Akk bacteria composition with plant extract for regulating blood sugar

Abstract

The present application provides a kind of Akk bacteria composition containing plant extract for regulating blood sugar, the composition has the effects of regulating sugar metabolism and lipid metabolism, regulating blood sugar, improving brain fog, improving insulin sensitivity, regulating blood lipids, controlling weight, reducing fat accumulation, inhibiting obesity and the like.

Description

[0001] This application claims priority to Chinese Application No. 202510193485.9, filed on February 21, 2025, entitled “An Akk bacteria composition containing plant extracts for regulating blood sugar”, the entire contents of which are incorporated herein by reference. Technical Field

[0002] This invention belongs to the field of health products and relates to an Akk bacteria composition containing plant extracts for regulating blood sugar. It has the effects of regulating sugar and lipid metabolism, regulating blood sugar, improving brain fog, improving insulin sensitivity, regulating blood lipids, controlling weight, reducing fat accumulation, and inhibiting obesity. Background Technology

[0003] Glucose and lipid metabolism is a crucial metabolic pathway for energy conversion in the body. Abnormalities in glucose and lipid metabolism can lead to metabolic diseases such as diabetes, obesity, anorexia, and fatty liver. Long-term metabolic disorders can cause numerous complications, including cardiovascular and cerebrovascular diseases, peripheral neuropathy, retinopathy, nephropathy, and diabetes. Hyperglycemia refers to a blood sugar level higher than normal; it is a metabolic disease primarily caused by impaired insulin action, insulin secretion defects, or both. Hyperglycemia is a chronic disease, and its long-term development can severely damage various organs and blood vessels, seriously endangering human health.

[0004] Studies have shown that gut microbiota is highly correlated with chronic diseases such as obesity and diabetes in the host. Akkermansia has the effect of lowering blood sugar levels and improving host glucose tolerance. It can improve host glucose tolerance and lower host blood sugar levels by negatively regulating IFN-γ.

[0005] The plant extracts have the effect of helping to lower blood sugar. They can promote liver glycogen synthesis by regulating sugar metabolism, stimulate insulin secretion, improve insulin resistance, inhibit α-glucosidase activity and glucose transport, and improve the level of oxidative stress in the body.

[0006] Combining Akk bacteria with plant extracts has a synergistic effect, and can regulate sugar and lipid metabolism, regulate blood sugar, improve brain fog, improve insulin sensitivity, regulate blood lipids, control weight, reduce fat accumulation, and inhibit obesity. Summary of the Invention

[0007] The purpose of this invention is to provide an Akk bacteria composition containing plant extracts for regulating blood sugar, which has the effects of regulating sugar and lipid metabolism, regulating blood sugar, improving brain fog, improving insulin sensitivity, regulating blood lipids, controlling weight, reducing fat accumulation, and inhibiting obesity.

[0008] The compositions of the present invention have the following advantages:

[0009] 1. It can increase the body's basal metabolic rate and regulate glucose and lipid metabolism, with significant effects and no side effects;

[0010] 2. Akk can colonize the intestines, effectively improve the intestinal environment, enhance intestinal barrier function, alleviate oxidative stress, and repair pancreatic function;

[0011] 3. It can regulate blood sugar and improve insulin sensitivity, especially for middle-aged and elderly people, and can effectively improve abnormal blood lipids and blood sugar in middle-aged and elderly people;

[0012] 4. It can control weight, reduce fat accumulation, and inhibit obesity, especially for people who sit for long periods of time, as it can reduce the accumulation of body fat.

[0013] The present invention provides an Akk bacteria composition containing plant extracts for regulating blood sugar, wherein the Akk bacteria includes live Akk bacteria and / or Akk postbiotics.

[0014] In one embodiment, the composition of the present invention wherein the Akk postbiotic is an inactivated bacterium, a lysed bacterium, and / or a culture medium containing dead Akk cells.

[0015] In one embodiment, the Akk postbiotic in the composition of the present invention is inactivated by pasteurization, heating, ultraviolet irradiation, or chemical treatment, preferably by pasteurization.

[0016] In one embodiment, the composition of the present invention comprises the following plant extracts: bitter melon extract, mulberry leaf extract, coptis extract, gynostemma pentaphyllum extract, highland barley extract, potato extract, sugarcane extract, green tea extract, fenugreek extract, ivy extract, cactus extract, ginseng extract, cinnamon extract, garlic extract, grapefruit extract, monk fruit extract, raspberry extract, red ginseng extract, Solomon's seal extract, chia seed extract, yam extract, kudzu root extract, ophiopogon japonicus extract, garcinia cambogia extract, amla extract, grape seed extract, cranberry extract, and white... One or more of the following extracts: kidney bean extract, red beet extract, black ginger extract, five-layered dragon extract, eucommia leaf extract, onion extract, wood ear fungus extract, okra extract, Jerusalem artichoke root extract, moth leaf extract, crape myrtle leaf extract, gymnospermum extract, blueberry extract, Chinese tallow tree root extract, centella asiatica leaf extract, panna cochinchinensis extract, holy basil leaf extract, clove extract, myrica rubra extract, eucalyptus extract, tartary buckwheat extract, purslane extract, Agaricus blazei extract, dandelion extract, cherry extract, sea buckthorn oil extract, American ginseng extract, corn silk extract, and mulberry extract.

[0017] In one embodiment, the active ingredients of the plant extract in the composition of the present invention are one or more of the following: momordicin, momordicin, alkaloids, carotene, bitter principles, momordicin saponins, momordic polypeptides, glucosides, mulberry polysaccharides, gypsum acid, anthocyanins, ginkgo flavonoids, terpene lactones, corosolic acid, flavonoids, berberine, quercetin, ursolic acid, oleanolic acid, myricetin, organic acids, volatile oils, polysaccharides, terpenoids, flavonoids, phenolic compounds, alkaloids, glycosides, sterols, minerals, quinones, lignans, coumarins, saponins, cardiac glycosides, phenolic acids, amino acids, 1-deoxynojirimycin (DNJ), puerarin, and alkaloids.

[0018] In one embodiment, the composition of the present invention contains a plant extract at a concentration ≥1 mg / part. Examples include 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, and 1000 mg / part.

[0019] In one application method, the composition of the present invention contains ≥10 mg / part of plant extract.

[0020] In one application method, the composition of the present invention contains ≥20 mg / part of the plant extract.

[0021] In one application method, the composition of the present invention contains ≥50 mg / part of plant extract.

[0022] In one embodiment, the composition of the present invention contains a plant extract at a concentration ≥100 mg / part, such as 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, or 1000 mg / part.

[0023] In a preferred embodiment, the composition of the present invention contains 400-1500 mg / part of plant extract.

[0024] In a preferred embodiment, the composition of the present invention comprises at least one or more of bitter melon extract, mulberry extract, and mulberry leaf extract.

[0025] In a more preferred embodiment, the composition of the present invention comprises at least one or more of bitter melon extract and mulberry leaf extract.

[0026] In one embodiment, the active ingredients of the bitter melon extract in the composition of the present invention are one or more of the following: momordicin, momordicin, alkaloids, carotene, bitter principles, momordic saponins, momordic polypeptides, and glucosides.

[0027] In one embodiment, the composition of the present invention contains bitter melon extract at a content of ≥1 mg / part.

[0028] In one embodiment, the composition of the present invention contains bitter melon extract at a content of ≥10 mg / part.

[0029] In one embodiment, the composition of the present invention contains bitter melon extract at a content of ≥20 mg / part.

[0030] In one embodiment, the composition of the present invention contains bitter melon extract at a concentration of ≥50 mg / part, such as 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, or 1000 mg / part.

[0031] In a preferred embodiment, the composition of the present invention contains 100-800 mg / part of bitter melon extract.

[0032] In a more preferred embodiment, the composition of the present invention contains 300-800 mg / part of bitter melon extract.

[0033] In a particularly preferred embodiment, the composition of the present invention contains 400-700 mg of bitter melon extract per serving.

[0034] In one embodiment, the composition of the present invention contains bitter melon extract with a content of ≥7% bitter quinolone.

[0035] In one embodiment, the active ingredient of the mulberry leaf extract in the composition of the present invention is one or more of 1-deoxynojirimycin (DNJ), mulberry leaf polysaccharide, mulberry leaf flavonoids, alkaloids, and mulberry leaf polyphenols.

[0036] In one embodiment, the active ingredient of the mulberry leaf extract in the composition of the present invention is one or more of 1-deoxynojirimycin (DNJ), mulberry leaf polysaccharide, mulberry leaf flavonoids, and alkaloids.

[0037] In one embodiment, the composition of the present invention contains mulberry leaf extract at a content of ≥1 mg / part.

[0038] In one embodiment, the composition of the present invention contains mulberry leaf extract at a content of ≥10 mg / part.

[0039] In one embodiment, the composition of the present invention contains ≥20 mg / part of mulberry leaf extract.

[0040] In one embodiment, the composition of the present invention contains mulberry leaf extract at a concentration of ≥50 mg / part, such as 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, or 1000 mg / part.

[0041] In a preferred embodiment, the composition of the present invention contains 100-800 mg / part of mulberry leaf extract.

[0042] In a more preferred embodiment, the composition of the present invention contains 500-800 mg / part of mulberry leaf extract.

[0043] In one embodiment, the active ingredients of the composition of the present invention, namely the kudzu root extract, are one or more of puerarin, flavonoids, coumarins, and amino acids.

[0044] In one embodiment, the composition of the present invention contains ≥1 mg / part of kudzu root extract.

[0045] In one embodiment, the composition of the present invention contains ≥10 mg / part of kudzu root extract.

[0046] In one embodiment, the composition of the present invention contains ≥20 mg / part of kudzu root extract.

[0047] In one embodiment, the active ingredients of the plant extract in the composition of the present invention include at least one or more of the following: momordicin, momordicin, alkaloids, carotene, bitter principles, momordic saponins, momordic polypeptides, glucosides, gypsum acid, anthocyanins, ginkgo flavonoids, terpene lactones, corosolic acid, flavonoids, berberine, quercetin, lignans, saponins, 1-deoxynojirimycin (DNJ), mulberry leaf polysaccharides, mulberry leaf flavonoids, mulberry leaf polyphenols, and alkaloids.

[0048] In one embodiment, the composition of the present invention contains berberine at a content of ≥1 mg / part.

[0049] In one embodiment, the composition of the present invention contains berberine at a content of ≥5 mg / part.

[0050] In one embodiment, the composition of the present invention contains berberine at a content of ≥10 mg / part.

[0051] In one embodiment, the composition of the present invention contains berberine at a content of ≥50 mg / part, such as 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, or 1000 mg / part.

[0052] In a preferred embodiment, the composition of the present invention contains 50-2000 mg / part of berberine.

[0053] In a more preferred embodiment, the composition of the present invention contains 100-1500 mg / part of berberine.

[0054] In a particularly preferred embodiment, the composition of the present invention contains 500-1500 mg / part of berberine.

[0055] In one embodiment, the composition of the present invention has an Akk bacterial cell count ≥ 1 × 10⁻⁶. 7 cfu / serving.

[0056] In one embodiment, the composition of the present invention has an Akk bacterial cell count ≥ 1 × 10⁻⁶. 8 cfu / serving.

[0057] In one embodiment, the composition of the present invention has an Akk bacterial cell count ≥ 1 × 10⁻⁶. 9 cfu / serving.

[0058] In one embodiment, the composition of the present invention has an Akk bacterial cell count ≥ 1 × 10⁻⁶. 10 cfu / serving.

[0059] In one embodiment, the composition of the present invention further includes other probiotics beneficial to the gut, said other gut probiotics being one or more of the genera *Lactobacillus*, *Bifidobacterium*, *Lactobacillus*, *Lactobacillus*, *Enterococcus*, *Bacillus*, *Lactobacillus mucosae*, *Streptococcus*, and *Lactococcus*.

[0060] In one embodiment, the composition of the present invention includes, among other intestinal probiotics, *Lactobacillus paracasei*, *Bifidobacterium animalis* subsp. *lactobacter*, *Lactobacillus acidophilus*, *Lactobacillus plantarum*, *Bifidobacterium longum* subsp. *longum*, *Bifidobacterium longum* subsp. *infanthi*, *Lactobacillus rhamnosus*, *Enterococcus faecalis*, *Lactobacillus casei*, *Bifidobacterium adolescentis*, *Bifidobacterium animalis* subsp. *animal*, *Bifidobacterium bifidum*, *Bifidobacterium pseudosporidis*, *Bacillus subtilis ...Mucobacterium reuteri*, and *Mucobacterium fermentum*. The bacteria include one or more of the following: *Streptococcus salivarius* subsp. *thermophilus*, *Lactobacillus helveticus*, *Lactobacillus brevis*, *Lactobacillus delbrueckii* subsp. *bulgaricus*, *Lactobacillus delbrueckii* subsp. *lactobacter*, lactic acid bacteria, *Lactococcus lactis*, *Lactobacillus gasseri*, *Lactobacillus johnsonii*, *Lactobacillus maltii* subsp. *maltii*, *Pediococcus pentosus*, *Pediococcus lactis*, *Streptococcus salivarius* subsp. *thermophilus*, *Enterococcus faecalis*, *Bacillus coagulans*, *Bacillus licheniformis*, *Bacillus natto*, *Clostridium butyricum*, *Vibrio butyricum*, and lactic acid bacteria.

[0061] In one embodiment, the composition of the present invention contains other intestinal probiotics with a cell count ≥1×10⁻⁶. 5 CFU / part composition.

[0062] In a preferred embodiment, the composition of the present invention contains other intestinal probiotics with a cell count ≥1×10⁻⁶. 7 CFU / part composition.

[0063] In one embodiment, the composition of the present invention further includes trace elements, and more specifically, the trace elements are one or more of chromium, zinc, magnesium, and manganese.

[0064] In a preferred embodiment, the chromium in the composition of the present invention is one or more of chromium pyridinecarboxylate and chromium polyhydrochloride.

[0065] In a preferred embodiment, the magnesium in the composition of the present invention is one or more of magnesium oxide, magnesium citrate, magnesium carbonate, magnesium sulfate, magnesium chloride, magnesium threonate, and magnesium gluconate.

[0066] In one embodiment, the composition of the present invention contains ≥1 mg / part of chromium.

[0067] In a preferred embodiment, the composition of the present invention contains ≥5 mg / part of chromium.

[0068] In a more preferred embodiment, the composition of the present invention contains ≥10 mg / part of chromium.

[0069] In a particularly preferred embodiment, the composition of the present invention contains ≥50 mg / part of chromium. For example, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 350, or 400 mg / part.

[0070] In a particularly preferred embodiment, the composition of the present invention contains 50-300 mg / part of chromium.

[0071] In one embodiment, the composition of the present invention further includes fungi.

[0072] In one embodiment, the composition of the present invention comprises one or more of Chaga mushroom, Grifola frondosa, Hericium erinaceus, and Cordyceps sinensis.

[0073] In one embodiment, the composition of the present invention contains, but is not limited to, polysaccharides and triterpenoids as active ingredients of the fungus; further, the polysaccharides include, but are not limited to, fungal polysaccharides and plant fiber polysaccharides.

[0074] In one embodiment, the composition of the present invention further includes vitamins, and more specifically, the vitamins include one or more of vitamin B, vitamin C, vitamin E, and vitamin D.

[0075] In one embodiment, the composition of the present invention further includes dietary fiber, and the dietary fiber includes, but is not limited to, pectin, oligosaccharides, cellulose, lignin, and hemicellulose.

[0076] In one embodiment, the composition of the present invention further includes sugar alcohols, and further, the sugar alcohols include, but are not limited to, sorbitol, xylitol, mannitol, maltitol, and lactitol.

[0077] In one embodiment, the method for preparing the plant extract is selected from enzymatic hydrolysis, water extraction, alcohol extraction, ultrasonic extraction, and supercritical fluid extraction.

[0078] In one embodiment, the method for preparing the plant extract specifically includes: (1) selecting and processing plant raw materials, such as washing, cutting or crushing;

[0079] (2) Active ingredients are extracted using enzymatic hydrolysis, water extraction, alcohol extraction, ultrasonic extraction, and supercritical fluid extraction.

[0080] (3) The extract was separated and purified by methods such as filtration, centrifugation, chromatography, membrane separation and crystallization;

[0081] (4) Concentrate and dry the extract using techniques such as evaporation, freeze drying or spray drying.

[0082] This invention provides an Akk bacteria composition containing plant extracts for regulating blood sugar. The composition is prepared into a human-acceptable formulation by adding excipients, including but not limited to tablets, soft capsules, capsules, powders, oral liquids, sprays, solid beverages, gummies, jellies, and crystals.

[0083] The present invention also provides the use of any of the Akk bacteria compositions containing plant extracts in regulating glucose and lipid metabolism, regulating blood sugar, improving brain fog, improving insulin sensitivity, regulating blood lipids, controlling weight, reducing fat accumulation, and inhibiting obesity.

[0084] Furthermore, the present invention also provides the use of any of the Akk bacteria compositions containing plant extracts in regulating blood sugar, improving insulin sensitivity, and regulating blood lipids.

[0085] Furthermore, the present invention provides the use of any of the Akk bacteria compositions containing plant extracts in regulating sugar and lipid metabolism.

[0086] Furthermore, the present invention provides the use of any of the Akk bacteria compositions containing plant extracts in controlling weight, reducing fat accumulation, and inhibiting obesity.

[0087] Terminology Explanation

[0088] Akkermansia muciniphila (Akk) is a common intestinal bacterium, a Gram-negative bacterium belonging to the phylum Verrucous. This bacterium primarily inhabits the intestines of humans and animals and can break down mucin in the intestinal mucus layer. It is a member of the gut microbiota and plays a role in enhancing the intestinal barrier. Numerous studies have shown that the occurrence, development, and outcome of many clinical diseases (such as obesity, diabetes, pancreatitis, fatty liver, inflammatory bowel disease, and cancer) are closely related to abnormal intestinal barrier function. Intervention and treatment targeting abnormal intestinal barrier function have a very positive effect on preventing disease progression and accelerating recovery.

[0089] The term "postbiotics" refers to the collective components of probiotics after processing, including bacterial cells and metabolites. As inanimate microorganisms, postbiotics mainly consist of inactivated bacterial cells, bacterial lysates, cell wall components, and metabolites.

[0090] The "AKK inactivated bacteria" are AKK bacteria that have lost their ability to reproduce but retain their structural and / or functional components through physical or chemical treatment methods (such as pasteurization, radiation, etc.).

[0091] The "Akk lysate" is produced by mechanically or chemically disrupting the cell wall of AKK bacteria, releasing intracellular components, including but not limited to proteins, lipids, and nucleic acids.

[0092] The term "pasteurization" refers to a mild sterilization method that kills or inactivates microorganisms by heating to a certain temperature (usually not exceeding 100°C) and maintaining it for a period of time, while minimizing damage to the product's components.

[0093] The term "Akk pasteurized bacteria" refers to Akkella myxophilus (Akk bacteria) that has undergone pasteurization and is no longer viable. Pasteurized Akkella myxophilus is a metabiotic; although it loses its ability to grow after inactivation, its cellular components (such as cell walls and cell membranes) and metabolites may still have beneficial effects on the host, such as improving gut health and enhancing immune function.

[0094] The term "probiotics" refers to a general term for live beneficial microorganisms that can improve the composition and microecological balance of a host's flora in a certain part of the body and exert beneficial effects by colonizing the human body (mainly the intestines and reproductive system).

[0095] The term "plant extract" refers to a substance extracted or processed from plants (whole or part of a plant) using appropriate solvents or methods. Attached Figure Description

[0096] Figure 1 Results of blood glucose level detection in a zebrafish type II diabetes model;

[0097] Figure 2 The results show the blood glucose levels in a zebrafish type 1 diabetes model. Detailed Implementation

[0098] To facilitate understanding by those skilled in the art, the present invention will be further described below with reference to embodiments. The content mentioned in the embodiments is not intended to limit the present invention.

[0099] Example

[0100] Example 1

[0101] The composition was obtained by adding bitter melon extract and Akkermansia muciniphila (Akk bacteria).

[0102] Example 2

[0103] A composition was obtained by adding bitter melon extract, Lactobacillus paracasei, and Akkermansia muciniphila (Akk bacteria).

[0104] Example 3

[0105] A composition was obtained by adding bitter melon extract, Bifidobacterium lactis subsp. animalis, Gynostemma pentaphyllum extract, and Akkermansia muciniphila (Akk bacteria).

[0106] Example 4

[0107] The composition was obtained by adding bitter melon extract and Akk pasteurization bacteria (Akk pasteurization bacteria).

[0108] Example 5

[0109] A composition was obtained by adding bitter melon extract, Akkella myxophilus (Akk bacteria), and Akkella myxophilus pasteurized bacteria (Akk pasteurized bacteria).

[0110] Example 6

[0111] A composition was obtained by adding bitter melon extract, Akkella myxophilus, Akkella pasteurization, and green tea extract.

[0112] Example 7

[0113] A composition was obtained by adding bitter melon extract, Akkella myxophilus, Akkella pasteurization, green tea extract, and Lactobacillus plantarum.

[0114] Example 8

[0115] A composition was obtained by adding mulberry leaf extract, Akkella mycotoxinophilus, Akkella pasteurization, and Lactobacillus plantarum.

[0116] Example 9

[0117] A composition was obtained by adding mulberry extract, Akk Pasteurella multocida (Akk Pasteurella multocida), and Lactobacillus rhamnosus.

[0118] Example 10

[0119] The composition was obtained by adding mulberry extract, bitter melon extract, Akk Pasteurella multocida (Akk Pasteurella multocida), Lactobacillus rhamnosus, and Barnabas extract.

[0120] Example 11

[0121] A composition was obtained by adding bitter melon extract, Akkella mycotoxinophilus, Akkella pasteurization, red ginseng extract, Lactobacillus plantarum, and Bifidobacterium animalis subsp. lactis.

[0122] Example 12

[0123] The composition was obtained by adding mulberry leaf extract, bitter melon extract, Akk pasteurization bacteria, Lactobacillus rhamnosus, and Barnabas extract.

[0124] Example 13

[0125] A composition was obtained by adding mulberry leaf extract, bitter melon extract, Akk pasteurization bacteria (Akk pasteurization bacteria), Lactobacillus rhamnosus, and Gynostemma pentaphyllum extract.

[0126] Example 14

[0127] The composition was obtained by adding mulberry leaf extract, bitter melon extract, Akk pasteurizer (Akk pasteurizer), and green tea extract.

[0128] Example 15

[0129] A composition was obtained by adding mulberry leaf extract, Akkella myxophilus, Akkella pasteurization, red ginseng extract, and Lactobacillus plantarum.

[0130] Example 16

[0131] 600 mg of bitter melon extract was mixed with 1×10 8 CFU probiotics (Akk bacteria, Akk pasteurized bacteria) are mixed and added to a jelly base (water, sugar, carrageenan, etc.) to produce Akk bacteria jelly containing bitter melon extract. The preparation method includes mixing bitter melon extract with Akk bacteria solution, adding the jelly base and stirring evenly, heating to boiling, filling into aseptic jelly strips, cooling and solidifying, and then sealing.

[0132] Example 17

[0133] 600 mg of bitter melon extract was mixed with 1×10 8CFU probiotics (Akk bacteria, Akk pasteurized bacteria) are mixed, and then excipients such as rice flour and maltodextrin are added to produce Akk bacteria powder strips containing bitter melon extract. The preparation method includes mixing bitter melon extract with other excipients, mixing it evenly with bacterial solution, spray drying it into powder, and then filling it into aseptic strips.

[0134] Example 18

[0135] 750 mg of mulberry leaf extract was mixed with 1×10 8 CFU probiotics (Akk bacteria, Akk pasteurized bacteria) are mixed, and appropriate amounts of glycerol, potassium sorbate, and other stabilizers and preservatives are added to produce an Akk bacteria oral liquid containing mulberry leaf extract. The preparation method includes dissolving the mulberry leaf extract in sterile water, mixing it evenly with the Akk bacteria, adjusting the pH value, filtering to remove bacteria, filling into sterile bottles, and sealing.

[0136] Example 19

[0137] 600 mg of bitter melon extract was mixed with 1×10 8 CFU probiotics (Akk bacteria, Akk pasteurized bacteria) are mixed and appropriate amounts of syrup, gelatin, pectin, and other excipients are added to produce Akk bacteria gummies containing bitter melon extract. The preparation method involves thoroughly mixing bitter melon extract and Akk bacteria, adding excipients, cooking sugar to obtain a thick syrup, cooling to a suitable temperature, and cutting to obtain Akk bacteria gummies containing plant extracts.

[0138] Example 20

[0139] 800 mg of Coptis chinensis extract was mixed with 1×10 8 CFU probiotics (Akk bacteria, Akk pasteurized bacteria) are mixed with appropriate amounts of calcium bicarbonate, pregelatinized starch, and other excipients to produce Akk bacteria tablets containing bitter melon extract. The preparation method includes unpacking, cleaning, sterilizing, and sieving the excipients, mixing them with bitter melon extract and Akk bacteria, adding a lubricant, inspecting the tableting process, and drying to obtain Akk bacteria tablets containing plant extracts.

[0140] Biological Experiment Examples

[0141] Biological Experiment Example 1: Evaluation of Blood Glucose Lowering Efficacy

[0142] 1.1 Laboratory Animals

[0143] Zebrafish were all raised in aquarium water at 28℃ (water quality: 200mg of readily soluble sea salt added per 1L of reverse osmosis water; conductivity 450~550μS / cm; pH 6.5~8.5; hardness 50~100mg / L CaCO3), bred and provided by our company's aquarium. Wild-type AB strain zebrafish were bred through natural pair mating. Zebrafish aged 5 days post-fertilization (5dpf) were used to evaluate their hypoglycemic efficacy.

[0144] 1.2 Experimental Instruments and Reagents

[0145] Dissecting microscope (Mingmei, China); Precision electronic balance (XSR205, METTLER, USA); Alloxan: batch number G102420, Aladdin, concentration 0.025mM; Glucose: batch number G123464, Aladdin, prepared with standard water, concentration 18.0mg / mL.

[0146] 1.3 Experimental Methods and Results

[0147] Wild-type AB strain zebrafish (5 dpf) were randomly selected and placed in 50 mL beakers, with 50 fish / 30 mL standard dilution water / beaker for volume adjustment. Water-soluble samples (Example 1) were prepared. A normal control group and a model control group were also set up. Except for the normal control group, the other experimental groups were given 18 mg / mL glucose or 18 mg / mL glucose + 0.025 mM alloxan to establish type II and type I diabetes models, respectively. At 15:00, according to the groups, different concentrations of sample were added to each well (groups and concentrations are shown in Table 1), thoroughly mixed, wrapped in aluminum foil, labeled, and incubated at 28℃ for 24 h. At 15:00 the next day, all zebrafish from each experimental group were collected, ground, centrifuged, and the supernatant was used to determine the blood glucose concentration of the zebrafish according to the kit instructions. Statistical analysis was performed to evaluate the hypoglycemic efficacy of the samples. Statistical results are expressed as Mean ± SEM. Graphadprism analysis was used for statistical analysis; p < 0.05 indicated statistical significance.

[0148] Table 1

[0149] Grouping Dosage concentration normal control group / Model Group 1 18 mg / mL glucose solution (GLU) Positive group 1 <![CDATA[18 mg / mL Glucose Solution (GLU) + Example 1 (400 μg / mL Momordica charantia Extract + 1×10 6 CFU / mL Akkermansia muciniphila)]]> Model Group 2 18 mg / mL glucose solution (GLU) + 0.025 mM alloxan (ALX) Positive group 2 <![CDATA[18 mg / mL glucose solution (GLU) + 0.025 mM alloxan (ALX) + Example 1 (400 μg / mL bitter gourd extract + 1 × 10 6 CFU / mL Akkermansia]]>

[0150] The results are as follows Figure 1 (Positive group 1, type II diabetes model) Figure 2 (Positive group 2, type I diabetes model) As shown, the composition of the present invention can significantly promote glucose uptake by cells in zebrafish and has a potential hypoglycemic effect; that is, the composition of the present invention has a good hypoglycemic effect.

[0151] It will be understood by those skilled in the art that, unless otherwise defined, all terms used herein (including technical and scientific terms) have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. It should also be understood that terms such as those defined in general dictionaries should be understood to have the same meaning as in the context of the prior art, and should not be interpreted in an idealized or overly formal sense unless specifically defined as herein.

[0152] It should be understood that the above detailed description of the technical solutions of the present invention with reference to preferred embodiments is illustrative and not restrictive. Those skilled in the art can modify the technical solutions described in the embodiments or make equivalent substitutions for some of the technical features based on reading this specification; however, these modifications or substitutions do not cause the essence of the corresponding technical solutions to depart from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. An Akk bacteria composition containing plant extracts for regulating blood sugar, wherein, The Akk bacteria include live Akk bacteria and / or Akk postbiotics.

2. The composition according to claim 1, characterized in that, The Akk postbiotic is an inactivated bacterium, a lysed bacterium, or a culture medium containing dead Akk cells.

3. The composition according to claim 2, characterized in that, The inactivation methods of the Akk postbiotic are pasteurization, heating, ultraviolet irradiation, and chemical treatment, with pasteurization being the preferred method.

4. The composition according to claim 1, characterized in that, The plant extracts mentioned are bitter melon extract, mulberry leaf extract, coptis extract, gynostemma pentaphyllum extract, highland barley extract, potato extract, sugarcane extract, green tea extract, fenugreek extract, ivy extract, cactus extract, ginseng extract, cinnamon extract, garlic extract, grapefruit extract, monk fruit extract, raspberry extract, red ginseng extract, Solomon's seal extract, chia seed extract, yam extract, kudzu root extract, ophiopogon japonicus extract, garcinia cambogia extract, amla extract, grape seed extract, cranberry extract, white kidney bean extract, and red beet extract. Extracts, including black ginger extract, five-layered dragon extract, eucommia leaf extract, onion extract, wood ear fungus extract, okra extract, Jerusalem artichoke root extract, moth leaf extract, crape myrtle leaf extract, gymnospermum extract, blueberry extract, Chinese tallow tree root extract, centella asiatica leaf extract, panna coccinea extract, holy basil leaf extract, clove extract, bayberry extract, eucalyptus extract, tartary buckwheat extract, purslane extract, Agaricus blazei extract, dandelion extract, cherry extract, sea buckthorn oil extract, American ginseng extract, corn silk extract, and mulberry extract.

5. The composition according to claim 4, characterized in that, The active ingredients of the plant extract are one or more of the following: momordicin, momordicin, alkaloids, carotene, bitter principles, momordicin saponins, momordic polypeptides, glucosides, mulberry polysaccharides, gypsum acid, anthocyanins, ginkgo flavonoids, terpene lactones, corosolic acid, flavonoids, berberine, quercetin, ursolic acid, oleanolic acid, myricetin, organic acids, volatile oils, polysaccharides, terpenoids, flavonoids, phenolic compounds, alkaloids, glycosides, sterols, minerals, quinones, lignans, coumarins, saponins, cardiac glycosides, phenolic acids, amino acids, 1-deoxynojirimycin (DNJ), puerarin, and alkaloids.

6. The composition according to claim 4, characterized in that, The active ingredients of the bitter melon extract are one or more of the following: momordicin, momordicin, alkaloids, carotene, bitter principles, momordic saponins, momordic polypeptides, and glucosides.

7. The composition according to claim 4, characterized in that, The active ingredients of the mulberry leaf extract are one or more of 1-deoxynojirimycin (DNJ), mulberry leaf polysaccharides, mulberry leaf flavonoids, alkaloids, and mulberry leaf polyphenols.

8. The composition according to claim 4, characterized in that, The content of the plant extract is ≥1 mg / serving.

9. The composition according to claim 1, characterized in that, It also includes other probiotics that are beneficial to the gut, namely one or more of the genera *Lactobacillus*, *Bifidobacterium*, *Lactobacillus*, *Lactobacillus*, *Enterococcus*, *Bacillus*, *Lactobacillus mucosae*, *Streptococcus*, and *Lactococcus*.

10. The composition according to claim 9, characterized in that, The other probiotics are one or more of the following: *Lactobacillus paracasei*, *Bifidobacterium animalis* subsp. *lactobacter*, *Lactobacillus acidophilus*, *Lactobacillus casei*, *Lactobacillus plantarum*, *Enterococcus faecalis*, *Bifidobacterium longum* subsp. *longum*, *Bifidobacterium longum* subsp. *infant*, *Lactobacillus rhamnosus*, *Bifidobacterium adolescentis*, *Bifidobacterium animalis* subsp. *animal*, *Bifidobacterium bifidum*, *Bifidobacterium pseudosporidis*, *Bacillus subtilis*, *Bacillus subtilis*, *Bacillus subtilis*, *Bacillus subtilis*, *Bacillus subtilis*, *Bacillus subtilis*, *Bacillus subtilis*, *Bacillus subtilis*, *Lactobacillus subtilis*, *Lactobacillus curvatureii*, lactic acid bacteria, *Lactococcus lactis*, *Lactobacillus gasseri*, *Lactobacillus johnsonii*, *Lactobacillus maltii* subsp. *maltii*, *Pediococcus pentosaceus*, *Pediococcus lactis*, *Streptococcus faecalis*, *Bacillus coagulans*, *Bacillus licheniformis*, *Bacillus natto*, *Clostridium butyricum*, *Vibrio butyricum*, and lactic acid bacteria.

11. The composition according to claim 1, characterized in that, It also includes trace elements, and further, the trace elements are one or more of chromium, zinc, magnesium, and manganese.

12. The composition according to claim 1, characterized in that, It also includes fungi, and further, the fungi are one or more of Chaga mushroom, Grifola frondosa, Hericium erinaceus, and Cordyceps sinensis.

13. The composition according to any one of claims 1-12, characterized in that, The composition, when combined with excipients, is prepared into one or more of the following: tablets, soft capsules, capsules, powders, oral liquids, sprays, solid beverages, gummies, jellies, and crystals.

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