A constant-temperature immersion device and method for animal experiments on craniocerebral seawater injury

Abstract

The application discloses a kind of cranium brain seawater injury animal experiment thermostatic soaking device and method, it is related to animal experiment equipment technical field, provide a kind of scheme capable of local, controllable soaking to experimental animal skull top wound, the device includes the binding platform for fixing experimental animal body, the limiting table for fixing head, and soaking component is connected on limiting table, soaking component can be moved to cover in animal skull top wound area, and communicate with external water supply component by connecting pipe, using above structure, liquid can be soaked in experimental time only target wound of animal skull top, and the respiratory organ such as mouth, nose of animal remains exposed in air normal breathing, to reduce the risk of water choking or suffocation of animal in living immersion experiment, the structure of the device is convenient for the placement and fixation of experimental animal, relatively simple operation, applicable to simulate the related biomedical experimental research of seawater immersion injury after craniocerebral trauma.

Description

Technical Field

[0001] This invention relates to the field of biomedical engineering technology, and in particular to a constant-temperature immersion device and method for animal experiments involving intracranial seawater injury. Background Technology

[0002] When a person suffers a head injury in an accident at sea (such as a ship collision or falling into the sea), the wound will be directly immersed in seawater. This kind of injury is much more serious than a normal head injury because seawater is more than three times saltier than human body fluids, is more alkaline, and carries various bacteria. Studies have found that after a head injury, immersing the person in cold seawater will cause the body temperature to drop rapidly, which will aggravate brain tissue damage and significantly increase the mortality rate. In animal experimental studies involving the injury mechanism of seawater immersion after traumatic brain injury, it is necessary to immerse the skull wound of experimental animals (such as SD rats) in seawater for a controlled duration to achieve a simulated effect, observe changes in injury, and test the effectiveness of various treatment drugs or methods. A common practice is to fix the animal's body and place its head above a container of experimental saline solution. By adjusting the water level and inverting the animal, the wound area is submerged below the liquid surface. Another practice is to perform tracheal intubation on the animal to maintain breathing, and then immerse the whole body or head.

[0003] The above-mentioned common practices have certain limitations. When adjusting the water level, because the experimental animals are living organisms, they have autonomous breathing movements and may struggle. The relative position of their heads and the liquid surface can easily change slightly, which may cause the animal's mouth and nose to be accidentally submerged, leading to choking or even suffocation. This may result in the unexpected termination of the experiment or the introduction of respiratory system complications. On the other hand, using invasive methods such as tracheal intubation to maintain breathing can cause trauma to the animal's airway and may trigger a local inflammatory response, which brings analytical complexity to distinguishing between seawater immersion injury and operation-related injury. Summary of the Invention

[0004] The purpose of this invention is to address the shortcomings of existing technologies, such as the animal's mouth and nose being easily submerged, leading to choking or even suffocation, and the airway being easily damaged. Therefore, this invention proposes a constant temperature immersion device and method for animal experiments involving intracranial seawater injury.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: A constant temperature immersion device for animal experiments on intracranial seawater injury includes an experimental pool platform, a water storage basin installed on one side of the experimental pool platform, and a wire mesh frame fixedly installed at the bottom of the experimental pool platform. A tying platform is used to limit and fix the body of a laboratory animal. The tying platform includes a tying table and tying straps. A restraint table is used to limit and fix the head of an experimental animal. The restraint table includes a locking platform and a connecting frame. One end of the connecting frame is provided with an immersion tube for covering the injured area. The bottom edge of the immersion tube 317 is provided with a sealing element for sealing contact with the skin on the top of the animal's skull to form a closed immersion cavity. A connector is used to connect the soaking tube to the water storage basin, and the connector has an internal self-opening and closing structure.

[0006] In one possible design, the bottom of the binding platform is provided with a horizontal clamping foot, and the mesh frame is fixedly provided with a main clamping seat and a secondary clamping seat along its length. The side walls of the main clamping seat and the secondary clamping seat are provided with clamping grooves that cooperate with the clamping foot.

[0007] In one possible design, the side wall of the card table has an indentation; The bottom of the card holder is fixedly provided with a limit rod, the binding table is provided with a limit hole corresponding to the limit rod, the top of the mesh frame is fixedly provided with a connecting seat, and the top of the connecting seat is provided with a limit groove corresponding to the limit rod.

[0008] In one possible design, the limiting platform further includes a threaded rod, the top of the main clamp is provided with a threaded groove, and the bottom end of the threaded rod is threaded through the clamp and engages with the threaded groove. Two limiting rings are fixedly provided on the outer wall of the threaded rod along the axial direction. The connecting frame is rotatably sleeved on the outer wall of the smooth rod of the threaded rod and located between the two limiting rings. The connecting frame is slidably arranged along the height direction of the clamping platform. A cavity is opened at the position of the connecting frame corresponding to the soaking cylinder.

[0009] In one possible design, a flexible tube is rotatably provided at the top of the connector, and the other end of the flexible tube is fixedly connected to the water storage basin. The connector is threaded onto the outer wall of the soaking tube. The self-opening and closing structure includes a sealing seat and multiple protrusions arranged in a ring at the top of the soaking cylinder. A fixing frame is fixedly installed inside the connector. The same annular elastic pad is fixedly installed between the sealing seat and the fixing frame. An inner protruding ring is provided on the inner wall of the connector. A boss is provided at the bottom of the sealing seat to seal with the inner protruding ring. The multiple protrusions are located inside the inner circumference of the inner protruding ring and are configured to cooperate with the boss.

[0010] In one possible design, the outer wall of the test tank platform is equipped with a constant temperature heater that works in conjunction with a water storage basin.

[0011] In one possible design, the connecting frame and the soaking tube are detachable.

[0012] A method of using the constant-temperature immersion device for animal experiments involving intracranial seawater injury as described in any one of the claims includes the following steps: S1. Body fixation: The experimental animal's body is placed on a binding table and its chest and abdomen are fixed by binding straps. S2. Platform positioning: Align the clips at the bottom of the binding platform with the slots on the brackets of the space frame, and push the clips in laterally to complete the lateral positioning of the binding platform. S3. Head limiting: The inner notch of the clamping table is engaged with the neck of the experimental animal, and the limiting rod passes through the limiting hole of the binding table and is inserted into the limiting groove of the connecting seat to complete the horizontal limiting of the head. S4. Cylinder mouth sealing: Tighten the threaded rod, and the connecting frame and soaking cylinder will move down through the limiting ring, so that the soaking cylinder covers the area around the wound on the top of the head and seals the scalp. S5. Water Immersion: Screw the connector onto the outer wall of the immersion tube. The protruding end at the top of the immersion tube opens the sealing seat to conduct water. The constant temperature saline in the water storage basin flows into the immersion tube through the hose to immerse the wound at a constant temperature. S6. End Reset: After soaking, unscrew the connector in the opposite direction, reset the sealing seat to cut off the water path, disassemble the restraint platform and the binding platform in sequence, and take out the experimental animal.

[0013] In this application, during actual use, the experimental mouse (SD rat) is first tied to the tying table with a tying strap. Then, the tying table is attached to the mesh frame and moved laterally so that its locking feet are inserted into the corresponding locking slots. Subsequently, the limiting table is inserted downwards onto the tying table, and its bottom limiting rod passes through the limiting hole and is inserted into the limiting groove below to limit the tying table. The locking table will be locked at the neck of the experimental mouse through the concave opening to limit the head. Then, the threaded rod is screwed down to move it downwards. The bottom end of the threaded rod will be screwed into the threaded groove, thereby fixing the locking table and the tying table. The threaded rod is then screwed down to move the connecting frame downwards through the limiting ring. The end of the connecting frame will press down on the head of the experimental mouse, and the soaking tube will cover the area around the injured head of the experimental mouse. Finally, screw the connector onto the outer wall of the soaking tube. When screwing, the convex end at the top of the soaking tube will abut against the sealing seat, thus pushing it upward and away from the inner convex ring. At this time, the prepared saline solution in the water storage basin will flow into the inside of the soaking tube through the hose, thereby soaking the wound on the head of the experimental mouse. After the soaking time is reached, the experimental mouse can be removed using the reverse operation device.

[0014] In this invention, a constant temperature immersion device for animal experiments of intracranial seawater injury is provided. The binding platform and the limiting table are used together to position the animal's body and head respectively. The immersion components can move relative to the limiting table to adapt to different individual animals or experimental requirements. The overall structure facilitates the placement and operation of experimental animals. In this invention, the constant temperature immersion device for animal experiments of intracranial seawater injury has a sealing structure inside the connector that can automatically open or close the water circuit when the connector is connected to or separated from the immersion cylinder. In this invention, during use, an independent soaking component is placed over the wound on the top of the animal's skull and connected to a water supply component, so that the experimental liquid only soaks the target wound area, while the animal's mouth, nose and other respiratory organs remain exposed to the air. This reduces the risk of choking or suffocation caused by accidental submersion of the animal's mouth and nose during live immersion experiments. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the main structure of a constant temperature immersion device for animal experiments involving intracranial seawater injury proposed in this invention; Figure 2 This is a schematic diagram of the exploded right view of the constant temperature immersion device for animal experiments on intracranial seawater injury proposed in this invention; Figure 3 This is a schematic diagram of the exploded left view of the constant temperature immersion device for animal experiments on intracranial seawater injury proposed in this invention; Figure 4 For the present invention Figure 3 Enlarged view of the structure of section A; Figure 5 This is a schematic cross-sectional view of a constant-temperature immersion device for animal experiments involving intracranial seawater injury proposed in this invention. Figure 6 For the present invention Figure 5 Enlarged view of the structure of section B; Figure 7 This is a schematic diagram of the split structure of a constant temperature immersion device for animal experiments involving intracranial seawater injury proposed in this invention.

[0016] In the diagram: 1. Test pool platform; 2. Binding platform; 211. Binding table; 212. Clamping foot; 213. Binding strap; 214. Limiting hole; 3. Limiting table; 311. Clamping table; 312. Threaded rod; 313. Limiting ring; 314. Connecting frame; 315. Limiting rod; 316. Inner notch; 317. Immersion cylinder; 318. Protruding end; 4. Water storage basin; 5. Constant temperature heater; 6. Hose; 7. Connector; 711. Fixing frame; 712. Annular elastic pad; 713. Sealing seat; 714. Inner convex ring; 8. Secondary clamping seat; 9. Main clamping seat; 911. Threaded groove; 10. Clamping groove; 11. Connecting seat; 1111. Limiting groove; 12. Space frame. Detailed Implementation

[0017] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0018] In one embodiment: Reference Figure 1An animal experimental device, the overall structure of which mainly includes: an experimental pool platform 1, a binding platform 2, a restraint platform 3, a water storage basin 4, a constant temperature heater 5, a hose 6, a connector 7, and a wire mesh frame 12.

[0019] The test pool platform 1 is a platform with a surrounding edge and a bottom drainage structure. The mesh frame 12 is fixed to the bottom of the test pool platform 1 with screws. The water storage basin 4 is installed on one side of the test pool platform 1 and is used to hold the experimental prepared brine. The constant temperature heater 5 is installed on the outer wall of the test pool platform 1 and its heating rod extends into the water storage basin 4 to maintain the brine within the set temperature range. The binding platform 2 is used to fix the body of the experimental animal. The restraint platform 3 is used to fix the head of the experimental animal. The connector 7 is used to connect the water storage basin 4 and the soaking component on the restraint platform 3.

[0020] refer to Figure 2 The space frame 12 is welded and fixed with a main card seat 9 and a secondary card seat 8 along its length. The side walls of the main card seat 9 and the secondary card seat 8 are provided with card slots 10. The binding platform 2 includes a rectangular binding table 211. The bottom of the binding table 211 is welded with a horizontally oriented, rectangular-section card foot 212. The top surface of the binding table 211 is provided with a binding strap 213. By placing the binding table 211 on the space frame 12 and aligning the card foot 212 with the card slot 10, the binding table 211 is pushed horizontally to insert the card foot 212 into the card slot 10, thereby achieving the horizontal positioning of the binding platform 2 on the space frame 12.

[0021] refer to Figure 3-4 The limiting platform 3 includes a clamping platform 31. Two limiting rods 315 are vertically welded to the bottom of the clamping platform 311. A limiting hole 214 corresponding to the position of the limiting rod 315 is opened on the binding platform 211. A connecting seat 11 is also welded on the mesh frame 12. A limiting groove 1111 corresponding to the limiting rod 315 is opened on the top of the connecting seat 11. An indentation 316 is opened on the side wall of the clamping platform 311. A threaded rod 312 is installed on one side of the top of the clamping platform 311. Two limiting rings 313 are fixedly sleeved on the smooth part of the threaded rod 312. A connecting frame 314 is installed between the two limiting rings 313. The connecting frame 314 can rotate relative to the threaded rod 312 and can slide along the height direction of the clamping platform 311. A cavity is opened at one end of the connecting frame 314. A cylindrical soaking tube 317 is connected to the cavity. A threaded groove 911 that is threaded to the end of the threaded rod 312 is opened on the top of the main clamping seat 9.

[0022] refer to Figure 5-6The connector 7 is a cylindrical component with open ends. One end is connected to the outer wall of the soaking tube 317 by threads. The sealing seat 713 is supported by a fixed bracket 711 inside the connector 7. The sealing seat 713 and the fixed bracket 711 are connected by an annular silicone elastic pad 712. An integral inner convex ring 714 is provided on the inner wall of the connector 7. In the natural state, the elasticity of the silicone elastic pad 712 causes the protrusion at the bottom of the sealing seat 713 to press tightly into the inner convex ring 714 to form a seal. Multiple protrusions 318 are evenly distributed circumferentially on the outer side of the top of the soaking tube 317. The other end of the connector 7 is connected to a hose 6 through a rotary joint. The other end of the hose is connected to the outlet of the water storage basin 4.

[0023] The working process of the device is as follows: First, place the body of the experimental animal (such as an SD rat) on the soft pad of the binding platform 211, and use the binding strap 213 to wrap around its chest and abdomen for binding and fixation. The force should be such that it restricts its large movement but does not excessively compress its breathing. Then, align the locking foot 212 at the bottom of the binding platform 2 with the locking groove 10 on the mesh frame 12, put it in and push it horizontally until the locking foot 212 is locked in. Then, put the locking platform 311 over the animal's body, so that its inner notch 316 is locked in the neck position of the animal. At the same time, ensure that the two limiting rods 315 pass through the limiting hole 214 on the binding platform 211 and finally insert into the limiting groove 1111 of the lower connecting seat 11 to complete the limitation of the animal's head on the horizontal plane.

[0024] Then, screw the threaded rod 312 down. The threaded rod 312 moves downward and its end gradually screws into the threaded groove 911 of the main seat 9. As the threaded rod 312 continues to screw in, the limiting ring 313 above it will drive the connecting frame 314 to move downward together. The connecting frame 314 presses down, so that the soaking tube 317 at its end will cover the area around the prepared wound on the top of the animal's skull. At this time, the bottom edge of the soaking tube 317 contacts the animal's scalp, forming an isolation area, and a sealing ring is set to prevent subsequent saline leakage. Finally, screw the connector 7 onto the external thread of the soaking cylinder 317. During the screwing process, the protruding end 318 at the top of the soaking cylinder 317 will touch the sealing seat 713 and overcome the elastic force of the silicone elastic pad 712 to push it upward, causing the protrusion of the sealing seat 713 to disengage from the inner protruding ring 714, thereby opening the passage. The constant-temperature saline in the water storage basin 4 flows into the soaking cylinder 317 under the action of gravity through the hose 6 and connector 7, thereby soaking the wound. After soaking, screw the connector 7 off in the opposite direction. In the final stage of unscrewing, the protruding end 318 disengages from the pushing action on the sealing seat 713. Under the action of the elastic force of the silicone elastic pad 712 and the water pressure in the pipe, the sealing seat 713 resets and reseals the inner protruding ring 714, cutting off the water flow. The small amount of water remaining in the soaking cylinder 317 can flow to the drain of the test pool platform through the mesh frame 12 after the animal is removed.

[0025] During the use of this device, it is necessary to regularly clean and lubricate the moving parts such as the threaded rod, groove, and limit rod, and regularly check the aging of the seals and elastic gaskets and replace them in time to ensure the reliability and service life of the device.

[0026] This application can be used in the field of biomedical engineering, or in other fields applicable to this application.

[0027] In another embodiment: Reference Figure 7 A constant temperature immersion device for animal experiments of intracranial seawater injury is applied to the field of biomedical engineering. The structure of this embodiment is basically the same as that of the previous embodiment, except that the connecting frame 314 and the immersion cylinder 317 are detachable structures.

[0028] Its overall structure, assembly relationship and working process are basically the same as those in Example 1. The difference lies in the connection method between the connecting frame 314 and the soaking tube 317. In this example, the end of the connecting frame 314 and the installation position of the soaking tube 317 are separate structures and are fixed by screws. The soaking tube 317 can adopt different inner diameter specifications to adapt to different sizes of cranial wounds or different body sizes of experimental animals.

[0029] The detachable design in this embodiment is more suitable for experimental scenarios that require screening multiple batches of wounds with different specifications.

[0030] However, as is well known to those skilled in the art, the working principle and wiring method of the thermostatic heater 5 are conventional methods or common knowledge, and will not be described in detail here. Those skilled in the art can make any selections according to their needs or convenience.

[0031] The accompanying drawings in this application are for illustrative purposes only. The dimensions and shapes of the components shown are not actual limitations but are merely schematic representations. In actual implementation, the components can be reasonably configured and adjusted according to specific needs and actual conditions.

[0032] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A constant-temperature immersion device for animal experiments involving intracranial seawater injury, characterized in that, include: Test pool platform (1), a water storage basin (4) is installed on one side of the test pool platform (1), and a wire mesh frame (12) is fixedly installed at the bottom of the test pool platform (1). The binding platform (2) is used to limit and fix the body of the experimental animal. The binding platform (2) includes a binding table (211) and binding straps (213). The limiting table (3) is used to limit and fix the head of the experimental animal. The limiting table (3) includes a locking table (311) and a connecting frame (314). One end of the connecting frame (314) is provided with an soaking tube (317) for covering the injured area. The bottom edge of the soaking tube (317) is provided with a sealing element for sealing contact with the skin of the animal's skull to form a closed soaking cavity. A connector (7) is used to connect the soaking tube (317) to the water storage basin (4), and the connector (7) is provided with a self-opening and closing structure inside.

2. The constant-temperature immersion device for animal experiments on intracranial seawater injury according to claim 1, characterized in that, The bottom of the binding platform (2) is provided with a horizontal clamping foot (212). The mesh frame (12) is fixedly provided with a main clamping seat (9) and a secondary clamping seat (8) along the length direction. The side walls of the main clamping seat (9) and the secondary clamping seat (8) are provided with a clamping groove (10) that cooperates with the clamping foot (212).

3. The constant-temperature immersion device for animal experiments on intracranial seawater injury according to claim 2, characterized in that, The side wall of the card holder (311) is provided with an indentation (316). The bottom of the card holder (311) is fixedly provided with a limiting rod (315), the binding table (211) is provided with a limiting hole (214) corresponding to the limiting rod (315), the top of the mesh frame (12) is fixedly provided with a connecting seat (11), and the top of the connecting seat (11) is provided with a limiting groove (1111) corresponding to the limiting rod (315).

4. The constant-temperature immersion device for animal experiments on intracranial seawater injury according to claim 3, characterized in that, The limiting platform (3) also includes a threaded rod (312), and the top of the main card seat (9) is provided with a threaded groove (911). The bottom end of the threaded rod (312) is threaded through the card seat (311) and cooperates with the threaded groove (911). Two limiting rings (313) are fixedly provided on the outer wall of the threaded rod (312) along the axial direction. The connecting frame (314) is rotatably sleeved on the smooth outer wall of the threaded rod (312) and located between the two limiting rings (313). The connecting frame (314) is slidably provided along the height direction of the mounting plate (311). The connecting frame (314) has a cavity hole at the position corresponding to the soaking cylinder (317).

5. The constant-temperature immersion device for animal experiments on intracranial seawater injury according to claim 4, characterized in that, The top end of the connector (7) is rotatably provided with a hose (6), and the other end of the hose (6) is fixedly connected to the water storage basin (4). The connector (7) is threaded onto the outer wall of the soaking cylinder (317). The self-opening and closing structure includes a sealing seat (713) and a plurality of protrusions (318) arranged in a ring at the top of the soaking tube (317). A fixing frame (711) is fixedly arranged inside the connector (7). The same annular elastic pad (712) is fixedly arranged between the sealing seat (713) and the fixing frame (711). An inner protruding ring (714) is provided on the inner wall of the connector (7). A boss is provided at the bottom of the sealing seat (713) to seal with the inner protruding ring (714). The plurality of protrusions (318) are located in the inner circumference of the inner protruding ring (714) and are arranged to cooperate with the boss.

6. The constant-temperature immersion device for animal experiments on intracranial seawater injury according to claim 1, characterized in that, The outer wall of the test pool platform (1) is equipped with a constant temperature heater (5) that works in conjunction with the water storage basin (4).

7. The constant-temperature immersion device for animal experiments involving intracranial seawater injury according to claim 1, characterized in that, The connecting frame (314) and the soaking tube (317) are detachable structures.

8. A method of using a constant-temperature immersion device for animal experiments involving intracranial seawater injury as described in any one of claims 1 to 7, characterized in that, Includes the following steps: S1. Body fixation: The experimental animal's body is placed on the binding table (211) and its chest and abdomen are fixed by binding straps (213). S2, Platform Positioning: Align the clips (212) at the bottom of the binding platform (2) with the slots (10) on the card seat of the grid frame (12), and push the clips (212) in laterally to complete the lateral positioning of the binding platform (2); S3. Head limiting: The inner recess (316) of the clamping platform (311) is clamped onto the neck of the experimental animal, and the limiting rod (315) passes through the limiting hole (214) of the binding platform (211) and is inserted into the limiting groove (1111) of the connecting seat (11) to complete the horizontal limiting of the head. S4. Cylinder sealing: Tighten the threaded rod (312), and the connecting frame (314) and soaking cylinder (317) will move down through the limiting ring (313), so that the soaking cylinder (317) covers the area around the wound on the top of the head and seals the scalp. S5. Soaking in water: Screw the connector (7) onto the outer wall of the soaking tube (317). The protruding end (318) at the top of the soaking tube (317) opens the sealing seat (713) to open the water passage. The constant temperature saline in the water storage basin (4) flows into the soaking tube (317) through the hose (6) to soak the wound at a constant temperature. S6. End Reset: After soaking, unscrew the connector (7) in the opposite direction, reset the sealing seat (713) to cut off the water path, disassemble the limiting platform (3) and the binding platform (2) in sequence, and take out the experimental animal.

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