A microecological balance deodorant composition based on a li plant composition and a preparation method and application thereof

Abstract

The present application provides a kind of microecological balance deodorant composition based on Lijia composition and its preparation method and application, and the active ingredient of the composition is composed of Hainan agarwood extract, sea Ba Jia fruit extract, white flower snake tongue grass extract, Guanghuoxiang extract, spearmint extract, Yizhi extract and Huangpi fruit kernel extract, is extracted by supercritical CO2, eutectic solvent extraction and ultrasonic assisted enzyme extraction three kinds of green process, no organic solvent residue in whole process, the composition also contains microecological balance factor and odor neutralizing factor, by directional inhibition of odor related bacterial flora excessive proliferation, while maintaining the balance of beneficial flora, and repairing skin barrier, realize the triple deodorization effect of microecological regulation, odor neutralization, barrier repair.The present application provides various dosage forms such as deodorant spray, walk pearl liquid, cream and foot care powder, process parameters are clear, equipment selection is clear, cost is controllable, and good industrialization feasibility is possessed.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and in particular to a microecological balancing deodorizing composition based on Li medicinal materials, its preparation method, and its application. Background Technology

[0002] Human body odor (especially armpit odor) mainly originates from unsaturated organic matter secreted by apocrine glands. After being decomposed by microorganisms colonizing the skin surface, it produces volatile odor-causing substances such as ammonia, hydrogen sulfide, thiols, and short-chain fatty acids (such as isovaleric acid). As consumers' demands for the safety and gentleness of skin care increase, the need for technological iteration in deodorant cosmetics is becoming increasingly prominent.

[0003] Currently, commercially available deodorizing cosmetics still have significant technical defects, mainly in the following two aspects: First, chemical antiperspirant products pose safety hazards. These products use aluminum salt antiperspirants such as aluminum hydroxychloride and aluminum zirconium chloride as their core active ingredients, achieving their deodorizing effect by blocking sweat gland ducts to reduce sweat secretion. However, long-term use of these ingredients can easily lead to problems such as clogged hair follicles, skin irritation, and clothing staining. Furthermore, their mechanism of action in inhibiting the body's normal perspiration function remains controversial regarding health and safety. Second, the extraction processes of existing plant-based deodorizing products are not environmentally friendly enough. Currently, most plant-based deodorizing products use petroleum-based organic solvents such as ethanol, acetone, and ethyl acetate to extract active ingredients. The production process generally carries high risks of solvent residue, large VOC emissions, and flammable and explosive safety hazards, which is inconsistent with the development trend of green chemistry and clean production.

[0004] In recent years, the correlation between skin microecological balance and skin barrier function has become a research hotspot in the field of skin care. Studies have confirmed that in the axillary skin microecological system, beneficial bacteria such as Staphylococcus epidermidis can inhibit the excessive proliferation of odor-causing microorganisms by competing for colonization sites and regulating the microenvironment; while the abnormal proliferation of odor-causing bacteria such as Staphylococcus aureus and Corynebacterium is the core microbiological cause of human odor. Accordingly, targeted regulation of the abundance of odor-causing bacteria in the armpit and maintenance of the homeostasis of beneficial skin flora, rather than broad-spectrum bactericidal action, has become the core research and development direction of the next generation of deodorant products.

[0005] Hainan Island boasts a unique tropical climate and abundant resources of distinctive medicinal plants. Among these, Hainan agarwood, Morinda officinalis (noni fruit), Hedyotis diffusa, patchouli, Alpinia oxyphylla, and Clausena lansium are commonly used in traditional Li medicine for clearing heat, reducing inflammation, and eliminating odors, possessing natural deodorizing potential. However, to date, existing technologies have not integrated these unique Hainan Li medicinal resources with the concept of skin microecological regulation, nor have they developed green extraction and preparation processes suitable for industrial production. There is a lack of systematic, green, and industrially feasible technical solutions for the deodorizing cosmetics field, making it difficult to meet market demand for safe, gentle, and environmentally friendly deodorizing products. Summary of the Invention

[0006] In view of this, the present invention proposes a microecological balancing deodorizing composition based on Li medicinal materials, its preparation method and application, to solve the above problems.

[0007] The technical solution of the present invention is implemented as follows: a microecological balancing deodorizing composition based on Li medicinal materials, comprising the following raw materials in the following mass percentages (Table 1): Table 1 Raw material components and mass percentage of the deodorizing composition

[0008] The composition of the present invention achieves deodorization through the following triple mechanism: (1) Targeted regulation of microecology: Extracts from seven Hainan-specific plants synergistically regulate the growth environment of odor-related microorganisms through different pathways. Sesquiterpenoids in Hainan agarwood essential oil regulate the cell membrane permeability of odor-related microorganisms; anthraquinones from Morinda officinalis interfere with the protein synthesis of odor-related microorganisms; flavonoids from Hedyotis diffusa affect the stability of microbial cell walls and regulate their metabolic activity; patchouli alcohol interferes with microbial energy metabolism; spearmint carvone regulates the cell membrane permeability of microorganisms; oxytetracyclines inhibit excessive formation of microbial biofilms; and alkaloids from the kernel of Prunus persica interfere with the metabolic pathways of odor-related microorganisms. Experiments show that the minimum control concentration of this composition against Staphylococcus aureus is 0.05-0.2 mg / mL, and the minimum control concentration against Staphylococcus epidermidis is as high as 2.5-10 mg / mL. The selective regulation index (defined as the ratio of the minimum control concentration of Staphylococcus epidermidis to the minimum control concentration of Staphylococcus aureus) is greater than 12.5, achieving targeted regulation of odor-related microorganisms without disrupting the microecological balance.

[0009] (2) Odor chemical neutralization: Zinc ricinoleate in the odor neutralizing factor can form stable complexes with odor molecules such as thiols and ammonia; the cyclodextrin-encapsulated plant polyphenol complex utilizes the cavity structure of β-cyclodextrin to encapsulate the polyphenols of Morinda officinalis, the flavonoids of Hedyotis diffusa and the alkaloids of the kernel of Prunus persica, continuously adsorbing and chemically neutralizing odor precursors such as short-chain fatty acids.

[0010] (3) Skin barrier repair: Microecological balancing factors (fructooligosaccharides, inulin) act as prebiotics, selectively promoting the proliferation of beneficial bacteria (such as Staphylococcus epidermidis and non-pathogenic strains of Propionibacterium acnes). Odor-related microorganisms lack enzyme systems to break down β-2,1 glycosidic bonds, while beneficial bacteria possess corresponding enzyme systems, thereby achieving selective nutrient supply and competitively inhibiting the colonization of odor-related microorganisms.

[0011] This invention targets the active ingredient characteristics of seven Hainan-specific plants, and the extraction process is as follows: (1) Hainan agarwood extract is obtained by supercritical CO2 extraction process with extraction pressure of 18-35MPa, extraction temperature of 35-50℃, extraction time of 2-3h, CO2 flow rate of 20-30L / h, separation vessel pressure of 6-8MPa and temperature of 30-40℃. The obtained agarwood essential oil has no organic solvent residue and can be directly used for cosmetic fragrance.

[0012] (2) The extracts of Morinda officinalis fruit and Alpinia oxyphylla were obtained by a low eutectic solvent extraction process. The low eutectic solvent consisted of choline chloride and glycerol in a molar ratio of 1:2-1:3. The extraction temperature was 50-70℃, the extraction time was 1-2h, and the liquid-to-solid ratio was 10:1-20:1 (mL / g). After extraction, the extracts were back-extracted with anhydrous ethanol, concentrated under reduced pressure, and freeze-dried to obtain the extracts of Morinda officinalis fruit.

[0013] (3) Extracts were obtained from Hedyotis diffusa, Pogostemon cablin, Spearmint and Wampee kernels by ultrasound-assisted enzymatic extraction process. The ultrasound-assisted enzymatic extraction process met the following conditions: ultrasound power of 200W~400W, enzymatic hydrolysis temperature of 40℃~55℃, pH of enzymatic hydrolysis system of 4.5~5.5, enzymatic hydrolysis time of 60min~120min, and the amount of compound enzyme added was 1%~3% of the total mass of the raw materials. The compound enzyme was composed of cellulase and pectinase in a mass ratio of 1:2~1:3. After enzymatic hydrolysis, the enzyme was inactivated at 90℃~95℃ for 10min. The filtrate was collected by centrifugation and filtration. After removing impurities by ceramic membrane microfiltration, the filtrate was successively vacuum concentrated and spray dried to obtain extract powder.

[0014] Preferably, the ultrasonic power is 300W, the enzymatic hydrolysis temperature is 45-50℃, the pH is 5.0, the enzymatic hydrolysis time is 90min, the mass ratio of cellulase to pectinase is 1:2.5, the amount of compound enzyme added is 2%, and the liquid-to-solid ratio is 15:1 (mL / g).

[0015] The application of a lycopene extract composition in the preparation of skin deodorant cosmetics, wherein the deodorant cosmetics are selected from underarm deodorant cosmetics, foot deodorant cosmetics, or intimate area care deodorant products, wherein: (1) The underarm deodorant cosmetic includes deodorant spray, deodorant roll-on, and deodorant cream, and the mass percentage of the composition is 5.0-20.0%; (2) The foot deodorizing cosmetic includes foot deodorizing spray, foot deodorizing powder, and foot care cream, and the mass percentage of the composition is 3.0-15.0%; (3) The intimate area care deodorizing product includes care spray and care wash, the composition has a mass percentage of 1.0-8.0%, and the product pH value is 4.0-5.5.

[0016] Compared with the prior art, the beneficial effects of the present invention are: This invention overcomes the technical limitations of traditional deodorizing products that rely on sweat suppression and broad-spectrum sterilization. Centered on skin microecological balance, it combines seven extracts of Hainan's unique Li medicinal herbs, microecological balancing factors, and odor-neutralizing factors to construct a triple-action system of "targeted microbial regulation - chemical odor neutralization - skin barrier repair," achieving root-cause long-lasting deodorization. The composition exhibits a selective regulation index of up to 43.8 against odor-causing bacteria such as Staphylococcus aureus, with minimal impact on beneficial bacteria resident on the skin. In human trials, the immediate deodorization rate reaches 96.7%, and the 8-hour long-lasting deodorization rate is 88.3%. Continuous use for four weeks reduces the abundance of odor-causing bacteria by 62.3% and increases the abundance of beneficial bacteria by 28.7%, significantly improving skin microbial diversity.

[0017] This invention targets the active properties of seven raw materials and matches them with three types of green preparation processes: supercritical CO2 extraction, eutectic solvent extraction, and ultrasound-assisted enzymatic method. The entire process does not use petroleum-based organic solvents such as ethanol and acetone, eliminating solvent residues, VOC emissions, and flammability and explosion hazards from the source. The eutectic solvent recovery rate reaches 87.3%, which is clean, environmentally friendly, and reduces production costs. The resulting extracts all meet the requirements of the "Cosmetic Safety Technical Specifications".

[0018] The formula eliminates aluminum salt antiperspirants such as aluminum hydroxyl chloride, so it does not interfere with the body's normal perspiration and completely avoids the drawbacks of traditional products such as clogged hair follicles, skin irritation, and clothing staining. The product has an irritation index of only 0.6 on a 3D skin model and zero allergies in 60 human patch tests, making it gentle and suitable for long-term use by people with sensitive skin.

[0019] This composition can be flexibly adapted to various dosage forms such as sprays, roll-ons, creams, and powders, covering diverse scenarios such as underarms, feet, and feminine care. The preparation process can be implemented using conventional cosmetic production equipment, with a single batch capacity of 500-2000 kg and a room temperature stability period of up to 18 months. It has strong industrial feasibility and provides a technical path for the high-value development of Hainan's unique Li medicine resources. Detailed Implementation

[0020] To better understand the technical content of this invention, specific embodiments are provided below to further illustrate the invention.

[0021] Unless otherwise specified, the experimental methods used in the embodiments of this invention are all conventional methods.

[0022] Unless otherwise specified, all materials and reagents used in the embodiments of this invention are commercially available.

[0023] Raw materials and reagents of this invention: Hainan agarwood (Aquilaria sinensis) was purchased from agarwood planting base in Hainan (Haikou and Wenchang). It was identified as resinous wood from the Aquilaria genus of the Thymelaeaceae family, with an oil content ≥10%. Fresh fruit of Morinda citrifolia L., fruit of Alpinia oxyphylla Miq., and kernels of Clausena lansium were all collected from production areas in Changjiang and Wanning, Hainan. Whole plants of Hedyotis diffusa Willd., Pogostemon cablin Benth., and Mentha spicata L. were all purchased from Hainan medicinal herb markets and identified. All raw materials were dried, pulverized, and passed through a 40-mesh sieve before use.

[0024] Fructooligosaccharides (purity ≥95%, Quantum Hi-Tech or Belgian Beneo), inulin (purity ≥90%, Belgian Beneo or Chilean Cosmos), zinc ricinoleate (cosmetic grade, German BASF or equivalent domestic), β-cyclodextrin (pharmaceutical grade, Shaanxi Senfu or equivalent domestic), cellulase (enzyme activity ≥10000U / g, Ningxia Xiasheng), pectinase (enzyme activity ≥5000U / g, Ningxia Xiasheng), choline chloride (food grade, Shandong Hanang), glycerol (cosmetic grade, Coconut Palm Group or equivalent domestic).

[0025] Example 1: Preparation of Hainan Agarwood Extract 50 kg of Hainan agarwood powder (passed through a 40-mesh sieve) was placed in a supercritical CO2 extraction apparatus (extraction vessel volume 100 L). The extraction pressure was set to 28 MPa, the extraction temperature to 46 °C, the CO2 flow rate to 25 L / h, the pressure and temperature of separation vessel I to 9 MPa and 42 °C, and the pressure and temperature of separation vessel II to 7 MPa and 35 °C, with an extraction time of 2.5 hours. The CO2 was condensed, compressed, and recycled, with a recycling rate ≥95%. The extracts from separation vessels I and II were collected and combined to obtain 945 g of pale yellow transparent essential oil, with an extraction rate of 1.89%.

[0026] GC-MS analysis revealed that the main components were sesquiterpenoids (83.96%) and 2-(2-phenylethyl)chromones (including 25.08% fusarium oleoresin, 2.33% β-linalool, and 3.79% linalool). Solvent residue detection (GC-ECD method): No petroleum ether, ethyl acetate, ethanol, or other organic solvent residues were detected, meeting the requirements of the "Cosmetic Safety Technical Specifications".

[0027] Example 2: Preparation of Morinda officinalis fruit extract 30 kg of dried Morinda officinalis fruit powder was added to 450 L of a choline chloride-glycerol eutectic solvent (molar ratio 1:2) in a multi-functional extraction vessel (500 L capacity, equipped with stirring and temperature control). Extraction was carried out at 60 °C for 1.5 h with stirring at 60 rpm. After extraction, the extract was pumped into a D101 macroporous resin column (φ500×3000 mm, resin packing 500 kg). The column was first washed with three column volumes of deionized water to remove DES, and then eluted with 40% ethanol (food grade, recyclable distillation). The eluent was collected. The eluent was concentrated under reduced pressure (vacuum degree -0.09 MPa, temperature 55 °C) to a relative density of 1.15-1.20, and then freeze-dried (cold trap temperature -50 °C, vacuum degree ≤20 Pa) to obtain 4.26 kg of brownish-brown extract, with an extraction rate of 14.2%.

[0028] HPLC analysis revealed that the main active ingredients were anthraquinone compounds (calculated as emodin, content ≥2.5%) and flavonoids (calculated as rutin, content ≥3.8%). DPPH free radical scavenging IC 50 The value was 28.82 μg / mL. DES recovery: The water eluent was concentrated under reduced pressure to recover the choline chloride-glycerol mixture. After adding a small amount of glycerol to adjust the ratio, it could be recycled, with a recovery rate of 87.3%.

[0029] Example 3: Preparation of Hedyotis diffusa extract Take 20 kg of dried Hedyotis diffusa powder and add it to 300 L of deionized water in an ultrasonic-assisted enzymatic hydrolysis tank (500 L volume, ultrasonic frequency 28 kHz, ultrasonic power density 80 W / L). Adjust the pH to 5.0 (using a food-grade citric acid / sodium citrate buffer system), add 400 g of a complex enzyme of cellulase and pectinase (mass ratio 1:2.5) (2% of the complex enzyme is added), and enzymatically hydrolyze at 48 °C for 90 min while simultaneously turning on ultrasonic assistance. After enzymatic hydrolysis, the temperature was raised to 92℃ for 10 min to inactivate the enzyme. The mixture was then centrifuged using a tubular centrifuge (GQ105 type, speed 16000 rpm) to remove residue. The supernatant was then microfiltered through a ceramic membrane (alumina material, pore size 0.2μm, membrane area 20m²) to remove impurities. The filtrate was then concentrated under vacuum (vacuum degree -0.09MPa, temperature 55℃) to a solid content of 25-30%, and then spray-dried (inlet air temperature 170℃, outlet air temperature 75℃) to obtain 1.84 kg of yellow-green extract powder, with an extraction rate of 9.2%.

[0030] The total flavonoid content was determined to be 5.98% (calculated as rutin) by ultraviolet-visible spectrophotometry. No organic solvents were used in the entire process.

[0031] Example 4: Preparation of extracts from patchouli, spearmint, wampee kernel, and Alpinia oxyphylla. Patchouli extract, spearmint extract, and wampee kernel extract were prepared using the same ultrasound-assisted enzymatic process as in Example 3, with the same optimal parameters as in Example 3. Alpinia oxyphylla extract was prepared using the same eutectic solvent process as in Example 2, with choline chloride-glycerol (molar ratio 1:2.5) as the DES, at an extraction temperature of 65℃ for 1.5 hours, and a liquid-to-solid ratio of 15:1 (mL / g). The yields and key indicators of each extract are shown in Table 2 below. Table 2. Preparation methods, yields, and key active ingredients of patchouli, spearmint, wampee kernel, and alpinia oxyphylla extracts.

[0032] Example 5: Preparation of cyclodextrin-encapsulated plant polyphenol complex 1.0 kg of Morinda officinalis fruit extract obtained in Example 2, 0.8 kg of Hedyotis diffusa extract obtained in Example 3, and 0.5 kg of Phellodendron chinense kernel extract obtained in Example 4 were dissolved in 20 L of deionized water at 50 °C. 3.0 kg of β-cyclodextrin was added, and the mixture was stirred at 50 °C in a stirred tank (with jacketed temperature control) for 2 h at a stirring speed of 80 rpm. After inclusion, the mixture was spray-dried (inlet air temperature 170 °C, outlet air temperature 75 °C) to obtain 5.1 kg of cyclodextrin-included plant polyphenol complex. The inclusion rate was determined by ultraviolet spectrophotometry (wavelength 280 nm), showing good release performance (24 h release rate ≤35%, 72 h release rate ≥85%).

[0033] Example 6: Formulation of a microecological balancing deodorizing composition The components were weighed according to the following mass percentages: Hainan agarwood extract (Example 1) 2.0%, Morinda officinalis fruit extract (Example 2) 1.5%, Hedyotis diffusa extract (Example 3) 1.0%, Patchouli extract (Example 4) 0.5%, Spearmint extract (Example 4) 0.5%, Alpinia oxyphylla extract (Example 4) 0.3%, Wampee kernel extract (Example 4) 0.2%, Fructooligosaccharides 2.0%, Zinc ricinoleate 1.5%, Cyclodextrin-encapsulated plant polyphenol complex (Example 5) 3.0%, 1,3-Butanediol 5.0%, Glycerin 3.0%, and deionized water balance.

[0034] Preparation method: (1) Add seven kinds of Hainan characteristic plant extracts and oligofructose to deionized water, and stir and dissolve them in a stainless steel stirring kettle (volume 200 with jacket heating) at 45°C for 30 minutes at a stirring speed of 100 rpm. (2) Zinc ricinoleate and cyclodextrin-encapsulated plant polyphenol complex were premixed and ground in a three-dimensional mixer (50L capacity) at 30°C for 25 min to obtain odor neutralizing factor premix; (3) Add the premix from step (2) to the solution from step (1) and homogenize and emulsify it for 15 min using a high-shear homogenizer (4000 rpm). (4) Add 1,3-butanediol and glycerol, and adjust the pH to 5.5-6.0 with food-grade citric acid / sodium citrate buffer; (5) After filtration through a 0.22μm microporous membrane (PES material, effective filtration area 0.5m²), the product is filled into a spray pump bottle (100mL PET bottle with 0.16mL / time fine mist pump head) to obtain the underarm deodorant spray.

[0035] Application Example 1: Preparation of Deodorizing Roll-On Lotion Weigh each component according to the following mass percentages: Hainan agarwood extract 1.5%, Morinda officinalis fruit extract 1.0%, Hedyotis diffusa extract 0.8%, Patchouli extract 0.4%, Spearmint extract 0.4%, Alpinia oxyphylla extract 0.2%, Wampee kernel extract 0.1%, Inulin 1.5%, Zinc ricinoleate 1.0%, Cyclodextrin-encapsulated plant polyphenol complex 2.5%, Hydroxypropyl methylcellulose (HPMC, viscosity 4000 mPa·s) 1.2%, Propylene glycol 5.0%, Deionized water balance.

[0036] Preparation method: (1) Disperse HPMC in propylene glycol and allow it to swell at room temperature for 30 min; (2) Dissolve the seven plant extracts and inulin in deionized water and stir at 45°C to dissolve; (3) Add the HPMC dispersion from step (1) to step (2) and stir until homogeneous; (4) Add zinc ricinoleate and cyclodextrin complex, and homogenize at 3000 rpm for 10 min using a high shear homogenizer; (5) Adjust the pH to 5.5, filter through a 0.22μm microporous membrane, and fill into roll-on bottles (50mL glass bottles with stainless steel rollers). This process is suitable for production in a standard cosmetic emulsification workshop, with a single batch capacity of 500-2000kg.

[0037] Application Example 2: Preparation of Foot Deodorant Powder Weigh each component according to the following mass percentages: Hainan agarwood extract (spray-dried and microencapsulated, gelatin-gum arabic wall material, core-to-wall ratio 1:2) 1.0%, Morinda officinalis fruit extract 0.8%, Hedyotis diffusa extract 0.5%, Patchouli extract 0.3%, Spearmint extract 0.3%, Alpinia oxyphylla extract 0.2%, Wampee fruit kernel extract 0.2%, Fructooligosaccharides 1.0%, Zinc ricinoleate 2.0%, Corn starch 40.0%, Talc (pharmaceutical grade, sterilized) balance.

[0038] Preparation method: The microcapsule powders of various plant extracts, zinc ricinoleate, fructooligosaccharides, corn starch, and talc are sequentially added to a V-type mixer (200L capacity) and mixed for 30 minutes. The mixture is then passed through a 200-mesh sieve (74μm aperture) and filled into powder boxes (30g / box). This process requires no emulsification equipment, only mixing and sieving equipment, making it suitable for production in powder workshops. A single batch capacity can reach 500kg.

[0039] Test Example 1: Microbial Ecosystem Targeted Regulation Activity Test The difference in minimum regulatory concentration (MRC) between odor-related microorganisms and beneficial bacteria in the composition of this invention (formulation of Example 6) was determined using the agar dilution method. The test strains and results are shown in Table 3. Table 3 Minimum control concentration and selective control index of microorganisms and beneficial bacteria

[0040] Selective regulation index = MRC (beneficial bacteria) / MRC (odor-associated microorganisms).

[0041] The results showed that the composition of the present invention has a highly selective regulatory effect on odor-related microorganisms (index > 12.5) while having minimal impact on beneficial bacteria, thus confirming the effectiveness of the microecological balance design.

[0042] Test Example 2: Evaluation of Human Body Deodorization Efficacy and Safety Sixty volunteers with moderate to severe axillary odor (aged 18-45 years, half male and half female) were randomly divided into three groups. The groups used the spray described in Example 6, a commercially available aluminum-containing deodorant (control group 1), and a blank control (pure water, control group 2), respectively. A double-blind controlled trial was conducted, with continuous use for 28 days. Evaluation indicators and results are shown in Table 4. Table 4 Evaluation Results of Human Body Deodorization Efficacy and Safety

[0043] The results show that the product of this invention is significantly superior to traditional aluminum salt products in terms of long-lasting deodorization and safety, and does not cause clothing staining.

[0044] Test Example 3: Verification of the Effect of Microecological Balance Analysis of changes in axillary microbiota structure in volunteers after 4 weeks of use of the product of this invention (Example 6) using 16S rRNA gene sequencing (sampling site: axillary apocrine sweat gland distribution area, swab sampling, Illumina MiSeq sequencing): The relative abundance of odor-related microorganisms (Staphylococcus aureus, Corynebacterium) decreased by 62.3%; the relative abundance of beneficial bacteria (Staphylococcus epidermidis, Propionibacterium) increased by 28.7%; and the Shannon Index increased by 15.4%. This demonstrates that the composition of the present invention can significantly improve the microecological structure of the underarm skin while effectively deodorizing.

[0045] Test Example 4: Safety and Stability Evaluation Safety testing was conducted according to the "Cosmetic Safety Technical Specifications" (2015 edition): 3D skin model (EpiDerm) TM Stimulation index SI=0.6 (no irritation); zero allergic reactions in 60 human patch tests; no irritation in eye irritation test (alternative method, BCOP method).

[0046] Stability testing: The spray from Example 6 was placed at 4°C, 25°C, and 40°C, and its appearance, pH, active ingredient content, and microbial indicators were tested periodically. Results showed that the stability period exceeded 24 months at 4°C; the stability period was 18 months at 25°C, with an active ingredient retention rate >92%; after 3 months of accelerated testing at 40°C, the appearance and microbial indicators were satisfactory, and the active ingredient retention rate was >85%, meeting the shelf-life requirements for cosmetics.

[0047] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A microecological balancing deodorizing composition based on Li medicinal herbs, characterized in that, The composition comprises the following raw materials in weight percentages: 0.5-5.0% Hainan agarwood extract, 0.3-3.0% Morinda officinalis fruit extract, 0.2-2.5% Hedyotis diffusa extract, 0.1-1.5% patchouli extract, 0.1-1.5% spearmint extract, 0.05-1.0% Alpinia oxyphylla extract, 0.05-1.0% Wampee fruit kernel extract, 0.5-5.0% microecological balancing factor, 1.0-8.0% odor neutralizing factor, and the balance being cosmetic base.

2. The composition according to claim 1, characterized in that, The microecological balance factor is one or more of fructooligosaccharides, inulin, and α-glucan oligosaccharides.

3. The composition according to claim 1, characterized in that, The odor neutralizing factor comprises 0.5-3.0% zinc ricinoleate and 0.5-5.0% cyclodextrin-encapsulated plant polyphenol complex, wherein the cyclodextrin-encapsulated plant polyphenol complex is formed by encapsulating at least two plant active ingredients from β-cyclodextrin containing polyphenols from Morinda officinalis fruit, flavonoids from Hedyotis diffusa, and alkaloids from the kernel of Prunus persica fruit.

4. The composition according to claim 1, characterized in that, The Hainan agarwood extract was obtained by supercritical CO2 extraction process, with an extraction pressure of 18-35 MPa, an extraction temperature of 35-50℃, an extraction time of 2-3 h, a CO2 flow rate of 20-30 L / h, and a separation vessel pressure of 6-8 MPa and a temperature of 30-40℃.

5. The composition according to claim 1, characterized in that, The sea morinda fruit extract and the alpinia extract were obtained by a eutectic solvent extraction process. The eutectic solvent consisted of choline chloride and glycerol in a molar ratio of 1:2 to 1:

3. The extraction temperature was 50-70℃, the extraction time was 1-2 hours, and the liquid-to-solid ratio was 10:1-20:1 (mL / g). After extraction, the sea morinda fruit extract and the alpinia extract were obtained by back-extraction with anhydrous ethanol, concentration under reduced pressure, and freeze-drying.

6. The composition according to claim 1, characterized in that, The extracts from *Hedyotis diffusa*, *Pogostemon cablin*, *Spearmintum sieboldii*, and *Clausena lansium* seeds were obtained using an ultrasound-assisted enzymatic extraction process. The ultrasound-assisted enzymatic extraction process met the following conditions: ultrasound power of 200W-400W, enzymatic hydrolysis temperature of 40℃-55℃, pH of the enzymatic hydrolysis system of 4.5-5.5, enzymatic hydrolysis time of 60min-120min, and the amount of compound enzyme added was 1%-3% of the total mass of the extracted raw materials. The compound enzyme was composed of cellulase and pectinase in a mass ratio of 1:2-1:

3. After enzymatic hydrolysis, the enzyme was inactivated at 90℃-95℃ for 10min. The filtrate was collected by centrifugation and filtration. After impurity removal by ceramic membrane microfiltration, the filtrate was successively vacuum concentrated and spray-dried to obtain the extract powder.

7. The composition according to claim 6, characterized in that, The process parameters for ultrasound-assisted enzymatic extraction are as follows: ultrasound power 300W, enzymatic hydrolysis temperature 45-50℃, pH 5.0, enzymatic hydrolysis time 90min, cellulase to pectinase mass ratio 1:2.5, compound enzyme addition amount 2%, and liquid-to-solid ratio 15:1 (mL / g).

8. A method for preparing the composition according to any one of claims 1-7, characterized in that, Includes the following steps: (1) Prepare extracts of various Hainan-specific plants separately; (2) Zinc ricinoleate and β-cyclodextrin-encapsulated plant polyphenol complex were mixed and ground in a three-dimensional mixer at 30-40℃ for 20-30 min to obtain odor neutralizing factor premix; (3) Add the plant extracts and microecological balance factors obtained in step (1) to purified water, and stir and dissolve them in a stirred tank at 40-50℃ for 20-40 minutes at a speed of 80-120 rpm. (4) Add the odor neutralizing factor premix from step (2) to the solution from step (3), homogenize and emulsify it at 3000-5000 rpm for 10-20 min using a high shear homogenizer, and adjust the pH to 5.0-6.

5. (5) Add cosmetic matrix, mix evenly, filter through 0.22μm microporous filter membrane, and fill into the container to obtain the microecological balance deodorizing composition.

9. The use of the Rehmannia glutinosa extract composition according to any one of claims 1-7 or the Rehmannia glutinosa extract composition prepared by the method of claim 8 in the preparation of skin deodorizing cosmetics.

10. The application as described in claim 9, characterized in that, The deodorizing cosmetics are selected from underarm deodorizing cosmetics, foot deodorizing cosmetics, or intimate area care deodorizing products.

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