Process for purifying dodecanedioic acid after fermentation and extraction equipment thereof

Abstract

The present application relates to the technical field of lauric acid purification, in particular to a lauric acid fermentation purity purification process and extraction equipment thereof; comprising the following steps: S1, high-temperature sterilization treatment is carried out on lauric acid fermentation liquor, the sterilization temperature is controlled at 90-100 DEG C, after sterilization is completed, it is cooled to 40-60 DEG C, the pH value of the system is adjusted to 7-11 by adding an alkaline reagent to the cooled fermentation liquor, after stirring and mixing uniformly, the clear fermentation filtrate is obtained by membrane filtration treatment; S2, the pH value of the fermentation filtrate obtained in step S1 is adjusted to 5-8 by adding an acidic reagent, the system temperature is maintained at 50 DEG C-100 DEG C, after the fermentation filtrate is loaded into the extraction equipment; the present application makes part of impurities dissolved by pH adjustment, and then removes insoluble impurities such as bacteria bodies, macromolecular impurities and the like by membrane filtration, so that the clear fermentation filtrate is obtained, which lays a pure foundation for subsequent purification, and solves the problem of incomplete impurity removal caused by non-targeted pretreatment in the traditional process.

Description

Technical Field

[0001] This invention relates to the field of lauryl ic acid purification technology, specifically to a process and extraction equipment for purifying lauryl ic acid after fermentation. Background Technology

[0002] Lauric acid, also known as dodecanoic acid, is an important long-chain dicarboxylic acid. It appears as a white powder with low water solubility and good thermal stability. It is widely used in the synthesis of nylon 612, nylon 1212, high-grade lubricants, plasticizers, and fragrances, and has high application value in the chemical, pharmaceutical, and fine chemical industries. Currently, industrially, lauric acid is mostly prepared by bio-fermentation. This method has mild reaction conditions and low production costs, but the fermentation broth has a complex composition, containing impurities such as bacteria, residual sugars, pigments, extraneous proteins, and inorganic salts. Purification processes are needed to remove these impurities to obtain high-purity products. Existing post-fermentation purification processes for lauric acid have significant drawbacks. They often employ single extraction or crystallization methods without targeted pretreatment of the fermentation broth, resulting in incomplete impurity removal and insufficient recovery of residual lauric acid in the aqueous phase. This makes it difficult to achieve high product purity. Furthermore, traditional processes are complex, energy-intensive, and involve significant lauric acid loss, failing to balance purification efficiency, product purity, and resource utilization, and thus failing to meet the demands of large-scale industrial production.

[0003] Extraction is the core step in the purification process of lauryl ic acid. The performance of the extraction equipment directly affects the purification efficiency and product quality. Most existing lauryl ic acid extraction equipment adopts a single discharge port design. After extraction, the organic phase and aqueous phase are allowed to separate into layers. The lower liquid phase must be discharged first, followed by the upper liquid phase. This makes it impossible to discharge the two phases simultaneously, resulting in low discharge efficiency. Furthermore, the upper and lower liquid phases share a single discharge port, which easily leads to cross-contamination and affects the purity of the product in subsequent refining processes. Summary of the Invention

[0004] To overcome the shortcomings of existing technologies, this invention proposes a purification process and extraction equipment for lauryl ic acid fermentation. This invention first sterilizes the lauryl ic acid fermentation broth at 90–100°C, then adjusts the pH to 7–11 using an alkaline reagent after cooling, and combines this with membrane filtration. This effectively kills the bacteria in the fermentation broth, destroys the bacterial structure, and dissolves the released impurities. Simultaneously, pH adjustment dissolves some impurities. Membrane filtration then rapidly removes insoluble impurities such as bacteria and large molecular proteins, resulting in a clear fermentation filtrate. This lays a pure foundation for subsequent purification and solves the problem of incomplete impurity removal caused by the lack of targeted pretreatment in traditional processes.

[0005] The technical solution adopted by this invention to solve its technical problem is: a process for purifying lauryl ic acid after fermentation, comprising the following steps: S1. Sterilize the lauryl iodide fermentation broth at high temperature, with the sterilization temperature controlled at 90-100℃. After sterilization, cool it to 40-60℃. Add an alkaline reagent to the cooled fermentation broth to adjust the pH value of the system to 7-11. Stir and mix evenly, and then filter through a membrane to obtain a clear fermentation filtrate. S2. Add an acidic reagent to the fermentation filtrate obtained in step S1 to adjust the pH of the system to 5-8, control the system temperature to be maintained at 50℃-100℃, load the fermentation filtrate into the extraction device, and then add a long-chain alcohol extractant to the fermentation filtrate. The amount of extractant is 0.01-0.5 of the volume of the fermentation filtrate. Stir and extract at a constant temperature for 0.5-3 hours. After extraction, let it stand to separate the layers and separate the organic phase loaded with lauryl iodide and the remaining aqueous phase. S3. Collect the organic phase separated in step S2 for the purification and recovery of lauryl diacid. Add decolorizing agent and chelating agent to the separated aqueous phase for decolorization, chelation and impurity removal treatment. After filtering to remove impurities, add acidic reagent again to adjust the pH value to 2-4.5 so that the residual lauryl diacid in the aqueous phase can be fully precipitated and crystallized. S4. The crystalline material precipitated in step S3 is subjected to centrifugation, washing with hot water at 70-100℃, and vacuum drying to finally obtain high-purity lauric acid product with a purity ≥99.5%.

[0006] A purification and extraction device for lauryl ic acid after fermentation is disclosed. This device is suitable for the aforementioned purification process of lauryl ic acid after fermentation. The device includes a frame and a tank inside the frame. The top of the tank has a feeding port and an upper discharge port. The feeding port is threadedly sealed with a plug. The bottom of the tank has a lower discharge port. Both the upper and lower discharge ports are connected to pipes. Two annular pusher discs are slidably and sealingly connected to the inner wall of the tank. Springs are fixed to the two pusher discs on opposite sides. An auxiliary motor is fixedly connected to the top and bottom walls of the tank. Receiving structures are provided on the top and bottom walls of the tank. The auxiliary motor output shaft extends into the receiving groove and is fixedly connected to a pull rope; the other end of the pull rope is connected to a pusher plate; the pusher plate has a circular rotating groove inside; a rotatable and sealed opening and closing plate is connected to the rotating groove; a square central groove runs through the center of the opening and closing plate; a square central rod is slidably and sealed to the central groove; the upper end of the central rod is driven by the main motor; a fan-shaped opening and closing groove runs through the opening and closing plate; a fan-shaped pushing groove runs through the pushing plate; the opening and closing groove is offset from or aligned with the pushing groove as the opening and closing plate rotates; valves are installed in both the upper and lower discharge ports.

[0007] Preferably, the grooves on the outer surface of the pusher disc are slidably and sealingly connected to the vertically arranged ribs on the inner wall of the tank.

[0008] Preferably, the pusher disc is provided with holes running through it from top to bottom; the top and bottom walls of the tank body are provided with air holes running outwards; the air holes are connected to the corresponding holes through elastic tubes.

[0009] Preferably, the top and bottom walls of the tank are provided with clearance grooves; one end of the spring is connected to the pusher plate, and the other end is connected to the inner wall of the clearance groove.

[0010] Preferably, the air hole passes through the clearance groove; one end of the elastic tube is connected to the pusher plate, and the other end is connected to the inner wall of the clearance groove and communicates with the air hole; the spring is sleeved on the outside of the corresponding elastic tube.

[0011] Preferably, the valve is composed of a valve plate, a valve stem, and a valve motor; the valve plate is rotatably and sealingly connected to the corresponding upper and lower discharge ports; the valve stem is centrally connected to the valve plate; and the valve stem is driven by the valve motor.

[0012] Preferably, the slot opening of the storage groove is fixedly connected to the perforated plate; the perforated plate is provided with a rope hole adapted to the outer diameter of the pull rope.

[0013] Preferably, the outer wall of the central rod is provided with a stirring groove; the stirring groove is rotatably connected to the stirring bar near its upper inner wall by a torsion spring; the specifications of the stirring bar are adapted to the stirring groove.

[0014] The beneficial effects of this invention are as follows: 1. This invention first sterilizes the lauryl iodide fermentation broth at 90-100℃, then adjusts the pH to 7-11 with an alkaline reagent after cooling, and combines this with membrane filtration. This effectively kills the bacteria in the fermentation broth, destroys the bacterial structure and releases impurities, and dissolves some impurities through pH adjustment. Finally, membrane filtration quickly removes insoluble impurities such as bacteria and large molecular proteins, resulting in a clear fermentation filtrate. This lays a pure foundation for subsequent purification and solves the problem of incomplete impurity removal caused by the lack of targeted pretreatment in traditional processes.

[0015] 2. This invention achieves simultaneous discharge of the organic phase and the aqueous phase by setting up two independent discharge ports at the top and bottom, and coordinating the pusher plate and the opening and closing plate for linkage control. This significantly shortens the discharge time, solves the problem of low discharge efficiency caused by the traditional single discharge port design, and improves the overall efficiency of the extraction process.

[0016] 3. The present invention achieves two-phase isolation through the staggered cooperation of the opening and closing disc and the pushing disc 3, and the upper and lower liquid phases are discharged through independent discharge ports, thereby avoiding cross-contamination between the two phases, ensuring the purity of the product in the subsequent refining process, and solving the pollution risk of traditional shared discharge ports. Attached Figure Description

[0017] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0018] Figure 1 This is a process flow diagram of the present invention; Figure 2 This is a perspective view of the device in this invention; Figure 3 This is a cross-sectional view of the device in this invention; Figure 4 yes Figure 3 Enlarged view of point A in the middle; Figure 5 yes Figure 3 Enlarged view of point B in the middle; Figure 6 This is a diagram of the internal structure of the tank in this invention; Figure 7 This is a perspective view of the pusher plate in this invention; Figure 8 This is a three-dimensional view of the opening and closing disc in this invention.

[0019] In the diagram: Frame 1, Tank 2, Feeding port 21, Upper discharge port 22, Screw plug 23, Lower discharge port 24, Collection trough 25, Perforated plate 251, Rope hole 252, Ribbed bar 26, Air hole 27, Elastic tube 28, Clearance groove 29, Pushing plate 3, Spring 31, Opening and closing groove 32, Opening and closing disc 33, Center groove 34, Pushing groove 35, Ribbed groove 36, Disc hole 37, Rotary groove 38, Auxiliary motor 4, Pull rope 41, Center rod 5, Main motor 51, Mixing tank 52, Mixing bar 53, Torsion spring 54, Valve 6, Valve plate 61, Valve stem 62, Valve motor 63. Detailed Implementation

[0020] To make the technical means, creative features, objectives and effects of this invention easier to understand, the invention will be further described below in conjunction with specific embodiments.

[0021] like Figures 1 to 8 As shown, the present invention includes the following embodiments: Example 1: A process for purifying lauryl ic acid after fermentation, comprising the following steps: S1. Sterilize the lauryl iodide fermentation broth at high temperature, with the sterilization temperature controlled at 90-100℃. After sterilization, cool it to 40-60℃. Add an alkaline reagent to the cooled fermentation broth to adjust the pH value of the system to 7-11. Stir and mix evenly, and then filter through a membrane to obtain a clear fermentation filtrate. S2. Add an acidic reagent to the fermentation filtrate obtained in step S1 to adjust the pH of the system to 5-8, control the system temperature to be maintained at 50℃-100℃, load the fermentation filtrate into the extraction device, and then add a long-chain alcohol extractant (long-chain alcohol or its isomers and esters as extractants) to the fermentation filtrate. The amount of extractant is 0.01-0.5 of the volume of the fermentation filtrate. Stir and extract at a constant temperature for 0.5-3 hours. After extraction, allow the mixture to stand and separate the layers, and separate the organic phase loaded with lauryl iodide and the remaining aqueous phase. S3. Collect the organic phase separated in step S2 for the purification and recovery of lauryl diacid. Add decolorizing agent and chelating agent to the separated aqueous phase for decolorization, chelation and impurity removal treatment. After filtering to remove impurities, add acidic reagent again to adjust the pH value to 2-4.5 so that the residual lauryl diacid in the aqueous phase can be fully precipitated and crystallized. S4. The crystalline material precipitated in step S3 is subjected to centrifugation, washing with hot water at 70-100℃, and vacuum drying to finally obtain high-purity lauric acid product with a purity ≥99.5%.

[0022] This invention first sterilizes the lauryl iodide fermentation broth at 90-100°C, then cools it and adjusts the pH to 7-11 with an alkaline reagent, followed by membrane filtration. This effectively kills the bacteria in the fermentation broth, destroys the bacterial structure and releases impurities, and dissolves some impurities through pH adjustment. Finally, membrane filtration quickly removes insoluble impurities such as bacteria and large molecular proteins, resulting in a clear fermentation filtrate. This lays a pure foundation for subsequent purification and solves the problem of incomplete impurity removal caused by the lack of targeted pretreatment in traditional processes. This invention achieves efficient extraction and separation of lauryl ic acid in the organic phase by adding an acidic reagent to the clarified fermentation filtrate to adjust the pH to 5-8, controlling the constant temperature condition of 50℃-100℃, adding a long-chain alcohol extractant and stirring at the constant temperature, controlling the amount of extractant to be 0.01-0.5% of the fermentation filtrate volume, and the extraction time to 0.5-3 hours. This significantly improves the extraction efficiency of lauryl ic acid and, compared with traditional single extraction methods, has stronger extraction targeting, better separation effect, and reduces the loss of lauryl ic acid during the extraction process. This invention involves adding decolorizing and chelating agents to the aqueous phase after extraction for decolorization and chelation to remove impurities. After filtration to remove residual impurities such as pigments and inorganic salts, the pH is adjusted to 2-4.5 to allow the residual lauric acid in the aqueous phase to fully crystallize. This fully recovers the residual lauric acid in the aqueous phase, avoids resource waste, solves the shortcomings of traditional processes in the insufficient recovery of residual lauric acid in the aqueous phase, and further improves the recovery rate of lauric acid. This invention effectively removes small amounts of impurities and moisture adhering to the crystal surface by sequentially centrifuging, washing with hot water at 70-100℃, and vacuum drying the precipitated crystalline material, ultimately obtaining a high-purity lauric acid product with a purity ≥99.5%, meeting the high purity requirements of industrial production. The present invention, through the above-mentioned step-by-step optimized purification process, has a simple and reasonable overall process, without the need for complicated and cumbersome operation procedures. Compared with the traditional process, it significantly reduces energy consumption, while taking into account purification efficiency, product purity and resource utilization, reducing the loss of lauryl ic acid, and solving the problem that the traditional process cannot meet multiple needs and is difficult to meet the requirements of industrial-scale production. It has significant industrial application value and economic benefits.

[0023] Example 2: A purification and extraction device for lauryl ic acid after fermentation. This device is applicable to the purification process for lauryl ic acid after fermentation as described in claim 1. The device includes a frame 1 and a tank 2 inside the frame 1. The top of the tank 2 is provided with a feeding port 21 and an upper discharge port 22. The feeding port 21 is threadedly sealed with a screw plug 23. The bottom of the tank 2 is provided with a lower discharge port 24. Both the upper discharge port 22 and the lower discharge port 24 are connected to pipes. The inner wall of the tank 2 is slidably sealed with two annular pusher discs 3. The two pusher discs 3 are fixedly connected to a spring 31 on opposite sides. An auxiliary motor 4 is fixedly connected inside the top and bottom walls of the tank 2. A receiving groove 25 is provided inside the top and bottom walls of the tank 2. The output shaft of the auxiliary motor 4 extends into the receiving groove 25 and is fixedly connected to the pull rope 41; the other end of the pull rope 41 is connected to the pusher plate 3; the pusher plate 3 has a circular rotating groove 38 inside; the opening and closing plate 33 is rotatably and sealed within the rotating groove 38; the opening and closing plate 33 has a square central groove 34 running through its center; the central groove 34 is slidably and sealed to a square central rod 5; the upper end of the central rod 5 is driven by the main motor 51; the opening and closing plate 33 has a fan-shaped opening and closing groove 32 running through its top and bottom; the pusher plate 3 has a fan-shaped pushing groove 35 running through its top and bottom; the opening and closing groove 32 is offset from or aligned with the pushing groove 35 as the opening and closing plate 33 rotates; valves 6 are provided in both the upper discharge port 22 and the lower discharge port 24.

[0024] In this embodiment, the groove 36 on the outer surface of the pusher plate 3 is slidably and sealed to the vertically arranged ribs 26 on the inner wall of the tank body 2.

[0025] In this embodiment, the pusher plate 3 is provided with a plate hole 37 running through it from top to bottom; the inner top wall and inner bottom wall of the tank body 2 are provided with an air hole 27 running through it from the outside; the air hole 27 is connected to the corresponding plate hole 37 through an elastic tube 28.

[0026] Before the equipment starts working, perform initialization preparations for each structure, ensuring that the valves 6 in the upper discharge port 22 and the lower discharge port 24 are both closed, the screw plug 23 is tightly connected to the feed port 21, both pusher discs 3 are in their initial positions, the spring 31 is in its natural extension and contraction state, and the auxiliary motor 4 and the main motor 51 are both in the stopped state. First, start the main motor 51. The output shaft of the main motor 51 drives the square center rod 5 to rotate. Since the center rod 5 and the square center groove 34 in the center of the opening and closing disc 33 are connected by a sliding seal, the rotation of the center rod 5 synchronously drives the opening and closing disc 33 to rotate in the rotating groove 38 of the pusher disc 3 until the fan-shaped opening and closing groove 32 on the opening and closing disc 33 and the fan-shaped pusher on the pusher disc 3 are aligned. When slots 35 are fully aligned, the main motor 51 stops operating, completing the connection preparation between the pusher plate 3 and the opening / closing plate 33. Next, the two auxiliary motors 4 are controlled to work synchronously. When the auxiliary motor 4 on the top wall inside the tank 2 starts, its output shaft rotates within the receiving slot 25 and releases the corresponding pull rope 41. When the auxiliary motor 4 on the bottom wall inside the tank 2 starts, its output shaft rotates within the receiving slot 25 and winds up the corresponding pull rope 41. During the winding process, the pull rope 41 pulls the lower pusher plate 3 downwards against the elastic force of the corresponding spring 31. Simultaneously, the upper pusher plate 3 moves downwards synchronously under the pushing force of its corresponding spring 31 until both pusher plates 3 have moved to their lowest possible positions within the tank 2. Then, both pusher plates stop operating. The auxiliary motor 4 operates to adjust the position of the pusher plate 3 before feeding. Then, the screw plug 23 on the feeding port 21 is unscrewed, and the fermentation filtrate is first fed into the inside of the tank 2. Next, the long-chain alcohol extractant is fed into the tank 2 according to the process requirements. After feeding, the screw plug 23 is tightened, and the fermentation filtrate and extractant in the tank 2 are mixed at a constant temperature. After mixing, the tank enters the settling and stratification stage. At this time, the liquid in the tank 2 can be interconnected through the pusher groove 35 of the pusher plate 3 and the opening / closing groove 32 of the opening / closing plate 33, ensuring that the liquid can smoothly achieve settling and stratification. Before settling and after mixing, the auxiliary motor 4 on the top wall of the tank 2 is started and the corresponding pull rope 41 is wound up, pulling the pusher plate 3 above. The spring 31 moves upward under its own force, and at the same time, the auxiliary motor 4 on the bottom wall of the tank 2 is started and the corresponding pull rope 41 is released. The pusher plate 3 below moves upward under the pushing force of its corresponding spring 31. According to the stratification requirements of the extraction system, the positions of the two pusher plates 3 are adjusted until the two pusher plates 3 move to the liquid stratification interface, that is, the junction of the upper organic phase and the lower aqueous phase is exactly between the two pusher plates 3. Then the auxiliary motor 4 stops working, the position of the pusher plate 3 remains unchanged, and it continues to stand until the liquid stratifies to form an upper liquid and a lower liquid. During the standing stratification process, the liquid can pass through the opening and closing groove 32 and the pusher groove 35, that is, the liquid can cross the pusher plate 3 and the opening and closing plate 33.After the liquid has completely separated, the main motor 51 is restarted, driving the central rod 5 and the opening / closing disc 33 to rotate, so that the opening / closing groove 32 on the opening / closing disc 33 is completely misaligned with the pushing groove 35 on the pushing disc 3. At this time, the two pushing discs 3, in conjunction with the tank body 2, completely separate the upper organic phase and the lower aqueous phase, preventing the two phases from mixing. Then, the valves 6 of the upper discharge port 22 and the lower discharge port 24 are opened, controlling the two auxiliary motors 4 to work again. The auxiliary motor 4 on the top wall inside the tank body 2 continuously winds up the pull rope 41, pulling the upper pushing disc 3 to continue moving upward and overcoming the elastic force of the spring 31. The auxiliary motor 4 on the bottom wall inside the tank body 2 continuously releases the pull rope 41, and the lower pushing disc 3 continues to move downward under the pushing force of the spring 31, so that the two pushing discs 3 move away from each other. Because the groove 36 on the outer surface of the pushing disc 3 is slidably sealed to the vertically set ribs 26 on the inner wall of the tank body 2, the pushing disc 3 can only slide up and down along the vertical direction of the tank body 2, and will not cause any mixing. Rotation ensures stability during the feeding process. Simultaneously, as the two feeding discs 3 move away from each other, external gas enters through the vents 27 on the top and bottom walls of the tank 2, is transported via the elastic tube 28 to the holes 37 of the feeding discs 3, and fills the space between the two feeding discs 3. This prevents negative pressure from hindering the movement of the feeding discs 3, allowing them to smoothly move away from each other. When the feeding discs 3 move away from each other, the upper feeding disc 3 squeezes the upper liquid, causing it to flow out and be collected along the upper outlet 22 and the connecting pipe. The lower feeding disc 3 squeezes the lower liquid, causing it to flow out and be collected along the lower outlet 24 and the connecting pipe, achieving synchronous discharge of both phases. After all liquid is completely discharged, the valves 6 of the upper outlet 22 and the lower outlet 24 are closed, and the motors are controlled to work in reverse, restoring the feeding discs 3, the opening and closing discs 33, and other structures to their initial positions, completing one extraction operation and allowing the next extraction cycle to begin. This invention achieves simultaneous discharge of the organic and aqueous phases by setting up two independent discharge ports, upper and lower, and coordinating the pusher plate 3 and the opening / closing plate 33 for linkage control. This significantly shortens the discharge time, solves the problem of low discharge efficiency caused by the traditional single discharge port design, and improves the overall efficiency of the extraction process. The invention also achieves phase isolation through the staggered arrangement of the opening / closing plate 33 and the pusher plate 3, with the upper and lower liquid phases discharged through independent discharge ports, thus avoiding cross-contamination and ensuring the purity of the product in subsequent refining processes, eliminating the contamination risks associated with traditional shared discharge ports. Furthermore, the invention utilizes the auxiliary motor 4, the pull rope 41, and the spring 31 to drive the pusher plate 3 up and down, thereby achieving flexible adjustment of the liquid stratification position to adapt to the stratification requirements of different extraction systems and improve the equipment's versatility. The invention offers several advantages: First, it ensures adaptability. Second, it establishes a sliding seal between the outer surface groove 36 of the pusher disc 3 and the inner wall ribs 26 of the tank body 2, restricting the pusher disc 3 to slide only vertically up and down, preventing rotational deviation, and ensuring the stability and reliability of the pushing, separating, and discharging processes. Third, it utilizes the interconnected design of the vent 27, elastic tube 28, and disc holes 37 to fill with external gas when the pusher discs 3 are far apart, thus preventing negative pressure between the two pusher discs 3, ensuring smooth movement of the pusher discs 3, reducing equipment operating losses, and extending equipment lifespan. Fourth, it uses the pusher disc 3 to expel liquid phase to assist in discharging, thereby reducing liquid phase residue in the tank body 2, increasing the collection rate of organic and aqueous phases, and further improving the recovery rate of lauryl ic acid in subsequent processes, reducing resource waste, and improving economic efficiency.

[0027] Example 3: Both the top and bottom walls of the tank 2 are provided with clearance grooves 29; one end of the spring 31 is connected to the pusher plate 3, and the other end is connected to the inner wall of the clearance groove 29.

[0028] In this embodiment, the air hole 27 passes through the clearance groove 29; one end of the elastic tube 28 is connected to the pusher plate 3, and the other end is connected to the inner wall of the clearance groove 29 and communicates with the air hole 27; the spring 31 is sleeved on the outside of the corresponding elastic tube 28.

[0029] During equipment operation, the core linkage is the separation and resetting phase of the pusher discs 3. After extraction is complete, liquid stratification is thorough, and phase separation is achieved, the two auxiliary motors 4 are activated, causing the two pusher discs 3 to begin moving away from each other. At this time, since the top and bottom walls of the tank 2 are equipped with clearance grooves 29, and one end of the spring 31 is connected to the pusher disc 3 and the other end to the inner wall of the clearance groove 29, the pusher discs 3 continuously overcome the elastic force of the spring 31 as they move away. As the pusher discs 3 move, the spring 31 is gradually compressed and eventually completely retracted into the clearance groove 29, forming effective clearance and preventing the spring 31 from being exposed and occupying space inside the tank 2. The vent 27 penetrates the clearance groove 29, and one end of the elastic tube 28 is connected to the pusher disc 3, and the other end is connected to the inner wall of the clearance groove 29 and communicates with the vent 27. The spring 31 is sleeved on the outside of the corresponding elastic tube 28. During the process of the pusher discs 3 moving away from each other... External gas enters the clearance groove 29 through the vent 27, and then is transported to the disc hole 37 of the pusher plate 3 via the elastic tube 28, filling the space between the two pusher plates 3. The spring 31, which is sleeved on the outside of the elastic tube 28, always provides protection for the elastic tube 28, preventing it from moving out of the spring 31 during equipment operation and preventing it from interfering with components such as the stirring mechanism inside the tank 2. When the pusher plate 3 moves to its limit position and the liquid is completely discharged, the motors are controlled to work in reverse, and the pusher plates 3 begin to move closer to each other and reset. At this time, the spring 31 stored in the clearance groove 29 gradually returns to its natural extension and contraction state, driving the pusher plate 3 to reset smoothly. The elastic tube 28 also resets synchronously under the protection of the spring 31 until the pusher plate 3 returns to its initial position, completing one structural linkage process and preparing for the next round of operation. The elastic tube 28 will also guide the spring 31 in the opposite direction. This invention provides clearance grooves 29 on the top and bottom walls of the tank 2, allowing the spring 31 to compress and retract into the clearance grooves 29 when the pusher discs 3 move away from each other. This effectively protects the spring 31 and reduces its space occupation within the tank 2, facilitating better discharge of residual liquid and materials and reducing residue levels. Furthermore, by sleeved on the outside of the elastic tube 28, the invention protects the elastic tube 28, preventing it from moving out of the spring 31 and interfering with components such as the stirring mechanism within the tank 2. This ensures stable equipment operation and reduces the failure rate. Simultaneously, the elastic tube 28 guides the spring 31 in the opposite direction, making its extension and retraction smoother.

[0030] Example 4: The valve 6 is composed of a valve plate 61, a valve stem 62 and a valve motor 63; the valve plate 61 is rotatably and sealingly connected to the corresponding upper discharge port 22 and lower discharge port 24; the valve stem 62 is connected through the center of the valve plate 61; the valve stem 62 is driven by the valve motor 63.

[0031] During the extraction process, when no material discharge is required, the valve motors 63 are controlled to operate. The valve motor 63 corresponding to the upper discharge port 22 drives the valve stem 62 to rotate, and the valve stem 62 simultaneously drives the valve plate 61 to rotate to a horizontal state. At this time, the valve plate 61 completely blocks the channel of the upper discharge port 22, thus closing the upper discharge port 22. The valve motor 63 corresponding to the lower discharge port 24 drives the valve stem 62 to rotate, and the valve stem 62 simultaneously drives the valve plate 61 to rotate to a horizontal state, thus closing the lower discharge port 24. When material discharge is required, the valve motors 63 are controlled to operate in the opposite direction, driving the corresponding valve stem 62 and valve plate 61 to rotate to a vertical state. The valve plates 61 of the upper discharge port 22 and the lower discharge port 24 no longer block their respective channels, thus realizing the synchronous opening of the upper discharge port 22 and the lower discharge port 24, meeting the operational requirements of separate material discharge of the two phases and equipment closure.

[0032] Example 5: The slot of the storage groove 25 is fixedly connected to the perforated plate 251; the perforated plate 251 is provided with a rope hole 252 that is adapted to the outer diameter of the pull rope 41.

[0033] During the process of the auxiliary motor 4 driving the pull rope 41 to wind or unwind, the pull rope 41 moves back and forth along the rope hole 252. The inner wall of the rope hole 252 is in close contact with the surface of the pull rope 41, which can scrape off the materials and impurities attached to the surface of the pull rope 41, prevent these substances from entering the storage groove 25 and affecting the winding and unwinding of the pull rope 41, and ensure the smooth operation of the pull rope 41.

[0034] Example 6: A stirring groove 52 is provided on the outer wall of the central rod 5; the stirring groove 52 is rotatably connected to the stirring bar 53 by a torsion spring 54 near the upper inner wall; the specifications of the stirring bar 53 are adapted to the stirring groove 52.

[0035] The height of the central groove 34 is greater than the movable gap between the stirring bar 53 and the end of the stirring tank 52. When it is necessary to stir and mix the fermentation filtrate and extractant in the tank 2, first control the two pusher plates 3 to move down to the limit position, and then control the main motor 51 to work. The main motor 51 drives the central rod 5 to rotate. During the rotation of the central rod 5, the end of the stirring bar 53 away from the torsion spring 54 is subjected to centrifugal force, which overcomes the elastic force of the torsion spring 54 and is thrown out from the stirring tank 52. It rotates synchronously with the central rod 5 to stir and mix the liquid in the tank 2. After the stirring is completed, the main motor 51 is stopped. The stirring bar 53 is returned to the stirring tank 52 under the reset action of the torsion spring 54. Since the stirring bar 53 and the stirring tank 52 are in movable sealing contact and their specifications are compatible, the two form a complete whole after being returned, ensuring that the surface of the central rod 5 is smooth, which makes it easy for the pusher plate 3 to drive the opening and closing plate 33 to slide up and down along the outer wall of the central rod 5.

[0036] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of the present invention is defined by the appended claims and their equivalents.

Claims

1. A process for purifying lauryl ic acid after fermentation, characterized in that, Includes the following steps: S1. Sterilize the lauryl iodide fermentation broth at high temperature, with the sterilization temperature controlled at 90-100℃. After sterilization, cool it to 40-60℃. Add an alkaline reagent to the cooled fermentation broth to adjust the pH value of the system to 7-11. Stir and mix evenly, and then filter through a membrane to obtain a clear fermentation filtrate. S2. Add an acidic reagent to the fermentation filtrate obtained in step S1 to adjust the pH of the system to 5-8, control the system temperature to be maintained at 50℃-100℃, load the fermentation filtrate into the extraction device, and then add a long-chain alcohol extractant to the fermentation filtrate. The amount of extractant is 0.01-0.5 of the volume of the fermentation filtrate. Stir and extract at a constant temperature for 0.5-3 hours. After extraction, let it stand to separate the layers and separate the organic phase loaded with lauryl iodide and the remaining aqueous phase. S3. Collect the organic phase separated in step S2 for the purification and recovery of lauryl diacid. Add decolorizing agent and chelating agent to the separated aqueous phase for decolorization, chelation and impurity removal treatment. After filtering to remove impurities, add acidic reagent again to adjust the pH value to 2-4.5 so that the residual lauryl diacid in the aqueous phase can be fully precipitated and crystallized. S4. The crystalline material precipitated in step S3 is subjected to centrifugation, washing with hot water at 70-100℃, and vacuum drying to finally obtain high-purity lauric acid product with a purity ≥99.5%.

2. A purification and extraction device for lauryl ic acid after fermentation, the device being applicable to the purification process for lauryl ic acid after fermentation as described in claim 1, characterized in that: The device includes a frame and a tank inside the frame; the top of the tank is provided with a feeding port and an upper discharge port; the feeding port is threadedly sealed with a plug; the bottom of the tank is provided with a lower discharge port; both the upper and lower discharge ports are connected to pipes; two annular pusher discs are slidably and sealed to the inner wall of the tank; the two pusher discs are fixedly connected to a spring on opposite sides; an auxiliary motor is fixedly connected to the inner top and bottom walls of the tank; a receiving groove is provided on the inner top and bottom walls of the tank; the output shaft of the auxiliary motor extends into the receiving groove and is fixedly connected to a pull... A rope; the other end of the rope is connected to a pusher disc; the pusher disc has a circular rotating groove inside; a rotatable and sealed opening and closing disc is connected to the rotating groove; a square central groove is provided through the center of the opening and closing disc; a square central rod is slidably and sealed to the central groove; the upper end of the central rod is driven by a main motor; a fan-shaped opening and closing groove is provided through the opening and closing disc; a fan-shaped pushing groove is provided through the pushing disc; the opening and closing groove is offset from or aligned with the pushing groove as the opening and closing disc rotates; valves are provided in both the upper and lower discharge ports.

3. The lauryl icing fermentation purification and extraction equipment according to claim 2, characterized in that: The grooves on the outer surface of the pusher plate are slidably and sealed to the vertically arranged ribs on the inner wall of the tank.

4. The lauryl icing fermentation purification and extraction equipment according to claim 2, characterized in that: The pusher disc has holes running through it from top to bottom; the top and bottom walls of the tank body have air holes running through them; the air holes are connected to the corresponding holes via elastic tubes.

5. The lauryl icing fermentation purification and extraction equipment according to claim 4, characterized in that: Both the top and bottom walls of the tank are provided with clearance grooves; one end of the spring is connected to the pusher plate, and the other end is connected to the inner wall of the clearance groove.

6. The lauryl icing fermentation purification and extraction equipment according to claim 5, characterized in that: The air hole passes through the clearance groove; one end of the elastic tube is connected to the pusher plate, and the other end is connected to the inner wall of the clearance groove and communicates with the air hole; the spring is sleeved on the outside of the corresponding elastic tube.

7. The lauryl icing fermentation purification and extraction equipment according to claim 2, characterized in that: The valve is composed of a valve plate, a valve stem, and a valve motor; the valve plate is rotatably and sealingly connected to the corresponding upper and lower discharge ports; the valve stem is connected through the center of the valve plate; the valve stem is driven by the valve motor.

8. The lauryl icing fermentation purification and extraction equipment according to claim 2, characterized in that: The slot opening of the storage groove is fixedly connected to the perforated plate; the perforated plate is provided with rope holes that are adapted to the outer diameter of the pull rope.

9. The lauryl icing fermentation purification and extraction equipment according to claim 2, characterized in that: The outer wall of the central rod is provided with a stirring groove; the stirring groove is rotatably connected to the stirring bar via a torsion spring near its upper inner wall; the specifications of the stirring bar are adapted to the stirring groove.

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