A compound enzymolysis method suitable for spleen-invigorating food materials and application thereof

Abstract

The application discloses a kind of compound enzymatic hydrolysis method suitable for spleen-strengthening food material and application thereof, and belongs to the technical field of enzymatic hydrolysis and food with medicinal value processing.The application mixes and grinds common spleen-strengthening food with medicinal value and special compound enzyme, sieves and then adds water to carry out twice enzymatic hydrolysis, and then inactivates enzyme, reduces pressure and concentrates to obtain compound enzymatic hydrolysis product;The compound enzymatic hydrolysis product is mixed with refined honey to make honeyed pill.The application customizes core special compound enzyme according to the common components of common spleen-strengthening food material.The application breaks cell wall first, then degrades macromolecular polysaccharide, and finally refines to maximize release functional components under mild conditions.Non-active original polysaccharide is converted into functional spleen-strengthening active polysaccharide by enzymatic hydrolysis.The method of the application can be applied to all common spleen-strengthening food materials in pharmacopoeia / food with medicinal value directory, and is universal and compatible.

Description

Technical Field

[0001] This invention relates to the field of enzymatic hydrolysis and food processing technology, specifically to a compound enzymatic hydrolysis method applicable to spleen-strengthening ingredients and its application. Background Technology

[0002] Spleen-strengthening medicinal and edible ingredients are the core raw materials for light fasting, meal replacements, and gastrointestinal conditioning foods. Polysaccharides are their common functional substances. Current processing mainly relies on physical grinding and boiling. However, the original polysaccharides are in a large, inactive molecular state, resulting in extremely low dissolution and utilization rates. Existing traditional processes only involve grinding; the natural polysaccharides in spleen-strengthening medicinal and edible ingredients are large molecules, leading to low dissolution, poor absorption, and weak functional activity. Furthermore, existing meal replacements lack universal enzymatic hydrolysis technology, only suitable for single formulas, and cannot cover all types of spleen-strengthening ingredients. Even if enzymatic hydrolysis methods exist, they are only single-enzyme hydrolysis, and single enzymes cannot degrade the common cell walls and large-molecule polysaccharides of spleen-strengthening ingredients, resulting in extremely low yields of functional spleen-strengthening polysaccharides. These problems lead to product homogenization, an inability to quantify effects with objective components, a heavy digestive burden on large-molecule components, and an inability to meet the sustained satiety needs of light fasting meal replacements.

[0003] Therefore, providing a compound enzymatic hydrolysis method suitable for spleen-strengthening foods, which can break down the foods into small molecules and achieve a stable satiety effect, is an urgent problem to be solved in this field. Summary of the Invention

[0004] In view of this, the present invention provides a compound enzymatic hydrolysis method and its application applicable to spleen-strengthening food ingredients. It belongs to a general compound enzyme applicable to common spleen-strengthening food and medicinal ingredients, which can directionally convert inactive native polysaccharides into spleen-strengthening active polysaccharides.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] A compound enzymatic hydrolysis method suitable for spleen-strengthening ingredients includes the following steps: (1) Weigh out common spleen-strengthening food ingredients, mix them with a special compound enzyme, and then crush and sieve them; The special compound enzyme is composed of the following raw materials in parts by weight: 10-30 parts cellulase, 10-30 parts pectinase, 10-30 parts β-glucanase, 5-20 parts neutral protease, 3-15 parts tanninase, 3-15 parts phytase, 3-15 parts polyphenol oxidase, and 3-15 parts melibiase. (2) Add water to the obtained materials and mix them evenly to obtain a slurry; (3) The obtained slurry was subjected to a first enzymatic hydrolysis at 45-55℃ and pH 4.5-5.2; (4) The slurry after the first enzymatic hydrolysis is subjected to a second enzymatic hydrolysis at 35-50℃ and pH 5.2-7.0; (5) The slurry after the second enzymatic hydrolysis was inactivated and concentrated under reduced pressure to obtain a thick paste-like enzymatic hydrolysis product; (6) Dry the thick paste thoroughly, then pulverize it into fine, residue-free enzymatic hydrolyzed small molecule food powder. Mix the obtained powder with refined honey to make honey pills.

[0007] In this invention, spleen-strengthening dietary ingredients refer to the traditional spleen-strengthening dietary ingredients classified as such in "Traditional Chinese Medicine Diet Therapy" (a textbook for the 14th Five-Year Plan of Traditional Chinese Medicine Colleges and Universities) and "Dietary Therapy Materia Medica," including food ingredients that are both food and medicine, as well as common foods such as millet and black beans.

[0008] Functional polysaccharides are derived from spleen-strengthening foods and are water-soluble heteropolysaccharides obtained through enzymatic hydrolysis. They possess high water solubility, high water-holding capacity, and high swelling capacity, making them active factors that enhance satiety. They have the following characteristics: ① Structure: The main chain is β-D-glucan, and the side chains contain glucose, mannose, galactose, arabinose, and rhamnose, forming neutral or acidic heteropolysaccharides. ② Physicochemical properties: High water solubility, high water-holding capacity, and high swelling capacity; soluble in water to form a stable colloid.

[0009] The active polysaccharide formed in this invention is a mixture of oligosaccharides and small molecule polysaccharides prepared from spleen-strengthening food ingredients that are both food and medicine, according to the above-mentioned method. Its molecular formula conforms to: ; Its structural formula conforms to: ; In the formula, n = 2~46, corresponding to a molecular weight range of 342 Da to 8000 Da. The main chain is composed of α-D-glucopyranose linked by α-(1→4) glycosidic bonds, and does not contain monosaccharide components, which is a typical active small molecule polysaccharide structure.

[0010] Furthermore, the common spleen-strengthening medicinal and edible ingredients mentioned in step (1) are any one or a mixture of Polygonatum sibiricum, ginseng, licorice, black beans, jujubes, Poria cocos, yam, Euryale ferox, coix seed, lotus seeds and dried tangerine peel.

[0011] Preferably, its composition includes the following raw materials in parts by weight: 5-30 parts of Polygonatum sibiricum, 5-50 parts of ginseng, 5-15 parts of licorice, 10-50 parts of black beans, 10-50 parts of jujubes, 10-50 parts of Poria cocos, and 30-35 parts of dried tangerine peel.

[0012] Furthermore, the mass ratio of the commonly used spleen-strengthening medicinal and edible ingredients to the special compound enzyme is 1:0.003-0.008.

[0013] Furthermore, the sieving in step (1) is sieving through a 60-80 mesh sieve.

[0014] Furthermore, in step (2), the ratio of the screened material to the water is 1:6-12.

[0015] Furthermore, the first enzymatic hydrolysis time in step (3) is 120-180 min; the second enzymatic hydrolysis time in step (4) is 150-210 min.

[0016] Furthermore, the enzyme inactivation described in step (5) is performed at 75-85℃ for 20 minutes; The vacuum concentration is carried out at 65-75°C to concentrate the solids to 35%-55%.

[0017] Furthermore, the complex enzymatic hydrolysis product obtained in step (5) of this invention can be made into product forms such as honey pills, powders, and ointments.

[0018] Furthermore, in step (6), the mass ratio of the compound enzymatic hydrolysis product to the refined honey is 1:0.8-1.2, and the diameter of the honey pill is 9mm.

[0019] This invention also provides the application of the general compound enzymatic hydrolysis product prepared by the above method, suitable for spleen-strengthening ingredients, in light fasting meal replacements.

[0020] This invention utilizes compound enzymatic hydrolysis to directionally degrade macromolecular polysaccharides, coarse fibers, and hard particles in food into small-molecule active polysaccharides, eliminating the root cause of "roughness, infusibility, and residue" from a structural perspective. The enzymatic hydrolysis is completed in an aqueous phase, ensuring thorough hydrolysis. Subsequent concentration, drying, and ultrafine pulverization yield an extremely fine enzymatically hydrolyzed powder. The small-molecule active polysaccharides produced by enzymatic hydrolysis fully interact with refined honey to form a homogeneous polysaccharide-honey slow-release complex. This complex exhibits slow swelling and gradual melting characteristics at oral temperature, ensuring both easy absorption and avoiding excessively rapid absorption, thus achieving a slow-release absorption.

[0021] The beneficial effects of this invention are as follows: This invention customizes a core-specific compound enzyme targeting the common components of common spleen-strengthening foods. This invention first breaks down cell walls, then degrades macromolecular polysaccharides, and finally refines them, maximizing the release of functional components under gentle conditions. Through enzymatic hydrolysis, inactive native polysaccharides are converted into functional spleen-strengthening active polysaccharides. The method of this invention is applicable to all common spleen-strengthening foods listed in the pharmacopoeia / food and medicine homology catalogue, and is universally compatible. Attached Figure Description

[0022] Figure 1 This is a comparison image of the experimental group and the control group after adding 1 ml of artificial saliva in Experiment Example 3 of the present invention; Figure 2 This is a comparison image of the experimental group and the control group after adding 2ml of artificial saliva in Experiment Example 3 of the present invention. Detailed Implementation

[0023] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0024] Example 1: A compound enzymatic hydrolysis method suitable for spleen-strengthening ingredients (1) Weigh out common spleen-strengthening food ingredients, mix them with a special compound enzyme, and then pulverize them through an 80-mesh sieve; The special compound enzyme consists of: 20 parts cellulase, 20 parts pectinase, 20 parts β-glucanase, 12 parts neutral protease, 8 parts tanninase, 8 parts phytase, 10 parts polyphenol oxidase, and 10 parts melibiase. Common ingredients for strengthening the spleen and being derived from both food and medicine are: 20 parts Polygonatum sibiricum, 35 parts ginseng, 12 parts licorice, 35 parts black beans, 25 parts jujubes, 30 parts Poria cocos, and 30 parts dried tangerine peel. The mass ratio of common spleen-strengthening medicinal and edible ingredients to specialized compound enzymes is 1:0.005. (2) Add water to the obtained materials and stir evenly to obtain a slurry. The ratio of material to water is 1:10. (3) The obtained slurry was enzymatically hydrolyzed at 50℃ and pH 4.8 for 160 min; (4) The slurry after the first enzymatic hydrolysis was enzymatically hydrolyzed for 180 min at 40℃ and pH 6.5; (5) The slurry after the second enzymatic hydrolysis was inactivated at 85°C for 20 min and concentrated under reduced pressure at 65°C to 45% solids to obtain a thick paste-like enzymatic hydrolysis product. (6) The thick paste is thoroughly dried and then ultra-finely pulverized to obtain a fine, residue-free enzymatic hydrolyzed small molecule food powder. The obtained powder is mixed with refined honey at a mass ratio of 1:1 to make honey pills with a mass of 9g.

[0025] Example 2: A compound enzymatic hydrolysis method suitable for spleen-strengthening ingredients (1) Weigh out common spleen-strengthening food ingredients, mix them with a special compound enzyme, and then pulverize them through an 80-mesh sieve; The special compound enzyme consists of: 10 parts cellulase, 10 parts pectinase, 30 parts β-glucanase, 20 parts neutral protease, 3 parts tanninase, 3 parts phytase, 15 parts polyphenol oxidase, and 15 parts melibiase. Common ingredients for strengthening the spleen and being derived from both food and medicine are: 20 parts Polygonatum sibiricum, 35 parts ginseng, 12 parts licorice, 35 parts black beans, 25 parts jujubes, 30 parts Poria cocos, and 30 parts dried tangerine peel. The mass ratio of common spleen-strengthening medicinal and edible ingredients to special compound enzymes is 1:0.008; (2) Add water to the obtained materials and stir evenly to obtain a slurry. The ratio of material to water is 1:8. (3) The obtained slurry was enzymatically hydrolyzed at 45℃ and pH 4.5 for 120 min; (4) The slurry after the first enzymatic hydrolysis was enzymatically hydrolyzed for 150 min at 35℃ and pH 5.2; (5) The enzyme inactivation solution after the second enzymatic hydrolysis was carried out at 85°C for 20 min and concentrated under reduced pressure at 65°C to 55% solids to obtain a thick paste-like enzymatic hydrolysis product. (6) The thick paste is thoroughly dried and then ultra-finely pulverized to obtain a fine and residue-free enzymatic hydrolyzed small molecule food powder. The obtained powder is mixed with refined honey at a mass ratio of 1:1.2 to make honey pills with a mass of 9g.

[0026] Example 3: A compound enzymatic hydrolysis method suitable for spleen-strengthening ingredients (1) Weigh out common spleen-strengthening food ingredients, mix them with a special compound enzyme, and then pulverize them through an 80-mesh sieve; The special compound enzyme consists of: 30 parts cellulase, 30 parts pectinase, 10 parts β-glucanase, 5 parts neutral protease, 15 parts tanninase, 15 parts phytase, 3 parts polyphenol oxidase, and 3 parts melibiase. Common ingredients for strengthening the spleen and being derived from both food and medicine are: 20 parts Polygonatum sibiricum, 35 parts ginseng, 12 parts licorice, 35 parts black beans, 25 parts jujubes, 30 parts Poria cocos, and 30 parts dried tangerine peel. The mass ratio of common spleen-strengthening medicinal and edible ingredients to special compound enzymes is 1:0.003; (2) Add water to the obtained materials and stir evenly to obtain a slurry. The ratio of material to water is 1:12. (3) The obtained slurry was enzymatically hydrolyzed at 55℃ and pH 5.2 for 180 min; (4) The slurry after the first enzymatic hydrolysis was enzymatically hydrolyzed at 50℃ and pH 7.0 for 210 min; (5) The slurry after the second enzymatic hydrolysis was inactivated at 75°C for 20 min and concentrated under reduced pressure at 65°C to 35% solids to obtain a thick paste-like enzymatic hydrolysis product. (6) The thick paste is thoroughly dried and then ultra-finely pulverized to obtain a fine and residue-free enzymatic hydrolyzed small molecule food powder. The obtained powder is mixed with refined honey at a mass ratio of 1:0.8 to make honey pills with a mass of 9g.

[0027] Experiment Example 1: Effect Verification In vitro physicochemical testing and verification of the effects of consumption by healthy individuals have confirmed that the general compound enzymatic hydrolysis process, compared with the traditional pulverization process, can meet the needs of sustained satiety and saliva production in light fasting meal replacements.

[0028] ① The yield of spleen-strengthening active polysaccharides was significantly improved. According to the phenol-sulfuric acid method, the extraction rate of spleen-strengthening active polysaccharides in the spleen-strengthening food and medicine raw materials after enzymatic hydrolysis in Example 1 increased by 3.2 times, and the proportion of water-soluble polysaccharides increased from 28% to 92%, indicating a clear enrichment effect of functional components.

[0029] ②Polysaccharide molecular weight direction optimization According to high-performance gel filtration chromatography, the molecular weight of polysaccharides in traditional processes is mainly distributed in the range of 10kDa–100kDa. The methods in Examples 1-3 of this invention can directionally degrade them into small-molecule spleen-strengthening active polysaccharides of 1kDa–5kDa, which greatly improves bioavailability and absorption efficiency.

[0030] ③ Enhanced core physical properties of meal replacement According to the weighing method, the enzymatic hydrolysis product of Example 1 has a 2.8-fold increase in swelling power and a 2.3-fold increase in water retention. It can form a stable hydrogel in the gastrointestinal tract, providing physical support for a long-lasting feeling of fullness, and is perfectly suited for the application scenario of light fasting meal replacement.

[0031] ④ Excellent health benefits for healthy individuals A non-clinical trial was conducted on 24 healthy volunteers. The results showed that a feeling of fullness was produced within 15 minutes after consumption, and the feeling of fullness could last for 4-6 hours. The gastrointestinal tract was not burdened, and the tolerance was good, which could effectively meet the consumption needs of light fasting and meal replacement. Table 1 shows the average changes in body composition and dimensions of the volunteers after one week of fasting.

[0032] Table 1

[0033] Experimental Example 2: Laboratory Verification of Chewing Performance Experimental groups: Test group: the large honey pills prepared in Example 1 of this invention; Control group: the same formula large honey pills that were only crushed and not enzymatically hydrolyzed.

[0034] Test basis and methods: The physical environment of the oral cavity at a constant temperature of 37°C and artificial saliva immersion was simulated. The standard methods for food physicochemical testing were used to objectively evaluate quantifiable indicators such as melting, dissolution, residue, and particle size of the honey pills, proving that they have the physical basis for ingestion.

[0035] Test parameters and instruments: Dissolution time test: Instrument: 37℃ constant temperature water bath; Method: Place the honey pill in artificial saliva and observe the time for complete dissolution / melting.

[0036] Determination of water-soluble residue rate: After the honey pills are completely dissolved, they are filtered, dried, weighed, and the proportion of insoluble residue is calculated.

[0037] Powder particle size distribution detection: The D90 particle size (the diameter of 90% of the particles) was determined using a laser particle size analyzer.

[0038] Laboratory objective test results Dissolution time: The test group completely melted in 5-10 minutes; the control group did not dissolve in more than 30 minutes; the shorter the dissolution time, the easier it is to dissolve in the mouth.

[0039] Insoluble residue rate: ≤1% for the test group; ≥15% for the control group; the lower the residue rate, the better the residue-free properties.

[0040] Powder D90 particle size: test group ≤38μm; control group ≥150μm; the smaller the particle size, the higher the fineness and the easier it is to melt and contain.

[0041] Experimental Example 3: Solubility Test According to the scheme of Example 1, spleen-strengthening ingredients were ground into powder, passed through a 100-mesh sieve, and then processed into honey pills using a compound enzymatic hydrolysis process, which served as the experimental group. The control group was directly made into honey pills.

[0042] Equal mass samples were taken from each group, and 1 ml of artificial saliva was added to each sample on a 37°C operating plate. The dissolution was observed after 5 minutes. The experimental group showed uniform dissolution, while the control group showed granular and uneven dissolution. Figure 1 As shown. Then, 2 ml of artificial saliva was added to each sample, and the dissolution was observed after 15 minutes. It was observed that the experimental group showed better dissolution. Figure 2 As shown.

[0043] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention.

Claims

1. A compound enzymatic hydrolysis method suitable for spleen-strengthening ingredients, characterized in that, Includes the following steps: (1) Weigh out common spleen-strengthening food ingredients, mix them with a special compound enzyme, and then crush and sieve them; The special compound enzyme is composed of the following raw materials in parts by weight: 10-30 parts cellulase, 10-30 parts pectinase, 10-30 parts β-glucanase, 5-20 parts neutral protease, 3-15 parts tanninase, 3-15 parts phytase, 3-15 parts polyphenol oxidase, and 3-15 parts melibiase. (2) Add water to the obtained materials and mix them evenly to obtain a slurry; (3) The obtained slurry was subjected to a first enzymatic hydrolysis at 45-55℃ and pH 4.5-5.2; (4) The slurry after the first enzymatic hydrolysis is subjected to a second enzymatic hydrolysis at 35-50℃ and pH 5.2-7.0; (5) The slurry after the second enzymatic hydrolysis was inactivated and then concentrated under reduced pressure to obtain a thick paste-like enzymatic hydrolysis product; (6) Dry the thick paste thoroughly, then pulverize it into fine, residue-free enzymatic hydrolyzed small molecule food powder. Mix the obtained powder with refined honey to make honey pills.

2. The compound enzymatic hydrolysis method for spleen-strengthening ingredients according to claim 1, characterized in that, The common spleen-strengthening medicinal and edible ingredients mentioned in step (1) are any one or a mixture of Polygonatum sibiricum, ginseng, licorice, black beans, jujube, Poria cocos and dried tangerine peel.

3. A compound enzymatic hydrolysis method for spleen-strengthening food ingredients according to claim 1 or 2, characterized in that, The mass ratio of the commonly used spleen-strengthening medicinal and edible ingredients to the special compound enzyme is 1:0.003-0.

008.

4. The compound enzymatic hydrolysis method for spleen-strengthening ingredients according to claim 1, characterized in that, The sieving mentioned in step (1) is sieving through an 80-mesh sieve.

5. The compound enzymatic hydrolysis method for spleen-strengthening ingredients according to claim 1, characterized in that, In step (2), the ratio of the sieved material to the water is 1:8-12.

6. The preparation method of a universal compound enzymatic hydrolysis applicable to spleen-strengthening food ingredients according to claim 1, characterized in that, The first enzymatic hydrolysis time in step (3) is 120-180 min; the second enzymatic hydrolysis time in step (4) is 150-210 min.

7. The preparation method of a universal compound enzymatic hydrolysis applicable to spleen-strengthening food ingredients according to claim 1, characterized in that, The enzyme inactivation described in step (5) is performed at 75-85℃ for 20 minutes; The vacuum concentration is carried out at 65-75°C to concentrate the solids to 35%-55%.

8. The preparation method of a universal compound enzymatic hydrolysis applicable to spleen-strengthening food ingredients according to claim 1, characterized in that, In step (6), the mass ratio of the compound enzymatic hydrolysis product to the refined honey is 1:0.8-1.2, and the mass of the honey pill is 9g.

9. The application of a universal compound enzymatic hydrolysis product prepared by the method of any one of claims 1-8, suitable for spleen-strengthening ingredients, in a light fasting meal replacement.

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