A temperature self-regulating cooling wet wipe based on heat-sensitive liposomes and a preparation method thereof

Abstract

The application provides a temperature self-adjusting cooling wet wipe based on heat-sensitive liposomes and a preparation method thereof. The wet wipe solution comprises, based on the total weight of the wet wipe solution, 2.0-4.0% of a cooling composition concentrate A, 0.2-2.0% of functional heat-sensitive liposomes B, and the balance of water. The functional heat-sensitive liposomes B comprise DPPC, DMPC and a fat-soluble active ingredient. The fat-soluble active ingredient comprises menthol. The wet wipe can intelligently sense the body surface temperature of a user, and automatically and accurately adjust the cooling release intensity and mode of the wet wipe according to the body surface temperature, so that the user can experience the experience of on-demand care of'strong cooling when hot and mild cooling when cool', and the effective action time is prolonged.

Description

Technical Field

[0001] This invention relates to the field of hygiene materials technology, and more specifically, to a temperature-regulating cooling wipe based on thermosensitive liposomes and its preparation method. Background Technology

[0002] In hot, humid, or high-intensity environments, the human body dissipates heat through sweating, often accompanied by discomfort such as burning and stickiness of the skin. Commercially available cooling wipes mostly rely on the instantaneous evaporation of high concentrations of alcohols (such as ethanol) or menthol in their formulas to provide a cooling sensation. However, this cooling sensation is singular and uncontrollable; its intensity is unrelated to the user's real-time perceived temperature. In cool environments such as air-conditioned rooms, an excessively strong cooling sensation can actually cause discomfort. Furthermore, cooling ingredients like menthol are volatile, leading to a decrease in product efficacy during storage and a short duration of cooling sensation during use.

[0003] Thermosensitive liposomes are a type of liposome that can operate at a specific temperature (phase transition temperature, T). m Nanoparticles undergo structural changes and rapidly release their contents under certain conditions. For example, encapsulating cooling active ingredients in thermosensitive liposomes allows for intelligent release of these active ingredients at their phase transition temperature. However, the phase transition temperature of thermosensitive liposomes in the pharmaceutical field is typically set above 41°C, far exceeding the temperature of human skin under natural conditions, making them untriggerable in everyday life. Furthermore, their release relies on external heating (such as laser irradiation of the skin), which contradicts the pursuit of convenient and immediate personal care procedures. Therefore, existing heat-triggered, externally dependent thermosensitive liposome technology cannot be directly used to respond to fluctuations in the body's own temperature.

[0004] Commercially available cooling wipes rely on the instantaneous evaporation of alcohol or high concentrations of menthol to provide a cooling sensation; or they use ordinary microcapsules (such as patent CN119498504A) to achieve sustained release, but lack temperature responsiveness. Existing cooling wipes have the following drawbacks: a. The cooling sensation is uncontrollable and disconnected from the environment: The intensity of its cooling sensation is fixed and does not adjust according to changes in the user's body temperature. In cool environments such as air-conditioned rooms, the excessive cooling sensation may cause discomfort; conversely, in truly hot environments, the cooling sensation may be insufficient. b. Insufficient duration: The cooling sensation, which relies on evaporation, is "intense and fleeting," failing to meet the need for sustained cooling during prolonged outdoor activities. The release of ordinary microcapsules is temperature-independent, and therefore cannot provide enhanced effects under heat stress. c. Lack of intelligent response mechanism: Existing technology cannot sense the temperature state of human skin, and its release is not "active" or "on demand", which does not match the actual thermal sensation of the human body.

[0005] Therefore, developing a wet wipe that can sense changes in the natural temperature of human skin and automatically and precisely adjust the release of cooling sensation to achieve intelligent on-demand release that is "strongly cooling when hot and mildly cooling when cool" has significant market value and application prospects.

[0006] In view of this, the present invention is hereby proposed. Summary of the Invention

[0007] This invention aims to overcome the shortcomings of existing cooling wipes, such as limited cooling sensation, uncontrollable cooling effect, poor durability, and incompatibility with usage scenarios. It provides a temperature-regulating cooling wipe based on thermosensitive liposomes and its preparation method. The wipe can intelligently sense the user's body surface temperature and adjust the intensity and mode of cooling release accordingly, achieving an on-demand care experience of "strong cooling when hot and gentle cooling when cool," while extending the effective action time.

[0008] In order to achieve the above-mentioned objectives of the present invention, the following technical solution is adopted: A temperature-regulating cooling wipe based on thermosensitive liposomes, wherein the wipe solution comprises, by weight of 100%, 2.0-4.0% of cooling composition concentrate A, 0.2-2.0% of functional thermosensitive liposomes B, and the remainder being water; The functional thermosensitive liposome B includes DPPC, DMPC, and a lipid-soluble active ingredient; The fat-soluble active ingredient includes menthol.

[0009] Furthermore, the mass ratio of DPPC to DMPC in the functional thermosensitive liposome B is 81:19-73:27.

[0010] Furthermore, the cooling composition concentrate A, based on a total weight of 100%, comprises 37.5-42.5% humectant a1, 6.5-8.5% solubilizer a2, 7.25-10% free cooling agent a3, with the balance being water.

[0011] Furthermore, the humectant a1 is at least one of propylene glycol, dipropylene glycol, and panthenol.

[0012] Furthermore, the solubilizer a2 includes at least one of PEG-40 hydrogenated castor oil and Tween 80.

[0013] Furthermore, the free cooling agent a3 includes at least two of WS-5, WS-10, WS-23, menthol amide, and menthol lactate.

[0014] Furthermore, the cooling composition concentrate A also includes: soothing ingredient a4 1.25-3.25%.

[0015] Furthermore, the cooling composition concentrate A also includes a preservative.

[0016] Furthermore, the soothing ingredient a4 includes at least two of the following: Centella asiatica extract, chamomile flower water, Sophora flavescens root extract, Tribulus terrestris fruit extract, and Cornus officinalis fruit extract.

[0017] Furthermore, the preservatives include: 1.5-3.5% chlorphenesin a5 and 0.5-2.5% benzalkonium chloride a6.

[0018] Furthermore, the wet wipe solution also includes 0.05-0.12% fragrance C.

[0019] Furthermore, the wet wipe solution also includes antibacterial agent D, which comprises: polyaminopropyl biguanide d1 0.3-0.5% and chlorhexidine gluconate d2 0.1-0.3%; wherein the polyaminopropyl biguanide is at an effective concentration of 20% and the chlorhexidine gluconate is at an effective concentration of 20%.

[0020] Furthermore, the mass of the fat-soluble active ingredient is 10-20% of the total mass of DPPC and DMPC.

[0021] Furthermore, the fat-soluble active ingredients also include clove bud oil and lime peel oil; the mass ratio of the fat-soluble active ingredients MT:ECBO:CADPO is 3:1:2.

[0022] The above-mentioned method for preparing temperature-regulating cooling wipes based on thermosensitive liposomes includes the following steps: Step S1: Prepare concentrated cooling composition solution A; Step S2: Prepare functional thermosensitive liposome B; Step S3: Mix the concentrated cooling composition A and the functional thermosensitive liposome B to prepare the wet wipe solution; Step S4: Wet wipes are formed.

[0023] Further, step S2 includes the following steps: Step 2.1: Dissolve DPPC, DMPC, and the fat-soluble active ingredient in the specified proportions; Step 2.2: Obtain a lipid film by vacuum evaporation; Step 2.3: Hydrate and swell the lipid membrane to obtain a liposome suspension; Step 2.4: The above liposome suspension is squeezed multiple times through a membrane with decreasing pore size under a constant temperature of 50℃~60℃. Step 2.5: Freeze and centrifuge the extruded liposome suspension, and freeze-dry the precipitate to obtain functional thermosensitive liposome B.

[0024] Compared with the prior art, the beneficial effects of the present invention are as follows: 1. This invention relates to a temperature-regulating cooling wipe based on thermosensitive liposomes, enabling intelligent temperature sensing and precise release: the core lies in self-made functional thermosensitive liposomes, whose phase transition temperature (T... m Designed to operate between 36℃ and 38℃, this temperature closely matches the body's surface temperature threshold for transitioning from comfort to heat stress. When wiping skin above this temperature, the liposome membrane undergoes a phase transition, rapidly releasing a high concentration of cooling ingredients, producing a "super cooling" sensation; below this temperature, the release is slower, providing only a mild cooling effect.

[0025] 2. This invention provides a temperature-regulating cooling wipe based on thermosensitive liposomes, which can achieve long-lasting cooling and sensory experience: the functional thermosensitive liposomes protect and slow-release volatile substances such as menthol, thus solving the problem of short duration of cooling sensation.

[0026] 3. This invention provides a temperature-regulating cooling wipe based on thermosensitive liposomes that combines efficacy and gentleness: the soothing ingredients in the formula (such as Centella Asiatica and Chamomile extracts) can relieve skin irritation caused by strong cooling. Attached Figure Description

[0027] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0028] Figure 1 Photograph of the functional thermosensitive liposome suspension prepared in Example 1 of this invention; Figure 2 The figure shows the experimental results of Experiment Example 2 of this invention; Figure 3 This is a diagram showing the experimental results of Experiment Example 3 of the present invention; Figure 4 This is a diagram showing the experimental results of Experiment Example 4 of the present invention; Figure 5 The figure shows the experimental results of Experiment Example 5 of this invention. Detailed Implementation

[0029] The technical solution of the present invention will be clearly and completely described below with reference to specific embodiments. However, those skilled in the art will understand that the embodiments described below are some embodiments of the present invention, but not all embodiments, and are only used to illustrate the present invention, and should not be regarded as limiting the scope of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall be followed. Where the manufacturers of reagents or instruments are not specified, they are all conventional products that can be purchased commercially.

[0030] A temperature-regulating cooling wipe based on thermosensitive liposomes, wherein the wipe solution comprises, by weight of 100%, 2.0-4.0% of cooling composition concentrate A, 0.2-2.0% of functional thermosensitive liposomes B, and the remainder being water.

[0031] In some embodiments, the cooling composition concentrate A, based on a total weight of 100%, comprises 37.5-42.5% humectant a1, 6.5-8.5% solubilizer a2, 7.25-10% free cooling agent a3, and the balance being water.

[0032] Furthermore, the humectant a1 is at least one of propylene glycol, dipropylene glycol, and panthenol.

[0033] Furthermore, the solubilizer a2 includes at least one of PEG-40 hydrogenated castor oil and Tween 80.

[0034] Further, the free cooling agent a3 includes at least two of WS-5 (N-(ethoxycarbonylmethyl)-p-oxo-3-methaneamide, CAS 68489-14-5), WS-10 (mentholoxypropylene glycol, CAS 87061-04-9), WS-23 (methyl diisopropylpropionamide, CAS 51115-67-4), mentholamide, and menthol lactate.

[0035] In some embodiments, the cooling composition concentrate A further includes: 1.25-3.25% of soothing ingredient a4, wherein soothing ingredient a4 includes at least two of Centella asiatica extract, chamomile flower water, Sophora flavescens root extract, Tribulus terrestris fruit extract, and Cornus officinalis fruit extract.

[0036] In some embodiments, the cooling composition concentrate A further includes preservatives, said preservatives comprising: 1.5-3.5% chlorphenesin a5 and 0.5-2.5% benzalkonium chloride a6.

[0037] In some embodiments, the wet wipe solution further includes 0.05-0.12% fragrance C.

[0038] In some embodiments, the wet wipe solution further includes an antibacterial agent D, which comprises: polyaminopropyl biguanide d1 0.3-0.5% (20% effective concentration) and chlorhexidine gluconate d2 0.1-0.3% (20% effective concentration).

[0039] In some embodiments, the functional thermosensitive liposome B includes DPPC (dispalmitoylphosphatidylcholine), DMPC (dismyristoylphosphatidylcholine), and a lipid-soluble active ingredient, with a DPPC to DMPC mass ratio of 81:19-73:27, including but not limited to 81:19, 80:20, 79:21, 78:22, 77:23, 76:24, 75:25, 74:26, and 73:27.

[0040] Further, the mass of the fat-soluble active ingredient is 10-20% of the total mass of DPPC and DMPC (including but not limited to 10%, 12%, 15%, 16%, 18%, and 20%), and the fat-soluble active ingredient includes menthol (MT), clove bud oil (ECBO), and lime peel oil (CADPO). A preferred mass ratio of MT:ECBO:CADPO is 3:1:2.

[0041] The above-mentioned method for preparing temperature-regulating cooling wipes based on thermosensitive liposomes includes the following steps: Step S1: Prepare concentrated cooling composition solution A; Step S2: Prepare functional thermosensitive liposome B; Step S3: Mix the cooling composition concentrate A and the functional thermosensitive liposomes B to prepare the wet wipe solution; Step S4: Wet wipes are formed.

[0042] In some implementations, step S2 includes the following steps: Step 2.1: Dissolve DPPC, DMPC, and the fat-soluble active ingredient together in a solvent in the specified proportions; Step 2.2: Evaporate under reduced pressure in a water bath at 40-45℃ (including but not limited to 40℃, 41℃, 42℃, 43℃, 44℃, 45℃) on a rotary evaporator to form a uniform lipid film co-loaded with active ingredients on the bottle wall; Step 2.3: Add PBS solution preheated to above 50°C to the container containing the lipid membrane, and stir at a constant temperature to hydrate and swell the lipid membrane; Step 2.4: The above liposome suspension is subjected to multiple extrusions through polycarbonate membranes with decreasing pore sizes (450nm, 220nm, 100nm) under constant temperature conditions of 50℃-60℃ (including but not limited to 50℃, 56℃, 58℃, 60℃). Step 2.5: Freeze and centrifuge the extruded liposome suspension, and freeze-dry the precipitate to obtain functional thermosensitive liposome B.

[0043] Example 1 A method for preparing temperature-regulating cooling wipes based on thermosensitive liposomes includes the following steps: 1. Prepare concentrated cooling composition solution A: a. In a heating apparatus, add 22.5% propylene glycol, 6.5% PEG-40 hydrogenated castor oil, 2.9% menthol amide, and 4.35% menthol lactate. Heat and stir at 45°C and 300 rpm for 30 minutes until the system is completely clear and transparent; b. While maintaining stirring and temperature, add the remaining 15% propylene glycol and 1.5% chlorphenesin to the clarified liquid, and continue stirring for 10 minutes; c. Stop heating, add 45.5% EDI water to the system, then add 0.5% benzalkonium chloride, 0.5% Centella asiatica extract, 0.5% chamomile flower water, and 0.25% Sophora flavescens root extract. Stir at 200 rpm for 10 minutes at room temperature to ensure all components are mixed evenly, and obtain a cool, concentrated solution for later use.

[0044] 2. Preparation of functional thermosensitive liposomes B: a. Dissolve DPPC and DMPC (mass ratio 77:23), along with the lipid-soluble active ingredients MT, ECBO, and CADPO (mass ratio 3:1:2, the total mass of which is 15% of the total mass of DPPC and DMPC), in chloroform. Evaporate under reduced pressure in a 40°C water bath using a rotary evaporator to completely remove the organic solvent, forming a uniform lipid film containing the co-loaded active ingredients on the bottle wall. b. Add PBS solution preheated to above 50°C to the container containing the lipid membrane, and stir at a constant temperature to hydrate and swell the lipid membrane to obtain a liposome suspension; c. The above liposome suspension was extruded multiple times through polycarbonate membranes with decreasing pore sizes (450nm, 220nm, 100nm) under a constant temperature of 55°C. d. Centrifuge the extruded liposome suspension at 1500g for 5 min in a refrigerated centrifuge at 4℃ to remove the precipitate, then centrifuge at 8000g for 10 min to remove the supernatant. Freeze the precipitate overnight in a freeze dryer (-50℃, 0.1 MPa), weigh it, and you will obtain functional thermosensitive liposome B, denoted as MT / ECBO / CADPO@DPPC / DMPC.

[0045] The functional thermosensitive liposome B was resuspended in pre-cooled PBS and the concentration was adjusted to 0.2 g / mL for later use.

[0046] 3. Preparation of wet wipe solution and wet wipe shaping a. Add 0.1% flavoring to 3.75% concentrated cooling composition A and stir for 10 minutes until the system is clear and transparent; b. Add 94.9% EDI water to the mixing tank and start stirring (200 rpm). Add the mixture obtained in step a one by one and stir for 10 minutes until homogeneous; c. Add 0.45% polyaminopropyl biguanide (20% effective concentration) and 0.3% chlorhexidine gluconate (20% effective concentration) sequentially, and stir for 15 minutes until completely dissolved; d. Adjust the stirring speed to 100 rpm, and slowly add 0.5% of the functional thermosensitive liposome B suspension along the wall. After adding, stir for 20 minutes to ensure uniform dispersion; e. Spray the final solution onto the folded spunlace nonwoven fabric, with each piece containing 3.4 times the solution. Then seal and package it with aluminum-plastic composite film.

[0047] Example 2 The difference between this embodiment and Embodiment 1 is that: The complete formula for the wet wipe solution is as follows: 3.0% cooling composition concentrate, 0.05% fragrance, 0.5% polyaminopropyl biguanide (20% effective concentration), 0.3% chlorhexidine gluconate (20% effective concentration), 1.0% functional thermosensitive liposomes, and the balance being water. The cooling composition concentrate consists of: 40% propylene glycol, 0.5% panthenol, 7.5% PEG-40 hydrogenated castor oil, 0.5% WS-5, 2.5% WS-10, 4.5% menthol lactate, 1% centella asiatica extract, 1% chamomile flower water, 2% chlorphenesin, 1.25% benzalkonium chloride, and the balance being water.

[0048] The mass ratio of DPPC to DMPC in functional thermosensitive liposomes is 81:19.

[0049] Example 3 The difference between this embodiment and Embodiment 2 is that the mass ratio of DPPC:DMPC in the functional thermosensitive liposomes is 77:23. Everything else is the same as in Embodiment 2.

[0050] Example 4 The difference between this embodiment and Embodiment 2 is that the mass ratio of DPPC:DMPC in the functional thermosensitive liposomes is 73:27. Everything else is the same as in Embodiment 2.

[0051] Comparative Example 1 The difference between Comparative Example 1 and Example 2 is that Comparative Example 1 does not contain functional heat-sensitive liposomes, but contains free cooling agent and unencapsulated menthol (in the same amount as the liposome-encapsulated menthol in Example 2). Everything else is the same as Example 2.

[0052] Comparative Example 2 The difference between Comparative Example 1 and Example 2 is that Comparative Example 1 does not contain functional thermosensitive liposomes and free cooling agents. Otherwise, it is the same as Example 2.

[0053] Experimental Example 1 The optimal ratio of menthol (MT), clove bud oil (ECBO), and lime peel oil (CADPO) among the fat-soluble active ingredients was determined. From various combinations of menthol, clove bud oil, and lime peel oil, sensory evaluation was used to select the aroma ratio with the highest overall preference for subsequent encapsulation with thermosensitive liposomes.

[0054] Experimental steps: 1. The mass ratio of MT:ECBO:CADPO is set as follows: 2:1:1, 3:1:2, 3:2:3, 4:1:4, and 4:2:4. Dissolve each ingredient in anhydrous ethanol to prepare a clear solution with a total concentration of 1%. 2. Fifteen evaluators with normal sense of smell were recruited and five samples were blindly rated using a 5-point Likert scale (1-5 points). The average score was used as the average preference. The experimental results are shown in Table 1.

[0055] Table 1. Results of Sensory Evaluation Experiment

[0056] Experimental conclusion: Based on the combined sensory evaluation and statistical analysis results, the optimal mass ratio of aroma components in the fat-soluble active ingredients was determined to be MT:ECBO:CADPO = 3:1:2.

[0057] Experiment Example 2 The optimal mass ratio of DPPC to DMPC was determined to enable the intelligent triggering of a large release of encapsulated menthol (MT) at 36-38°C.

[0058] Experimental steps: 1. Mix DPPC and DMPC in different mass ratios (100:0, 85:15, 81:19, 77:23, 73:27, 70:30, 0:100) with a fat-soluble active ingredient (mass ratio MT:ECBO:CADPO = 3:1:2) accounting for 15% of the total mass of DPPC and DMPC, and dissolve in chloroform.

[0059] 2. The solvent was removed by rotary evaporation to form a lipid film, which was then hydrated in PBS buffer at 55°C and extruded to obtain homogeneous liposomes.

[0060] 3. Free active substances were removed by freeze centrifugation, and the liposomes were freeze-dried and redispersed into functional thermosensitive liposomes with different DPPC / DMPC ratios of 0.2 g / mL.

[0061] 4. A portion of the prepared functional thermosensitive liposomes were heated at 60°C to completely rupture them. After centrifugation, the supernatant was collected, and the concentration of menthol was determined by gas chromatography (GC). The total encapsulation amount of functional thermosensitive liposomes with different DPPC / DMPC ratios was calculated.

[0062] 5. Another portion of functional thermosensitive liposomes with the same DPPC / DMPC ratio were incubated in water baths at 30℃, 34℃, 37℃, 40℃, and 43℃ for 60 minutes each. They were then immediately cooled in an ice bath and centrifuged at high speed. The supernatant was collected and the amount of menthol released at each temperature was determined by GC. The release rate was calculated as follows: Release rate = m 释放 / m 包封 *100%.

[0063] Experimental results are as follows Figure 2 As shown, when the mass ratio of DPPC:DMPC is between 81:19 and 73:27, the prepared functional thermosensitive liposomes can achieve rapid and large-volume release of menthol in the core range of 36℃-40℃, especially 36℃-38℃, exhibiting excellent temperature responsiveness and achieving automatic regulation of cooling release. The release is enhanced when the skin temperature rises to the threshold range, and reduced when the skin temperature is at normal, i.e., when the threshold range is not reached.

[0064] Experimental Example 3 The menthol release curves of the wet wipe solutions prepared in Examples 2, 3, 4, and Comparative Example 1 at 37°C over time (60 min) were tested. The experimental results are as follows: Figure 3 As shown.

[0065] like Figure 3 As shown, when the DPPC:DMPC mass ratio in the functional thermosensitive liposomes is in the range of 81:19 to 73:27, and at 37℃ (threshold temperature), menthol is gradually released over time, with the highest release rate of 88.6% within 60 min; while the release rate of free menthol not encapsulated by thermosensitive liposomes is 100%.

[0066] Experiment Example 4 The release curves of the wet wipe solutions prepared in Examples 2 (DPPC:DMPC=81:19), 3 (DPPC:DMPC=77:23), and 4 (DPPC:DMPC=73:27) after 1-3 heating-cooling cycles are shown in the experimental results. Figure 4 As shown.

[0067] The single heating-cooling release curve test method is as follows: after bathing in a 37℃ water bath for 10 minutes, the product is removed and the release rate is continuously measured within 10 minutes.

[0068] like Figure 4 It can be seen that the functional thermosensitive liposomes (DPPC:DMPC mass ratio of 81:19 to 73:27) in the wet wipe solutions prepared in Examples 2, 3 and 4 have good secondary release performance during heating and cooling cycles.

[0069] Experimental Example 5 The effective cooling time was tested using the following method: a. Five subjects were outdoors at 38°C for at least 30 minutes; b. Randomly select three test points on the subject's neck area and measure the initial temperature of the three points using a temperature gun; c. The wet wipes prepared in Examples 2-4 and Comparative Examples 1-2 were respectively placed on the necks of 5 subjects (i.e., Person 1 in Example 2, Person 2 in Example 3, Person 3 in Example 4, Person 4 in Comparative Example 1, and Person 5 in Comparative Example 2). After a certain period of time, the wet wipes were removed, and the temperature of the 3 points covered by the wet wipes was immediately recorded with a thermal imager. Then the wet wipes were hung up again. d. Repeat this step to continuously record the surface temperature of the skin covered by the wet wipe after different usage times, until the wet wipe coverage time reaches 1 hour and the experiment is stopped. Experimental results are as follows: Figure 5 As shown.

[0070] Depend on Figure 5 It can be seen that the addition of functional thermosensitive liposomes in Examples 2-4 maintained a good cooling effect even after 30 minutes. In contrast, the formulation in Comparative Example 1 contained only a free cooling agent, and after 30 minutes, the body temperature of the test subjects gradually increased due to the evaporation and inactivation of the cooling agent. The formulation in Comparative Example 2, which did not contain functional thermosensitive liposomes or a free cooling agent, showed no significant cooling effect, and the body temperature of the test subjects remained stable.

[0071] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features therein. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A temperature-regulating cooling wipe based on thermosensitive liposomes, characterized in that, The wet wipe solution, based on 100% of the total wet wipe solution weight, includes: 2.0-4.0% of cooling composition concentrate A, 0.2-2.0% of functional thermosensitive liposomes B, and the balance being water; The functional thermosensitive liposome B includes DPPC, DMPC, and a lipid-soluble active ingredient; The fat-soluble active ingredient includes menthol.

2. The temperature-regulating cooling wipes based on thermosensitive liposomes according to claim 1, characterized in that, The mass ratio of DPPC to DMPC in the functional thermosensitive liposome B is 81:19-73:

27.

3. The temperature-regulating cooling wipes based on thermosensitive liposomes according to claim 1, characterized in that, The cooling composition concentrate A, by total weight of 100%, comprises 37.5-42.5% humectant a1, 6.5-8.5% solubilizer a2, 7.25-10% free cooling agent a3, and the balance being water.

4. The temperature-regulating cooling wipes based on thermosensitive liposomes according to claim 3, characterized in that, It should include at least one of the following technical features: (1) The moisturizer a1 is at least one of propylene glycol, dipropylene glycol, and panthenol; (2) The solubilizer a2 includes at least one of PEG-40 hydrogenated castor oil and Tween 80; (3) The free cooling agent a3 includes at least two of WS-5, WS-10, WS-23, menthol amide, and menthol lactate; (4) The cooling composition concentrate A further includes: soothing ingredient a4 1.25-3.25%; (5) The cooling composition concentrate A also includes a preservative.

5. The temperature-regulating cooling wipes based on thermosensitive liposomes according to claim 4, characterized in that, It should include at least one of the following technical features: (1) The soothing ingredient a4 includes at least two of the following: Centella asiatica extract, chamomile flower water, Sophora flavescens root extract, Tribulus terrestris fruit extract, and Cornus officinalis fruit extract; (2) The preservatives include: 1.5-3.5% chlorphenesin a5 and 0.5-2.5% benzalkonium chloride a6.

6. The temperature-regulating cooling wipes based on thermosensitive liposomes according to claim 1, characterized in that, It should include at least one of the following technical features: (1) The wet wipe solution also includes 0.05-0.12% fragrance C; (2) The wet wipe solution also includes antibacterial agent D, which includes: polyaminopropyl biguanide d1 0.3-0.5% and chlorhexidine gluconate d2 0.1-0.3%; the polyaminopropyl biguanide is 20% effective concentration and the chlorhexidine gluconate is 20% effective concentration.

7. The temperature-regulating cooling wipes based on thermosensitive liposomes according to claim 1, characterized in that, The mass of the fat-soluble active ingredient is 10-20% of the total mass of DPPC and DMPC.

8. The temperature-regulating cooling wipes based on thermosensitive liposomes according to claim 7, characterized in that, The fat-soluble active ingredients also include clove bud oil and lime peel oil; the mass ratio of the fat-soluble active ingredients MT:ECBO:CADPO is 3:1:

2.

9. The method for preparing temperature-regulating cooling wipes based on thermosensitive liposomes as described in any one of claims 1 to 8, characterized in that, Includes the following steps: Step S1: Prepare concentrated cooling composition solution A; Step S2: Prepare functional thermosensitive liposome B; Step S3: Mix the concentrated cooling composition A and the functional thermosensitive liposome B to prepare the wet wipe solution; Step S4: Wet wipes are formed.

10. The method for preparing temperature-regulating cooling wipes based on thermosensitive liposomes according to claim 9, characterized in that, Step S2 includes the following steps: Step 2.1: Dissolve DPPC, DMPC, and the fat-soluble active ingredient in the specified proportions; Step 2.2: Obtain a lipid film by vacuum evaporation; Step 2.3: Hydrate and swell the lipid membrane to obtain a liposome suspension; Step 2.4: The above liposome suspension is squeezed multiple times through a membrane with decreasing pore size under a constant temperature of 50℃~60℃. Step 2.5: Freeze and centrifuge the extruded liposome suspension, and freeze-dry the precipitate to obtain functional thermosensitive liposome B.

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