A biological fertilizer containing bacillus mucilaginosus HP-JZ2 and a preparation method thereof

Abstract

The application relates to the field of microorganisms, and particularly discloses a bio-fertilizer containing bacillus mucilaginosus HP-JZ2 and a preparation method thereof. The bio-fertilizer contains the following raw materials in parts by weight: grass carbon 20-40 parts, wheat bran 30-50 parts, sugar 2-5 parts, humus 15-25 parts, compound carrier 5-10 parts, bacillus mucilaginosus HP-JZ2 inoculum 0.42-0.62 parts, trace elements 0.4-0.8 parts and amino acids 1.2-1.6 parts. The preparation method is as follows: the grass carbon, the wheat bran and the humus are uniformly mixed, sterilized, cooled, and then the sugar, the amino acids, the compound carrier and the trace elements are added and uniformly mixed to obtain primary mixed material; the bacillus mucilaginosus HP-JZ2 inoculum and water are added to the primary mixed material and uniformly mixed to obtain mixed material; the mixed material is subjected to fermentation treatment, and then granulation and drying to obtain finished products; and the bio-fertilizer has the advantages of acid and salt resistance and growth promotion of crops.

Description

Technical Field

[0001] This application relates to the field of microbiology, and more specifically, to a bio-fertilizer containing Bacillus mucilaginosus HP-JZ2 and a method for preparing the same. Background Technology

[0002] Bacillus mucilaginosus, also known as silicate bacteria, is the source of biological agents that are widely used in agriculture.

[0003] Bacillus mucilaginosus can be used to prepare bio-fertilizers that can be applied to the soil. These bio-fertilizers can solubilize potassium, phosphorus, and nitrogen, promote crop and plant growth, improve crop resistance, and inhibit diseases. They can also improve soil structure, loosen the soil, and reduce compaction. However, Bacillus mucilaginosus growth is limited in acidic and saline environments, which affects the growth activity of the bacteria in the bio-fertilizer. Furthermore, the organic matter content in the soil also affects the activity of the bio-fertilizer.

[0004] Therefore, how to prepare a new type of bio-fertilizer that retains good activity and efficacy under acidic and saline conditions to promote crop growth is an urgent problem to be solved. Summary of the Invention

[0005] In order to prepare a new bio-fertilizer that still has good activity and effect under acidic and saline conditions and promotes crop growth, this application provides a bio-fertilizer containing Bacillus mucilaginosus HP-JZ2 and its preparation method.

[0006] In a first aspect, this application provides a bio-fertilizer containing Bacillus mucilaginosus HP-JZ2, employing the following technical solution: A bio-fertilizer containing Bacillus mucilaginosus HP-JZ2 comprises the following raw materials in parts by weight: 20-40 parts peat moss, 30-50 parts wheat bran, 2-5 parts sugar, 15-25 parts humus, 5-10 parts composite substrate, 0.42-0.62 parts Bacillus mucilaginosus HP-JZ2 inoculant, 0.4-0.8 parts trace elements, and 1.2-1.6 parts amino acids.

[0007] Preferably, the Bacillus mucilaginosus krassilnikov HP-JZ2 in the Bacillus mucilaginosus HP-JZ2 inoculum was deposited at the China Center for Type Culture Collection on November 14, 2022, with accession number CCTCC NO: M 20221792.

[0008] By adopting the above technical solution, the preserved Bacillus mucilaginosus HP-JZ2 exhibits good salt and acid resistance. The bacterial agent prepared by Bacillus mucilaginosus HP-JZ2 is combined with peat moss, humus, sugar, and wheat bran to ferment and produce microbial fertilizer. Bacillus mucilaginosus HP-JZ2 can utilize the humic acid and organic matter in peat moss, as well as the carbohydrates, proteins, and trace elements in wheat bran, and the nutrients in sugar and humus. The porous structure of peat moss facilitates the attachment and colonization of bacteria, providing nutrition while ensuring the growth and reproduction of bacteria under aerobic conditions. This improves the activity of the microbial fertilizer and increases the bacterial content. When the microbial fertilizer is added to the soil, it can further promote crop growth.

[0009] Under acidic or saline soil conditions, the combination of peat moss, composite carrier, and porous filler, while loading the microorganisms, allows the peat moss to neutralize the acidic environment and harmonize the soil environment. The composite carrier and porous filler can partially block saline and acidic substances, thereby further ensuring the activity of Bacillus mucilaginosus HP-JZ2 in the bio-fertilizer and ensuring its effect of promoting crop growth and regulating the soil environment.

[0010] Preferably, the sugar is composed of sucrose, molasses and trehalose in a mass ratio of 1:1-1.5:0.5-1.

[0011] By adopting the above technical solution, sucrose, molasses, and trehalose are combined. Sucrose serves as a readily available carbon source, promoting the initial reproduction of Bacillus mucilaginosus HP-JZ2. Meanwhile, the sucrose, glucose, and fructose in molasses continuously provide carbon sources during the reproduction of Bacillus mucilaginosus HP-JZ2, ensuring its activity during the fermentation of the microbial fertilizer. Trehalose can maintain its structure under high temperature and high salinity conditions. Adding trehalose to the bio-fertilizer can improve the survival rate and stress resistance of Bacillus mucilaginosus HP-JZ2 during subsequent storage and application. After the microbial fertilizer is added to the soil, it can still maintain good activity under acidic and saline conditions, promoting crop growth.

[0012] Preferably, the composite carrier is composed of corn cob powder and diatomaceous earth in a mass ratio of 1:0.5-1.

[0013] By adopting the above technical solution, corn cob powder and diatomaceous earth are combined. The corn cob powder, rich in cellulose, hemicellulose, and lignin, serves as a continuous carbon source, supporting the sustained growth and metabolism of *Bacillus mucilaginosus* HP-JZ2 during solid-state fermentation. Furthermore, the porous structure of corn cob powder facilitates oxygen diffusion and moisture retention, providing favorable fermentation conditions for aerobic fermentation of *Bacillus mucilaginosus* HP-JZ2 and ensuring its activity. Combined with the porous structure of diatomaceous earth, this further adsorbs and immobilizes the mucilaginous spores. Bacillus HP-JZ2 ensures both the bacterial content and activity in the bio-fertilizer and prevents its loss from the soil, allowing it to remain effective for a long time and promote crop growth. Meanwhile, fermented corn cobs increase soil organic matter content, and diatomaceous earth buffers acidity fluctuations, improves the soil environment, and blocks salt in saline soil conditions, slowing water evaporation. This further promotes the reproduction and growth of Bacillus HP-JZ2 in the soil, continuously boosting crop growth.

[0014] Preferably, the corn cob powder used as the loading material is prepared from corn cob powder, β-cyclodextrin solution and brassinolide solution in a mass ratio of 100:4-6:0.1-0.15.

[0015] By adopting the above technical solution, β-cyclodextrin can adsorb and stabilize brassinolide. The hydrophobic brassinolide molecules can be distributed inside β-cyclodextrin to form inclusion complexes. The hydrophilic outer surface of β-cyclodextrin, combined with the hydrophilicity of the corn cob surface, further attracts Bacillus mucilaginosus HP-JZ2 to attach to the surface of the corn cob. The porous structure of the corn cob provides space for colonization, and Bacillus mucilaginosus HP-JZ2 can utilize the nutrients in the corn cob to supply its growth and reproduction. When bio-fertilizer is added to the soil, β-cyclodextrin slowly releases brassinolide. Brassinolide can promote the secretion of organic acids and sugars by crop roots. The secretions can serve as an additional carbon source for Bacillus mucilaginosus HP-JZ2, further promoting its growth and reproduction. In addition, brassinolide enhances plant resistance (such as drought resistance, cold resistance, and salt resistance), improves rhizosphere ecological stability, and further promotes crop growth.

[0016] In acidic soils, the organic matter in corn cob powder can partially neutralize hydrogen ions and buffer pH fluctuations; β-cyclodextrin can maintain structural stability within a pH range of 4-8, ensuring the continuous release of brassinolide in acidic soils and promoting crop growth; while in high-salt environments, the porous structure of corn cob can adsorb sodium ions, mitigating osmotic pressure shocks. At the same time, brassinolide induces plants to synthesize antioxidant enzymes, reducing free radical accumulation, lowering oxidative damage, indirectly protecting the rhizosphere microbiota, and further ensuring crop growth.

[0017] Preferably, the diatomaceous earth carrier is prepared from diatomaceous earth, low DE value maltodextrin solution and zein in a mass ratio of 1:0.1-0.25:0.05-0.1.

[0018] By adopting the above technical solution, the porous structure of diatomaceous earth facilitates the loading of low DE value maltodextrin solution and zein. Using the low DE value maltodextrin solution as a bridge, a composite connection structure of zein and diatomaceous earth is constructed, increasing the specific surface area while ensuring porosity. After contact with the bacterial agent, it provides physical attachment sites for Bacillus mucilaginosus HP-JZ2. The porous structure facilitates gas flow, ensuring that the aerobic Bacillus mucilaginosus HP-JZ2 can grow and reproduce better. The low DE value maltodextrin solution is not easily soluble in room temperature water and does not dissolve easily during fermentation, ensuring that the structural state of the diatomaceous earth provides space for the growth and reproduction of Bacillus mucilaginosus HP-JZ2. Combined with the hydrophobicity of zein, it can prevent the diatomaceous earth from excessively absorbing water and affecting the growth of bacteria, thus ensuring the activity and growth and reproduction of bacteria in the bacterial fertilizer.

[0019] When microbial fertilizer is added to the soil, diatomaceous earth can loosen the soil. Combined with low DE value maltodextrin as a carbon source, its long-chain polymers can be slowly hydrolyzed to provide a continuous energy supply. Combined with the nutrients provided by corn gliadin, it further promotes the growth and reproduction of microorganisms in the soil, thereby promoting crop growth.

[0020] Preferably, the trace elements are composed of zinc sulfate, calcium sulfate and potassium dihydrogen phosphate in a mass ratio of 1:1-2:1-3.

[0021] By adopting the above technical solutions, zinc sulfate supplements zinc, promotes root development, and improves crop resistance; calcium sulfate contains calcium, which is an important component of cell walls and can improve soil permeability, reduce salinity, and ensure root respiration and growth; potassium dihydrogen phosphate supplements potassium, further promoting crop growth and improving crop resistance.

[0022] Preferably, the amino acid is composed of glutamic acid, glycine and lysine in a mass ratio of 1:0.5-1:0.5-1.

[0023] By adopting the above technical solutions, glutamic acid regulates nitrogen metabolism and salt tolerance, glycine chelates heavy metals and reduces toxicity, and lysine enhances drought resistance. Together, these three substances construct a multi-dimensional stress resistance barrier. Glutamic acid, glycine, and lysine can all promote chlorophyll production, forming a "triple greening" effect, which significantly improves the greenness and photosynthetic capacity of crops. Glutamic acid provides a nitrogen source framework, glycine activates micronutrients, and lysine promotes protein accumulation, achieving full-chain nutritional support from "absorption to utilization," thereby promoting crop growth.

[0024] Secondly, this application provides a method for preparing bio-fertilizer containing Bacillus mucilaginosus HP-JZ2, using the following technical solution: A method for preparing a bio-fertilizer containing Bacillus mucilaginosus HP-JZ2 includes the following steps: S1. After mixing peat moss, wheat bran, and humus evenly, sterilize and cool the mixture, then add sugar, amino acids, composite carrier, and trace elements and mix evenly to obtain the initial mixture. S2. Add Bacillus mucilaginosus HP-JZ2 bacterial agent and water to the initial mixture and stir evenly to obtain the mixture; S3. The mixture is fermented, then granulated and dried to obtain the finished product.

[0025] By adopting the above technical solutions, the prepared bio-fertilizer still has high bacterial activity and content in acidic or saline-alkali soils, which can promote crop growth.

[0026] Preferably, the fermentation treatment temperature is 34-38℃ and the fermentation time is 5-8 days.

[0027] By adopting the above technical solutions and limiting the fermentation temperature and time, the microbial content in the bio-fertilizer can be further increased and the microbial activity can be ensured. After the bio-fertilizer is added to the soil, it can promote crop growth.

[0028] In summary, this application has the following beneficial effects: 1. The preserved Bacillus mucilaginosus HP-JZ2 exhibits good salt and acid tolerance. Bacillus mucilaginosus HP-JZ2 can be used to prepare microbial fertilizer by fermentation with peat moss, humus, sugar, and wheat bran. HP-JZ2 can utilize the humic acid and organic matter in peat moss, as well as the carbohydrates, proteins, and trace elements in wheat bran, and the nutrients in sugar and humus. The porous structure of peat moss facilitates the attachment and colonization of the bacteria, providing nutrition while ensuring the bacteria grow and reproduce under aerobic conditions. This increases the activity and bacterial content of the microbial fertilizer. When added to the soil, the microbial fertilizer further promotes crop growth.

[0029] 2. Under acidic or saline soil conditions, the combination of peat moss, composite carrier, and porous filler, while loading the microorganisms, allows the peat moss to neutralize the acidic environment and harmonize the soil environment. The composite carrier and porous filler can partially block saline and acidic substances, thereby further ensuring the activity of Bacillus mucilaginosus HP-JZ2 in the bio-fertilizer and ensuring its effect of promoting crop growth and regulating the soil environment. Detailed Implementation

[0030] The present application will be further described in detail below with reference to the embodiments.

[0031] All of the following ingredients are commercially available.

[0032] Example of preparation of corn cob powder loaded with feed Preparation Example 1: Corn cob powder loaded with feed was prepared using the following method: Corn cobs are crushed through a 40-mesh sieve and dried with hot air to obtain corn cob powder; Weigh 5g of β-cyclodextrin and add 45g of deionized water. Stir at a constant temperature of 60℃ to obtain a β-cyclodextrin solution. Add 1000mg of brassinolide to 2000mL of anhydrous ethanol and dissolve by sonication to obtain a brassinolide solution. 0.12 g of sclerosin lactone solution was placed in 50 g of β-cyclodextrin solution and stirred at 50 °C for 3 h at a stirring speed of 200 r / min to obtain a composite solution. The composite liquid was evenly sprayed onto the surface of 100g of corn cob powder and air-dried at 45℃ to obtain the finished product; the finished product was passed through a 20-mesh sieve.

[0033] Preparation Example 2: The difference between this preparation example and Preparation Example 1 is that: Corn cobs are crushed through a 40-mesh sieve and dried with hot air to obtain corn cob powder; Weigh 5g of β-cyclodextrin and add 45g of deionized water. Stir at a constant temperature of 60℃ to obtain a β-cyclodextrin solution. Add 1000mg of brassinolide to 2000mL of anhydrous ethanol and dissolve by sonication to obtain a brassinolide solution. 0.1 g of sclerosin lactone solution was placed in 40 g of β-cyclodextrin solution and stirred at 50 °C for 3 h at a stirring speed of 200 r / min to obtain a composite solution. The composite liquid was evenly sprayed onto the surface of 100g of corn cob powder and air-dried at 45℃ to obtain the finished product; the finished product was passed through a 20-mesh sieve.

[0034] Preparation Example 3: The difference between this preparation example and Preparation Example 1 is that: Corn cobs are crushed through a 40-mesh sieve and dried with hot air to obtain corn cob powder; Weigh 5g of β-cyclodextrin and add 45g of deionized water. Stir at a constant temperature of 60℃ to obtain a β-cyclodextrin solution. Add 1000mg of brassinolide to 2000mL of anhydrous ethanol and dissolve by sonication to obtain a brassinolide solution. 0.15g of sclerosin lactone solution was placed in 60g of β-cyclodextrin solution and stirred at 50℃ for 3h at a stirring speed of 200r / min to obtain a composite solution. The composite liquid was evenly sprayed onto the surface of 100g of corn cob powder and air-dried at 45℃ to obtain the finished product; the finished product was passed through a 20-mesh sieve.

[0035] Example of preparation of diatomaceous earth with supporting material Preparation Example 4: The loaded diatomaceous earth was prepared using the following method: Dissolve the low DE value maltodextrin in warm water by stirring to obtain a 1% (w / w) low DE value maltodextrin solution. The temperature of the warm water is 65°C. 0.2 kg of low DE value maltodextrin solution was evenly sprayed onto the surface of 1 kg of diatomaceous earth. The diatomaceous earth was passed through a 60-mesh sieve. Then, 0.08 kg of zein was added at a rate of 100 g / min. During the addition process, the diatomaceous earth was continuously stirred at a speed of 80 r / min. After drying and dispersion, the finished diatomaceous earth was obtained and passed through a 40-mesh sieve.

[0036] Preparation Example 5: The difference between this preparation example and Preparation Example 4 is that: 0.1 kg of low DE value maltodextrin solution was uniformly sprayed onto the surface of 1 kg of diatomaceous earth. The diatomaceous earth was passed through a 60-mesh sieve. Then, 0.05 kg of zein was added at a rate of 100 g / min. During the addition process, the diatomaceous earth was continuously stirred at a speed of 80 r / min. After drying and dispersion, the finished diatomaceous earth was obtained and passed through a 40-mesh sieve.

[0037] Preparation Example 6: The difference between this preparation example and Preparation Example 4 is that: 0.25 kg of low DE value maltodextrin solution was evenly sprayed onto the surface of 1 kg of diatomaceous earth. The diatomaceous earth was passed through a 60-mesh sieve. Then, 0.1 kg of zein was added at a rate of 100 g / min. During the addition process, the diatomaceous earth was continuously stirred at a speed of 80 r / min. After drying and dispersion, the finished diatomaceous earth was obtained and passed through a 40-mesh sieve.

[0038] Preparation example of Bacillus mucilaginosus HP-JZ2 Preparation Example 7: Isolation and purification of Bacillus mucilaginosus HP-JZ2: Soil tested: Soil was collected from Laoshan, Qingdao. The sampling site was fruit and vegetable land that was more than 10 years old. The collected soil was mixed, crushed, and weeds, stones, tree roots and other debris were removed. The mixture was then placed in an ice bucket and stored at 4℃ for later use. The basic physicochemical properties of the soil tested are shown in Table 1. Table 1 Physicochemical properties of the tested soils

[0039] (2) Isolation medium: 15g beef extract, 10g peptone, 15g sodium chloride, 18g agar, add 900mL water, stir under 60℃ water bath conditions until all agar and other substances are dissolved, then add water to make up to 1L, adjust pH to 7.2, then sterilize at 121℃ for 20min, cool, and obtain isolation medium; Liquid culture medium: 10g sucrose, 1g ammonium chloride, 1g disodium hydrogen phosphate, 0.5g magnesium sulfate heptahydrate, 0.005g ferric chloride, 0.3g calcium carbonate, add 900mL water and mix well. Then add water to make up to 1L, pH 7.2, mix well, and then sterilize at 121℃ for 25min. Cool to obtain liquid culture medium. (3) Isolation and screening of Bacillus mucilaginosus HP-JZ2: Weigh 10g of the soil sample, add 90mL of sterile physiological saline, and shake for 1h at 30℃ and 120r / min to form a suspension. The suspension is then serially diluted to prepare 10 -1 10 -2 10 -3 10 -4 10 -5 10 -6 Diluted at multiples of 10 times, 10 μL of each dilution was evenly spread on the isolation medium, with three replicates for each dilution. The culture was carried out at 30°C for 2 days, and single colonies with good growth were selected to obtain the screening cells. (4) Purification of Bacillus mucilaginosus HP-JZ2: The selected bacterial cells were streaked onto the isolation medium using the streak method and cultured at 37°C for 24 hours. The above steps were repeated until the purified strain was obtained.

[0040] The purified strain was named Bacillus mucilaginosus HP-JZ2 and deposited at the China Center for Type Culture Collection on November 14, 2022, with accession number CCTCC NO: M 20221792. It was classified as Bacillus mucilaginosus krassilnikov HP-JZ2.

[0041] Identification of Bacillus mucilaginosus HP-JZ2: (1) Cell and colony morphology: After staining Bacillus mucilaginosus HP-JZ2, the cell morphology was observed under a microscope; The cells of Bacillus mucilaginosus HP-JZ2 are thick and long rods with rounded ends; the colonies on the culture medium are half-glass beads, smooth, moist and shiny, with neat and smooth edges, without forks or hairy protrusions.

[0042] Example of preparation of Bacillus mucilaginosus HP-JZ2 inoculum Preparation Example 8: Preparation of Bacillus mucilaginosus HP-JZ2 inoculum: Weigh out 1g of potassium aluminosilicate, 0.25g of sodium aluminosilicate, 8g of sucrose, 2g of disodium hydrogen phosphate, 0.005g of ferric chloride, 0.002g of calcium chloride, 0.45g of magnesium sulfate heptahydrate, and 20g of agar. Mix the agar with 900mL of water and stir in a 60℃ water bath until the agar is completely dissolved. Then add potassium aluminosilicate, sodium aluminosilicate, sucrose, disodium hydrogen phosphate, ferric chloride, calcium chloride, and magnesium sulfate heptahydrate and mix well. Add water to make up to 1L, pH 7.2, tilt to solidify, and then sterilize at 121℃ for 20min. Cool to obtain the slant culture medium. Weigh out 3g of glucose, 8g of molasses, 0.08g of chitin, 0.5g of dipotassium hydrogen phosphate, 0.5g of magnesium sulfate heptahydrate, 0.25g of sodium chloride, 0.8g of calcium carbonate, and 900mL of water. Mix well, then add water to make up to 1L. The pH is 7.2. Sterilize at 121℃ for 20min, cool, and the seed culture medium is obtained. Weigh out 7g of glucose, 4g of sucrose, 0.2g of yeast extract, 0.5g of ammonium sulfate, 1.2g of dipotassium hydrogen phosphate, 0.5g of calcium carbonate, 0.5g of ferric chloride, 0.2g of sodium chloride, and 0.2g of magnesium sulfate heptahydrate. Mix them with 900mL of water and stir well. Then add water to make up to 1L. The pH is 7.2. Sterilize at 121℃ for 20min and cool to obtain the fermentation medium. The Bacillus mucilaginosus HP-JZ2 strain from Preparation Example 7 was inoculated into an agar slant culture medium at an inoculation amount of 6%, and activated at 30°C for 2.5 days to obtain the activated strain. The activated strain was inoculated into seed culture medium at a rate of 6%, and cultured in a shake flask at 30°C for 12 hours at a shaker speed of 200 r / min to obtain the amplified strain. The amplified strain was inoculated into the fermentation medium at an inoculation amount of 7%, and fermented at 37°C for 48 hours with a shaking speed of 200 r / min. After centrifugation, the supernatant was removed, and the precipitate was washed three times with sterile physiological saline. Then, it was vacuum dried at 40°C and finally pulverized to obtain Bacillus mucilaginosus HP-JZ2 bacterial agent. Example

[0043] Example 1: A bio-fertilizer containing Bacillus mucilaginosus HP-JZ2: The formula consists of 30 kg of peat moss, 40 kg of wheat bran, 4 kg of sugar, 20 kg of humus, 8 kg of composite carrier material, 0.5 kg of Bacillus mucilaginosus HP-JZ2 inoculant, 0.6 kg of trace elements, and 1.4 kg of amino acids. The Bacillus mucilaginosus HP-JZ2 inoculant used is the one prepared in Preparation Example 8. The sugar consists of sucrose, molasses, and trehalose in a mass ratio of 1:1.2:0.8. The composite carrier material consists of corn cob powder (prepared in Preparation Example 1) and diatomaceous earth (prepared in Preparation Example 4) in a mass ratio of 1:0.8. The trace elements consist of zinc sulfate, calcium sulfate, and potassium dihydrogen phosphate in a mass ratio of 1:1.5:2. The amino acids consist of glutamic acid, glycine, and lysine in a mass ratio of 1:0.8:0.7. The preparation method is as follows: S1. After mixing peat moss, wheat bran, and humus evenly, sterilize at 121℃ for 20 minutes, cool to room temperature, add sugar, amino acids, composite carrier, porous filler, and trace elements, mix and stir evenly to obtain the initial mixture. S2. Add Bacillus mucilaginosus HP-JZ2 bacterial agent and water to the initial mixture, mix and stir evenly, control the moisture content to 50%, and obtain the mixture. S3. The mixture is fermented at 37℃ for 7 days, and then granulated and dried to obtain the finished product.

[0044] Example 2: The difference between this example and Example 1 is that: The formula consists of 20 kg of peat moss, 30 kg of wheat bran, 2 kg of sugar, 15 kg of humus, 5 kg of composite carrier, 0.42 kg of Bacillus mucilaginosus HP-JZ2 inoculant, 0.4 kg of trace elements, and 1.6 kg of amino acids. The Bacillus mucilaginosus HP-JZ2 inoculant used is the one prepared in Preparation Example 8. The sugar is composed of sucrose, molasses, and trehalose in a mass ratio of 1:1:0.5. The composite carrier is composed of corn cob powder (prepared in Preparation Example 2) and diatomaceous earth (prepared in Preparation Example 5) in a mass ratio of 1:0.5. The trace elements are composed of zinc sulfate, calcium sulfate, and potassium dihydrogen phosphate in a mass ratio of 1:1:1. The amino acids are composed of glutamic acid, glycine, and lysine in a mass ratio of 1:0.5:0.5. The preparation method is as follows: S1. After mixing peat moss, wheat bran, and humus evenly, sterilize at 121℃ for 20 minutes, cool to room temperature, add sugar, amino acids, composite carrier, porous filler, and trace elements, mix and stir evenly to obtain the initial mixture. S2. Add Bacillus mucilaginosus HP-JZ2 bacterial agent and water to the initial mixture, mix and stir evenly, control the moisture content to 50%, and obtain the mixture. S3. The mixture is fermented at 34℃ for 8 days, and then granulated and dried to obtain the finished product.

[0045] Example 3: The difference between this example and Example 1 is that: The formula consists of 40 kg of peat moss, 50 kg of wheat bran, 5 kg of sugar, 25 kg of humus, 10 kg of composite carrier, 0.62 kg of Bacillus mucilaginosus HP-JZ2 inoculant, 0.8 kg of trace elements, and 1.2 kg of amino acids. The Bacillus mucilaginosus HP-JZ2 inoculant used is the one prepared in Preparation Example 8. The sugar is composed of sucrose, molasses, and trehalose in a mass ratio of 1:1.5:1. The composite carrier is composed of corn cob powder (prepared in Preparation Example 3) and diatomaceous earth (prepared in Preparation Example 6) in a mass ratio of 1:1. The trace elements are composed of zinc sulfate, calcium sulfate, and potassium dihydrogen phosphate in a mass ratio of 1:3:3. The amino acids are composed of glutamic acid, glycine, and lysine in a mass ratio of 1:1:1. The preparation method is as follows: S1. After mixing peat moss, wheat bran, and humus evenly, sterilize at 121℃ for 20 minutes, cool to room temperature, add sugar, amino acids, composite carrier, porous filler, and trace elements, mix and stir evenly to obtain the initial mixture. S2. Add Bacillus mucilaginosus HP-JZ2 bacterial agent and water to the initial mixture, mix and stir evenly, control the moisture content to 50%, and obtain the mixture. S3. The mixture is fermented at 38℃ for 5 days, and then granulated and dried to obtain the finished product.

[0046] Example 4: The difference between this example and Example 1 is that: The corn cob powder used as the loading material was replaced with an equal mass of corn cob powder.

[0047] Example 5: The difference between this example and Example 1 is that: The diatomaceous earth carrier material was replaced with an equal mass of diatomaceous earth in the raw materials.

[0048] Performance testing 1. Bacterial activity detection The bacterial agents were prepared using the method described in Example 1, and the number of viable bacteria was detected. 1 kg of bacterial agent was added to 10 kg of sulfuric acid solution with pH 5, and after treatment for 24 h, the number of viable bacteria was detected and the data was recorded. 1 kg of bacterial agent was added to 10 kg of 5% sodium chloride solution, and after treatment for 24 h, the number of viable bacteria was detected and the data was recorded.

[0049] 2. Crop growth monitoring Bio-fertilizers were prepared using the methods described in Examples 1-5, respectively. The soil conditions were pH 5.8. Bio-fertilizer was applied to tomatoes before transplanting at a rate of 10 kg / mu. The plant height was recorded 45 days later (average of 100 plants was recorded) and designated as the experimental group. The plant height of tomatoes without bio-fertilizer was recorded 45 days after transplanting (average of 100 plants was recorded) and designated as the control group. The growth rate was calculated as (experimental group - control group) / control group × 100%.

[0050] The soil salt content was 3 g / kg. Bio-fertilizer was applied to tomatoes before transplanting at a rate of 10 kg / mu. The height of tomato plants was recorded 45 days later (average of 100 plants was recorded) and this was designated as the experimental group. The height of tomatoes without bio-fertilizer was recorded 45 days after transplanting (average of 100 plants was recorded) and this was designated as the control group. The growth rate was calculated as (experimental group - control group) / control group × 100%.

[0051] Table 2 Performance Test Table (In the table, " / " indicates that the corresponding embodiment did not test this item and there is no data)

[0052] As can be seen from Examples 1-3 and Table 2, the Bacillus mucilaginosus of this application has high activity. Under acidic or saline conditions, the activity and growth and reproduction rate of Bacillus mucilaginosus are not easily affected, and the prepared bio-fertilizer can promote crop growth.

[0053] Combining Examples 1 and 4-5 with Table 2, it can be seen that in Example 4, the corn cob powder was replaced with the same mass of corn cob powder as the carrier corn cob powder. Compared with Example 1, the growth rate of Example 4 was lower than that of Example 1. This indicates that the porous structure of corn cob provides space for colonization. β-cyclodextrin slowly releases brassinolide, which can promote the secretion of organic acids and sugars by crop roots. The secretions can serve as an additional carbon source for Bacillus mucilaginosus HP-JZ2, further promoting the growth and reproduction of Bacillus mucilaginosus HP-JZ2, improving the stability of the rhizosphere ecology, and further promoting crop growth.

[0054] In Example 5, the same mass of diatomaceous earth was used to replace the carrier diatomaceous earth in the raw materials. Compared with Example 1, the growth rate of Example 5 was lower than that of Example 1. This shows that diatomaceous earth can loosen the soil and maintain gas circulation. When combined with low DE value maltodextrin as a carbon source, its long-chain polymer can be slowly hydrolyzed to provide a continuous energy supply. Combined with the nutrients provided by zein, it further promotes the growth and reproduction of microorganisms in the soil, thereby promoting crop growth.

[0055] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.

Claims

1. A bio-fertilizer containing Bacillus mucilaginosus HP-JZ2, characterized in that, The ingredients comprise the following parts by weight: 20-40 parts peat moss, 30-50 parts wheat bran, 2-5 parts sugar, 15-25 parts humus, 5-10 parts composite substrate, 0.42-0.62 parts Bacillus mucilaginosus HP-JZ2 inoculant, 0.4-0.8 parts trace elements, and 1.2-1.6 parts amino acids.

2. The bio-fertilizer containing Bacillus mucilaginosus HP-JZ2 according to claim 1, characterized in that: The Bacillus mucilaginosus krassilnikov HP-JZ2 in the aforementioned Bacillus mucilaginosus HP-JZ2 inoculum was deposited at the China Center for Type Culture Collection on November 14, 2022, with accession number CCTCC NO: M20221792.

3. The bio-fertilizer containing Bacillus mucilaginosus HP-JZ2 according to claim 1, characterized in that, The sugar is composed of sucrose, molasses, and trehalose in a mass ratio of 1:1-1.5:0.5-1.

4. The bio-fertilizer containing Bacillus mucilaginosus HP-JZ2 according to claim 1, characterized in that, The composite carrier is composed of corn cob powder and diatomaceous earth in a mass ratio of 1:0.5-1.

5. The bio-fertilizer containing Bacillus mucilaginosus HP-JZ2 according to claim 4, characterized in that, The loading material, corn cob powder, is prepared from corn cob powder, β-cyclodextrin solution, and brassinolide solution in a mass ratio of 100:4-6:0.1-0.

15.

6. The bio-fertilizer containing Bacillus mucilaginosus HP-JZ2 according to claim 4, characterized in that, The diatomaceous earth carrier is prepared by mixing diatomaceous earth, low DE value maltodextrin solution and zein in a mass ratio of 1:0.1-0.25:0.05-0.

1.

7. The bio-fertilizer containing Bacillus mucilaginosus HP-JZ2 according to claim 1, characterized in that, The trace elements consist of zinc sulfate, calcium sulfate, and potassium dihydrogen phosphate in a mass ratio of 1:1-2:1-3.

8. The bio-fertilizer containing Bacillus mucilaginosus HP-JZ2 according to claim 1, characterized in that, The amino acid is composed of glutamic acid, glycine and lysine in a mass ratio of 1:0.5-1:0.5-1.

9. A method for preparing a bio-fertilizer containing Bacillus mucilaginosus HP-JZ2 according to any one of claims 1-8, characterized in that, Includes the following steps: S1. After mixing peat moss, wheat bran, and humus evenly, sterilize and cool the mixture, then add sugar, amino acids, composite carrier, and trace elements and mix evenly to obtain the initial mixture. S2. Add Bacillus mucilaginosus HP-JZ2 bacterial agent and water to the initial mixture and stir evenly to obtain the mixture; S3. The mixture is fermented, then granulated and dried to obtain the finished product.

10. The method for preparing a bio-fertilizer containing Bacillus mucilaginosus HP-JZ2 according to claim 9, characterized in that, The fermentation process is carried out at a temperature of 34-38℃ for 5-8 days.

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