Medicinal and edible alcohol-eliminating and liver-protecting compound composition, preparation method and application thereof

Abstract

The present application relates to the field of traditional Chinese medicine composition, disclose a kind of medicine and food homologous alcoholism liver protection compound composition and its preparation method and application.The medicine and food homologous alcoholism liver protection compound composition is made with jujube seed, mulberry leaf, jujube, eucommia leaf and tuckahoe as raw material, the amount of jujube seed is 6-12 weight parts, the amount of mulberry leaf is 5-8 weight parts, the amount of jujube is 2-9 weight parts, the amount of eucommia leaf is 2-10 weight parts, the amount of tuckahoe is 2-12 weight parts.The medicine and food homologous alcoholism liver protection compound composition of the present application can be used for preparing functional food or health care product for preventing acute alcoholism, relieving drunken symptoms, repairing alcoholic liver damage.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine composition technology, specifically to a compound composition for relieving hangovers and protecting the liver that is both food and medicine, its preparation method, and its application. Background Technology

[0002] Alcohol culture has been passed down for thousands of years in my country and is an important part of social life. While moderate drinking can promote blood circulation, excessive drinking can lead to acute alcohol poisoning, commonly known as drunkenness. Clinical manifestations include dizziness, nausea, loss of balance, agitation, abdominal distension, and irritability. In severe cases, it can cause respiratory distress and endanger life. From the perspective of traditional Chinese medicine, alcohol is considered to be damp and hot in nature. Excessive consumption can lead to the accumulation of alcohol toxins, obstructing the flow of Qi in the internal organs and damaging vital organs such as the liver, stomach, and kidneys. Long-term excessive drinking can induce irreversible liver damage.

[0003] The liver is the core organ for alcohol metabolism in the human body. After ethanol enters the body, it is mainly metabolized into acetic acid and water by alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) in the liver. Excessive drinking leads to a large accumulation of ethanol and acetaldehyde in the body. On the one hand, it paralyzes the central nervous system and causes symptoms of drunkenness. On the other hand, it produces a large amount of reactive oxygen species (ROS), which induces oxidative stress, inflammation, fatty degeneration, and even hepatocyte necrosis in liver cells. If it continues to develop, it will lead to liver diseases such as alcoholic fatty liver and alcoholic hepatitis.

[0004] Currently, most mainstream hangover relief and liver protection products on the market are chemical preparations, single peptides, or single traditional Chinese medicine ingredients, which have significant technical shortcomings: chemical drugs have significant toxic side effects, and long-term use can easily increase the metabolic burden on the liver and kidneys; single-ingredient products have a single target, only able to increase dehydrogenase activity or have mild anti-inflammatory effects, and cannot simultaneously achieve the multiple effects of rapid hangover relief, inhibition of alcohol accumulation, and repair of liver damage, and also have poor compatibility and unstable effects. Existing compound products mostly lack scientific compatibility and complete pharmacological experimental verification, making it difficult to guarantee the rationality of the formula and the stability of efficacy. Summary of the Invention

[0005] The purpose of this invention is to overcome the problems of existing hangover relief products, such as single efficacy, poor safety, lack of scientific compatibility, and weak liver damage repair ability, and to provide a compound composition for hangover relief and liver protection that is both food and medicine, as well as its preparation method and application.

[0006] To achieve the above objectives, the first aspect of the present invention provides a compound composition for relieving hangovers and protecting the liver, which is made from jujube seed, mulberry leaf, jujube, eucommia leaf and poria cocos. The amount of jujube seed is 6-12 parts by weight, the amount of mulberry leaf is 5-8 parts by weight, the amount of jujube is 2-9 parts by weight, the amount of eucommia leaf is 2-10 parts by weight and the amount of poria cocos is 2-12 parts by weight.

[0007] Preferably, the dosage form of the medicinal and edible compound for relieving hangovers and protecting the liver is powder, granules, tablets, oral liquid, or capsules.

[0008] A second aspect of this invention provides a method for preparing a compound composition for relieving hangovers and protecting the liver, comprising the following steps: (1) Soak the jujube seed, mulberry leaf, jujube, eucommia leaf and poria in water, decoct once, and then filter to obtain a filtrate and residue; (2) Boil the filter residue in water a second time, then filter it, collect the second filtrate, and combine the first filtrate and the second filtrate; The amount of jujube seed is 6-12 parts by weight, the amount of mulberry leaf is 5-8 parts by weight, the amount of jujube is 2-9 parts by weight, the amount of eucommia leaf is 2-10 parts by weight, and the amount of poria is 2-12 parts by weight.

[0009] Preferably, in step (1), during the soaking and the first decoction process, the amount of water used is 10-15 times the total weight of the jujube seed, the mulberry leaf, the jujube, the eucommia leaf and the poria.

[0010] Preferably, in step (1), the soaking time is 30-45 minutes.

[0011] Preferably, in step (1), the conditions for the first decoction include: a temperature of 95-100℃ at a gentle boil and a time of 1.5-2 hours.

[0012] Preferably, in step (2), the amount of water used in the secondary decoction process is 8-9 times the mass of the filter residue obtained in step (1).

[0013] Preferably, in step (2), the conditions for the second decoction include: a temperature of 95-100℃ at a gentle boil and a time of 1-1.5h.

[0014] Preferably, the method further includes: concentrating the combined filtrate by rotary evaporation under reduced pressure to obtain an extract, and then subjecting the extract to ultra-low temperature freezing and vacuum freeze-drying in sequence.

[0015] Preferably, the conditions for vacuum rotary evaporation concentration include: a temperature of 50-60℃, a vacuum negative pressure of 0.02-0.04MPa, and an absolute pressure of 60-80kPa.

[0016] Preferably, the conditions for ultra-low temperature freezing include: a temperature of -100°C to -50°C and a time of 24-36 hours.

[0017] The third aspect of this invention provides the application of the aforementioned food-medicine homology hangover relieving and liver-protecting compound composition or the food-medicine homology hangover relieving and liver-protecting compound composition prepared according to the aforementioned method in the preparation of functional foods or health products for preventing acute alcohol poisoning, relieving hangover symptoms, and repairing alcoholic liver damage.

[0018] According to the medicinal and edible homologous compound composition for relieving hangovers and protecting the liver described in this invention, the core active medicinal materials were screened based on network pharmacology, and the optimal ratio of the five medicinal materials—sour jujube seed, mulberry leaf, jujube, eucommia leaf, and poria cocos—was ultimately determined through a uniform design optimization method. In terms of relieving hangovers and protecting the liver, the compound composition of this invention has the advantages of strong targeting and reasonable compatibility, solving the problems of lack of scientific basis and unstable efficacy in existing compound compositions.

[0019] Furthermore, animal experiments have verified that the medicinal and edible compound composition for relieving hangovers and protecting the liver described in this invention has dual effects of rapid hangover relief and liver damage repair. It can significantly shorten the hangover relief time in intoxicated mice, accelerate alcohol metabolism, and alleviate hangover symptoms such as dizziness and agitation; it can also significantly reduce serum AST and ALT transaminase levels, inhibit hepatocyte inflammatory infiltration and fatty degeneration, and repair alcohol-induced liver tissue damage. Its liver protection and repair effects are superior to conventional positive control drugs. It achieves an integrated effect of "hangover relief + liver protection + repair". Attached Figure Description

[0020] Figure 1 This is a graph showing the detection results of ADH activity in the serum of intoxicated mice in Test Example 1; Figure 2 This is a graph showing the detection results of ALDH activity in the serum of intoxicated mice in Test Example 1; Figure 3 This is a graph showing the detection results of AST activity in the serum of intoxicated mice in Test Example 2; Figure 4 This is a graph showing the detection results of ALT activity in the serum of intoxicated mice in Test Example 2; Figure 5 This is an HE-stained section of liver tissue from an intoxicated mouse in test example 2. Detailed Implementation

[0021] The following provides a detailed description of specific embodiments of the present invention. It should be understood that the specific embodiments described herein are for illustrative and explanatory purposes only and are not intended to limit the scope of the invention.

[0022] This invention utilizes network pharmacology to combine 106 kinds of Chinese medicinal herbs with medicinal and edible properties, and uses advanced methods such as uniform design and pharmacological evaluation to screen and obtain the medicinal and edible compound composition for relieving hangovers and protecting the liver.

[0023] The medicinal and edible compound composition for relieving hangovers and protecting the liver described in this invention is made from jujube seed, mulberry leaf, jujube, eucommia leaf and poria cocos. The amount of jujube seed is 6-12 parts by weight, the amount of mulberry leaf is 5-8 parts by weight, the amount of jujube is 2-9 parts by weight, the amount of eucommia leaf is 2-10 parts by weight and the amount of poria cocos is 2-12 parts by weight.

[0024] In this invention, five medicinal materials—sour jujube seed, mulberry leaf, jujube, eucommia leaf, and poria cocos—are scientifically combined and synergistically enhanced, with each component complementing the other's effects and precisely targeting the pathways of alcohol metabolism and liver damage repair.

[0025] Poria cocos: Its core function is to protect and nourish the liver, which can significantly improve the pathological state of alcoholic liver disease, regulate the liver inflammatory pathway, and inhibit hepatocyte damage. Mulberry leaves: rich in mulberry leaf alkaloids, which can effectively improve alcohol-induced liver enlargement, protect the normal tissue structure of the liver, and reduce fatty infiltration of hepatocytes; Jujube and Eucommia ulmoides leaves: rich in natural polysaccharides, possessing excellent antioxidant activity, which can eliminate oxygen free radicals produced by alcohol metabolism and reduce oxidative damage to liver cells; Sour jujube seed: It has both calming and soothing effects, which can relieve symptoms such as central nervous system hyperactivity, dizziness and irritability after drinking, improve the discomfort of drunkenness, and help improve the comfort after drinking.

[0026] In this invention, by combining five medicinal herbs—sour jujube seed, mulberry leaf, jujube, eucommia leaf, and poria cocos—in a specific ratio, the components can work synergistically. On the one hand, they can enhance the activity of alcohol dehydrogenase and aldehyde dehydrogenase, accelerate the metabolism and decomposition of ethanol and aldehyde, and quickly relieve drunkenness. On the other hand, they can have antioxidant, anti-inflammatory, and liver cell repair effects, preventing and improving alcoholic liver damage from the source, thereby achieving the dual effects of relieving drunkenness and protecting the liver.

[0027] In this invention, the dosage form of the medicinal and edible compound composition for relieving hangovers and protecting the liver is not particularly limited and can be any dosage form commonly used in the art. In some specific embodiments, the dosage form of the medicinal and edible compound composition for relieving hangovers and protecting the liver is powder, granules, tablets, oral liquid, or capsules.

[0028] The preparation method of the medicinal and edible homologous compound composition for relieving hangovers and protecting the liver according to the present invention includes the following steps: (1) Soak the jujube seed, mulberry leaf, jujube, eucommia leaf and poria in water, decoct once, and then filter to obtain a filtrate and residue; (2) Boil the filter residue in water a second time, then filter it, collect the second filtrate, and combine the first filtrate and the second filtrate; The amount of jujube seed is 6-12 parts by weight, the amount of mulberry leaf is 5-8 parts by weight, the amount of jujube is 2-9 parts by weight, the amount of eucommia leaf is 2-10 parts by weight, and the amount of poria is 2-12 parts by weight.

[0029] In the method described in this invention, in step (1), during the soaking and the first decoction process, the amount of water can be 10-15 times the total weight of the jujube seed, the mulberry leaf, the jujube, the eucommia leaf and the poria cocos. Specifically, for example, it can be 10 times, 11 times, 12 times, 13 times, 14 times, 15 times, or any value between any two of the above values.

[0030] In the method described in this invention, in step (1), the soaking time can be 30-45 min.

[0031] In the method described in this invention, the conditions for the first decoction in step (1) may include: a temperature of 95-100℃ at a gentle boil and a time of 1.5-2h.

[0032] In the method described in this invention, in step (2), the amount of water used in the secondary decoction process can be 8-9 times the mass of the filter residue obtained in step (1).

[0033] In the method described in this invention, the conditions for the second decoction in step (2) may include: a temperature of 95-100℃ at a gentle boil and a time of 1-1.5h.

[0034] In some embodiments, in order to prepare powder or granules, the preparation method of the medicinal and edible compound composition for relieving hangovers and protecting the liver further includes: concentrating the combined filtrate by rotary evaporation under reduced pressure to obtain an extract, and then subjecting the extract to ultra-low temperature freezing and vacuum freeze-drying in sequence.

[0035] In the above embodiments, the conditions for vacuum rotary evaporation concentration may include: a temperature of 50-60°C, a vacuum negative pressure of 0.02-0.04 MPa, and an absolute pressure of 60-80 kPa.

[0036] In the above embodiments, the conditions for ultra-low temperature freezing may include: a temperature of -100°C to -50°C and a time of 24-36 hours.

[0037] The medicinal and edible compound composition for relieving hangovers and protecting the liver described in this invention can be used to prepare functional foods or health products for preventing acute alcohol poisoning, relieving hangover symptoms, and repairing alcoholic liver damage.

[0038] To make the objectives and advantages of the present invention clearer, the technical solutions and effects of the present invention will be further described in detail below with reference to specific embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and should not be used to limit the present invention.

[0039] Unless otherwise specified, the following embodiments do not include components other than unavoidable impurities. Unless otherwise specified, the drugs and instruments used in the examples are conventional choices in the art. Experimental methods in the examples that do not specify specific conditions were performed under conventional conditions, such as those described in literature, books, or methods recommended by the manufacturer.

[0040] Example 1 In this embodiment, the total amount of Poria cocos, mulberry leaf, jujube, Eucommia ulmoides leaf, and jujube seed is 35g. Specifically, the amount of jujube seed is 6.85g, mulberry leaf is 5.33g, jujube is 2.28g, Eucommia ulmoides leaf is 9.13g, and Poria cocos is 11.41g.

[0041] The specific preparation steps are as follows: (1) Select high-quality Poria cocos, jujube, jujube seed, mulberry leaf and Eucommia leaf that are free from mold and insects, wash them with clean water to remove surface impurities, and air dry them at room temperature for later use. (2) Weigh the medicinal materials, add 10 times the weight of water, soak at room temperature for 30 minutes, decoct at 100℃ for 1.5 hours, and then filter to obtain the first filtrate and the residue. (3) Add 8 times the weight of water to the filter residue, continue to boil at 100°C for 1 hour, filter and collect the secondary filtrate; (4) Combine the two filtrates and place them in a rotary evaporator. Concentrate the filtrate under reduced pressure at 60°C, a vacuum negative pressure of 0.04 MPa, and an absolute pressure of 80 kPa to obtain an extract. Place the extract in a -80°C freezer for freezing and shaping, and freeze dry under vacuum for 30 h to obtain a dry powder. (5) Pass the freeze-dried powder through an 80-mesh sieve, mix thoroughly, seal and store in the dark to obtain 5.62g of the composition.

[0042] Example 2 The pharmaceutical composition was prepared according to the method of Example 1, except that the specific amounts of the five medicinal materials were as follows: 8.26g of jujube seed, 7.87g of mulberry leaf, 7.08g of jujube, 2.36g of eucommia leaf, and 9.44g of poria cocos, resulting in a final composition of 6.89g.

[0043] Example 3 The pharmaceutical composition was prepared according to the method of Example 1, except that the specific amounts of the five medicinal materials were as follows: 11.54g of jujube seed, 6.15g of mulberry leaf, 8.08g of jujube, 6.92g of eucommia leaf, and 2.31g of poria cocos, resulting in a final composition of 9.69g.

[0044] Comparative Example 1 The pharmaceutical composition was prepared according to the method of Example 1, except that jujube seed was not added. The specific dosage of the medicinal materials was as follows: 5.33g of mulberry leaf, 2.28g of jujube, 9.13g of eucommia leaf, and 11.41g of poria cocos, with a total raw material weight of 28.15g, resulting in a final composition of 5.01g.

[0045] Comparative Example 2 The pharmaceutical composition was prepared according to the method of Example 1, except that mulberry leaves were not added. The specific amounts of medicinal materials used were: 6.85g of jujube seed, 2.28g of jujube, 9.13g of eucommia leaf, and 11.41g of poria cocos, with a total raw material amount of 29.67g, resulting in a final composition of 4.72g.

[0046] Comparative Example 3 The pharmaceutical composition was prepared according to the method of Example 1, except that jujubes were not added. The specific amounts of medicinal materials used were: 6.85g of jujube seed, 5.33g of mulberry leaf, 9.13g of eucommia leaf, and 11.41g of poria cocos, for a total raw material weight of 32.72g, resulting in a final composition weight of 4.80g.

[0047] Comparative Example 4 The pharmaceutical composition was prepared according to the method of Example 1, except that Eucommia ulmoides leaves were not added. The specific amounts of medicinal materials used were: 6.85g of Ziziphus jujuba seed, 5.33g of mulberry leaf, 2.28g of jujube, and 11.41g of Poria cocos, for a total raw material weight of 25.87g, resulting in a final composition weight of 3.89g.

[0048] Comparative Example 5 The pharmaceutical composition was prepared according to the method of Example 1, except that Poria cocos was not added. The specific amounts of medicinal materials used were: 6.85g of Ziziphus jujuba seed, 5.33g of mulberry leaf, 2.28g of jujube, and 9.13g of Eucommia ulmoides leaf, with a total raw material weight of 23.59g, resulting in a final composition weight of 4.03g.

[0049] Test case laboratory animals SPF - level 7 - week - old male Balb / c mice were used in this project. They were purchased from Hubei Experimental Animal Research Center, and the license batch number for use by the experimental unit is SYXK-(E)2023 - 0067. This experimental protocol has been approved by the Animal Ethics Committee of the experimental unit, with the ethics number: HUCMS00311966. All experimental animals were housed in an environment with a temperature of 22 - 26°C, a relative humidity of 40 - 70%, and a 12 - hour light / 12 - hour dark cycle. The animals had free access to food and water.

[0050] Experimental drugs Based on the human clinical daily dose of 35 g / 70 kg of crude drug, and converted according to the body surface area, the equivalent dose for mice is 4.55 g of crude drug / kg body weight. The compositions prepared in Examples 1 - 3 and Comparative Examples 1 - 5 were weighed respectively, and then dissolved and made up to volume with 0.5% sodium carboxymethylcellulose solution to prepare the medicinal liquids. The final concentrations of the medicinal liquids prepared in Examples 1 - 3 and Comparative Examples 1 - 5 were all 0.455 g of crude drug / mL in terms of the amount of crude drug.

[0051] Test Example 1 To explore the effects of different compatibility schemes on the hangover effect, this study evaluated the compositions of Examples 1 - 3 and Comparative Examples 1 - 5. By recording the hangover time of the drunken model mice and detecting the activity indexes of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) in the mice, the intervention effects of each group were comprehensively compared.

[0052] 1. Experimental method: The 66 purchased Balb / c mice were adaptively raised for one week. Before the experiment, they were randomly divided into 11 groups, labeled as the blank group (B), the model group (M), the positive control group (PC), and the dosing groups (G1 - G3, C1 - C5), with 6 mice in each group. The blank group was intragastrically administered 0.013 mL·g -1 of normal saline, the model group was intragastrically administered 0.013 mL·g -1 of 52° Hongxing Erguotou to obtain the drunken model, the positive control group was intragastrically administered silymarin 0.1 g·kg -1 , the dosing groups G1 - G3 were intragastrically administered the medicinal liquids prepared in Examples 1 - 3 respectively, and the dosing groups C1 - C5 were intragastrically administered the medicinal liquids prepared in Comparative Examples 1 - 5 respectively. The intragastric administration volume of each dosing group was 10 mL / kg. After 30 minutes, 0.013 mL·g -1 of 52° Hongxing Erguotou was intragastrically administered.

[0053] The time from alcohol administration to the disappearance of the righting reflex in the mice was recorded as the drunken latency period, and the time from the disappearance of the righting reflex to recovery was recorded as the drunken time.

[0054] 2. Judgment Criteria: The disappearance of the righting reflex and grasping behavior in mice after gavage was used as the indicators of intoxication. The mice's movement was observed every 3 minutes after gavage. The mice were placed face down and abdomen up in the palm of the hand; if they failed to turn over within 30 seconds, they were considered intoxicated. The mice were placed on a wire mesh and observed for grasping; if they did not fall within 10 seconds, they were considered not intoxicated. The time from gavage administration to the disappearance of the righting reflex was the latency period of intoxication, and the time from the disappearance of the righting reflex to its recovery was the intoxication time. The sobering time was the sum of the latency period and the intoxication time; specific data are shown in Table 2.

[0055] Data were statistically processed using GraphPad Prism 10 software. *P<0.05 indicates statistical difference, and **P<0.01 indicates significant difference.

[0056] Table 2

[0057] As shown in Table 2, the time to sobering up was significantly shortened in intoxicated mice after drug treatment. Furthermore, the statistically significant differences in sobering effects were observed in Examples 1-3, Comparative Examples 2 and 3, and the positive control group.

[0058] 3. ALDH and ADH activity detection Detection method: Mice were fully awakened 4 hours after intoxication. Blood was collected, and the mice were euthanized by cervical dislocation. The livers were removed and frozen at -80℃. Blood samples were allowed to stand for at least one hour, and then tested at 4℃ and 3000 rpm. -1 Centrifuge for 15 min under the specified conditions, collect the supernatant and freeze at -80℃ for later use. Serum samples were obtained and tested using kits, specifically the alcohol dehydrogenase (ADH) test kit and the aldehyde dehydrogenase (ALDH) test kit.

[0059] Data were statistically processed using GraphPad Prism 10 software. *P<0.05 indicates a statistically significant difference, **P<0.01 indicates a significant difference, ***P<0.001 indicates an extremely significant difference, and ****P<0.0001 indicates a highly significant difference.

[0060] Detection results: Serum ADH and ALDH activities in each group of intoxicated mice were as follows: Figure 1 and Figure 2 As shown. Examples 1-3 (G1-G3) all enhanced the activity of ADH and ALDH in mice, and the differences among the groups were quite significant. There were no statistically significant differences in ADH levels in Comparative Examples 1-3 and Comparative Example 5, while there was a statistically significant difference in ADH levels in Comparative Example 4, but no statistically significant difference in ALDH levels in Comparative Example 4.

[0061] Based on the combined results of ALDH index, ADH index, and alcohol detoxification time in mice, it can be seen that Examples 1-3 can significantly improve the activity of liver enzymes, accelerate metabolism, thereby producing an alcohol detoxification effect and shortening the alcohol detoxification time, while the combined effect of Comparative Examples 1-5 is minimal.

[0062] Test Example 2 This test case further evaluates the effect of the composition on improving liver injury. When hepatocytes are damaged or die, the levels of two transaminases, AST and ALT, will rise abnormally in the blood; therefore, the levels of these two transaminases are important indicators for determining liver injury.

[0063] 1. Experimental Methods: Sixty-six purchased Balb / c mice were acclimatized for one week and then randomly divided into 11 groups before the experiment, labeled as blank group (B), model group (M), positive control group (PC), and drug treatment groups (G1-G3, C1-C5), with 6 mice in each group. The blank group was administered 0.013 mL / g of the drug via gavage. -1 The model group was given 0.013 mL / g of physiological saline by gavage. -1 A model of intoxication was obtained by administering 52° Red Star Erguotou (a type of Chinese liquor). The positive control group was given silymarin (0.1 g·kg⁻¹) by gavage. -1 Groups G1-G3 were administered the drug solutions prepared in Examples 1-3 by gavage, while groups C1-C5 were administered the drug solutions prepared in Comparative Examples 1-5 by gavage. The gavage volume for each group was 10 mL / kg. After 30 min, 0.013 mL / g was administered by gavage. -1 The mice were treated with 52° Red Star Erguotou liquor. Four hours later, blood was collected from the mice, the mixture was allowed to stand, centrifuged, the supernatant was collected, and the supernatant was stored at -80°C. The mice were euthanized by cervical dislocation, and the livers were dissected, weighed, and the liver coefficient was calculated. Appropriately sized tissue samples were taken and preserved at 4°C with tissue fixative. The remaining liver tissue was stored at -80°C. Liver tissue sections from each group were stained with hematoxylin and eosin (HE), and the degree of inflammation and fat accumulation in the liver tissue was assessed through pathological observation.

[0064] Data were statistically processed using GraphPad Prism 10 software. *P<0.05 indicates a statistically significant difference, **P<0.01 indicates a significant difference, ***P<0.001 indicates an extremely significant difference, and ****P<0.0001 indicates a highly significant difference.

[0065] 2. Test Results: The detection results of AST and ALT activities in the serum of intoxicated mice are as follows: Figure 3 and Figure 4 As shown, compared with the model group, the levels of AST and ALT in mice in Examples 1-3 and the positive control group were significantly reduced, proving that Examples 1-3 can significantly reduce liver damage indicators and exert a liver-protective effect. Comparative examples 2 and 4 in the comparative examples showed significant differences and had a more significant liver-protective effect.

[0066] HE staining results of liver tissue pathology sections from intoxicated mice are as follows: Figure 5 The results showed that in the control group, the hepatocytes of mice were neatly arranged, clearly structured, without edema or fatty degeneration, with a small number of dead hepatocytes and no inflammatory cell infiltration. The model group showed typical pathological features, with unevenly sized hepatocytes, numerous small vesicular fat vacuoles, and inflammatory cell infiltration. The liver tissue of mice in Examples 1-3 and the positive drug group showed no obvious abnormalities, while the liver tissue of mice in Comparative Examples 1-5 showed a few fatty infiltrations, inflammatory infiltrations, and tissue necrosis.

[0067] Based on the test results of the above embodiments and comparative examples, it can be seen that the medicinal and edible compound composition for relieving hangovers and protecting the liver described in this invention has the effects of relieving hangovers and protecting the liver.

[0068] The preferred embodiments of the present invention have been described in detail above; however, the present invention is not limited thereto. Within the scope of the inventive concept, various simple modifications can be made to the technical solutions of the present invention, including combinations of various technical features in any other suitable manner. These simple modifications and combinations should also be considered as the content disclosed in the present invention and are all within the protection scope of the present invention.

Claims

1. A compound composition for relieving hangovers and protecting the liver, characterized in that, This medicinal and edible compound formula for relieving hangovers and protecting the liver is made from jujube seed, mulberry leaf, jujube, eucommia leaf, and poria cocos. The amount of jujube seed is 6-12 parts by weight, the amount of mulberry leaf is 5-8 parts by weight, the amount of jujube is 2-9 parts by weight, the amount of eucommia leaf is 2-10 parts by weight, and the amount of poria is 2-12 parts by weight.

2. The medicinal and edible compound composition for relieving hangovers and protecting the liver according to claim 1, characterized in that, The dosage form of the medicinal and edible compound for relieving hangovers and protecting the liver is powder, granules, tablets, oral liquid or capsules.

3. A method for preparing a compound composition for relieving hangovers and protecting the liver, characterized in that, The method includes the following steps: (1) Soak the jujube seed, mulberry leaf, jujube, eucommia leaf and poria in water, decoct once, and then filter to obtain a filtrate and residue; (2) Boil the filter residue in water a second time, then filter it, collect the second filtrate, and combine the first filtrate and the second filtrate; The amount of jujube seed is 6-12 parts by weight, the amount of mulberry leaf is 5-8 parts by weight, the amount of jujube is 2-9 parts by weight, the amount of eucommia leaf is 2-10 parts by weight, and the amount of poria is 2-12 parts by weight.

4. The method according to claim 3, characterized in that, In step (1), during the soaking and the first decoction process, the amount of water used is 10-15 times the total weight of the jujube seed, the mulberry leaf, the jujube, the eucommia leaf and the poria.

5. The method according to claim 3 or 4, characterized in that, In step (1), the soaking time is 30-45 minutes.

6. The method according to claim 3 or 4, characterized in that, In step (1), the conditions for the first decoction include: a temperature of 95-100℃ at a gentle boil and a time of 1.5-2 hours.

7. The method according to claim 3, characterized in that, In step (2), the amount of water used in the secondary decoction process is 8-9 times the mass of the filter residue obtained in step (1).

8. The method according to claim 3 or 7, characterized in that, In step (2), the conditions for the second decoction include: a temperature of 95-100℃ at a gentle boil and a time of 1-1.5h.

9. The method according to any one of claims 3-8, characterized in that, The method further includes: concentrating the combined filtrate by rotary evaporation under reduced pressure to obtain an extract, and then subjecting the extract to ultra-low temperature freezing and vacuum freeze-drying in sequence; Preferably, the conditions for vacuum rotary evaporation concentration include: a temperature of 50-60℃, a vacuum negative pressure of 0.02-0.04MPa, and an absolute pressure of 60-80kPa; Preferably, the conditions for ultra-low temperature freezing include: a temperature of -100°C to -50°C and a time of 24-36 hours.

10. The use of the medicinal and edible compound composition for relieving hangovers and protecting the liver as described in claim 1 or 2, or the medicinal and edible compound composition for relieving hangovers and protecting the liver prepared by the method described in any one of claims 3-9, in the preparation of functional foods or health products for preventing acute alcohol poisoning, relieving hangover symptoms, and repairing alcoholic liver damage.

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