A dietetic composition with improved immunity and its preparation method

Abstract

The present application relates to the technical field of functional food, in particular to a medicine-food homologous composition with improved immunity and a preparation method thereof. The composition comprises Cistanche, Rhizoma Polygonati, Dendrobium officinale and mulberry. The composition provided by the present application is medicine-food homologous substance, has mild effect, high safety, and the characteristics of warming and moistening, regulating yin and yang, tonifying without dryness, and nourishing without greasiness. The composition can improve the immunity of people with deficiency of vital qi and congenital deficiency, and improve the immunity.

Description

Technical Field

[0001] This invention relates to the field of functional food technology, and in particular to a food-medicine homology composition that enhances immunity and its preparation method. Background Technology

[0002] Sub-health is a common phenomenon among modern people, mainly manifested as physical weakness, lethargy, poor appetite, and sleep disorders. Although it does not meet the diagnostic criteria for a disease, being in this state for a long time will affect bodily functions, increase the risk of various diseases, and reduce quality of life. Immunity, the body's ability to defend against external pathogens and maintain homeostasis, is closely related to sub-health. The immune system function of sub-healthy individuals is often unbalanced, unable to effectively perform its defensive and regulatory functions, thus leading to persistent and difficult-to-improve sub-health symptoms.

[0003] While the concept of "sub-health" doesn't exist in traditional Chinese medicine (TCM) theory, the symptoms of sub-health, such as fatigue, lethargy, and functional disorders, closely align with the TCM concept of "deficiency of vital energy and imbalance of Yin and Yang." TCM believes that human health depends on "balance of Yin and Yang and sufficient vital energy." When vital energy is deficient and Yin and Yang are imbalanced, the body experiences a series of discomforts, including lethargy and fatigue, which corresponds perfectly to the modern medical definition of sub-health. The TCM principle of "strengthening the body's resistance and consolidating its foundation, harmonizing Yin and Yang" is precisely the approach to improving sub-health. By regulating organ function, replenishing vital energy, and harmonizing Yin and Yang, sub-health symptoms can be alleviated at their root. Summary of the Invention

[0004] To address the aforementioned issues, this invention provides a medicinal and edible homologous composition for enhancing immunity and its preparation method. The composition provided by this invention consists entirely of medicinal and edible homologous substances, with mild effects and no toxic side effects. It also possesses the characteristics of warming and nourishing essence, harmonizing yin and yang, qi and blood, and being nourishing without causing dryness or greasiness. For individuals with deficient vital energy or congenital insufficiency, it can improve the body's immune function and enhance immunity.

[0005] To achieve the above objectives, the present invention provides the following technical solution: This invention provides a food-medicine composition that enhances immunity, including Cistanche deserticola, Polygonatum sibiricum, Dendrobium officinale, and mulberry.

[0006] Preferably, the amount of each raw material in the composition is 10g of Cistanche deserticola, 10g of Polygonatum sibiricum, 2g of Dendrobium officinale, and 8g of mulberry.

[0007] The present invention also provides a method for preparing the composition described in the above technical solution, comprising the following steps: 1) Soak the Cistanche deserticola, Polygonatum sibiricum, Dendrobium officinale, and mulberry in water to obtain a soaking material; 2) Boil the soaking material obtained in step 1), filter and concentrate the resulting decoction, and dry it to make a dry powder to obtain the composition.

[0008] Preferably, the total mass ratio of Cistanche deserticola, Polygonatum sibiricum, Dendrobium officinale, and mulberry to water in step 1) is 1:10.

[0009] Preferably, the soaking time in step 1) is 2 hours.

[0010] Preferably, the conditions for decocting in step 2) include: decocting twice, for 30 minutes each time.

[0011] Preferably, the drying conditions in step 2) include: combining the decoctions obtained from two decoctions and filtering them, concentrating the filtrate to obtain an extract, and drying the extract at 60°C to a constant weight to obtain the composition.

[0012] The present invention also provides the application of the composition described in the above technical solution in the preparation of products that enhance immunity.

[0013] The present invention also provides a composition for enhancing immunity, comprising the composition described above and food-acceptable excipients.

[0014] Preferably, the dosage form of the composition includes decoction, granules, pills or capsules.

[0015] Cistanche deserticola is sweet, salty, and warm, tonifying kidney yang and replenishing essence and blood, warming without causing dryness; Polygonatum sibiricum is sweet and neutral, tonifying qi and nourishing yin, strengthening the spleen, moistening the lungs, and benefiting the kidneys, tonifying the three jiaos. Both tonify yin and yang, jointly replenishing kidney essence, making them the principal herbs. Dendrobium nobile is sweet and slightly cold, benefiting the stomach, generating fluids, nourishing yin, and clearing heat. It assists Polygonatum sibiricum in nourishing kidney yin and moistening the lungs and stomach, thus aiding in the postnatal transformation, making it the assistant herb. Mulberry is sweet, sour, and cold, nourishing yin and replenishing blood, generating fluids and moistening dryness. It enters the heart, liver, and kidney meridians, replenishing liver blood and kidney essence. Since essence and blood share the same origin, it enhances the effect of replenishing essence, making it the adjuvant herb. The entire formula is warm and moistening, replenishing essence, harmonizing yin and yang, qi and blood, tonifying without causing dryness, and nourishing without being greasy.

[0016] Beneficial effects: The compositions provided by this invention are all food and medicine homologous substances, with mild effects and no toxic side effects. They also have the characteristics of warming and nourishing essence, regulating yin and yang qi and blood, and being nourishing without being drying and moisturizing without being greasy. For people with deficiency of vital energy and insufficient congenital endowment, they can improve the body's immune function and enhance immunity. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the embodiments will be briefly described below.

[0018] Figure 1Effects of the composition on immune organ indices in immunocompromised mice; A. Spleen index; B. Thymus index; *P<0.05, **P<0.01, compared with the control group; #P<0.05, ##P<0.01, compared with the model group; Figure 2 The effect of the composition on peripheral blood leukocytes in immunocompromised mice; *P<0.05, **P<0.01, compared with the control group; #P<0.05, ##P<0.01, compared with the model group; Figure 3 The effect of the composition on nucleated bone marrow cells in immunocompromised mice; *P<0.05, **P<0.01, compared with the control group; #P<0.05, ##P<0.01, compared with the model group; Figure 4 The effect of the composition on the phagocytic capacity of macrophages in immunocompromised mice; *P<0.05, **P<0.01, compared with the control group; #P<0.05, ##P<0.01, compared with the model group; Figure 5 The effect of the composition on peripheral blood immunoglobulins and cytokines in immunocompromised mice; *P<0.05, **P<0.01, compared with the control group; #P<0.05, ##P<0.01, compared with the model group; Detailed Implementation

[0019] This invention provides a composition for enhancing immunity, comprising Cistanche deserticola, Polygonatum sibiricum, Dendrobium officinale, and Morus alba. In this invention, the dosage of each ingredient in the composition is 10g of Cistanche deserticola, 10g of Polygonatum sibiricum, 2g of Dendrobium officinale, and 8g of Morus alba. The preferred sources of the ingredients are: Polygonatum sibiricum selected from specially cultivated medicinal materials; Dendrobium officinale selected from authentic medicinal materials; and Cistanche deserticola and Morus alba selected from commercially available medicinal materials.

[0020] The present invention also provides a method for preparing the composition described in the above technical solution, comprising the following steps: 1) Soak the Cistanche deserticola, Polygonatum sibiricum, Dendrobium officinale, and mulberry in water to obtain a soaking material; 2) Boil the soaking material obtained in step 1), filter and concentrate the resulting decoction, and dry it to make a dry powder to obtain the composition.

[0021] This invention involves soaking a mixture of Cistanche deserticola, Polygonatum sibiricum, Dendrobium officinale, and mulberry in water to obtain a soaking solution. In this invention, the preferred mass ratio of the total mass of Cistanche deserticola, Polygonatum sibiricum, Dendrobium officinale, and mulberry to water is 1:10. The preferred soaking time is 2 hours.

[0022] In this invention, the obtained soaking material is decocted, the resulting decoction is filtered, concentrated, and then dried to obtain a dry powder, thus yielding a composition. Preferably, the decocting conditions include: decocting twice, 30 minutes each time. Preferably, the drying conditions include: combining the decoctions obtained from the two decoctions, filtering, concentrating the filtrate to obtain an extract, and drying the extract at 60°C to constant weight, thus obtaining the composition.

[0023] The present invention also provides the application of the composition described in the above technical solution in the preparation of products that enhance immunity.

[0024] This invention also provides a composition for enhancing immunity, comprising the composition described above and food-grade excipients. In this invention, the dosage form of the composition preferably includes a decoction, granules, pills, or capsules. This invention does not impose any particular limitation on the preparation method of the above-mentioned decoction, granules, pills, or capsules; conventional preparation methods are acceptable.

[0025] To further illustrate the present invention, the following detailed description is provided in conjunction with embodiments, but these should not be construed as limiting the scope of protection of the present invention.

[0026] Example A food-medicine composition that enhances immunity and its preparation method comprises the following steps: 1) Mix 10g of Cistanche deserticola, 10g of Polygonatum sibiricum, 2g of Dendrobium officinale, and 8g of mulberry with water at a mass ratio of 1:10 and soak for 2 hours to obtain the soaking material; 2) The soaking material obtained in step 1) is decocted, the decoction is filtered and concentrated, and then dried to make a dry powder to obtain the composition; wherein, the decoction is decocted twice, each time for 30 minutes; the decoctions obtained from the two decoctions are combined and filtered, and the filtrate is concentrated by a rotary evaporator to obtain an extract. The extract is placed in a 60°C oven and dried to constant weight to obtain the composition.

[0027] Functional verification test The powdered composition prepared in the examples was mixed with purified water to prepare a solution with a concentration of 180 mg / mL. Cyclophosphamide (CTX) was mixed with physiological saline to prepare a solution with a concentration of 12.5 mg / mL.

[0028] Eighteen healthy male SPF-grade BALB / c mice, weighing 25g and aged 7-8 weeks, were randomly divided into three groups after 3 days of acclimatization. The control group received intraperitoneal injections of the same volume of saline as the model group for 4 consecutive days; the model group received intraperitoneal injections of CTX (50mg / kg) for 4 consecutive days; and the drug group received intraperitoneal injections of CTX (50mg / kg) followed by gavage administration of a drug solution (0.8g / kg) every 4 hours for 4 consecutive days. Starting on day 5, the drug group continued to receive the drug solution via gavage for 28 consecutive days, while the control and model groups received the same volume of purified water via gavage. After the last administration, the mice were fasted overnight for 12 hours. The following morning, the mice were weighed, and after anesthesia, blood was collected from the eyes, and the mice were euthanized by cervical dislocation. The spleen, thymus, and other biological materials were collected for bioassay.

[0029] Indicator Testing: 1. Spleen index and thymus index testing After dissecting the mice, the spleen and thymus were weighed, and the spleen index and thymus index were calculated, which are the ratios of organ weight to body weight.

[0030] 2. Peripheral blood leukocyte count Take 200 μL of whole blood into an EDTA anticoagulant tube, invert to mix, and immediately place into a fully automated blood analyzer for testing.

[0031] 3. Detection of nucleated cell concentration in bone marrow Mouse bone marrow was flushed into EDTA anticoagulant tubes using Hank's solution. 20 μL of bone marrow fluid was transferred to a new centrifuge tube and 0.38 mL of hydrochloric acid was added. The mixture was stirred and 20 μL was transferred to a cell counting chamber for nucleated cell counting.

[0032] Collect whole blood, centrifuge at 3000 r / min for 15 min, and collect serum. Determine the concentrations of IgG, IgM, IFN-γ, and IL-10 in the serum according to the ELISA kit instructions.

[0033] 4. Macrophage phagocytic capacity detection Mice were injected with Indian ink (10 mL / kg) via the tail vein. At 2 and 10 minutes after injection, 20 μL of blood was collected from the orbital cavity and added to 2 mL of 0.1% sodium carbonate solution. After mixing, the absorbance was measured at a wavelength of 600 nm, and the phagocytic index was calculated.

[0034] κ=(lgOD1-lgOD2) / (t2-t1) α=(κ 1 / 3 BM) / (LM+SM) In the formula: κ is the carbon clearance index; α is the phagocytic index; OD1 is the absorbance value at t1 (2 minutes); OD2 is the absorbance value at t2 (10 minutes); BM is the body mass (g); LM is the liver mass (g); SM is the spleen mass (g).

[0035] 5. Detection of peripheral blood immunoglobulins and cytokines Collect whole blood, centrifuge at 3000 r / min for 15 min, and collect serum. Determine the concentrations of IgG, IgM, IFN-γ, and IL-10 in the serum according to the ELISA kit instructions.

[0036] Results and Analysis: (1) Effects of the composition on the immune organ index of immunocompromised mice Figure 1 Data showed that the spleen index A and thymus index B in the Model group were significantly lower than those in the Control group, indicating that CTX caused serious damage to the immune organs of mice and the mouse model of low immunity was successfully established. After intervention with the composition, the spleen index and thymus index of mice both increased significantly, indicating that the composition has a certain alleviating effect on mice with low immunity and increases the body's immune function to a certain extent.

[0037] (2) Effects of the composition on peripheral blood leukocytes in immunocompromised mice according to Figure 2 It is evident that the number of peripheral blood leukocytes in the Model group mice was significantly lower than that in the Control group; the number of peripheral blood leukocytes in the Drug group mice was significantly higher than that in the Model group, and recovered to the level of the Control group, indicating that the composition can improve the peripheral blood leukocyte count level in immunosuppressed mice and enhance immune function.

[0038] (3) Effects of the composition on nucleated cells in the bone marrow of immunocompromised mice Figure 3 Data showed that the number of nucleated cells in the bone marrow of mice in the Model group was significantly lower than that in the Control group. After intervention with the composition, the number of nucleated cells in the bone marrow of mice in the Drug group was significantly improved and was no different from that in the Control group, suggesting that the composition has an ameliorative effect on bone marrow suppression.

[0039] (4) Effect of the composition on the phagocytic capacity of macrophages in immunocompromised mice Figure 4 Data showed that the phagocytic index was significantly lower in the Model group compared to the Control group. Conversely, the phagocytic index was significantly higher in the Drug group compared to the Model group, indicating that the composition enhanced the phagocytic capacity of mouse macrophages.

[0040] (5) Effects of the composition on peripheral blood immunoglobulins and cytokines in immunocompromised mice Figure 5 Data showed that the serum IgG and IgM levels in the Model group were significantly lower than those in the Control group. After intervention with the composition, the IgG and IgM levels significantly rebounded, suggesting that the composition could improve the immune function of mice. The peripheral blood IFN-γ concentration in the Model group was significantly lower than that in the Control group, while the IFN-γ concentration in the Drug group was significantly higher than that in the Model group. Conversely, the peripheral blood IL-10 concentration in the Model group was significantly higher than that in the Control group, while the IL-10 concentration in the Drug group was significantly lower than that in the Model group. These results indicate that the composition has a significant ameliorative effect on immunocompromised mice.

[0041] In summary, the composition can significantly improve the function of immune organs in immunocompromised mice, increase spleen and thymus indices, and repair CTX-induced immune organ damage; increase the number of peripheral blood leukocytes and bone marrow nucleated cells, and improve bone marrow suppression; enhance the phagocytic index of mouse macrophages and strengthen their phagocytic capacity; and regulate the levels of peripheral blood immunoglobulins (IgG, IgM) and cytokines (IFN-γ, IL-10), correcting immune imbalance and thus enhancing the immune function of immunocompromised mice.

[0042] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, and not all embodiments. People can obtain other embodiments based on these embodiments without creative effort, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A food-medicine composition that enhances immunity, characterized in that, Including Cistanche deserticola, Polygonatum sibiricum, Dendrobium officinale, and mulberry.

2. The composition according to claim 1, characterized in that, The dosage of each ingredient in the composition is as follows: 10g of Cistanche deserticola, 10g of Polygonatum sibiricum, 2g of Dendrobium officinale, and 8g of Morus alba.

3. A method for preparing the composition according to claim 1 or 2, characterized in that, Includes the following steps: 1) Soak the Cistanche deserticola, Polygonatum sibiricum, Dendrobium officinale, and mulberry in water to obtain a soaking material; 2) Boil the soaking material obtained in step 1), filter and concentrate the resulting decoction, and dry it to make a dry powder to obtain the composition.

4. The preparation method according to claim 3, characterized in that, In step 1), the total mass ratio of Cistanche deserticola, Polygonatum sibiricum, Dendrobium officinale, and mulberry to water is 1:

10.

5. The preparation method according to claim 3, characterized in that, The soaking time in step 1) is 2 hours.

6. The preparation method according to claim 3, characterized in that, Step 2) The conditions for decocting include: decocting twice, 30 minutes each time.

7. The preparation method according to claim 3, characterized in that, Step 2) The drying conditions include: combining the decoctions obtained from two decoctions and filtering them, concentrating the filtrate to obtain an extract, and drying the extract at 60°C to constant weight to obtain the composition.

8. The use of the composition according to claim 1 or 2 in the preparation of products that enhance immunity.

9. A composition for enhancing immunity, comprising the composition of claim 1 or 2 and a food-acceptable excipient.

10. The composition according to claim 9, characterized in that, The dosage form of the composition includes decoction, granules, pills or capsules.

Images