An anti-inflammatory soothing composition containing oxidized trans-resveratrol and apigenin and a preparation method and application thereof

Abstract

The present application provides an anti-inflammatory soothing composition containing oxyresveratrol and rengyoside, and belongs to the technical field of cosmetics. The anti-inflammatory soothing composition comprises the following components: oxyresveratrol 0.01-0.5 parts; rengyoside 0.1-10 parts; Alpinia zerumbet leaf extract 0.05-2 parts; Phellodendron amurense bark extract 0.02-0.5 parts; wherein the mass ratio of oxyresveratrol to rengyoside is 1:(10-20). The anti-inflammatory soothing composition can play a good anti-inflammatory, soothing and repairing role through the synergistic cooperation of the four components, is beneficial to improving skin tolerance, is suitable for sensitive skin, and has good market application value.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and in particular to an anti-inflammatory and soothing composition containing oxidized resveratrol and senna-2-glucosinolate, its preparation method, and its application. Background Technology

[0002] Inflammation is the body's defensive response to damaging factors or harmful stimuli, but excessive or prolonged inflammation can lead to tissue damage. The inflammatory cascade involves multiple signaling pathways, including the NF-κB pathway, the MAPK pathway, and the JAK-STAT pathway. These pathways are interconnected, and single interventions often fail to achieve ideal results. Furthermore, ultraviolet (UV) radiation is a significant external trigger for skin inflammation, inducing the production of reactive oxygen species (ROS), activating multiple inflammatory signaling pathways within skin cells, and causing photodamage and photoaging.

[0003] While existing anti-inflammatory ingredients (such as dipotassium glycyrrhizate and bisabolol) are effective, they target only a single site and often have limited efficacy against complex inflammatory networks. Therefore, developing a multi-target, synergistic, safe, and gentle natural anti-inflammatory composition has significant advantages. Summary of the Invention

[0004] The purpose of this invention is to overcome the shortcomings of existing technologies and provide an anti-inflammatory and soothing composition containing oxidized resveratrol and senna-containing glycosides, as well as its preparation method and application. The anti-inflammatory and soothing composition of this invention can exert good anti-inflammatory, soothing, and repairing effects, which is beneficial for improving skin tolerance and suitable for use on sensitive skin.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows: In a first aspect, the present invention provides an anti-inflammatory and soothing composition containing oxidized resveratrol and senna-based glycosides, comprising the following components in parts by weight: Oxidized resveratrol 0.01-0.5 parts; Sacred herbaceous glycosides 0.1-10 parts; Alpinia zerumbet leaf extract 0.05-2 parts; 0.02-0.5 parts of Phellodendron bark extract; The mass ratio of oxidized resveratrol to senna glycoside is 1:(10-20).

[0006] The present invention provides an anti-inflammatory and soothing composition comprising oxidized resveratrol, sennaoside, ginger leaf extract, and phellodendron bark extract.

[0007] Oxidized resveratrol can exert its anti-inflammatory effect by inhibiting the NF-κB signaling pathway, thereby significantly reducing the expression of various inflammatory factors such as TNF-α, IL-1β, and IL-6.

[0008] Saussureanin can reduce UV-induced ROS and prevent inflammation; in addition, saussureanin can also block the signal transduction from extracellular stimulation to intracellular response by inhibiting the phosphorylation of MAPK kinase.

[0009] Flavonoids in the extract of Alpinia zerumbet leaf can upregulate the expression of miR-146a-5p, target and inhibit TRAF6, thereby blocking the overactivation of the downstream NF-κB inflammatory pathway, and ultimately reducing the production of multiple key inflammatory factors such as TNF-α, IL-1β, and IL-6.

[0010] The alkaloids in the bark extract of Phellodendron amurense can effectively inhibit the JAK-STAT signaling pathway, thereby reducing the production of pro-inflammatory cytokines.

[0011] The rational combination of the four components allows sennaoside to scavenge ROS and inhibit MAPK phosphorylation upstream of the inflammatory signaling pathway, while oxidized resveratrol and Alpinia galanga leaf extract inhibit the NF-κB pathway downstream, forming a comprehensive coverage from the source to the end. Furthermore, this invention works through the two core inflammatory pathways of NF-κB and JAK-STAT, and complements the epigenetic (miRNA-regulated) mechanism of inflammation inhibition, thus exerting a more comprehensive anti-inflammatory effect.

[0012] Preferably, the preparation method of the Alpinia zerumbet leaf extract includes the following steps: Take leaves of Alpinia galanga, wash them, dry them in an oven at 50-60℃ until constant weight, pulverize them, and pass them through a 50-60 mesh sieve to obtain Alpinia galanga leaf powder. Take a certain amount of the above powder, add 20-30 times the amount of 70% ethanol solution, heat to 50-60℃ and reflux for 2 hours, repeat 3 times, combine the extracts, filter, collect the filtrate, use a rotary evaporator at 50-60℃ and vacuum conditions to concentrate the filtrate to 1 / 3 of the original volume, collect the concentrate, freeze dry, and obtain the above-mentioned Alpinia galanga leaf extract.

[0013] Using the Alpinia zerumbet leaf extract prepared by the above method in the composition can achieve better anti-inflammatory and soothing effects.

[0014] Preferably, the anti-inflammatory and soothing composition comprises the following components: Oxidized resveratrol 0.05-0.3 parts; Sacred herbaceous glycosides 0.5-6 parts; Alpinia zerumbet leaf extract 0.1-1 part; 0.05-0.2 parts of Phellodendron bark extract.

[0015] More preferably, the anti-inflammatory and soothing composition comprises the following components: 0.1 parts of oxidized resveratrol; 1.5 parts of senna-based glycosides; 0.3 parts of Alpinia zerumbet leaf extract; 0.1 part of Phellodendron bark extract.

[0016] In a second aspect, the present invention also provides a method for preparing the anti-inflammatory and soothing composition described in the first aspect, comprising the following steps: Take the prescribed amount of oxidized resveratrol, add 10 parts of butylene glycol, and stir until completely dissolved; then take the prescribed amounts of senna glycoside, ginger leaf extract, and phellodendron bark extract, mix them, add water to 20 parts, and stir until completely dissolved; mix the above alcohol solution with the aqueous solution to obtain the composition.

[0017] Thirdly, the present invention also provides the use of the anti-inflammatory and soothing composition described in the first aspect in the preparation of cosmetics.

[0018] Specifically, the cosmetics are toners, lotions, creams, masks, or gels.

[0019] Preferably, the amount of the anti-inflammatory and soothing composition added is 1%-10% of the total weight of the cosmetic.

[0020] Fourthly, the present invention also provides an anti-inflammatory and soothing lotion comprising the following ingredients by weight percentage: The anti-inflammatory and soothing composition described in the first aspect is 1%-10%; Moisturizer 1%~20%; Chelating agent 0.01%~0.1%; Thickener 0.01%~1%; Emollients 1%~10%; Emulsifier 0.5%~5%; Antioxidant 0.1%~5%; pH adjuster 0.01%~0.5%.

[0021] Specifically, the raw material is selected from at least one of (a)-(g): (a) The moisturizer includes at least one of glycerin, propylene glycol, butylene glycol, and 1,2-hexanediol; (b) The chelating agent is disodium EDTA; (c) The thickener includes at least one of xanthan gum, acrylic (ester) copolymer, ammonium acryloyl dimethyl taurate / VP copolymer, hydroxyethyl cellulose, carboxymethyl cellulose, and sodium alginate; (d) The emollient includes at least one of caprylic / capric triglyceride, polydimethylsiloxane, and cetearyl alcohol; (e) The emulsifier comprises at least one of C14-22 alkyl alcohol / C12-20 alkyl glucoside, cetearyl alcohol, and polyglycerol-6 distearate; (f) The antioxidant is p-hydroxyacetophenone; (g) The pH adjuster includes at least one of arginine and citric acid.

[0022] Fifthly, the present invention also provides a method for preparing the anti-inflammatory and soothing emulsion described in the first aspect, comprising the following steps: (1) Mix the A phase component with water and stir, heat to 75-85℃, then homogenize at 6000-9000r / min for 3min, keep warm after homogenization, and obtain the pre-prepared A phase; the A phase component includes a humectant, a chelating agent and a thickener; (2) Mix the B phase components, heat to 75-85℃ until completely dissolved, keep warm for later use, and obtain the pre-made B phase; the B phase components include emollients and emulsifiers; (3) Mix the C phase components and heat to 55-65℃ to completely dissolve them to obtain a pre-prepared C phase; the C phase components include humectants and antioxidants; (4) Heat the pre-prepared phase A to 80-85°C, add the pre-prepared phase B, homogenize at 6000-9000 r / min for 3-6 min, then cool down to 50°C, add the pre-prepared phase C at 300 r / min and stir to mix, then cool down to below 45°C and add the composition described in the first aspect, continue stirring for 5 min, finally add pH adjuster to adjust pH, then stop stirring, discharge the material, and obtain the emulsion.

[0023] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention provides an anti-inflammatory and soothing composition containing oxidized resveratrol and senna-based glycosides, and an anti-inflammatory and soothing lotion prepared using this composition. Experimental data show that the anti-inflammatory and soothing composition provided by this invention has a TNF-α inhibition rate of over 45%, and the anti-inflammatory and soothing lotion has a TEWL value improvement rate of over 20%, an a* value improvement rate of over 10%, and a lactic acid stinging improvement rate of over 55%. This indicates that the anti-inflammatory and soothing composition of this invention has good anti-inflammatory, soothing, and repairing effects through the synergistic effect of its four components, which is beneficial for improving skin tolerance and suitable for use on sensitive skin. Detailed Implementation

[0024] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0025] Unless otherwise specified, all other materials and reagents used in the examples are commercially available.

[0026] Some of the raw materials used in this invention are from the following sources: Oxidized resveratrol: purchased from Naturalis Srl, product name OXYRESVERATROL; Resveratrol: Purchased from Guangzhou Kesa Road Trading Co., Ltd., product name is resveratrol; Sacred herb glycoside: Purchased from Xi'an Shilin Biotechnology Co., Ltd., product name is Sacred herb glycoside; Phellodendron bark extract: purchased from Guangzhou Heji Biotechnology Co., Ltd., product name: Phellodendron bark extract; The extract from the leaves of Alpinia zerumbet was prepared in-house, and the extraction method is as follows: Alpinia zerumbet leaf extract 1: Take commercially available Alpinia zerumbet leaves, wash them, dry them in an oven at 55℃ to constant weight, pulverize them, and pass them through a 50-mesh sieve to obtain Alpinia zerumbet leaf powder; take a certain amount of Alpinia zerumbet leaf powder, add 25 times the amount of 70% ethanol solution, heat to 55℃ and reflux for 2 hours, repeat 3 times, combine the extracts, filter, collect the filtrate, use a rotary evaporator at 55℃ and vacuum conditions to concentrate the filtrate to 1 / 3 of the original volume, collect the concentrate, freeze dry it to obtain Alpinia zerumbet leaf extract 1; Alpinia zerumbet leaf extract 2: Take commercially available Alpinia zerumbet leaves, wash them, air dry them to constant weight, then grind them into fine powder using a pulverizer, pass them through a No. 5 sieve, add 3 times the weight of the fine powder in 70% ethanol solution, extract 4 times at 25℃, combine the filtrates, recover them in a rotary evaporator until there is no alcohol odor, add an appropriate amount of hot water to suspend them, and then filter them to obtain Alpinia zerumbet leaf extract 2.

[0027] The components and their contents (parts by mass) of the anti-inflammatory and soothing compositions of different embodiments and comparative examples of the present invention are shown in the table below: Table 1 Example 1 0.1 / 1.5 0.3 / 0.1 Example 2 0.05 / 0.5 0.1 / 0.05 Example 3 0.3 / 6 1 / 0.2 Example 4 0.01 / 0.1 0.05 / 0.02 Example 5 0.5 / 10 2 / 0.5 Example 6 0.1 / 1.5 / 0.3 0.1 Comparative Example 1 0.02 / 0.1 0.3 / 0.1 Comparative Example 2 0.4 / 10 0.3 / 0.1 Comparative Example 3 / 0.1 1.5 0.3 / 0.1 Comparative Example 4 / / 1.5 0.3 / 0.1 Comparative Example 5 0.1 / / 0.3 / 0.1 Comparative Example 6 0.1 / 1.5 / / 0.1 Comparative Example 7 0.1 / 1.5 0.3 / / Comparative Example 8 0.005 / 0.05 2 / 0.5 Comparative Example 9 0.75 / 15 0.05 / 0.02 Comparative Example 10 0.5 / 10 0.02 / 0.01 Comparative Example 11 0.01 / 0.1 2.5 / 0.8 The preparation methods of the anti-inflammatory and soothing compositions provided in Examples 1-6, Comparative Examples 1-2, and Comparative Examples 8-9 of this invention include the following steps: Take the prescribed amount of oxidized resveratrol, add 10 parts of butylene glycol, and stir until completely dissolved; then take the prescribed amounts of senna glycoside, ginger leaf extract, and phellodendron bark extract, mix them, add water to 20 parts, and stir until completely dissolved; mix the above alcohol solution with the aqueous solution to obtain the anti-inflammatory and soothing composition.

[0028] The preparation method of the anti-inflammatory and soothing composition provided in Comparative Example 3 of the present invention includes the following steps: Take the prescribed amount of resveratrol, add 10 parts of butylene glycol, and stir until completely dissolved; then take the prescribed amounts of senna glycoside, ginger leaf extract, and phellodendron bark extract, mix them, add water to 20 parts, and stir until completely dissolved; mix the above alcohol solution with the aqueous solution to obtain the anti-inflammatory and soothing composition.

[0029] The preparation method of the anti-inflammatory and soothing composition provided in Comparative Example 4 of the present invention includes the following steps: Take the prescribed amounts of senna-based glycosides, ginger leaf extract, and phellodendron bark extract, mix them, add water to 20 parts, and stir until completely dissolved; mix 10 parts of butylene glycol with the above aqueous solution to obtain the anti-inflammatory and soothing composition.

[0030] The preparation method of the anti-inflammatory and soothing composition provided in Comparative Example 5 of the present invention includes the following steps: Take the prescribed amount of oxidized resveratrol, add 10 parts of butylene glycol, and stir until completely dissolved; then take the prescribed amounts of ginger leaf extract and phellodendron bark extract, mix them, add water to 20 parts, and stir until completely dissolved; mix the above alcohol solution with the aqueous solution to obtain the anti-inflammatory and soothing composition.

[0031] The preparation method of the anti-inflammatory and soothing composition provided in Comparative Example 6 of the present invention includes the following steps: Take the prescribed amount of oxidized resveratrol, add 10 parts of butylene glycol, and stir until completely dissolved; then take the prescribed amount of senna glycoside and phellodendron bark extract, mix them, add water to 20 parts, and stir until completely dissolved; mix the above alcohol solution with the aqueous solution to obtain the anti-inflammatory and soothing composition.

[0032] The preparation method of the anti-inflammatory and soothing composition provided in Comparative Example 7 of the present invention includes the following steps: Take the prescribed amount of oxidized resveratrol, add 10 parts of butylene glycol, and stir until completely dissolved; then take the prescribed amount of senna and ginger leaf extract, mix them, add water to 20 parts, and stir until completely dissolved; mix the above alcohol solution with the aqueous solution to obtain the anti-inflammatory and soothing composition.

[0033] The components and their contents (weight percentage %) of the anti-inflammatory and soothing lotions provided in various application examples and comparative application examples of the present invention are shown in the table below: Table 2

[0034] In these examples, the anti-inflammatory and soothing compositions in Application Examples 1-6 and Comparative Application Examples 1-11 are the anti-inflammatory and soothing compositions of Examples 1-6 and Comparative Examples 1-11, respectively. No anti-inflammatory and soothing composition was added in the blank application examples.

[0035] The methods for preparing soothing lotions provided in the various application examples and comparative application examples of this invention include the following steps: 1) Mix each component of phase A with water and stir. Heat to 80°C and homogenize at 8000 r / min for 3 min. After homogenization, keep warm for later use to obtain the pre-prepared phase A. 2) Mix the components of phase B, heat to 80°C until completely dissolved, keep warm for later use, and obtain the pre-prepared phase B; 3) Mix the components of phase C and heat to 60°C until completely dissolved to obtain the pre-prepared phase C; 4) Heat the pre-prepared phase A to 85°C, add the pre-prepared phase B, homogenize at 7000 r / min for 5 min, then cool to 50°C, add the pre-prepared phase C at 300 r / min and stir to mix, then cool to below 45°C and add the corresponding anti-inflammatory and soothing composition, continue stirring for 5 min, finally add arginine to adjust the pH, then stop stirring, discharge the material to obtain the emulsion.

[0036] The anti-inflammatory and soothing compositions of the various embodiments and comparative examples of the present invention, as well as the efficacy testing methods for various application examples, comparative application examples, and blank application examples, include: 1. Test method for the anti-inflammatory effect of anti-inflammatory and soothing compositions: S1. Plating: Logarithmically growing RAW264.7 cells were seeded into 24-well plates at a cell density of 2 × 10⁻⁶ cells / well. 5 Cells / mL, 0.5mL per well. After the plate is prepared, mark the cell plate cover (divided into blank control group, model group, positive control group, and experimental group). Incubate in a carbon dioxide incubator for 24 h. Each group has three replicates. S2. Sample processing: After cell incubation for 24 hours, aspirate the supernatant and add 0.5 mL of the following samples respectively: ① Blank control group: DMEM culture medium; ② Model group: DMEM medium containing 1 μg / mL LPS; ③ Positive control group: DMEM medium containing 0.001 wt% dexamethasone and 1 μg / mL LPS; ④ Experimental group: DMEM medium containing 0.5wt% anti-inflammatory and soothing composition and 1μg / mL LPS; S3. Detection of inflammatory factors in cell supernatants of each group: After treating cells with the anti-inflammatory and soothing composition for 24 hours, the supernatant was pipetted into centrifuge tubes, centrifuged at 1000 rpm for 5 minutes, the precipitate was discarded, and the supernatant was collected. The changes in inflammatory factors in the supernatant were detected using the Xinbosheng TNF-α inflammatory factor detection kit. The results are expressed as TNF-α inhibition rate. The formula for calculating the TNF-α inhibition rate is as follows: TNF-α inhibition rate (%) = [(TNF-α level in model group - TNF-α level in experimental group) / TNF-α level in model group] × 100%.

[0037] 2. Test method for the skin soothing and repairing effect of anti-inflammatory and soothing lotion: Anti-inflammatory and soothing lotions from various application examples, control application examples, and blank application examples were used as test samples to test their skin soothing and repair effects. The method is as follows: (1) Several volunteers aged 18-45 were selected and randomly divided into 18 groups of 6 people each. During the test, each group randomly used the lotion of each application example, control example, and blank application example of the present invention. It was used once in the morning and once in the evening. The method of use was to take 1 g of sample and apply it evenly to the whole face after cleansing and moisturizing in the morning and evening, and gently massage until fully absorbed. Follow-up visits were conducted on days 0 and 14 to test various indicators.

[0038] (2) On the days of follow-up visits on days 0 and 14, the subjects did not apply any skin care products. After cleansing, they sat quietly for 30 minutes in a constant temperature and humidity room with a temperature of 21±1℃ and a humidity of 50±10%. The initial TEWL value of the subjects after cleansing was tested using the Tewameter®™ Hex (Courage+Khazaka) transdermal moisture loss probe. The Visia-7 was used to take facial photos, and the exported red area images were analyzed to obtain the a* value. (3) Analyze the improvement of each indicator, and take the average value of the data. The improvement is calculated as follows: Improvement rate (%) = [( X 使用后 - X 使用前 ) / X 使用前 ×100%; In the formula: X 使用前 Data on various indicators tested before subjects used the product; X 使用后 Data on various indicators tested after subjects used the product.

[0039] 3. Test method for the suitability of anti-inflammatory and soothing lotion for sensitive skin: Using anti-inflammatory and soothing lotions from various application examples, comparative application examples, and blank application examples as test samples, the suitability of the product for sensitive skin groups was determined by assessing the improvement rate of lactic acid stinging before and after use.

[0040] Volunteer selection: 90 people with sensitive skin were selected as volunteers and divided into 18 groups of 5 people each.

[0041] Test procedure: The test was conducted on the nasolabial folds of volunteers. 50 μL of 10wt% lactic acid solution was dropped onto a single layer of filter paper with a diameter of 0.8 cm. The subjects were asked about their stinging sensation at 0.5 min and 2.5 min. The subjects were scored using a 4-point scale (0 points for no stinging, 1 point for mild stinging, 2 points for moderate stinging, and 3 points for severe stinging).

[0042] Sample usage: Volunteers in the sample group applied the anti-inflammatory and soothing lotion of each application example, control application example, and blank application example to the entire face. Volunteers applied the product twice a day, morning and evening, with 1 mL of lotion each time, as instructed, for two consecutive weeks. After two weeks of use, the above test steps were repeated.

[0043] Record the lactic acid stinging scores of volunteers before and after 2 weeks of use, and calculate the improvement rate of lactic acid stinging in volunteers.

[0044] The improvement rate of lactic acid stinging (%) was calculated as follows: [(sum of lactic acid stinging scores before use - sum of lactic acid stinging scores after 2 weeks of use) / sum of lactic acid stinging scores before use] × 100%; The sum of lactic acid stinging scores = the average lactic acid stinging score over 0.5 minutes + the average stinging score over 2.5 minutes.

[0045] The higher the improvement rate of lactic acid stinging, the better the repair effect of the sample, and the more suitable the product is for people with sensitive skin.

[0046] The anti-inflammatory and soothing effects of the anti-inflammatory and soothing compositions of various embodiments and comparative examples of the present invention, as well as the anti-inflammatory and soothing lotions of various application examples, comparative application examples, and blank application examples, are shown in the table below: Table 3 Example 1 60.7 Application Example 1 30.5 20.8 70.6 Example 2 51.5 Application Example 2 24.3 16.8 61.1 Example 3 55.8 Application Example 3 26.5 18.5 63.2 Example 4 49.3 Application Example 4 23.1 16.5 60.0 Example 5 46.9 Application Example 5 20.8 15.3 57.9 Example 6 45.2 Application Example 6 21.2 14.2 55.6 Comparative Example 1 24.0 Comparative Application Example 1 8.8 5.7 31.3 Comparative Example 2 25.2 Comparative Application Example 2 8.4 6.0 35.3 Comparative Example 3 28.7 Comparative Application Example 3 9.6 6.8 38.9 Comparative Example 4 16.8 Comparative Application Example 4 4.7 4.1 25.0 Comparative Example 5 17.9 Comparative Application Example 5 5.3 4.5 26.3 Comparative Example 6 15.1 Comparative Application Example 6 4.0 3.8 20.0 Comparative Example 7 19.7 Comparative Application Example 7 6.1 4.7 26.3 Comparative Example 8 26.9 Comparative Application Example 8 8.9 6.2 35.0 Comparative Example 9 25.1 Comparative Application Example 9 8.0 5.9 38.9 Comparative Example 10 22.3 Comparative Application Example 10 6.6 5.5 31.6 Comparative Example 11 23.5 Comparative Application Example 11 6.9 5.1 31.6 / / Blank application example 2.5 3.1 11.1 As shown in the table above, the TNF-α inhibition rate of the anti-inflammatory and soothing composition of the present invention is higher than 45%, the TEWL value improvement rate of the anti-inflammatory and soothing lotion is higher than 20%, the a* value improvement rate is higher than 10%, and the lactic acid stinging improvement rate is higher than 55%. This indicates that the anti-inflammatory and soothing composition of the present invention can play a good role in anti-inflammatory, soothing and repairing effects, which is beneficial to improving skin tolerance and suitable for use on sensitive skin.

[0047] In Comparative Example 1, the mass ratio of oxidized resveratrol to senna was greater than 1:10, and in Comparative Example 2, the mass ratio was less than 1:20. Both are outside the scope of this invention. The TNF-α inhibition rate of the compositions was significantly reduced, which further led to a significant decrease in the TEWL value improvement rate, a* value improvement rate, and lactic acid stinging relief rate of the anti-inflammatory and soothing lotion in Comparative Application Examples 1-2. The above data indicate that only when the mass ratio of oxidized resveratrol to senna is within the range defined by this invention can it effectively inhibit the NF-κB signaling pathway.

[0048] In Comparative Example 3, resveratrol was used instead of oxidized resveratrol, resulting in a decrease in TNF-α inhibition rate. Compared with the anti-inflammatory and soothing lotion in Application Example 3, the improvement rates of TEWL value, a* value, and lactic acid stinging were also significantly reduced. This indicates that under the influence of Alpinia galanga leaf extract and Phellodendron amurense bark extract, the synergistic effect of resveratrol and sennaoside in inhibiting the NF-κB signaling pathway is weaker than that of oxidized resveratrol and sennaoside.

[0049] The absence of oxidized resveratrol in Comparative Example 4 and Comparative Application Example 4, the absence of sennaoside in Comparative Example 5 and Comparative Application Example 5, the absence of Zingiber officinale leaf extract in Comparative Example 6 and Comparative Application Example 6, and the absence of Phellodendron bark extract in Comparative Example 7 and Comparative Application Example 7 all resulted in a significant decrease in the TNF-α inhibition rate of the anti-inflammatory and soothing composition, as well as a significant decrease in the TEWL value improvement rate, a* value improvement rate, and lactic acid stinging improvement rate of the anti-inflammatory and soothing emulsion. This indicates that all four components in the composition of this application are indispensable, and only when the composition contains all four components can it exert a synergistic effect.

[0050] Comparative Examples 8, 9, 10, and 11 have one or two components whose mass fractions are outside the scope of this invention. Experimental data show that not only is the TNF-α inhibition rate of the composition reduced, but it also leads to a significant decrease in the improvement rates of TEWL value, a* value, and lactic acid stinging in the anti-inflammatory and soothing lotion in Comparative Application Examples 8-11. This indicates that the mass fractions of the above four components are outside the scope defined by this invention, and the anti-inflammatory and soothing effects of the composition are significantly reduced. This demonstrates that the amount of different components plays a very important role in the efficacy of the composition.

[0051] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. An anti-inflammatory and soothing composition containing oxidized resveratrol and senna-2-glucosinolates, characterized in that, By weight, it includes the following components: Oxidized resveratrol 0.01-0.5 parts; Sacred herbaceous glycosides 0.1-10 parts; Alpinia zerumbet leaf extract 0.05-2 parts; 0.02-0.5 parts of Phellodendron bark extract; Furthermore, the mass ratio of oxidized resveratrol to senna glycoside is 1:(10-20).

2. The anti-inflammatory and soothing composition according to claim 1, characterized in that, The preparation method of the Alpinia galanga leaf extract includes the following steps: Take leaves of Alpinia galanga, wash them, dry them in an oven at 50-60℃ until constant weight, pulverize them, and pass them through a 50-60 mesh sieve to obtain Alpinia galanga leaf powder. Take a certain amount of the above powder, add 20-30 times the amount of 70% ethanol solution, heat to 50-60℃ and reflux for 2 hours, repeat 3 times, combine the extracts, filter, collect the filtrate, use a rotary evaporator at 50-60℃ and vacuum conditions to concentrate the filtrate to 1 / 3 of the original volume, collect the concentrate, freeze dry, and obtain the above-mentioned Alpinia galanga leaf extract.

3. The anti-inflammatory and soothing composition according to claim 1, characterized in that, The anti-inflammatory and soothing composition comprises the following components: Oxidized resveratrol 0.05-0.3 parts; Sacred herbaceous glycosides 0.5-6 parts; Alpinia zerumbet leaf extract 0.1-1 part; 0.05-0.2 parts of Phellodendron bark extract.

4. A method for preparing the anti-inflammatory and soothing composition according to any one of claims 1 to 3, characterized in that, Includes the following steps: Take the prescribed amount of oxidized resveratrol, add 10 parts of butanediol, stir until completely dissolved, and obtain an alcohol solution; Mix the prescribed amounts of senna-based glycosides, ginger leaf extract, and phellodendron bark extract, add water to 20 parts, and stir until completely dissolved to obtain an aqueous solution; The composition is obtained by mixing the alcohol solution with the aqueous solution.

5. The use of the anti-inflammatory and soothing composition according to any one of claims 1 to 3 in the preparation of cosmetics.

6. The application according to claim 5, characterized in that, The amount of the anti-inflammatory and soothing composition added is 1%-10% of the total weight of the cosmetic.

7. An anti-inflammatory and soothing lotion, characterized in that, The raw materials include the following weight percentages: 1%-10% of the anti-inflammatory and soothing composition according to any one of claims 1 to 3; Moisturizer 1%~20%; Chelating agent 0.01%~0.1%; Thickener 0.01%~1%; Emollients 1%~10%; Emulsifier 0.5%~5%; Antioxidant 0.1%~5%; pH adjuster 0.01%~0.5%.

8. The anti-inflammatory and soothing lotion according to claim 7, characterized in that, The raw material is selected from at least one of (a)-(g): (a) The moisturizer includes at least one of glycerin, propylene glycol, butylene glycol, and 1,2-hexanediol; (b) The chelating agent is disodium EDTA; (c) The thickener includes at least one of xanthan gum, acrylic (ester) copolymer, ammonium acryloyl dimethyl taurate / VP copolymer, hydroxyethyl cellulose, carboxymethyl cellulose, and sodium alginate; (d) The emollient includes at least one of caprylic / capric triglyceride, polydimethylsiloxane, and cetearyl alcohol; (e) The emulsifier comprises at least one of C14-22 alkyl alcohol / C12-20 alkyl glucoside, cetearyl alcohol, and polyglycerol-6 distearate; (f) The antioxidant is p-hydroxyacetophenone; (g) The pH adjuster includes at least one of arginine and citric acid.

9. The method for preparing the anti-inflammatory and soothing emulsion according to claim 7 or 8, characterized in that, Includes the following steps: (1) Mix the A phase component with water and stir, heat to 75-85℃, then homogenize at 6000-9000r / min for 3min, keep warm after homogenization, and obtain the pre-prepared A phase; the A phase component includes a humectant, a chelating agent and a thickener; (2) Mix the B phase components, heat to 75-85℃ until completely dissolved, keep warm for later use, and obtain the pre-made B phase; the B phase components include emollients and emulsifiers; (3) Mix the C phase components and heat to 55-65℃ to completely dissolve them to obtain a pre-prepared C phase; the C phase components include humectants and antioxidants; (4) Heat the pre-prepared phase A to 80-85°C, add the pre-prepared phase B, homogenize at 6000-9000 r / min for 3-6 min, then cool down to 50°C, add the pre-prepared phase C at 300 r / min and stir to mix, then cool down to below 45°C and add the anti-inflammatory and soothing composition according to any one of claims 1 to 3, continue stirring for 5 min, finally add pH adjuster to adjust pH, then stop stirring, discharge the material, and obtain the emulsion.

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