Preparation method of clear-type polygonatum odoratum mother liquor

Abstract

The application discloses a preparation method of a clarified polygonatum odoratum original juice, and comprises the following steps: (1) beating or wet grinding; (2) slurry juice separation; (3) heat treatment induced flocculation; (4) natural sedimentation separation; (5) clear juice separation; (6) removal of additional moisture; and (7) sterilization and filling: the polygonatum odoratum original juice obtained in the step (6) is subjected to sterilization, filling and cooling treatment to prepare a clarified polygonatum odoratum original juice product. The method realizes efficient clarification of the polygonatum odoratum slurry juice through heat treatment induced flocculation, has the advantages of simple process, low cost, no introduction of exogenous additives, high product stability, long shelf life and the like, and can provide high-quality raw materials for deep processing.

Description

Technical Field

[0001] This invention belongs to the field of agricultural product processing technology, and specifically relates to a method for preparing clarified Solomon's Seal juice. Background Technology

[0002] Polygonatum odoratum, a perennial herb belonging to the genus Polygonatum in the family Liliaceae, has rhizomes rich in polysaccharides, saponins, flavonoids, and other active ingredients. It possesses properties such as nourishing yin and moistening dryness, promoting body fluid production and quenching thirst, and is a traditional food and medicine homology substance. With the development of the health industry, the demand for Polygonatum odoratum as a functional food ingredient is increasing, and processed products such as beverages and concentrated liquids made from it are gradually gaining market attention.

[0003] Currently, processed products of Solomon's Seal are mainly primary raw materials such as dried slices and dried strips, with relatively few deep-processed products. Juice is an important raw material for liquid foods, and its production is a crucial link in the development of the deep-processing industry for plant-based agricultural products, especially fruits and vegetables. The clarity of plant-based beverages not only affects the product's appearance but is also a key factor in product quality and shelf-life stability. Therefore, clarified products hold an absolute dominant position in the plant-based beverage market, leading to a strong demand for clarified juice. Common methods for clarifying plant-based juice include ultrafiltration, enzymatic hydrolysis, or the addition of clarifying agents, which present problems such as high investment costs, damage to juice components, or the introduction of exogenous substances.

[0004] A search revealed several patent reports concerning methods for preparing Polygonatum odoratum juice. For example, an invention patent titled "Polygonatum odoratum Juice" (application number CN200710192499.0) describes a process using Polygonatum odoratum fruit embryos as raw material. After washing, the embryos are sequentially pulverized to 120 mesh, 200 mesh, and finally 600 mesh to form a paste. The paste is then soaked in water and heated, followed by separation of the pulp and residue to extract the juice. Finally, the juice is clarified by physical heating at 100-110℃ to obtain a clear juice. The core of this process involves multi-stage ultrafine pulverization combined with high-temperature (100-110℃) clarification. This process is energy-intensive, and the ultrafine pulverization process may cause the material temperature to rise, affecting the stability of heat-sensitive active ingredients. Furthermore, the pulverization degree reaches a maximum of 600 mesh, which may make it difficult for some small particles to settle. The final product is a blended Polygonatum odoratum beverage, rather than the original Polygonatum odoratum juice used as a raw material for further processing.

[0005] Another patent application, CN201010512566.4, entitled "A Production Method for Beauty and Health Functional Beverages Made Using Polygonatum odoratum," describes a technical solution where fresh Polygonatum odoratum is pulverized and mixed with 2 parts safflower and 68 parts purified water. The mixture is heated to 98°C for pre-cooking, then cooled to 65-70°C, and amylase is added for enzymatic hydrolysis. This technical solution introduces other raw materials such as safflower and uses amylase for enzymatic hydrolysis, which raises the possibility of residual exogenous additives. The process is relatively complex and may also damage certain components in the system.

[0006] In summary, current technologies lack a simple process that requires no exogenous additives and yields high clarity and stable quality in the preparation of Polygonatum odoratum juice. Developing a simple, efficient method for preparing Polygonatum odoratum juice without introducing exogenous additives is of great significance for promoting the standardization and high-quality development of the Polygonatum odoratum deep processing industry. Summary of the Invention

[0007] The purpose of this invention is to provide a method for preparing clarified Polygonatum odoratum juice. This method achieves efficient clarification of Polygonatum odoratum juice by inducing flocculation through heat treatment. The process is simple, low-cost, and does not introduce exogenous additives.

[0008] The above-mentioned objective of this invention can be achieved through the following technical solution: a method for preparing clarified Polygonatum odoratum juice, comprising the following steps:

[0009] (1) Pulping or wet grinding: Mix the pretreated fresh Solomon's Seal with water and then pulp or wet grind to obtain Solomon's Seal pulp;

[0010] (2) Separation of pulp and juice: The raw pulp of Solomon's seal obtained in step (1) is pressed and filtered to obtain Solomon's seal juice;

[0011] (3) Heat treatment to induce flocculation: Heat the Polygonatum odoratum slurry obtained in step (2) to eliminate foam and induce the formation of flocs in the slurry;

[0012] (4) Natural sedimentation separation: Let the Polygonatum odoratum juice that has formed obvious flocs in step (3) stand and clarify, so that the flocs settle naturally;

[0013] (5) Separation of clear juice: When the flocculated precipitate and the clear juice are clearly separated, separate the clear juice and the flocculated precipitate;

[0014] (6) Remove added water: The clear juice obtained in step (5) is concentrated in a conventional manner to remove the added water in step (1) and obtain clear Solomon's Seal juice;

[0015] (7) Sterilization and filling: The Solomon's Seal juice obtained in step (6) is sterilized, filled and cooled to obtain a clear Solomon's Seal juice product.

[0016] In the above-mentioned method for preparing clarified Polygonatum odoratum juice:

[0017] Preferably, the pretreatment in step (1) includes one or more of the following: removal of roots and impurities, routine washing, and slicing.

[0018] Preferably, the water in step (1) is purified water, and the mass ratio of the Solomon's seal to the purified water is 1:0.75~2.5.

[0019] More preferably, the water in step (1) is purified water, and the mass ratio of the Solomon's seal to the purified water is 1:1.5.

[0020] Preferably, the pulping or wet grinding in step (1) is carried out using a high-speed blender, wet pulverizer, grinder or pulping machine; the particle size of the Solomon's seal pulp is controlled between 40 mesh and 100 mesh.

[0021] More preferably, the particle size of the Solomon's Seal pulp in step (1) is controlled at 80 mesh.

[0022] Preferably, in step (2), an 80-300 mesh filter or filter cloth is used for filtration.

[0023] More preferably, a 100-mesh filter or filter cloth is used for filtration in step (2).

[0024] Preferably, in step (3), the obtained Solomon's Seal slurry is heated to 60~100℃ to eliminate foam, induce the formation of flocculents in the slurry, and keep warm until the foam on its surface completely disappears.

[0025] More preferably, in step (3), the obtained Solomon's Seal slurry is heated to 80~95°C to eliminate foam, induce the formation of flocculents in the slurry, and keep warm until the foam on its surface completely disappears.

[0026] In some embodiments of the present invention, in step (3), the obtained Solomon's Seal slurry is heated to 95°C to eliminate foam, induce the formation of flocculents in the slurry, and keep warm until the foam on its surface completely disappears. Then, it is packaged, sealed, and taken out by boiling water bath.

[0027] Preferably, the settling and clarification in step (4) is carried out in a sealed stainless steel container with an air filter valve.

[0028] More preferably, the settling and clarification described in step (4) is carried out in a sealed 45° conical bottom stainless steel tank with an air filter valve.

[0029] Preferably, in step (4), the clarification is allowed to stand until the location of the flocculated precipitate is below 1 / 3 of the total volume of the Solomon's seal slurry.

[0030] More preferably, in step (4), the clarification is allowed to stand until the flocculated precipitate is located at 1 / 4 to 1 / 5 of the total volume of the Solomon's Seal slurry.

[0031] Preferably, in step (5), when separating the clear juice and flocculated precipitate, the supernatant is discharged by siphon or through an outlet higher than the position of the flocculated precipitate to obtain clear juice; the flocculated precipitate is separated by centrifugation, and the juice obtained by centrifugation is heated again to induce the formation of residual flocs and allowed to stand for clarification, and the supernatant is combined into the clear juice.

[0032] Preferably, the centrifuge speed is 3000~10000 rpm during centrifugation.

[0033] Ideally, the centrifuge speed is 8000 rpm during centrifugation.

[0034] In some embodiments of the present invention, the flocculated portion remaining in step (5) is centrifuged using a horizontal screw centrifuge.

[0035] Preferably, the conventional concentration in step (6) is conventional low-temperature vacuum concentration.

[0036] Compared with the prior art, the present invention has the following beneficial effects:

[0037] (1) Good clarification effect, no need to add exogenous substances: This invention induces the natural polysaccharides, proteins and other components in the Solomon's Seal to flocculate by heating the Solomon's Seal. The Solomon's Seal with high clarity can be obtained by natural sedimentation and centrifugation. There is no need to add exogenous clarifying agents such as pectinase and gelatin, thus avoiding the residue of exogenous substances and maintaining the natural properties of the product.

[0038] (2) Simple process and low cost: The process of this invention is simple, requiring only conventional unit operations such as crushing, pulping, filtration, heat treatment, sedimentation, centrifugation and sterilization. The equipment requirements are low, making it suitable for industrial production and significantly reducing production costs.

[0039] (3) High product stability: Through heat treatment to induce flocculation, colloidal particles and macromolecular substances that cause turbidity in the later stage are effectively removed, and the stability of the obtained Solomon's seal juice is significantly improved.

[0040] (4) Provide high-quality raw materials for deep processing: The original juice of Solomon's seal prepared by this invention has high clarity and stable quality. It can be used as a high-quality bulk raw material for deep processing products such as Solomon's seal beverages, Solomon's seal concentrate, and Solomon's seal functional foods, and has good prospects for industrial application. Attached Figure Description

[0041] The present invention will now be further described with reference to the accompanying drawings and embodiments.

[0042] Figure 1The process flow diagrams are for the preparation methods of clarified Polygonatum odoratum juice provided in Examples 1-4 of this invention;

[0043] Figure 2 The Polygonatum odoratum juice obtained by pressing and filtering in Example 1 of this invention;

[0044] Figure 3 This refers to the Polygonatum odoratum juice after standing and clarifying in Example 1 of the present invention;

[0045] Figure 4 This refers to the Polygonatum odoratum Shangqing formula in Embodiment 1 of the present invention. Detailed Implementation

[0046] The following embodiments are for illustrative purposes only and do not limit the scope of protection of the present invention. Those skilled in the art can achieve the objectives of the present invention based on the above-disclosed scope.

[0047] Unless otherwise specified, the following implementation schemes generally follow standard testing conditions or the testing conditions recommended by the instrument company. Unless otherwise specified, all materials and reagents used are commercially available. Unless otherwise specified, the methods used below are conventional methods in the field, such as crushing, pulping, filtering, heating, centrifugation, sterilization, filling, and cooling.

[0048] The present invention will be further described below through specific embodiments, but the scope of protection of the present invention is not limited thereto.

[0049] Example 1

[0050] like Figure 1 As shown, the preparation method of clarified Polygonatum odoratum juice provided in this embodiment includes the following steps:

[0051] Select 5 kg of fresh Solomon's Seal (Polygonatum odoratum) harvested in autumn after three years of cultivation. Remove roots and impurities, wash as usual, and cut into slices about 0.3 cm thick. Mix with 7.5 kg of purified water and blend in a household high-speed blender until a 60-80 mesh pulp is obtained. Filter through a 100-mesh nylon filter cloth to obtain Solomon's Seal juice. Figure 2 As shown.

[0052] Heat it in a water bath to 85-90℃ to induce floc formation, keep it warm until the foam on its surface completely disappears, then dispense it into glass bottles with caps, tighten the caps, and then take it out after boiling in a water bath for 10-15 minutes.

[0053] After leaving it to stand overnight, as Figure 3 As shown.

[0054] The supernatant was siphoned, and the remaining flocculent portion was centrifuged (4000 rpm, 15 min) to separate the juice and precipitate. The separated juice was then reheated to induce the formation of residual flocculants. After settling, the supernatant was siphoned again, and the two supernatants were combined, weighing 9.88 kg. Figure 4 As shown.

[0055] The transmittance (T720) of the Polygonatum odoratum supernatant was 92% (the transmittance at a wavelength of 720nm was measured using a spectrophotometer, the same below), the soluble solids content was 10.3°Brix (measured using a saccharimeter, the same below), and the pH (pH meter) was 5.30.

[0056] After removing added water through conventional low-temperature vacuum concentration, 4.51 kg of Solomon's Seal juice was obtained, with the soluble solids content increasing to 22.6°Brix and the light transmittance (T720) reaching 90.2%. Further processing, including sterilization, filling, and cooling, yielded clarified Solomon's Seal juice.

[0057] Example 2

[0058] like Figure 1 As shown, the preparation method of clarified Polygonatum odoratum juice provided in this embodiment includes the following steps:

[0059] Select 5kg of fresh Solomon's Seal that has been cultivated for three years and harvested in autumn. Remove the roots and impurities, wash it as usual, and cut it into slices about 0.3cm thick. Mix it with 5kg of purified water and blend it into a 60-80 mesh pulp using a household blender. Then, press and filter it through a 100-mesh nylon filter cloth to obtain Solomon's Seal juice.

[0060] Heat it in a water bath to 85-90℃ to induce floc formation, keep it warm until the foam on its surface completely disappears, then dispense it into glass bottles with caps, tighten the caps, and then take it out after boiling in a water bath for 10-15 minutes.

[0061] Let it stand overnight.

[0062] The supernatant was siphoned, and the remaining flocculated portion was centrifuged (4000 rpm, 15 min) to separate the juice and precipitate. The separated juice was reheated after centrifugation to induce the formation of residual flocculation. After standing, the supernatant was siphoned again, and the supernatants from the two siphoning were combined, weighing 7.40 kg.

[0063] The test results showed that the transmittance (T720) of the Polygonatum odoratum supernatant was 91.5%, the soluble solids content was 13.5°Brix, and the pH was 5.25.

[0064] After removing added water through conventional low-temperature vacuum concentration, 4.54 kg of Solomon's Seal juice was obtained, with the soluble solids content rising to 22.0°Brix and the light transmittance (T720) reaching 90.2%. Further processing, including sterilization, filling, and cooling, yielded clarified Solomon's Seal juice.

[0065] Example 3

[0066] like Figure 1 As shown, the preparation method of clarified Polygonatum odoratum juice provided in this embodiment includes the following steps:

[0067] Select 5kg of fresh Solomon's Seal that has been cultivated for three years and harvested in autumn. Remove the roots and impurities, wash it as usual, and cut it into slices about 0.3cm thick. Mix it with 10kg of purified water and blend it into a 60-80 mesh pulp using a household blender. Then, press and filter it through a 100-mesh nylon filter cloth to obtain Solomon's Seal juice.

[0068] Heat it in a water bath to 85-90℃ to induce floc formation, keep it warm until the foam on its surface completely disappears, then dispense it into glass bottles with caps, tighten the caps, and then take it out after boiling in a water bath for 10-15 minutes.

[0069] Let it stand overnight.

[0070] The supernatant was siphoned, and the remaining flocculated portion was centrifuged to separate the juice and precipitate. The separated juice was then heated again to induce the formation of residual flocculation. After settling, the supernatant was siphoned again, and the two supernatants were combined, weighing 12.25 kg.

[0071] The test results showed that the transmittance (T720) of the supernatant of Polygonatum odoratum was 95%, the soluble solids content was 8.4°Brix, and the pH was 5.35.

[0072] After removing added water through conventional low-temperature vacuum concentration, 4.39 kg of Solomon's Seal juice was obtained, with the soluble solids content increasing to 23.4°Brix and the light transmittance (T720) reaching 89.4%. Further processing, including sterilization, filling, and cooling, yielded clarified Solomon's Seal juice.

[0073] Example 4

[0074] like Figure 1 As shown, the preparation method of clarified Polygonatum odoratum juice provided in this embodiment includes the following steps:

[0075] Select 500 kg of fresh Solomon's Seal that has been cultivated for three years and harvested in autumn. Remove the roots and impurities, wash it as usual, cut it into slices about 0.3 cm thick, mix it with 750 kg of purified water, and grind it into a pulp of 60-80 mesh using a wet pulverizer. Then press and filter it through a 100-mesh stainless steel mesh to obtain Solomon's Seal juice.

[0076] The material is heated to 90°C with steam from an extraction tank and circulated intermittently to induce floc formation. It is then kept warm until the surface foam completely disappears and pumped into a sterilized conical-bottom storage tank equipped with a filter valve.

[0077] Let it stand overnight.

[0078] The supernatant is discharged from the valve located above the flocculated material. The remaining flocculated material is centrifuged in a horizontal screw centrifuge to separate the juice and precipitate. The separated juice is reheated to induce the formation of residual flocculation. After settling, the supernatant is discharged again. The two supernatants are combined, weighing 996 kg.

[0079] The test results showed that the transmittance (T720) of the Polygonatum odoratum supernatant was 92.2%, the soluble solids content was 10.4°Brix, and the pH was 5.30.

[0080] After removing added water through conventional low-temperature vacuum concentration, 461 kg of Solomon's Seal juice was obtained, with the soluble solids content rising to 22.5°Brix and the light transmittance (T720) reaching 89.9%. Further processing, including sterilization, filling, and cooling, yielded clarified Solomon's Seal juice.

[0081] The above embodiments are merely examples illustrating the technical solutions of the present invention and are not intended to limit the invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions claimed by the present invention.

Claims

1. A method for preparing clarified Polygonatum odoratum juice, characterized in that, Includes the following steps: (1) Pulping or wet grinding: Mix the pretreated fresh Solomon's Seal with water and then pulp or wet grind to obtain Solomon's Seal pulp; (2) Separation of pulp and juice: The raw pulp of Solomon's seal obtained in step (1) is pressed and filtered to obtain Solomon's seal juice; (3) Heat treatment to induce flocculation: Heat the Polygonatum odoratum slurry obtained in step (2) to eliminate foam and induce the formation of flocs in the slurry; (4) Natural sedimentation separation: Let the Polygonatum odoratum juice that has formed obvious flocs in step (3) stand and clarify, so that the flocs settle naturally; (5) Separation of clear juice: When the flocculated precipitate and the clear juice are clearly separated, separate the clear juice and the flocculated precipitate; (6) Remove added water: The clear juice obtained in step (5) is concentrated in a conventional manner to remove the added water in step (1) and obtain clear Solomon's Seal juice; (7) Sterilization and filling: The Solomon's Seal juice obtained in step (6) is sterilized, filled and cooled to obtain a clear Solomon's Seal juice product.

2. The method for preparing clarified Polygonatum odoratum juice according to claim 1, characterized in that, The pretreatment described in step (1) includes one or more of the following: removal of roots, impurities, routine washing, and slicing.

3. The method for preparing clarified Polygonatum odoratum juice according to claim 1, characterized in that, The water mentioned in step (1) is purified water, and the mass ratio of the Solomon's Seal to the purified water is 1:0.75~2.

5.

4. The method for preparing clarified Polygonatum odoratum juice according to claim 1, characterized in that, The pulping or wet grinding in step (1) is carried out using a high-speed blender, wet pulverizer, grinder or pulping machine; the particle size of the Solomon's seal pulp is controlled between 40 mesh and 100 mesh.

5. The method for preparing clarified Polygonatum odoratum juice according to claim 1, characterized in that, In step (2), an 80-300 mesh filter or filter cloth is used for filtration.

6. The method for preparing clarified Polygonatum odoratum juice according to claim 1, characterized in that, In step (3), the obtained Solomon's Seal slurry is heated to 60~100℃ to eliminate foam, induce the formation of flocculents in the slurry, and keep warm until the foam on its surface completely disappears.

7. The method for preparing clarified Polygonatum odoratum juice according to claim 1, characterized in that, The settling and clarification described in step (4) is carried out in a sealed stainless steel container with an air filter valve.

8. The method for preparing clarified Polygonatum odoratum juice according to claim 1, characterized in that, In step (4), the sedimentation is allowed to stand until the flocculated precipitate is located at less than 1 / 3 of the total volume of the Solomon's Seal slurry.

9. The method for preparing clarified Polygonatum odoratum juice according to claim 1, characterized in that, In step (5), when separating the clear juice and flocculated precipitate, the supernatant is discharged by siphon or through an outlet higher than the position of the flocculated precipitate to obtain clear juice; the flocculated precipitate is separated by centrifugation, and the juice obtained by centrifugation is heated again to induce the formation of residual flocs and allowed to stand for clarification, and the supernatant is combined into the clear juice.

10. The method for preparing clarified Polygonatum odoratum juice according to claim 9, characterized in that, The centrifuge speed is 3000~10000 rpm during centrifugation.

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