Sheep feed additive, sheep milk and methods and uses thereof

Abstract

The application discloses a sheep feed additive, sheep milk and a preparation method and application thereof. The sheep feed additive is prepared from the following components in parts by weight: 100-500 parts by weight of green wheat and 0.3-2 parts by weight of curcumin; wherein the amount of the green wheat is based on the dry matter contained in the green wheat; and the green wheat is the whole plant wheat in the budding stage to the initial stage of grain filling. The sheep feed additive can significantly increase the content of anti-inflammatory active substances in the sheep milk.

Description

Technical Field

[0001] This invention relates to a sheep feed additive, sheep milk, its preparation method, and its uses. Background Technology

[0002] Chronic low-grade inflammation is a common pathological basis for many metabolic diseases, including obesity, type 2 diabetes, cardiovascular disease, and non-alcoholic fatty liver disease. At the molecular level, this inflammatory state is typically characterized by elevated levels of circulating pro-inflammatory cytokines (such as interleukin-6 and tumor necrosis factor-α) and decreased levels of anti-inflammatory factors (such as adiponectin), creating an inflammatory microenvironment unfavorable to the body's health. Therefore, intervening in and regulating the body's inflammation levels through dietary nutrition has become an important direction in modern functional food research and development.

[0003] Currently, foods and dietary supplements claiming anti-inflammatory properties on the market mainly fall into three categories: First, extracts of active ingredients derived from plants, such as resveratrol and Omega-3 polyunsaturated fatty acids. Second, probiotics, prebiotics, and synbiotics, which exert indirect anti-inflammatory effects by regulating the gut microbiota. Third, certain vitamins and minerals. While these products have gained some market recognition, they also have limitations. For example, plant extracts may have low bioavailability and unpleasant taste; probiotic products have significant strain specificity in their efficacy, and their stability is greatly affected by storage and processing conditions; while chemically synthesized supplements are not as advantageous in terms of consumer perception of "natural and safe."

[0004] Goat milk, as a traditional natural food, has widely recognized nutritional value. For example, goat milk has smaller fat globules, a superior protein composition, is more easily digested and absorbed, and is rich in various bioactive substances. Goat milk components have the potential to regulate immunity and improve gut health. However, current technologies for exploring the anti-inflammatory effects of goat milk are significantly insufficient: First, most studies remain at the stage of nutritional component analysis or in vitro experiments, lacking systematic, especially in vivo efficacy evaluations with clearly defined endpoints for key indicators of inflammatory pathways (such as IL-6 and TNF-α). Second, the content of anti-inflammatory active ingredients in ordinary goat milk is limited, and its efficacy is not significant or stable enough. Finally, how to target and increase the anti-inflammatory active substances in goat milk through upstream farming processes (such as feed nutrition regulation) to achieve the industrialization technology of producing "functional milk" from the source remains a blank.

[0005] CN105265796A discloses a feed for Hu sheep ewes that can improve the immunity of lambs. The feed is made from the following components in parts by weight: 30 parts corn, 40 parts soybean meal, 50 parts soybean straw, 2 parts cottonseed cake, 2 parts hawthorn powder, 0.2 parts selenium-enriched yeast, 0.2 parts curcumin, 0.3 parts wheat oligopeptides, 1 part salt, 0.2 parts sweet peptides, 0.2 parts xylanase, 3 parts immunity enhancer, 5 parts roasted soybean powder, and 2 parts talc. This feed can meet the growth and development needs of the fetus during pregnancy in Hu sheep ewes and can improve the disease resistance of lambs. However, the feed has a complex composition, and the immunity enhancer is made from 21 kinds of traditional Chinese medicine, which significantly reduces the palatability of the feed. Furthermore, this feed does not address the anti-inflammatory properties of sheep milk.

[0006] CN107080066A discloses a feed suitable for lactating ewes. This feed comprises 8-15 parts by weight of corn, 8-15 parts by weight of wheat, 10-18 parts by weight of alfalfa, 10-18 parts by weight of white clover, 15-20 parts by weight of wheat germ meal, 15-20 parts by weight of feather meal, 15-20 parts by weight of soybean meal, 15-20 parts by weight of sesame meal, 2-8 parts by weight of bone meal, 2-8 parts by weight of limestone powder, 4-10 parts by weight of curcumin, 4-10 parts by weight of yucca extract, 3-8 parts by weight of vitamins, 5-10 parts by weight of motherwort, 5-10 parts by weight of cyperus rotundus, 5-10 parts by weight of pulsatilla chinensis, and 5-10 parts by weight of sophora flavescens. This feed also uses an excessive amount of traditional Chinese medicine preparations, which may reduce its palatability. Furthermore, the milk produced by ewes fed this feed only improves the growth performance of lambs and does not involve anti-inflammatory properties. Summary of the Invention

[0007] In view of the above, one object of the present invention is to provide a sheep feed additive and a method for preparing the same, which can significantly increase the anti-inflammatory active substances in sheep milk. Another object of the present invention is to provide the use of the above-mentioned sheep feed additive. A further object of the present invention is to provide sheep milk and a method for preparing the same. Yet another object of the present invention is to provide the use of the above-mentioned sheep milk.

[0008] The present invention achieves the above objectives using the following technical solutions.

[0009] On one hand, the present invention provides a sheep feed additive, which is prepared from the following ingredients in parts by weight: 100-500 parts by weight of green wheat and 0.3-2 parts by weight of curcumin; wherein the amount of green wheat is based on its dry matter content; wherein the green wheat is whole wheat from the heading stage to the early grain-filling stage.

[0010] The present invention also provides a method for preparing the above-mentioned sheep feed additive, including the step of mixing green wheat and curcumin.

[0011] On the other hand, the present invention also provides the use of the above-mentioned sheep feed additive in increasing the content of anti-inflammatory active substances in sheep milk.

[0012] In another aspect, the present invention also provides a method for preparing goat milk, comprising the following steps: mixing the above-mentioned goat feed additive with a basic diet and feeding it to a lactating goat, the milk produced by the goat being goat milk.

[0013] According to the preparation method of the present invention, preferably, the weight ratio of the above-mentioned sheep feed additive to the basal diet is 1:5 to 20.

[0014] According to the preparation method of the present invention, preferably, the feeding period is at least 30 days.

[0015] The present invention also provides goat milk prepared by any of the above preparation methods.

[0016] In another aspect, the present invention also provides the use of the above-mentioned goat milk in the preparation of anti-inflammatory foods.

[0017] According to the intended use described in this invention, preferably, the anti-inflammatory food is a dairy product prepared from the aforementioned goat milk.

[0018] According to the intended use of the present invention, preferably, the dairy product is selected from at least one of fresh milk, fermented yogurt, milk powder, and dairy-containing complete nutritional formula foods.

[0019] The sheep feed additive of this invention achieves a synergistic effect between green fodder and functional additives, significantly increasing the content of anti-inflammatory active substances in sheep milk. The sheep milk of this invention exhibits a significant anti-inflammatory effect. Detailed Implementation

[0021] The present invention will be further described below with reference to specific embodiments, but the scope of protection of the present invention is not limited thereto.

[0022] <Sheep Feed Additives and Their Preparation Methods> This invention provides a sheep feed additive, which is made from ingredients including green wheat and curcumin.

[0023] According to one embodiment of the present invention, the amount of green wheat used, based on the dry matter content, can be 100 to 500 parts by weight, preferably 120 to 400 parts by weight, and more preferably 150 to 300 parts by weight.

[0024] In this invention, green wheat refers to the whole-plant wheat from the heading stage to the early grain-filling stage. The heading stage and early grain-filling stage are well-known concepts in the art. The heading stage of wheat refers to the period when more than 50% of the wheat ears in the field emerge from the leaf sheath at half their length; this generally lasts 5-7 days. The early grain-filling stage of wheat refers to the period 1-10 days after flowering when more than 50% of the grains in the field reach 75-80% of their final grain length; this generally lasts 10-15 days. The dry matter content of the green wheat used in this invention during this period can be 20-45 wt%, preferably 25-40 wt%, and more preferably 30-40 wt%.

[0025] According to one embodiment of the present invention, the amount of curcumin can be 0.3 to 2 parts by weight, preferably 0.4 to 1.5 parts by weight, and more preferably 0.5 to 1 part by weight.

[0026] According to one embodiment of the present invention, the sheep feed additive is prepared from the following components in parts by weight: Based on the dry matter content, it contains 100 to 500 parts by weight of green wheat and 0.3 to 2 parts by weight of curcumin.

[0027] According to a preferred embodiment of the present invention, the sheep feed additive is prepared from an ingredient comprising the following parts by weight: Based on the dry matter content, it contains 120–400 parts by weight of green wheat and 0.4–1.5 parts by weight of curcumin.

[0028] According to another preferred embodiment of the present invention, the sheep feed additive is prepared from an ingredient comprising the following parts by weight: Based on the dry matter content, it contains 150–300 parts by weight of green wheat and 0.5–1 parts by weight of curcumin.

[0029] According to a specific embodiment of the present invention, the sheep feed additive is prepared from only the following components in parts by weight: Based on the dry matter content, it contains 100 to 500 parts by weight of green wheat and 0.3 to 2 parts by weight of curcumin.

[0030] In this invention, curcumin can be a commercially available product or it can be extracted and prepared from plants of the genus Curcuma using methods known in the art.

[0031] This invention discovers that combining green wheat from the heading stage to the early grain-filling stage with curcumin to form a sheep additive can achieve a synergistic effect between green fodder and functional additives. Feeding these two additives to lactating sheep can increase the content of anti-inflammatory active substances in sheep milk and reduce the content of exogenous chemicals in sheep milk. At the same time, it can also reduce the metabolic burden on sheep.

[0032] In this invention, limiting the composition and dosage of sheep feed additives to the above-mentioned range is beneficial to increasing the content of 2,4-dihydroxyquinoline and indole-3-carboxylic acid-O-sulfate in sheep milk, and beneficial to reducing the content of glycochenodeoxycholic acid, sulfate ester and proflavin in sheep milk.

[0033] The present invention also provides a method for preparing the above-mentioned sheep feed additive, including the step of mixing green wheat and curcumin.

[0034] In this invention, mixing can be performed using any mixing method and mixing equipment known in the art, which will not be described in detail here.

[0035] <Uses of Sheep Feed Additives> The present invention also provides the use of the above-mentioned sheep feed additive, especially the use of the above-mentioned sheep feed additive in increasing the content of anti-inflammatory active substances in sheep milk.

[0036] According to one embodiment of the present invention, the anti-inflammatory active substance may be at least one selected from 2,4-dihydroxyquinoline and indole-3-carboxylic acid-O-sulfate, preferably 2,4-dihydroxyquinoline and indole-3-carboxylic acid-O-sulfate. According to a preferred embodiment of the present invention, the above-mentioned sheep feed additive can increase the content of 2,4-dihydroxyquinoline in sheep milk by more than 25%, preferably more than 27%, and more preferably more than 29%. The above-mentioned sheep feed additive can increase the content of indole-3-carboxylic acid-O-sulfate in sheep milk by more than 20%, preferably more than 22%, and more preferably more than 23%.

[0037] According to one embodiment of the present invention, the above-mentioned sheep feed additive can also reduce the content of glycosaminoglycans in sheep milk by more than 45%, preferably more than 46.5%, more preferably more than 48%; reduce the content of sulfate esters in sheep milk by more than 30%, preferably more than 31%, more preferably more than 32%; and reduce the content of proflavin in sheep milk by more than 65%, preferably more than 66%, more preferably more than 68%.

[0038] In this invention, 2,4-dihydroxyquinoline, as a quinoline-based natural product, possesses antioxidant and anti-inflammatory activities. Indole-3-carboxylic acid-O-sulfate, a microbial metabolite of tryptophan, can act as an agonist of the aryl hydrocarbon receptor (AHR) and participate in regulating the expression of inflammatory factors such as IL-6. The upregulation of these two substances indicates that the sheep feed additive of this invention can significantly increase the content of anti-inflammatory active substances in sheep milk.

[0039] Glycine-chenodeoxycholic acid is associated with lipid metabolism. Sulfate esters are metabolites of proanthocyanidins and belong to the gut microbiota metabolic products. Downregulation of these two substances indicates that the sheep feed additive can significantly reduce the lipid metabolic load in sheep milk, thus optimizing the metabolic profile. Proflavin is an exogenous DNA intercalating agent; downregulation of its content indicates that the sheep feed additive of this invention can significantly reduce the non-specific exogenous chemical load in sheep milk.

[0040] <Goat Milk and its Preparation Methods> The present invention also provides a method for preparing goat milk.

[0041] According to one embodiment of the present invention, a method for preparing goat milk includes the following steps: mixing the above-mentioned goat feed additive with a basic diet and feeding it to a lactating goat, the milk produced by the goat is goat milk.

[0042] According to one embodiment of the present invention, the weight ratio of sheep feed additive to basal diet can be 1:5 to 20, preferably 1:8 to 17, and more preferably 1:10 to 15.

[0043] According to one embodiment of the present invention, the feeding period may be at least 30 days, preferably at least 45 days, more preferably at least 50 days, and even more preferably until the end of lactation.

[0044] A reasonable feeding ratio and number of days for sheep feed additives can ensure that the nutrients and curcumin in green wheat are fully absorbed by the sheep and avoid waste. This is conducive to maximizing the synergistic effect of green wheat and curcumin, and significantly increasing the content of anti-inflammatory substances in sheep milk.

[0045] In this invention, the raw materials for sheep feed additives can be first mixed to prepare the feed additive, and then mixed with the basal diet for feeding sheep. Alternatively, the raw materials for sheep feed additives can be directly mixed with the basal diet for feeding sheep. The basal diet of this invention can be any sheep basal diet known in the art, and is not particularly limited here. For example, it can be a TMR diet (total mixed ration) or a mixture of TMR diet and concentrate supplement.

[0046] In this invention, the sheep can be selected from at least one of the subfamily Caprinae of the Bovidae family, preferably dairy cattle or dairy sheep, including but not limited to Saanen sheep, Tugenburg sheep, Guanzhong sheep, Laoshan goat, East Frisen sheep, Hu sheep, etc.

[0047] The present invention also provides goat milk prepared by any of the above preparation methods.

[0048] <Uses of Goat Milk> The present invention also provides the use of the above-mentioned goat milk in the preparation of anti-inflammatory foods.

[0049] According to one embodiment of the present invention, goat milk can reduce the concentration of TNF-α in the serum of an organism after surgery by more than 37%, preferably more than 38%, and more preferably more than 38.5%. The concentration of IL-6 in the serum can be reduced by more than 40%, preferably more than 41.5%, and more preferably more than 42%. The concentration of IL-1β can be reduced by more than 20%, preferably more than 22%, and more preferably more than 25%. The organism involved in this invention can be humans or various other mammals.

[0050] According to one embodiment of the present invention, the anti-inflammatory food can be a dairy product prepared using the aforementioned goat milk as a raw material. According to a preferred embodiment of the present invention, the dairy product can be selected from at least one of fresh milk, fermented yogurt, milk powder, and dairy-containing complete nutritional formula food, preferably at least one of fresh milk, fermented yogurt, and milk powder, and more preferably fresh milk, fermented yogurt, or milk powder.

[0051] <Test Method> Metabolite determination: Non-targeted metabolomics analysis was performed using liquid chromatography-mass spectrometry (LC-MS). LC was performed using a Thermo Fisher Orbitrap Exploris 120 system. Mass spectrometry was performed using a Thermo Fisher Vanquish system. Chromatographic conditions: An ACQUITY UPLC BEH C18 column (2.1 mm × 100 mm, 1.7 μm) was used. Mobile phase A was 0.1% vol formic acid aqueous solution, and mobile phase B was 0.1% vol formic acid acetonitrile solution, with gradient elution. Mass spectrometry was performed using electrospray ionization (ESI) in both positive and negative ion modes.

[0052] Serum inflammatory factors were measured using an ELISA kit manufactured by Wuhan Feien Biotechnology Co., Ltd.

[0053] <Raw Material Description> Unless otherwise specified, all raw materials used in the following examples are commercially available products.

[0054] Curcumin was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd., with product code C400271.

[0055] Example 1 Preparation of sheep feed additives: Sheep feed additive was prepared by mixing 200 parts by weight of green wheat (35 wt% dry matter content) harvested 30 days after heading and 0.8 parts by weight of curcumin using a plow-type mixer.

[0056] Experimental Example 1 1. Animal experiments and metabolomics analysis 1.1 Animal Experiments Twenty Saanen dairy goats, aged 30–40 days postpartum and in lactation, with similar weights and good health, were randomly divided into two groups (n=10 in each group). The treatment methods for each group are as follows: Control group: fed a standard basal diet (mainly TMR, supplemented with concentrate, 2.5 kg dry matter intake per head per day). Detailed formulation is as follows: The experiment used a total mixed ration (TMR) feeding model. The TMR formula mainly consisted of alfalfa hay (15.5 wt%), oat hay (14.5 wt%), beet pulp pellets (16.0 wt%), and flaked corn (6.5 wt%), with added functional components such as Jianmeibao (5.0 wt%, purchased from Shandong Rongbao Biotechnology Co., Ltd.), Nenage D (2.0 wt%, purchased from Shandong Provincial Feed Industry Co., Ltd.), and sodium bicarbonate (0.5 wt%). After adjustment, the TMR had a dry matter content of 94.0 wt%, crude protein (CP) of 14.5 wt%, and neutral detergent fiber (NDF) of 51.5 wt%. The concentrate supplement mainly consists of wheat bran (40.0 wt%), soybean meal (25.0 wt%), and corn flour (20.0 wt%), with a dry matter content of 89.0 wt%, crude protein (CP) content of 19.5 wt%, crude fat content of 5.5%, crude fat (EE) content of 4.25 wt%, crude ash content of 9.54 wt%, neutral detergent fiber (NDF) content of 41.69 wt%, and acid detergent fiber (ADF) content of 23.60 wt%.

[0057] Experimental group: In addition to the above-mentioned conventional basic diet, the sheep feed additive of Example 1 was added at 200.8g / day / head (200g / day / head of green wheat and 0.8g / day / head of curcumin based on dry matter content).

[0058] The trial lasted for 60 days (10 days of acclimatization and 50 days of formal trial). After the trial, fresh goat milk samples were collected from each group of dairy goats before morning feeding and stored at -80℃ for later use.

[0059] 1.2 Metabolomics Analysis Metabolites in fresh goat milk samples from each group were analyzed by LC-MS. After peak alignment and normalization, the collected data underwent principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA), with VIP>1 and... PA value <0.05 was used to screen differentially expressed metabolites, where VIP stands for Variable Importance for the Projection. For the identified key differentially expressed metabolites, targeted quantification was performed using standards.

[0060] 2. Experimental Results The differential metabolites and their abundance in fresh goat milk from the experimental group and the control group were analyzed and compared, as shown in Table 1 below.

[0061] Table 1 As shown in Table 1, compared with the control group, the content of 2,4-dihydroxyquinoline and indole-3-carboxylic acid-O-sulfate, which have antioxidant and anti-inflammatory activities, was significantly increased in the goat milk of the experimental group. P <0.05), representing increases of 29% and 23% respectively.

[0062] Meanwhile, the levels of glycocholic acid and sulfate esters (a metabolite of proanthocyanidins) in the goat milk of the experimental group were significantly reduced, indicating a lower lipid metabolic load and a more optimized metabolic profile in the goat milk of the experimental group. Furthermore, the level of proflavin, an exogenous DNA intercalation agent, in the goat milk of the experimental group was significantly reduced (to only 32% of that in the control group). P <0.05 indicates that the non-specific exogenous chemical load in the goat milk of the experimental group was significantly reduced.

[0063] The above results indicate that the sheep feed additive of the present invention significantly enhances the anti-inflammatory function of sheep milk through a dual pathway of increasing the content of anti-inflammatory active ingredients in sheep milk and reducing adverse metabolic and exogenous loads in sheep milk.

[0064] Application Experiment Example 1 1. Animal experiments and serum inflammatory factor assays 1.1 Animal Experiments Eight-week-old SPF-grade C57BL / 6J mice (weighing 18–22 g) were randomly divided into four groups of eight mice each, as follows (n=8): Sham group: Administered normal saline by gavage (0.2 mL / day), with abdominal opening and closure performed only, without cecal ligation and perforation.

[0065] Model control group (CLP group): Administered normal saline (0.2 mL / day) by gavage and underwent cecal ligation and perforation (CLP) to induce postoperative systemic inflammation.

[0066] Goat milk control group (MC group): Goat milk produced by the control group in gavage experiment 1 (0.2 mL / day) was used for cecal ligation and perforation.

[0067] Goat milk experimental group (MG group): Goat milk produced by the experimental group in gavage experiment 1 (0.2 mL / day) was used for cecal ligation and perforation.

[0068] Preoperative gavage was administered to mice for 7 days. On the day of surgery, each group of mice underwent a final gavage 1 hour before surgery. After anesthesia with isoflurane, a 1 cm incision was made along the midline of the abdomen to expose the cecum. Except for the Sham group, the other three groups had the cecum ligated at the distal third with 4-0 silk sutures. An 18G needle was used to puncture the ligated cecum twice, a small amount of intestinal contents were squeezed out, and the cecum was returned to its original position. The abdomen was then closed layer by layer. In the Sham group, only an open abdomen was performed followed by closure; no cecal ligation or perforation was performed. Postoperatively, mice had free access to water and were fasted for 12 hours but allowed free access to water before resuming a normal diet.

[0069] 1.2 Serum inflammatory factor measurement Blood samples were collected from mice in each group 24 hours post-surgery using the ocular blood sampling method. The blood samples were allowed to stand at room temperature for 35 minutes, followed by centrifugation at 3000 rpm for 10 minutes. The supernatant serum was separated and stored at -20°C for later analysis. Serum levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) were detected using an enzyme-linked immunosorbent assay (ELISA) kit. The specific procedures were performed according to the kit instructions.

[0070] Statistical analysis was performed using SPSS 22.0 software for one-way ANOVA. Tukey's HSD test was used for comparisons between groups. Data are expressed as mean ± standard deviation (SD). P <0.05 indicates a statistically significant difference.

[0071] 2. Experimental Results The serum inflammatory factors were measured, as shown in Table 2 below.

[0072] Table 2 Note: Different letters in the same column indicate significant differences. P <0.05); the units for TNF-α, IL-6, and IL-1β are all pg / mL.

[0073] As shown in Table 2, compared with the sham-operated group (Sham group), the serum levels of TNF-α, IL-6, and IL-1β in the model control group (CLP group) were significantly increased. P<0.05). Among them, the IL-6 level increased sharply from 18.48 pg / mL in the Sham group to 251.30 pg / mL in the CLP group (an increase of about 13.6 times), and the TNF-α level increased from 25.49 pg / mL to 69.11 pg / mL (an increase of about 2.7 times), indicating that cecal ligation and perforation (CLP) successfully induced a systemic inflammatory response in mice after surgery.

[0074] The experimental results after intervention with goat milk are shown below: Compared with the CLP group, the goat milk control group (MC group) only significantly reduced IL-6 levels (from 251.30 pg / mL to 201.90 pg / mL). P <0.05, but had no significant effect on TNF-α and IL-1β levels. Specifically, the TNF-α level in the MC group (66.28 pg / mL) was not statistically different from that in the CLP group (69.11 pg / mL). P >0.05). The IL-1β level (54.86 pg / mL) was not significantly different from that in the CLP group (59.80 pg / mL). P >0.05).

[0075] Compared with the CLP group, the goat milk experimental group (MG group) showed a significant inhibitory effect on all three inflammatory factors: TNF-α levels decreased from 69.11 pg / mL to 42.45 pg / mL. P <0.05%, a decrease of approximately 38.6%. IL-6 levels decreased from 251.30 pg / mL to 145.64 pg / mL ( P <0.05%, a decrease of approximately 42.0%. IL-1β levels decreased from 59.80 pg / mL to 44.55 pg / mL ( P <0.05), a decrease of approximately 25.5%.

[0076] The comparison between the MG group and the MC group is as follows: Compared with the MC group, the TNF-α level (42.45 pg / mL vs 66.28 pg / mL) and IL-6 level (145.64 pg / mL vs 201.90 pg / mL) in the MG group were significantly reduced (P<0.05), with reductions of 35.9% and 27.9%, respectively. In particular, the MC group had no significant inhibitory effect on TNF-α, while the MG group significantly reduced TNF-α levels, indicating that the anti-inflammatory effect of the goat milk of the present invention is significantly better than that of the control group goat milk.

[0077] In summary, goat milk produced using the goat feed additive of this invention has a significant inhibitory effect on postoperative systemic inflammation induced by abdominal surgery, and can simultaneously reduce the levels of three core pro-inflammatory factors: TNF-α, IL-6, and IL-1β. Its anti-inflammatory effect is significantly superior to that of ordinary goat milk. These results indicate that the goat milk of this invention has potential application value in postoperative patient rehabilitation and perioperative nutritional support, and can serve as a high-quality raw material for developing anti-inflammatory functional dairy products and postoperative medical foods.

[0078] This invention is not limited to the above-described embodiments. Any modifications, improvements, or substitutions that can be conceived by those skilled in the art without departing from the essential content of this invention fall within the scope of this invention.

Claims

1. A sheep feed additive, characterized in that, It is prepared from the following components in parts by weight: 100-500 parts by weight of green wheat and 0.3-2 parts by weight of curcumin; wherein the amount of green wheat is based on its dry matter content; the green wheat is whole wheat from the heading stage to the early grain-filling stage.

2. A method for preparing a sheep feed additive according to claim 1, characterized in that, This includes the step of mixing green wheat and curcumin.

3. The use of the sheep feed additive according to claim 1 in increasing the content of anti-inflammatory active substances in sheep milk.

4. A method for preparing goat milk, characterized in that, The process includes the following steps: mixing the sheep feed additive of claim 1 with the basal diet and feeding it to a lactating sheep, the milk produced by the sheep being sheep milk.

5. The preparation method according to claim 4, characterized in that, The weight ratio of the sheep feed additive to the basal diet is 1:5 to 20.

6. The preparation method according to claim 4, characterized in that, The feeding period is at least 30 days.

7. Goat milk prepared by any one of the preparation methods according to claims 4 to 6.

8. The use of goat milk according to claim 7 in the preparation of anti-inflammatory foods.

9. The use according to claim 8, characterized in that, The anti-inflammatory food is a dairy product prepared using goat milk as the raw material as described in claim 7.

10. The use according to claim 9, characterized in that, The dairy products are selected from at least one of fresh milk, fermented yogurt, milk powder, and dairy-containing complete nutritional formula foods.

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