Plant-derived oil control composition based on camellia seed oil, and preparation method and application thereof

Abstract

The present application relates to the field of cosmetics, and relates to a plant source oil control composition based on camellia seed oil and a preparation method and application thereof, the composition comprising: camellia seed oil 50-95 parts; lotus leaf peptide 0.5-5 parts; nano zinc oxide 0.5-10 parts; polygonum aviculare extract 0.1-8 parts; the weight ratio of camellia seed oil to lotus leaf peptide is (10-100):1; the weight ratio of nano zinc oxide to polygonum aviculare extract is (0.5-5):1. The preparation method comprises the following steps: uniformly mixing camellia seed oil, lotus leaf peptide and polygonum aviculare extract, uniformly adding nano zinc oxide, then adding cosmetically or pharmaceutically acceptable adjuvants, stirring and emulsifying, and obtaining the composition. The composition can be used for preparing cosmetics or external transdermal absorption preparations for inhibiting sebaceous secretion, reducing skin oil secretion, improving oily skin and / or converging pores. The present application realizes long-acting oil control by compounding camellia seed oil, small molecule lotus leaf peptide, nano zinc oxide and polygonum aviculare extract, synergistically inhibits 5alpha-reductase and lipid synthesis, and has effects close to chemical oil control ingredients; the composition is mild, stable, non-irritating to sensitive oily skin and refreshing.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and more specifically to a plant-derived oil-controlling composition based on camellia seed oil, its preparation method, and its application. Background Technology

[0002] Sebum secretion is an important physiological process for maintaining the normal barrier function of the skin. However, due to factors such as endocrine, environment, diet, or mental stress, sebaceous glands often become overactive. Excessive sebum secretion is the main cause of oily skin, enlarged pores, and a shiny face. At the same time, excessive oil buildup can easily lead to abnormal keratinization of the pilosebaceous duct, providing a breeding ground for Propionibacterium acnes, which in turn causes inflammatory skin problems such as pimples and acne, seriously affecting the skin health and appearance of the individual.

[0003] Currently, there are numerous oil-control skincare products on the market targeting oily skin, but existing solutions generally have many insurmountable flaws in practical applications: On the one hand, many oil-control products rely on high concentrations of surfactants, ethanol, or chemical exfoliants such as salicylic acid and fruit acids. These solutions achieve an apparent oil-removing effect by powerfully cleansing the sebum film or exfoliating the stratum corneum. However, long-term or frequent use of these irritating ingredients can easily cause skin stinging, redness, and peeling, and can also severely damage the skin's normal natural lipid barrier, even leading to a vicious cycle of "the more you control oil, the oilier it gets" or "oily on the outside, dry on the inside," failing to meet the needs of sensitive or fragile oily skin groups.

[0004] On the other hand, some products claiming to be gentle primarily use conventional powder-based excipients in an attempt to alleviate the oiliness of the skin's surface. However, these products typically have an extremely short onset time, often losing their effectiveness within a few hours of application, requiring frequent reapplication. Furthermore, the accumulation of large amounts of powder can easily clog pores or cause makeup to become patchy. In addition, some existing compositions claiming to contain plant-derived oil-controlling ingredients often suffer from weak efficacy and slow onset of action in practical applications. Moreover, to achieve a certain apparent effect, extremely high amounts are often required, which not only significantly increases formulation costs but also severely damages the stability of the cosmetic matrix and the skin feel.

[0005] In summary, existing oil-control products often struggle to balance "mildness and safety" with "significant and long-lasting oil-control efficacy." Developing a skincare composition that is both gentle and non-irritating to the skin barrier, while breaking through the efficacy limitations of existing conventional ingredients to achieve truly significant and long-lasting oil-control effects, is a long-standing and pressing technical challenge in the cosmetics and skincare industry. Summary of the Invention

[0006] To address the aforementioned problems, this invention provides a plant-derived oil-controlling composition based on camellia seed oil, its preparation method, and its application.

[0007] In a first aspect, the plant-derived oil-controlling composition based on camellia seed oil provided by the present invention adopts the following technical solution: The plant-derived oil-controlling composition based on camellia seed oil comprises, by weight, the following components: (a) Camellia seed oil: 50-95 parts; (b) Lotus leaf peptides: 0.5-5 parts; (c) Nano zinc oxide: 0.5-10 parts; (d) Polygonum aviculare extract: 0.1-8 parts; The weight ratio of camellia seed oil to lotus leaf peptide is (10-100):1; the weight ratio of nano zinc oxide to Polygonum aviculare extract is (0.5-5):1.

[0008] Further, by weight, it includes the following components: (a) Camellia seed oil: 70-90 parts; (b) Lotus leaf peptides: 0.7-3 parts; (c) Nano zinc oxide: 1-5 parts; (d) Polygonum aviculare extract: 0.5-4 parts.

[0009] Furthermore, the lotus leaf peptide is a small molecule active peptide with a molecular weight of less than 1000 Da; the average particle size of the nano zinc oxide is 10-100 nm, and its surface is coated with a hydrophobic modifier, with a water contact angle greater than 120°.

[0010] Furthermore, the lotus leaf peptide is prepared by a complex protease hydrolysis process, the preparation steps of which include: adding dried lotus leaf powder to purified water at a mass ratio of 1:10-1:15, extracting at 85-95℃ for 1.5-2.5 hours, filtering to obtain a crude extract of lotus leaf protein; adjusting the pH of the crude extract to 7.5-8.5, adding a complex protease with a substrate mass fraction of 0.8-1.5%, and isothermal hydrolysis at 45-55℃ for 3-5 hours; after hydrolysis, inactivating the enzyme at 92-98℃ for 10-20 minutes, cooling, centrifuging to obtain the supernatant; ultrafiltration of the supernatant through an ultrafiltration membrane with a molecular weight cutoff of 1000 Da, collecting the permeate and freeze-drying to obtain the lotus leaf peptide.

[0011] Furthermore, the complex protease is composed of papain and alkaline protease mixed in a mass ratio of 1:1.

[0012] Optionally, the hydrophobic modifier is selected from one or more of stearic acid, polydimethylsiloxane, hydroxyl-terminated polydimethylsiloxane, and triethoxyoctylsilane; the nano zinc oxide is prepared by reacting the nano zinc oxide with 2-5% by mass of the hydrophobic modifier in a mixture of isopropanol and anhydrous ethanol at 60-70°C under high shear coating conditions.

[0013] Furthermore, the camellia seed oil is a vegetable oil obtained by pressing or extracting the seeds of Camellia or Camellia oleifera, plants of the Theaceae family, wherein the mass fraction of unsaturated fatty acids is not less than 80% and the mass fraction of squalene is not less than 0.5%.

[0014] Furthermore, the Polygonum aviculare extract is an extract obtained by extracting the whole Polygonum aviculare plant with ethanol aqueous solution and purifying it with macroporous adsorption resin, wherein the mass fraction of total flavonoids is not less than 10% and the mass fraction of Polygonum aviculare glycosides is not less than 0.5%.

[0015] Secondly, the preparation method of the plant-derived oil-controlling composition based on camellia seed oil provided by the present invention adopts the following technical solution: The preparation method of the plant-derived oil-controlling composition based on camellia seed oil includes the following steps: Camellia seed oil is mixed evenly with lotus leaf peptide and Polygonum aviculare extract, nano zinc oxide is added and dispersed evenly, and then cosmetic or pharmaceutically acceptable excipients are added, and the mixture is stirred and emulsified to obtain the final product.

[0016] Thirdly, the application of the plant-derived oil-controlling composition based on camellia seed oil provided by this invention adopts the following technical solution: The above-mentioned plant-derived oil-controlling composition based on camellia seed oil is used in the preparation of cosmetics or topical transdermal absorption preparations for inhibiting sebum secretion, reducing skin oil production, improving oily skin and / or tightening pores.

[0017] In summary, the present invention has the following beneficial effects: 1. This invention combines camellia seed oil, specific small molecule (MW<1000Da) lotus leaf peptide, nano zinc oxide, and Polygonum aviculare extract in a specific ratio. The four components exhibit a significant synergistic effect in inhibiting 5α-reductase activity, blocking the root pathway of androgen-induced oil production, and inhibiting the lipid synthesis pathway of sebaceous gland cells. This composition breaks through the technical bottleneck of traditional single plant extracts having a single target, weak and slow onset of action. Its oil-controlling efficacy has jumped to a level close to that of specialized chemical oil-controlling drugs, achieving a truly significant and long-lasting oil-controlling effect. 2. This invention addresses the shortcomings of existing oil-controlling skincare products, which generally rely on high concentrations of surfactants, alcohol, or acidic chemical exfoliants, easily damaging the natural lipid barrier and causing "the more you control oil, the oilier it becomes" or "oily on the outside, dry on the inside." It constructs a highly safe, plant-based, gentle oil-controlling system. Human efficacy evaluations have confirmed that this composition not only significantly reduces sebum secretion in the T-zone of the face and tightens pores, but also has an adverse reaction rate of 0.0% during continuous use. While powerfully controlling oil, it maintains the stability of the skin's microecology, exhibiting extremely high friendliness and applicability to fragile, sensitive, and oily skin groups. 3. This invention not only gives skincare products an excellent immediate refreshing feel with a subject approval rate of over 95%, but it is also extremely easy to formulate and can be widely used in various cosmetic formulations such as oil-control essences, lotions, facial cleansers, and face creams, possessing extremely high industrial application and transformation value. Detailed Implementation

[0018] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. Reagents or instruments whose manufacturers are not specified are all conventional products that can be purchased commercially.

[0019] Camellia seed oil: Unsaturated fatty acid content ≥80%, squalene content ≥0.5%; sourced from Frog Prince (Fujian) Infant Care Products Co., Ltd.

[0020] Polygonum aviculare extract: total flavonoid content ≥1.5%, polysaccharide content ≥10%; sourced from Shanghai Jiayu Biotechnology Co., Ltd.

[0021] Lotus leaf peptide source:

[0022] It is obtained through the following preparation method: Step 1: Take 10 kg of dried and clean lotus leaf powder, put it into 120 L of purified water, turn on the stirrer, heat to 90℃, and extract continuously at a constant temperature for 2.0 hours. Use a microporous plate and frame filter press for cold filtration, collect the filtrate, and obtain the crude extract of lotus leaf protein. Step 2: Cool the filtrate to 50°C, precisely adjust the pH of the reaction system to 8.0 using a 10% NaOH solution, and then add a complex protease (which is a mixture of commercially available papain and alkaline protease in a 1:1 mass ratio) at 1.0% of the total protein mass of the substrate. Maintain a constant temperature of 50°C and stir for 4.5 hours for enzymatic hydrolysis. Step 3: After the enzymatic hydrolysis reaction is completed, the system is quickly heated to 95°C and kept at 95°C for 15 minutes to forcibly inactivate the enzyme. After cooling to room temperature, it is centrifuged at 4500 rpm for 20 minutes, the precipitate is discarded, and the clear supernatant is collected. Step 4: Pump the supernatant into the ultrafiltration membrane system, select a polyethersulfone (PES) ultrafiltration membrane with a specific molecular weight cutoff (MWCO) of 1000 Da for precision separation, carefully collect the permeate from the membrane, transfer it to a freeze dryer, and freeze dry it at -50°C and a vacuum degree of less than 10 Pa for 72 hours to finally obtain dry solid small molecule lotus leaf peptide powder.

[0023] Sources of nano zinc oxide:

[0024] Examples 1-6 The plant-derived oil-controlling composition based on camellia seed oil comprises, by weight, the following components:

[0025] It is prepared by the following method: The excipients include: emulsifier (a compound of cetearyl alcohol polyether-6 and cetearyl alcohol polyether-25), co-emulsifier (cetearyl alcohol), humectant (glycerin), thickener (xanthan gum), preservative (phenoxyethanol), and deionized water. (1) Add camellia seed oil, lotus leaf peptide and knotweed extract to the oil phase pot, and stir and mix for 15 minutes at 40°C and 200 rpm to make the lotus leaf peptide and knotweed extract evenly dispersed in the camellia seed oil to form an oil phase mixture. (2) Mix nano zinc oxide with an equal amount of camellia seed oil (reserved in the total amount of camellia seed oil in step (1)) and ultrasonically disperse for 10 minutes at 25°C and ultrasonic power of 100W to obtain a uniform nano zinc oxide pre-dispersion. (3) Add deionized water, glycerin and xanthan gum to the aqueous phase pot, heat to 80°C, stir for 20 minutes at 300 rpm to fully hydrate and swell the xanthan gum, and then cool to 40°C for later use. (4) Combine the oil phase mixture obtained in step (1) with the nano zinc oxide pre-dispersion obtained in step (2), stir evenly, and then slowly add it to the aqueous phase obtained in step (3). At the same time, add emulsifier and co-emulsifier, and stir and emulsify for 30 minutes at 40°C and 500 rpm to form a uniform emulsion. (5) Cool the emulsion obtained in step (4) to below 30°C, add preservatives, stir evenly, and discharge to obtain a plant-derived oil-controlling composition containing camellia seed oil.

[0026] Camellia seed oil is obtained by cold pressing and refining from Camellia oleifera seeds. Its unsaturated fatty acid content is 82.7% and squalene content is 0.58%. The Polygonum aviculare extract is a commercially available Polygonum aviculare extract (purchased from Shanghai Jiayu Biotechnology Co., Ltd.). The extract is obtained by extracting the whole Polygonum aviculare herb with 70% ethanol aqueous solution and purifying it with AB-8 macroporous adsorption resin. The total flavonoid content is 10.2% and the polygonum aviculare glycoside content is 0.51%, as determined by ultraviolet spectrophotometry and high performance liquid chromatography.

[0027] Comparative Examples 1-4 The preparation method of the plant-derived oil-controlling composition based on camellia seed oil is basically the same as that in Example 1. The only difference is that the weight ratio of camellia seed oil to lotus leaf peptide and the weight ratio of nano zinc oxide to Polygonum aviculare extract are different. The specific details are shown in the table below.

[0028]

[0029] Examples 7-13 The preparation method of the plant-derived oil-controlling composition based on camellia seed oil is basically the same as that in Example 1, except that the amount of each component is different, as shown in the table below.

[0030]

[0031] Comparative Examples 5-8 The preparation method of the plant-derived oil-controlling composition based on camellia seed oil is basically the same as that in Example 1, with the only difference being the use of lotus leaf peptides.

[0032]

[0033] Examples 14-16 The preparation method of the plant-derived oil-controlling composition based on camellia seed oil is basically the same as that in Example 1, except that the nano zinc oxide used is different, as detailed in the table below.

[0034]

[0035] Examples 17-18 The preparation method of the plant-derived oil-controlling composition based on camellia seed oil is basically the same as that in Example 1, except that the camellia seed oil used is different, as detailed in the table below.

[0036]

[0037] Example 19 The preparation method of the plant-derived oil-controlling composition based on camellia seed oil is basically the same as that in Example 1. The only difference is that the Polygonum aviculare extract used is different. In this example, another commercially available Polygonum aviculare extract is used. The total flavonoid mass fraction is 8.6% and the polygonum aviculare glycoside mass fraction is 0.37% as detected by ultraviolet spectrophotometry and high performance liquid chromatography.

[0038] Comparative Examples 9-15 A composition is prepared in a manner basically the same as in Example 1, with the only difference being the different uses of each component, as detailed in the table below.

[0039]

[0040] Application Example 1 An oil-controlling serum, based on a total weight of 100 parts, has the following composition:

[0041] It is prepared by the following method: 1. Disperse xanthan gum in a portion of deionized water and allow it to swell overnight to form a gel phase; 2. Add glycerin to the remaining deionized water and stir until homogeneous to form an aqueous phase; 3. The plant-derived oil-controlling composition prepared in Example 13 is mixed evenly with 1,2-pentanediol and p-hydroxyacetophenone, and stirred at high speed to disperse it evenly to form an oil phase; 4. Slowly add the oil phase to the aqueous phase while stirring, and homogenize and emulsify at high speed for 3-5 minutes; 5. Add the gel phase, stir well, and adjust the pH to 5.5-6.0 with citric acid.

[0042] Performance testing 1) Sebaceous gland cell lipid synthesis inhibition experiment Experimental Objective: The human sebaceous gland cell line (SZ95) is currently a standardized in vitro cell model used to evaluate the mechanisms of sebum secretion and regulation. Overactive sebaceous gland cells synthesize and secrete large amounts of neutral lipids, which is the root cause of oily skin, enlarged pores, and seborrheic dermatitis and acne. This experiment aims to directly quantify the inhibitory effect of the components and compositions of this invention on the synthesis and metabolism of neutral lipids in sebaceous gland cells using Oil Red O staining.

[0043] Experimental methods: The human sebaceous gland cell line (SZ95) was used for testing. Cells were seeded in 6-well plates, and samples prepared in the aforementioned examples and comparative examples were added respectively (final concentration based on the total amount of the composition, all samples were diluted to a concentration of 0.1%). After 48 hours of culture, the intracellular neutral lipid content was detected by Oil Red O staining, and the absorbance was measured at 490 nm to calculate the lipid synthesis inhibition rate. Inhibition rate (%) = (1 - OD value of sample group / OD value of blank control group) × 100%.

[0044] The test results are as follows.

[0045]

[0046] The test results above show that the lipid synthesis inhibition rates of Examples 1-13 remained at a high level. In contrast, when camellia seed oil (Comparative Example 10), lotus leaf peptide (Comparative Example 11), nano zinc oxide (Comparative Example 12), or Polygonum aviculare extract (Comparative Example 13) were used alone, the inhibition rates were only between 12.5% ​​and 22.4%, and the blank control (Comparative Example 9) showed no effect. This demonstrates that the four core ingredients are not simply physically mixed, but rather exhibit a significant synergistic effect, and the combination greatly enhances the activity of inhibiting lipid synthesis. By comparing the examples and comparative examples 1-4, it can be found that the "weight ratio of camellia seed oil to lotus leaf peptide" and the "weight ratio of nano zinc oxide to Polygonum aviculare extract" in the composition are very important. In comparative examples 2 and 4, when the ratio exceeds the preferred range of the present invention, the lipid synthesis inhibition rate drops sharply, falling to 22.1% and 18.3% respectively. Under the most suitable ratio in examples 7-13, the inhibition rate can be stabilized at over 82%. Comparative analysis of Example 16 reveals that when using unmodified hydrophilic nano-zinc oxide powder, the lipid synthesis inhibition rate drops to 61.7%. However, Examples 14 and 15, using modified nano-zinc oxide from different sources, show an inhibition rate rebounding to over 65%-68%; with the optimal formulation in Example 13, it reaches as high as 83.1%. This indicates that the hydrophobic treatment of the nano-zinc oxide surface prevents the nanoparticles from agglomerating in the system, improving their dispersibility and lipophilicity in the camellia seed oil matrix, making it easier for them to penetrate lipid-rich sebaceous gland ducts, thereby achieving better targeted synergistic effects with other plant active ingredients in the composition. Examples 17 and 18 investigated the impact of camellia seed oil quality differences on the final oil control effect. In Example 17, when the mass fraction of unsaturated fatty acids in camellia seed oil was less than 80% and the squalene content was less than 0.5%, its lipid inhibition rate dropped to 75.2%, lower than the 83.1% in Example 13. This indicates that high content of unsaturated fatty acids and squalene are excellent carriers for the system to exert high transdermal absorption and barrier repair functions. They can highly mimic the structure of human sebum membrane and carry lotus leaf peptides and Polygonum aviculare extract for deep penetration without damaging the skin's natural barrier. In Example 19, the content of total flavonoids (8.6%) and euryptic glycosides (0.37%) in the Polygonum aviculare extract was low, resulting in an inhibition rate of 75.6%. This indicates that the content of total flavonoids and euryptic glycosides has a certain effect on inhibiting 5α-reductase activity and blocking androgens. Meanwhile, the lipid synthesis inhibition rate of the optimal formulation of the present invention (Example 13) reached 83.1%, which means that the present invention, through the precise compounding of multi-target plant components, has achieved a mild oil-controlling efficacy close to that of oil-controlling drugs (Comparative Example 14) without relying on strong irritating chemical drugs, and has successfully achieved a perfect balance between "mild and safe" and "powerful oil control".

[0047] Comparative examples and data from Comparative Examples 5-8 show that the molecular weight of the selected lotus leaf peptides plays a crucial role in the oil-controlling effect. Unenzymatically hydrolyzed lotus leaf crude protein almost loses its oil-controlling ability, with an inhibition rate of only 9.4%. Even after enzymatic hydrolysis, if peptides with a molecular weight cutoff greater than 1000 Da are used, the inhibition rate can only hover around 42.6%. In contrast, the present invention uses small molecule oligopeptides with a molecular weight cutoff of less than 1000 Da, which significantly improves the efficiency of the active ingredients.

[0048] 2) 5α-Reductase Activity Inhibition Experiment Experimental Objective: 5α-Reductase is a key endocrine target enzyme regulating the activity of human sebaceous glands. In the physiological pathway of androgen-induced excessive lipid secretion, 5α-reductase is responsible for catalyzing the conversion of testosterone in the skin into highly reactive dihydrotestosterone (DHT). High concentrations of DHT specifically bind to androgen receptors within sebaceous gland cells, issuing strong instructions for lipid synthesis and proliferation, leading to excessive sebum secretion and subsequently causing problems such as oily skin, enlarged pores, and seborrheic dermatitis. This experiment aims to evaluate the direct inhibitory effect of the components and compositions of this invention on the catalytic activity of 5α-reductase.

[0049] Experimental methods: The in vitro 5α-reductase inhibitory activity assay kit was used for testing. The reaction system contained testosterone (substrate), NADPH (coenzyme), and 5α-reductase derived from rat liver. The aforementioned examples and comparative examples were added separately (final concentration based on the total amount of the composition; all samples were diluted to a concentration of 0.1%). After incubation at 37°C for 30 minutes, the amount of dihydrotestosterone (DHT) produced was detected by high performance liquid chromatography (HPLC), and the 5α-reductase inhibition rate was calculated.

[0050] The test results are as follows.

[0051]

[0052] The test results in the table above show that the single-component test data of Comparative Examples 10-13 indicate that the oil-controlling activity of camellia seed oil, lotus leaf peptide, nano zinc oxide, or Polygonum aviculare extract used alone is at a low level. However, in Examples 1-13 of this invention, after the above four components were specifically combined, both the lipid synthesis inhibition rate and the 5α-reductase inhibition rate were significantly increased. This proves that the core components of this invention have a good synergistic effect in inhibiting androgen conversion and blocking the lipid synthesis pathway, breaking through the technical bottleneck of single plant extracts having a single target and weak effect. Data from Example 16 shows that when using unmodified hydrophilic nano-zinc oxide powder, the composition already possesses a certain 5α-reductase inhibition capacity (46.8%). Building on this, Examples 14 and 15, using other hydrophobically modified nano-zinc oxide, showed an increasing inhibition rate (52.4% and 49.6%), indicating that hydrophobic modification has a positive auxiliary effect. Further comparison with Example 13 shows that when modified nano-zinc oxide is prepared using the preferred high-shear coating reaction of this invention, the inhibition rate is significantly improved. This suggests that this specific process can endow nanoparticles with superior spatial dispersibility and microscopic colonization ability, helping to assist in the precise delivery of active ingredients, thereby more fully leveraging the steric hindrance effect on the target enzyme. Examples 17 and 18 explored the effects of various indicators of camellia seed oil on the overall activity of the system. The data showed that even when the content of unsaturated fatty acids or squalene in camellia seed oil was low (as in Example 17), the system still maintained an effective inhibition rate of 56.8%. When the indicators met the preferred conditions of the present invention (unsaturated fatty acids ≥80%, squalene ≥0.5%) (as in Examples 18 and 13), the inhibitory efficacy of the system was further enhanced. This indicates that a high content of specific lipids can better exert the synergistic effect of "biomimetic transdermal" and is more compatible with the human sebum membrane, thereby effectively ensuring the concentration of active ingredients in the target area. In Example 19, when the total flavonoid and euryptic glycoside content of the Polygonum aviculare extract was adjusted to below the preferred lower limit, its 5α-reductase inhibition rate was 59.1%, which differed from the data of the optimal formulation. This result objectively indicates that when the concentrations of total flavonoids (≥10%) and euryptic glycosides (≥0.5%) reach this preferred range, the active molecules can better bind to the active site of 5α-reductase at the biochemical level, thereby obtaining a more ideal source-based oil-controlling biological efficacy.

[0053] Furthermore, the data from Comparative Examples 1-8 indicate that the weight ratio of the composition must be within the specific range specified in this invention; deviation from the ratio will lead to a significant decrease in synergistic effect. Moreover, the molecular weight of the lotus leaf peptide has a decisive influence on the overall activity; peptides that are not enzymatically hydrolyzed or have a molecular weight greater than 1000 Da cannot exert the desired efficacy. The preferred small molecule (MW < 1000 Da) oligopeptides of this invention can achieve more efficient transdermal penetration and generate sufficient steric hindrance to block the binding of the target enzyme. Meanwhile, by comparing with the positive control group (Comparative Example 15), it can be seen that the plant-derived oil-control composition of the present invention, with its natural and mild plant extract complex system, achieves an oil-control level close to that of chemical drugs.

[0054] 3) Evaluation of the body's oil-controlling effect Methods: Forty healthy volunteers with oily skin (26 women and 14 men, aged 18-40 years) were randomly divided into two groups: a control group (n=20) used a basic formula (containing only camellia seed oil and lotus leaf peptides) without nano-zinc oxide and Polygonum aviculare extract, and an experimental group (n=20) used the oil-controlling serum prepared in Example 1. Both were applied morning and evening for 8 consecutive weeks. At baseline, week 4, and week 8, sebum secretion in the T-zone (forehead and nose) was measured using a sebum meter, and subjective evaluation questionnaires were collected from the participants.

[0055]

[0056] As can be seen from the test results in the table above, after using the oil-controlling essence prepared in Application Example 1 of this invention for 8 consecutive weeks, the sebum secretion in the T-zone of the volunteers' faces was significantly suppressed. Specifically, the amount of sebum on the forehead of the experimental group decreased significantly by 37.0%, and the amount of sebum on the nose decreased by 36.0%; in contrast, the control group, which lacked nano-zinc oxide and Polygonum aviculare extract, showed only a slight improvement rate of 6.6% and 6.4% in the corresponding areas. This significant difference in data directly confirms, at the human clinical level, the strong synergistic effect among the four core components of this application, breaking through the bottleneck of weak oil-controlling effects of single plant extracts. Regarding subjective evaluations from test subjects, after 8 weeks of continuous use, the self-rated scores of the experimental group volunteers regarding skin oiliness decreased significantly by 28.3%; 68.0% of the subjects clearly agreed that the problem of enlarged pores was effectively improved; the immediate refreshing feeling score significantly increased by 92.9% (reaching 8.1 points); the overall oil control effect satisfaction rate was as high as 88.0%; more importantly, during the continuous testing period of 8 weeks, the incidence of adverse reactions in the experimental group remained at 0.0%, and no subjects experienced skin barrier damage such as redness, stinging, or peeling. This fully demonstrates that the plant-derived oil-control composition of the present invention achieves long-lasting and significant oil control effects while also possessing extremely high gentleness and safety.

[0057] The above are all modifications that can be made to this embodiment without contributing any inventive step, or solutions that clearly constitute technical teaching, after reading this specification. However, as long as they are within the scope of the claims of this invention, they should be protected by patent law.

Claims

1. A plant-derived oil-controlling composition based on camellia seed oil, characterized in that, By weight, it includes the following components: (a) Camellia seed oil: 50-95 parts; (b) Lotus leaf peptides: 0.5-5 parts; (c) Nano zinc oxide: 0.5-10 parts; (d) Polygonum aviculare extract: 0.1-8 parts; The weight ratio of camellia seed oil to lotus leaf peptide is (10-100):1; the weight ratio of nano zinc oxide to Polygonum aviculare extract is (0.5-5):

1.

2. The plant-derived oil-controlling composition based on camellia seed oil according to claim 1, characterized in that, By weight, it includes the following components: (a) Camellia seed oil: 70-90 parts; (b) Lotus leaf peptides: 0.7-3 parts; (c) Nano zinc oxide: 1-5 parts; (d) Polygonum aviculare extract: 0.5-4 parts.

3. The plant-derived oil-controlling composition based on camellia seed oil according to claim 1, characterized in that, The lotus leaf peptide is a small molecule active peptide with a molecular weight of less than 1000 Da; the average particle size of the nano zinc oxide is 10-100 nm, and its surface is coated with a hydrophobic modifier, with a water contact angle greater than 120°.

4. The plant-derived oil-controlling composition based on camellia seed oil according to claim 1, characterized in that, The camellia seed oil is a vegetable oil obtained by pressing or extracting the seeds of Camellia or Camellia oleifera, plants of the Theaceae family, wherein the mass fraction of unsaturated fatty acids is not less than 80% and the mass fraction of squalene is not less than 0.5%.

5. The plant-derived oil-controlling composition based on camellia seed oil according to claim 1, characterized in that, The Polygonum aviculare extract is an extract obtained by extracting the whole Polygonum aviculare plant with ethanol aqueous solution and purifying it with macroporous adsorption resin, wherein the mass fraction of total flavonoids is not less than 10% and the mass fraction of Polygonum aviculare glycosides is not less than 0.5%.

6. The method for preparing the plant-derived oil-controlling composition based on camellia seed oil according to any one of claims 1-5, characterized in that, The process includes the following steps: mixing camellia seed oil with lotus leaf peptide and Polygonum aviculare extract evenly, adding nano zinc oxide and dispersing evenly, then adding cosmetic or pharmaceutically acceptable excipients, stirring and emulsifying to obtain the final product.

7. The use of the plant-derived oil-controlling composition based on camellia seed oil according to any one of claims 1-5 in the preparation of cosmetics or topical transdermal absorption preparations for inhibiting sebum secretion, reducing skin oil production, improving oily skin and / or tightening pores.

8. The application according to claim 7, characterized in that, The cosmetics include serums, lotions, creams, cleansers, toners, and sprays.

9. The application according to claim 7, characterized in that, The topical transdermal absorption preparation includes scalp care solution.

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