GABA-lysine calcium supplementing preparation for promoting calcium absorption

Abstract

The application discloses a GABA-lysine calcium supplementing preparation for promoting calcium absorption, and relates to the technical field of calcium supplementing preparations.The preparation comprises a calcium source modified microsphere component, an absorption promoting inclusion component, a calcium directional deposition component and a synergistic component; the calcium source modified microsphere component is formed by compounding seaweed calcium, calcium hydrogen phosphate, chitosan and a phosphorylation reagent, and is used as a basic calcium source core material of the preparation; the component is subjected to surface modification treatment to form a hydrophilic microsphere level particle structure; a complex inclusion compound is formed by GABA, L-lysine, casein phosphopeptide and carboxymethyl-beta-cyclodextrin, and is used as a core absorption promoting material for calcium intestinal absorption; the calcium source modified microsphere component and the absorption promoting inclusion component are formed into a functional adaptation, so that the calcium source exists in a stable structure and can fully contact with the absorption promoting component, and a basic condition for calcium intestinal absorption is constructed.

Description

Technical Field

[0001] This invention relates to the field of calcium supplementation technology, specifically to a GABA-lysine calcium supplementation that promotes calcium absorption. Background Technology

[0002] Calcium is an essential macro-element for the human body, participating in many key physiological activities such as bone formation, nerve conduction, and muscle contraction. The human body cannot synthesize calcium on its own and must obtain it through diet or exogenous supplements. There are clear calcium supplementation needs at various physiological stages, such as the bone development of adolescents, the nutritional supply during pregnancy, and the calcium loss in the bones of middle-aged and elderly people. With the improvement of public health awareness, calcium supplements have become a research hotspot in the food and pharmaceutical fields, and the market demand for calcium supplements that are suitable for all population groups and have complete functions continues to increase.

[0003] However, the composition design of existing calcium supplements lacks systematicness and specificity. They do not have dedicated calcium source modified core materials, and the calcium source raw materials are simply compounded without structural modification, making it difficult to form a stable particulate structure. The absorption-promoting components are not encapsulated, so they cannot form dedicated absorption-promoting functional materials, which easily leads to the loss of active ingredients. There are no dedicated functional components for calcium deposition in bones. They simply add conventional nutrients, which cannot achieve the targeted action of calcium. The addition of antioxidant components lacks a reasonable ratio basis, and the components of the formulation do not have an effective binding mode, making it difficult to form an integrated structure, and there is no synergistic effect mechanism between the components. Summary of the Invention

[0004] The purpose of this invention is to overcome the shortcomings of existing technologies and provide a GABA-lysine calcium supplement that promotes calcium absorption. This invention uses a calcium-modified microsphere component formed by compounding seaweed calcium, dicalcium phosphate, chitosan, and a phosphorylation reagent. This microsphere serves as the basic calcium source core material of the formulation. Phosphorylation surface modification treatment transforms this component into a hydrophilic microsphere-level particle structure. Simultaneously, GABA, L-lysine, casein phosphopeptide, and carboxymethyl-β-cyclodextrin form a complex inclusion complex, which serves as the core absorption-promoting material for intestinal calcium absorption. The calcium-modified microsphere component and the absorption-promoting inclusion complex are functionally compatible, allowing the calcium source to exist in a stable structure while maintaining sufficient contact with the absorption-promoting components, thus establishing the basic conditions for intestinal calcium absorption.

[0005] To solve the above-mentioned technical problems, the present invention provides the following technical solution: In one aspect, a GABA-lysine calcium supplement that promotes calcium absorption, the supplement comprising, by weight: Calcium-modified microspheres: 60-80 parts; Absorption-enhancing inclusion complex: 15-25 parts; Calcium-directed deposition components: 3-8 parts; Synergistic effect component: 0.05~0.15 parts.

[0006] Furthermore, the calcium source modified microsphere component is a compound of seaweed calcium, dicalcium phosphate, chitosan, and phosphorylation reagent. The mass ratio of seaweed calcium, dicalcium phosphate, chitosan, and phosphorylation reagent in the calcium source modified microsphere component is 80:18~22:9~11:2.8~3.2. The calcium source modified microsphere component is a hydrophilic composite calcium source microsphere obtained by phosphorylation surface modification, exhibiting a microsphere-level particle structure, and serves as the basic calcium source core material for this formulation.

[0007] Furthermore, the absorption-enhancing inclusion component is a complex inclusion compound of GABA, L-lysine, casein phosphopeptide, and carboxymethyl-β-cyclodextrin, with an inclusion rate ≥95%, serving as a core absorption-enhancing material for efficient calcium intestinal absorption. Specifically, the molar ratio of GABA to L-lysine is 1:1.5, the amount of casein phosphopeptide added is 4.8~5.2% of the total mass of GABA and L-lysine, and the mass ratio of GABA, L-lysine, casein phosphopeptide, and carboxymethyl-β-cyclodextrin is 0.8~1.2:1.8~2.2.

[0008] Furthermore, the calcium-directed deposition component is a composition of K2MK7 and natural VD2, exhibiting a uniform lipid-soluble liquid structure, serving as a functional material for calcium-directed bone deposition. The K2MK7 and natural VD2 are extracted from natto and button mushrooms, respectively, with a mass ratio of K2MK7 to natural VD2 of 0.8~1.2:18~22.

[0009] Furthermore, the synergistic component is natural vitamin E, and the amount added is 0.08~0.12% of the total mass of the calcium source modified microsphere component, the absorption-promoting inclusion component, and the calcium-directed deposition component. This synergistic component is an active ingredient antioxidant protective material.

[0010] Furthermore, the formulation is prepared by forming an integrated composite microsphere structure through calcium-modified microsphere components, absorption-enhancing inclusion components, calcium-directed deposition components, and synergistic components. During the preparation of the formulation, an additional gum arabic binder is added. The amount of gum arabic binder added is 1.8~2.2% of the total mass of the calcium-directed deposition components and the absorption-enhancing inclusion components. The gum arabic binder is used to improve the adsorption and binding effect between the components and ensure the integrity of the integrated composite microsphere structure.

[0011] On the other hand, a GABA-lysine calcium supplement that promotes calcium absorption is prepared as follows: Preparation of calcium source microspheres: Algal calcium, dicalcium phosphate, chitosan and phosphorylation reagent are mixed and prepared by emulsification crosslinking method to obtain basic calcium source microspheres. The basic calcium source microspheres are then subjected to surface phosphorylation modification treatment to obtain calcium source modified microsphere components. Preparation of complex inclusion complex: GABA, L-lysine, casein phosphopeptide and carboxymethyl-β-cyclodextrin were mixed and formed into an inclusion structure by saturated aqueous solution method to obtain an absorption-enhancing inclusion component; Preparation of depositional components: K2MK7 was extracted from natto and natural VD2 was extracted from button mushrooms. K2MK7 and natural VD2 were mixed and purified to obtain calcium-directed depositional components. Preparation of inclusion suspension: The calcium-directed deposition component and the absorption-promoting inclusion component are mixed, gum arabic binder is added and the mixture is stirred continuously to form a homogeneous inclusion suspension without particles; Preparation of composite microspheres: The calcium-modified microsphere components are immersed in an inclusion suspension for adsorption treatment, and synergistic components are added and stirred to mix evenly. The integrated composite microsphere formulation is then obtained through a drying process.

[0012] Furthermore, in the preparation of calcium source microspheres, seaweed calcium, dicalcium phosphate, chitosan, and phosphorylation reagent are mixed and then added to deionized water to form a homogeneous suspension. Microspheres are formed in an emulsification device using an emulsification crosslinking method to obtain basic calcium source microspheres with a particle size of 50-80 μm. The surface phosphorylation modification treatment involves placing the formed basic calcium source microspheres in a phosphorylation reagent system for directional surface modification. During the modification process, the microspheres are continuously stirred at a speed of 300-500 r / min for 1-2 h. After modification, the microspheres are washed with deionized water and dried at low temperature to obtain a hydrophilic calcium source modified microsphere component.

[0013] Furthermore, in the preparation of the composite inclusion complex, carboxymethyl-β-cyclodextrin is added to deionized water to prepare a saturated aqueous solution, and then GABA, L-lysine and casein phosphopeptide are added. The mixture is stirred continuously at 200-400 r / min for 2-3 hours in a constant temperature environment of 40-50℃ using the saturated aqueous solution method. After filtration and drying, an absorption-enhancing inclusion component with an inclusion rate of ≥95% is obtained.

[0014] Furthermore, in the preparation of the deposition component, natto and button mushrooms are crushed separately, and K2MK7 and natural VD2 are extracted separately using supercritical CO2 extraction method. The extraction pressure is 30~40MPa, the extraction temperature is 45~55℃, and the extraction time is 2~3h. Then, K2MK7 and natural VD2 are mixed and purified by chromatography to obtain a calcium-oriented deposition component with a purity ≥98%.

[0015] Furthermore, in the preparation of the inclusion suspension, the calcium-directed deposition component and the absorption-promoting inclusion component are mixed and then gum arabic binder is added. The mixture is stirred continuously at a speed of 400~600 r / min until it is homogeneous and free of solid particles, thus obtaining the inclusion suspension.

[0016] Furthermore, in the preparation of the composite microspheres, the adsorption treatment adopts ultrasonic adsorption. The calcium source modified microspheres are immersed in the inclusion suspension for 30 minutes under an ultrasonic power of 200~300W. After adding the synergistic component and mixing, the microspheres are dried by spray drying at an inlet temperature of 160~170℃ and an outlet temperature of 70~80℃ to obtain an integrated composite microsphere formulation with uniform particle size.

[0017] Compared with existing technologies, this GABA-lysine calcium supplement that promotes calcium absorption has the following beneficial effects: I. This invention forms a calcium source modified microsphere component by compounding seaweed calcium, dicalcium phosphate, chitosan, and phosphorylation reagent, which serves as the basic calcium source core material for the formulation. The component undergoes phosphorylation surface modification treatment to form a hydrophilic microsphere-level particle structure. At the same time, GABA, L-lysine, casein phosphopeptide, and carboxymethyl-β-cyclodextrin form a complex inclusion complex, which serves as the core absorption-promoting material for intestinal calcium absorption. The calcium source modified microsphere component and the absorption-promoting inclusion complex component form a functional fit, allowing the calcium source to exist in a stable structure while fully contacting the absorption-promoting components, thus constructing the basic conditions for intestinal calcium absorption.

[0018] II. This invention extracts effective components from natto and button mushrooms to form calcium-directed deposition components, which serve as functional materials for calcium-directed bone deposition. Natural vitamin E is used as a synergistic component to provide antioxidant protection for various active ingredients in the formulation. At the same time, gum arabic is added as an auxiliary component to allow the calcium-modified microsphere components, absorption-promoting inclusion components, calcium-directed deposition components, and synergistic components to be tightly combined to form a stable integrated composite microsphere structure. Each functional component relies on this structure to achieve physical adsorption and molecular binding, ensuring that the functions of each component are performed in an orderly manner, and realizing the full-process functional synergy of calcium from absorption to deposition.

[0019] Other advantages, objectives and features of the invention will be set forth in part in the description which follows, and in part will be apparent to those skilled in the art from the following examination or study, or may be learned from the practice of the invention. Attached Figure Description

[0020] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are merely some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without any creative effort.

[0021] Figure 1 A flowchart illustrating a method for preparing a GABA-lysine calcium supplement that promotes calcium absorption; Figure 2 A framework diagram of a method for preparing a GABA-lysine calcium supplement that promotes calcium absorption; Figure 3 This is a flowchart illustrating the preparation of calcium-source microspheres in a method for preparing a GABA-lysine calcium supplement that promotes calcium absorption. Detailed Implementation

[0022] To further illustrate the technical means and effects of the present invention in achieving its intended purpose, the following detailed description of the specific implementation methods, structures, features, and effects of the present invention, in conjunction with the accompanying drawings and preferred embodiments, is provided below.

[0023] Example 1: Raw material preparation (by weight): 79.9 parts of calcium-modified microspheres; 15 parts of the absorption-enhancing inclusion complex; Five portions of calcium-directed deposition fraction; 0.1 parts of synergistic component.

[0024] Raw material specifications: The calcium source modified microspheres consist of: seaweed calcium, dicalcium phosphate, chitosan, and phosphorylation reagent, all of which are industrial conventional food-grade raw materials with a purity of ≥98%. The seaweed calcium, dicalcium phosphate, chitosan, and phosphorylation reagent are compounded in a mass ratio of 80:20:10:3. Absorption-enhancing inclusion component: γ The aminobutyric acid (GABA), L-lysine, casein phosphopeptide (CPP), and carboxymethyl-β-cyclodextrin are all food additive grade raw materials with an active ingredient content of ≥99%. The molar ratio of GABA to L-lysine is 1:1.5, the casein phosphopeptide is 5% of the total mass of GABA and L-lysine, and the mass ratio of the mixture of GABA, L-lysine, and casein phosphopeptide to carboxymethyl-β-cyclodextrin is 1:2. Calcium-directed deposition components: Natto and button mushrooms are natural edible raw materials. The mass ratio of K2MK7 extracted from natto and button mushrooms to natural vitamin D2 is 1:20. Synergistic synergistic component: Natural vitamin E, purity ≥98%, added at 0.1% of the total mass of calcium source modified microsphere component, absorption-enhancing inclusion component, and calcium-directed deposition component; Auxiliary raw material: Gum arabic adhesive, as an adhesive excipient in the preparation of the formulation, is added at 2% of the total mass of the calcium-directed deposition component and the absorption-promoting inclusion component.

[0025] Preparation method: Algal calcium, dicalcium phosphate, chitosan, and phosphorylation reagent were mixed in a mass ratio of 80:20:10:3, and deionized water was added to prepare a homogeneous suspension. Microspheres were formed using an emulsification crosslinking method in an emulsification device to obtain basic calcium source microspheres. The formed basic calcium source microspheres were then placed in a phosphorylation reagent system for directional surface modification. During the modification process, the mixture was continuously stirred at 400 r / min for 1.5 h. After modification, the microspheres were washed with deionized water and dried at low temperature to obtain a hydrophilic calcium source modified microsphere component, such as... Figure 1 As shown.

[0026] Carboxymethyl-β-cyclodextrin was added to deionized water to prepare a saturated aqueous solution. Then, a mixture of GABA and L-lysine in a molar ratio of 1:1.5 was added, along with 5% (by mass) of casein phosphopeptide, where the mixture of GABA, L-lysine, and casein phosphopeptide was mixed with carboxymethyl-β-cyclodextrin at a mass ratio of 1:2. The mixture was stirred continuously at 300 rpm for 2.5 hours at a constant temperature of 45°C using the saturated aqueous solution method. After filtration and drying, the absorption-enhancing inclusion component was obtained, such as... Figure 2 As shown.

[0027] Natto and button mushrooms were pulverized separately, and K2MK7 and natural vitamin D2 were extracted separately using supercritical CO2 extraction at an extraction pressure of 35 MPa, an extraction temperature of 50 °C, and an extraction time of 2.5 h. The extracted K2MK7 and natural vitamin D2 were mixed at a mass ratio of 1:20 and purified by chromatography to obtain the calcium-directed deposition component.

[0028] The calcium-directed deposition component and the absorption-encapsulating component were mixed, and 2% of the total mass of the two components was added as a gum arabic binder. The mixture was stirred continuously at 500 r / min until the system was homogeneous and free of solid particles, thus obtaining an inclusion suspension.

[0029] The calcium-modified microspheres were immersed in an inclusion suspension and adsorbed by ultrasonic adsorption at 250W ultrasonic power for 30 minutes. Natural vitamin E was added to the system as a synergistic component. After thorough mixing, the mixture was dried by spray drying at an inlet temperature of 165℃ and an outlet temperature of 75℃ to obtain an integrated composite microsphere formulation.

[0030] Test results: The prepared integrated composite microsphere formulation was tested for multiple indicators: Particle size and uniformity: The overall microsphere size of the formulation is 60~90μm. The microspheres are regular in shape and uniform in size, with no adhesion or breakage. Structural integrity: After being treated in an artificial simulated gastric acid environment for 2 hours, the integrated composite microsphere formulation showed no structural damage or component loss. After being transferred into artificial simulated intestinal fluid, it could slowly swell, maintaining structural stability while achieving orderly release of components. Ingredient retention rate: The retention rates of the core active ingredients, GABA, L-lysine, K2MK7, and natural VD2, are all ≥98.5%, and the preparation process did not cause significant damage to the active ingredients; Intestinal fluid dispersibility: In artificial simulated intestinal fluid, it can be completely dispersed within 3 minutes, with no calcium source aggregation, and the contact area between the microspheres and the intestinal fluid meets the standard. Composition binding: After high-speed centrifugation at 8000r / min for 10min, there was no stratification or precipitation. The calcium source modified microspheres, absorption-promoting inclusion components, and calcium-directed deposition components were tightly bound in the system, and the integrated structure was stable. Preliminary stability at room temperature: After being sealed at room temperature for 30 days, the content of each core active ingredient did not decrease significantly, and the retention rate was ≥97%, with no oxidative deterioration.

[0031] Example 2: Raw material preparation (by weight): 74.9 parts of calcium-modified microspheres; 20 parts of the absorption-enhancing inclusion complex; Five portions of calcium-directed deposition fraction; 0.1 parts of synergistic component.

[0032] Raw material specifications: The calcium source modified microspheres consist of: seaweed calcium, dicalcium phosphate, chitosan, and phosphorylation reagent, all of which are industrial conventional food-grade raw materials with a purity of ≥98%. The seaweed calcium, dicalcium phosphate, chitosan, and phosphorylation reagent are compounded in a mass ratio of 80:20:10:3. Absorption-enhancing inclusion component: γ The aminobutyric acid (GABA), L-lysine, casein phosphopeptide (CPP), and carboxymethyl-β-cyclodextrin are all food additive grade raw materials with an active ingredient content of ≥99%. The molar ratio of GABA to L-lysine is 1:1.5, the casein phosphopeptide is 5% of the total mass of GABA and L-lysine, and the mass ratio of the mixture of GABA, L-lysine, and casein phosphopeptide to carboxymethyl-β-cyclodextrin is 1:2. Calcium-directed deposition components: Natto and button mushrooms are natural edible raw materials. The mass ratio of K2MK7 extracted from natto and button mushrooms to natural vitamin D2 is 1:20. Synergistic synergistic component: Natural vitamin E, purity ≥98%, added at 0.1% of the total mass of calcium source modified microsphere component, absorption-enhancing inclusion component, and calcium-directed deposition component; Auxiliary raw material: Gum arabic adhesive, as an adhesive excipient in the preparation of the formulation, is added at 2% of the total mass of the calcium-directed deposition component and the absorption-promoting inclusion component.

[0033] Preparation method: Algal calcium, dicalcium phosphate, chitosan, and phosphorylation reagent were mixed in a mass ratio of 80:20:10:3, and deionized water was added to prepare a homogeneous suspension. Microspheres were formed using an emulsification crosslinking method in an emulsification device to obtain basic calcium source microspheres. The formed basic calcium source microspheres were then placed in a phosphorylation reagent system for directional surface modification. During the modification process, the mixture was continuously stirred at 400 r / min for 1.5 h. After modification, the microspheres were washed with deionized water and dried at low temperature to obtain a hydrophilic calcium source modified microsphere component, such as... Figure 3 As shown.

[0034] Carboxymethyl-β-cyclodextrin was added to deionized water to prepare a saturated aqueous solution. Then, GABA and L-lysine were mixed in a molar ratio of 1:1.5, and casein phosphopeptide was added at 5% of the total mass of GABA and L-lysine. The mixture of GABA, L-lysine, and casein phosphopeptide was mixed with carboxymethyl-β-cyclodextrin in a mass ratio of 1:2. The mixture was stirred continuously at 300 r / min for 2.5 h at a constant temperature of 45 °C using the saturated aqueous solution method. After filtration and drying, the absorption-enhancing inclusion component was obtained.

[0035] Natto and button mushrooms were pulverized separately, and K2MK7 and natural vitamin D2 were extracted separately using supercritical CO2 extraction at an extraction pressure of 35 MPa, an extraction temperature of 50 °C, and an extraction time of 2.5 h. The extracted K2MK7 and natural vitamin D2 were mixed at a mass ratio of 1:20 and purified by chromatography to obtain the calcium-directed deposition component.

[0036] The calcium-directed deposition component and the absorption-encapsulating component were mixed, and 2% of the total mass of the two components was added as a gum arabic binder. The mixture was stirred continuously at 500 r / min until the system was homogeneous and free of solid particles, thus obtaining an inclusion suspension.

[0037] The calcium-modified microspheres were immersed in an inclusion suspension and adsorbed by ultrasonic adsorption at 250W ultrasonic power for 30 minutes. Natural vitamin E was added to the system as a synergistic component. After thorough mixing, the mixture was dried by spray drying at an inlet temperature of 165℃ and an outlet temperature of 75℃ to obtain an integrated composite microsphere formulation.

[0038] Formulation testing: The prepared integrated composite microsphere formulation was tested for multiple indicators: Particle size and uniformity: The overall microsphere size of the formulation is 55~85μm. The microspheres are regular and round, with uniform particle size, and there is no adhesion or breakage. Structural integrity: After being treated in an artificial simulated gastric acid environment for 2 hours, the integrated composite microsphere formulation showed no structural damage or component dissolution or shedding. After being transferred into artificial simulated intestinal fluid, the swelling rate was moderate, enabling the simultaneous and orderly release of absorption-promoting components and calcium sources. Ingredient retention rate: The retention rate of absorption-enhancing core active ingredients such as GABA and L-lysine is ≥99.0%, and the retention rate of K2MK7 and natural VD2 is ≥98.8%. The preparation process did not damage any of the active ingredients. Intestinal fluid dispersibility: In artificial simulated intestinal fluid, it can be completely and uniformly dispersed within 2 minutes without any calcium source agglomeration or clumping, maximizing the contact area between the microspheres and the intestinal fluid. Component binding: After high-speed centrifugation at 8000r / min for 10min, there was no stratification, no precipitation, and no free component phenomenon. Each functional component was tightly bound to the calcium source microspheres, and the integrated structure had excellent stability. Preliminary stability at room temperature: After the formulation was sealed and stored at room temperature for 30 days, the content of each core active ingredient did not fluctuate significantly, the retention rate was ≥97.5%, and there was no oxidative deterioration.

[0039] Comparative example: Raw material preparation (by weight): 79.9 parts of conventional calcium source components; 15 portions of standard absorption-enhancing ingredients; Five portions of calcium-directed deposition fraction; 0.1 parts of synergistic component.

[0040] Raw material specifications: Conventional calcium source components: calcium carbonate and dicalcium phosphate, both of which are industrial conventional food-grade calcium source raw materials with a purity of ≥98%. Calcium carbonate and dicalcium phosphate are mixed in a mass ratio of 7:3. No seaweed calcium is used, and there is no chitosan, phosphorylation reagent, or any microsphere molding or modification treatment. Conventional absorption-enhancing ingredients: casein phosphopeptide and vitamin C, both of which are food additive grade raw materials with an active ingredient content of ≥99%, directly compounded at a mass ratio of 4:1, without the use of GABA and L-lysine, and without carboxymethyl-β-cyclodextrin inclusion treatment; Calcium-directed deposition components: completely consistent with Example 1, natto and button mushrooms are natural edible raw materials, and the mass ratio of K2MK7 extracted from natto and button mushrooms to natural VD2 is 1:20. Synergistic synergistic component: completely consistent with Example 1, natural vitamin E, purity ≥98%, added at 0.1% of the total mass of conventional calcium source components, conventional absorption-promoting components, and calcium-directed deposition components; Auxiliary ingredient: Xanthan gum, added at 2% of the total mass of calcium-directed deposition components and conventional absorption-enhancing components.

[0041] Preparation method: Calcium carbonate and calcium hydrogen phosphate were directly mixed at a mass ratio of 7:3, and deionized water was added to prepare a suspension. After low-temperature drying, the conventional calcium source component was obtained.

[0042] Casein phosphopeptide and vitamin C were added to deionized water at a mass ratio of 4:1, stirred at 300 r / min for 30 min at room temperature, and then dried directly to obtain the conventional absorption-enhancing component.

[0043] The preparation method of the calcium-directed deposition component is exactly the same as in Example 1. Natto and button mushrooms are crushed separately, and K2MK7 and natural VD2 are extracted separately by supercritical CO2 extraction. The extraction pressure is 35 MPa, the extraction temperature is 50℃, and the extraction time is 2.5 h. The extracted K2MK7 and natural VD2 are mixed at a mass ratio of 1:20 and purified by chromatography to obtain the calcium-directed deposition component.

[0044] The calcium-directed deposition component was mixed with a conventional absorption-enhancing component, and xanthan gum, accounting for 2% of the total mass of the two components, was added. The mixture was stirred continuously at 500 r / min until the system was homogeneous, resulting in a conventional mixed suspension.

[0045] The conventional calcium source components were added to a conventional mixed suspension and stirred at room temperature. Natural vitamin E was added to the system as a synergistic component. After thorough mixing, the mixture was dried by spray drying at an inlet temperature of 165°C and an outlet temperature of 75°C to obtain a conventional compound calcium supplement.

[0046] Test results: The prepared conventional compound calcium supplement was tested for the same indicators as in Examples 1 and 2: Particle size and uniformity: It is an irregular particulate mixture with a particle size distribution of 30~120μm. The particle size uniformity is poor, and there is slight adhesion and partial agglomeration. Structural integrity: After being treated in an artificial simulated gastric acid environment for 2 hours, the structural integrity was poor, with a small amount of components dissolving and being lost. After being transferred to artificial simulated intestinal fluid, the swelling was irregular, the release rate was too fast, and there was no orderly release effect. Ingredient retention rate: The effective activity retention rate of conventional absorption-enhancing ingredients is 82.3%, while the retention rate of K2MK7 and natural VD2 is 86.7% due to the lack of effective protection measures. Gastric acid treatment and preparation process cause a certain degree of damage to active ingredients. Intestinal fluid dispersibility: In artificial simulated intestinal fluid, it failed to disperse completely within 8 minutes, and the calcium source showed obvious aggregation. The contact area with the intestinal fluid was significantly lower than that in Example 1 and Example 2. Insufficient contact area affected the subsequent absorption efficiency. Component binding: After high-speed centrifugation at 8000r / min for 10min, slight stratification and a small amount of precipitation occurred, indicating that the components were not tightly bound and the system structure was generally unstable. Preliminary stability at room temperature: After being sealed at room temperature for 30 days, the content of each core active ingredient decreased significantly, with an overall retention rate of 78.5%, showing slight oxidation and deterioration, and slow degradation of some components.

[0047] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present invention. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A GABA-lysine calcium supplement that promotes calcium absorption, characterized in that, This formulation, by weight, comprises: Calcium-modified microspheres: 60-80 parts; Absorption-enhancing inclusion complex: 15-25 parts; Calcium-directed deposition components: 3-8 parts; Synergistic effect component: 0.05~0.15 parts.

2. The GABA-lysine calcium supplement that promotes calcium absorption according to claim 1, characterized in that, The calcium source modified microsphere component is a compound of seaweed calcium, dicalcium phosphate, chitosan, and phosphorylation reagent. The mass ratio of seaweed calcium, dicalcium phosphate, chitosan, and phosphorylation reagent in the calcium source modified microsphere component is 80:18~22:9~11:2.8~3.

2. The calcium source modified microsphere component is a hydrophilic composite calcium source microsphere obtained by phosphorylation surface modification and has a microsphere-level particle structure.

3. The GABA-lysine calcium supplement that promotes calcium absorption according to claim 1, characterized in that, The absorption-enhancing inclusion component is a complex inclusion compound of GABA, L-lysine, casein phosphopeptide, and carboxymethyl-β-cyclodextrin, with an inclusion rate ≥95%. The molar ratio of GABA to L-lysine is 1:1.5, the amount of casein phosphopeptide added is 4.8~5.2% of the total mass of GABA and L-lysine, and the mass ratio of GABA, L-lysine, casein phosphopeptide, and carboxymethyl-β-cyclodextrin is 0.8~1.2:1.8~2.

2.

4. The GABA-lysine calcium supplement that promotes calcium absorption according to claim 1, characterized in that, The calcium-directed deposition component is a composition of K2MK7 and natural VD2, which has a homogeneous fat-soluble liquid structure. K2MK7 and natural VD2 are extracted from natto and button mushrooms, respectively, and the mass ratio of K2MK7 to natural VD2 is 0.8~1.2:18~22.

5. A GABA-lysine calcium supplement that promotes calcium absorption according to claim 1, characterized in that, The synergistic enhancing component is natural vitamin E, and the amount added is 0.08~0.12% of the total mass of the calcium source modified microsphere component, the absorption-promoting inclusion component, and the calcium-directed deposition component.

6. A GABA-lysine calcium supplement that promotes calcium absorption according to claim 1, characterized in that, The formulation is prepared by forming an integrated composite microsphere structure through calcium source modified microsphere components, absorption-promoting inclusion components, calcium-directed deposition components, and synergistic components. During the preparation of the formulation, gum arabic binder is added in an additional amount of 1.8 to 2.2% of the total mass of calcium-directed deposition components and absorption-promoting inclusion components.

7. A GABA-lysine calcium supplement that promotes calcium absorption according to claim 1, characterized in that, The preparation method of this formulation is as follows: Preparation of calcium source microspheres: Algal calcium, dicalcium phosphate, chitosan and phosphorylation reagent are mixed and prepared by emulsification crosslinking method to obtain basic calcium source microspheres. The basic calcium source microspheres are then subjected to surface phosphorylation modification treatment to obtain calcium source modified microsphere components. Preparation of complex inclusion complex: GABA, L-lysine, casein phosphopeptide and carboxymethyl-β-cyclodextrin were mixed and formed into an inclusion structure by saturated aqueous solution method to obtain an absorption-enhancing inclusion component; Preparation of depositional components: K2MK7 was extracted from natto and natural VD2 was extracted from button mushrooms. K2MK7 and natural VD2 were mixed and purified to obtain calcium-directed depositional components. Preparation of inclusion suspension: The calcium-directed deposition component and the absorption-promoting inclusion component are mixed, gum arabic binder is added and the mixture is stirred continuously to form a homogeneous inclusion suspension without particles; Preparation of composite microspheres: The calcium-modified microsphere components are immersed in an inclusion suspension for adsorption treatment, and synergistic components are added and stirred to mix evenly. The integrated composite microsphere formulation is then obtained through a drying process.

8. A GABA-lysine calcium supplement that promotes calcium absorption according to claim 7, characterized in that, In the preparation of calcium source microspheres, seaweed calcium, dicalcium phosphate, chitosan, and phosphorylation reagent are mixed and then added to deionized water to form a homogeneous suspension. Microspheres are formed in an emulsification device using an emulsification crosslinking method to obtain basic calcium source microspheres with a particle size of 50-80 μm. The surface phosphorylation modification treatment involves placing the formed basic calcium source microspheres in a phosphorylation reagent system for directional surface modification. During the modification process, the microspheres are continuously stirred at a speed of 300-500 r / min for 1-2 h. After modification, the microspheres are washed with deionized water and dried at low temperature to obtain a hydrophilic calcium source modified microsphere component.

9. A GABA-lysine calcium supplement that promotes calcium absorption according to claim 7, characterized in that, In the preparation of the composite inclusion complex, carboxymethyl-β-cyclodextrin is added to deionized water to prepare a saturated aqueous solution, and then GABA, L-lysine and casein phosphopeptide are added. The mixture is stirred continuously at 200-400 r / min for 2-3 hours in a constant temperature environment of 40-50℃ using the saturated aqueous solution method. After filtration and drying, an absorption-enhancing inclusion component with an inclusion rate of ≥95% is obtained.

10. A GABA-lysine calcium supplement that promotes calcium absorption according to claim 7, characterized in that, In the preparation of the deposition component, natto and button mushrooms are crushed separately, and K2MK7 and natural VD2 are extracted separately by supercritical CO2 extraction. The extraction pressure is 30~40MPa, the extraction temperature is 45~55℃, and the extraction time is 2~3h. Then, K2MK7 and natural VD2 are mixed and purified by chromatography to obtain a calcium-oriented deposition component with a purity ≥98%.

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