A device for subcutaneous multi-point injection of mice

Abstract

The present application relates to the technical field of administration apparatus, provide a kind of for mouse subcutaneous multi-point injection device, including injection component, multiple injection needle, positioning component, first adjusting component and administration component, injection component is formed with injection cavity, injection cavity is equipped with liquid inlet and multiple liquid outlet, each liquid outlet is evenly distributed, multiple injection needle is communicated with corresponding liquid outlet respectively, positioning component is used to be fixed on mouse skin around injection area with adsorption, first adjusting component is installed on positioning component, injection component is arranged on adjusting component, to adjust the distance of injection needle tip relative to mouse skin.The present application can solve the problem of repeated puncture and position adjustment in traditional single-needle injection method by multi-point synchronous injection around injection area, reduce operation time, improve the uniformity of drug distribution in tissue, and thus improve the accuracy and repeatability of drug efficacy evaluation.

Description

Technical Field

[0001] This invention relates to the technical field of drug delivery devices, specifically a device for subcutaneous multi-point injection in mice. Background Technology

[0002] In biomedical research, mouse dorsal circular wound models (including subcutaneous xenograft models) are widely used to explore wound healing mechanisms, evaluate the efficacy of local drugs, and validate novel drug delivery systems. Currently, local drug administration to such models is mostly done through topical application. However, due to the skin barrier function and limitations in drug permeability, topical drugs are difficult to effectively penetrate the epidermis and be fully absorbed by the target tissue, resulting in low drug bioavailability and significantly affecting the accuracy and reproducibility of experimental results.

[0003] To improve the local concentration and uniformity of drug distribution in the target area, the industry is gradually shifting towards a strategy of circular multi-point injection around wounds or tumors. According to experimental guidelines, a single administration typically requires at least six injection points in a circular area to ensure uniform drug coverage around the tumor or wound edge. In high-throughput drug efficacy screening scenarios, researchers need to perform repeated procedures on hundreds of mice daily, placing extremely high demands on injection efficiency and standardization.

[0004] However, traditional injection methods rely primarily on a single micro-injection needle and manual operation, which has significant drawbacks. Specifically, single-needle injection can only achieve a single injection point, requiring repeated punctures and point-by-point adjustments, making the operation cumbersome and unsuitable for high-throughput experiments. Furthermore, the needle depth depends entirely on the operator's experience, making it highly susceptible to problems such as injecting too shallowly (leading to drug spillage into the subcutaneous tissue or body surface) or injecting too deeply (causing the needle to penetrate tissue or even damage deep muscles or organs), severely impacting drug administration consistency and experimental data reliability. Therefore, there is an urgent need for an easy-to-use, multi-point synchronous, depth-controllable, and accurately positioned injection device to overcome these shortcomings and improve the standardization, operational efficiency, and experimental reliability of multi-point intratumoral drug administration in mice. Summary of the Invention

[0005] This invention provides a subcutaneous multi-point injection device for mice, which effectively solves the problems of repeated punctures, uneven drug distribution, uncontrollable needle depth, and unstable positioning caused by single-needle injection in the prior art. Specific implementation methods are as follows: A device for subcutaneous multi-point injection in mice includes an injection assembly, wherein the injection assembly forms an injection chamber, the injection chamber is provided with an inlet and multiple outlets, and the outlets are evenly distributed. Multiple sets of injection needles are connected to corresponding liquid outlets. The injection assembly adjusts the injection depth of the injection needles so that the needle tip reaches the target injection area. Positioning components are used to adhere and fix the device to the mouse skin around the injection area; The first adjustment component is mounted on the positioning component, and the injection component is disposed on the adjustment component to adjust the distance between the tip of the injection needle and the mouse skin; The drug delivery assembly includes a drug container and a metering pump. The drug container is connected to the inlet of the injection assembly via a pipeline. The metering pump is located on the pipeline and is used to deliver the drug solution to the injection chamber according to a preset dose, so as to realize multi-needle metered injection.

[0006] As a further embodiment of the present invention, the injection assembly has a cylindrical structure, and multiple sets of injection needles are distributed around the circumference of the cylindrical injection assembly.

[0007] As a further aspect of the present invention, the first adjustment component includes a support base for supporting the injection component; A telescopic rod is connected between the support base and the positioning component to adjust the height of the support base relative to the positioning component; A locking element, disposed on the telescopic rod, is configured to lock the telescopic rod in its telescopic state after adjustment, thereby limiting the initial height of the injection assembly and the injection needle connected thereto relative to the bottom surface of the positioning assembly.

[0008] As a further aspect of the present invention, the injection assembly includes a barrel seat disposed on the first adjustment assembly; The guide cylinder is movably fitted onto the cylinder base; A syringe is movably mounted on a guide cylinder. The syringe has an injection chamber and a piston is provided thereon to push the liquid medicine in the injection chamber through the outlet to the injection needle. The injection needle is installed at the bottom end of the syringe. The second adjustment component connects the syringe and the guide cylinder to adjust the axial position of the syringe relative to the guide cylinder, thereby adjusting the distance between the needle tip of the injection needle and the bottom surface of the syringe base.

[0009] As a further embodiment of the present invention, the guide cylinder is provided with an elastic element, the two ends of which abut against the guide cylinder and the cylinder seat respectively. When the guide cylinder loses the action of external force, the elastic element is configured to drive the guide cylinder to reset.

[0010] As a further embodiment of the present invention, the syringe has a dispensing chamber, one end of which is connected to the injection chamber and the other end is connected to a plurality of outlets. A one-way flow structure is provided in the flow channel between the dispensing chamber and the injection chamber to allow the drug solution to flow unidirectionally from the injection chamber into the dispensing chamber.

[0011] As a further embodiment of the present invention, the unidirectional guiding structure includes a conical guide port and a ball. The conical guide port is formed at the communication channel between the liquid distribution chamber and the liquid injection chamber, with its small diameter end facing the liquid injection chamber. The ball is limited within the conical guide port by a spring.

[0012] As a further embodiment of the present invention, the second adjustment component includes a control rod, the end of which is rotatably connected to a syringe or a guide cylinder. The control rod is provided with a threaded section, and one of the syringe or the guide cylinder is threadedly connected to the threaded section, so as to adjust the axial position of the syringe relative to the guide cylinder by rotating the control rod.

[0013] As a further embodiment of the present invention, the second adjustment component further includes a stop member threadedly connected to the threaded section of the control rod and configured to abut against the syringe or the guide cylinder in a tightened state to restrict axial movement of the syringe relative to the guide cylinder.

[0014] As a further embodiment of the present invention, the positioning component includes an annular base, the bottom surface of which is provided with a plurality of negative pressure suction cups. The negative pressure suction cups are connected to a negative pressure cavity formed in the annular base. The negative pressure cavity is connected to an external negative pressure source through an air passage interface to form an adsorption force at the negative pressure suction cups, thereby detachably fixing the annular base to the mouse skin.

[0015] Due to the adoption of the above technical solutions, the beneficial technical effects of the present invention are as follows: 1. This invention enables simultaneous injection at multiple points around the injection area using an injection needle, which solves the problem of repeated punctures and position adjustments required in traditional single-needle injection methods, reduces operation time, improves the uniformity of drug distribution in tissues, and thus improves the accuracy and repeatability of drug efficacy evaluation. 2. The present invention sets the overall height benchmark through the first adjustment component and adjusts the needle tip puncture depth through the second adjustment component, which effectively avoids the problem of difficulty in accurately controlling the manual needle insertion depth, prevents the drug from overflowing due to shallow insertion or penetrating and damaging deep organs due to excessive insertion, and ensures that the injection depth is consistent each time, thereby enhancing the reliability and consistency of experimental results. 3. The present invention sets a one-way flow structure in the flow channel between the injection chamber and the dispensing chamber to ensure that the drug can only flow from the injection chamber into the dispensing chamber in one direction, and prevents the drug from flowing back or air from flowing back, thus solving the dosage error problem caused by the drug flowing back into the supply pipeline, thereby further ensuring the accuracy of quantitative injection and the reliability of experimental data. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of a subcutaneous multi-point injection device for mice according to a specific embodiment of the present invention; Figure 2 This is a cross-sectional view of a subcutaneous multi-point injection device for mice according to a specific embodiment of the present invention; Figure 3 For the present invention Figure 2 Enlarged view of the structure of section A; Figure 4 For the present invention Figure 2 Enlarged view of the structure of section B; Figure 5 For the present invention Figure 2 Enlarged view of the structure of section C; Figure 6 This is a perspective view of a multi-point subcutaneous injection device for mice according to a specific embodiment of the present invention; Figure 7 This is a bottom view showing the relative position of the injection needle and the annular base in a specific embodiment of the present invention; Figure 8 This is a schematic diagram of the structure of the syringe and guide cylinder in a specific embodiment of the present invention.

[0017] Explanation of reference numerals in the attached figures: 1. Positioning component; 11. Annular base; 12. Negative pressure suction cup; 13. Through hole; 14. Negative pressure chamber; 15. Air passage interface. 2. First adjusting component; 21. Support base; 22. Telescopic rod; 23. Locking element. 3. Injection assembly; 31. Bottle seat; 32. Guide tube; 33. Syringe; 331. Injection chamber; 332. Dispensing chamber; 333. Inlet; 334. Outlet; 34. First elastic element; 35. Injection needle; 36. One-way conduction structure; 361. Conical guide port; 362. Ball; 363. Second elastic element; 37. Piston; 38. Push rod. 4. Drug delivery components 5. Second adjustment component, 51. Control lever, 52. Stop. Detailed Implementation

[0018] The specific embodiments of the present invention are described below with reference to the accompanying drawings and examples: It should be noted that the structures, proportions, sizes, etc. illustrated in the accompanying drawings of this specification are only used to complement the content disclosed in the specification, so that those skilled in the art can understand and read them, and are not intended to limit the conditions under which the present invention can be implemented. Any modifications to the structure, changes in the proportions, or adjustments to the size, without affecting the effects and objectives that the present invention can produce, should still fall within the scope of the technical content disclosed in the present invention.

[0019] Furthermore, the terms such as "upper," "lower," "left," "right," "middle," and "one" used in this specification are merely for clarity of description and are not intended to limit the scope of the invention. Any changes or adjustments to their relative relationships, without substantially altering the technical content, should also be considered within the scope of the invention.

[0020] Combination Figures 1 to 8 As shown, the present invention provides a subcutaneous multi-point injection device for mice, including a positioning component 1, a first adjustment component 2, an injection component 3, and a drug delivery component 4.

[0021] Specifically, such as Figure 2 , Figure 3 As shown, the positioning component 1 includes an annular base 11 with several negative pressure suction cups 12 at its bottom. A circular through-hole 13 is located in the center of the annular base 11 to expose the subcutaneous injection area on the mouse's back. Each negative pressure suction cup 12 is connected to a negative pressure chamber 14 formed within the annular base 11 via a flow channel. The negative pressure chamber 14 is connected to an external negative pressure source via an air passage interface 15. The external negative pressure source can be a commonly used laboratory vacuum pump or negative pressure controller. In use, the annular base 11 is placed over the skin surrounding the injection area. The external negative pressure source is activated, creating negative pressure within the negative pressure chamber 14. This negative pressure, through the suction cups 12, generates an adsorption force on the skin surface, firmly attaching the annular base 11 to the mouse's body surface. This achieves a non-invasive, precise, easy-to-operate, and quickly release-ready stable fixation, effectively avoiding skin displacement caused by traditional tapes or clamps.

[0022] Before injecting the mouse, the initial height of the injection component 3 relative to the mouse's dorsal skin needs to be set to ensure that the tip of the injection needle 35 can accurately penetrate 6 mm into the mouse's subcutaneous tissue. Specifically, as follows... Figure 2 As shown, the first adjustment component 2 is installed above the positioning component 1 and includes a support base 21, a telescopic rod 22 and a locking member 23. The telescopic rod 22 consists of an inner rod and an outer rod, wherein the inner rod is sleeved in the sliding cavity of the outer rod and can move along the sliding cavity. The inner rod is connected to the support base 21, while the outer rod is fixed on the annular base 11. By adjusting the relative position of the inner rod on the outer rod, the telescopic adjustment of the support base 21 relative to the annular base 11 in the vertical direction can be realized.

[0023] In one specific embodiment, the locking member 23 is located at the connection between the inner and outer rods of the telescopic rod 22, used to lock the relative position between them after adjustment to a suitable height. Specifically, the inner and outer rods are respectively provided with corresponding screw holes arranged at intervals along the axial direction, with a spacing of 1 mm between adjacent screw holes. The locking member 23 passes through the screw hole on the outer rod and is threadedly connected to the corresponding screw hole on the inner rod, thereby fixing the inner and outer rods in a preset relative position. By selecting different combinations of screw holes corresponding to different heights, the initial height reference of the injection component 3 relative to the mouse skin can be quickly set, effectively adapting to subcutaneous injection areas of different mouse sizes or different elevation heights.

[0024] Preferably, the locking element can be a knob-type set screw with a butterfly handle, which allows for manual tightening and loosening without additional tools, thereby improving the ease of operation.

[0025] Specific steps: Place the annular base 11 over the skin around the injection area on the mouse's back. Activate the external negative pressure source, causing the negative pressure suction cup 12 to firmly adhere the annular base 11 to the mouse's surface under negative pressure. Then, loosen the locking piece 23 and move the support seat 21 vertically, causing the inner rod of the telescopic rod 22 to slide within the sliding cavity of the outer rod until the tip of the injection needle 35 approaches the skin surface on the mouse's back. Since the inner and outer rods have corresponding screw holes arranged equidistantly along the axial direction, with an adjacent screw hole spacing of 1 mm, the operator can directly determine and set the height of the support seat 21 relative to the annular base 11 based on the required injection depth by observing the screw hole positions, thereby initially determining the distance between the injection needle 35 and the skin surface.

[0026] After adjusting to the predetermined height, tighten the locking part 23 so that it simultaneously engages with the corresponding screw holes on the inner and outer rods and presses them together, thereby locking the telescopic rod 22 in the current position. This effectively prevents the support seat 21 from shifting due to the reaction force of the drug liquid during injection, thus ensuring that the injection needle 35 remains at the set initial height reference throughout the entire drug administration process.

[0027] Specifically, such as Figures 4 to 8As shown, the injection assembly 3 is mounted on the support base 21 and is cylindrical, comprising a cylindrical base 31, a guide cylinder 32, and a syringe 33 arranged sequentially from the outside to the inside. The cylindrical base 31 is mounted on the support base 21, and its bottom end extends axially toward the annular base 11. The guide cylinder 32 is axially slidably fitted inside the cylindrical base 31, and a first elastic element 34 is provided between the two. The two ends of the first elastic element 34 are respectively connected to the inner shoulder of the cylindrical base 31 and the outer edge of the guide cylinder 32 to provide a restoring elastic force for the guide cylinder 32. After the injection is completed and the pressure is released, the first elastic element 34 drives the guide cylinder 32 to return to its original position along the cylinder base 31, thereby causing the syringe 33 to move upward synchronously, so that the injection needle 35 automatically exits the injection area, thus realizing the automatic needle return operation of the injection needle 35. The syringe 33 is slidably disposed in the guide cylinder 32, and multiple sets of injection needles 35 are detachably installed at its bottom end. The injection needles 35 are evenly distributed around the circumference of the syringe 33, and their number can be configured according to the diameter of the circular hole, such as 4 needles, 6 needles or 8 needles. In this embodiment, 6 injection needles 35 are used to balance the uniformity of drug coverage and operational safety.

[0028] Preferably, the first elastic element 34 is a spring, with its two ends connected to the inner shoulder of the cylinder seat 31 and the outer edge of the guide cylinder 32, respectively. This spring has good resilience and fatigue resistance, and can drive the guide cylinder 32 and the syringe 33 to synchronously return to their original positions after injection, thereby achieving automatic withdrawal of the injection needle 35 and reducing secondary damage to the tissue.

[0029] In one specific embodiment, the syringe 33 has an injection chamber 331 and a dispensing chamber 332. The injection chamber 331 is connected to an external pipeline through the inlet 333, and the dispensing chamber 332 is connected to multiple outlets 334 respectively. Each outlet 334 corresponds to an injection needle 35, thereby ensuring that the drug solution can be evenly distributed to each injection needle 35.

[0030] To achieve unidirectional flow of liquid from the injection chamber 331 to the distribution chamber 332, a unidirectional guiding structure 36 is provided in the flow channel between the injection chamber 331 and the distribution chamber 332. This structure includes a conical guide port 361 and a ball 362. The conical guide port 361 is located in the flow channel between the injection chamber 331 and the distribution chamber 332, with its small-diameter end facing the distribution chamber 332, to ensure that the ball 362 can be pushed open and the flow channel can be opened under positive pressure. The ball 362 is located inside the conical guide port 361 to block or open the flow channel. The second elastic element 363 is limited and installed inside the conical guide port 361, with one end connected to the cavity wall of the distribution chamber 332 and the other end connected to the ball 362, pressing the ball 362 tightly inside the conical guide port 361, thereby ensuring that the ball 362 returns to its original sealing state under no-pressure conditions.

[0031] Preferably, the second elastic element 363 is a spring, which has good resilience and resistance to chemical corrosion, and can ensure that the ball 362 can be stably reset over a long period of time.

[0032] In one specific embodiment, a piston 37 is provided inside the injection chamber 331 of the syringe 33, and the piston 37 is slidably fitted onto the inner wall of the injection chamber 331. A push rod 38 is axially connected to the piston 37, and the push rod 38 can be manually pressed to push the piston 37 downward along the injection chamber 331, assisting in the complete discharge of the drug solution. It should be noted that the structure of the syringe 33, piston 37, and push rod 38 can refer to the design of conventional medical syringes, and their materials are preferably biocompatible medical-grade plastics or stainless steel, which can meet the experimental requirements of single use or high-pressure sterilization and reuse.

[0033] In one specific embodiment, the components such as the base 31, guide tube 32, syringe 33, and push rod 38 can be made of medical-grade polymer materials, and the injection needle 35 is a disposable sterile medical stainless steel needle, with specifications ranging from 26G to 33G depending on experimental requirements. The above materials comply with ISO 10993 or GB / T 16886 series medical device biological evaluation standards, meet the requirements for autoclaving or ethylene oxide sterilization, support reusability or single-use, and are suitable for local drug delivery experiments in mouse subcutaneous models.

[0034] The specific workflow is as follows: When the drug delivery component 4 is activated, it pressurizes the pipeline according to a preset program. The drug enters the injection chamber 331 through the inlet 333. Under the action of fluid pressure, the drug pushes the ball 362 to overcome the elastic force of the second elastic element 363, causing it to leave the sealed position of the conical guide port 361, thereby opening the flow channel between the injection chamber 331 and the distribution chamber 332. The drug then flows into the distribution chamber 332 and is evenly distributed to the corresponding injection needles 35 through multiple outlets 334, thereby achieving multi-point synchronous and equal-volume injection.

[0035] After the metered injection is completed, the metered injection pump 42 stops supplying liquid, and the pressure in the system drops rapidly. At this time, the ball 362 automatically moves back under the restoring force of the second elastic element 363, re-fitting the conical surface of the conical guide port 361 to form a seal, effectively preventing the liquid from flowing back from the dispensing chamber 332 to the injection chamber 331, while also preventing external air from flowing back into the flow channel. When it is necessary to completely empty the residual liquid, the push rod 38 can be manually operated to drive the piston 37 to move axially along the injection chamber 331, further pushing out the residual liquid in the chamber, thereby improving the accuracy of drug administration.

[0036] Specifically, the second adjustment component 5 is used to adjust the needle tip insertion depth and includes a control rod 51 and a stop 52. The end of the control rod 51 is rotatably connected to the syringe 33, and its body has a threaded section. The guide cylinder 32 has a threaded hole that mates with the threaded section, and the two form a threaded connection. In actual operation, rotating the control rod 51, since one end is restricted to rotating only on the syringe 33, drives the syringe 33 to move axially along the guide cylinder 32 through the threaded connection between the guide cylinder 32 and the control rod 51. This drives the injection needle 35 at the bottom of the syringe 33 to move downward synchronously, thereby adjusting the insertion depth of the injection needle 35 relative to the mouse skin.

[0037] In one specific embodiment, the outer wall of the syringe 33 is provided with depth scale lines along the axial direction. The scale lines are calibrated in millimeters, and the zero point is flush with the bottom end of the guide cylinder 32. During operation, by observing the position of the syringe 33 relative to the reference mark on the guide cylinder 32 and combining the scale line readings, the insertion depth of the injection needle 35 can be set intuitively and accurately, facilitating rapid calibration by the experimenter and thus improving operational efficiency and injection consistency.

[0038] In one specific embodiment, the second adjustment component 5 further includes a stop 52, which is threadedly connected to the threaded section of the control rod 51 and configured to abut against the guide cylinder 32 in a tightened state to restrict the axial movement of the syringe 33 relative to the guide cylinder 32.

[0039] Preferably, the stop 52 can be a locking nut. After the injection needle 35 is adjusted to the correct position, the locking nut is tightened so that its end face abuts against the syringe 33, thereby limiting the axial displacement of the syringe 33 relative to the guide cylinder 32.

[0040] The drug delivery assembly 4 includes a medicine container and a metering pump. The medicine container contains the drug solution to be injected. The medicine container is connected to the metering pump and the inlet 333 of the injection assembly 3 in sequence through an infusion line. The inlet 333 is formed on the opening of the inlet tube, which is sealed to the infusion line through an adapter. The inlet tube includes a flexible telescopic tube that passes through a clearance groove in the side wall of the guide cylinder 32, extends into the syringe 33, and connects to the syringe 33. The inlet tube is located in the injection chamber 331 below the piston 37, thereby ensuring that the drug solution can be directly injected into the area below the piston 37 and avoiding air bubble retention.

[0041] The medicine container is equipped with a one-way valve, preferably connected to the medicine container by a sealing thread. This one-way valve is preferably a pneumatic valve, which allows outside air to enter the medicine container while preventing inside air from flowing out. It can effectively regulate the negative pressure inside the medicine container, ensuring that the medicine can be injected continuously, and also ensuring the airtightness of the medicine container to prevent the volatilization or splashing of toxic medicine from causing harm to the operator.

[0042] In another optional embodiment, the inlet tube is sealed and connected to the syringe 33 via a one-way flow structure 36. This one-way flow structure 36 is consistent with the one-way flow structure 36 between the dispensing chamber 332 and the injection chamber 331. When the piston 37 moves towards the injection needle 35, the one-way flow structure 36 is not open. When the piston 37 moves towards the tail end of the syringe 33, the one-way flow structure 36 opens, and the metering pump pushes the drug solution into the injection chamber 331. This one-way valve is configured to allow the drug solution to flow unidirectionally from the metering pump into the injection chamber 331 and prevent the drug solution from flowing back into the infusion line or air from being drawn into the drug container, thereby further improving the sealing and dosage stability of the drug delivery component 4.

[0043] The specific operating steps of this invention are as follows: S1. After anesthetizing the experimental mice by intraperitoneal injection, they were fixed prone on the operating table, the hair on their backs was shaved and disinfected, and a round hole was cut to fully expose the subcutaneous tissue. S2. Rotate the control lever 51 of the second adjustment component 5 to adjust the axial position of the syringe 33 and guide cylinder 32 relative to the cylinder seat 31, so that the final insertion depth of the injection needle 35 meets the experimental requirements. After adjustment, tighten the stop 52 to prevent the syringe 33 from retracting during the injection process. S3. Place the positioning component 1 on the skin around the round hole to ensure that the through hole 13 completely covers the round hole. Turn on the external negative pressure source so that the negative pressure suction cup 12 can be adsorbed onto the skin, thereby fixing the positioning component 1 firmly to the mouse body surface. S4. Loosen the locking member 23 of the first adjustment component 2, adjust the length of the telescopic rod 22 so that the needle tip of the injection needle 35 is about 6 mm away from the subcutaneous skin, and then tighten the locking member 23 to lock the height; S5. Start the metering pump, prefill the tubing and expel air bubbles, the piston 37 moves to the end of the syringe 31, the one-way conduction structure 36 on the inlet tube opens, and the metering pump pushes the drug solution to the injection chamber 331 according to the preset program, but does not inject it into the tumor at the moment. S6. Manually press down on the syringe 33 and guide cylinder 32 to overcome the elastic force of the first elastic element 34, so that the guide cylinder 32 and syringe 33 move down synchronously. The injection needle 35 penetrates the tissue to the preset depth. Then, push the push rod 38 to drive the piston 37 to move down. The liquid medicine passes through the one-way guiding structure 36, the liquid distribution chamber 332 and the liquid outlet 334 respectively, and is finally injected into each injection point synchronously from the injection needle 35, thereby completing multi-point quantitative drug administration. S7. After the injection is completed, release the applied external force. Under the reset elastic force of the first elastic element 34, the guide cylinder 32 and the syringe 33 move upward automatically, the injection needle 35 exits the injection area, the negative pressure source is turned off, the negative pressure suction cup 12 is released, the entire device is removed, and the operation is completed.

[0044] Many other changes and modifications can be made without departing from the concept and scope of this invention. It should be understood that this invention is not limited to the specific embodiments, and the scope of this invention is defined by the appended claims.

Claims

1. A device for subcutaneous multi-site injection in mice, characterized in that, The injection assembly includes an injection chamber, which has an inlet and multiple outlets, all of which are evenly distributed. Multiple sets of injection needles are connected to corresponding liquid outlets. The injection assembly adjusts the injection depth of the injection needles so that the needle tip reaches the target injection area. Positioning components are used to adhere and fix the device to the mouse skin around the injection area; The first adjustment component is mounted on the positioning component, and the injection component is mounted on the adjustment component to adjust the distance between the tip of the injection needle and the mouse skin. The drug delivery assembly includes a drug container and a metering pump. The drug container is connected to the inlet of the injection assembly via a pipeline. The metering pump is located on the pipeline and is used to deliver the drug solution to the injection chamber according to a preset dose, so as to realize multi-needle metered injection.

2. The subcutaneous multi-point injection device for mice according to claim 1, characterized in that, The injection assembly has a cylindrical structure, and multiple sets of injection needles are distributed around the circumference of the cylindrical injection assembly.

3. The subcutaneous multi-point injection device for mice according to claim 1, characterized in that, The first adjustment component includes a support base for supporting the injection component; A telescopic rod is connected between the support base and the positioning component to adjust the height of the support base relative to the positioning component; A locking element, disposed on the telescopic rod, is configured to lock the telescopic rod in its telescopic state after adjustment, thereby limiting the initial height of the injection assembly and the injection needle connected thereto relative to the bottom surface of the positioning assembly.

4. The subcutaneous multi-point injection device for mice according to claim 1, characterized in that, The injection assembly includes a barrel seat disposed on the first adjustment assembly; The guide cylinder is movably fitted onto the cylinder base; A syringe is movably mounted on a guide cylinder. The syringe has an injection chamber and a piston is provided thereon to push the liquid medicine in the injection chamber through the outlet to the injection needle. The injection needle is installed at the bottom end of the syringe. The second adjustment component connects the syringe and the guide cylinder to adjust the axial position of the syringe relative to the guide cylinder, thereby adjusting the distance between the needle tip of the injection needle and the bottom surface of the syringe base.

5. The subcutaneous multi-point injection device for mice according to claim 4, characterized in that, The guide cylinder is provided with an elastic element, the two ends of which abut against the guide cylinder and the cylinder seat respectively. When the guide cylinder loses the action of external force, the elastic element is configured to drive the guide cylinder to reset.

6. The subcutaneous multi-point injection device for mice according to claim 4, characterized in that, The syringe has a dispensing chamber, one end of which is connected to the injection chamber and the other end is connected to multiple outlets. A one-way flow structure is provided in the flow channel between the dispensing chamber and the injection chamber to allow the drug solution to flow unidirectionally from the injection chamber into the dispensing chamber.

7. The subcutaneous multi-site injection device for mice according to claim 6, characterized in that, The unidirectional guiding structure includes a conical guide orifice and a ball. The conical guide orifice is formed at the communication channel between the liquid distribution chamber and the liquid injection chamber, with its small diameter end facing the liquid injection chamber. The ball is limited within the conical guide orifice by a spring.

8. The subcutaneous multi-point injection device for mice according to claim 4, characterized in that, The second adjustment component includes a control rod, the end of which is rotatably connected to a syringe or a guide cylinder. The control rod has a threaded section, and one of the syringe or the guide cylinder is threadedly connected to the threaded section so as to adjust the axial position of the syringe relative to the guide cylinder by rotating the control rod.

9. A subcutaneous multi-site injection device for mice according to claim 8, characterized in that, The second adjustment assembly further includes a stop member threadedly connected to the threaded section of the control rod and configured to abut against the syringe or the guide tube in a tightened state to restrict axial movement of the syringe relative to the guide tube.

10. A subcutaneous multi-point injection device for mice according to claim 1, characterized in that, The positioning component includes an annular base with a plurality of negative pressure suction cups on the bottom surface of the annular base. The negative pressure suction cups are connected to a negative pressure cavity formed in the annular base. The negative pressure cavity is connected to an external negative pressure source through an air passage interface to form an adsorption force at the negative pressure suction cups, thereby detachably fixing the annular base to the mouse skin.

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