Compositions for preventing hypertrophic cardiomyopathy in cats and methods of making the same

Abstract

The present application relates to the technical field of pet food, and discloses a composition for preventing feline hypertrophic cardiomyopathy and a preparation method thereof.The composition comprises, in percentage by mass, 30-50% of an animal protein source, 10-20% of an animal fat source, 3-8% of dietary fiber, 5-15% of a starch substance, 1-5% of a functional nutrition component, 1-3% of a traditional Chinese medicine component, and the rest of vitamin premix, trace element premix and other auxiliary materials; wherein the traditional Chinese medicine component is composed of radix astragali, radix ophiopogonis, fructus schisandrae, radix salviae miltiorrhizae and poria cocos.The composition can significantly reduce the myocardial thickness and myocardial injury markers of subclinical cats, has good palatability, and is suitable for long-term feeding of HCM high-risk cats and subclinical cats.

Description

Technical Field

[0001] This invention relates to the field of pet food technology, specifically to a composition for preventing feline hypertrophic cardiomyopathy and its preparation method. Background Technology

[0002] Hypertrophic cardiomyopathy (HCM) is the most common heart disease in cats, affecting approximately 15% of all felines, and its prevalence increases with age. Characterized by concentric hypertrophy of the left ventricle, it can lead to heart failure, arterial thromboembolism, and even sudden death. Currently, there are no proven standard drug therapies that can alter the long-term prognosis of cats with preclinical HCM.

[0003] In recent years, the role of nutritional management in the prevention and control of hepatocellular carcinoma (HCM) has received increasing attention. Studies have shown that cats with HCM exhibit specific metabolic abnormalities, including insulin / insulin-like growth factor-1 (IGF-1) axis dysregulation, systemic inflammation, and elevated oxidative stress levels. High levels of IGF-1 can stimulate cardiomyocyte protein synthesis and promote ventricular hypertrophy. Therefore, regulating these metabolic pathways through nutritional intervention may be a novel strategy for preventing or delaying the progression of HCM. However, no relevant research has been published to date. Summary of the Invention

[0004] The purpose of this invention is to provide a composition for preventing hypertrophic cardiomyopathy in cats. This composition is palatable and effectively reduces HCM risk factors through the dual effects of nutritional regulation and traditional Chinese medicine conditioning.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: a composition for preventing feline hypertrophic cardiomyopathy, comprising, by weight percentage: 30%-50% animal protein source, 10%-20% animal fat source, 3%-8% dietary fiber, 5%-15% starch, 1%-5% functional nutrient components, 1%-3% traditional Chinese medicine components, with the remainder being vitamin premix and trace element premix, and other excipients; wherein the traditional Chinese medicine components are composed of Astragalus membranaceus, Ophiopogon japonicus, Schisandra chinensis, Salvia miltiorrhiza, and Poria cocos.

[0006] This invention achieves a holistic intervention on the multidimensional pathogenesis of feline hypertrophic cardiomyopathy by systematically integrating a nutritional framework with specific Chinese herbal medicine components.

[0007] At the nutritional level, this invention adjusts the proportions of animal protein, animal fat, dietary fiber, and starch, particularly strictly limiting starch to 5%-15%. This reduces postprandial blood glucose fluctuations and inhibits excessive activation of the insulin / IGF-1 axis, thereby reducing IGF-1 stimulation of cardiomyocyte protein synthesis and mitigating the pathological drivers of myocardial hypertrophy at its source. Simultaneously, the high proportion of animal protein ensures the basic nutritional needs of cats as obligate carnivores, preventing energy deficiency due to sugar restriction. The addition of dietary fiber further stabilizes the blood glucose curve by regulating gut microbiota and delaying gastric emptying, creating a metabolic synergy with the low-starch strategy.

[0008] At the functional nutrition level, the introduction of functional nutritional components provides precise intervention targeting the inflammatory and oxidative stress pathological characteristics of HCM. Among them, fish oil rich in EPA and DHA competitively inhibits the arachidonic acid metabolic pathway, reducing the production of pro-inflammatory prostaglandins and leukotrienes, and alleviating chronic inflammatory infiltration of myocardial tissue; taurine, as an essential amino acid for cats, maintains calcium homeostasis and osmotic pressure balance in myocardial cells, preventing myocardial dilation caused by taurine deficiency; L-carnitine promotes the entry of long-chain fatty acids into mitochondria for β-oxidation, improves myocardial energy metabolism efficiency, and alleviates the myocardial energy crisis commonly seen in cats with HCM.

[0009] At the level of traditional Chinese medicine intervention, the combination of Astragalus membranaceus, Ophiopogon japonicus, Schisandra chinensis, Salvia miltiorrhiza, and Poria cocos is not simply an addition of Chinese herbs, but a targeted formula based on the TCM concept of Qi deficiency and blood stasis. Astragalus membranaceus tonifies Qi and strengthens the exterior, directly counteracting the Qi deficiency pathogenesis of HCM by enhancing myocardial contractility and improving energy metabolism; Ophiopogon japonicus nourishes Yin and generates fluids, improving energy metabolism under myocardial ischemia and hypoxia, and regulating immune function; Schisandra chinensis benefits Qi and generates fluids, calms the mind and soothes the nerves, and its strong antioxidant activity can eliminate excess free radicals generated during the pathological process of HCM; Salvia miltiorrhiza invigorates blood and removes blood stasis, alleviating the blood stasis state of myocardial tissue by improving microcirculation and inhibiting platelet aggregation; Poria cocos strengthens the spleen and calms the mind, promotes diuresis and eliminates dampness, and provides early prevention of water and sodium retention that may occur in the later stages of HCM.

[0010] The "other additives" mentioned here refer to the common additives added to cat food, such as emulsifiers, binders, calcium sources, etc.

[0011] Preferably, the traditional Chinese medicine components, by weight, are as follows: Astragalus membranaceus 3-5 parts, Ophiopogon japonicus 2-4 parts, Schisandra chinensis 1-3 parts, Salvia miltiorrhiza 2-4 parts, and Poria cocos 2-3 parts.

[0012] Astragalus is the principal herb, used in the largest quantity (3-5 parts), focusing on tonifying qi and strengthening the exterior, supporting the body's resistance and consolidating its foundation. Its positive inotropic effect and ability to improve energy metabolism set the tone for the entire formula. Ophiopogon japonicus is the assistant herb (2-4 parts), nourishing yin and generating fluids. Together with Astragalus, it tonifies both qi and yin, targeting the pathological evolution of qi and yin deficiency in the course of HCM. Schisandra chinensis is the adjuvant herb (1-3 parts), used in a relatively light quantity. Its astringent properties calm the mind, nourish qi and generate fluids, and exert a strong antioxidant effect. Salvia miltiorrhiza is the assistant herb (2-4 parts), promoting blood circulation and removing blood stasis. Together with the qi-tonifying effect of Astragalus, it forms a synergistic effect of "where qi flows, blood flows," directly targeting the blood stasis pathogenesis of myocardial hypertrophy. Poria cocos is the adjuvant herb (2-3 parts), strengthening the spleen, calming the mind, and promoting diuresis and dampness elimination. It not only assists Astragalus in strengthening the spleen and nourishing qi, but also prevents Ophiopogon japonicus from being too cloying and hindering the spleen. At the same time, it provides early intervention for the potential risk of heart failure.

[0013] This formula has been optimized to avoid stagnation caused by simply replenishing qi, or qi deficiency caused by simply promoting blood circulation, achieving a balanced formula that replenishes without causing stagnation and promotes blood circulation without causing harm. Clinical observations show that within this formula range, the improvement in interventricular septal thickness and left ventricular posterior wall thickness in cats with HCM is most significant, and no adverse reactions such as gastrointestinal discomfort caused by the properties of traditional Chinese medicine have been observed.

[0014] Preferably, the functional nutritional components include at least fish oil, taurine, and L-carnitine; the fish oil is rich in eicosapentaenoic acid and docosahexaenoic acid, and the amount of fish oil added accounts for 0.5%-2% of the total mass of the composition. When it is less than 0.5%, the anti-inflammatory effect is not significant; when it is more than 2%, palatability may decrease due to fat oxidation, and excessive polyunsaturated fatty acids may increase the risk of oxidative stress.

[0015] Preferably, the amount of taurine added is not less than 0.2% of the total mass of the composition, on a dry matter basis. The taurine addition of not less than 0.2% is based on the absolute requirement of taurine for cats as obligate carnivores. Taurine participates in calcium ion regulation, osmotic pressure maintenance, and antioxidant defense in cardiomyocytes; deficiency can lead to dilated cardiomyopathy. In HCM prevention, adequate taurine helps maintain the electrophysiological stability and contractile function of cardiomyocytes.

[0016] Preferably, the amount of L-carnitine added is 0.05%-0.2% of the total mass of the composition. Hypertrophic cardiomyopathy in cats with hemorrhage (HCM) leads to relative ischemia and hypoxia, resulting in decreased efficiency of fatty acid oxidation for energy. The introduction of L-carnitine addresses the energy metabolism disorder in HCM. As an essential carrier for fatty acids to enter mitochondria, L-carnitine promotes β-oxidation of long-chain fatty acids, increases ATP production efficiency, and alleviates the myocardial energy crisis.

[0017] Preferably, the animal protein source includes at least one of chicken meal and fish meal; the animal fat source includes chicken fat; the dietary fiber includes pea fiber; and the starch includes tapioca starch. Chicken meal and fish meal are commonly used high-quality protein sources in pet food, with balanced amino acid composition, high bioavailability, and good palatability. Chicken fat is rich in monounsaturated fatty acids, has good stability and flavor, and is a preferred fat source for cats. Pea fiber, as a soluble dietary fiber, has good water-holding and gelling properties, which can slow gastric emptying, stabilize the blood sugar curve, and synergize with a low-starch strategy. Compared with grain starches (such as corn and wheat), tapioca starch has a lower glycemic index and better gluten-free properties, making it suitable for cats sensitive to grains. Its gelatinization properties are also suitable for extrusion processing. This combination of ingredients ensures nutritional efficacy while considering the feasibility of industrial production and the palatability of the product.

[0018] Preferably, the dosage form of the composition is puffed pellets, baked grains, or wet canned grains.

[0019] The present invention also provides a method for preparing the composition, characterized by comprising the following steps: (1) Raw material pretreatment: After cleaning and drying the Chinese herbal components, they are pulverized and sieved to obtain Chinese herbal micro powder for later use; (2) Mixing: Mix the animal protein source, starch, and dietary fiber dry ingredients evenly; mix the animal fat source and some fish oil and other liquid ingredients; (3) Grinding and homogenization: The mixed dry raw materials are subjected to ultra-fine grinding; (4) Ingredients: Place the wet raw materials and dry raw materials in a mixer, and add the Chinese herbal powder, taurine, L-carnitine, vitamins and trace elements at the same time, and mix thoroughly; (5) Extrusion and drying: The mixture is fed into an extruder for extrusion and cooking, and then dried and cooled; (6) Spraying: Spray the remaining fish oil components onto the surface of the cooled granules to obtain the finished product.

[0020] Preferably, in step (1), the pulverization is performed by passing through an 80-100 mesh sieve; in step (3), the fineness of the ultrafine pulverization reaches 60-80 mesh.

[0021] The present invention also provides the use of the composition in the preparation of pet food for the prevention of feline hypertrophic cardiomyopathy. Detailed Implementation

[0022] The technical solutions of the present invention will be clearly and completely described below with reference to specific embodiments.

[0023] Example 1 The formula for preparing 100 kg of extruded pellet food for preventing hepatic muscular dystrophy in cats is as follows: Chicken meal 35 kg, fish meal 10 kg, tapioca starch 12 kg, chicken oil 8 kg, fish oil 1.5 kg, pea fiber 5 kg, Chinese herbal powder 2 kg (including Astragalus membranaceus 0.8 kg, Ophiopogon japonicus 0.5 kg, Schisandra chinensis 0.2 kg, Salvia miltiorrhiza 0.3 kg, Poria cocos 0.2 kg), taurine 0.3 kg, L-carnitine 0.1 kg, vitamin premix 0.5 kg, trace element premix 0.5 kg, and other excipients to make up to 100 kg.

[0024] Example 2 The formula for preparing 100 kg of extruded pellet food for preventing hepatic muscular dystrophy in cats is as follows: Chicken meal 35 kg, fish meal 10 kg, tapioca starch 10 kg, chicken oil 10 kg, fish oil 1.5 kg, pea fiber 5 kg, Chinese herbal powder 2 kg (including Astragalus membranaceus 0.6 kg, Ophiopogon japonicus 0.4 kg, Schisandra chinensis 0.2 kg, Salvia miltiorrhiza 0.4 kg, Poria cocos 0.4 kg), taurine 0.3 kg, L-carnitine 0.1 kg, vitamin premix 0.5 kg, trace element premix 0.5 kg, and other excipients to make up to 100 kg.

[0025] Example 3 The formula for preparing 100 kg of extruded pellet food for preventing hepatic muscular dystrophy in cats is as follows: Chicken meal 44 kg, tapioca starch 5 kg, chicken oil 10 kg, fish oil 2 kg, pea fiber 3 kg, Chinese herbal powder 3 kg (including Astragalus membranaceus 1.25 kg, Ophiopogon japonicus 0.5 kg, Schisandra chinensis 0.25 kg, Salvia miltiorrhiza 0.5 kg, Poria cocos 0.5 kg), taurine 0.3 kg, L-carnitine 0.2 kg, vitamin premix 0.5 kg, trace element premix 0.5 kg, and other excipients to make up to 100 kg.

[0026] Example 4 The formula for preparing 100 kg of extruded pellet food for preventing hepatic muscular dystrophy in cats is as follows: Chicken meal 20kg, fish meal 15kg, tapioca starch 12kg, chicken oil 16kg, fish oil 1.5kg, pea fiber 6kg, Chinese herbal powder 2.5kg (including Astragalus membranaceus 0.7kg, Ophiopogon japonicus 0.5kg, Schisandra chinensis 0.3kg, Salvia miltiorrhiza 0.6kg, Poria cocos 0.4kg), taurine 0.22kg, L-carnitine 0.08kg, vitamin premix 0.5kg, trace element premix 0.5kg, and other excipients to make up to 100kg.

[0027] Example 5 The formula for preparing 100 kg of extruded pellet food for preventing hepatic muscular dystrophy in cats is as follows: Chicken meal 45kg, tapioca starch 8kg, chicken oil 12kg, fish oil 1.8kg, pea fiber 4kg, Chinese herbal powder 1.5kg (including Astragalus membranaceus 0.7kg, Ophiopogon japonicus 0.5kg, Schisandra chinensis 0.3kg, Salvia miltiorrhiza 0.6kg, Poria cocos 0.4kg), taurine 0.28kg, L-carnitine 0.15kg, vitamin premix 0.5kg, trace element premix 0.5kg, and other excipients to make up to 100kg.

[0028] Comparative Example 1 The difference from Example 1 is that no traditional Chinese medicine components are added. The formula for preparing 100 kg of puffed pellets is as follows: Chicken meal 37kg, fish meal 10kg, tapioca starch 12kg, chicken fat 8kg, fish oil (EPA+DHA≥30%) 1.5kg, pea fiber 5kg, taurine 0.3kg, L-carnitine 0.1kg, vitamin premix 0.5kg, trace element premix 0.5kg, and other auxiliary ingredients to make up to 100kg.

[0029] Comparative Example 2 The formula for preparing 100 kg of extruded pelleted grain is as follows: Chicken meal 30kg, fish meal 10kg, tapioca starch 25kg (consistent cat food starch content), chicken oil 8kg, fish oil (EPA+DHA≥30%) 1.5kg, pea fiber 3kg, Chinese herbal powder 2kg (composition same as in Example 1: Astragalus 0.8kg, Ophiopogon japonicus 0.5kg, Schisandra chinensis 0.2kg, Salvia miltiorrhiza 0.3kg, Poria cocos 0.2kg), taurine 0.3kg, L-carnitine 0.1kg, vitamin premix 0.5kg, trace element premix 0.5kg, and other excipient mixtures to make up to 100kg.

[0030] Comparative Example 3 The formula for preparing 100 kg of extruded pelleted grain is as follows: Chicken meal 35kg, fish meal 10kg, tapioca starch 12kg, chicken oil 8kg, fish oil 1.5kg, pea fiber 5kg, substitute Chinese herbal powder 2kg (including ginseng 0.5kg, angelica 0.5kg, chuanxiong 0.4kg, safflower 0.3kg, licorice 0.3kg), taurine 0.3kg, L-carnitine 0.1kg, vitamin premix 0.5kg, trace element premix 0.5kg, and other excipient mixtures to make up to 100kg.

[0031] In Examples 1-5 and Comparative Examples 1-4, the vitamin premix composition was as follows: each 0.5 kg of vitamin premix contained 800 mg of vitamin A acetate, 100 mg of vitamin D3, 5 g of DL-α-tocopherol acetate, 100 mg of sodium menadione bisulfite (vitamin K3), 500 mg of thiamine hydrochloride (vitamin B1), 500 mg of riboflavin (vitamin B2), 400 mg of pyridoxine hydrochloride (vitamin B6), 5 mg of cyanocobalamin (vitamin B12), 3 g of niacin, 1.5 g of D-calcium pantothenate, 50 mg of folic acid, 20 mg of biotin, 50 g of choline chloride, and 500 mg of antioxidant BHT, with the remainder being corn starch carrier, totaling 0.5 kg.

[0032] The composition of the trace element premix is ​​as follows: each 0.5kg of trace element premix contains 25g of ferrous sulfate, 3g of copper sulfate, 12g of manganese sulfate, 30g of zinc sulfate, 400mg of calcium iodate, 100mg of sodium selenite, and 50mg of cobalt chloride, with the remainder being zeolite powder carrier, totaling 0.5kg.

[0033] Other excipient mixture: Prepared by uniformly mixing lecithin, dicalcium phosphate and guar gum in a mass ratio of 1:3:1.

[0034] The extruded pellets of Examples 1-5 and Comparative Examples 1-4 were prepared according to the following method (adjustments were made to the components and steps in different examples and comparative examples): (1) Raw material pretreatment: After washing and drying Astragalus membranaceus, Ophiopogon japonicus, Schisandra chinensis, Salvia miltiorrhiza and Poria cocos, they are pulverized and passed through an 80-mesh sieve to obtain Chinese medicine powder for later use; (2) Mixing: Mix the dry ingredients such as chicken powder, fish powder (if any), tapioca starch, and pea fiber evenly; mix the liquid ingredients such as chicken oil and some fish oil; (3) Crushing and homogenization: The mixed dry raw materials are ultra-finely crushed to a fineness of 60 mesh; (4) Ingredients: Place the wet raw materials and dry raw materials in a mixer, and add the Chinese herbal powder, taurine, L-carnitine, other excipient mixtures, vitamin premix and trace element premix, and mix thoroughly; (5) Extrusion and drying: The mixture is fed into a twin-screw extruder for extrusion and maturation treatment (temperature 120-140℃, pressure 3-5MPa), and then dried and cooled; (6) Spraying: Spray the remaining fish oil (already included in the total amount of the formula) onto the surface of the cooled granules. (7) Packaging: Measuring and packaging to obtain the finished puffed grain product.

[0035] Effect verification 1. Laboratory animals Ninety adult domestic cats (aged 2-8 years, weighing 3.5-6.0 kg, half male and half female) diagnosed with subclinical hypertrophic cardiomyopathy (sHCM) by echocardiography were selected and randomly divided into 9 groups according to weight, age and disease severity, corresponding to each example and comparative example, with 10 cats in each group.

[0036] 2. Feeding and Management All experimental cats were housed individually in controlled environments (temperature 22±2℃, relative humidity 55±10%), with free access to water daily and a fixed amount of food (4% of body weight dry matter basal). Feeding was observed and recorded daily. The experimental period was 6 months.

[0037] 3. Detection Indicators and Methods 3.1 Cardiac Indicators The detection indicators are: interventricular septal thickness during diastole (IVSd), left ventricular posterior wall thickness during diastole (LVWd), left ventricular end-diastolic diameter (LVIDd), left ventricular end-systolic diameter (LVIDs), left ventricular ejection fraction (EF%), and left ventricular shortening fraction (FS%).

[0038] Testing Methods: Transthoracic echocardiography (TCO) was performed by the same experienced veterinarian at months 0, 3, and 6 of the trial, using a high-frequency color Doppler ultrasound diagnostic instrument (probe frequency 7.5-12MHz). The cats were positioned on their left lateral decubitus position, and measurements were taken in the long and short axis sections parasternal to the right side. Each measurement was taken over three cardiac cycles, and the average value was calculated. Diagnostic criteria were based on the American College of Veterinary Medicine (ACVIM) guidelines for feline cardiomyopathy: IVSd or LVWd > 5.5 mm suggests myocardial hypertrophy.

[0039] 3.2 Serum biochemical indicators Detection indicators: serum insulin-like growth factor-1 (IGF-1), cardiac troponin I (cTnI), high-sensitivity C-reactive protein (hs-CRP), malondialdehyde (MDA), superoxide dismutase (SOD), and total antioxidant capacity (T-AOC).

[0040] Detection method: IGF-1: Enzyme-linked immunosorbent assay (ELISA) was used with a cat-specific IGF-1 detection kit. The procedure was followed according to the instructions. The absorbance value was read at a wavelength of 450 nm using an ELISA reader, and quantification was performed using a standard curve.

[0041] cTnI: Detected using a chemiluminescent immunoassay with a high-sensitivity cardiac troponin I detection kit and automated by a chemiluminescent immunoassay analyzer. The detection limit is 0.01 ng / mL.

[0042] hs-CRP: Detected using immunoturbidimetric assay and a fully automated biochemical analyzer.

[0043] MDA: The absorbance of serum reacted with TBA was measured at 532 nm using the thiobarbituric acid (TBA) colorimetric method, and the MDA content was calculated according to the standard curve.

[0044] SOD: The absorbance was measured at 550 nm using the xanthine oxidase method (hydroxylamine oxidation method) according to the kit instructions.

[0045] T-AOC: The absorbance was measured at 593 nm using the ferric reduction method (FRAP method) and calculated according to the standard curve.

[0046] 3.3 Evaluation of palatability and coat luster The detection indicators are: active feeding rate, average daily feed intake, weight change, coat gloss score, and mental state score.

[0047] Detection method: Active feeding rate: Record the number of cats that actively feed each day / the total number of cats × 100%, and take the average value after 30 consecutive days of observation.

[0048] Average daily feed intake: A fixed amount of feed was given each day, and the remaining amount was weighed the next day to calculate the actual feed intake. The average value during the trial period was taken.

[0049] 4. Test Results Table 1 Results of cardiac marker tests Note: This indicates a statistically significant difference compared to month 0 (P < 0.05).

[0050] Table 2. Changes in serum parameters of cats in each group of experiments. Note: This indicates a statistically significant difference compared to month 0 (P < 0.05).

[0051] Table 3 Palatability evaluation of each group of experimental cats

Claims

1. A composition for preventing feline hypertrophic cardiomyopathy, characterized in that, By weight percentage, it includes: 30%-50% animal protein source, 10%-20% animal fat source, 3%-8% dietary fiber, 5%-15% starch, 1%-5% functional nutrient components, 1%-3% traditional Chinese medicine components, and the remainder being vitamin premix, trace element premix and other excipients; wherein, the traditional Chinese medicine components are composed of Astragalus membranaceus, Ophiopogon japonicus, Schisandra chinensis, Salvia miltiorrhiza and Poria cocos.

2. The composition according to claim 1, characterized in that, The herbal ingredients, by weight, are: Astragalus membranaceus 3-5 parts, Ophiopogon japonicus 2-4 parts, Schisandra chinensis 1-3 parts, Salvia miltiorrhiza 2-4 parts, and Poria cocos 2-3 parts.

3. The composition according to claim 1, characterized in that, The functional nutritional components include at least fish oil, taurine, and L-carnitine; the fish oil is rich in eicosapentaenoic acid and docosahexaenoic acid, and the amount of fish oil added accounts for 0.5%-2% of the total mass of the composition.

4. The composition according to claim 3, characterized in that, The amount of taurine added shall not be less than 0.2% of the total mass of the composition, on a dry matter basis.

5. The composition according to claim 3, characterized in that, The amount of L-carnitine added is 0.05%-0.2% of the total mass of the composition.

6. The composition according to claim 1, characterized in that, The animal protein source includes at least one of chicken meal and fish meal; the animal fat source includes chicken fat; the dietary fiber includes pea fiber; and the starch includes tapioca starch.

7. The composition according to any one of claims 1-6, characterized in that, The composition is available in the form of puffed pellets, baked grains, or wet canned grains.

8. A method for preparing the composition according to any one of claims 1-7, characterized in that, Includes the following steps: (1) Raw material pretreatment: After cleaning and drying the Chinese herbal components, they are pulverized and sieved to obtain Chinese herbal micro powder for later use; (2) Mixing: Mix the animal protein source, starch, and dietary fiber dry ingredients evenly; mix the animal fat source and some fish oil and other liquid ingredients; (3) Grinding and homogenization: The mixed dry raw materials are subjected to ultra-fine grinding; (4) Ingredients: Place the wet raw materials and dry raw materials in a mixer, and add the Chinese herbal powder, taurine, L-carnitine, vitamins and trace elements at the same time, and mix thoroughly; (5) Extrusion and drying: The mixture is fed into an extruder for extrusion and cooking, and then dried and cooled; (6) Spraying: Spray the remaining heat-sensitive fish oil components onto the cooled granules to obtain the finished product.

9. The method according to claim 8, characterized in that, In step (1), the pulverization is performed by passing through an 80-100 mesh sieve; in step (3), the fineness of the ultrafine pulverization reaches 60-80 mesh.

10. Use of the composition of any one of claims 1-6 in the preparation of pet food for the prevention of feline hypertrophic cardiomyopathy.

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