A partitioned rotating bioreactor for organic waste gas biological purification and resource utilization and an application method thereof

Abstract

The application discloses a partition rotating biological reactor for organic waste gas biological purification and resource utilization and an application method thereof. The reactor comprises an integrated rotating bed treatment unit, a dispersed rotating disc treatment unit, a spraying liquid supply system and a visible light introduction system. The integrated rotating bed treatment unit comprises an integrated rotating bed closed cavity, an integrated rotating bed rotating shaft and an integrated rotating bed filler bed fixed to the integrated rotating bed rotating shaft. The integrated rotating bed rotating shaft is a hollow rotating shaft, and a plurality of air inlets are arranged on the shaft section inside the cavity. The dispersed rotating disc treatment unit comprises a dispersed rotating disc closed cavity, a dispersed rotating disc rotating shaft and an algal-bacterial symbiotic body attached rotating disc. The algal-bacterial symbiotic body attached rotating disc is arranged at intervals along the axial direction of the dispersed rotating disc rotating shaft, and the surface of the algal-bacterial symbiotic body attached rotating disc is loaded with algal-bacterial symbiotic biofilm. The application has high mass transfer efficiency and is not prone to blockage, and has the functions of organic waste gas removal and CO2 fixation into resource products.

Description

Technical Field

[0001] This invention belongs to the field of organic waste gas biological purification and resource utilization technology, specifically relating to a partitioned rotating bioreactor for organic waste gas biological purification and resource utilization and its application method. Background Technology

[0002] Organic waste gases are widely generated in industries such as chemical engineering, printing, coating, pharmaceuticals, food processing, wastewater treatment, and organic solid waste treatment. Organic waste gases are characterized by complex compositions and the toxicity or odor of some substances. If emitted directly without effective treatment, they will not only cause air pollution but may also have adverse effects on human health and the ecological environment.

[0003] Traditional organic waste gas treatment technologies mainly include adsorption, condensation, combustion, photocatalytic oxidation, plasma treatment, and biological treatment. Traditional physicochemical technologies for treating organic waste gas typically suffer from high operating costs, high energy consumption, and the potential generation of secondary pollutants or waste. In contrast, biological treatment technologies have gained widespread attention due to their advantages such as low operating costs, less secondary pollution, and suitability for continuous treatment of low- to medium-concentration organic waste gases.

[0004] Existing common biological treatment devices mainly include biofilters, biotrickling filters, and bioscrubbers. However, these devices still suffer from problems such as packing blockage, limited gas-liquid mass transfer efficiency, and uneven nutrient solution distribution during long-term operation. In addition, the CO2 generated during the biodegradation of organic waste gas is usually not further utilized or fixed, which limits the application potential of such devices in the treatment of low-carbon waste gas.

[0005] Therefore, there is an urgent need to design a partitioned rotating bioreactor for the biological purification and resource recovery of organic waste gas in order to overcome the above-mentioned technical defects. Summary of the Invention

[0006] To address the technical problems of existing biological treatment devices during long-term operation, such as packing blockage, limited gas-liquid mass transfer efficiency, uneven nutrient solution distribution, and the fact that CO2 generated during the biodegradation of organic waste gas is often not further utilized or fixed, this invention provides a partitioned rotating bioreactor and its application method that integrates the functions of biological purification of organic waste gas, CO2 emission reduction, and carbon resource recovery. After treatment by the device described in this invention, VOCs and odor components in the effluent can be effectively removed, and the CO2 concentration can be significantly reduced to near or below the environmental background level, thereby meeting the requirements for air recycling. Simultaneously, the carbon resources in the waste gas can be efficiently converted and enriched in algal biomass, and the obtained algal lipids can be further refined and converted into high-value-added resource products such as biodiesel.

[0007] The technical solution adopted in this invention is: The first aspect of this invention relates to a partitioned rotary bioreactor for the biological purification and resource utilization of organic waste gas, characterized in that it comprises an integrated rotating bed treatment unit, a decentralized rotating disc treatment unit, a spray liquid supply system, and a visible light introduction system; the integrated rotating bed treatment unit and the decentralized rotating disc treatment unit are connected by a gas connection pipeline (24), and the organic waste gas to be treated sequentially enters the integrated rotating bed treatment unit and the decentralized rotating disc treatment unit for staged purification; wherein: The integrated rotating bed treatment unit includes an integrated rotating bed sealed cavity (5), an integrated rotating bed shaft (1), and an integrated rotating bed packing bed (4) fixed to the integrated rotating bed shaft (1); the integrated rotating bed shaft (1) is a hollow shaft, and multiple air inlets (3) are provided on the shaft section located inside the cavity, so that the organic waste gas to be treated can be evenly introduced into the area where the packing bed (27) is located through the air inlets (3); The decentralized rotary processing unit includes a decentralized rotary sealed cavity (21), a decentralized rotary shaft (12), and an algae-bacterial symbiotic loading rotary disk (14). The algae-bacterial symbiotic loading rotary disk (14) is arranged at intervals along the axial direction of the decentralized rotary shaft (12), and the surface of the algae-bacterial symbiotic loading rotary disk (14) is loaded with an algae-bacterial symbiotic biofilm.

[0008] Furthermore, the two ends of the integrated rotary bed shaft (1) are sealed and pivotally inserted into the sealed cavity (5) of the integrated rotary bed. One end is connected to the sealed cavity (5) of the integrated rotary bed through a leak-proof bearing (2), and the other end extends to the outside of the sealed cavity (5) of the integrated rotary bed and is connected to the rotary bed drive motor (11) for transmission, so as to drive the integrated rotary bed shaft (1) to rotate. The two ends of the distributed turntable shaft (12) are pivotally inserted into the sealed cavity (21) of the distributed turntable, and the two ends extend out of the sealed cavity (21). One end extends out of the sealed cavity (21) of the distributed turntable and is connected to the turntable frequency conversion motor (28) for driving the distributed turntable shaft (12) to rotate.

[0009] Furthermore, the integrated rotating bed packing bed (4) is fixed on the integrated rotating bed shaft (1) and rotates synchronously with the integrated rotating bed shaft (1); the integrated rotating bed packing bed (4) includes a rotating bed packing area (27), which is filled with a combination of hydrophilic sponge packing and hydrophobic polyurethane packing, with a mass ratio of 1:1.

[0010] Furthermore, the integrated rotating bed cavity (5) is provided with an integrated rotating bed air outlet (10) on the side near the decentralized rotating disk processing unit, and the decentralized rotating disk cavity (21) is provided with a decentralized rotating disk air inlet (13) on the side near the integrated rotating bed processing unit and a decentralized rotating disk air outlet (20) on the side away from the integrated rotating bed processing unit; the integrated rotating bed air outlet (10) and the decentralized rotating disk air inlet (13) are connected by a gas connection pipeline (24).

[0011] Furthermore, the distance between two adjacent algae-bacterial symbiotic loading turntables (14) is 1.5~2 cm; the surface of the algae-bacterial symbiotic loading turntables (14) is fixed with a porous fiber-based material with a thickness of 0.5~1 mm, which is used to load algae-bacterial symbiotic biofilm.

[0012] Furthermore, the porous fiber-based material is obtained by impregnating porous packing material with a composite bacterial culture solution; the composite bacterial culture solution is a mixture of bacterial cultures obtained from the fermentation and cultivation of individual composite bacterial cultures, and the composite bacterial cultures include: Acinetobacter veneriense (… Acinetobacter venetianus CCTCC NO: M2022719 (disclosed in patent application CN202211294292.5), *Aeromonas cuspidatum* ( Zoogloea resiniphila CCTCC NO: M2012235 (disclosed in patent application CN201310281412.2) 、 Acinetobacter moderosus ( Acinetobacter modestus CCTCCNO: M2021800 (disclosed in patent application CN202111038597.5), Trichomonas testis ( Comamonas testosteroni CCTCC NO: M20251624 (disclosed in patent application CN202511962261.6), *Pseudomonas montelukast* ( Pseudomonas monteilii CCTCC NO: M2023241 (disclosed in patent application CN202310323095.X).

[0013] The composite bacterial culture contains Acinetobacter vesicanthii CCTCC NO: M2022719, Trichomonas testis CCTCC NO: M20251624, and Agrobacterium tumefaciens CCTCC NO: M2012235. 、 After adjusting the fermentation cultures of Acinetobacter moderosus (CCTCC NO: M2021800) and Pseudomonas montmorilloni (CCTCC NO: M2023241) to the same OD value (preferred OD value), the broths were fermented separately. 600 (Value 0.3), and equal volumes of mixed bacterial solutions.

[0014] More preferably, the mixed porous packing material with attached composite microorganisms is prepared according to the following method: (1) Acinetobacter veneris CCTCC NO: M2022719 was inoculated into an inorganic salt medium containing 50 mg / L cyclohexane; Trichomonas testis CCTCC NO: M20251624 was inoculated into an inorganic salt medium containing 50 mg / L chlorobenzene; Agrobacterium tumefaciens CCTCC NO: M2012235 was inoculated into an inorganic salt medium containing 50 mg / L o-xylene; Acinetobacter modestros CCTCC NO: M2021800 was inoculated into an inorganic salt medium containing 50 mg / L butyl acetate; Pseudomonas montelukas CCTCC NO: M2023241 was inoculated into an inorganic salt medium containing 50 mg / L ethanol; each medium was sealed and placed in a shaker for incubation at 160 rpm and 30℃ for 48-72 h to obtain Acinetobacter veneris CCTCC NO: M2022719 bacterial suspension (OD). 600 = 0.3~0.4), Trichomonas vaginalis CCTCC NO: M20251624 (OD 600 = 0.3~0.4), Bacteroides citrate CCTCC NO:M2012235 bacterial suspension (OD) 600 = 0.3~0.4) 、 Acinetobacter moderators CCTCC NO: M2021800 bacterial culture (OD) 600 =0.6~0.7) and Pseudomonas montelukastii CCTCC NO: M2023241 bacterial suspension (OD 600 = 0.6~0.7). (2) The bacterial suspensions of Acinetobacter venetum (CCTCC NO: M2022719), Trichomonas testis (CCTCC NO: M20251624), and Agrobacterium tumefaciens (CCTCC NO: M2012235) were prepared. 、 The OD values ​​of Acinetobacter moderosite CCTCC NO: M2021800 and Pseudomonas montmorillonite CCTCC NO: M2023241 bacterial suspensions were diluted to the same value and mixed at a volume ratio of 1:1:1:1:1 to obtain a mixed bacterial suspension. (3) The mixed porous packing material (a 1:1 mixture of hydrophilic sponge and hydrophobic polyurethane) is immersed in the mixed bacterial solution prepared in step (2) and immersed at room temperature for 24 h to obtain the packing material with the mixed bacterial body attached; the volume ratio of the packing material to the mixed bacterial solution is 1:2-4.

[0015] Preferably, the porous fiber-based material on the attached algae-bacteria rotating disc is obtained by impregnation with a mixed algae-bacteria solution; the bacterial solution is the mixed bacterial solution prepared in step (2); the mixed algae solution is a mixture of algae solutions obtained from the cultivation of each of the mixed algae, and the mixed algae include Chlorella proteoglycans (…). Chlorella sp. FACHB-10, Sorokin Chlorella ( Chlorella sorokiniana ) FACHB-26, Chlorella vulgaris ( Chlorella vulgaris FACHB-36 or Chlorella vulgaris ( Chlorella ellipsoidea FACHB-42 were all purchased from the Freshwater Algae Culture Collection of the Chinese Academy of Sciences.

[0016] Preferably, the mixed algal solution is composed of Chlorella proteoglycans (…). Chlorella sp. FACHB-10, Sorokin Chlorella ( Chlorella sorokiniana ) FACHB-26, Chlorella vulgaris ( Chlorella vulgaris FACHB-36 or Chlorella vulgaris ( Chlorella ellipsoidea After adjusting each algal solution of FACHB-42 to the same OD value (preferred OD value) 680 All are 0.8), and are mixed in equal volumes.

[0017] Preferably, the algal solution is mixed with the bacterial solution obtained in step (2) to prepare a mixed algal-bacterial solution; the mixed algal-bacterial solution is added to a dispersive rotating disk, and the porous fiber-based material is immersed in the mixed algal-bacterial solution. The mixture is rotated and immersed for 24 h under room temperature and closed conditions, so that the algal-bacterial symbiont attaches to the surface of the porous fiber-based material, thereby obtaining a porous fiber-based material with attached algal-bacterial symbiont; wherein, the volume ratio of the mixed algal-bacterial solution to the porous fiber-based material is 1:0.1~0.5.

[0018] Preferably, the mixed algal solution is prepared according to the following method: 1) Chlorella pyrenoidosa FACHB-10, Chlorella sorokinosa FACHB-26, Chlorella vulgaris FACHB-36, or Chlorella ellipsoidosa FACHB-42 species respectively (preferably with an initial cell mass of 10). 7 Cells / mL) were inoculated into Erlenmeyer flasks containing algal culture medium and cultured at 30°C for 24-48 h under a 16 h light / 8 h dark incubator to obtain *Chlorella proteoglycans* FACHB-10, *Chlorella sorokinica* FACHB-26, *Chlorella vulgaris* FACHB-36, or *Chlorella ellipsoides* FACHB-42 algal solutions, respectively. 680 All are 0.8-1.0; 2) Adjust the OD of Chlorella proteoglycans FACHB-10, Chlorella sorokinica FACHB-26, Chlorella vulgaris FACHB-36, or Chlorella ellipsoides FACHB-42 algal solutions.680 After the values ​​are the same, they are mixed at a volume ratio of 1:1:1:1 to obtain a mixed algal solution.

[0019] Preferably, the algal culture medium comprises: NaNO3 1.5 g / L, K2HPO4·3H2O 0.04 g / L, MgSO4·7H2O 0.075 g / L, CaCl2·2H2O 0.036 g / L, CaCl2 0.006 g / L, C6H8FeNO7 0.006 g / L, EDTA 0.001 g / L, Na2CO3 0.02 g / L, H3BO3 0.00286 g / L, MnCl2·H2O 0.00181 g / L, ZnSO4·7H2O 0.000222 g / L, CuSO4·5H2O 0.000079 g / L, Na2MoO 4· The mixture was sterilized by autoclaving at 121°C for 20 min with 0.000039 g / L of 2H2O and 0.000049 g / L of CoNO3·6H2O in deionized water.

[0020] Furthermore, it also includes a spray liquid supply system and a visible light guiding system; among which: The spray supply system includes an integrated rotating bed spray system (9) and a dispersed rotating disc spray system (16). The integrated rotating bed spray system (9) and the dispersed rotating disc spray system (16) are located at the top of the integrated rotating bed cavity (5) and the dispersed rotating disc cavity (21), respectively, and are used to spray inorganic salt culture medium, nutrient solution or circulating liquid onto the integrated rotating bed packing bed (4) and the algae-bacterial symbiotic loading disc (14). The visible light import system is set in the sealed cavity (21) of the distributed turntable and includes a plant growth supplement light lamp. The plant growth supplement light lamp is set towards the algae-bacterial symbiotic loading turntable (14) and is used to provide the light required for photosynthesis of green algae in the algae-bacterial symbiotic biofilm.

[0021] Preferably, the inorganic salt culture medium has the following composition: K₂HPO₄ 1.942 g / L, KH₂PO₄ 0.234 g / L, NaNO₃ 1.7 g / L, NH₄Cl 0.98 g / L, MgCl₂ 6H₂O 0.2033 g / L, CaCl₂ The micronutrient solution contained 0.0111 g / L ZnCl2 and 0.0162 g / L FeCl3, with a concentration of 5 mL / L, and deionized water as the solvent. 4H2O 0.060 g / L, KI 0.01 g / L, NaMO4 Sterilize with 0.1 g / L 2H2O and 0.05 g / L H3BO3 in deionized water at 110℃ for 40 min by autoclaving.

[0022] Preferably, the spraying intensity of the inorganic salt culture medium is 0.25-0.75 m. 3 / (m 2 h), use intermittent spraying, spraying for 1-5 minutes every half hour.

[0023] Preferably, both the integrated rotating bed and the dispersed rotating disc can adjust the nutrient solution level through the corresponding outlet on the side wall of the cavity to achieve two operating modes: submersion supply and spray supply, and to regulate the contact volume between the packing bed or rotating disc assembly and the accumulated nutrient solution. The integrated rotating bed operates at a speed of 1-10 r / min, used to enhance packing bed wetting, VOCs mass transfer, and stable attachment of functional bacteria under low-speed rotation conditions. The dispersed rotating disc has two speed modes: low speed (1-10 r / min) to promote the attachment, growth, and stable operation of algal biofilms, and high speed (100-500 r / min) to shear the mature algal biofilm, enabling controlled desorption and harvesting of algal biomass.

[0024] Furthermore, the bottom of the integrated rotating bed sealed cavity (5) is provided with an integrated rotating bed bottom outlet (6), and the side wall is provided with an integrated rotating bed side outlet (7) for liquid discharge, liquid reflux and liquid level control; the bottom of the decentralized rotating disc sealed cavity (21) is provided with a decentralized rotating disc bottom outlet (17), and the side wall is provided with a decentralized rotating disc side outlet (18) for liquid discharge, liquid reflux and liquid level control.

[0025] An integrated rotating bed water storage area (22) is provided above the integrated rotating bed spray system (9), and an integrated rotating bed water inlet pipe (8) is provided at the top of the integrated rotating bed water storage area (22); a decentralized rotating disc water storage area (23) is provided above the decentralized rotating disc spray system (16), and a decentralized rotating disc water inlet pipe (15) is provided at the top of the decentralized rotating disc water storage area (23).

[0026] A second aspect of the present invention relates to a method for treating organic waste gas using the aforementioned partitioned rotating bioreactor, characterized by comprising the following specific steps: S1. The organic waste gas to be treated is introduced into the sealed cavity of the integrated rotating bed through the air inlet, so that it comes into contact with the composite bacteria loaded on the surface of the integrated rotating bed packing bed (4). At the same time, the integrated rotating bed spraying system (9) is turned on to spray the inorganic salt culture medium, nutrient solution or circulating liquid evenly onto the packing area (27) to achieve primary absorption, mass transfer and biodegradation of organic waste gas. S2. The residual organic waste gas after primary purification enters the sealed cavity of the integrated rotating bed through the gas connection pipeline (24) and comes into contact with the algae-bacterial symbiotic biofilm on the surface of the algae-bacterial symbiotic loading disc (14). At the same time, the decentralized rotating disc spraying system (16) is turned on to spray inorganic salt culture medium, nutrient solution or circulating liquid onto the surface of each algae-bacterial symbiotic loading disc (14) in a directional manner to maintain the activity and stability of the algae-bacterial symbiotic biofilm. At the same time, under light conditions and low-speed purification film attachment mode, the functional bacteria in the algae-bacterial symbiotic biofilm further degrade the residual waste gas, while the microalgae use the CO2 generated during the waste gas mineralization process to assimilate and fix it through photosynthesis. S3. After the organic waste gas treatment is running stably, switch the decentralized rotary disc treatment unit to the high-speed biomass harvesting mode, with a rotation speed of 100~500 r / min, so that the algae-bacterial symbiotic biofilm detaches from the surface of the algae-bacterial symbiotic rotating disc under shearing action; collect the detached algae-bacterial biomass, and obtain algal lipids after centrifugation, drying, grinding and organic solvent extraction.

[0027] Furthermore, the rotational speed of the integrated rotating bed's sealed cavity is 1~10 r / min, and the spray intensity of the spray system is 0.25~0.75 m. 3 / (m²·h), the liquid immersion rate of the rotating bed is 0~100%; the decentralized rotating disc has a low-speed purification film-forming mode and a high-speed biomass harvesting mode, wherein the rotation speed in the low-speed purification film-forming mode is 1~10 r / min, the spray intensity of the spray system is 0.25~0.75 m³ / (m²·h), and the liquid immersion rate is 0~100%.

[0028] Furthermore, the organic waste gas includes one or more of cyclohexane, chlorobenzene, o-xylene, butyl acetate, and ethanol; in the treatment, the composite microbial community degrades the organic waste gas and generates CO2, and the green algae in the algae-bacterial symbiotic biofilm are used to assimilate and fix CO2 and convert some carbon resources into algal lipids.

[0029] Compared with the prior art, the beneficial effects of the present invention are reflected in: (1) The partitioned rotating bioreactor provided by the present invention achieves graded absorption, mass transfer and biodegradation of VOCs waste gas by connecting an integrated rotating bed treatment unit and a decentralized rotating disk treatment unit in series, thereby improving the purification efficiency of organic waste gas and the stability of system operation.

[0030] (2) The present invention enables the biofilm to periodically contact gaseous pollutants and liquid nutrient solution through the synergistic effect of rotating packing bed, dispersed rotating disk, spray liquid supply and adjustable liquid level structure, thereby enhancing the gas-liquid-biofilm mass transfer efficiency and reducing the risk of packing blockage.

[0031] (3) The present invention constructs an algae-bacteria symbiotic system in the right cavity distributed rotary disk treatment unit, which can further degrade residual VOCs and assimilate and fix CO2 generated by VOCs mineralization, converting some carbon resources into algal biomass and algal lipids, thereby achieving synergistic treatment of organic waste gas purification, CO2 emission reduction and carbon resource recovery.

[0032] (4) The partitioned rotating bioreactor described in this invention can be used for the purification of low-concentration, high-volume organic waste gas generated in industries such as chemical, pharmaceutical, spraying, rubber, food processing and sewage treatment. While achieving efficient removal of VOCs, it can also biologically fix CO2 generated during the process of waste gas purification and pollutant mineralization, and further convert waste gas carbon resources into algal biomass and algal lipids, thereby achieving the synergistic unity of organic waste gas pollution control, carbon emission reduction and biomass energy recovery.

[0033] (5) The reactor of the present invention consists of an integrated rotating bed and a decentralized rotating disc. The integrated rotating bed achieves primary biological purification of organic waste gas by loading microorganisms with specific pollutant degradation capabilities. The decentralized rotating disc achieves secondary deep purification of residual organic waste gas, CO2 biological fixation, and directional conversion of waste gas carbon resources into algal lipids by constructing an algae-bacteria symbiotic system composed of pollutant-degrading microorganisms and green algae. Attached Figure Description

[0034] Figure 1 Schematic diagram of a zoned rotating bioreactor device; Explanation of reference numerals in the attached figures: 1. Integrated rotating bed shaft; 2. Leak-proof bearing; 3. Air inlet; 4. Integrated rotating bed packing bed; 5. Integrated rotating bed sealed cavity; 6. Integrated rotating bed bottom outlet; 7. Integrated rotating bed side outlet; 8. Integrated rotating bed water inlet pipe; 9. Integrated rotating bed spray system; 10. Integrated rotating bed air outlet; 11. Rotary bed drive motor; 12. Distributed rotating disc shaft; 13. Distributed rotating disc air inlet; 14. Algae-bacterial symbiotic loading rotating disc; 15. 16. Distributed rotating disc water inlet pipe; 17. Distributed rotating disc spray system; 18. Distributed rotating disc bottom outlet; 19. Distributed rotating disc side outlet; 20. Distributed rotating disc sampling port; 21. Distributed rotating disc air outlet; 22. Distributed rotating disc sealed cavity; 23. Integrated rotating bed water storage area; 24. Distributed rotating disc water storage area; 25. Gas connection pipeline; 26. Distributed rotating disc upper shell; 27. Distributed rotating disc lower shell; 28. Rotating bed packing area; 29. ​​Rotary disc variable frequency motor.

[0035] Figure 2 This is a schematic diagram of the packing column of an integrated rotating bed in the left chamber.

[0036] Figure 3 This is a schematic diagram of a rotating disk for attaching right ventricular algae symbionts.

[0037] Figure 4 The removal efficiency of chlorobenzene in a partitioned rotating bioreactor.

[0038] Figure 5 The carbon dioxide fixation rate of the partitioned rotating bioreactor.

[0039] Figure 6 The algal lipid recovery rate of a zoned rotating bioreactor. Detailed Implementation

[0040] The specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are for illustration and explanation only and are not intended to limit the scope of the present invention.

[0041] It should be noted that, unless otherwise specified, the embodiments and features described in the present invention can be combined with each other.

[0042] The present invention will now be described in detail with reference to the accompanying drawings and exemplary embodiments.

[0043] Example 1: Design of a Rotary Partitioned Bioreactor

[0044] refer to Figures 1 to 3 This invention discloses a partitioned rotary bioreactor for the biological purification and resource utilization of organic waste gas, comprising an integrated rotating bed treatment unit, a decentralized rotating disc treatment unit, a spray liquid supply system, and a visible light introduction system; the integrated rotating bed treatment unit and the decentralized rotating disc treatment unit are connected by a gas connection pipeline 24, and the organic waste gas to be treated sequentially enters the integrated rotating bed treatment unit and the decentralized rotating disc treatment unit for staged purification; wherein: The integrated rotating bed treatment unit includes an integrated rotating bed sealed cavity 5, an integrated rotating bed shaft 1, and an integrated rotating bed packing bed 4 fixed to the integrated rotating bed shaft 1; the integrated rotating bed shaft 1 is a hollow shaft, and multiple air inlets 3 are provided on the shaft section located inside the cavity, and the organic waste gas to be treated enters the area where the packing bed 27 is located evenly through the air inlets 3. The decentralized rotary processing unit includes a decentralized rotary sealed cavity 21, a decentralized rotary shaft 12, and an algae-bacterial symbiotic loading rotary disk 14. The algae-bacterial symbiotic loading rotary disk 14 is arranged at intervals along the axial direction of the decentralized rotary shaft 12, and the surface of the algae-bacterial symbiotic loading rotary disk 14 is loaded with an algae-bacterial symbiotic biofilm.

[0045] In this embodiment, the two ends of the integrated rotary bed shaft 1 are pivotally and sealed into the sealed cavity 5 of the integrated rotary bed. One end is connected to the sealed cavity 5 of the integrated rotary bed through an air-proof bearing 2, and the other end extends to the outside of the sealed cavity 5 of the integrated rotary bed and is connected to the rotary bed drive motor 11 for transmission, so as to drive the integrated rotary bed shaft 1 to rotate. The two ends of the distributed turntable shaft 12 are pivotally inserted into the sealed cavity 21 of the distributed turntable, and both ends extend out of the sealed cavity 21 of the distributed turntable. One end extends out of the sealed cavity 21 of the distributed turntable and is connected to the turntable frequency conversion motor 28 for transmission, so as to drive the distributed turntable shaft 12 to rotate.

[0046] In this embodiment, the integrated rotating bed packing 4 is fixed on the integrated rotating bed shaft 1 and rotates synchronously with the integrated rotating bed shaft 1. The integrated rotating bed packing 4 includes a rotating bed packing area 27, which is filled with a combination of hydrophilic sponge packing and hydrophobic polyurethane packing in a mass ratio of 1:1. The packing volume is preferably two-thirds of the internal volume of the packing column.

[0047] In this embodiment, the integrated rotating bed cavity 5 is provided with an integrated rotating bed air outlet 10 on the side near the decentralized rotating disk processing unit, the decentralized rotating disk cavity 21 is provided with a decentralized rotating disk air inlet 13 on the side near the integrated rotating bed processing unit, and a decentralized rotating disk air outlet 20 on the side away from the integrated rotating bed processing unit; the integrated rotating bed air outlet 10 and the decentralized rotating disk air inlet 13 are connected by a gas connection pipe 24.

[0048] In this embodiment, the distance between two adjacent algae-bacterial symbiotic loading turntables 14 is 1.5~2 cm; the surface of the algae-bacterial symbiotic loading turntable 14 is fixed with a porous fiber-based material with a thickness of 0.5~1 mm, which is used to load algae-bacterial symbiotic biofilm.

[0049] In this embodiment, a spray liquid supply system and a visible light guiding system are also included; wherein: The spray supply system includes an integrated rotating bed spray system 9 and a dispersed rotating disc spray system 16. The integrated rotating bed spray system 9 and the dispersed rotating disc spray system 16 are located at the top of the integrated rotating bed cavity 5 and the dispersed rotating disc cavity 21, respectively, and are used to spray inorganic salt culture medium, nutrient solution or circulating liquid onto the integrated rotating bed packing bed 4 and the algae-bacterial symbiotic loading disc 14. The visible light import system is set inside the distributed turntable sealed cavity 21 and includes a plant growth supplement light. The plant growth supplement light is set towards the algae-bacterial symbiotic loading turntable 14 and is used to provide the light required for photosynthesis of green algae in the algae-bacterial symbiotic biofilm.

[0050] Preferably, the spraying intensity of the inorganic salt culture medium is 0.25-0.75 m. 3 / (m 2 h), use intermittent spraying, spraying for 1-5 minutes every half hour.

[0051] Preferably, both the integrated rotating bed and the dispersed rotating disc can adjust the nutrient solution level through the corresponding outlet on the side wall of the cavity to achieve two operating modes: submersion supply and spray supply, and to regulate the contact volume between the packing bed or rotating disc assembly and the accumulated nutrient solution. The integrated rotating bed operates at a speed of 1-10 r / min, used to enhance packing bed wetting, VOCs mass transfer, and stable attachment of functional bacteria under low-speed rotation conditions. The dispersed rotating disc has two speed modes: low speed (1-10 r / min) to promote the attachment, growth, and stable operation of algal biofilms, and high speed (100-500 r / min) to shear the mature algal biofilm, enabling controlled desorption and harvesting of algal biomass.

[0052] In this embodiment, the bottom of the integrated rotating bed sealed cavity 5 is provided with an integrated rotating bed bottom outlet 6, and the side wall is provided with an integrated rotating bed side outlet 7, which are used for liquid discharge, liquid reflux and liquid level control; the bottom of the decentralized rotating disc sealed cavity 21 is provided with a decentralized rotating disc bottom outlet 17, and the side wall is provided with a decentralized rotating disc side outlet 18, which are used for liquid discharge, liquid reflux and liquid level control.

[0053] An integrated rotating bed water storage area 22 is provided above the integrated rotating bed spray system 9, and an integrated rotating bed water inlet pipe 8 is provided on the top of the integrated rotating bed water storage area 22; a decentralized rotating disc water storage area 23 is provided above the decentralized rotating disc spray system 16, and a decentralized rotating disc water inlet pipe 15 is provided on the top of the decentralized rotating disc water storage area 23.

[0054] Specifically, the total effective volume of the partitioned rotating bioreactor of this invention is 66 L, of which the effective volume of the integrated rotating bed treatment unit is 55 L and the effective volume of the decentralized rotating disc treatment unit is 11 L; the packing column volume is 12.26 L, the diameter of the algae-bacterial symbiotic loading rotating disc is 0.16 m, and the total loading area is 2.6 m². 2 .

[0055] Example 2: Construction of a complex microbial community

[0056] ①Propagation of degrading bacteria

[0057] Prepare an inorganic salt culture medium, autoclave at 110℃ for 40 min, and then dispense into shake flasks (50 mL each). Inoculate with Acinetobacter venetum (CCTCC NO: M2022719), Trichomonas vaginalis (CCTCC NO: M20251624), and Aerogenes foetida (CCTCC NO: M2012235), respectively. 、 Acinetobacter moderosus CCTCC NO: M2021800 and Pseudomonas montelukastii CCTCC NO: M2023241 were cultured in a shaker at 160 rpm and 30℃ for 48-72 h using cyclohexane, chlorobenzene, o-xylene, butyl acetate, and ethanol as carbon sources, respectively, with a carbon source addition of 50 mg / L. The resulting culture yielded Acinetobacter venereum CCTCC NO: M2022719. 600 = 0.35), Trichomonas vaginalis CCTCC NO: M20251624 bacterial suspension (OD) 600 = 0.60), Bacteroides citrate CCTCC NO: M2012235 bacterial suspension (OD 600 = 0.35) 、 Acinetobacter moderators CCTCC NO: M2021800 bacterial culture (OD) 600 = 0.60) and Pseudomonas montmorillonite CCTCC NO: M2023241 bacterial suspension (OD 600 =0.60).

[0058] The bacterial suspensions of Acinetobacter vesicanthii (CCTCC NO: M2022719), Trichomonas testis (CCTCC NO: M20251624), and Agrobacterium tumefaciens (CCTCC NO: M2012235) were tested. 、 Acinetobacter moderosus CCTCC NO: M2021800 bacterial suspension and Pseudomonas montelukast CCTCC NO: M2023241 bacterial suspension OD 600 The values ​​were all diluted to 0.35 and mixed at a volume ratio of 1:1:1:1:1 to obtain a mixed bacterial solution.

[0059] ② Filling and film formation of integrated rotating bed packing bed

[0060] Take 300 composite packing blocks with a size of 2 cm × 2 cm × 2 cm, including 150 hydrophilic sponge packing blocks and 150 hydrophobic polyurethane packing blocks, and immerse them all in 800 mL of mixed bacterial solution prepared in step 1. Let them stand at room temperature for 24 h to allow the mixed bacteria to fully adhere to the surface of the composite packing, thus obtaining a composite packing loaded with mixed bacteria. Subsequently, the composite packing loaded with mixed bacteria is filled into the left chamber integrated rotating bed packing (4) described in Example 1 to construct a microbial membrane packing bed with VOCs adsorption, mass transfer and biodegradation functions.

[0061] ③ Biofilm formation of mixed microbial communities in an integrated rotating bed

[0062] The composite packing material loaded with mixed bacteria is filled into the integrated rotating bed packing bed 4 in the left chamber, and fresh inorganic salt culture medium is added to the inorganic salt culture medium storage tank as the circulating nutrient solution for the integrated rotating bed spray system 9. The nutrient solution level in the chamber is adjusted so that one-third to one-half of the packing bed (4) is in contact with the nutrient solution accumulated at the bottom during rotation, so as to form a biofilm formation condition that combines spray supply and partial immersion supply.

[0063] During the biofilm formation process, the integrated rotating bed shaft 1 is turned on, and the rotation speed is controlled at 1~5 r / min to allow the composite packing material to rotate slowly. Simultaneously, the integrated rotating bed spray system 9 is turned on to intermittently spray inorganic salt culture medium onto the surface of the packing bed 4, preferably spraying for 3~5 minutes every 10 minutes, to maintain the surface of the packing material moist and provide nutrients and moisture for the attached microorganisms. The liquid level is adjusted through the side outlet 7 of the integrated rotating bed, and excess liquid is discharged or the nutrient solution is circulated back through the bottom outlet 6.

[0064] During the biofilm acclimation stage, simulated VOCs waste gas containing multiple components such as cyclohexane, chlorobenzene, o-xylene, butyl acetate, and ethanol is used as the acclimation gas source, with a preferred concentration of 50-200 mg / m³. This gas is introduced into the packing zone 27 through the air inlet 3 on the hollow rotating shaft of the integrated rotating bed, allowing the mixed microbial community to gradually adapt and form a stable biofilm under VOCs stress and inorganic nutrient supply. After 5-7 days of continuous operation, if the surface of the composite packing deepens in color, forms a uniform and moist adhesive layer, and the VOCs removal effect tends to stabilize, it indicates that the mixed microbial community has completed stable biofilm formation on the surface of the composite packing.

[0065] Example 3: Construction of Algal-Bacterial Symbiont

[0066] ①Algae propagation

[0067] Prepare algal culture medium and autoclave at 121℃ for 20 min. After cooling, dispense into Erlenmeyer flasks, each containing 500 mL. The initial cell concentration was 1.0 × 10⁻⁶ cells / mL. 7 cells / mL Chlorella sp.FACHB-10, Chlorella sorokiniana FACHB-26 Chlorella vulgaris FACHB-36 and Chlorella ellipsoidea FACHB-42 was inoculated into the aforementioned Erlenmeyer flasks and incubated in a 30°C constant temperature and light incubator. The incubation conditions were 12 h light / 12 h dark. After 10 days of incubation to the logarithmic growth phase, the following products were obtained: Chlorella sp. FACHB-10 algal solution, Chlorella sorokiniana FACHB-26 algae solution, Chlorella vulgaris FACHB-36 algae solution and Chlorella ellipsoidea FACHB-42 algal solution, and the OD of each algal solution 680 Adjust to 1.0. Set OD... 680 All are 1.0 Chlorella sp FACHB-10 algae solution, Chlorella sorokiniana FACHB-26 algae solution, Chlorella vulgaris FACHB-36 algae solution, Chlorella ellipsoidea FACHB-42 algal solutions were mixed in a volume ratio of 1:1:1:1 to obtain a mixed algal solution.

[0068] ② Preparation of algae-bacterial solution

[0069] The mixed bacterial solution obtained in step 1 of Example 2 is thoroughly mixed with the mixed algal solution obtained in step 1 above, and then mixed at a volume ratio of 1:0.5~2, preferably at a volume ratio of 1:1, to prepare the algae-bacteria mixed solution. During the mixing process, the OD of each functional strain in the mixed bacterial solution is measured. 600 All are 0.35, and the OD values ​​of each green algae solution in the mixed algae solution are... 680 All values ​​were 1.0. The resulting algae-bacterial mixture was allowed to stand at room temperature for 0.5–2 h to allow the functional bacteria to come into full contact with the green algae and form an initial algae-bacterial symbiotic system, thus obtaining the algae-bacterial solution for pre-coating the dispersed rotating algae-bacterial symbiotic system.

[0070] ③ Filling of the distributed rotating disc porous matrix material

[0071] The porous fiber-based material was cut into circular discs matching the size of the algae-bacterial symbiotic loading disc 14, and fixed onto the surface of 15 perforated stainless steel discs. Then, the algae-bacterial solution prepared in step 3 was added to the cavity of the dispersive disc. The liquid level of the algae-bacterial solution was adjusted through the side outlet, ensuring that the algae-bacterial symbiotic loading disc 14 was in a semi-submerged state during rotation. The dispersive disc shaft was turned on, and the rotation speed was controlled at 1-5 r / min. The disc was rotated and immersed at room temperature for 24 h, allowing the functional bacteria and green algae in the algae-bacterial solution to adhere to the surface of the porous fiber-based material, thus obtaining the algae-bacterial symbiotic loading disc with attached algae-bacterial symbionts.

[0072] ④ Biofilm formation of complex microbial communities and algae

[0073] During biofilm formation, the rotating shaft of the dispersed rotating disc is turned on, and the rotation speed is controlled at 1-5 r / min. Algal culture medium or inorganic salt culture medium is directionally sprayed onto the surface of each algal-bacterial symbiotic rotating disc 14 via the dispersed rotating disc spray system 16. Preferably, spraying is performed for 3-5 minutes every 30 minutes to maintain the moisture and growth activity of the algal-bacterial symbiotic biofilm. During the biofilm formation acclimatization stage, VOC-containing waste gas is first pre-treated by the integrated rotating bed in the left chamber, and then enters the dispersed rotating disc 21 in the right chamber through the gas connection pipe 24. The residual gas contains low concentrations of VOCs and CO2 produced during biodegradation. The residual VOCs can serve as acclimatization substrates for functional bacterial communities, and the CO2 can serve as an inorganic carbon source for green algae photosynthesis, thereby promoting the stable formation of the algal-bacterial symbiotic biofilm. During biofilm formation, light is provided to the right chamber via a visible light import system. The preferred light intensity for the algae-bacterial symbiotic loading disc 14 is 8000~20000 lx, and the preferred light-dark cycle is 12 h light / 12 h darkness or 18 h light / 6 h darkness. After 5~7 days of continuous operation, if a uniform green or yellowish-green biofilm forms on the surface of the algae-bacterial symbiotic loading disc 14, and the further removal of residual VOCs and CO2 assimilation in the right chamber tend to stabilize, it indicates that the algae-bacterial symbiotic has stably formed a biofilm on the porous fiber-based material surface.

[0074] Example 4: Evaluation of the treatment effect of a zoned rotating bioreactor on organic waste gas

[0075] Using the partitioned rotating bioreactors from Examples 2 and 3 that successfully attached biofilms, and simulating chlorobenzene as the inlet gas, the same operating mode as Examples 2 and 3 was employed to examine the purification effect of the device.

[0076] The concentrations of chlorobenzene in the inlet and outlet gases were detected using an Agilent gas chromatograph (Agilent 6890). The analytical conditions were as follows: (30 m × 0.25 mm × 0.5 μm) HP-Innowax column, injection port temperature 250 °C, detector (FID) temperature 250 °C, column temperature 200 °C, hydrogen flow rate 40 mL / min, air flow rate 450 mL / min, carrier gas nitrogen, column flow rate 0.9 mL / min, split ratio 20:1, and injection volume 800 μL. The concentration of carbon dioxide in the outlet gas was detected using an Agilent gas chromatograph (Agilent 6890). The analytical conditions were as follows: (30 m × 0.50 μm × 0.32 μm) HP-Innowax column, injection port temperature 100℃, detector (TCD) temperature 180℃, column temperature 42℃, helium make-up gas 7.0 mL / min, helium as carrier gas, column flow rate 0.2 mL / min, total flow rate 107 mL / min, split ratio 3.3:1, and injection volume 800 μL.

[0077] Figure 4 The purification effect of this device on chlorobenzene-containing waste gas shows that the removal rate of chlorobenzene remained above 90% for 40 days of operation. Based on the measured carbon dioxide content in the inlet and outlet gases, the theoretical and actual carbon dioxide yields of the reactor were calculated, and the results are shown in Table 1. It can be seen that the algal biofilm formed within the device can perform photosynthesis under simulated sunlight, fixing the carbon dioxide produced by the metabolism of the complex bacterial community and the carbon dioxide in the inlet gas. The amount of carbon dioxide detected in the outlet gas is significantly lower than the theoretical value.

[0078] Figure 5 Further analysis shows that the partitioned rotating bioreactor exhibits significant CO2 fixation capacity in all operating stages. Furthermore, the daily average CO2 fixation generally increases with increasing chlorobenzene inlet concentration, reaching its highest level in the fourth stage. In summary, the partitioned rotating bioreactor not only achieves efficient and stable VOCs removal over a wide range of chlorobenzene inlet concentrations but also significantly reduces the gaseous release of CO2 during chlorobenzene mineralization.

[0079] Table 1 Actual CO2 production and theoretical CO2 production at different stages

[0080] Example 5: Evaluation of the Resource Utilization Effect of a Rotary Partition Bioreactor

[0081] To evaluate the resource conversion capacity of the partitioned rotating bioreactor for waste gas carbon resources, algal and bacterial biomass in the right chamber dispersed rotating disc was harvested after each operating stage, and its algal lipid production rate was determined. The specific method is as follows: the right chamber dispersed rotating disc was switched from low-speed operation mode to high-speed biomass harvesting mode, and the rotation speed of the dispersed rotating disc was adjusted to 450 r / min, so that the algal symbiotic biofilm detached from the surface of the algal symbiotic loading disc (14) under high-speed shearing. The detached algal suspension was collected, and the algal biomass was recovered by centrifugation. The obtained biomass was then vacuum dried and ground into a uniform powder. A certain amount of dried algal biomass powder was taken and added to a chloroform / methanol mixed solvent (2:1, v / v) for algal lipid extraction. After solid-liquid separation, the organic phase was collected. The obtained algal lipid extract was subjected to colorimetric determination by the phosphate-vanillin method, and the algal lipid content was calculated according to the oil standard curve. The algal lipid production rate at different operating stages was further calculated. The changes in algal lipid production rate in the partitioned rotating bioreactor at different operating stages are as follows: Figure 6 As shown, with the advancement of the operation phase and the increase in chlorobenzene inlet load, the algal lipid production rate gradually increased, from approximately 8.2 mg / (L·d) in the first stage to approximately 20.5 mg / (L·d) in the fourth stage. The algal lipid production rates in the third and fourth stages were significantly higher than those in the first two stages, indicating that higher inlet air concentrations can provide more available carbon and CO2 sources for the algal-bacterial symbiotic system, thereby promoting algal-bacterial biomass accumulation and algal lipid synthesis.

[0082] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention.

Claims

1. A zoned rotating bioreactor for biological purification and resource recovery of organic waste gas, characterized in that, It includes an integrated rotating bed treatment unit, a decentralized rotating disc treatment unit, a spray liquid supply system, and a visible light introduction system; the integrated rotating bed treatment unit and the decentralized rotating disc treatment unit are connected by a gas connection pipeline (24), and the organic waste gas to be treated enters the integrated rotating bed treatment unit and the decentralized rotating disc treatment unit in sequence for graded purification; the spray liquid supply system is located at the top of the integrated rotating bed treatment unit and the decentralized rotating disc treatment unit respectively, and the visible light introduction system is set inside the decentralized rotating disc treatment unit, wherein: The integrated rotating bed treatment unit includes an integrated rotating bed sealed cavity (5), an integrated rotating bed shaft (1), and an integrated rotating bed packing bed (4) fixed to the integrated rotating bed shaft (1); the integrated rotating bed shaft (1) is a hollow shaft, and multiple air inlets (3) are provided on the shaft section located inside the cavity, so that the organic waste gas to be treated can be evenly introduced into the area where the packing bed (27) is located through the air inlets (3); The decentralized rotary processing unit includes a decentralized rotary sealed cavity (21), a decentralized rotary shaft (12), and an algae-bacterial symbiotic loading rotary disk (14). The algae-bacterial symbiotic loading rotary disk (14) is arranged at intervals along the axial direction of the decentralized rotary shaft (12), and the surface of the algae-bacterial symbiotic loading rotary disk (14) is loaded with an algae-bacterial symbiotic biofilm.

2. The partitioned rotating bioreactor according to claim 1, characterized in that, The two ends of the integrated rotary bed shaft (1) are sealed and pivotally inserted into the sealed cavity (5) of the integrated rotary bed. One end is connected to the sealed cavity (5) of the integrated rotary bed through a leak-proof bearing (2), and the other end extends to the outside of the sealed cavity (5) of the integrated rotary bed and is connected to the rotary bed drive motor (11) for transmission, so as to drive the integrated rotary bed shaft (1) to rotate. The two ends of the distributed turntable shaft (12) are pivotally inserted into the sealed cavity (21) of the distributed turntable, and the two ends extend out of the sealed cavity (21). One end extends out of the sealed cavity (21) of the distributed turntable and is connected to the turntable frequency conversion motor (28) for driving the distributed turntable shaft (12) to rotate.

3. The partitioned rotating bioreactor according to claim 1, characterized in that, The integrated rotating bed packing bed (4) is fixed on the integrated rotating bed shaft (1) and rotates synchronously with the integrated rotating bed shaft (1); the integrated rotating bed packing bed (4) includes a rotating bed packing area (27), which is filled with a combination of hydrophilic sponge packing and hydrophobic polyurethane packing, with a mass ratio of 1:

1.

4. The partitioned rotating bioreactor according to claim 1, characterized in that, The integrated rotating bed cavity (5) has an integrated rotating bed air outlet (10) on the side near the decentralized rotating plate processing unit, and the decentralized rotating plate cavity (21) has a decentralized rotating plate air inlet (13) on the side near the integrated rotating bed processing unit and a decentralized rotating plate air outlet (20) on the side away from the integrated rotating bed processing unit; the integrated rotating bed air outlet (10) and the decentralized rotating plate air inlet (13) are connected by a gas connection pipeline (24).

5. The partitioned rotating bioreactor according to claim 1, characterized in that, The distance between two adjacent algae-bacterial symbiotic loading turntables (14) is 1.5~2 cm; the surface of the algae-bacterial symbiotic loading turntables (14) is fixed with a porous fiber-based material with a thickness of 0.5~1 mm, which is used to load algae-bacterial symbiotic biofilm.

6. The partitioned rotating bioreactor according to claim 1, characterized in that, It also includes a spray liquid supply system and a visible light guiding system; among which: The spray supply system includes an integrated rotating bed spray system (9) and a dispersed rotating disc spray system (16). The integrated rotating bed spray system (9) and the dispersed rotating disc spray system (16) are located at the top of the integrated rotating bed cavity (5) and the dispersed rotating disc cavity (21), respectively, and are used to spray inorganic salt culture medium, nutrient solution or circulating liquid onto the integrated rotating bed packing bed (4) and the algae-bacterial symbiotic loading disc (14). The visible light import system is set in the sealed cavity (21) of the distributed turntable and includes a plant growth supplement light lamp. The plant growth supplement light lamp is set towards the algae-bacterial symbiotic loading turntable (14) and is used to provide the light required for photosynthesis of green algae in the algae-bacterial symbiotic biofilm.

7. The partitioned rotating bioreactor according to claim 1, characterized in that, The bottom of the integrated rotating bed sealed cavity (5) is provided with an integrated rotating bed bottom outlet (6), and the side wall is provided with an integrated rotating bed side outlet (7) for liquid discharge, liquid reflux and liquid level control; the bottom of the decentralized rotating disc sealed cavity (21) is provided with a decentralized rotating disc bottom outlet (17), and the side wall is provided with a decentralized rotating disc side outlet (18) for liquid discharge, liquid reflux and liquid level control. An integrated rotating bed water storage area (22) is provided above the integrated rotating bed spray system (9), and an integrated rotating bed water inlet pipe (8) is provided at the top of the integrated rotating bed water storage area (22); a decentralized rotating disc water storage area (23) is provided above the decentralized rotating disc spray system (16), and a decentralized rotating disc water inlet pipe (15) is provided at the top of the decentralized rotating disc water storage area (23).

8. A method for treating organic waste gas using a partitioned rotating bioreactor as described in any one of claims 1 to 7, characterized in that, The specific steps include the following: S1. The organic waste gas to be treated is introduced into the sealed cavity of the integrated rotating bed through the air inlet, so that it comes into contact with the composite bacteria loaded on the surface of the integrated rotating bed packing bed (4). At the same time, the integrated rotating bed spraying system (9) is turned on to spray the inorganic salt culture medium, nutrient solution or circulating liquid evenly onto the packing area (27) to achieve primary absorption, mass transfer and biodegradation of organic waste gas. S2. The residual organic waste gas after primary purification enters the sealed cavity of the integrated rotating bed through the gas connection pipeline (24) and comes into contact with the algae-bacterial symbiotic biofilm on the surface of the algae-bacterial symbiotic loading disc (14). At the same time, the decentralized rotating disc spraying system (16) is turned on to spray inorganic salt culture medium, nutrient solution or circulating liquid onto the surface of each algae-bacterial symbiotic loading disc (14) in a directional manner to maintain the activity and stability of the algae-bacterial symbiotic biofilm. Meanwhile, under light conditions and in low-speed purification biofilm formation mode, the functional bacteria in the algae-bacteria symbiotic biofilm further degrade the residual waste gas, while the microalgae use the CO2 generated during the waste gas mineralization process to assimilate and fix it through photosynthesis. S3. After the organic waste gas treatment is running stably, switch the decentralized rotary table treatment unit to the high-speed biomass harvesting mode, with a rotation speed of 100~500 r / min, so that the algae-bacterial symbiotic biofilm will detach from the surface of the algae-bacterial symbiotic rotating table under shearing action. After the algae and bacteria biomass is collected, algal lipids are obtained by centrifugation, drying, grinding and extraction with organic solvents.

9. The method according to claim 8, characterized in that, The rotational speed of the sealed cavity of the integrated rotating bed is 1~10 r / min, the spraying intensity of the spraying system is 0.25~0.75 m3 / (m²·h), and the liquid immersion rate of the rotating bed is 0~100%. The dispersed rotating disc has a low-speed purification film-forming mode and a high-speed biomass harvesting mode. In the low-speed purification film-forming mode, the rotational speed is 1~10 r / min, the spraying intensity of the spraying system is 0.25~0.75 m³ / (m²·h), and the liquid immersion rate is 0~100%.

10. The method according to claim 8, characterized in that, The organic waste gas includes one or more of cyclohexane, chlorobenzene, o-xylene, butyl acetate, and ethanol. In the treatment, the composite microbial community degrades the organic waste gas and generates CO2. The green algae in the algae-bacterial symbiotic biofilm are used to assimilate and fix CO2 and convert some carbon resources into algal lipids.

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