observed every 12 hours, and the survival time was recorded and the survival rate was calculated.

[0060] S6. After transportation, remove the turbot and place it in clean water at 12-15 ℃. The turbot will fully recover.

[0061] Example 6

[0062] S1. Select fresh, live turbot weighing 750 ± 100 g and place them in pre-prepared seawater with a salinity of 28‰, pH 7.23, water temperature of 13-15℃, and dissolved oxygen of 6.3-7.0 mg / L. Temporarily hold them for 24 hours to eliminate stress reactions during transportation.

[0063] S2. After temporarily holding turbot for 24 hours, a gradient cooling treatment was carried out by adding ice packs. The cooling rate was controlled at 2-3℃ / h. For every 1℃ decrease in temperature, the respiratory rate and behavioral status of the turbot were observed and recorded. The temperature was lowered to 4℃.

[0064] S3. Magnolia essential oil at a concentration of 200 mg / L was prepared in an anesthesia tank according to the volume of seawater, and the seawater temperature was lowered to the dormant temperature of 4℃. Turbot, which had been temporarily held for 24 hours and then cooled, were placed in anesthesia tanks with different concentrations of magnolia essential oil. The turbot did not immediately enter an anesthetized state, but rather exhibited a series of behavioral characteristics as the anesthesia time prolonged. The anesthesia time for the turbot was 10 minutes.

[0065] S4. Then, an electric shock is performed with a voltage of 30 V, an output frequency of 15 Hz, and a shock duration of 3 seconds.

[0066] S5. Each turbot subjected to low-temperature dormancy was placed in a 35 cm × 50 cm packaging bag, and after being packaged and oxygenated, it was placed in a low-temperature incubator at 4 ℃ to simulate waterless transportation. Its survival was observed every 12 hours, and the survival time was recorded and the survival rate was calculated.

[0067] S6. After transportation, remove the turbot and place it in clean water at 12-15 ℃. The turbot will fully recover.

[0068] Example 7 (Control Example)

[0069] Comparison Example S1. Select fresh, live turbot weighing 750 ± 100 g and place them in pre-prepared seawater with a salinity of 28‰, pH 7.23, water temperature of 13-15℃, and dissolved oxygen of 6.3-7.0 mg / L for 24 hours to eliminate stress reactions during transportation.

[0070] S2. Lower the seawater temperature in the tank to the dormancy temperature of 4℃. Place the turbot that has been temporarily held for 24 hours and cooled into the low-temperature tank. As time goes on, the turbot's body temperature decreases and it exhibits a series of behavioral characteristics. Observe and record the turbot's respiratory rate, behavioral state, and low-temperature dormancy time.

[0071] S3. Each turbot subjected to low-temperature dormancy was placed in a 35 cm × 50 cm packaging bag, and after being oxygenated, it was placed in a low-temperature incubator at 4 ℃ to simulate waterless transportation. Its survival was observed every 12 h, and the survival time was recorded and the survival rate was calculated.

[0072] S4. After transportation, remove the turbot and place it in clean water at 12-15 ℃. The turbot will fully recover.

[0073] See the schematic diagram comparing the survival rates of turbot in Examples 1-6 and Example 7 above. Figure 1As shown in Figure 1, the survival rate of turbot after rehydration for 72 h and 24 h was 70% in the control example; the survival rate of turbot after rehydration for 72 h and 24 h in Example 1 reached 90%; and the survival rate of turbot after rehydration for 72 h and 24 h in Example 2 was 80%. Compared with the control example, this indicates that relatively low temperature, a certain amount of electric shock, or the addition of magnolia essential oil can improve the survival rate of turbot. The survival rates of turbot after rehydration for 72 h and 24 h in Example 3 were 95% and 90%, respectively, indicating that increasing the concentration of magnolia essential oil within a certain range improves the survival rate of turbot. The survival rate of turbot after 72 h in Example 4 reached 98% and 95%; and the survival rates of turbot after 72 h in Examples 5 and 6 reached 100% and 100%, respectively, with survival rates of 100% and 98% after rehydration, respectively. The results show that the simultaneous use of magnolia oil and electroshock combined with low-temperature transportation significantly increased the survival rate of turbot, with Example 5 showing the best results. Compared to Example 3, the concentration of magnolia oil used in Examples 4, 5, and 6 was reduced; compared to Example 1, the electroshock voltage in Examples 4, 5, and 6 was reduced, but the survival rate of turbot did not decrease but rather increased. This indicates that the simultaneous use of electroshock and magnolia oil can significantly reduce the concentration of magnolia oil and the electroshock voltage, while also improving the survival rate. During the research of this invention, it was also found that strictly adhering to a gradient cooling rate of 3℃ / h significantly improved the survival rate of turbot after rehydration.

[0074] Figure 2 This diagram illustrates the changes in cortisol levels in the blood of turbot in Examples 1-6 and the control example. Cortisol is a glucocorticoid produced under the regulation of the hypothalamic-pituitary-adrenal axis after the body is stimulated. It is transported throughout the body via the bloodstream, binds to receptors on the surface of tissue cells, and promotes glycogenolysis and gluconeogenesis, providing sufficient energy for the body to cope with stress. Figure 2 It can be seen that the cortisol concentration in the blood of turbot increased with the extension of the live transport time, and decreased after rehydration. The control example had the highest cortisol concentration at 48 and 72 hours of transport time, while the cortisol concentration in Example 1 was higher than in other examples. This indicates that electric shock caused a stronger stress response in turbot than that caused by magnolia oil or a combination of electric shock and magnolia oil. The cortisol content in Examples 4, 5, and 6 was significantly reduced, indicating that the combination of electric shock and magnolia oil with low-temperature transport can reduce the stress response in turbot.

[0075] Example 8

[0076] A method for keeping turbot alive without water, such as Figure 3 As shown, the method includes the following steps: S1. Temporary seawater holding: Turbot is temporarily held in seawater. S2. Cooling treatment: The turbot temporarily held in seawater is cooled to obtain cooled turbot. S3. Anesthesia treatment: The turbot subjected to the cooling treatment was anesthetized for 5-10 minutes in an anesthesia tank using white magnolia essential oil seawater solution. S4. Electroshock treatment: Electroshock treatment is performed on the turbot after anesthesia. S5. Packaging: After the turbot has been electrocuted, it is placed in a separate packaging bag, which is then filled with oxygen and transported in a low-temperature environment.

[0077] Furthermore, the salinity of the seawater described in S1 is 28‰.

[0078] Furthermore, the seawater has a pH of 7.23, a water temperature of 14±1℃, and a dissolved oxygen content of 6.3-7.0 mg / L.

[0079] Furthermore, the temporary holding time is 24 hours.

[0080] Furthermore, the cooling process described in S2 is a gradient cooling process, and the cooling rate of the gradient cooling is 3℃ / h.

[0081] Furthermore, the endpoint of the gradient cooling is 4-6℃.

[0082] Further, the anesthesia treatment described in S3 includes: preparing a 100 mg / L-500 mg / L concentration of magnolia essential oil seawater solution in an anesthesia tank, cooling the 100 mg / L magnolia essential oil seawater solution, and then placing the cooled turbot into the anesthesia tank.

[0083] Furthermore, the endpoint of the cooling of the magnolia essential oil seawater solution is 4-6℃.

[0084] Furthermore, the voltage of the electric shock in S4 is 30~60V, the frequency of the electric shock is 10-50Hz, and the duration of the electric shock is 3s.

[0085] Furthermore, the low-temperature environment described in S5 is 3℃-5℃.

[0086] The embodiments of the present invention have been described in detail above. Specific examples have been used to illustrate the principles and implementation methods of the present invention. The description of the above embodiments is only for the purpose of helping to understand the method and core ideas of the present invention. At the same time, for those skilled in the art, there will be changes in the specific implementation methods and application scope based on the ideas of the present invention. Therefore, the content of this specification should not be construed as a limitation of the present invention.

Claims

1. A method for preserving turbot in waterless conditions, characterized in that, The method includes the following steps: S1. Temporary seawater holding: Turbot is temporarily held in seawater. S2. Cooling treatment: The turbot temporarily held in seawater is cooled to obtain cooled turbot. S3. Anesthesia treatment: The turbot subjected to the cooling treatment was anesthetized for 5-10 minutes in an anesthesia tank using white magnolia essential oil seawater solution. S4. Electroshock treatment: Electroshock treatment is performed on the turbot after anesthesia. S5. Packaging: After the turbot has been electrocuted, it is placed in a separate packaging bag, which is then filled with oxygen and transported in a low-temperature environment.

2. The method according to claim 1, characterized in that, The salinity of the seawater described in S1 is 28‰.

3. The method according to claim 2, characterized in that, The seawater has a pH of 7.23, a water temperature of 14±1℃, and a dissolved oxygen content of 6.3-7.0 mg / L.

4. The method according to claim 3, characterized in that, The temporary holding period is 24 hours.

5. The method according to claim 1, characterized in that, The cooling process described in S2 is a gradient cooling process, and the cooling rate of the gradient cooling is 3℃ / h.

6. The method according to claim 5, characterized in that, The endpoint of the gradient cooling is 4-6℃.

7. The method according to claim 1, characterized in that, The anesthesia treatment described in S3 includes: preparing a 100 mg / L-500 mg / L concentration of magnolia essential oil seawater solution in an anesthesia tank, cooling the magnolia essential oil seawater solution, and then placing the cooled turbot into the anesthesia tank.

8. The method according to claim 7, characterized in that, The endpoint for cooling the white magnolia essential oil seawater solution is 4-6℃.

9. The method according to claim 1, characterized in that, The voltage of the electric shock in S4 is 30~60V, the frequency of the electric shock is 10-50Hz, and the duration of the electric shock is 3s.

10. The method according to claim 1, characterized in that, The low-temperature environment described in S5 is 3℃-5℃.

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