Feeding of laying hens with ornithine-aspartate dipeptide and application

Abstract

The application belongs to the technical field of livestock and poultry feed and provides a laying hen feed containing ornithine-aspartic acid dipeptide and application, which comprises corn-soybean meal type basic daily ration and ornithine-aspartic acid active additive added to the corn-soybean meal type basic daily ration, wherein the ornithine-aspartic acid active additive is an additive containing ornithine and aspartic acid structural units prepared in advance; the ornithine-aspartic acid active additive is added to the corn-soybean meal type basic daily ration, and the addition amount is controlled to be 450-550 mg / kg, preferably 500 mg / kg. The scheme is different from the way of adding ornithine and aspartic acid separately, and can make the two structural units enter the feed system in the form of the active additive prepared in advance, so as to improve the stability of nutrition regulation of laying hens in the middle and late stages of egg production.

Description

Technical Field

[0001] This invention belongs to the field of livestock and poultry feed technology, and particularly relates to a laying hen feed containing ornithine-aspartic acid dipeptide and its application. Background Technology

[0002] As laying hens enter the mid-to-late stages of egg production, their metabolic capacity gradually declines with increasing age, while the burden on liver lipid and nitrogen metabolism increases. This can easily lead to problems such as decreased egg production rate, lower average egg weight, higher feed conversion ratio, and poorer egg quality. Especially during the continuous egg production of high-producing hens, yolk formation requires a large amount of lipids and nutrients. Insufficient nutritional regulation can easily affect quality indicators such as albumen height, Haugh unit, eggshell thickness, and yolk index, thereby reducing the quality of marketable eggs and the economic benefits of egg farming.

[0003] Current laying hen feeds are mostly based on corn-soybean meal basal diets, and maintain egg production needs by supplementing amino acids, vitamins and trace elements. Ornithine and aspartic acid are related to the regulation of nitrogen metabolism, amino acid metabolism and liver metabolism in animals. Although adding ornithine and aspartic acid alone can improve nutritional metabolic status to a certain extent, there are still shortcomings in terms of mixing uniformity, intake synchronization and metabolic utilization stability when adding them separately, making it difficult to give full play to their synergistic improvement effect. Summary of the Invention

[0004] This invention provides a laying hen feed containing ornithine-aspartic acid active additives and its application, aiming to solve the problems of decreased egg production rate, reduced average egg weight, increased feed conversion ratio, and decreased egg quality in laying hens during the mid-to-late stages of egg production as they age. At the same time, it solves the problem that when ornithine and aspartic acid are added separately in the prior art, the synchronization between the two during feed mixing, feed intake, and body metabolism is insufficient, the improvement effect is not stable, and it is difficult to simultaneously improve egg production performance and egg quality.

[0005] This invention is achieved as follows: a layer hen feed containing ornithine-aspartic acid dipeptide and its application, comprising a corn-soybean meal basal diet and an ornithine-aspartic acid active additive added to the corn-soybean meal basal diet. The ornithine-aspartic acid active additive is a pre-prepared additive containing ornithine and aspartic acid structural units. The amount of the ornithine-aspartic acid active additive added to the layer hen feed is 450-550 mg / kg. The layer hen feed is used for continuous feeding of layer hens after 37 weeks of age.

[0006] Preferably, the amount of the ornithine-aspartic acid active additive added to the laying hen feed is 500 mg / kg.

[0007] Preferably, the corn-soybean meal basal diet includes energy feed, protein feed, calcium and phosphorus supplement feed, amino acid supplement feed, vitamin premix and trace element premix.

[0008] Preferably, the energy source includes corn, soybean oil, and wheat bran; the protein source includes soybean meal and corn gluten meal; the calcium and phosphorus supplement includes dicalcium phosphate, limestone powder, and limestone granules; and the amino acid supplement includes L-lysine hydrochloride, DL-methionine, and alanine.

[0009] Preferably, based on 100 parts by weight of the corn-soybean meal basal diet, the corn-soybean meal basal diet includes 60.98 parts corn, 22.89 parts soybean meal, 2.78 parts corn gluten meal, 1.02 parts soybean oil, 0.98 parts wheat bran, 0.12 parts L-lysine hydrochloride, 1.42 parts dicalcium phosphate, 4.42 parts limestone powder, 4.42 parts limestone granules, 0.14 parts choline chloride, 0.13 parts DL-methionine, 0.03 parts antioxidant, 0.35 parts salt, 0.03 parts vitamin premix, 0.20 parts trace element premix, and 0.10 parts alanine.

[0010] Preferably, the calculated nutrient levels of the corn-soybean meal basal diet include metabolizable energy 2.70-2.76 Mcal / kg, crude protein 16.50%-17.00%, calcium 3.70%-4.00%, total phosphorus 0.60%-0.70%, available phosphorus 0.35%-0.45%, lysine 0.70%-0.80%, and methionine 0.34%-0.42%.

[0011] Preferably, the calculated nutrient levels of the corn-soybean meal basal diet include metabolizable energy 2.73 Mcal / kg, crude protein 16.76%, calcium 3.87%, total phosphorus 0.65%, available phosphorus 0.39%, lysine 0.75%, and methionine 0.38%.

[0012] Preferably, the ornithine-aspartic acid active additive is added to the corn-soybean meal basal diet by a graded premixing method. The graded premixing method includes first mixing the ornithine-aspartic acid active additive with a portion of the corn-soybean meal basal diet to form a premix, and then mixing the premix with the remaining corn-soybean meal basal diet.

[0013] Preferably, the laying hens are Lohmann pink laying hens aged 37 weeks or older, and the laying hen feed is continuously fed during the 39-51 week age period. The improvement of laying hen egg production performance includes increasing the laying rate, increasing the average egg weight, and reducing the feed conversion ratio.

[0014] Preferably, the laying hens are Lohmann Pink laying hens aged 37 weeks or older, and the improvement of egg quality includes increasing at least one of the following: yolk index, albumen height, Haugh unit, egg shape index, and eggshell thickness.

[0015] Compared with related technologies, the laying hen feed containing ornithine-aspartic acid dipeptide provided by the present invention and its application have the following beneficial effects:

[0016] 1. This invention adds ornithine-aspartic acid active additive to corn-soybean meal basal diet and controls the addition amount to 450-550 mg / kg, preferably 500 mg / kg. This method is different from adding ornithine and aspartic acid separately. It allows the two structural units to enter the feed system in the form of pre-prepared active additives, thereby improving the stability of nutritional regulation of laying hens in the middle and late stages of egg production.

[0017] 2. This invention uses a conventional corn-soybean meal basal diet as a carrier. The basal diet includes energy raw materials, protein raw materials, calcium and phosphorus supplementary raw materials, amino acid supplementary raw materials, vitamin premix and trace element premix. It does not require significant adjustment to the original diet structure of the farm. By adding ornithine-aspartic acid active additives, it can be used for continuous feeding of laying hens after 37 weeks of age, and has good production adaptability.

[0018] 3. This invention introduces ornithine-aspartic acid active additives through a graded premixing method. First, it is mixed with a portion of the corn-soybean meal basal diet to form a premix, and then mixed with the remaining basal diet. This helps to improve the uniformity of the low-added active ingredients in the complete feed, reduce the problem of excessively high or low local concentrations, and enable laying hens to obtain a more stable effective intake during continuous feeding.

[0019] 4. This invention is applicable to Lohmann Pink laying hens aged 39-51 weeks. It can be used to improve egg production rate, increase average egg weight and reduce feed conversion ratio. At the same time, it can also improve egg quality indicators such as yolk index, albumen height, Haugh unit, egg shape index and eggshell thickness. Compared with conventional programs that simply emphasize amino acid supplementation, this invention takes into account both egg production performance and egg quality improvement, and has clear application targets and feeding stages. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the preparation and application process of the laying hen feed containing ornithine-aspartic acid active additive of the present invention.

[0021] Figure 2 This is a schematic diagram of the composition of the laying hen feed containing ornithine-aspartic acid active additive of the present invention. Detailed Implementation

[0022] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs; the terminology used herein in the specification of the application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application; the terms "comprising" and "having," and any variations thereof, in the specification, claims, and foregoing drawings of this application are intended to cover non-exclusive inclusion. The terms "first," "second," etc., in the specification, claims, or foregoing drawings of this application are used to distinguish different objects, not to describe a particular order.

[0023] In this document, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0024] Example 1

[0025] The present invention provides a preferred embodiment of an ornithine-aspartic acid active additive for laying hens and its application, for example... Figures 1 to 2 As shown: A layer hen feed containing ornithine-aspartic acid active additive, comprising a corn-soybean meal basal diet and an ornithine-aspartic acid active additive added to the corn-soybean meal basal diet, wherein the ornithine-aspartic acid active additive is a pre-prepared additive containing ornithine and aspartic acid structural units, and the amount of the ornithine-aspartic acid active additive added to the layer hen feed is 450-550 mg / kg.

[0026] In this embodiment, the ornithine-aspartic acid active additive is preferably ornithine-aspartic acid OA, and the preferred addition amount is 500 mg / kg. By adding ornithine and aspartic acid to the basal diet in the form of pre-prepared active additives, it is possible to differentiate them from adding ornithine and aspartic acid separately, so that the two structural units have better synchronicity and stability in feed mixing, intake and nutrient metabolism, thus making it suitable for continuous feeding of laying hens in the mid-to-late stages of egg production.

[0027] In a further preferred embodiment of the present invention, the corn-soybean meal basal diet includes energy feed, protein feed, calcium and phosphorus supplement feed, amino acid supplement feed, vitamin premix and trace element premix.

[0028] In this embodiment, the energy feedstock includes corn, soybean oil, and wheat bran; the protein feedstock includes soybean meal and corn gluten meal; the calcium and phosphorus supplements include dicalcium phosphate, limestone powder, and limestone granules; and the amino acid supplements include L-lysine hydrochloride, DL-methionine, and alanine. By combining these feedstocks, basic energy, protein, calcium and phosphorus minerals, essential amino acids, vitamins, and trace elements can be provided to laying hens in the mid-to-late stages of egg production. This allows the ornithine-aspartic acid active additive to function effectively in a conventional laying hen diet system without significantly altering the existing diet structure of the farm.

[0029] In a further preferred embodiment of the present invention, taking 100 parts by weight of a corn-soybean meal basal diet, the corn-soybean meal basal diet includes 60.98 parts corn, 22.89 parts soybean meal, 2.78 parts corn gluten meal, 1.02 parts soybean oil, 0.98 parts wheat bran, 0.12 parts L-lysine hydrochloride, 1.42 parts dicalcium phosphate, 4.42 parts limestone powder, 4.42 parts limestone granules, 0.14 parts choline chloride, 0.13 parts DL-methionine, 0.03 parts antioxidant, 0.35 parts salt, 0.03 parts vitamin premix, 0.20 parts trace element premix, and 0.10 parts alanine.

[0030] In this embodiment, the ornithine-aspartic acid active additive is an external component, and its addition amount is calculated per kilogram of complete laying hen feed. By using a basal diet of 100 parts and treating the ornithine-aspartic acid active additive as an external component, the active additive can be avoided from being mistakenly considered as part of the percentage composition of the basal diet, making the feed composition relationship clearer.

[0031] In a further preferred embodiment of the present invention, the calculated nutrient levels of the corn-soybean meal basal diet include metabolizable energy 2.70-2.76 Mcal / kg, crude protein 16.50%-17.00%, calcium 3.70%-4.00%, total phosphorus 0.60%-0.70%, available phosphorus 0.35%-0.45%, lysine 0.70%-0.80%, and methionine 0.34%-0.42%.

[0032] In this embodiment, the preferred calculated nutrient levels of the corn-soybean meal basal diet are: metabolizable energy 2.73 Mcal / kg, crude protein 16.76%, calcium 3.87%, total phosphorus 0.65%, available phosphorus 0.39%, lysine 0.75%, and methionine 0.38%. By limiting the nutrient levels of the basal diet, it can be demonstrated that the present invention does not simply involve arbitrarily adding nutrients, but rather further improves egg production performance and egg quality by adding ornithine-aspartic acid active additives to a diet system that meets the basic nutritional needs of laying hens in the mid-to-late stages of egg production.

[0033] In a further preferred embodiment of the present invention, the ornithine-aspartic acid active additive is added to the corn-soybean meal basal diet by a graded premixing method. The graded premixing method includes first mixing the ornithine-aspartic acid active additive with a portion of the corn-soybean meal basal diet to form a premix, and then mixing the premix with the remaining corn-soybean meal basal diet.

[0034] In this embodiment, since the amount of ornithine-aspartic acid active additive added is 450-550 mg / kg, which is a low-dose active additive, if it is directly added to the entire basal diet, uneven local dispersion may easily occur. By using graded premixing, the initial dispersion uniformity of the active additive in a small amount of basal diet can be improved first, and then it can be diffused into the entire diet through secondary mixing, thereby reducing the problem of excessively high or low local concentrations and ensuring that laying hens obtain a relatively stable effective intake during continuous feeding.

[0035] Example 2

[0036] Based on Example 1, the preferred embodiment of the laying hen feed containing ornithine-aspartic acid active additive provided by the present invention and its application are as follows: Figures 1 to 2 As shown: The laying hen feed prepared in Example 1 was used for continuous feeding of laying hens after 37 weeks of age, preferably for Lohmann pink laying hens aged 39-51 weeks, to improve the laying performance of the hens and improve the quality of the eggs.

[0037] In this embodiment, the laying hen feed is applied to laying hens in the mid-to-late laying stage, preferably Lohmann Powder laying hens older than 37 weeks. Due to increased age, continued egg production, and increased liver metabolic burden, laying hens in the mid-to-late laying stage are prone to problems such as decreased egg production rate, decreased average egg weight, increased feed conversion ratio, and reduced egg quality. This invention addresses these production problems by adding 450-550 mg / kg ornithine-aspartic acid active additive to a conventional corn-soybean meal basal diet.

[0038] In a further preferred embodiment of the present invention, the laying hens are fed continuously from 39 to 51 weeks of age, with free access to feed and water, and are raised in a tiered cage system. The feeding and management are carried out in accordance with the conventional feeding and management requirements for laying hens.

[0039] In this embodiment, the continuous feeding process includes a pre-feeding stage and a formal feeding stage. During the pre-feeding stage, a basal diet is fed to allow the laying hens to adapt to the rearing environment and basal diet conditions. During the formal feeding stage, the laying hens are fed laying hen feed containing ornithine-aspartic acid active additives, and the egg production, egg weight, number of deformed eggs, and feed intake are recorded in repeated units to calculate the laying rate, average egg weight, average daily feed intake, and feed conversion ratio.

[0040] In a further preferred embodiment of the present invention, improving the egg production performance of laying hens includes increasing the egg production rate, increasing the average egg weight, and reducing the feed conversion ratio.

[0041] In this embodiment, the egg production performance was measured as follows: Egg production, egg weight, and the number and weight of deformed eggs were recorded and tallied daily in replicates. Feed intake was also periodically tallied, and the average daily feed intake, egg production rate, average egg weight, and feed conversion ratio were calculated based on the experimental records. Through the above measurement methods, the improvement effect of the present invention's egg-laying hen feed on the mid-to-late stages of egg production performance can be evaluated.

[0042] In a further preferred embodiment of the present invention, the improvement of egg quality includes increasing at least one of the following: yolk index, albumen height, Haugh unit, egg shape index, and eggshell thickness.

[0043] In this embodiment, the egg quality is determined as follows: eggs are collected at set intervals, and the average egg weight, egg shape index, yolk index, yolk ratio, albumen height, Haugh unit, eggshell strength, eggshell thickness, and eggshell ratio are measured. Eggshell thickness can be obtained by removing the inner and outer shell membranes and measuring the thickness at the large end, small end, and middle of the eggshell, then averaging the measurements. Albumen height, Haugh unit, and yolk color can be determined using egg quality testing equipment. Through these methods, the improvement effect of the laying hen feed of this invention on the internal quality and eggshell quality of eggs can be evaluated.

[0044] In a further preferred embodiment of the present invention, Figure 1 This is a schematic diagram illustrating the preparation and application process of the laying hen feed containing ornithine-aspartic acid active additives according to the present invention. Figure 2 This is a schematic diagram of the composition of the laying hen feed containing ornithine-aspartic acid active additive of the present invention.

[0045] In this embodiment, by Figure 1 This invention can demonstrate the complete process from raw material preparation, basal diet mixing, graded premixing, secondary mixing, feeding application to effect evaluation; through Figure 2 It can demonstrate the compositional relationships between corn-soybean meal basal diets, ornithine-aspartic acid active additives, basal diet nutrient levels, and various raw material categories, enabling those skilled in the art to complete feed formulation, additive mixing, and application in laying hens based on the contents of the instruction manual, thus meeting the requirement of sufficient disclosure.

[0046] Experimental Example 1: Experimental Design in Disclosure Materials

[0047] To verify the application effect of this invention, 315 healthy 37-week-old Lohmann pink laying hens of similar weight from the same batch were randomly divided into 3 treatment groups, with 7 replicates in each group and 15 hens in each replicate. The experimental groups are as follows:

[0048] The negative control group, designated as the NC group, was fed a corn-soybean meal basal diet.

[0049] The positive control group, designated as PC group, was supplemented with 250 mg / kg ornithine and 250 mg / kg aspartic acid separately in the basal diet;

[0050] The experimental group, designated as the OA group, had 500 mg / kg of ornithine-aspartic acid active additive added to their basal diet.

[0051] The experiment lasted 14 weeks, including a 2-week pre-feeding period and a 12-week formal trial. The formal trial was conducted with interim testing in 4-week intervals. All treatment groups received isonitrogenous and isoenergetic diets.

[0052] Table 1. Composition and Components of Basal Feed

[0053] corn 60.98 soybean meal 22.89 Corn protein powder 2.78 soybean oil 1.02 wheat bran 0.98 L-Lysine hydrochloride 0.12 Calcium hydrogen phosphate 1.42 stone powder 4.42 Gravel 4.42 choline chloride 0.14 DL-methionine 0.13 antioxidants 0.03 salt 0.35 Vitamin premix 0.03 Trace element premix 0.20 alanine 0.10 total 100.00 Metabolic energy 2.73 Mcal / kg crude protein 16.76% calcium 3.87% Total phosphorus 0.65% Available phosphorus 0.39% Lysine 0.75% Methionine 0.38%

[0054] Methionine 0.38%

[0055] Table 2. Effects of dietary supplementation with ornithine, aspartic acid, and OA on egg production performance of laying hens at different stages.

[0056] 39-41 W Egg production rate % 90.61±1.89 93.04±1.04 95.00±0.89 0.102 39-41 W Average daily feed intake (g) 101.41±2.34 103.40±2.50 104.11±1.57 0.668 39-41 W Average egg weight (g) 59.41±0.35 59.47±0.36 60.32±0.56 0.279 39-41 W FCR 1.87±0.06 1.85±0.05 1.82±0.03 0.729 41-43 W Egg production rate % 87.30±1.42 89.34±1.64 88.78±1.12 0.584 41-43 W Average daily feed intake (g) 108.30±1.12 109.75±1.27 109.61±1.70 0.721 41-43 W Average egg weight (g) 59.44±0.53 59.81±0.43 60.96±0.53 0.115 41-43 W FCR 1.97±0.04 1.99±0.06 1.90±0.03 0.277 43-45 W Egg production rate % 84.07±1.22 87.36±1.39 87.00±1.75 0.253 43-45 W Average daily feed intake (g) 106.52±1.66 108.59±1.27 107.97±2.24 0.703 43-45 W Average egg weight (g) 60.20±0.51 60.94±0.47 61.75±0.55 0.134 43-45 W FCR 2.00±0.02 1.95±0.05 1.89±0.04 0.163 45-47 W Egg production rate % 85.95±2.18 90.12±1.42 91.97±1.10 0.054 45-47 W Average daily feed intake (g) 111.05±1.59 111.05±1.51 112.59±0.97 0.668 45-47 W Average egg weight (g) 60.35±0.54 60.73±0.30 62.39±0.39 0.008 45-47 W FCR 2.13±0.07 1.99±0.05 1.82±0.06 0.010 47-49 W Egg production rate % 88.10±1.24 90.16±2.52 93.51±1.61 0.154 47-49 W Average daily feed intake (g) 112.85±2.08 113.02±1.31 113.22±1.30 0.986 47-49 W Average egg weight (g) 60.60±0.50 61.08±0.37 62.97±0.53 0.007 47-49 W FCR 2.07±0.05 2.00±0.03 1.89±0.06 0.042 49-51 W Egg production rate % 88.58±0.55 91.11±1.02 93.24±1.30 0.017 49-51 W Average daily feed intake (g) 121.42±3.44 118.85±1.83 121.33±0.67 0.671 49-51 W Average egg weight (g) 60.89±0.40 61.19±0.55 62.26±0.63 0.202 49-51 W FCR 2.06±0.07 1.88±0.06 1.85±0.04 0.046 39-51 W Egg production rate % 87.87±0.62 90.52±1.13 92.67±0.61 0.003 39-51 W Average daily feed intake (g) 116.01±2.27 115.01±1.25 116.47±0.27 0.783 39-51 W Average egg weight (g) 60.62±0.35 60.99±0.39 62.32±0.51 0.030 39-51 W FCR 2.02±0.02 1.94±0.04 1.86±0.02 0.006

[0057] Table 2 shows that, compared with the basal diet control group, the OA group improved the laying rate and average egg weight of laying hens during the 39-51 week period and reduced the feed conversion ratio. Compared with the PC group supplemented with 250 mg / kg ornithine and 250 mg / kg aspartic acid alone, the 500 mg / kg OA group showed better performance in terms of average egg weight throughout the 45-47 week, 47-49 week, and 39-51 week periods, indicating that adding ornithine and aspartic acid in the form of ornithine-aspartic acid active additives can achieve better improvement in laying performance under the same total supplementation amount.

[0058] Table 3. Effects of dietary supplementation with ornithine, aspartic acid, and OA on egg quality at different stages of laying hens.

[0059] 43 W Average egg weight (g) 60.03±0.57 60.28±0.59 61.15±0.44 0.331 43 W Egg-shaped index 1.30±0.00 1.31±0.00 1.31±0.01 0.034 43 W Egg yolk index 0.39±0.00 0.39±0.00 0.40±0.00 0.009 43 W Egg yolk ratio % 27.91±0.11 28.07±0.27 28.31±0.27 0.488 43 W Protein height (mm) 7.60±0.26 7.72±0.11 7.97±0.19 0.427 43 W Haas unit 87.05±1.40 87.70±0.66 88.87±1.08 0.505 43 W Eggshell strength (kgf) 5.05±0.07 5.15±0.08 5.19±0.03 0.330 43 W Eggshell thickness (mm) 0.39±0.01 0.40±0.01 0.42±0.01 0.094 43 W Eggshell percentage 13.33±0.04 13.29±0.08 13.40±0.12 0.674 47 W Average egg weight (g) 63.67±0.49 63.87±0.46 65.88±0.66 0.021 47 W Egg-shaped index 1.29±0.01 1.30±0.00 1.31±0.00 0.029 47 W Egg yolk index 0.42±0.01 0.43±0.01 0.42±0.01 0.253 47 W Egg yolk ratio % 26.95±0.08 27.00±0.21 27.31±0.07 0.169 47 W Protein height (mm) 7.52±0.18 8.52±0.29 8.85±0.43 0.026 47 W Haas unit 85.47±1.15 91.18±1.51 92.25±2.21 0.026 47 W Eggshell strength (kgf) 5.26±0.07 5.35±0.11 5.52±0.09 0.199 47 W Eggshell thickness (mm) 0.34±0.01 0.34±0.01 0.35±0.01 0.732 47 W Eggshell percentage 12.74±0.07 12.65±0.14 12.75±0.14 0.832 51 W Average egg weight (g) 60.27±0.40 61.55±0.56 62.48±0.62 0.032 51 W Egg-shaped index 1.29±0.01 1.31±0.01 1.31±0.01 0.091 51 W Egg yolk index 0.42±0.01 0.41±0.01 0.42±0.01 0.603 51 W Egg yolk ratio % 28.91±0.22 29.36±0.22 28.77±0.23 0.187 51 W Protein height (mm) 7.27±0.07 7.55±0.11 7.68±0.07 0.012 51 W Haas unit 84.98±0.50 86.32±0.81 86.95±0.44 0.095 51 W Eggshell strength (kgf) 5.34±0.04 5.42±0.15 5.47±0.02 0.589 51 W Eggshell thickness (mm) 0.30±0.01 0.30±0.00 0.33±0.01 ＜0.001 51 W Eggshell percentage 13.76±0.14 13.52±0.09 13.58±0.07 0.274

[0060] Table 3 shows that the OA group improved the yolk index of 43-week eggs, the average egg weight, albumen height, and Haugh unit of 47-week eggs, and the average egg weight, albumen height, and shell thickness of 51-week eggs. These results demonstrate that the present invention can not only improve the egg production performance of laying hens in the mid-to-late stages of egg production, but also improve the internal quality of eggs and the quality of eggshells.

[0061] Experimental conclusions: Based on the combined results of egg production performance and egg quality, it is evident that under corn-soybean meal basal diet conditions, the addition of ornithine and aspartic acid alone, as well as the addition of ornithine-aspartic acid active additive, can improve egg production performance and egg quality at various stages of laying hens to varying degrees. However, under the same total addition amount, compared with the addition of 250 mg / kg ornithine and 250 mg / kg aspartic acid alone, the addition of 500 mg / kg ornithine-aspartic acid active additive to the basal diet has a relatively better effect on the production of 39-51 W Lohmann laying hens, simultaneously improving both egg production performance and egg quality. Therefore, this invention has good production application value and clear technical effect support.

[0062] In summary, when using this invention, the raw materials are first prepared according to the formula of a corn-soybean meal type basal diet, including corn, soybean meal, corn gluten meal, soybean oil, wheat bran, dicalcium phosphate, limestone powder, limestone granules, choline chloride, L-lysine hydrochloride, DL-methionine, alanine, antioxidants, salt, vitamin premix, and trace element premix. The above raw materials are mixed in a set ratio to form a corn-soybean meal type basal diet, which can provide the energy, protein, calcium and phosphorus minerals, essential amino acids, vitamins and trace elements required by laying hens in the middle and late stages of egg production, providing basic nutritional conditions for the continuous egg production of laying hens.

[0063] After the basal diet is formulated, ornithine-aspartic acid active additive is weighed at an addition rate of 450-550 mg / kg, preferably 500 mg / kg. Since ornithine-aspartic acid active additive is a low-dose additive, if it is added directly to the entire basal diet, uneven distribution may occur. Therefore, this invention adopts a graded premixing method for addition. Specifically, the ornithine-aspartic acid active additive is first mixed with a portion of the corn-soybean meal basal diet to form a premix in a small amount of basal diet. Then, the premix is ​​added to the remaining corn-soybean meal basal diet for a second mixing to ensure that the ornithine-aspartic acid active additive is evenly dispersed in the complete layer hen feed.

[0064] The prepared laying hen feed containing ornithine-aspartic acid active additives is used for continuous feeding of laying hens after 37 weeks of age, preferably for 39-51 week old Lohmann Powder laying hens. During feeding, laying hens can eat and drink freely. The energy and protein raw materials in the basal diet provide basic nutrition for laying hens to maintain life activities, protein deposition and egg formation. Calcium and phosphorus supplements are used to meet the needs of eggshell formation. Amino acid supplements, vitamin premix and trace element premix are used to maintain the normal nutritional metabolism of laying hens in the middle and late stages of laying.

[0065] After ornithine-aspartic acid active additives are ingested by laying hens with feed, the ornithine and aspartic acid structural units in them participate in the regulation of amino acid metabolism, nitrogen metabolism and liver nutritional metabolism. Due to continuous egg production, laying hens in the middle and late stages of egg production have a greater burden on liver lipid synthesis, yolk formation and nutrient transport, which can easily lead to a decline in egg production performance and a deterioration in egg quality. This invention adds ornithine and aspartic acid to the diet in the form of pre-prepared active additives, so that the two have better synchronicity and stability during feed mixing and body ingestion, thereby helping to improve the nutritional utilization status of laying hens in the middle and late stages of egg production.

[0066] Through continuous feeding, this invention can improve the laying rate and average egg weight of laying hens and reduce the feed conversion ratio without changing the conventional corn-soybean meal basal diet system. At the same time, it improves egg quality indicators such as yolk index, albumen height, Haugh unit, egg shape index and eggshell thickness. Compared with the separate addition of ornithine and aspartic acid, this invention uses ornithine-aspartic acid active additive as a whole additive, which can achieve more stable laying performance and egg quality improvement under the same total addition amount, thereby achieving the goal of improving laying hen production efficiency and commercial egg quality.

[0067] It is worth noting that the circuits, electronic components, and modules involved in this invention are all existing technologies, which can be fully implemented by those skilled in the art, and need not be elaborated upon. The content protected by this invention does not involve improvements to the software and methods.

[0068] It should be understood that the disclosed apparatus can be implemented in other ways, given the several embodiments provided in this application. For example, the apparatus embodiments described above are merely illustrative; the division of units described above is only a logical functional division, and in actual implementation, there may be other division methods. For example, multiple units or components may be combined or integrated into another system, or some features may be ignored or not executed. Furthermore, the coupling or communication connections shown or discussed may be through some interfaces; the indirect coupling or communication connections between devices or units may be telecommunications or other forms.

[0069] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit the scope of protection of the invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on these embodiments, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art can still combine, add, delete, or otherwise adjust the features of the various embodiments of the present invention according to the circumstances without conflict or creative effort, thereby obtaining different technical solutions that do not fundamentally depart from the concept of the present invention. These technical solutions also fall within the scope of protection of the present invention.

Claims

1. A layer hen feed containing ornithine-aspartic acid active additive, characterized in that, The diet includes a corn-soybean meal basal diet and an ornithine-aspartic acid active additive added to the corn-soybean meal basal diet. The ornithine-aspartic acid active additive is a pre-prepared additive containing ornithine and aspartic acid structural units. The amount of the ornithine-aspartic acid active additive added to the laying hen feed is 450-550 mg / kg. The laying hen feed is used for continuous feeding of laying hens after 37 weeks of age.

2. The laying hen feed containing ornithine-aspartic acid active additive according to claim 1, characterized in that, The amount of the ornithine-aspartic acid active additive added to the laying hen feed is 500 mg / kg.

3. The laying hen feed containing ornithine-aspartic acid active additive according to claim 1, characterized in that, The corn-soybean meal basal diet includes energy feed, protein feed, calcium and phosphorus supplement feed, amino acid supplement feed, vitamin premix, and trace element premix.

4. The laying hen feed containing ornithine-aspartic acid active additive according to claim 3, characterized in that, The energy source includes corn, soybean oil, and wheat bran; the protein source includes soybean meal and corn gluten meal; the calcium and phosphorus supplements include dicalcium phosphate, limestone powder, and limestone granules; and the amino acid supplements include L-lysine hydrochloride, DL-methionine, and alanine.

5. The laying hen feed containing ornithine-aspartic acid active additive according to claim 4, characterized in that, Based on 100 parts by weight of the corn-soybean meal basal diet, the corn-soybean meal basal diet includes 60.98 parts corn, 22.89 parts soybean meal, 2.78 parts corn gluten meal, 1.02 parts soybean oil, 0.98 parts wheat bran, 0.12 parts L-lysine hydrochloride, 1.42 parts dicalcium phosphate, 4.42 parts limestone powder, 4.42 parts limestone granules, 0.14 parts choline chloride, 0.13 parts DL-methionine, 0.03 parts antioxidant, 0.35 parts salt, 0.03 parts vitamin premix, 0.20 parts trace element premix, and 0.10 parts alanine.

6. The laying hen feed containing ornithine-aspartic acid active additive according to claim 1, characterized in that, The calculated nutrient levels of the corn-soybean meal basal diet include metabolizable energy 2.70-2.76 Mcal / kg, crude protein 16.50%-17.00%, calcium 3.70%-4.00%, total phosphorus 0.60%-0.70%, available phosphorus 0.35%-0.45%, lysine 0.70%-0.80%, and methionine 0.34%-0.42%.

7. The laying hen feed containing ornithine-aspartic acid active additive according to claim 6, characterized in that, The calculated nutrient levels of the corn-soybean meal basal diet include metabolizable energy 2.73 Mcal / kg, crude protein 16.76%, calcium 3.87%, total phosphorus 0.65%, available phosphorus 0.39%, lysine 0.75%, and methionine 0.38%.

8. The laying hen feed containing ornithine-aspartic acid active additive according to claim 1, characterized in that, The ornithine-aspartic acid active additive is added to the corn-soybean meal basal diet through a graded premixing method. The graded premixing method includes first mixing the ornithine-aspartic acid active additive with a portion of the corn-soybean meal basal diet to form a premix, and then mixing the premix with the remaining corn-soybean meal basal diet.

9. The application of the ornithine-aspartic acid active additive in laying hen feed according to any one of claims 1-8 in improving the egg production performance of laying hens, characterized in that, The laying hens are Lohmann pink laying hens aged 37 weeks and older, and the laying hen feed is continuously fed during the 39-51 week age period. The improvement of laying hen egg production performance includes increasing the laying rate, increasing the average egg weight, and reducing the feed conversion ratio.

10. The application of the ornithine-aspartic acid active additive in laying hen feed according to any one of claims 1-8 in improving egg quality, characterized in that, The laying hens are Lohmann Pink laying hens aged 37 weeks or older, and the improvement of egg quality includes increasing at least one of the following: yolk index, albumen height, Haugh unit, egg shape index, and eggshell thickness.

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