A composition for strengthening the body resistance and improving the body immunity and a preparation method thereof

Abstract

The present application relates to the technical field of traditional Chinese medicine preparation, and particularly relates to a composition for strengthening body resistance and improving immune function of the body and a preparation method thereof. The composition comprises Cistanche, Rhizoma Polygonati, Astragalus and Citrus medica. The composition provided by the present application can increase the spleen index, thymus index and the number of peripheral blood leukocytes, regulate the expression of immunoglobulin and cytokine, increase the number of bone marrow nucleated cells and the mass concentration of DNA, protect the bone marrow hematopoietic function, and improve the immune function of the body. The composition can regulate the qi and blood of viscera and stabilize the body resistance, and is suitable for long-term conditioning of people with deficiency of qi and blood and low immune function.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine preparation technology, and in particular to a composition for treating immunodeficiency, its preparation method and application. Background Technology

[0002] The immune system is the body's protective mechanism. Its core function is to identify and eliminate pathogenic microorganisms and diseased cells that invade the body. It relies on the regulatory role of the immune system to stabilize the internal physiological environment and ensure normal bodily functions. A weakened immune system indicates a decline in the immune response, resulting in insufficient ability to intercept harmful microorganisms such as bacteria and viruses, and difficulty in promptly eliminating diseased cells. This is a manifestation of functional imbalance, a sub-healthy or pathological condition. Typical characteristics include frequent illnesses, slow recovery from infections, and often accompanied by general discomfort such as physical weakness, fatigue, weakness in the limbs, and poor appetite. If the immune system is chronically weak, it not only easily induces various physical illnesses but also increases the probability of developing mental problems such as mood disorders and depression. Prolonged, untreated cases can even increase the likelihood of developing malignant diseases.

[0003] While Traditional Chinese Medicine (TCM) lacks a specific terminology directly equivalent to modern medical terms for immunity, the physical manifestations of weakened immunity—such as susceptibility to illness, physical weakness, and poor resistance to pathogens—are all categorized under the syndrome of "deficiency of vital energy" within the TCM diagnostic system. Chinese medicinal herbs contain various active components that can regulate immune responses through multiple targets and pathways, and long-term use is less likely to lead to tolerance. TCM's core treatment principle of supporting vital energy and eliminating pathogenic factors offers unique and irreplaceable advantages in repairing damaged immune function, preventing recurrent infections, and in comprehensive cancer treatment. Summary of the Invention

[0004] To address the aforementioned problems, this invention provides a composition and its preparation method for strengthening the body's resistance and improving immune function. The composition provided by this invention can increase spleen and thymus indices and peripheral blood leukocyte counts, regulate immunoglobulin and cytokine expression, simultaneously increase the number of nucleated cells and DNA concentration in bone marrow, protect bone marrow hematopoietic function, and regulate the body's immune function. This composition can harmonize the Qi and blood of the internal organs... It strengthens the body's vital energy and is suitable for long-term conditioning for people with weak constitution, low energy, and low immune function.

[0005] To achieve the above objectives, the present invention provides the following technical solution: This invention provides a composition for strengthening the body's resistance and improving its immune function, comprising Cistanche deserticola, Polygonatum sibiricum, Astragalus membranaceus, and Citrus medica.

[0006] Preferably, the mass ratio of Cistanche deserticola, Polygonatum sibiricum, Astragalus membranaceus, and Citrus medica is 5:10:10:5.

[0007] The present invention also provides a method for preparing the composition described in the above technical solution, comprising the following steps: 1) Soak the Cistanche deserticola, Polygonatum sibiricum, Astragalus membranaceus, and Citrus medica in water to obtain a soaking solution; 2) Boil the soaking material obtained in step 1), and dry the resulting decoction to obtain the composition.

[0008] Preferably, the total mass ratio of Cistanche deserticola, Polygonatum sibiricum, Astragalus membranaceus, and Citrus medica to water in step 1) is 1:10.

[0009] Preferably, the soaking time in step 1) is 2 hours.

[0010] Preferably, the conditions for decocting in step 2) include: decocting twice, for 1 hour each time.

[0011] Preferably, the drying conditions in step 2) include: combining the decoctions obtained from two decoctions and filtering them, concentrating the filtrate to obtain an extract, and drying the extract at 40°C to a constant weight to obtain the composition.

[0012] The present invention also provides the application of the composition described in the above technical solution in the preparation of products that improve the body's immune function.

[0013] Cistanche deserticola tonifies kidney yang and nourishes essence and blood, warming without being drying, thus strengthening the immune foundation from the root of innate constitution. Traditional Chinese medicine believes the kidney is the root of innate constitution; abundant kidney essence generates vital energy, and sufficient vital energy steadily enhances the body's ability to resist external pathogens. Polygonatum sibiricum tonifies qi and nourishes yin, strengthens the spleen and moistens the lungs, replenishing the source of qi and blood from the root of acquired constitution. Traditional Chinese medicine believes the spleen is the root of acquired constitution; a healthy spleen and stomach ensure the source of qi and blood production, and abundant qi and blood strengthen the defensive qi, creating a robust body surface barrier that effectively resists external pathogens. The two herbs work synergistically to tonify the body both before and after birth, strengthening the foundation and improving the root causes of deficiency and weak immunity. Astragalus membranaceus tonifies qi and strengthens the exterior, boosts yang, supports the body's vital energy, and strengthens the body's surface defense. Traditional Chinese medicine considers Astragalus membranaceus (Huangqi) to be the most potent of all qi-tonifying herbs. It can replenish the qi of the spleen and lungs, nourish the body's defensive qi, and enhance the body's ability to resist disease. It assists Cistanche deserticola (Rou Cong Rong) and Polygonatum sibiricum (Huang Jing) in strengthening the body's vital energy and replenishing qi and blood, further boosting immunity and improving symptoms such as qi deficiency, fatigue, and susceptibility to illness. Citrus medica (Bao Shou) soothes the liver, regulates qi, harmonizes the stomach, and relieves bloating. Its mild properties specifically address the drawbacks of excessive tonification. Cistanche deserticola and Polygonatum sibiricum are rich and cloying; long-term use alone can easily obstruct the spleen and stomach, hindering qi flow and causing abdominal distension, poor appetite, and digestive problems. Citrus medica, however, promotes qi circulation without depleting it and soothes the liver without harming yin. It effectively relieves spleen and stomach stagnation, harmonizes liver and spleen qi, and counteracts the drawbacks of tonifying herbs that are too rich and cloying. Simultaneously, it unblocks the body's qi pathways, promoting the smooth distribution of tonified qi and blood throughout the body, resolving the problem of inability to tolerate tonification or stagnation caused by tonification. The combination of various herbs nourishes the innate constitution, replenishes the acquired constitution, strengthens the body's defenses, and promotes the flow of Qi. It replenishes without causing stagnation, nourishes without being greasy, supports the body's vital energy without obstructing pathogens, and unblocks the flow of Qi without harming the body's vital energy. It comprehensively harmonizes the Qi and blood of the internal organs and stabilizes the body's vital energy, making it suitable for long-term conditioning for people with weak constitution, low Qi, and low immune function.

[0014] Beneficial effects: The composition provided by this invention can increase the spleen and thymus indices and the number of peripheral blood leukocytes, regulate the expression of immunoglobulins and cytokines, increase the number of nucleated cells in the bone marrow and the concentration of DNA, protect bone marrow hematopoietic function, and improve the body's immune function. This composition can harmonize the qi and blood of the internal organs and stabilize the body's vital energy, making it suitable for long-term conditioning in people with weak constitution, low qi, and low immune function. Attached Figure Description

[0015] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the embodiments will be briefly described below.

[0016] Figure 1 The values ​​were: spleen index and thymus index in mice; #P<0.05, ##P<0.01, ###P<0.001 compared with the control group; ×P<0.05, ××P<0.01, ×××P<0.001 compared with the model group; Figure 2The values ​​represent the mass concentrations of immunoglobulin proteins and cytokines in the peripheral blood of mice; #P<0.05, ##P<0.01, ###P<0.001 compared with the control group; ×P<0.05, ××P<0.01, ×××P<0.001 compared with the model group; Figure 3 Peripheral blood cell count in mice; #P<0.05, ##P<0.01, ###P<0.001, compared with the control group; ×P<0.05, ××P<0.01, ×××P<0.001, compared with the model group; Figure 4 The values ​​were: nucleated cell count and DNA concentration in mouse bone marrow; #P<0.05, ##P<0.01, ###P<0.001 compared with the control group; ×P<0.05, ××P<0.01, ×××P<0.001 compared with the model group. Detailed Implementation

[0017] This invention provides a composition for strengthening the body's resistance and improving its immune function, comprising Cistanche deserticola, Polygonatum sibiricum, Astragalus membranaceus, and Citrus medica. In this invention, the preferred mass ratio of Cistanche deserticola, Polygonatum sibiricum, Astragalus membranaceus, and Citrus medica is 5:10:10:5.

[0018] The present invention also provides a method for preparing the composition described in the above technical solution, comprising the following steps: 1) Soak the Cistanche deserticola, Polygonatum sibiricum, Astragalus membranaceus, and Citrus medica in water to obtain a soaking solution; 2) Boil the soaking material obtained in step 1), and dry the resulting decoction to obtain the composition.

[0019] This invention involves soaking a mixture of Cistanche deserticola, Polygonatum sibiricum, Astragalus membranaceus, and Citrus medica in water to obtain a soaking solution. In this invention, the preferred mass ratio of the total mass of Cistanche deserticola, Polygonatum sibiricum, Astragalus membranaceus, and Citrus medica to water is 1:10. The preferred soaking time is 2 hours.

[0020] This invention involves decocting the obtained soaking material, drying the resulting decoction, and obtaining a composition. Preferably, the decoction conditions include: decocting twice, 1 hour each time. Preferably, the drying conditions include: combining the decoctions obtained from the two decoctions, filtering, concentrating the filtrate to obtain an extract, and drying the extract at 40°C to constant weight to obtain the composition.

[0021] The present invention also provides the application of the composition described in the above technical solution in the preparation of products that improve the body's immune function.

[0022] To further illustrate the present invention, the following detailed description is provided in conjunction with embodiments, but these should not be construed as limiting the scope of protection of the present invention.

[0023] Example 1 A composition for strengthening the body's resistance and improving its immune function comprises the following steps: 1) Mix 5g of Cistanche deserticola, 10g of Polygonatum sibiricum, 10g of Astragalus membranaceus, and 5g of Citrus medica with water at a mass ratio of 1:10 and soak for 2 hours to obtain the soaking material; 2) The soaking material obtained in step 1) is decocted, and the resulting decoction is dried to obtain the composition; wherein, the decoction is decocted twice, each time for 1 hour; the decoctions obtained from the two decoctions are combined and filtered, and the filtrate is concentrated by a rotary evaporator to obtain an extract. The extract is placed in a 40°C oven and dried to constant weight to obtain the composition.

[0024] Example of drug efficacy Preparation of experimental drugs: The powdered composition prepared in Example 1 was dissolved in purified water to form a solution with a mass concentration of 200 mg / mL. Cyclophosphamide (CTX) was dissolved in physiological saline to form a solution with a mass concentration of 12.5 mg / mL. Thirty healthy SPF-grade BALB / c male mice, weighing 25 g and aged 7-8 weeks, were randomly divided into three groups of 10 mice each after 3 days of acclimatization feeding.

[0025] Model group: Intraperitoneal injection of CTX (50 mg / kg) for 4 consecutive days; Control group: Intraperitoneal injection of the same volume of normal saline as the model group, for 4 consecutive days; Composition group: intraperitoneal injection of CTX (50 mg / kg), followed by gavage administration of composition solution (0.8 g / kg) at 4-hour intervals for 4 consecutive days; Starting on day 5, the combination group continued to be administered the combination solution by gavage, while the control and model groups were administered the same amount of purified water by gavage until the end of the experiment on day 28. After the last administration, the mice were fasted overnight for 12 hours, and their body weight was measured the next morning. After anesthesia, the eyeballs were enucleated to collect blood, and the mice were euthanized by cervical dislocation. The spleen, thymus, and other biological materials were dissected and collected for index testing.

[0026] Indicator Testing: (1) Spleen index and thymus index measurement Mice were weighed and euthanized by dislocation of the neck. The spleen and thymus were quickly dissected and separated. Surface adipose tissue was removed and blood was dried. The organs were weighed. The spleen and thymus indices for each group were calculated using the formula: Organ Index = Organ Wet Weight (mg) / Animal Body Weight (g). The data were recorded and statistically analyzed.

[0027] (2) Measurement of peripheral blood immunoglobulins and cytokines After the experiment, whole blood was collected from mice and centrifuged at 3000 r / min for 15 min, and serum was collected. The concentrations of IgG, IgM, IFN-γ, and IL-10 in the serum were determined according to the ELISA kit instructions. Reagents were pre-balanced, serum samples and standards were diluted, and samples were added to coated plates and incubated for washing. Enzyme-labeled secondary antibody was added and incubated in the dark, unbound material was washed again, and chromogenic buffer was added for color development in the dark. The reaction was terminated with stop solution. The OD values ​​at the corresponding wavelengths were measured using an ELISA reader, and the concentrations of peripheral blood immunoglobulins and cytokines were calculated based on the standard curve.

[0028] (3) Peripheral blood leukocyte count Two drops of blood were collected from the mouse eyeballs and immediately transferred to an anticoagulant centrifuge tube. The tubes were thoroughly mixed by inverting and then placed in a blood analyzer for testing. White blood cell counts for each group were recorded, and the data were compiled for statistical analysis.

[0029] (4) Bone marrow nucleated cell count and DNA mass concentration determination After euthanizing the animal, the femur was dissected, and the bone marrow cavity was flushed to obtain a bone marrow cell suspension. Bone debris was removed by filtration, and the suspension was thoroughly mixed by pipetting. A small amount of the suspension was diluted and added to a hemocytometer for cell counting. An equal volume of bone marrow cells was taken, and the cells were lysed and DNA purified using a DNA extraction kit. The DNA solution was then eluted. The absorbance at 260 nm was measured using a microplate reader to calculate the DNA concentration.

[0030] Results and Analysis: (1) Effects of the composition on immune organs of immunocompromised mice Figure 1 Data showed that, compared with the control group, the spleen index and thymus index of the model group decreased significantly, indicating that CTX caused serious damage to the immune organs of mice and the mouse model of low immunity was successfully established. After intervention with the composition, the spleen index and thymus index of mice increased significantly, indicating that the composition has a certain alleviating effect on mice with low immunity and improves the body's immune function to a certain extent.

[0031] (2) Effects of the composition on peripheral blood immunoglobulins and cytokines in immunocompromised mice Figure 2 Data showed that the serum IgG and IgM levels in the model group mice were significantly lower than those in the control group, and the levels of IgG and IgM significantly increased after intervention with the composition. The peripheral blood IFN-γ concentration in the model group mice was significantly lower than that in the control group, while the IFN-γ concentration in the composition group was significantly higher than that in the model group. Conversely, the peripheral blood IL-10 concentration in the model group mice was significantly higher than that in the control group, while the IL-10 concentration in the composition group was significantly lower than that in the model group. These results indicate that the composition has a significant effect on improving immunoglobulins and cytokines in immunocompromised mice.

[0032] (3) Effect of the composition on the number of peripheral blood leukocytes in immunocompromised mice Figure 3 The results showed that, compared with the normal control group, the number of peripheral blood leukocytes in the model group mice was significantly decreased; after intervention with the composition, the level of peripheral blood leukocytes in the mice significantly increased. These results suggest that the composition can increase the number of peripheral blood leukocytes in immunocompromised model mice, thereby improving the body's immune function.

[0033] (4) Effects of the composition on the number of nucleated cells and DNA concentration in the bone marrow of immunocompromised mice CTX is an alkylating agent that directly alkylates DNA, blocks DNA replication, arrests the proliferation of bone marrow hematopoietic stem cells, and significantly reduces the total number of nucleated cells in the bone marrow. This decrease in cell count leads to a decrease in total DNA content in the bone marrow sample. Our experimental results showed that the number of nucleated cells and DNA concentration in the bone marrow of the model group mice were significantly lower than those in the control group. After intervention with the combined formula, both the number of nucleated cells and DNA concentration in the bone marrow significantly increased. (See attached figures). Figure 4 The composition suggests that it can protect bone marrow and restore hematopoietic and immune functions.

[0034] In summary, the composition can increase the spleen and thymus index and the number of peripheral blood leukocytes, regulate the expression of immunoglobulins and cytokines, increase the number of nucleated cells in the bone marrow and the concentration of DNA, protect bone marrow hematopoietic function, and improve the body's immune function.

[0035] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, and not all embodiments. People can obtain other embodiments based on these embodiments without creative effort, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A composition for strengthening the body's resistance and improving its immune function, characterized in that, It includes Cistanche deserticola, Polygonatum sibiricum, Astragalus membranaceus, and Citrus medica.

2. The composition according to claim 1, characterized in that, The mass ratio of Cistanche deserticola, Polygonatum sibiricum, Astragalus membranaceus, and Citrus medica is 5:10:10:

5.

3. A method for preparing the composition according to claim 1 or 2, characterized in that, Includes the following steps: 1) Soak the Cistanche deserticola, Polygonatum sibiricum, Astragalus membranaceus, and Citrus medica in water to obtain a soaking solution; 2) Boil the soaking material obtained in step 1), and dry the resulting decoction to obtain the composition.

4. The preparation method according to claim 3, characterized in that, In step 1), the total mass ratio of Cistanche deserticola, Polygonatum sibiricum, Astragalus membranaceus, and Citrus medica to water is 1:

10.

5. The preparation method according to claim 3, characterized in that, The soaking time in step 1) is 2 hours.

6. The preparation method according to claim 3, characterized in that, Step 2) The conditions for decocting include: decocting twice, 1 hour each time.

7. The preparation method according to claim 3, characterized in that, Step 2) The drying conditions include: combining the decoctions obtained from two decoctions and filtering them, concentrating the filtrate to obtain an extract, and drying the extract at 40°C to a constant weight to obtain the composition.

8. The use of the composition according to claim 1 or 2 in the preparation of products that improve the body's immune function.

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