A plant-derived hair loss prevention composition containing camellia seed oil and a preparation method and application thereof

Abstract

The present application relates to the field of cosmetics, discloses a plant source hair loss prevention composition containing camellia seed oil and a preparation method and application thereof, the composition comprising: camellia seed oil 50-95 parts; 1.9-5 parts of hyperin; 0.5-10 parts of Albizia Lebbeck seed extract; 0.5-12 parts of Cannabis Sativa L. seed extract; the weight ratio of camellia seed oil to hyperin is (10-50):1; the weight ratio of Albizia Lebbeck seed extract to Cannabis Sativa L. seed extract is (0.2-5):1.The preparation method is as follows: the camellia seed oil, hyperin, Albizia Lebbeck seed extract and Cannabis Sativa L. seed extract are uniformly mixed, and then cosmetically acceptable adjuvants are added, and the mixture is stirred and emulsified at 25-50 DEG C to obtain the composition.The composition can be used in the preparation of external preparations for preventing hair loss and promoting hair growth.The present application uses camellia seed oil as a transdermal carrier, high-specification hyperin as the core, and specific proportioning of plant active groups to construct a multi-target intervention system, synergistically activate the Wnt / β-catenin pathway and inhibit 5α-reductase, realize the effect of preventing hair loss and promoting hair growth close to chemical intervention, and provide a new natural external use scheme for androgenic alopecia.

Description

Technical Field

[0001] This invention belongs to the field of cosmetics and topical skin preparations, specifically relating to a plant-derived anti-hair loss composition containing camellia seed oil, its preparation method, and its application. Background Technology

[0002] Hair loss is a very common skin appendage disorder in clinical practice, with androgenetic alopecia being the most typical type. Its pathological features are mainly characterized by hair follicle miniaturization, shortened growth phase, and premature regression phase, ultimately leading to irreversible thinning and loss of hair.

[0003] Currently, routine clinical interventions for hair loss prevention largely rely on chemically synthesized drugs such as minoxidil. However, these small-molecule chemical drugs generally have limitations such as single target, long-term dependence, and easy relapse upon discontinuation. Furthermore, their transdermal absorption often causes local contact dermatitis, itching, or more widespread systemic side effects on the scalp. Therefore, exploring natural plant-derived alternatives with high biocompatibility, safety, and comprehensive mechanisms has become a research focus in dermatology and high-end daily chemical industries.

[0004] Camellia seed oil, a high-grade woody oil unique to my country, is highly regarded in high-end topical skin preparations due to its excellent transdermal absorption and biocompatibility. Compared to conventional plant oils, camellia seed oil has a high degree of compatibility with the human sebum membrane in terms of physicochemical properties, effectively repairing damaged scalp barriers and serving as an excellent natural transdermal carrier to improve the scalp microenvironment.

[0005] However, given the extremely complex pathogenesis of hair loss, the current technologies for developing and utilizing camellia seed oil still have significant limitations in efficacy. Extensive industry practice shows that, when used alone as an intervention, camellia seed oil primarily functions at the level of superficial lipid replenishment and basic physiochemical nourishment, lacking the ability to deeply target and regulate the hair follicle stem cell cycle and related hair growth signaling pathways. Therefore, the single application of camellia seed oil cannot fundamentally reverse the miniaturization process of hair follicles, and its substantial efficacy in preventing hair loss and promoting hair growth is extremely limited, failing to meet the needs of clinical applications.

[0006] Furthermore, achieving multi-target hair loss prevention typically requires combining various high-concentration plant extracts. This easily leads to formulation instability issues such as stickiness, poor transdermal absorption, and easy demulsification. In summary, how to leverage the superior barrier repair and transdermal carrier advantages of camellia seed oil, and through scientific compounding of substances, overcome the technical bottleneck of existing plant oils lacking multi-target hair growth mechanisms, to develop novel topical compositions that combine high safety with significant hair loss prevention and hair growth promotion effects, is a technical challenge that urgently needs to be overcome by those skilled in the art. Summary of the Invention

[0007] To address the aforementioned problems, this invention provides a plant-derived anti-hair loss composition containing camellia seed oil, its preparation method, and its application.

[0008] In a first aspect, the present invention provides a plant-based anti-hair loss composition containing camellia seed oil, which adopts the following technical solution: A plant-based anti-hair loss composition containing camellia seed oil, comprising the following components by weight: 50-95 parts camellia seed oil; 1.9-5 parts St. John's wort flavonoids; 0.5-10 parts soybean seed extract; 0.5-12 parts hemp seed extract; The weight ratio of camellia seed oil to St. John's wort flavonoids is (10-50):1; the weight ratio of soybean seed extract to hemp seed extract is (0.2-5):1.

[0009] Furthermore, the plant-derived anti-hair loss composition containing camellia seed oil comprises, by weight, the following components: 70-90 parts camellia seed oil; 2-3 parts St. John's wort flavonoids; 1-6 parts edamame seed extract; 2-8 parts hemp seed extract.

[0010] Furthermore, the St. John's wort flavonoids are flavonoid extracts obtained by extraction and purification of the whole herb of St. John's wort, wherein the total flavonoid content is not less than 50%.

[0011] Furthermore, the camellia seed oil is a vegetable oil obtained by pressing or extracting the seeds of the camellia plant (Theaceae family), and its unsaturated fatty acid content is not less than 80%.

[0012] Furthermore, the edamame seed extract is an extract obtained by water extraction or alcohol extraction and purification with macroporous resin from edamame seeds, with a total flavonoid content of not less than 10% and an oligosaccharide content of not less than 15%.

[0013] Furthermore, the hemp seed extract is an extract obtained by supercritical CO2 extraction or solvent extraction of dried mature hemp seeds, and its total unsaturated fatty acid content is not less than 70%, linoleic acid content is not less than 50%, and linolenic acid content is not less than 15%.

[0014] Furthermore, the plant-derived anti-hair loss composition containing camellia seed oil also includes cosmetically acceptable excipients.

[0015] Furthermore, the cosmetic excipients acceptable to the cosmetic include one or more of solvents, emulsifiers, thickeners, humectants, preservatives, and fragrances.

[0016] Secondly, the preparation method of the plant-derived anti-hair loss composition containing camellia seed oil provided by the present invention adopts the following technical solution: A method for preparing a plant-derived anti-hair loss composition containing camellia seed oil includes the following steps: mixing camellia seed oil with St. John's wort flavonoids, edamame seed extract and hemp seed extract evenly, adding cosmetically acceptable excipients, and stirring and emulsifying at 25-50°C to obtain the final product.

[0017] Thirdly, the application of the plant-based anti-hair loss composition containing camellia seed oil provided by the present invention adopts the following technical solution: The above-mentioned plant-derived anti-hair loss composition containing camellia seed oil and / or the composition prepared by the above-mentioned method of preparing a plant-derived anti-hair loss composition containing camellia seed oil are used in the preparation of topical preparations for preventing hair loss and promoting hair growth.

[0018] Furthermore, the topical preparations include scalp serums, shampoos, conditioners, scalp care masks, and hair growth solutions.

[0019] In summary, the present invention has the following beneficial effects: 1. This invention combines camellia seed oil, St. John's wort flavonoids, soybean seed extract, and hemp seed extract in extremely strict specific weight ratios. This specific ratio produces a strong molecular-level synergistic effect in promoting dermal papillary cell proliferation, upregulating the expression of hair growth factors (VEGF, IGF-1, HGF), activating the Wnt / β-catenin core hair growth signaling pathway, and inhibiting the activity of 5α-reductase, the culprit behind hair loss. 2. The composition of the present invention works through multiple mechanisms, including activating the ERK / AKT signaling pathway, anti-inflammatory and antioxidant effects, activating the Wnt / β-catenin pathway and hair follicle stem cells, inhibiting 5α-reductase, and regulating lipid metabolism. It works in two ways, namely, blocking the target of hair loss prevention and activating the hair growth pathway, to comprehensively reverse the process of hair follicle miniaturization and effectively prolong the hair follicle growth period. 3. The core components of this invention are all derived from natural plants, avoiding the local contact dermatitis, itching, or endocrine side effects that are easily caused by the transdermal absorption of chemical drugs. Furthermore, the camellia seed oil base is highly compatible with the human sebum membrane, effectively repairing the damaged scalp barrier. The overall composition has extremely low irritation, perfectly meeting the safety requirements for long-term hair loss prevention intervention. It can also be seamlessly integrated into various external care bases such as scalp serums, shampoos, conditioning masks, and hair growth solutions, possessing high industrial application value. Detailed Implementation

[0020] The present invention will be described in detail below with reference to specific embodiments. It should be understood that these embodiments are for illustrative purposes only and are not intended to limit the scope of the invention.

[0021] Unless otherwise specified, the experimental methods used in the following examples are conventional methods. Unless otherwise specified, the materials and reagents used in the following examples are all available through conventional commercial channels.

[0022] In specific embodiments, unless otherwise stated, all raw materials used meet the following requirements: Camellia seed oil: Unsaturated fatty acid content ≥80%; sourced from Frog Prince (Fujian) Baby Care Products Co., Ltd. St. John's wort flavonoids: Total flavonoid content ≥50%; sourced from Shanghai Jiayu Biotechnology Co., Ltd. Grey soybean seed extract: total flavonoid content ≥10%, oligosaccharide content ≥15%; from Shanghai Jiayu Biotechnology Co., Ltd. Hemp seed extract: total unsaturated fatty acid content ≥70%, linoleic acid ≥50%, linolenic acid ≥15%; sourced from Shanghai Jiayu Biotechnology Co., Ltd. Excipients: All use equal amounts of basic excipients: 60 parts deionized water, 5 parts glycerin, and 0.8 parts xanthan gum; Phenoxyethanol / ethylhexylglycerin is from EUXYL® PE 9010.

[0023] Examples 1-6 A plant-based anti-hair loss composition containing camellia seed oil, wherein the amounts of each component are as follows, by weight.

[0024]

[0025] And it is obtained by the following method: (1) Slowly add xanthan gum to deionized water and stir at room temperature until it swells. Then add glycerol and mix well to obtain a thickened aqueous matrix. (2) Camellia seed oil, St. John's wort flavonoids, edamame seed extract and hemp seed extract were mixed evenly to obtain a plant-derived mixed oil phase. (3) Add the plant-derived mixed oil phase obtained in step (2) to the thickened aqueous matrix in step (1), and stir and homogenize at a speed of 800 r / min for 30 minutes under a constant temperature of 35°C, so that the oil phase is uniformly suspended in the aqueous matrix, thereby obtaining the plant-derived anti-hair loss composition containing camellia seed oil.

[0026] Examples 7-10 A plant-derived anti-hair loss composition containing camellia seed oil is prepared in a manner basically the same as in Example 1, with the only difference being the amount of each component used, as detailed in the table below.

[0027]

[0028] Example 11 A plant-derived anti-hair loss composition containing camellia seed oil is prepared in a manner basically the same as in Example 1, except that St. John's wort flavonoids are replaced with an equal amount of commercially available 20% pure St. John's wort crude extract.

[0029] Example 12 A plant-derived anti-hair loss composition containing camellia seed oil is prepared in a manner basically the same as in Example 1, except that commercially available camellia seed oil is used, and its unsaturated fatty acid content is 68%.

[0030] Example 13 A plant-derived anti-hair loss composition containing camellia seed oil is prepared in a manner basically the same as in Example 1, except that commercially available edamame seed extract is used, which has a total saponin content of 10% and a polysaccharide content of 13%.

[0031] Example 14 A plant-derived anti-hair loss composition containing camellia seed oil is prepared in a manner similar to that of Example 1, with the only difference being that commercially available hemp seed extract is used, which has a total unsaturated fatty acid content of 65%, a linoleic acid content of 40%, and a linolenic acid content of 10%.

[0032] Comparative Examples 1-5 A composition is prepared in a manner basically the same as in Example 1, with the only difference being the different uses of each component, as detailed in the table below.

[0033]

[0034] Application Example 1 A hair loss prevention scalp serum, with a total quantity of 100 parts, has the following dosage of each component.

[0035]

[0036] And it is prepared by the following process: 1. Mix propylene glycol and ethanol evenly, then add tocopheryl acetate and stir until completely dissolved to form an alcohol-soluble phase; 2. Add phenoxyethanol / ethylhexylglycerin to a predetermined amount of deionized water, stir and mix thoroughly to form an aqueous phase; 3. Add the alcohol-soluble phase obtained in step 1 to the aqueous phase obtained in step 2, stir evenly, and obtain the essence matrix; 4. Add the plant-derived anti-hair loss composition containing camellia seed oil obtained in Example 1 to the essence matrix, and stir until homogenized at 35°C to obtain the anti-hair loss scalp essence.

[0037] Performance testing 1. Human dermal papillary cell proliferation experiment Experimental objective: Human dermal papillary cells (HDPCs) are located at the base of hair follicles and are core functional cells that regulate the hair follicle growth cycle, especially inducing and maintaining the hair growth phase and promoting hair regeneration. This experiment aims to verify the effect of the composition of the present invention on targeted activation of hair follicle cells by evaluating the promoting effect of each sample on the proliferation activity of HDPCs cultured in vitro.

[0038] Experimental methods: Human dermal papillary cells (HDPC) were cultured in vitro for testing. Cells were seeded in 96-well plates, and the following samples were added (final concentration based on total composition; all samples were diluted to 0.1%). The test groups included: each example and comparative example, and minoxidil positive control group (10 μM); After 48 hours of culture, cell proliferation activity was detected using the CCK-8 assay, and the cell proliferation rate was calculated.

[0039] The test results are as follows.

[0040]

[0041] Comparing the data of Example 1 with those of Comparative Examples 2-5, it can be seen that when camellia seed oil, St. John's wort flavonoids, soybean seed extract or hemp seed extract are used alone, although they have a certain cell proliferation promoting effect compared with the blank group (Comparative Example 1), with a proliferation rate between 105.0% and 110.0%, the effect is relatively limited. In Example 1, when the above four components were combined in the specific proportions of the present invention, the cell proliferation rate surged to 135.2%, which not only far exceeded the effect of using each component alone, but was also close to the level of the minoxidil positive control group. This indicates that each component produced a strong synergistic hair growth mechanism in targeting and activating hair follicle stem cells and improving the hair follicle microenvironment.

[0042] Furthermore, comparing Examples 1 with Examples 2-6, it can be seen that within the formulation range protected by this invention, the compositions all maintain good proliferative activity, with the formulation ratio of Example 1 exhibiting the best biological activity. More importantly, in Examples 7-10, when the composition ratio deviates significantly from the range defined by this invention, the cell proliferation rate drops sharply below the normal level, reaching a minimum of only 79.2%.

[0043] Comparing Examples 1 and 11-14, it is evident that the purity of St. John's wort flavonoids has a certain impact on the overall activity of the composition. In Example 11, when a commercially available crude extract of St. John's wort flavonoids with lower purity was used as a substitute, the cell proliferation rate decreased significantly, failing to achieve the optimal proliferation-promoting effect. In contrast, the test results of Examples 12, 13, and 14 show that when camellia seed oil, soybean seed extract, and hemp seed extract were replaced with common commercially available raw materials with lower characteristic purity, the cell proliferation rate of the composition only decreased slightly compared to Example 1.

[0044] 2. Hair growth factor expression detection Experimental objective: Vascular endothelial growth factor (VEGF), insulin-like growth factor-1 (IGF-1), and hepatocyte growth factor (HGF) are key positive nutrient factors regulating hair follicle microecology and the hair cycle. VEGF promotes angiogenesis around the hair follicle, providing sufficient blood supply and nutrition for hair development; IGF-1 plays a crucial role in prolonging the hair's anagen phase and delaying the entry of the hair follicle into the catagen phase; and HGF significantly stimulates the proliferation of hair follicle epithelial cells and induces hair follicle regeneration. This experiment aims to verify, at the molecular level of mRNA expression, whether the composition of this invention can synergistically upregulate the expression of these core growth factors.

[0045] Experimental Methods: Human papillary dermal cells (HDPC) were used, and the groups were the same as in Experiment 1 above. After treatment for 48 hours, cells were collected, total RNA was extracted, and the mRNA expression levels of vascular endothelial growth factor (VEGF), insulin-like growth factor-1 (IGF-1), and hepatocyte growth factor (HGF) were detected by real-time quantitative PCR (qRT-PCR). GAPDH was used as an internal control, and the relative expression levels were calculated.

[0046] The test results are as follows.

[0047]

[0048] The test results show that the single components in Comparative Examples 2-5 had limited effect on increasing the expression levels of VEGF, IGF-1, and HGF, only by 1.05-1.30 times. However, after specific compounding in Example 1, the expression levels of the three factors surged to 2.35, 2.10, and 2.25 times, respectively, highly approaching the levels of the minoxidil-positive group, confirming that the composition has a deep synergistic mechanism for promoting hair growth at the molecular level. Within the preferred ratio ranges of Examples 5-6 and 2-4, the expression levels of each factor remained within the effective range of 1.85-2.05 times and 1.62-1.84 times, respectively. In Examples 7-10, when the ratio deviated significantly, the expression levels dropped to 0.70-0.90 times, producing negative inhibition. Furthermore, the data shows that high-grade St. John's wort flavonoids are the core of the signaling pathway. In Example 11, the reduction in purity caused the expression level to drop significantly to 1.05-1.10 times. However, in Examples 12-14, when the other plant materials were replaced with ordinary commercially available specifications, the expression level remained stable at a high level of 1.90-2.20 times, demonstrating good formulation stability and universality.

[0049] 3.5 α-Reductase Activity Inhibition Experiment Experimental objective: 5α-Reductase is a key target enzyme in androgenetic alopecia (AGA), primarily responsible for converting testosterone in the scalp into the more potent dihydrotestosterone (DHT). High concentrations of DHT bind to androgen receptors in hair follicles, leading to irreversible miniaturization of the follicles, a significantly shortened anagen phase, and premature entry into the telogen phase, ultimately causing hair loss. This experiment aims to evaluate the direct inhibitory effect of the components and compositions of this invention on the catalytic activity of 5α-reductase, verifying whether it can block the excessive production of DHT at its source, thereby confirming, on a biochemical metabolic basis, the key efficacy of this invention in blocking the causes of hair loss and protecting hair follicles from androgen attack.

[0050] Experimental Methods: The in vitro 5α-reductase inhibition activity assay kit was used for testing. The reaction system contained testosterone (substrate), NADPH (coenzyme), and 5α-reductase derived from rat liver. Grouping was the same as in Experiment 1 (final concentration based on total composition, samples were diluted to 0.1%), with an additional finasteride positive control group (0.1%). After incubation at 37°C for 30 minutes, the amount of dihydrotestosterone (DHT) produced was detected by high-performance liquid chromatography (HPLC), and the 5α-reductase inhibition rate was calculated.

[0051] The test results are as follows.

[0052]

[0053] 4. Detection of Wnt / β-catenin signaling pathway activation Experimental objective: The Wnt / β-catenin signaling pathway is the core "control switch" governing hair follicle stem cell differentiation, initiation of hair follicle morphogenesis, and the induction and maintenance of the hair growth phase. GSK-3β plays a crucial negative regulatory role in this pathway; when inactivated by phosphorylation (forming p-GSK-3β), it prevents the degradation of β-catenin protein, leading to the accumulation of large amounts of free β-catenin within the cell and its translocation to the nucleus, thereby activating the transcription of a series of downstream hair growth target genes. This experiment aims to verify the hair growth and hair follicle activation efficacy of the composition of this invention by detecting the total protein expression level of β-catenin and the phosphorylation ratio of GSK-3β.

[0054] Experimental methods: Human dermal papillary cells (HDPC) were used, and the groups were the same as in Experiment 1 above. After 48 hours of treatment, total protein was extracted from the cells, and the expression levels of β-catenin protein and phosphorylated GSK-3β were detected by Western blotting. GAPDH was used as an internal control, and the relative expression levels were calculated.

[0055] The test results are as follows.

[0056]

[0057] Data from Experiments 3 and 4 show that the composition of this invention possesses the dual efficacy of "inhibiting the root cause of hair loss" and "activating the hair growth engine." Comparing Example 1 as a blank group with the individual effective components in Examples 1 and 2-5, it can be seen that the highest inhibition rate of any single component on 5α-reductase is only 15.0%, and the increase in the relative expression levels of β-catenin protein and p-GSK-3β is negligible. However, when the four components are combined in the unique proportions of this invention (Example 1), the inhibition rate of 5α-reductase jumps to 72.5%, and the expression level of β-catenin and the p-GSK-3β / GSK-3β ratio reach as high as 2.80 and 0.85, respectively, achieving a powerful intervention on the two core hair growth signaling pathways, confirming that the composition forms a strong molecular-level synergistic effect at a specific ratio. Furthermore, comparing Example 1 with Examples 2-6, it can be seen that within the ratio range protected by this invention, the composition exhibits certain intervention on both signaling pathways, with the ratio in Example 1 showing the best biological activity. Comparing the data from Example 1 with those from Examples 7-10, it can be seen that in Examples 7-10, 5α-reductase was not only not inhibited, but also showed a negative value. Furthermore, the expression levels of key proteins in the Wnt / β-catenin pathway were significantly lower than those in the untreated blank control. This indicates that the incompatibility can induce severe component antagonism, which in turn worsens the hair follicle microenvironment.

[0058] In Example 11, when low-purity St. John's wort flavonoid crude extract was used as a substitute, the 5α-reductase inhibition rate plummeted to 12.5%, and Wnt signaling pathway activation was stalled. In Examples 12-14, when camellia seed oil, soybean seed extract, and hemp seed extract were replaced with commercially available products of conventional purity, the 5α-reductase inhibition rate and the expression levels of various proteins only slightly decreased compared to Example 1, remaining stably within an extremely excellent efficacy range.

[0059] 5. Evaluation of human efficacy Methods: Forty healthy volunteers (26 men and 14 women, aged 25-50 years) with mild to moderate hair loss were randomly divided into two groups: a control group (n=20) used a basic formula without soybean seed extract and hemp seed extract (containing only camellia seed oil and St. John's wort flavonoids), and an experimental group (n=20) used the scalp serum prepared in Example 1. Daily application was performed for 12 weeks. Scalp examination was conducted using a trichoscopy at baseline, week 4, week 8, and week 12 to calculate hair density per unit area and the proportion of hair follicles in the anagen phase. Subjective evaluation questionnaires were also collected from the participants.

[0060]

[0061] The test results in the table above show that after 12 weeks of continuous use, the composition of this invention exhibits comprehensive anti-hair loss and hair regrowth effects. Objective evaluations show that volunteers experienced an average increase of 13.6% in hair density, a 14.7% increase in the proportion of hair follicles in the growth phase, and a 20.0% decrease in the proportion of vellus hair, all significantly better than the control group containing only camellia seed oil and St. John's wort flavonoids. In terms of subjective evaluation, 65.0% of subjects reported a reduction in hair loss, 55.0% observed new hair growth, and 70.0% experienced an improvement in scalp oiliness. Furthermore, the product showed good tolerability, with only 1.5% of reports of adverse reactions such as scalp redness and itching, indicating good safety.

[0062] The above are all modifications that can be made to this embodiment without contributing any inventive step, or solutions that clearly constitute technical teaching, after reading this specification. However, as long as they are within the scope of the claims of this invention, they should be protected by patent law.

Claims

1. A plant-derived anti-hair loss composition containing camellia seed oil, characterized in that, The product comprises the following components by weight: camellia seed oil: 50-95 parts; St. John's wort flavonoids: 1.9-5 parts; soybean seed extract: 0.5-10 parts; hemp seed extract: 0.5-12 parts; the weight ratio of camellia seed oil to St. John's wort flavonoids is (10-50):1; the weight ratio of soybean seed extract to hemp seed extract is (0.2-5):

1.

2. The plant-derived anti-hair loss composition containing camellia seed oil according to claim 1, characterized in that, By weight, it includes the following components: Camellia seed oil: 70-90 parts; St. John's wort flavonoids: 2-3 parts; Edamame seed extract: 1-6 parts; Cannabis seed extract: 2-8 parts.

3. The plant-derived anti-hair loss composition containing camellia seed oil according to claim 1, characterized in that, The St. John's wort flavonoids are flavonoid extracts obtained by extracting and purifying the whole herb of St. John's wort, wherein the total flavonoid content is not less than 50%.

4. The plant-derived anti-hair loss composition containing camellia seed oil according to claim 1, characterized in that, The camellia seed oil is a vegetable oil obtained by pressing or extracting the seeds of the camellia plant (Theaceae family), and its unsaturated fatty acid content is not less than 80%.

5. The plant-derived anti-hair loss composition containing camellia seed oil according to claim 1, characterized in that, The edamame seed extract is an extract obtained by water extraction or alcohol extraction and purification with macroporous resin from the seeds of edamame, with a total flavonoid content of not less than 10% and an oligosaccharide content of not less than 15%.

6. The plant-derived anti-hair loss composition containing camellia seed oil according to claim 1, characterized in that, The hemp seed extract is an extract obtained by supercritical CO2 extraction or solvent extraction of dried mature hemp seeds, with a total unsaturated fatty acid content of not less than 70%, a linoleic acid content of not less than 50%, and a linolenic acid content of not less than 15%.

7. The plant-derived anti-hair loss composition containing camellia seed oil according to claim 1, characterized in that, The plant-derived anti-hair loss composition containing camellia seed oil also includes cosmetically acceptable excipients, which include one or more of solvents, emulsifiers, thickeners, moisturizers, preservatives, and fragrances.

8. A method for preparing the plant-derived anti-hair loss composition containing camellia seed oil according to any one of claims 1-7, characterized in that, The process includes the following steps: mixing camellia seed oil with St. John's wort flavonoids, soybean seed extract, and hemp seed extract evenly, adding cosmetically acceptable excipients, and stirring and emulsifying at 25-50℃ to obtain the final product.

9. The use of the plant-derived anti-hair loss composition containing camellia seed oil as described in any one of claims 1-7 and / or the composition prepared by the method of preparing the plant-derived anti-hair loss composition containing camellia seed oil as described in claim 8 in the preparation of a topical preparation for preventing hair loss and promoting hair growth.

10. The application according to claim 9, characterized in that, The topical preparations include scalp serums, shampoos, conditioners, scalp care masks, and hair growth solutions.

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