A sunscreen composition of a biomimetic plant cuticle and use thereof

Abstract

The present application relates to a kind of bionic plant epidermis sunscreen composition and its application, the sunscreen composition includes: dicocoylethyl pentaerythritol distearyl alcohol citrate, candelilla wax, buckwheat seed extract and lactobacillus / algae extract fermentation product. Wherein dicocoylethyl pentaerythritol distearyl alcohol citrate and candelilla wax can simulate wax film in plant epidermis leaf, reflect ultraviolet, have excellent sunscreen performance, and promote the film uniformity and waterproofness of product, and both synergies in the efficacy on;Wherein buckwheat seed extract and lactobacillus / algae extract fermentation product can absorb ultraviolet and antioxidant, reduce the damage of ultraviolet to skin, and have the effect of anti-inflammatory, anti-allergic and repair skin barrier, and both synergies in the efficacy on. The above-mentioned four active ingredients cooperate synergistically, and the composition formed is coated on skin can simulate the excellent protection of plant epidermis to ultraviolet, while natural mildness does not stimulate.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology and relates to a sunscreen composition that mimics the epidermis of plants and its application. Background Technology

[0002] Prolonged exposure to ultraviolet (UV) radiation can damage the skin and DNA, increasing the risk of skin cancer and photoaging. As public awareness of photoaging deepens, photoprotection has become an important part of skin health management. Sunscreen plays a crucial role in preventing photoaging, skin cancer, and photodermatitis.

[0003] However, current sunscreens still face several limitations in practical use. The photoprotective ingredients in existing sunscreens are mainly divided into two categories: physical sunscreens and chemical sunscreens. Physical sunscreens exert their protective effect through multiple mechanisms, such as absorbing and reflecting ultraviolet rays, and are relatively safe, but they have problems such as leaving a white cast and feeling heavy on the skin. Chemical sunscreens absorb ultraviolet rays and convert them into heat energy, resulting in a lighter texture and less white cast after application. However, some ingredients may pose risks of skin penetration and endocrine disruption, raising public concerns about their safety.

[0004] To address these challenges, the market needs to develop innovative sun protection technologies to provide more efficient, safe, and environmentally friendly sun protection solutions. Summary of the Invention

[0005] In view of the shortcomings of the prior art, the purpose of this invention is to provide a sunscreen composition based on biomimetic plant epidermis and its application.

[0006] To achieve this objective, the present invention employs the following technical solution.

[0007] In a first aspect, the present invention provides a sunscreen composition that mimics the epidermis of a plant, the sunscreen composition comprising: pentaerythritol distearate citrate, candelilla wax, buckwheat seed extract, and fermentation product of lactobacillus / algae extract.

[0008] The photoprotection mechanism of plant leaves mainly lies in two important components of the plant epidermis: one is the epidermal wax film that forms on the surface, whose unique structure reflects ultraviolet rays; the other is the polyphenols and flavonoids that accumulate in the vacuoles of epidermal cells, which can absorb ultraviolet rays. These two components complement each other, allowing plants to adapt to prolonged ultraviolet radiation.

[0009] This invention develops a biomimetic plant epidermis-based sunscreen composition, wherein dicofol pentaerythritol distearate citrate and candelilla wax can mimic the wax film in plant epidermal leaves, effectively reflecting ultraviolet rays and exhibiting excellent sunscreen performance. Furthermore, they enhance the film-forming uniformity and water resistance of the sunscreen product, with both components synergistically enhancing this effect. Additionally, buckwheat seed extract and Lactobacillus / algae extract fermentation products help absorb ultraviolet rays and provide antioxidant effects, reducing UV damage to the skin and exhibiting anti-inflammatory, anti-allergic, and skin barrier-repairing effects. Again, these components synergistically enhance this effect.

[0010] The four ingredients—discoyl pentaerythritol distearate citrate, candelilla wax, buckwheat seed extract, and Lactobacillus / algae extract fermentation product—work together synergistically to enhance the effects of sunscreens. When applied to the skin, this composition mimics the excellent UV protection provided by plant epidermis. It is also naturally gentle and non-irritating, thus providing a more effective and gentle enhancement of the protective effect of sunscreen products.

[0011] Preferably, the sunscreen composition comprises, by weight parts: 5-30 parts of pentaerythritol distearate citrate, 1-15 parts of candelilla wax, 0.05-0.5 parts of buckwheat seed extract, and 0.01-0.2 parts of Lactobacillus / algae extract fermentation product.

[0012] Based on the potential interactions among the diacoyl pentaerythritol distearate citrate, candelilla wax, buckwheat seed extract, and fermentation products of Lactobacillus / algae extract, when these components meet the specific mass ratios mentioned above, they are more effective in enhancing the sun protection, soothing and repairing, and waterproof properties of the product.

[0013] The mass fractions of the dicocoyl pentaerythritol distearate citrate can be selected from 5 parts, 8 parts, 10 parts, 12 parts, 15 parts, 18 parts, 20 parts, 25 parts, 27 parts, 30 parts, etc.; the mass fractions of the candelilla wax can be selected from 1 part, 2 parts, 4 parts, 5 parts, 7 parts, 9 parts, 11 parts, 13 parts, 15 parts, etc.; the mass fractions of the buckwheat seed extract can be selected from 0.05 parts, 0.06 parts, 0.07 parts, 0.08 parts, 0.09 parts, 0.1 parts, 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, etc.; the mass fractions of the lactobacillus / algae extract fermentation product can be selected from 0.01 parts, 0.05 parts, 0.1 parts, 0.12 parts, 0.14 parts, 0.15 parts, 0.17 parts, 0.18 parts, 0.2 parts, etc.

[0014] The buckwheat seed extract used in this invention is more preferably obtained by the following preparation method: using buckwheat seeds as a fermentation substrate, inoculating with Lactobacillus plantarum, Lactobacillus casei, and Saccharomyces cerevisiae for two-stage fermentation, followed by alcohol extraction. This method is more effective in improving the sun protection and soothing repair properties of the product.

[0015] Preferably, the buckwheat seed extract is prepared by a method comprising the following steps.

[0016] Step 1: After crushing the buckwheat seeds, mix them with a carbon source and water, and then sterilize them to obtain a sterile culture medium.

[0017] Step 2: Inoculate the sterile culture medium with Lactobacillus plantarum and Lactobacillus casei cultures for primary fermentation to obtain the primary fermentation product; then inoculate the primary fermentation product with Saccharomyces cerevisiae cultures for secondary fermentation to obtain the secondary fermentation product.

[0018] Step 3: Filter the secondary fermentation product, extract the filter residue with alcohol, centrifuge the extract, collect the supernatant, concentrate the supernatant under reduced pressure and dry it to obtain buckwheat seed extract.

[0019] Preferably, the ratio of buckwheat seeds to water is 1:(10-15) g / mL, for example, 1:10 g / mL, 1:11 g / mL, 1:12 g / mL, 1:13 g / mL, 1:14 g / mL, 1:15 g / mL, etc.

[0020] Preferably, the carbon source is 2-5% of the buckwheat seed mass, such as 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, etc.

[0021] Preferably, the carbon source is selected from any one or a combination of at least two of glucose, fructose, arabinose, mannose, sucrose, lactose, or maltose.

[0022] Preferably, the buckwheat seeds are pulverized to 40-80 mesh, such as 40 mesh, 50 mesh, 60 mesh, 80 mesh, etc.

[0023] Preferably, the concentration of the *Lactobacillus plantarum* bacterial solution is 1 × 10⁻⁶. 7 -1×10 9 CFU / mL, for example 1×10 7 CFU / mL, 5×10 7 CFU / mL, 1×10 8 CFU / mL, 5×10 8 CFU / mL, 1×10 9 The inoculation amount is 5-10% of the volume of the sterile culture medium, such as 5%, 6%, 7%, 8%, 9%, 10%, etc.

[0024] Preferably, the concentration of the Lactobacillus casei culture is 1×10⁻⁶. 7 -1×10 9 CFU / mL, for example 1×10 7CFU / mL, 5×10 7 CFU / mL, 1×10 8 CFU / mL, 5×10 8 CFU / mL, 1×10 9 The inoculation amount is 5-10% of the volume of the sterile culture medium, such as 5%, 6%, 7%, 8%, 9%, 10%, etc.

[0025] Preferably, the yeast culture solution containing 1×10⁻⁶ cells / mL is used. 6 -1×10 7 CFU / mL, for example 1×10 6 CFU / mL, 3×10 6 CFU / mL, 5×10 6 CFU / mL, 8×10 6 CFU / mL, 1×10 7 The inoculation amount is 5-10% of the volume of the sterile culture medium, such as 5%, 6%, 7%, 8%, 9%, 10%, etc.

[0026] Preferably, the primary fermentation is carried out at 32-38℃ (e.g., 32℃, 33℃, 34℃, 35℃, 36℃, 37℃, 38℃, etc.) for 24-48 h (e.g., 24 h, 30 h, 36 h, 42 h, 48 h, etc.), and the secondary fermentation is carried out at 28-32℃ (e.g., 28℃, 29℃, 30℃, 31℃, 32℃, etc.) for 48-72 h (e.g., 48 h, 52 h, 56 h, 60 h, 64 h, 68 h, 72 h, etc.).

[0027] Preferably, the extraction reagent used in the alcohol extraction treatment is a 50-70% aqueous ethanol solution, such as 50%, 55%, 60%, 65%, 70%, etc.

[0028] The extraction reagent used in the alcohol extraction process is a 50-70% aqueous ethanol solution because, compared to aqueous ethanol solutions of other concentrations, the extracted product is more effective in improving the sun protection and soothing repair properties of the product.

[0029] Preferably, the ratio of the filter residue to the ethanol aqueous solution is 1:(10-20) g / mL, for example, 1:10 g / mL, 1:12 g / mL, 1:14 g / mL, 1:15 g / mL, 1:16 g / mL, 1:18 g / mL, 1:20 g / mL, etc.

[0030] Preferably, the alcohol extraction treatment is a reflux treatment or a hot extraction treatment.

[0031] In a second aspect, the present invention provides the use of the sunscreen composition according to the first aspect in the preparation of sunscreen products.

[0032] Preferably, the sun protection product includes sunscreen lotion, sunscreen cream, sunscreen gel, and sunscreen stick.

[0033] Thirdly, the present invention provides a sunscreen, wherein the sunscreen comprises, by weight percentage, 1-10% of the sunscreen composition described in the first aspect, 10-25% of the sunscreen agent, 1-5% of the emulsifier, 5-15% of the emollient, 5-15% of the moisturizer, 0.1-2% of the thickener, 0.1-1% of the antioxidant, and the balance being water.

[0034] The mass percentage of the sunscreen composition can be selected from 1%, 2%, 4%, 6%, 8%, 10%, etc.; the mass percentage of the sunscreen agent can be selected from 10%, 12%, 14%, 16%, 18%, 20%, 22%, 25%, etc.; the mass percentage of the emulsifier can be selected from 1%, 2%, 3%, 4%, 5%, etc.; the mass percentage of the emollient can be selected from 5%, 7%, 8%, 10%, 12%, 15%, etc.; the mass percentage of the moisturizer can be selected from 5%, 7%, 8%, 10%, 12%, 15%, etc.; the mass percentage of the thickener can be selected from 0.1%, 0.5%, 1%, 1.2%, 1.5%, 2%, etc.; the mass percentage of the antioxidant can be selected from 0.1%, 0.2%, 0.4%, 0.6%, 0.8%, 1%, etc.

[0035] Preferably, the sunscreen agent comprises any one or a combination of at least two of the following: diethylamino hydroxybenzoyl hexyl benzoate, ethylhexyl triazine, bis-ethylhexyloxyphenol methoxyphenyl triazine, p-methoxycinnamate isoamyl ester, disodium phenyl dibenzimidazole tetrasulfonate, ethylhexyl methoxycinnamate, phenyl benzimidazole sulfonic acid, zinc oxide, and titanium dioxide.

[0036] Preferably, the emulsifier comprises any one or a combination of at least two of Tween 60, cetearyl alcohol, cetearyl glucoside, cetearyl oleate, cetearyl phosphate potassium, PEG-10 polydimethylsiloxane, and lauryl PEG-9 polydimethylsiloxane ethyl polydimethylsiloxane.

[0037] Preferably, the emollient comprises any one or a combination of at least two of isododecane, polydimethylsiloxane, diisopropyl sebacate, caprylic / capric triglyceride, and natural plant oils.

[0038] Preferably, the moisturizer comprises any one or a combination of at least two of the following: 1,3-propanediol, 1,3-butanediol, 1,2-hexanediol, glycerin, dipropylene glycol, panthenol, sodium hyaluronate, trehalose, and β-glucan.

[0039] Preferably, the thickener comprises any one or a combination of at least two of xanthan gum, polyacrylate crosspolymer-6, guar gum, hydroxyethyl cellulose, carbomer, and polyacrylamide.

[0040] Preferably, the antioxidant includes p-hydroxyacetophenone.

[0041] All other unlisted point values ​​within the numerical range involved in this invention can be selected and are all within the protection scope of this invention, and will not be elaborated here.

[0042] Compared with the prior art, the present invention has the following beneficial effects.

[0043] This invention develops a biomimetic plant epidermis-based sunscreen composition. Dicocoyl pentaerythritol distearate citrate and candelilla wax mimic the wax film in plant epidermal leaves, effectively reflecting ultraviolet rays and exhibiting excellent sun protection performance. They also improve the film-forming uniformity and water resistance of sunscreen products, with both components synergistically enhancing their effects. Buckwheat seed extract and Lactobacillus / algae extract fermentation products help absorb ultraviolet rays and provide antioxidant effects, reducing UV damage to the skin. They also have anti-inflammatory, anti-allergic, and skin barrier-repairing effects, with both components synergistically enhancing their effects. The four components—dicocoyl pentaerythritol distearate citrate, candelilla wax, buckwheat seed extract, and Lactobacillus / algae extract fermentation products—work together synergistically. When applied to the skin, this composition mimics the excellent UV protection of plant epidermis while being natural, gentle, and non-irritating, thus more effectively and gently enhancing the protective effect of sunscreen products. Detailed Implementation

[0044] To further illustrate the technical means and effects of the present invention, the following describes the technical solution of the present invention in conjunction with preferred embodiments of the present invention. However, the present invention is not limited to the scope of the embodiments.

[0045] The source information of the raw materials used in the following examples and comparative examples is shown in Table 1 below.

[0046] Table 1

[0047]

[0048] Preparation Example 1: This preparation example provides a buckwheat seed extract, the preparation method of which is as follows.

[0049] (1) After crushing the buckwheat seeds to 60 mesh, mix them evenly with glucose and water and then sterilize them. The ratio of buckwheat seeds to water is 1:10 g / mL, and the glucose mass is 4% of the buckwheat seed mass, thus obtaining a sterile culture medium.

[0050] (2) Inoculate the sterile culture medium with 5% Lactobacillus plantarum bacterial solution (1×10⁻⁶). 8 CFU / mL, ATCC8014) and 5% Lactobacillus casei culture (1×10⁻⁶) 8 The primary fermentation product was obtained by inoculating the primary fermentation product with 10% of *Saccharomyces cerevisiae* culture (1×10⁻⁶ CFU / mL, ATCC393) under anaerobic conditions at 37℃ for 36 h. 7 The product was obtained by secondary fermentation at 30°C under aerobic conditions for 55 h (CFU / mL, ATCC32693).

[0051] (3) Filter the secondary fermentation product and reflux the filter residue with 60% ethanol aqueous solution for 1.5 h. The ratio of filter residue to ethanol aqueous solution is 1:12 g / mL. Centrifuge the extract, take the supernatant, concentrate the supernatant under reduced pressure and dry it to obtain buckwheat seed extract.

[0052] Preparation Example 2: This preparation example provides a buckwheat seed extract, the preparation method of which is as follows.

[0053] (1) After crushing the buckwheat seeds to 80 mesh, mix them evenly with sucrose and water and then sterilize them. The ratio of buckwheat seeds to water is 1:12 g / mL, and the mass of sucrose is 5% of the mass of buckwheat seeds. A sterile culture medium is obtained.

[0054] (2) Inoculate the sterile culture medium with 5% Lactobacillus plantarum bacterial solution (1×10⁻⁶). 9 CFU / mL, ATCC8014) and 5% Lactobacillus casei culture (1×10⁻⁶) 9 The primary fermentation product was obtained by inoculating the primary fermentation product with 10% of *Saccharomyces cerevisiae* culture (1×10⁻⁶ CFU / mL, ATCC393) under anaerobic conditions at 35℃ for 48 h. 6 The product was obtained by secondary fermentation at 28°C under aerobic conditions for 60 h (CFU / mL, ATCC32693).

[0055] (3) Filter the secondary fermentation product and reflux the filter residue with 70% ethanol aqueous solution for 1.5 h. The ratio of filter residue to ethanol aqueous solution is 1:10 g / mL. Centrifuge the extract, take the supernatant, concentrate the supernatant under reduced pressure and dry it to obtain buckwheat seed extract.

[0056] Preparation Example 3: This preparation example provides a buckwheat seed extract, the preparation method of which is as follows.

[0057] (1) After crushing the buckwheat seeds to 50 mesh, mix them evenly with glucose and water and then sterilize them. The ratio of buckwheat seeds to water is 1:15 g / mL, and the glucose mass is 3% of the buckwheat seed mass, thus obtaining a sterile culture medium.

[0058] (2) Inoculate the sterile culture medium with 5% Lactobacillus plantarum bacterial solution (1×10⁻⁶). 7 CFU / mL, ATCC8014) and 5% Lactobacillus casei culture (1×10⁻⁶) 7 The primary fermentation product was obtained by inoculating the primary fermentation product with 10% of *Saccharomyces cerevisiae* culture (1×10⁻⁶ CFU / mL, ATCC393) under anaerobic conditions at 33℃ for 42 h. 7 The product was obtained by secondary fermentation at 32℃ under aerobic conditions for 48 h (CFU / mL, ATCC32693).

[0059] (3) Filter the secondary fermentation product and reflux the filter residue with 50% ethanol aqueous solution for 2.5 h. The ratio of filter residue to ethanol aqueous solution is 1:15 g / mL. Centrifuge the extract, take the supernatant, concentrate the supernatant under reduced pressure and dry it to obtain buckwheat seed extract.

[0060] Preparation Example 4: This preparation example provides a buckwheat seed extract, the only difference between which is the preparation method and that of Preparation Example 1, except that the ethanol aqueous solution in step (3) is a 90% ethanol aqueous solution. Other conditions remain unchanged.

[0061] Preparation Example 5: This preparation example provides a buckwheat seed extract, the only difference between which is the preparation method and that of Preparation Example 1, except that the ethanol aqueous solution in step (3) is a 30% ethanol aqueous solution. Other conditions remain unchanged.

[0062] Preparation Example 6: This preparation example provides a buckwheat seed extract, the preparation method of which differs from that of Preparation Example 1 only in step (2): 10% Lactobacillus plantarum bacterial suspension (1×10⁻⁶) is inoculated into a sterile culture medium. 8 The primary fermentation product was obtained by anaerobic fermentation at 37℃ for 36 h (CFU / mL). Then, 10% of *Saccharomyces cerevisiae* culture (1×10⁻⁶ CFU / mL) was inoculated into the primary fermentation product. 7 The product was subjected to secondary fermentation (CFU / mL) at 30°C under aerobic conditions for 55 h to obtain the secondary fermentation product. Other conditions remained unchanged.

[0063] Preparation Example 7: This preparation example provides a buckwheat seed extract, the preparation method of which differs from that of Preparation Example 1 only in step (2): 10% Lactobacillus casei culture (1×10⁻⁶) is inoculated into a sterile culture medium.8 The primary fermentation product was obtained by anaerobic fermentation at 37℃ for 36 h (CFU / mL). Then, 10% of *Saccharomyces cerevisiae* culture (1×10⁻⁶ CFU / mL) was inoculated into the primary fermentation product. 7 The product was subjected to secondary fermentation (CFU / mL) at 30°C under aerobic conditions for 55 h to obtain the secondary fermentation product. Other conditions remained unchanged.

[0064] Preparation Example 8: This preparation example provides a buckwheat seed extract, the preparation method of which differs from that of Preparation Example 1 only in step (2): 5% Lactobacillus plantarum bacterial suspension (1×10⁻⁶) is inoculated into a sterile culture medium. 8 CFU / mL) and 5% Lactobacillus casei culture (1×10⁻⁶ CFU / mL) 8 Fermentation was carried out at 37°C under anaerobic conditions for 72 h to obtain the fermentation product; the fermentation product was then processed in step (3). Other conditions remained unchanged.

[0065] Preparation Example 9: This preparation example provides a buckwheat seed extract, the preparation method of which differs from that of Preparation Example 1 only in step (2): 10% of *Saccharomyces cerevisiae* suspension (1×10⁻⁶) is inoculated into a sterile culture medium. 7 Fermentation was carried out at 30°C under aerobic conditions for 72 h to obtain the fermentation product; the fermentation product was then processed in step (3). Other conditions remained unchanged.

[0066] Preparation Example 10: This preparation example provides a buckwheat seed extract, the preparation method of which differs from that of Preparation Example 1 only in step (2): 10% of *Saccharomyces cerevisiae* suspension (1×10⁻⁶) is inoculated into a sterile culture medium. 7 The primary fermentation product was obtained by secondary fermentation at 30℃ under aerobic conditions for 55 h (CFU / mL). Then, 5% *Lactobacillus plantarum* culture (1×10⁻⁶ CFU / mL) was inoculated into the primary fermentation product. 8 CFU / mL) and 5% Lactobacillus casei culture (1×10⁻⁶ CFU / mL) 8 The product was fermented at 37°C for 36 h under anaerobic conditions (CFU / mL) to obtain a secondary fermentation product; other conditions remained unchanged.

[0067] Example 1: This example provides a sunscreen composition, which is composed of the following components in parts by weight: 20 parts of dicocoyl pentaerythritol distearate citrate, 15 parts of candelilla wax, 0.5 parts of buckwheat seed extract from Preparation Example 1, and 0.1 parts of Lactobacillus / algae extract fermentation product.

[0068] Example 2: This example provides a sunscreen composition, which is composed of the following components in parts by weight: 25 parts of dicocoyl pentaerythritol distearate citrate, 10 parts of candelilla wax, 0.4 parts of buckwheat seed extract from Preparation Example 2, and 0.2 parts of Lactobacillus / algae extract fermentation product.

[0069] Example 3: This example provides a sunscreen composition, which is composed of the following components in parts by weight: 30 parts of dicocoyl pentaerythritol distearate citrate, 5 parts of candelilla wax, 0.5 parts of buckwheat seed extract of Preparation Example 3, and 0.05 parts of Lactobacillus / algae extract fermentation product.

[0070] Examples 4-10: This example provides seven sunscreen compositions. The only difference between the sunscreen compositions and those in Example 1 is that the buckwheat seed extract of Preparation Example 1 is replaced with the buckwheat seed extract of Preparation Examples 4-10 in equal parts by mass.

[0071] Comparative Example 1: This comparative example provides a sunscreen composition that differs from Example 1 only in that it lacks candelilla wax and its reduced mass fraction is allocated to the mass fraction of dicocoyl pentaerythritol distearate citrate.

[0072] Comparative Example 2: This comparative example provides a sunscreen composition that differs from Example 1 only in that it lacks dicocoyl pentaerythritol distearate citrate, and the reduced mass fraction of this is allocated to the mass fraction of candelilla wax.

[0073] Comparative Example 3: This comparative example provides a sunscreen composition that differs from Example 1 only in that it lacks the fermentation product of lactobacillus / algae extract, and the reduced mass fraction of the product is allocated to the mass fraction of buckwheat seed extract of Preparation Example 1.

[0074] Comparative Example 4: This comparative example provides a sunscreen composition that differs from Example 1 only in that it lacks the buckwheat seed extract of Preparation Example 1, and its reduced mass fraction is allocated to the mass fraction of the Lactobacillus / algae extract fermentation product.

[0075] Application Example 1: This application example 1 provides a sunscreen, the formula of which is shown in Table 2 below.

[0076] Table 2

[0077]

[0078] The preparation method is as follows: (1) Water, xanthan gum, polyacrylate cross-linked polymer-6, 1,3-propanediol, 1,3-butanediol, Tween 60, cetearyl alcohol, cetearyl glucoside, 1,2-hexanediol and p-hydroxyacetophenone are heated to 85°C and kept at the temperature for 10 min until completely dissolved to obtain system 1.

[0079] (2) Diisopropyl sebacate, hexyl diethylamino hydroxybenzoyl benzoate, ethylhexyl triazine, bis-ethylhexyloxyphenol methoxyphenyl triazine, and p-methoxycinnamate are mixed, heated to 85°C, and kept at that temperature to obtain system 2.

[0080] (3) Dissolve the sunscreen composition in system 2, then add it to system 1, turn on the homogenizer to 7000 rpm, continue for 8 minutes until fully mixed, cool down, and obtain the sunscreen.

[0081] Application Examples 2-10: Application Examples 2-10 provide nine sunscreens, the only difference between their formulations and Application Example 1 is that the sunscreen composition of Example 1 is replaced in equal amounts with the sunscreen composition of Example 2-10, while other conditions remain unchanged. The preparation method is the same as in Application Example 1.

[0082] Comparative Application Examples 1-4: Comparative Application Examples 1-4 provide four sunscreens, the only difference between their formulations and Application Example 1 is that the sunscreen composition of Example 1 is replaced in equal amounts with the sunscreen compositions of Comparative Examples 1-4, while other conditions remain unchanged. The preparation method is the same as in Application Example 1.

[0083] Comparative Application Example 5: Comparative Application Example 5 provides a sunscreen whose formulation differs from Application Example 1 only in that it lacks the sunscreen composition of Example 1, and the amount of each of the four sunscreen agents is increased by 30%, while other conditions remain unchanged. Its preparation method is the same as in Application Example 1.

[0084] Test Example 1: Security Test.

[0085] The testing method was as follows: Ninety eligible social volunteers, aged 20-40 years, were randomly divided into three groups. Application examples 1-10 and control examples 1-5 were also divided into three groups, each with five different sunscreen products. Each group of 30 participants simultaneously tried all five sunscreen products within their group. The test products were placed inside the spot treatment apparatus chamber, while the control chamber remained untreated. The spot treatment apparatus containing the test product was applied to the inner forearm of the participants using hypoallergenic adhesive tape, and gently pressed with the palm to ensure even application to the skin for 48 hours. Skin reactions were observed according to the criteria in Table 3 at 30 minutes, 24 hours, and 48 hours after removing the spot treatment apparatus.

[0086] Table 3

[0087]

[0088] The results showed that all 10 volunteers tested with the sunscreen products of Application Examples 1-10 and Comparative Application Examples 1-4 had negative reactions, while 2 volunteers tested with the sunscreen of Comparative Application Example 5 experienced grade 1 skin reactions. Combined with the data from Test Example 2, this indicates that the sunscreen composition involved in this invention can effectively reduce the amount of chemical sunscreen agents used while achieving the same level of sun protection effect, making the sunscreen safer and gentler, without causing adverse reactions such as skin irritation or sensitization, and also improving the skin feel of the sunscreen.

[0089] Test Example 2: Sunscreen Efficacy Evaluation Test.

[0090] The sun protection factor (SPF) of products prepared according to the test cases 1-10 and comparative application cases 1-5 in the "Cosmetic Safety Technical Specifications 2015 Edition" were determined.

[0091] The specific testing method is as follows: Reference standard: SPF value 16.1±2.4, prepared according to the standard formula of high SPF standard (P2) in the "Cosmetic Safety Technical Specifications" (2015 edition).

[0092] Participants: A total of 150 people were divided into 15 groups, with 4 men and 6 women in each group, aged 25 to 50 years, and meeting the criteria for voluntary participation.

[0093] Light source: Xenon arc lamp from a daylight simulator; all performance indicators meet the requirements of the testing specifications.

[0094] Test Method: The test was conducted according to the specific requirements of the "Cosmetic Safety Technical Specifications" (2015 edition). Subjects were placed in a prone position, and their backs were irradiated. The minimum erythema dose (MED) of the subject's skin to ultraviolet radiation was predicted 24 hours prior to the test, and the ultraviolet radiation dose was adjusted based on the prediction results for testing the test substance. On the day of the test, three points at least 30cm apart were first selected on the subject's back. 2 In normal skin areas, at (2.00±0.05) mg / cm² 2 Apply the test substance or control evenly to the above-mentioned area and wait 15 minutes for film formation. Then, select the irradiation dose according to the standard requirements and irradiate under three conditions: ① no test substance applied to the subject's skin; ② application of control; ③ application of test substance. Wait 24 hours, observe the experimental results, and record the MED values ​​under each of the three conditions.

[0095] SPF value calculation method: The SPF value of the test substance or control for protecting a single subject is expressed by the following formula.

[0096]

[0097] Individual SPF values ​​must be accurate to one decimal place. The arithmetic mean of the SPF values ​​of all subjects protected by the test substance is calculated, and the integer part is taken as the SPF value of the sample being tested. The results are shown in Table 4.

[0098] Table 4

[0099]

[0100] As shown in Table 4, compared with Comparative Examples 1-4, the sunscreen involved in this invention contains dicofarad pentaerythritol distearate citrate, candelilla wax, buckwheat seed extract, and Lactobacillus / algae extract fermentation product. The combination of these four ingredients, when applied to the skin, can mimic the excellent protective effect of plant epidermis against ultraviolet rays, thus more effectively enhancing the sun protection effect of the sunscreen.

[0101] Comparing the results of Example 1 and Comparative Example 5, it can be seen that the sunscreen composition involved in the present invention can achieve a sunscreen enhancement effect. While achieving similar or better sunscreen effects, it can also effectively reduce the amount of chemical sunscreen agents used, thereby improving the safety of sunscreen products.

[0102] Comparing the results of Example 1 with those of Examples 4-10, it can be seen that the preparation process of buckwheat seed extract in the sunscreen composition involved in this invention also has a certain impact on the sunscreen effect of the product.

[0103] Test Example 3: Waterproofing Test.

[0104] (1) Take the test samples from Application Examples 1-10 and Comparative Application Examples 1-5 and apply them to the rough surface of the PMMA plate. Then, use a finger wearing a latex medical finger cot to spread the sample evenly. The sample amount should be controlled at 2 mg / cm³. 2 The sample must not contain air bubbles. Then place the PMMA plate in an environment of 25℃ and 50% relative humidity for 15 minutes. After calibrating the UV-2000S ultraviolet transmittance analyzer, test the SPF value of each sample (9 measurement points for each sample, and take the average value) to obtain the initial in vitro SPF value of each sample.

[0105] (2) After the SPF value was measured before contact with water, the PMMA plate was immersed in deionized water at 30°C and stirred at 200 r / min for 40 min. The PMMA plate was then removed and dried until the water droplets on its surface disappeared. The SPF value was measured using a UV-2000S ultraviolet transmittance analyzer (9 points were taken for each sample, and the average value was taken). This is the SPF value after contact with water. The SPF value retention rate (%) was calculated.

[0106] SPF retention rate (%) = SPF value of sunscreen after contact with water / SPF value of sunscreen before contact with water × 100%.

[0107] The results are shown in Table 5.

[0108] Table 5

[0109]

[0110] As shown in Table 5, compared with Comparative Example 5, the sunscreen involved in this invention contains dicofarad pentaerythritol distearate citrate, candelilla wax, buckwheat seed extract, and Lactobacillus / algae extract fermentation product. The combination of these four ingredients not only improves the sun protection effect of the sunscreen, but also improves its waterproof effect.

[0111] Comparing the results of Example 1 with those of Comparative Examples 1-2, it can be seen that dicocoyl pentaerythritol distearate citrate and candelilla wax have a synergistic effect in improving the waterproof effect of sunscreen.

[0112] The applicant declares that the technical solution of this invention is illustrated by the above embodiments, but this invention is not limited to the above embodiments, that is, it does not mean that this invention must rely on the above embodiments to be implemented. Those skilled in the art should understand that any improvements to this invention, equivalent substitutions of raw materials for the products of this invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of this invention.

[0113] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details of the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solutions of the present invention, and these simple modifications all fall within the protection scope of the present invention. Furthermore, it should be noted that the various specific technical features described in the above embodiments can be combined in any suitable manner without contradiction. To avoid unnecessary repetition, the present invention will not further describe the various possible combinations.

Claims

1. A sunscreen composition mimicking plant epidermis, characterized in that, The sunscreen composition comprises: pentaerythritol distearate citrate, candelilla wax, buckwheat seed extract, and Lactobacillus / algae extract fermentation product.

2. The sunscreen composition according to claim 1, characterized in that, The sunscreen composition comprises, by weight parts: 5-30 parts of pentaerythritol distearate citrate, 1-15 parts of candelilla wax, 0.05-0.5 parts of buckwheat seed extract, and 0.01-0.2 parts of Lactobacillus / algae extract fermentation product.

3. The sunscreen composition according to claim 1, characterized in that, The buckwheat seed extract is prepared by a method comprising the following steps: Step 1: After crushing the buckwheat seeds, mix them with a carbon source and water, then sterilize them to obtain a sterile culture medium. Step 2: Inoculate the sterile culture medium with Lactobacillus plantarum and Lactobacillus casei cultures and carry out a primary fermentation to obtain the primary fermentation product; Then, inoculate the primary fermentation product with the liquid of *Saccharomyces cerevisiae* to carry out a secondary fermentation to obtain the secondary fermentation product. Step 3: Filter the secondary fermentation product, extract the filter residue with alcohol, centrifuge the extract, collect the supernatant, concentrate the supernatant under reduced pressure and dry it to obtain buckwheat seed extract.

4. The sunscreen composition according to claim 3, characterized in that, The ratio of buckwheat seeds to water is 1:(10-15) g / mL, and the mass of the carbon source is 2-5% of the mass of the buckwheat seeds. The carbon source is selected from any one or a combination of at least two of glucose, fructose, arabinose, mannose, sucrose, lactose or maltose; The buckwheat seeds are crushed to 40-80 mesh.

5. The sunscreen composition according to claim 3, characterized in that, The concentration of the *Lactobacillus plantarum* bacterial solution is 1×10⁻⁶. 7 -1×10 9 CFU / mL, the inoculation volume is 5-10% of the sterile culture substrate volume; The concentration of the Lactobacillus casei culture was 1×10⁻⁶. 7 -1×10 9 CFU / mL, the inoculation volume is 5-10% of the sterile culture substrate volume; The capsule-coated yeast culture 1×10 6 -1×10 7 CFU / mL, the inoculation amount is 5-10% of the volume of sterile culture medium.

6. The sunscreen composition according to claim 3, characterized in that, The primary fermentation is carried out at 32-38℃ for 24-48 h, and the secondary fermentation is carried out at 28-32℃ for 48-72 h.

7. The sunscreen composition according to claim 3, characterized in that, The extraction reagent used in the alcohol extraction process is a 50-70% aqueous ethanol solution; The ratio of the filter residue to the ethanol aqueous solution is 1:(10-20) g / mL; The alcohol extraction process is either reflux extraction or hot extraction.

8. Use of the sunscreen composition according to any one of claims 1-7 in the preparation of sunscreen products.

9. A sunscreen, characterized in that, The sunscreen comprises, by weight percentage: 1-10% of the sunscreen composition according to any one of claims 1-7, 10-25% of the sunscreen agent, 1-5% of the emulsifier, 5-15% of the emollient, 5-15% of the moisturizer, 0.1-2% of the thickener, 0.1-1% of the antioxidant, and the balance being water.

10. The sunscreen according to claim 9, characterized in that, The sunscreen agent includes any one or a combination of at least two of the following: diethylamino hydroxybenzoyl hexyl benzoate, ethylhexyl triazine, bis-ethylhexyloxyphenol methoxyphenyl triazine, p-methoxycinnamate isoamyl ester, disodium phenyl dibenzimidazole tetrasulfonate, ethylhexyl methoxycinnamate, phenyl benzimidazole sulfonic acid, zinc oxide, and titanium dioxide. The emulsifier includes any one or a combination of at least two of the following: Tween 60, cetearyl alcohol, cetearyl glucoside, cetearyl olive oil ester, cetearyl phosphate potassium, PEG-10 polydimethylsiloxane, and lauryl PEG-9 polydimethylsiloxane ethyl polydimethylsiloxane. The emollient includes any one or a combination of at least two of the following: isododecane, polydimethylsiloxane, diisopropyl sebacate, caprylic / capric triglyceride, and natural plant oils. The moisturizer includes any one or a combination of at least two of the following: 1,3-propanediol, 1,3-butanediol, 1,2-hexanediol, glycerin, dipropylene glycol, panthenol, sodium hyaluronate, trehalose, and β-glucan. The thickener includes any one or a combination of at least two of xanthan gum, polyacrylate crosspolymer-6, guar gum, hydroxyethyl cellulose, carbomer, and polyacrylamide. The antioxidants include p-hydroxyacetophenone.

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