Use of a plant extract in a pet nutritional supplement

Abstract

The present application relates to the technical field of pet nutrition supplements, and particularly relates to an effect of a plant extract in a pet nutrition supplement, which comprises that the plant extract is a blood-replenishing and immunity traditional Chinese medicine extract, and application of the plant extract in preparation of a pet nutrition supplement for replenishing qi and blood and improving immune function; the blood-replenishing and immunity traditional Chinese medicine extract is obtained by water extraction or alcohol extraction from at least three kinds of raw materials in Fuling, Shanyao, poplar flower, Baizhu and Huoxiang, and the blood-replenishing effect is better than that of ferrous sulfate and the quality of red blood cells is also improved. The extract significantly reduces IL-6, and simultaneously reduces excessively increased IgG and IgM; the extract has a regulating effect on an immune hyperfunction state. The extract has high safety and no gastrointestinal stimulation.

Description

Technical Field

[0001] This invention relates to the field of pet nutritional supplement technology, and more particularly to the role of a plant extract in pet nutritional supplements. Background Technology

[0002] Pets (especially dogs and cats) often experience anemia, recovery from illness, or old age, leading to insufficient blood and qi and weakened immunity. Existing blood-tonifying products are mostly chemical iron supplements (such as ferrous sulfate), which, while rapidly increasing hemoglobin, can cause gastrointestinal irritation (vomiting, diarrhea, black stools) with long-term use, and iron overload can cause oxidative stress. Traditional Chinese medicine has a long history of tonifying qi and nourishing blood, but its application in pet nutritional supplements lacks systematic dose-response studies and experimental data. This invention, through animal experiments, is the first to demonstrate the precise efficacy of specific traditional Chinese medicine extracts and provides an optimal dosage range. Summary of the Invention

[0003] The purpose of this section is to outline some aspects of embodiments of the present invention and to briefly describe some preferred embodiments. Simplifications or omissions may be made in this section, as well as in the abstract and title of this application, to avoid obscuring the purpose of these documents; however, such simplifications or omissions should not be construed as limiting the scope of the invention.

[0004] In view of the problems with the existing effects of plant extracts in pet nutritional supplements, the present invention is proposed.

[0005] Therefore, the purpose of this invention is to provide a method for the use of plant extracts in pet nutritional supplements, which aims to overcome the side effects of chemical iron supplements and achieve bidirectional immune regulation.

[0006] To solve the above-mentioned technical problems, the present invention provides the following technical solution: the plant extract is a blood-nourishing and immune-boosting traditional Chinese medicine extract, and its application in the preparation of pet nutritional supplements for replenishing qi and blood and improving immune function; the blood-nourishing and immune-boosting traditional Chinese medicine extract is obtained by water extraction or alcohol extraction of at least three of the following raw materials: Poria cocos, Dioscorea opposita, Populus tomentosa, Atractylodes macrocephala, and Pogostemon cablin.

[0007] As a preferred embodiment of the role of the plant extract described in this invention in pet nutritional supplements, the weight ratio of the raw materials is Poria cocos: Dioscorea opposita: Populus tomentosa: Atractylodes macrocephala: Pogostemon cablin = (15-25):(15-25):(25-35):(10-20):(10-20).

[0008] As a preferred embodiment of the role of the plant extract described in this invention in pet nutritional supplements, the method of replenishing qi and nourishing blood manifests as increasing the red blood cell count, hemoglobin concentration, and / or hematocrit in anemic individuals.

[0009] As a preferred embodiment of the role of the plant extract described in this invention in pet nutritional supplements, the improvement of immune function is manifested in reducing the level of inflammatory factor IL-6 and / or reducing the level of immunoglobulins IgG and IgM.

[0010] As a preferred embodiment of the role of the plant extract described in this invention in pet nutritional supplements, the effective dosage of the blood-nourishing and immune-boosting traditional Chinese medicine extract is: 0.2 mL / kg / d for dogs, 0.15 mL / kg / d for cats, and 0.4 mL / kg / d for mice, based on the original extract.

[0011] As a preferred embodiment of the role of the plant extract described in this invention in pet nutritional supplements, the mass percentage of the blood-nourishing and immune-boosting traditional Chinese medicine extract in the pet nutritional supplement is 0.5% to 10%, preferably 1% to 5%, and more preferably 2% to 4%.

[0012] As a preferred embodiment of the role of the plant extract described in this invention in pet nutritional supplements, the pet nutritional supplement is an oral preparation, and the dosage form is selected from powder, tablet, capsule, paste, liquid dosage form, or added as an additive to pet staple food.

[0013] As a preferred embodiment of the role of the plant extract described in this invention in pet nutritional supplements, the pet is selected from dogs or cats.

[0014] As a preferred embodiment of the role of the plant extract described in this invention in pet nutritional supplements, the pet nutritional supplement is used in combination with chemical iron supplements to reduce the dosage of chemical iron supplements or alleviate their gastrointestinal side effects.

[0015] The beneficial effects of this invention are: superior blood-replenishing effect compared to ferrous sulfate, and improved erythrocyte quality. The extract significantly reduces IL-6, while also reducing excessively elevated IgG and IgM; it has a regulatory effect on immune hyperactivity. It is highly safe and causes no gastrointestinal irritation. Attached Figure Description

[0016] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort. Wherein: Figure 1 This is a schematic diagram of the RBC data experiment provided by the present invention.

[0017] Figure 2 This is a schematic diagram of the HCT data experiment provided by the present invention.

[0018] Figure 3 This is a schematic diagram of the HGB data experiment provided by the present invention.

[0019] Figure 4 This is a schematic diagram of the IL-6 data experiment provided by the present invention.

[0020] Figure 5 This is a schematic diagram of the IgG data experiment provided by the present invention.

[0021] Figure 6 This is a schematic diagram of the IgM data experiment provided by the present invention. Detailed Implementation

[0022] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0023] Example 1: Preparation of Blood-Nourishing and Immunizing Traditional Chinese Medicine Extracts - Water Extraction Process Take 200g of Poria cocos, 200g of Dioscorea opposita, 300g of Populus tomentosa flowers, 150g of Atractylodes macrocephala, and 150g of Pogostemon cablin. Grind them into powder and pass through a 40-mesh sieve. Add 10 times the volume (v / w) of purified water, soak for 30 minutes, decoct for 2 hours, and filter. Add 8 times the volume of water to the dregs and decoct for another hour. Combine the filtrates, concentrate under reduced pressure to a relative density of 1.10–1.15 (60℃), and spray dry (inlet air temperature 180℃, outlet air temperature 80℃) to obtain the extract powder, with a yield of approximately 18.5%. HPLC analysis showed that the pachymic acid content was ≥0.2%, and the total flavonoid content of Populus tomentosa flowers was ≥5.0%.

[0024] Example 2: Preparation of Blood-Nourishing and Immunizing Traditional Chinese Medicine Extracts - Alcohol Extraction Process Using the same raw materials as in Example 1, add 10 times the amount of 70% ethanol, reflux extract twice, 1 hour each time, combine the extracts, recover the ethanol under reduced pressure until no alcohol odor remains, concentrate to a relative density of 1.10-1.15 (60℃), vacuum dry (60℃, -0.08MPa), and pulverize to obtain extract powder, with a yield of approximately 15.2%. The total flavonoid content obtained by the ethanol extraction process is higher (≥8.0%), but the yield is lower.

[0025] Example 3: Pet Nutritional Supplements - Liquid Formulation Add 100g of the extract powder obtained in Example 1 to 800mL of purified water, stir to dissolve, then add 2g of potassium sorbate, 1g of citric acid, and 10mL of pet flavoring agent (chicken hydrolysate). Make up the purified water to 1000mL, filter, and fill into 10mL brown glass bottles, each containing 1g of extract. Recommended usage: For dogs and cats, administer one bottle orally daily for 5kg of body weight, for 14 consecutive days as one course of treatment.

[0026] Example 4: Pet Nutritional Supplements - Tablets 200g of the extract powder obtained in Example 1 was mixed with 150g of microcrystalline cellulose, 100g of lactose, 30g of crospovidone, and 5g of magnesium stearate, and compressed into 1000 tablets, each containing 200mg of the extract. The tablet disintegration time is ≤15 minutes. Recommended usage: 2 tablets orally per 5kg body weight for dogs and cats daily.

[0027] Comparative Example 1: Extract of Poria cocos alone Only 500g of Poria cocos was used, and the extract was prepared using the same water extraction process as in Example 1. Experiments showed that the RBC count in mice in the single-herb Poria cocos group was [missing value]. The levels were significantly lower than those of the compound group of this invention (15.1±0.47), demonstrating the synergistic effect of the compound.

[0028] Comparative Example 2: Flavorless Formula - Without Poplar Flower 250g of Poria cocos, 250g of Dioscorea opposita, 200g of Atractylodes macrocephala, and 200g of Pogostemon cablin were used to prepare an extract using the same water extraction process as in Example 1. Experiments showed that the IL-6 level in the taste-deficient group of mice decreased to 48.3±4.2 pg / mL, while the level in the compound group of this invention decreased to 37.4±5.2 pg / mL, demonstrating that poplar flowers significantly contribute to reducing IL-6.

[0029] Experimental Example: Validation of the efficacy of anemic mouse model Experimental materials: C57BL / 6J mice, male, 4 weeks old, weighing 18-22g. Low-iron diet (iron content <5 mg / kg) was purchased from Suzhou Modern Animal Feed Co., Ltd.

[0030] Modeling method: After one week of acclimatization, except for the normal control group, all mice in the other groups were fed a low-iron diet. 0.2 mL of blood was excised from the orbital region of each mouse weekly for four consecutive weeks. Hemoglobin (HGB) was measured in the fourth week; successful modeling was indicated when HGB was below 100 g / L.

[0031] Animal grouping and drug administration: After successful modeling, animals were randomly divided into 6 groups, with 6 animals in each group: Normal control group: Normal feed + purified water by gavage; Model control group: low-iron diet + purified water by gavage; Ferrous sulfate group: low-iron feed + ferrous sulfate 2 mg / kg by gavage; Low-dose extract group: low-iron feed + 0.2 mL / kg of blood-enriching and immune-boosting traditional Chinese medicine extract administered by gavage; Medium-dose group of extract: low-iron feed + 0.4 mL / kg of blood-enriching and immune-boosting traditional Chinese medicine extract administered by gavage; High-dose extract group: low-iron feed + 0.8 mL / kg of blood-enriching and immune-boosting traditional Chinese medicine extract administered by gavage; The gavage volume was 20 mL / kg, and the gavage was administered continuously for 28 days.

[0032] Detection indicators: At the experimental endpoint, blood was collected, and red blood cell count (RBC), hemoglobin level (HGB), and hematocrit (HCT) were measured using an animal blood analyzer. Serum was collected, and IgG, IgM, and IL-6 levels were measured according to the ELISA kit instructions.

[0033] Statistical results are expressed as mean ± SD. Statistical analysis was performed using SPSS software, and p < 0.05 indicated that the difference was statistically significant.

[0034] Regarding the effects of the samples on routine blood count indicators, under the conditions of this experiment, the blood-nourishing and immune-boosting traditional Chinese medicine extract significantly increased the levels of RBC, HGB, and HCT in anemic mice, as detailed in Table 1. Figures 1-3 Compared with the model control group, p < 0.01, p < 0.001.

[0035] Table 1. Blood routine indicators (n=6) Regarding the effects of the samples on inflammatory markers, under the experimental conditions, the blood-nourishing and immune-boosting traditional Chinese medicine extract significantly reduced the levels of IL-6, IgG, and IgM in anemic mice. See Table 2 for details. Figures 4-6 .

[0036] Table 2. Inflammatory markers (n = 6) Compared with the model control group, p < 0.05, p < 0.01, p < 0.001.

[0037] Compared with the model control group, the medium dose group of the extract showed an increase of 63% in RBC (p<0.001), 95% in HGB (p<0.001), and 92% in HCT (p<0.001).

[0038] Compared with the ferrous sulfate group, the medium dose group of the extract had significantly higher RBC (15.1 vs 12.0, p<0.01), while there was no significant difference in HGB (173 vs 178, p>0.05).

[0039] Compared with the model control group, the medium-dose extract group showed a 39% decrease in IL-6 (p<0.001), a 40% decrease in IgG (p<0.01), and a 38% decrease in IgM (p<0.01). Under the experimental conditions, the blood-tonifying and immune-enhancing traditional Chinese medicine extract demonstrated the effects of tonifying qi and blood and improving immunity, specifically by significantly increasing the levels of RBC, HGB, and HCT in anemic mice and significantly decreasing the levels of IL-6, IgG, and IgM in anemic mice.

[0040] The above provides a detailed description of the role of a plant extract in pet nutritional supplements proposed in this invention, and elucidates the principles and implementation methods of this invention. The above description of the embodiments is only for the purpose of helping to understand the method and core ideas of this invention; at the same time, for those skilled in the art, there will be changes in the specific implementation methods and application scope based on the ideas of this invention. Therefore, the content of this specification should not be construed as a limitation of this invention.

Claims

1. The effect of a plant extract in pet nutritional supplements, characterized in that, The plant extract is a traditional Chinese medicine extract for replenishing blood and immunity, and is used in the preparation of pet nutritional supplements for replenishing qi and blood and improving immune function; the traditional Chinese medicine extract for replenishing blood and immunity is obtained by water extraction or alcohol extraction of at least three of the following raw materials: Poria cocos, Dioscorea opposita, Populus tomentosa, Atractylodes macrocephala, and Pogostemon cablin.

2. The effect of the plant extract in pet nutritional supplements according to claim 1, characterized in that: The weight ratio of the raw materials is Poria cocos: Dioscorea opposita: Populus tomentosa: Atractylodes macrocephala: Pogostemon cablin = (15-25):(15-25):(25-35):(10-20):(10-20).

3. The effect of the plant extract according to claim 1 in pet nutritional supplements, characterized in that: The aforementioned replenishing of qi and nourishing of blood manifests as increasing the red blood cell count, hemoglobin concentration, and / or hematocrit in individuals with anemia.

4. The effect of the plant extract according to claim 1 in pet nutritional supplements, characterized in that: The improvement in immune function is manifested in reducing the level of the inflammatory factor IL-6 and / or reducing the levels of immunoglobulins IgG and IgM.

5. The effect of the plant extract according to claim 1 in pet nutritional supplements, characterized in that: The effective dosage of the blood-nourishing and immune-boosting traditional Chinese medicine extract is as follows: 0.2 mL / kg / d for dogs, 0.15 mL / kg / d for cats, and 0.4 mL / kg / d for mice, based on the original extract.

6. The effect of the plant extract according to claim 5 in pet nutritional supplements, characterized in that: The blood-nourishing and immune-boosting herbal extract in the pet nutritional supplement has a mass percentage of 0.5% to 10%, preferably 1% to 5%, and more preferably 2% to 4%.

7. The effect of the plant extract according to claim 6 in pet nutritional supplements, characterized in that: The pet nutritional supplement is an oral preparation, and the dosage form is selected from powder, tablet, capsule, paste, liquid dosage form, or as an additive to pet staple food.

8. The effect of the plant extract according to claim 1 in pet nutritional supplements, characterized in that: The pet is selected from dogs or cats.

9. The effect of the plant extract according to claim 1 in pet nutritional supplements, characterized in that: The pet nutritional supplement is used in combination with chemical iron supplements to reduce the dosage of chemical iron supplements or to alleviate their gastrointestinal side effects.

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