Three-dimensional z-shaped flow channel and x-shaped force balance type tissue cutting device

Abstract

The present application relates to the technical field of tissue cutting device, disclose a three-dimensional Z type runner, X type stress balance type tissue cutting device, including the shell, the shell inside is equipped with the framework runner, the framework runner is Z-shaped circuitous structure, the framework runner includes first channel, second channel and third channel which are vertically arranged and communicated with each other; the driving end of first displacement driving assembly is inserted into the inlet end of first channel, the driving end of second displacement driving assembly is inserted into the inlet end of second channel, the driving end of third displacement driving assembly is inserted into the inlet end of third channel; first channel is equipped with first cutting assembly, second channel is equipped with second cutting assembly, third channel is equipped with third cutting assembly, the tissue block in the present application is cut by corresponding cutting assembly in each channel, so that the tissue block experiences multi-directional, multi-batch shearing, greatly improves the cutting efficiency, and the whole process avoids open operation, meets the aseptic operation requirement.

Description

Technical Field

[0001] This invention relates to the field of tissue cutting device technology, specifically to a three-dimensional Z-shaped flow channel and X-shaped force-balanced tissue cutting device. Background Technology

[0002] Currently, in the fields of biomedical and laboratory research, the segmentation and processing of tissues (such as tumor tissue, skin, organ placental tissue, etc.) mainly relies on the following two methods: ① Manual cutting method: Operators manually cut tissue using a scalpel inside a clean bench. The drawbacks of this method are: the cutting effect is highly dependent on the operator's skill and condition, leading to poor consistency in sample processing and making standardization impossible; the operation is complex and time-consuming; furthermore, due to the open operation, tissue is exposed to ambient air for extended periods, making it highly susceptible to microbial contamination and difficult to meet aseptic requirements, especially for the preparation of cell therapy products under GMP standards.

[0003] ② Mechanical rotary shearing method: This method uses mechanical equipment with stirring blades or rotating cutters to shear tissue. The drawbacks of this method are: rotary shearing is a disordered cutting process, unable to achieve a stable size range, and prone to over-cutting, easily causing excessive mechanical damage to tissue cells, leading to high cell mortality; furthermore, the complex mechanical coupling structure between the drive motor and disposable consumables requires high sealing performance, increasing not only the cost of consumables but also the risk of fluid leakage and external contamination.

[0004] Therefore, how to provide a tissue cutting device that can achieve fully enclosed, sterile, and standardized operation while also being highly efficient and causing minimal cell damage is a technical problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0005] In view of this, the present invention provides a three-dimensional Z-shaped flow channel and X-shaped force-balanced tissue cutting device to solve the problems mentioned in the background art, and specifically discloses the following contents: A three-dimensional Z-shaped flow channel and X-shaped force-balanced tissue cutting device includes a housing, and a skeleton flow channel is provided inside the housing. The skeleton flow channel has a Z-shaped meandering structure and includes a first channel, a second channel and a third channel that are arranged vertically and interconnected in sequence. A first displacement driving assembly, a second displacement driving assembly, and a third displacement driving assembly are fixedly installed on the periphery of the housing to drive the tissue block sequentially through the first channel, the second channel, and the third channel. The driving end of the first displacement driving assembly extends into the first channel from the entrance end, the driving end of the second displacement driving assembly extends into the second channel from the entrance end, and the driving end of the third displacement driving assembly extends into the third channel from the entrance end. A first cutting assembly is provided in the first channel, a second cutting assembly is provided in the second channel, and a third cutting assembly is provided in the third channel. Each of the first, second, and third displacement driving assemblies has a clearance structure on its driving end to avoid the corresponding cutting assembly. The top of the housing has a sample inlet that connects to the top of the inlet end of the first channel, and the side of the housing has a sample outlet that connects to the outlet end of the third channel.

[0006] Furthermore, the first cutting assembly, the second cutting assembly, and the third cutting assembly each include two symmetrically arranged cutting units. Each cutting unit includes a blade holder, and the skeleton flow channel is provided with a slot adapted to the blade holder. The extended end of the blade holder is provided with a cutting blade.

[0007] Furthermore, the cutting blade is inclined to the flow direction of the skeleton flow channel.

[0008] Furthermore, the cutting blades on the two symmetrically arranged blade holders have opposite cutting edges and are arranged in an alternating pattern.

[0009] Furthermore, the first displacement driving component, the second displacement driving component, and the third displacement driving component are all pneumatic syringes, and the piston rod end of each pneumatic syringe is provided with a clearance groove that is compatible with the cutting blade.

[0010] Furthermore, a pre-cutting blade is fixedly provided on the top of the piston rod located in the first channel, which is used to cut larger tissue blocks into cross-sectional areas that can be accommodated by the skeleton flow channel.

[0011] Furthermore, a sterile filter is connected to the tail end of the pneumatic syringe for filtering the driving gas.

[0012] Furthermore, the inner wall of the skeleton flow channel is provided with a first guide groove that is adapted to the avoidance groove.

[0013] Furthermore, the insertion end face of the tool holder is flush with the inner wall of the skeleton flow channel, and the insertion end face of the tool holder is provided with a second guide groove that matches the clearance groove.

[0014] Furthermore, the sample inlet is connected to the top of the inlet end of the first channel via an inlet docking channel.

[0015] The beneficial effects of this invention are as follows: ① This invention utilizes a Z-shaped, meandering skeletal flow channel, through which tissue blocks sequentially pass through a first, second, and third channel. Within each channel, the tissue is subjected to static blade array cutting by corresponding cutting components (first, second, and third cutting components). This three-dimensional, mutually perpendicular cutting path allows the tissue to undergo precise physical shearing in multiple directions and batches within a short time. Compared to manual cutting or single-pass rotational shearing, this method achieves rapid and standardized cutting, significantly improving cutting efficiency. Furthermore, the entire process is driven by displacement-driven components (first, second, and third displacement-driven components), avoiding open-loop operation and meeting aseptic operation requirements.

[0016] ② This invention uses pneumatic drive (the first to third displacement drive components are all pneumatic syringes) to replace the traditional mechanical coupling transmission. The driving gas enters the tail of the pneumatic syringe through a sterile filter, pushing the piston rod, achieving complete physical isolation between the power source and the sample flow path. This ensures that the entire cutting process is carried out in a closed environment, effectively avoiding external contamination and meeting the GMP production standards for disposable consumables.

[0017] ③ In this invention, each cutting component includes two symmetrically arranged cutting units with opposite cutting blades arranged in an alternating pattern. When a tissue block is pushed through this X-shaped blade array, the lateral forces from the left and right (or top and bottom) sides cancel each other out, making the direction of the resultant force on the tissue completely consistent with the direction of advancement. This greatly avoids the "deflection" and "rubbing" phenomena that occur in traditional unidirectional oblique cutting, eliminates cell necrosis caused by compression, and thus results in a more regular geometric shape and higher cell viability of the cut tissue block.

[0018] ④ The number, spacing, and arrangement density of the cutting blades can be preset and changed according to the physical characteristics of different tissue samples and the size of the target culture, achieving a high degree of customization and being widely applicable to the processing needs of various types of tissues such as tumors, skin, and organs.

[0019] ⑤ The clearance groove at the front end of the piston rod cooperates with the first guide groove on the inner wall of the skeleton flow channel and the second guide groove on the end face of the blade holder, ensuring that the piston rod can be accurately guided during reciprocating motion. This avoids interference or damage to the blade due to deformation of the thin-walled clearance groove structure under stress, thus improving the reliability and manufacturability of the device. At the same time, the piston rod located in the first channel is equipped with a pre-cutting blade, which can pre-cut large tissue blocks into sizes that the flow channel can accommodate, improving the device's compatibility with samples of different sizes. Attached Figure Description

[0020] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0021] Figure 1 This is a perspective view of a three-dimensional Z-shaped flow channel and X-shaped force-balanced tissue cutting device according to the present invention.

[0022] Figure 2 This is an exploded view of a three-dimensional Z-shaped flow channel and X-shaped force-balanced tissue cutting device according to the present invention.

[0023] Figure 3 This is a schematic diagram of the internal structure of a three-dimensional Z-shaped flow channel and X-shaped force-balanced tissue cutting device according to the present invention.

[0024] Figure 4 This is a schematic diagram of the cutting unit in this invention.

[0025] In the figure: 1-Shell; 11-Sample inlet; 12-Sample outlet; 2-Inlet docking channel; 3-Skeleton flow channel; 31-First channel; 32-Second channel; 33-Third channel; 34-First guide groove; 41-First cutting assembly; 42-Second cutting assembly; 43-Third cutting assembly; 51-First displacement drive assembly; 52-Second displacement drive assembly; 53-Third displacement drive assembly; 61-Tool holder; 62-Cutting blade; 63-Second guide groove; 7-Avoidance groove; 8-Pre-cutting blade. Detailed Implementation

[0026] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0027] It should be noted that the terms "first," "second," etc., in the specification, claims, and accompanying drawings of this application are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such data can be used interchangeably where appropriate for the purposes of describing embodiments of this application herein. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion; for example, a process, method, system, product, or device that includes a series of steps or components is not necessarily limited to those explicitly listed, but may include other steps or components not explicitly listed or inherent to such processes, methods, products, or devices.

[0028] In this application, the terms "upper," "lower," "left," "right," "front," "rear," "top," "bottom," "inner," "outer," "middle," "vertical," "horizontal," "lateral," and "longitudinal" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. These terms are primarily for the purpose of better describing this application and its embodiments, and are not intended to limit the indicated device, element, or component to having a specific orientation, or to be constructed and operated in a specific orientation.

[0029] Furthermore, in addition to indicating location or positional relationship, some of the aforementioned terms may also have other meanings. For example, the term "above" may also be used in some cases to indicate a certain dependency or connection relationship. Those skilled in the art can understand the specific meaning of these terms in this application based on the specific circumstances.

[0030] Furthermore, the terms "installation," "setup," "equipped with," "connection," "linking," and "socketing" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral structure; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium, or an internal connection between two devices, components, or parts. Those skilled in the art can understand the specific meaning of these terms in this application based on the specific circumstances.

[0031] See appendix Figure 1-4 The present invention discloses a three-dimensional Z-shaped flow channel and X-shaped force-balanced tissue cutting device, including a shell 1, and a skeleton flow channel 3 inside the shell 1. The skeleton flow channel 3 has a Z-shaped meandering structure and includes a first channel 31, a second channel 32 and a third channel 33 arranged vertically and connected to each other in sequence. A first displacement driving assembly 51, a second displacement driving assembly 52, and a third displacement driving assembly 53 are fixedly installed around the periphery of the housing. These assemblies drive the tissue block sequentially through the first channel 31, the second channel 32, and the third channel 33. The driving end of the first displacement driving assembly 51 extends into the first channel 31, the driving end of the second displacement driving assembly 52 extends into the second channel 32, and the driving end of the third displacement driving assembly 53 extends into the third channel 33. A first cutting assembly 41 is provided in the first channel 31, a second cutting assembly 42 is provided in the second channel 32, and a third cutting assembly 43 is provided in the third channel 33. Each of the driving ends of the first displacement driving assembly 51, the second displacement driving assembly 52, and the third displacement driving assembly 53 has an avoidance structure to avoid the corresponding cutting assembly. The top of the housing 1 is provided with a sample inlet 11 and is connected to the top of the inlet end of the first channel 31. The side of the housing 1 is provided with a sample outlet 12 and is connected to the outlet end of the third channel 33.

[0032] In this embodiment, the avoidance structure can push the sample to the next channel and make it flush with the channel.

[0033] In this embodiment, the housing 1 adopts an upper and lower cover splicing structure and is encapsulated by ultrasonic welding.

[0034] In this embodiment, the sample outlet can be open or sealed. An open outlet facilitates manual processing of the cut sample, while a sealed outlet can be connected to a pipeline to achieve functions such as flushing and counting.

[0035] The first cutting assembly 41, the second cutting assembly 42, and the third cutting assembly 43 each include two symmetrically arranged cutting units. Each cutting unit includes a blade holder 61. The skeleton flow channel 3 is provided with a slot adapted to the blade holder 61, and the extended end of the blade holder 61 is provided with a cutting blade 62.

[0036] The cutting blade 62 is inclined to the flow direction of the skeleton flow channel 3 to reduce cutting resistance.

[0037] The cutting blades 62 on the two symmetrically arranged blade holders 61 have opposite cutting edges and are arranged alternately.

[0038] In this embodiment, the cutting blades 62 on the two symmetrically arranged blade holders 61 are X-shaped and intersecting.

[0039] The first displacement drive assembly 51, the second displacement drive assembly 52, and the third displacement drive assembly 53 are all pneumatic syringes, and the piston rod end of each pneumatic syringe is provided with a clearance groove 7 that is adapted to the cutting blade 62.

[0040] A pre-cutting blade 8 is fixedly provided on the top of the piston push rod located in the first channel 31, which is used to cut larger tissue blocks into cross-sectional areas that can be accommodated by the skeleton flow channel 3.

[0041] The pneumatic syringe is equipped with a sterile filter at the tail end to filter the driving gas, ensuring that the driving gas does not contaminate the inside of the syringe.

[0042] The inner wall of the skeleton flow channel 3 is provided with a first guide groove 34 that is adapted to the avoidance groove 7.

[0043] The insertion end face of the tool holder 61 is flush with the inner wall of the skeleton flow channel 3, and the insertion end face of the tool holder 61 is provided with a second guide groove 63 that is adapted to the clearance groove 7.

[0044] In this embodiment, the first guide groove 34 and the second guide groove 63 are provided to ensure that the clearance groove 7 on the piston rod will not be deformed by force and squeeze against the cutting blade 62.

[0045] The sample inlet 11 is connected to the top of the inlet end of the first channel 31 through the inlet docking channel 2.

[0046] This embodiment discloses a working method, which specifically includes the following steps: The tissue block is inserted through the sample inlet 11 and enters the first channel through the inlet docking channel 2. Under the action of the pre-cutting blade 8, the tissue block is cut into a cross-sectional area that can be accommodated by the skeleton flow channel 3. The piston pusher at the inlet of the first channel 31 operates, pushing the tissue block past the first cutting assembly 41. After being pushed and cut, the tissue block enters the second channel 32. The piston pusher at the inlet of the second channel 32 operates, pushing the tissue block past the second cutting assembly 42. After being pushed and cut, the tissue block enters the third channel 33. The piston pusher at the inlet of the third channel 33 operates, pushing the tissue block past the third cutting assembly 43. After being pushed and cut, the tissue block reaches the sample outlet 12.

[0047] The above are merely preferred embodiments of this application and are not intended to limit this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A three-dimensional Z-shaped flow channel and X-shaped force-balanced tissue cutting device, characterized in that, Includes a housing (1), and the housing (1) has a skeleton flow channel (3) inside. The skeleton flow channel (3) has a Z-shaped meandering structure. The skeleton flow channel (3) includes a first channel (31), a second channel (32) and a third channel (33) that are arranged vertically and connected to each other in sequence. The housing (1) is fixedly mounted with a first displacement driving component (51), a second displacement driving component (52), and a third displacement driving component (53) to drive the tissue block through the first channel (31), the second channel (32), and the third channel (33) in sequence. The driving end of the first displacement driving component (51) extends into the first channel (31), the driving end of the second displacement driving component (52) extends into the second channel (32), and the driving end of the third displacement driving component (53) extends into the third channel (33). A first cutting component (41) is provided in the first channel (31), a second cutting component (42) is provided in the second channel (32), and a third cutting component (43) is provided in the third channel (33). The driving ends of the first displacement driving component (51), the second displacement driving component (52), and the third displacement driving component (53) are all provided with a clearance structure to avoid the corresponding cutting component. The top of the housing (1) is provided with a sample inlet (11) and connected to the top of the inlet end of the first channel (31). The side of the housing (1) is provided with a sample outlet (12) and connected to the outlet end of the third channel (33).

2. The three-dimensional Z-shaped flow channel and X-shaped force-balanced tissue cutting device according to claim 1, characterized in that, The first cutting assembly (41), the second cutting assembly (42) and the third cutting assembly (43) each include two symmetrically arranged cutting units. The cutting unit includes a blade holder (61). The skeleton flow channel (3) is provided with a slot adapted to the blade holder (61). The extension end of the blade holder (61) is provided with a cutting blade (62).

3. The three-dimensional Z-shaped flow channel and X-shaped force-balanced tissue cutting device according to claim 2, characterized in that, The cutting blade (62) is inclined to the flow direction of the skeleton flow channel (3).

4. The three-dimensional Z-shaped flow channel and X-shaped force-balanced tissue cutting device according to claim 2, characterized in that, The cutting blades (62) on the two symmetrically arranged blade holders (61) have opposite cutting edges and are arranged in an alternating pattern.

5. The three-dimensional Z-shaped flow channel and X-shaped force-balanced tissue cutting device according to claim 2, characterized in that, The first displacement drive assembly (51), the second displacement drive assembly (52) and the third displacement drive assembly (53) are all pneumatic syringes, and the piston rod end of each pneumatic syringe is provided with a clearance groove (7) that is compatible with the cutting blade (62).

6. The three-dimensional Z-shaped flow channel and X-shaped force-balanced tissue cutting device according to claim 5, characterized in that, A pre-cutting blade (8) is fixedly provided on the top of the piston push rod located in the first channel (31) to cut larger tissue blocks into cross-sectional areas that can be accommodated by the skeleton flow channel (3).

7. The three-dimensional Z-shaped flow channel and X-shaped force-balanced tissue cutting device according to claim 5, characterized in that, The pneumatic syringe is connected to a sterile filter at the tail end for filtering the driving gas.

8. The three-dimensional Z-shaped flow channel and X-shaped force-balanced tissue cutting device according to claim 5, characterized in that, The inner wall of the skeleton flow channel (3) is provided with a first guide groove (34) that is adapted to the avoidance groove (7).

9. A three-dimensional Z-shaped flow channel and X-shaped force-balanced tissue cutting device according to claim 8, characterized in that, The insertion end face of the tool holder (61) is flush with the inner wall of the skeleton flow channel (3), and the insertion end face of the tool holder (61) is provided with a second guide groove (63) that is adapted to the clearance groove (7).

10. A three-dimensional Z-shaped flow channel and X-shaped force-balanced tissue cutting device according to claim 1, characterized in that, The sample inlet (11) is connected to the top of the inlet end of the first channel (31) through the inlet docking channel (2).

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