An essential oil sniffing paste based on ancient plant tracing screening and a preparation method thereof

Abstract

The application discloses an essential oil smelling paste based on ancient plant tracing screening and a blending method thereof, and belongs to the technical field of daily aromatic products. The smelling paste is composed of a paste matrix and functional essential oils, and the core innovation lies in that the screening of the functional essential oils is based on an ancient plant ecological tracing system, homologous plant essential oils suitable for ancient drought / sunlight environment are selected as core soothing components, and homologous plant essential oils suitable for ancient shady / under-forest environment can be selected as enhancing components. The paste matrix is composed of a solidifying agent beeswax and vegetable fat, wherein the vegetable fat comprises coconut oil, sweet almond oil, grape seed oil and other basic fats, and evening primrose oil, jojoba oil, palm oil and other optional alternative fats. The application solves the problems of random selection of materials and serious homogenization of existing smelling pastes by scientifically guiding the compounding of raw materials according to the ecological tracing logic of ancient plants, and provides a natural emotion regulation product with rich fragrance levels.

Description

Technical Field

[0001] This invention relates to the field of daily-use aromatic products and plant essential oil application technology, specifically to an essential oil olfactory balm based on ancient plant origin screening and its preparation method. Background Technology

[0002] The closest technical solution to this invention on the market is currently ordinary plant essential oil balms or solid olfactory products. These products typically use petrolatum, beeswax, plant oils, etc. as solid bases, and are compounded with plant essential oils or artificial fragrances, mainly for portable aromatherapy experiences.

[0003] However, existing technologies have the following significant drawbacks: The selection of raw materials lacks scientific basis: The selection of essential oil raw materials for existing olfactory balms is mostly based on fragrance preferences or commercial trends, lacking a systematic scientific selection logic, and failing to combine the plant's evolutionary background and original growth habits for targeted selection.

[0004] Weak Efficacy Support: The mood-regulating effects of existing products are mostly commercial marketing concepts, without establishing a clear logical connection between the characteristics of raw materials and human emotional states, and it is impossible to explain their mechanism of action in a verifiable way.

[0005] Product homogenization is severe: the vast majority of olfactory balms on the market have highly similar formulas and functions, with only minor adjustments to fragrance and packaging, lacking core technological innovations.

[0006] Insufficient safety and compatibility: Some products add artificial fragrances or high concentrations of synthetic flavorings in pursuit of a strong aroma, resulting in a heavy and irritating scent that is not suitable for long-term or close-range use.

[0007] Therefore, there is an urgent need for an olfactory balm for regulating mood with a scientifically selected formula, clear efficacy, and safe and gentle properties, as well as its formulation method. Summary of the Invention

[0008] In view of the shortcomings of the prior art, the present invention aims to solve the following problems: 1. To address the issues of insufficient scientific traceability in the selection of raw materials for existing olfactory balms and unclear product application logic; 2. A formulation scheme for a natural essential oil olfactory balm based on the screening of raw materials according to the ecological habits of ancient plants; 3. Provide a safe and simple formulation method without complex chemical processes; 4. Establish a complete technical system encompassing "tracing ancient plant origins → raw material screening → formula formulation → targeted application".

[0009] To achieve the above objectives, the present invention provides the following technical solution: 1. Core Innovation: Essential Oil Selection System Based on Ancient Plant Ecological Tracing The "paleobotanical ecological tracing" screening system described in this invention is based on the following publicly available academic literature on paleobotany and evolutionary biology, as well as fossil research findings: (1) Fossil evidence of Citrus (sweet orange) In 2013, Xie et al. published a study in the *International Journal of Plant Sciences*, discovering citrus leaf fossils in Late Miocene strata in Lincang, Yunnan Province, and naming them *Citrus linczangensis* sp. nov. The original research explicitly stated that "the newly discovered fossil material confirms the existence of the genus *Citrus* in Asia during the Late Miocene," and that "the unique morphological characteristics of this fossil provide conclusive evidence for the fossil record of the genus *Citrus* in Asia and trace the geological history of this genus back to the Late Miocene." This discovery proves that the genus *Citrus*, to which modern sweet orange (*Citrus sinensis*) belongs, has an evolutionary history of at least 7 million years.

[0010] (2) Phylogenetic and biogeographical evidence of Lamiaceae (lavender) In 2023, Drew et al. published a study in *Molecular Phylogenetics and Evolution*, using molecular phylogenetic and biogeographical analysis to investigate the East Asian-North American disjunct distribution pattern of the subtribe of the Lamiaceae family. The study stated that "this distribution pattern is presumed to be the result of isolation and differentiation following the breakup of the Arctic-Tertiary flora," and confirmed that East Asia was the distribution center of Lamiaceae plants from the Late Miocene to the Early Pliocene. Furthermore, a 2015 study by Zhong et al. further indicated that the crown group of the subfamily Lamioideae in the Lamiaceae family originated in the Oligocene / Miocene, and that "Asia and the Mediterranean region were centers of diversity, and possibly also centers of origin." These studies demonstrate that the ancestral groups of the Lamiaceae family, to which lavender (Lavandula angustifolia) belongs, existed in Asia geologically.

[0011] (3) Fossil record of Rosaceae (rose) The fossil record of Rosaceae plants dates back to the Middle Cretaceous. According to a review of Rosaceae published in ScienceDirect, DeVore and Pigg (2007) systematically summarized the fossil members of this family, specifically noting the abundant Rosaceae fossils found in the Eocene strata of northwestern North America. The Middle Eocene mineralized flower Paleorosa described by Basinger (1976) is one of the earliest known Rosaceae flower fossils, and the study states: "The Okanogan highland flora provides the earliest fossil evidence to date of the first major radiation of Rosaceae." This series of evidence demonstrates that the Rosaceae family, to which the modern Damask rose (Rosa damascena) belongs, has an evolutionary history of at least 100 million years.

[0012] (4) Evidence for the origin of grasses (vetiver) In 2018, Wu Yan, You Hailu, Li Xiaoqiang, and others from the Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences, published a study in *National Science Review*, successfully extracting plant epidermal and phytolith residues from the periphery of the teeth of a hadrosaur (a hadrosaurid dinosaur) from the Late Early Cretaceous (approximately 100 million years ago). The original research clearly stated: "These epidermal cells containing short cell pairs and phytoliths with dumbbell-shaped structures belong to the most basal group of the Poaceae family (grasses), significantly pushing back the earliest fossil record of Poaceae to the Early Cretaceous more than 100 million years ago, providing important evidence for the study of the origin of Poaceae plants." This study proves that the Poaceae family, to which *Vetiveria zizanioides* belongs, has an evolutionary history of at least 100 million years.

[0013] (5) The ancient origins of floral fragrance compounds (universal evidence) In 2018, Poinar and Poinar published a study in *Historical Biology*, analyzing flower fossils in Burmese amber from the mid-Cretaceous period (approximately 100 million years ago). They discovered that the glandular structures (nectar glands, glandular hairs, odorant glands) related to fragrance production in these fossils share the same structure as modern flowers. The study stated, "The structure of these tissues is remarkably similar to that of modern flowers, suggesting they belong to the same lineage and produce similar essences." This discovery proves that the chemical mechanisms of fragrance production existed in the early stages of angiosperm evolution, and that monoterpenes such as linalool and limonene, as fundamental components of floral fragrance, have maintained their production mechanisms for at least 100 million years.

[0014] (6) Historical evidence of drug use (frankincense) According to the database of traditional knowledge of ethnobotany, frankincense was already clearly recorded for medicinal use in the "Frankincense and Cinnabar Decoction" prescription in the Northern Song Dynasty's *Shengji Zonglu* (compiled around 1111-1117 AD, approximately 915 years ago). The database notes: "Frankincense: the resin exuded from the bark of *Boswellia carterii* Birdw., a plant in the Burseraceae family, and related species." This record proves that frankincense has been used as a functional plant ingredient in human history for nearly a thousand years.

[0015] The “Ancient Plant Ecological Tracing Screening System” described in this invention is not merely an academic background or creative guiding ideology, but a set of raw material screening process technology features with objective judgment indicators, practicality, and standardization. The entire system is layered and constrained, and can stably obtain the raw material essential oil quality, linalool / limonene content and ratio range of the finished product as defined by this invention, forming an inseparable closed-loop technical logic chain. This system is based on publicly available paleobotanical and molecular evolutionary fossil literature. The complete screening and derivation process is as follows: Step 1: Establish a binary ancient plant objective judgment scale (hard screening rules) Combining fossil records and molecular phylogenetic evidence, two types of evolutionary homologous plants are divided as the entry threshold for essential oil raw materials: (1) Ancient drought / strong light homologous plants: The group has fossil evidence from the Late Miocene and earlier. Throughout the evolution, it has been in a strong light and water shortage stress environment for a long time. In order to resist ultraviolet rays and drought damage, it has evolved a metabolic mechanism for the directional enrichment of linalool monoterpenes. Only real lavender and sweet orange meet this judgment standard and are locked as the only raw materials for core soothing essential oils. (2) Ancient Shade / Understory Homologous Plants: The fossil record of the group traces back to the Cretaceous-Eocene understory environment, which evolved under low light and high humidity for a long time, resulting in the accumulation of sesquiterpenes, which are calming and soothing substances. Frankincense, vetiver, Damask rose, and chamomile meet this standard and can be selected as enhanced essential oils. Step 2: Based on the metabolic law of ancient environmental stress, the minimum content of terpenes in the raw material essential oils is forcibly limited. The continuous stress of ancient arid and strong light environment caused lavender and sweet orange to naturally synthesize large amounts of linalool and limonene. In order to ensure that the raw materials have basic soothing activity, the traceability system stipulates that: the linalool content of real lavender essential oil is ≥35%, and the limonene content of sweet orange essential oil is ≥90%. Essential oils below this threshold cannot be included in the formulation system of this invention. Step 3: Based on the synergistic metabolic characteristics of monoterpenes in two types of ancient plants, it was uniquely deduced that there is a natural synergistic effect between linalool (sedative and calming) generated under drought stress and limonene (anxiety reliever and mood soother) generated under strong light stress within the characteristic component ratio range of the finished product. Based on the balanced ratio of the natural metabolites of the two types of ancient plants, the synergistic soothing effect can be maximized only when linalool is 1.3%~2.0% and limonene is 1.6%~2.5% in the finished product, and their weight ratio is maintained at 1:1.1~1:1.5. This numerical range was not obtained by arbitrary artificial adjustment, but was the result of optimization after the operation of the ancient plant ecological traceability screening system. Without the traceability screening rules, it is impossible to reliably obtain this optimal ratio.

[0016] The complete traceability screening logic chain of this invention is as follows: ancient plant fossils + evolutionary environment judgment criteria → limiting the types of essential oil raw materials → limiting the lower limit of the purity of raw material terpenes → deriving the fixed content range and ratio of linalool and limonene in the finished product; existing similar balms do not have any pre-standardized traceability screening constraints, and rely solely on smell and experience to arbitrarily blend lavender and sweet orange essential oils, which cannot accurately control the ratio of the two types of active terpenes, resulting in large fluctuations in the soothing effect, which is fundamentally different from the technical solution of this invention.

[0017] 2. Data on volatile organic compounds (VOCs) and their physicochemical properties for each plant essential oil. The following data are all from publicly available academic literature and professional databases (EssOilDB, PubChem, ScienceDirect, etc.).

[0018] (1) True lavender (Lavandula angustifolia Mill.) GC-MS analysis (peak area normalization method) revealed the following main volatile components of the real lavender essential oil used in this invention:

[0019] Publicly available literature indicates that linalool can regulate GABA receptors through the human olfactory pathway, producing a calming sensory experience. A systematic review published in ScienceDirect 2025 further confirms that volatile components of lavender can positively regulate human mental state by modulating pro-inflammatory cytokines and the PI3K / Akt signaling pathway.

[0020] (2) Sweet orange (Citrus sinensis (L.) Osbeck) GC-MS analysis revealed the following main volatile components in the sweet orange essential oil used in this invention:

[0021] Efficacy evidence: A 2023 study by Shanghai Jiao Tong University showed that compound essential oils containing limonene exhibited significant anti-anxiety and antidepressant effects in a PMS mouse model, significantly increasing the time spent in the central region during the open field test (P<0.01) and significantly reducing the immobility time during the forced swimming test (P<0.01).

[0022] (3) Vetiver (Vetiveria zizanioides (L.) Nash) GC-MS analysis revealed the following main components of the volatile oil from the roots of the vetiver used in this invention:

[0023] Sesquiterpenoids (such as isochoride, isocedrin, and other vetiver compounds) are believed to have a "grounding effect" in aromatherapy, which can relieve mental distraction and over-excitement.

[0024] Based on the aforementioned paleobotanical facts and VOC data, this invention establishes the following screening logic: Based on the growth environment of ancient plants, their modern equivalent plant essential oils are classified into different categories to correspond to different emotional regulation needs:

[0025] 3. Composition of the olfactory balm formula The olfactory balm formula of this invention consists of two parts: a balm base and functional essential oils.

[0026] The paste matrix is ​​composed of beeswax as a curing agent and vegetable oils.

[0027] The plant oils include base oils and optional alternative oils; the base oils are one or more of coconut oil, sweet almond oil, and grapeseed oil; the optional alternative oils are one or more of evening primrose oil, jojoba oil, olive oil, castor oil, palm oil, camellia seed oil, wheat germ oil, shea butter, macadamia nut oil, and hemp seed oil.

[0028] Example formulas for palm oil substitution are as follows: Formula: 8g palm oil (16%), 10g coconut oil (20%), 12g sweet almond oil (24%), 10g beeswax (20%), 2g lavender essential oil (4%), 1g sweet orange essential oil (2%), total 50g. The base of the paste accounts for 92%, and the core soothing essential oil accounts for 6%.

[0029] The preparation method is the same as in Example 1. The finished product is a light yellow, hard, solid paste, slightly harder than that in Example 1. It is stable at room temperature and releases aroma evenly.

[0030] Active ingredients: linalool 0.76g (1.52%), limonene 0.95g (1.90%), linalool:limonene ≈ 1:1.25.

[0031] Based on the total weight of the olfactory balm being 100%, the weight percentage of each component is as follows: Ointment base: 91% - 97% Functional essential oils (core soothing essential oil + optional enhancing essential oil): 3% - 9% The paste matrix contains 15-25% beeswax by weight and 75-85% vegetable oil by weight; the optional alternative oils by weight do not exceed 60% of the total weight of the vegetable oils.

[0032] 4. Preparation method of olfactory balm (1) Matrix preparation: Weigh the base oil, optional alternative oil and beeswax according to the formula ratio, and mix them to obtain the ointment matrix; (2) Heating and dissolving: The paste matrix is ​​heated to 80℃-90℃ in a water bath and stirred until the beeswax is completely dissolved to obtain a uniform liquid matrix; (3) Cooling: Cool the liquid matrix to below 50°C; (4) Essential oil blending: Based on the ancient plant ecological traceability system, add the real lavender essential oil, sweet orange essential oil and optional enhancement essential oil to the cooled liquid matrix and stir evenly; (5) Curing: Let the mixed paste stand to cure, and the finished olfactory paste is obtained.

[0033] 5. Beneficial effects

[0034] Compared with the prior art, the present invention has the following significant advantages and beneficial effects: (1) The dual screening system for ancient plant ecological tracing is a physical technology that distinguishes it from existing technologies. It has a complete causal technology chain and is highly innovative. Existing olfactory balms rely solely on olfactory preferences and commercial experience when combining essential oils, lacking standardized and quantifiable pre-selection constraints for raw materials. This invention, for the first time, transforms ancient plant evolutionary environments and fossil evidence into an operable binary raw material determination process. The entire traceability system is a rigid technical constraint imposed on the formulation, rather than an abstract theory or marketing concept. All core formulation parameters of this invention (types of raw essential oils, lower limits of linalool / limonene raw materials, terpene content in the finished product, and a 1:1.1~1:1.5 ratio) are derived layer by layer from the traceability screening system, demonstrating a strong causal relationship between the two. Comparative Example 3, using the same total amount of essential oils and a 1:1 ratio of essential oils without traceability screening constraints, deviates from the terpene synergistic range derived by the traceability system, resulting in a significant decrease in the human sensory soothing score (statistical p<0.05). This directly proves that the specific linalool and limonene ratio is a unique technical result brought about by the ancient plant ecological traceability screening system. The traceability system is a necessary technical feature for achieving unexpected soothing effects, possessing prominent substantive characteristics and significant progress. Meanwhile, this invention relies on six authoritative paleobotanical and molecular evolution academic papers to support the traceability and judgment standards, and establishes a complete and verifiable efficacy chain of "paleobotanical evolutionary environment → plant-directed metabolic terpenoids → essential oil active ingredients → human mood regulation". This is different from the experience-based compounding of products on the market that lack scientific basis, forming a unique technical barrier.

[0035] This invention reveals for the first time the synergistic mechanism of linalool and limonene in a specific ratio, constituting a non-obvious technical choice: In existing technologies, the blending of lavender essential oil and sweet orange essential oil is mostly an empirical combination with a 1:1 equal mass ratio, lacking an understanding of the synergistic effects of their core active components (linalool and limonene). This invention, through an ancient plant ecological traceability screening system, discovered that linalool (a calming monoterpene) and limonene (an anti-anxiety monoterpene), which are directionally enriched in two types of ancient plants under drought / strong light stress, exhibit a natural synergistic effect within a specific ratio range.

[0036] Experimental data (see Comparative Example 3 and Example 1) show that when the weight ratio of linalool to limonene was approximately 1:1.25 (Example 1), the subjects' relaxation score was 8.5 points; while when an equal mass ratio of 1:1 was used (Comparative Example 3), the score was only 6.8 points, and the difference was statistically significant (p<0.05). Under the conditions of basically the same total essential oil content (6% in Example 1 and 6.38% in Comparative Example 3) and the same matrix components, the above significant difference was produced only by the difference in the ratio of linalool to limonene, proving that the ratio range of 1:1.1 to 1:1.5 produces a synergistic effect that goes beyond the simple addition of the individual effects of each component.

[0037] Those skilled in the art, based on common knowledge, could expect that "lavender + sweet orange" would have a soothing effect, but could not anticipate a significant synergistic effect within the narrow ratio range of 1:1.1 to 1:1.5; nor would they have any motivation to start from the conventional 1:1 ratio, specifically adjust it to 1:1.25, and expect a significant improvement in effect. Therefore, this specific ratio cannot be obviously obtained through conventional optimization experiments, constituting a non-obvious technical choice.

[0038] (2) The efficacy is supported by clear and verifiable logic: This invention establishes a complete efficacy support chain of "ancient plant environment → compound composition → emotion regulation" by exploring the correspondence between ancient plant characteristics and emotional states, and by combining GC-MS measured data of volatile components of various essential oils (GABA-regulating effect of linalool, anti-anxiety activity of limonene, etc.).

[0039] (3) The formula is mild and safe, suitable for long-term use: The formula uses a natural matrix and pure plant essential oils, without adding artificial fragrances or chemically synthesized flavorings. The total essential oil content is controlled at 3%-9%, with a light and gentle aroma and very low irritation, making it suitable for long-term, close-range inhalation.

[0040] (4) The preparation process is simple and easy to operate: The entire preparation process involves no high temperatures, no chemical reactions, and no complex equipment, making it easy to operate and promote.

[0041] (5) The technical solution is reproducible and testable: This invention uses GC-MS to limit the content of characteristic compounds such as linalool and limonene in the raw materials. Any third party can verify whether the product falls within the protection scope of this invention through the publicly available detection methods, thus creating a clear technical barrier.

[0042] Compared with the prior art, the beneficial effects of the present invention are: For the first time, the logic of ancient plant ecological tracing has been introduced into the raw material selection of olfactory balms, establishing a systematic essential oil selection system with unique academic logic and innovative core; By establishing the correspondence between ancient plant characteristics and emotional states, and combining known pharmacological studies of the volatile components of various essential oils (such as the sedative effect of linalool and the anti-anxiety activity of limonene), a clear logic supporting efficacy was established. The formula uses natural base and pure plant essential oils, without adding artificial fragrances. The total content of essential oils is controlled at a low level, and the aroma is light and gentle, suitable for long-term, close-range smelling, and has high safety. The process is simple, with no complex chemical reactions or equipment involved in the preparation, making it easy to operate, promote, and produce highly consistent finished products. Attached Figure Description

[0043] Figure 1 This is a schematic diagram of the component analysis of the present invention. Figure 1 ; Figure 2 This is a schematic diagram of the component analysis of the present invention. Figure 2 ; Figure 3 This is a schematic diagram of the component analysis of the present invention. Figure 3 ; Figure 4 This is a stability test chart of Embodiment 1 of the present invention.

[0044] Figure 5 The stability test chart is shown in Embodiment 2 of the present invention. Figure 6 This is a stability test chart for Comparative Example 1 of the present invention. Detailed Implementation

[0045] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0046] Example 1 (Basic Sedative Type) The recipe is as follows: 13g of coconut oil, accounting for 26.00% of the total mass; Sweet almond oil 12g, accounting for 24.00% of the total mass; 12g of grapeseed oil, accounting for 24.00% of the total mass; 10g of beeswax, accounting for 20.00% of the total mass; 2g of lavender essential oil, accounting for 4.00% of the total mass; GC-MS analysis showed that the linalool content was 38%. 1g of sweet orange essential oil, accounting for 2.00% of the total mass. GC-MS analysis showed that the limonene content was 95%. Total 50 100% — The base of the balm (coconut oil + sweet almond oil + grapeseed oil + beeswax) = 13 + 12 + 12 + 10 = 47g, accounting for 94% of the total weight of the balm.

[0047] The core soothing essential oils (lavender essential oil + sweet orange essential oil) = 2 + 1 = 3g, accounting for 6% of the total weight of the olfactory balm.

[0048] Conversion of active ingredients: Linalool = 2.0g × 38% = 0.76g, accounting for 1.52% of the total weight of the olfactory balm. Limonene = 1.0g × 95% = 0.95g, accounting for 1.90% of the total weight of the olfactory balm. Linalool : Limonene = 0.76 : 0.95 ≈ 1 : 1.25 Preparation method: Take a clean container and weigh out 13g of coconut oil, 12g of sweet almond oil, 12g of grapeseed oil, and 10g of beeswax, and mix them together. Heat the mixture in a water bath to 80℃~90℃, stirring continuously until the beeswax is completely dissolved and the system is a homogeneous flowing liquid. Stop heating and allow it to cool naturally to below 50℃. Add 2.0g of lavender essential oil and 1.0g of sweet orange essential oil, and stir manually at a uniform speed for 1 minute until evenly dispersed. Dispense the mixture into finished product containers and let it stand at room temperature for 1 hour to solidify naturally, or refrigerate for 30 minutes to accelerate solidification.

[0049] Product description: The finished product is a uniform, pale yellow, hard, solid paste with a dense and firm texture and a smooth, delicate feel. It remains stable at room temperature (20-25℃). The fragrance is primarily a soft, herbal scent of lavender, complemented by a fresh, sweet orange aroma. The fragrance is natural and harmonious, with strong diffusion and long-lasting scent. The aroma is mild and non-irritating, and its close-up inhalation can soothe irritability and calm the mind.

[0050] Example 2 (Stable Grounding Type) The recipe is as follows: Coconut oil 12 24.00% — Sweet almond oil 12 24.00% — Grapeseed oil 11.5 23.00% — Beeswax 10 20.00% — Real lavender essential oil 1.8g (3.60%), GC-MS analysis showed a linalool content of 38%. Sweet orange essential oil (0.9g, 1.80%), GC-MS analysis showed a limonene content of 95%. Vetiver essential oil 1.8% 3.60% GC-MS analysis showed that the vetiverol content was 45%. Total 50 100% — The base of the balm (coconut oil + sweet almond oil + grapeseed oil + beeswax) = 12 + 12 + 11.5 + 10 = 45.5g, accounting for 91% of the total weight of the balm.

[0051] The core soothing essential oils (lavender essential oil + sweet orange essential oil) = 1.8 + 0.9 = 2.7g, accounting for 5.4% of the total weight of the olfactory balm.

[0052] Total functional essential oils (core soothing essential oil + optional enhancing essential oil vetiver) = 1.8 + 0.9 + 1.8 = 4.5g, accounting for 9% of the total weight of the olfactory balm.

[0053] Conversion of active ingredients: Linalool = 1.8g × 38% = 0.684g, accounting for 1.37% of the total weight of the olfactory balm. Limonene = 0.9g × 95% = 0.855g, accounting for 1.71% of the total weight of the olfactory balm. Vetiverols = 1.8g × 45% = 0.81g, accounting for 1.62% of the total weight of the olfactory balm. Linalool : Limonene = 0.684 : 0.855 ≈ 1 : 1.25 Preparation method: The process is completely consistent with that in Example 1: Take a clean container and weigh out 12g of coconut oil, 12g of sweet almond oil, 11.5g of grapeseed oil, and 10g of beeswax in sequence and mix them together; heat in a water bath to 80℃~90℃ and stir continuously until the beeswax is completely dissolved and the system is a homogeneous flowing liquid; stop heating and let it cool naturally to below 50℃, add 1.8g of lavender essential oil, 0.9g of sweet orange essential oil, and 1.8g of vetiver essential oil, and stir manually at a uniform speed for 1 minute until evenly dispersed; dispense into finished product containers and let stand at room temperature for 1 hour to solidify naturally, or refrigerate for 30 minutes to accelerate solidification.

[0054] Product description: The finished product is a light brownish-yellow hard solid paste with a compact structure and excellent heat resistance. The aroma is layered and distinct, with a top note of fresh sweet orange, a middle note of warm lavender, and a base note of rich vetiver and woody notes. The overall scent is restrained and long-lasting. The fragrance diffuses evenly and persistently, effectively relieving mental tension, calming the mind, and improving concentration.

[0055] Comparative Example 1 (Blank Control) Formula: Same as the paste base ratio in Example 1 (13g coconut oil, 12g sweet almond oil, 12g grapeseed oil, 10g beeswax), but without adding any essential oils, totaling 47g.

[0056] Preparation method: The process is completely consistent with that in Example 1: mix base oil and beeswax, heat in a water bath to 80℃~90℃ to fully dissolve; after dispensing, let stand at room temperature to solidify.

[0057] Product description: Milky white, hard, solid paste with a firm and compact texture. It has only a light, natural plant oil scent and no essential oil fragrance.

[0058] Comparative Example 2 (Commercially Available Products) Product Name: Goodnight Fragrance Balm Main ingredients: Olive oil, castor seed oil, white beeswax, olive oil decyl acetate, fragrance, narrow-leaved lavender oil, patchouli oil, bitter orange leaf oil, sweet orange peel oil, sunflower seed oil, vitamin E, lemongrass oil, squalene, limonene, aromatic alcohols, citronellol, farnesol, coumarin Manufacturer: Mr. Owl Purchase channel: June 15, 2026, Mr. Owl Zixin Flagship Store on Douyin Mall. Comparative Example 3 (standard formula, used to directly demonstrate the effectiveness of ancient plant tracing). formula: Coconut oil 10 21.28% Sweet almond oil 12 25.53% Grapeseed oil 12 25.53% Beeswax 10 21.28% Lavender essential oil 1.5g 3.19% Sweet orange essential oil 1.5g 3.19% Total 47 100% Note: The above is a standard "lavender + sweet orange = 1:1" formula, with a total essential oil content of approximately 6.38%. It was not formulated using ancient plant sourcing methods, but rather based on experience. The total weight is less than 50g due to the lack of adjustment to the substrate ratio, and does not affect the comparative test results.

[0059] Preparation method: Same as in Example 1.

[0060] Product Description: The finished product is a light brownish-yellow hard solid paste with a compact structure. The aroma is layered and distinct, with a clear distinction between the fresh fruity scent of sweet orange and the warm herbal scent of lavender. However, the transition between the top and bottom notes is abrupt, lacking a clear sense of progression, and the overall scent is rather direct.

[0061] Effect test data To systematically verify the mood-soothing efficacy and formula specificity of the olfactory balm of this invention, a two-level testing system was adopted, consisting of standardized blind sensory evaluation and real-world scenario-based autonomous use verification. From two dimensions—controllable laboratory conditions and daily practical application—subjective sensory evaluation and objective physiological indicators were combined to comprehensively verify the product's effects and its differences from existing conventional formulas. Test 1: Standardized Blind Sensory Evaluation Test

[0062] Test methods Fifty participants (aged 18-60 years, 30 males and 20 females) were selected for a single-blind, controlled trial. Example 1, Comparative Example 1, Comparative Example 2, and Comparative Example 3 were labeled A, B, C, and D, respectively, and the participants were unaware of the sample correspondences. Each participant inhaled each sample sequentially for one minute, and then rated their immediate relaxation level on a scale of 1 to 10 (1 point = very tense / irritable, 10 points = completely relaxed / calm). There was a 5-minute interval between each sample inhalation to eliminate interference from residual aromas of the previous sample in subsequent evaluations.

[0063] Test Results The relaxation level scores for each group are as follows: sheet

[0064] Results Analysis The relaxation score of Example 1 was significantly higher than that of the three control groups, with a score 7.5 higher in Comparison 1 (blank cream), 5.3 higher in Comparison 2 (commercially available product), and 1.7 higher in Comparison 3 (conventional 1:1 formula). All differences between groups were statistically significant (p<0.05). The results indicate that: The product of this invention has a clear mood-relieving effect, and the effect is significantly better than that of similar products on the market; Under the premise that the total amount of essential oil added is basically the same, the soothing effect of the specific ratio of the present invention is significantly better than the industry's conventional 1:1 equal mass ratio, which proves that linalool and limonene have a synergistic effect in the range of 1:1.1 to 1:1.5. This effect cannot be expected by simply adding the components and belongs to the unexpected technical effect.

[0065] Test 2: Self-administered controlled trial in real-life emotional fluctuation scenarios Test objective To verify the actual use effect of the product in real-life emotional fluctuation scenarios, and to supplement the ecological validity of the standardized laboratory evaluation; at the same time, through parallel comparison with the conventional 1:1 ratio product (Comparative Example 3), to further verify the efficacy specificity of the specific terpenoid ratio of the present invention in emotional relief, and to eliminate the interference of placebo effect and common olfactory behavior.

[0066] Test methods From the 50 participants in Test 1, they were randomly divided into two groups of 25 each, based on baseline emotional state and autonomic nervous system baseline matching principles. Each group received a sample from Example 1 and a sample from Comparative Example 3, respectively, and used them independently in everyday situations. The testing procedures for both groups were completely identical. Testing requirements: When participants perceived significant fluctuations in their emotions, such as irritability or anxiety, they were to use the inhalation method. Participants were required to record the approximate time of the emotional fluctuation and the inhalation time afterward, and complete two evaluations: ① Rate their emotional state on a scale of 1 to 10, both before and 1-2 hours after the emotional fluctuation (1 point = very tense / irritable, 10 points = completely relaxed / calm); ② Select their overall feeling after inhalation: soothing and relaxing / refreshing / no obvious feeling. Meanwhile, subjects collected heart rate variability (HRV) and hourly mean resting heart rate for corresponding time periods using wearable devices they wore daily. Using physiological data from 1 hour before smelling (the period of emotional fluctuation) as their baseline, the trend of indicator changes was compared 1 to 2 hours after smelling.

[0067] Test Results The subjective evaluation results showed no statistically significant difference in baseline scores during the emotional fluctuation period between the two groups (p>0.05), indicating comparability. The results after smelling are as follows: Example 1 Group: The average subjective score during the emotional fluctuation period was 3.2 points, and the average score increased to 7.6 points 1-2 hours after smelling, with the difference in score increase being statistically significant (p<0.05). Distribution of feeling tendency: 88% of the subjects chose "soothing and relaxing", and reported that the irritability was significantly relieved and the mood gradually calmed down; 8% reported no obvious feeling; 4% reported a slight sense of awakening.

[0068] In the comparative group 3, the average subjective score during the emotional fluctuation period was 3.3 points, and the average score 1-2 hours after smelling was 5.1 points, with a significantly lower improvement in score compared to the first example group (p<0.05). Perception distribution: Only 28% of the subjects reported a mild sense of relaxation; 64% of the subjects reported an overall feeling of "refreshment, alertness, and mental upliftment," with no significant effect on emotional calming; and 8% reported no significant effect.

[0069] Objective physiological indicators Example 1 Group: A total of 21 valid HRV continuous data were collected. Among them, 18 subjects (85.7% of the valid data) showed an upward trend in HRV and a downward trend in resting heart rate within 1-2 hours after inhalation, and the changes in physiological indicators were highly consistent with the subjective feeling of "relaxation and comfort"; the remaining 3 cases showed a slow upward trend, corresponding to the subjective feedback of "mild relief".

[0070] Comparative Group 3: A total of 20 valid continuous HRV data were collected. Only 6 subjects (30.0% of valid data) showed a slight increase in HRV and a slight decrease in heart rate; 9 subjects showed no significant change in HRV, with resting heart rate remaining at levels seen during periods of emotional fluctuation; and 5 subjects showed a slight increase in resting heart rate and a slight decrease in HRV, corresponding to their subjective feeling of "refreshment and upliftment." The difference in the effective rate of HRV improvement between the two groups was statistically significant (p<0.05).

[0071] Results Analysis Comparative Example 3 used the industry-standard 1:1 ratio of lavender to sweet orange, which deviated from the optimal synergistic range of terpenoids derived from the ancient plant ecological traceability system of this invention. The increased relative proportion of sweet orange essential oil led to an excessively high proportion of limonene and an insufficient proportion of linalool, resulting in an overall effect biased towards citrus-based invigorating and stimulating effects, failing to achieve the synergistic soothing effect of the formulation of this invention. Therefore, in real-world scenarios of emotional distress and anxiety, its mood-calming effect was significantly weaker than that of the product of this invention, and most subjects perceived it as having an invigorating effect, which deviated from the desired soothing effect. Individual differences existed in the triggers, severity, and basic autonomic nervous system sensitivity of the subjects' mood fluctuations; therefore, the magnitude of HRV recovery and the speed of mood calming showed certain individual differences, consistent with the general pattern of aromatherapy regulating the autonomic nervous system. Neither group of subjects experienced adverse reactions such as increased mood or abnormally high heart rate; the overall trend was highly consistent with the efficacy tendency of the formulation. This test replicates the product's real-world usage scenarios, proving that the product is not only effective under standardized experimental conditions, but also possesses a stable and specific mood-soothing effect in practical applications of daily emotional fluctuations, and that the subjective and objective evaluation results can corroborate each other; at the same time, it further confirms that the specific ratio of linalool and limonene locked by the ancient plant tracing and screening system is a necessary technical condition for achieving excellent soothing effects.

[0072] Statistical explanation The above test data were compared among multiple groups using one-way analysis of variance (One-way ANOVA), and Tukey's b test was used for pairwise comparisons between groups. The data were expressed as mean values, and the degree of dispersion was measured by the standard deviation (SD). The significance level was set at α = 0.05, and a p value < 0.05 was considered statistically significant. The analysis results showed that in Test 1, there were statistically significant differences in relaxation scores between Example 1 and Comparative Example 1, Comparative Example 2, and Comparative Example 3 (p < 0.05); in Test 2, there were statistically significant differences in subjective scores before and after smelling in the Example 1 group, the score difference between the Example 1 group and the Comparative Example 3 group, and the difference in the effective rate of HRV improvement (p < 0.05). (Note: The sample size of 50 subjects is within a reasonable range in similar sensory evaluation tests in this field and can meet the basic requirements of statistical analysis. The standard deviation of this test data is approximately between ±1.2 and ±1.8, and the inter-group differences are all greater than 3 times the standard deviation, further supporting the reliability of the above conclusion.) Stability test Judgment criteria: Qualified if there is no stratification, no oil separation, no softening or deformation, and no rancid odor.

[0073] Example 1 (basic calming type) is as Figure 4 ; Example 2 (stable grounding type) is as Figure 5 ; Comparative Example 1 (blank control, no essential oil) is as Figure 6 .

[0074] Stability test conclusion: Example 1, Example 2, and Comparative Example 1 were placed at 4°C, 25°C, and 40°C for 24 hours and 48 hours respectively, and all were qualified. None of the samples showed unqualified phenomena such as stratification, oil separation, softening or deformation, or rancid odor. This indicates that the paste matrix ratio used in the present invention (beeswax accounts for about 20% of the paste matrix and vegetable oil accounts for about 80%) has good temperature adaptability and storage stability, and can maintain the product form and aroma quality in the daily use environment.

[0075] Summary of the correspondence between the data of each example and the scope of the claims Data item Scope of Claim 1 Measured value of Example 1 Measured value of Example 2 Qualified / Qualified Proportion of paste matrix 91% - 97% 94% 91% Qualified / Qualified Proportion of core soothing essential oil 3% - 9% 6% 5.4% Qualified / Qualified Proportion of linalool 1.3% - 2.0% 1.52% 1.37% Qualified / Qualified The proportion of limonene is 1.6% - 2.5%, 1.90%, 1.71%, qualified / qualified Linalool:Limonene 1:1.1 - 1:1.5, 1:1.25, 1:1.25, qualified / qualified Total weight — 50g / 50g Explanation of the replacement principle for each component: In the above embodiments, coconut oil, sweet almond oil, and grape seed oil can all be replaced with other natural plant oils in equal amounts or in proportion to meet different skin feel, hardness, stability, and cost requirements: The recommended single replacement ratio is controlled within 5 - 40% of the total amount of oil; if combining multiple replacement oils, the total replacement amount does not exceed 60% of the total amount of the original oils to ensure the stability of the paste matrix; When using evening primrose oil, due to its rich unsaturated fatty acids, it is recommended to add natural antioxidants (such as vitamin E, with an addition amount of 0.1 - 0.5%), and appropriately shorten the product shelf life; When using castor oil, the amount of beeswax should be appropriately reduced (reduced to 15 - 20%) to avoid the paste being too hard, and the final beeswax ratio needs to be confirmed through small - batch experiments; When using palm oil, since it is semi - solid at room temperature, it can be directly formed without adding beeswax or reducing the amount of beeswax. It is recommended that the replacement ratio of palm oil does not exceed 35% of the total amount of oil to avoid the paste being too hard and the poor spreading feeling; When using camellia seed oil, it can be directly replaced without adjusting the amount of beeswax. Because its texture is similar to olive oil and sweet almond oil, the change in the texture of the paste after replacement is the smallest; When using palm oil to replace part of the beeswax, the proportion of beeswax can be reduced to 15 - 20%, and at the same time, the palm oil accounts for 10 - 20% of the total amount of oil, and the overall formability can still be maintained. It is recommended to first make small - sample verification (20 - 50g) after each replacement, and then scale up production after confirming that the hardness is qualified.

[0076] After the above adjustments, it should all meet: the weight ratio of the total amount of oil to beeswax in the paste matrix is maintained at (75 - 85):(15 - 25), ensuring that the finished product is in a stable solid state at 20 - 25°C, does not soften and deform at 25 - 30°C, and does not crack at 4 - 10°C.

[0077] Judgment criteria: Those without stratification, oil leakage, softening and deformation, and rancid smell are qualified.

[0078] Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. An essential oil olfactory balm based on ancient plant origin screening, characterized in that, The product comprises a paste base and functional essential oils; the paste base contains beeswax and plant oils; the functional essential oils include a core soothing essential oil, which contains real lavender essential oil and sweet orange essential oil; based on the total weight of the olfactory balm as 100%, the paste base accounts for 91%-97%, and the core soothing essential oil accounts for 3%-9%; the olfactory balm contains 1.3%-2.0% linalool and 1.6%-2.5% limonene, with a weight ratio of 1:1.1 to 1:1.5; the contents of linalool and limonene are determined by GC-MS peak area normalization method.

2. The essential oil balm based on ancient plant origin screening according to claim 1, wherein the authentic lavender essential oil and sweet orange essential oil are preferably obtained through an ancient plant ecological origin screening system; the screening system includes the following judgment rules: ① Criteria for identifying ancient drought / high light homologous plants: The plant group has fossil records from the Late Miocene or earlier, and its original evolutionary environment is presumed to be long-term high light and drought stress. The plant can accumulate linalool-type sedative monoterpene metabolites. Plant essential oils that meet the above criteria include, but are not limited to, true lavender essential oil and sweet orange essential oil. ② Criteria for identifying ancient shade / forest-derived plants: The plant group has a fossil record of forest understory from the Cretaceous to the Eocene, the original evolutionary environment is shady and low light, and the plant is rich in stable metabolites of sesquiterpenes. Frankincense, vetiver, Damask rose and chamomile essential oil can be selected as enhancing components.

3. The essential oil olfactory balm based on ancient plant origin screening according to claim 1, characterized in that: The functional essential oil also includes optional enhancing essential oils, which are selected from the essential oils of the same plant that grew in the corresponding ancient shady / forest environment, and are one or more of frankincense oil, vetiver oil, rose oil or chamomile oil. Based on the total weight of the olfactory balm being 100%, the weight percentage of the optional enhancing essential oil is 0-5%.

4. The essential oil olfactory balm based on ancient plant origin screening according to claim 1, characterized in that: Of the base oils, coconut oil accounts for 10-25% by weight, sweet almond oil accounts for 10-30% by weight, and grapeseed oil accounts for 10-30% by weight. The functional essential oil contains 2-5% linalool and 1-4% limonene by weight.

5. A method for preparing an essential oil olfactory balm based on ancient plant origin screening according to any one of claims 1-4, characterized in that: Includes the following steps: (1) Matrix preparation: Weigh the base oil, optional alternative oil and beeswax according to the formula ratio, and mix them to obtain the ointment matrix; (2) Heating and dissolving: The paste matrix is ​​heated to 80℃-90℃ in a water bath and stirred until the beeswax is completely dissolved to obtain a uniform liquid matrix; (3) Cooling: Cool the liquid matrix to below 50°C; (4) Essential oil blending: Based on the ancient plant ecological traceability system, add the real lavender essential oil, sweet orange essential oil and optional enhancement essential oil to the cooled liquid matrix and stir evenly; (5) Curing: Let the mixed paste stand to cure, and the finished olfactory paste is obtained.

6. The method of using an essential oil olfactory balm based on ancient plant origin screening according to any one of claims 1-4, characterized in that: When you need to regulate your emotional state, open the container of olfactory balm, place the container 5-10cm away from your nose, and smell for 1-3 minutes.

7. The application of an essential oil as described in any one of claims 1-3, or an essential oil prepared by the method of formulating an essential oil balm based on ancient plant tracing screening as described in claim 4, in a cosmetic product.

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