Preparation method of plant active essential oil, product and application thereof

Abstract

The application provides a preparation method of a plant active essential oil, a product and application thereof, and the preparation method comprises the following steps: mixing plant raw materials, enzymes, polyhydric alcohols and water, and performing an enzymolysis reaction to obtain an enzymolysis solution; mixing the enzymolysis solution, an entraining agent and an extractant, and performing subcritical extraction to obtain the plant active essential oil; the entraining agent comprises any one or a combination of at least two of capric caprylic triglyceride, grape seed oil, almond oil or mineral oil. The content of oil-soluble active ingredients in the plant raw materials is too low, and the specific entraining agent added in the subcritical extraction process can well carry the oil-soluble active ingredients, improve the content and stability of the active ingredients, and the prepared plant active essential oil has excellent moisturizing, soothing and firming effects.

Description

Technical Field

[0001] This invention belongs to the field of plant extract technology, specifically relating to a method for preparing plant active essential oil, its products, and applications. Background Technology

[0002] The main methods for extracting plant essential oils include steam distillation, cold pressing, and supercritical fluid extraction. However, these methods have some limitations in extracting plant essential oils. For example, while steam distillation is simple to operate, it takes a long time and may result in the loss of some volatile components. Although supercritical fluid extraction has high extraction efficiency, the equipment is expensive and the operation is complex, making it unsuitable for industrial production. Furthermore, existing methods often struggle to simultaneously preserve both the aroma and bioactive components of the essential oil during extraction.

[0003] Subcritical extraction is an extraction and separation technique that uses subcritical fluid as an extractant in a closed, oxygen-free, low-pressure vessel. Based on the principle of "like dissolves like" in organic compounds, the extraction material and the extractant undergo molecular diffusion during the soaking process, allowing the fat-soluble components in the bulk material to be transferred to the liquid extractant. The extractant and the target product are then separated through a process of reduced pressure evaporation, ultimately yielding the target product.

[0004] Therefore, it is of great significance to extract plant essential oils based on subcritical extraction to achieve a balance between the preservation of aroma and active ingredients. Summary of the Invention

[0005] To address the shortcomings of existing technologies, the present invention aims to provide a method for preparing plant-based active essential oils, as well as the products and applications thereof. This invention involves adding an entrainer during the subcritical extraction process of plant raw materials, which can effectively carry oil-soluble active ingredients, increasing the content and stability of the active ingredients. The resulting plant-based active essential oil exhibits excellent moisturizing, soothing, and firming effects.

[0006] To achieve this objective, the present invention employs the following technical solution: In a first aspect, the present invention provides a method for preparing a plant-based active essential oil, the method comprising: Plant raw materials, enzymes, polyols and water are mixed and subjected to enzymatic hydrolysis to obtain an enzymatic hydrolysate; the enzymatic hydrolysate, entrainer and extractant are mixed and subjected to subcritical extraction to obtain the plant active essential oil. The entrainer includes any one or a combination of at least two of the following: caprylic / capric triglyceride, grape seed oil, almond oil, or mineral oil.

[0007] The content of oil-soluble active ingredients in plant raw materials is too low. This invention adds a specific entrainer during the subcritical extraction process, which can effectively carry oil-soluble active ingredients, improve the content and stability of active ingredients, and produce plant active essence oils that have excellent moisturizing, soothing and firming effects.

[0008] Preferably, the plant material includes any one or a combination of at least two of saffron, rhodiola rosea, matsutake mushroom, or black ginger.

[0009] Preferably, the enzyme includes pectinase and / or cellulase.

[0010] Preferably, the mass ratio of the plant material to the enzyme is 100:(0.1~2).

[0011] The specific point values ​​in (0.1~2) can be 0.1, 0.2, 0.5, 0.7, 1, 1.3, 1.5, 1.8 or 2, etc.

[0012] Preferably, the enzymes include pectinase and cellulase.

[0013] Preferably, the mass ratio of pectinase to cellulase is (1~5):(1~5).

[0014] The specific point values ​​in the first (1~5) can be 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5 or 5, etc.

[0015] The specific point values ​​in the second (1~5) can be 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5 or 5, etc.

[0016] Preferably, the mass ratio of the plant material to the polyol is 100:(10~50).

[0017] The specific point values ​​in (10~50) can be 10, 15, 20, 25, 30, 35, 40, 45 or 50, etc.

[0018] Preferably, the mass ratio of the plant material to water is 100:(1~25).

[0019] The specific point values ​​in (1~25) can be 1, 2, 5, 7, 10, 13, 15, 18, 20, 22 or 25, etc.

[0020] Preferably, the temperature of the enzymatic hydrolysis reaction is 50~60℃, for example, 50℃, 51℃, 52℃, 53℃, 54℃, 55℃, 56℃, 57℃, 58℃, 59℃ or 60℃, etc.; the time is 5~10 h, for example, 5 h, 5.5 h, 6 h, 6.5 h, 7 h, 7.5 h, 8 h, 8.5 h, 9 h, 9.5 h or 10 h, etc.

[0021] Preferably, the polyol comprises any one or a combination of at least two of dipropylene glycol, butylene glycol, glycerol, or propylene glycol.

[0022] In the enzymatic hydrolysis reaction, the solvent and plant material are just moistened, with no significant liquid outflow, and the mixture remains dry. This invention uses a high concentration of polyol as the extraction medium, which facilitates the dissolution and release of active ingredients during the enzymatic hydrolysis reaction.

[0023] Preferably, the polyol includes dipropylene glycol and butanediol.

[0024] This invention preferably uses a combination of dipropylene glycol and butylene glycol for enzymatic hydrolysis. The two have a synergistic effect, which significantly improves the efficiency of the enzymatic hydrolysis reaction, thereby facilitating subsequent subcritical extraction and enhancing the efficacy of plant active essential oils.

[0025] Preferably, the mass ratio of dipropylene glycol to butanediol is 1:(1~5).

[0026] The specific point values ​​in (1~5) can be 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5 or 5, etc.

[0027] Preferably, the entrainer is caprylic / capric triglyceride.

[0028] This invention preferably uses caprylic / capric triglyceride as an entrainer, which can better carry oil-soluble active ingredients, increase the content and stability of active ingredients, and enhance the efficacy of plant active essential oils.

[0029] Preferably, the mass ratio of the plant material to the entrainer is 1:(1~5).

[0030] The specific point values ​​in (1~5) can be 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5 or 5, etc.

[0031] Preferably, the extractant comprises any one or a combination of at least two of propane, butane, tetrafluoroethane, or dimethyl ether.

[0032] Preferably, the ratio of the plant material to the extractant is 1 g:(3~6) mL.

[0033] The specific point values ​​in (3~6) can be 3, 3.2, 3.5, 3.7, 4, 4.3, 4.5, 4.8, 5, 5.5 or 6, etc.

[0034] Preferably, the subcritical extraction temperature is 40~50℃, for example, it can be 40℃, 41℃, 42℃, 43℃, 44℃, 45℃, 46℃, 47℃, 48℃, 49℃ or 50℃, etc.

[0035] Preferably, the subcritical extraction is performed 1 to 3 times, for example, once, twice or three times; each time is 30 min, 35 min, 40 min, 45 min, 50 min, 55 min or 60 min, etc.

[0036] Preferably, the subcritical extraction process further includes a purification step.

[0037] Preferably, the purification method is centrifugation.

[0038] Preferably, the centrifugation speed is 7000~14000 rpm, for example, it can be 7000 rpm, 8000 rpm, 9000 rpm, 10000 rpm, 11000 rpm, 12000 rpm, 13000 rpm or 14000 rpm, etc.

[0039] Secondly, the present invention provides plant active essential oils prepared by the preparation method described in the first aspect.

[0040] Preferably, the plant active essential oil is a single plant active essential oil or a compound plant active essential oil.

[0041] Preferably, the plant active essential oil is a compound plant active essential oil; the compound plant active essential oil is obtained by extracting the plant raw materials separately or by combining the extractions; the plant raw materials include a combination of at least two of saffron, rhodiola rosea, matsutake mushroom or black ginger.

[0042] Preferably, the extraction process includes: extracting plant raw materials separately using the preparation method described in the first aspect to obtain multiple plant active essential oils, and mixing the multiple plant active essential oils to obtain a composite plant active essential oil.

[0043] Preferably, the extraction process includes: first mixing the plant raw materials, and then extracting them using the preparation method described in the first aspect to obtain a compound plant active essential oil.

[0044] Preferably, the plant materials include saffron, rhodiola rosea, matsutake mushrooms, and black ginger.

[0045] This invention creatively discovers that plant-based active essential oils prepared from saffron, rhodiola rosea, matsutake mushrooms, and black ginger have a synergistic effect, working together to exert superior moisturizing, soothing, and firming effects.

[0046] Preferably, the mass ratio of saffron active essential oil, rhodiola active essential oil, matsutake active essential oil and black ginger active essential oil extracted separately is (1~10):(1~10):(1~10):(1~10).

[0047] The specific point value in the first (1~10) can be 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10, etc.

[0048] The specific point values ​​in the second (1~10) can be 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10, etc.

[0049] The specific point values ​​in the third (1~10) can be 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10, etc.

[0050] The specific point values ​​in the fourth (1~10) can be 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10, etc.

[0051] Preferably, in the combined extraction, the mass ratio of saffron, rhodiola rosea, matsutake mushroom and black ginger is (1~10):(1~10):(1~10):(1~10).

[0052] The specific point value in the first (1~10) can be 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10, etc.

[0053] The specific point values ​​in the second (1~10) can be 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10, etc.

[0054] The specific point values ​​in the third (1~10) can be 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10, etc.

[0055] The specific point values ​​in the fourth (1~10) can be 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10, etc.

[0056] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0057] Thirdly, the present invention provides the preparation method as described in the first aspect or the application of the plant active essential oil as described in the second aspect in the preparation of cosmetics, oral care products or functional foods.

[0058] Compared with the prior art, the present invention has the following beneficial effects: The content of oil-soluble active ingredients in plant raw materials is too low. This invention adds a specific entrainer during the subcritical extraction process, which can effectively carry oil-soluble active ingredients, improve the content and stability of active ingredients, and produce plant active essence oils that have excellent moisturizing, soothing and firming effects. Detailed Implementation

[0059] To further illustrate the technical means and effects of the present invention, the following describes the technical solution of the present invention in conjunction with preferred embodiments of the present invention. However, the present invention is not limited to the scope of the embodiments.

[0060] Where specific techniques or conditions are not specified in the examples, they shall be performed in accordance with the techniques or conditions described in the literature in this field, or in accordance with the product instructions. Reagents or instruments whose manufacturers are not specified are all conventional products that can be purchased through legitimate channels.

[0061] The sources of materials used in the following specific embodiments are as follows: Pectinase was purchased from Xiasheng, model SPE-006; cellulase was purchased from Xiasheng, model SPE-017L.

[0062] Example 1 This embodiment provides a saffron active essential oil, the preparation method of which includes: Saffron stamens were pulverized using an ultrafine pulverizer and passed through a 200-mesh sieve to prepare saffron powder. Pectinase and cellulase were mixed at a mass ratio of 1:3 to prepare a compound enzyme. Dipropylene glycol and butylene glycol were mixed at a mass ratio of 1:4 to prepare a compound polyol. 10 g of saffron powder, 0.05 g of the compound enzyme, 1.5 g of the compound polyol, and 0.3 g of water were mixed thoroughly and enzymatically hydrolyzed at 55°C for 6 h to obtain the enzymatic hydrolysate. 20 g of caprylic / capric triglyceride, 10 mL of butane, and 30 mL of tetrafluoroethane were added to the enzymatic hydrolysate, and the mixture was subjected to two subcritical extractions at 40°C for 60 min each. The mixture was centrifuged at 14000 rpm to remove the lower layer of water and the portion insoluble in the lipid phase, yielding saffron active essential oil.

[0063] Example 2 This embodiment provides a Rhodiola Rosea active essential oil, the preparation method of which includes: Rhodiola rosea root was pulverized using an ultrafine pulverizer and passed through a 200-mesh sieve to prepare Rhodiola rosea powder. Pectinase and cellulase were mixed at a mass ratio of 1:4 to prepare a compound enzyme. Dipropylene glycol and butylene glycol were mixed at a mass ratio of 1:3 to prepare a compound polyol. 10 g of Rhodiola rosea powder, 0.08 g of the compound enzyme, 1.8 g of the compound polyol, and 0.2 g of water were mixed evenly and enzymatically hydrolyzed at 50°C for 8 h to obtain the enzymatic hydrolysate. 30 g of caprylic / capric triglyceride, 10 mL of propane, and 30 mL of dimethyl ether were added to the enzymatic hydrolysate, and the mixture was subjected to two subcritical extractions at 45°C for 60 min each. The mixture was centrifuged at 12000 rpm to remove the lower layer of water and the portion insoluble in the lipid phase, yielding Rhodiola rosea active essential oil.

[0064] Example 3 This embodiment provides a matsutake mushroom active essence oil, the preparation method of which includes: Matsutake mushrooms were pulverized using an ultrafine pulverizer and passed through a 300-mesh sieve to prepare matsutake powder. Pectinase and cellulase were mixed at a mass ratio of 1:2 to prepare a compound enzyme. Dipropylene glycol and butylene glycol were mixed at a mass ratio of 1:2 to prepare a compound polyol. 10 g of matsutake powder, 0.03 g of the compound enzyme, 2 g of the compound polyol, and 0.2 g of water were mixed evenly and enzymatically hydrolyzed at 60℃ for 5 h to obtain the enzymatic hydrolysate. 30 g of caprylic / capric triglyceride, 10 mL of butane, and 30 mL of dimethyl ether were added to the enzymatic hydrolysate, and the mixture was subjected to two subcritical extractions at 42℃ for 30 min each. The mixture was centrifuged at 10000 rpm to remove the lower layer of water and the portion insoluble in the lipid phase, yielding matsutake active essential oil.

[0065] Example 4 This embodiment provides a black ginger active essential oil, the preparation method of which includes: Black ginger was pulverized using an ultrafine pulverizer and passed through a 150-mesh sieve to prepare black ginger powder. Pectinase and cellulase were mixed at a mass ratio of 1:5 to prepare a compound enzyme. Dipropylene glycol and butylene glycol were mixed at a mass ratio of 1:5 to prepare a compound polyol. 10 g of black ginger powder, 0.1 g of the compound enzyme, 1.5 g of the compound polyol, and 0.25 g of water were mixed evenly and enzymatically hydrolyzed at 50°C for 10 h to obtain the enzymatic hydrolysate. 20 g of caprylic / capric triglyceride, 10 mL of propane, and 50 mL of tetrafluoroethane were added to the enzymatic hydrolysate, and the mixture was subjected to two subcritical extractions at 50°C for 30 min each. The mixture was centrifuged at 12000 rpm to remove the lower layer of water and the portion insoluble in the lipid phase, yielding the active essential oil of black ginger.

[0066] Example 5 This embodiment provides a saffron active essential oil, which differs from Example 1 only in that caprylic / capric triglyceride is replaced with an equal amount of grape seed oil, while the other raw materials and steps remain unchanged.

[0067] Example 6 This embodiment provides a saffron active essential oil, which differs from Example 1 only in that caprylic / capric triglyceride is replaced with an equal amount of almond oil, while the other raw materials and steps remain unchanged.

[0068] Example 7 This embodiment provides a saffron active essential oil, which differs from Example 1 only in that: dipropylene glycol is not added, and the reduction is made up by butylene glycol, while the other raw materials and steps remain unchanged.

[0069] Example 8 This embodiment provides a saffron active essential oil, which differs from Example 1 only in that: butylene glycol is not added, and the reduction is made up by dipropylene glycol, while the other raw materials and steps remain unchanged.

[0070] Example 9 This embodiment provides a mulberry bark active essence oil, which differs from Embodiment 1 only in that: saffron powder is replaced with an equal amount of mulberry bark powder, while the other raw materials and steps remain unchanged.

[0071] Example 10 This embodiment provides a compound plant active essential oil, the preparation method of which includes: Example 1: Saffron active essential oil, Example 2: Rhodiola rosea active essential oil, Example 3: Matsutake active essential oil, and Example 4: Black ginger active essential oil were mixed evenly in a mass ratio of 1:1:1:1 to obtain the final product.

[0072] Example 11 This embodiment provides a compound plant active essential oil, which differs from Embodiment 10 only in that: saffron active essential oil is not added, and its reduced amount is proportionally allocated to Rhodiola rosea active essential oil, matsutake mushroom active essential oil, and black ginger active essential oil.

[0073] Example 12 This embodiment provides a compound plant active essential oil, which differs from Example 10 only in that: Rhodiola rosea active essential oil is not added, and its reduced amount is proportionally allocated to saffron active essential oil, matsutake active essential oil, and black ginger active essential oil.

[0074] Example 13 This embodiment provides a compound plant active essential oil, which differs from Example 10 only in that: no matsutake active essential oil is added, and its reduced amount is proportionally allocated to saffron active essential oil, rhodiola active essential oil, and black ginger active essential oil.

[0075] Example 14 This embodiment provides a compound plant active essential oil, which differs from Embodiment 10 only in that: no black ginger active essential oil is added, and its reduced amount is proportionally allocated to saffron active essential oil, rhodiola rosea active essential oil, and matsutake active essential oil.

[0076] Comparative Example 1 This comparative example provides a saffron active essential oil, which differs from Example 1 only in that caprylic / capric triglyceride was not added during the subcritical extraction process, while the other raw materials and steps remain unchanged.

[0077] Comparative Example 2 This comparative example provides a saffron active essential oil, the preparation method of which includes: Saffron stamens were pulverized using an ultrafine pulverizer and passed through a 200-mesh sieve to prepare saffron powder. Pectinase and cellulase were mixed at a mass ratio of 1:3 to prepare a compound enzyme. Dipropylene glycol and butylene glycol were mixed at a mass ratio of 1:4 to prepare a compound polyol. 10 g of saffron powder, 0.05 g of the compound enzyme, 1.5 g of the compound polyol, and 0.3 g of water were mixed evenly and subjected to enzymatic hydrolysis at 55℃ for 6 h to obtain the hydrolysate. 1 g of 50 vol% ethanol aqueous solution was added to the hydrolysate, and supercritical CO2 extraction was performed at an extraction temperature of 40℃, an extraction pressure of 30 MPa, a CO2 flow rate of 10 kg / h, and an extraction time of 120 min to obtain saffron active essential oil.

[0078] Test Example 1 Wild-type AB strain zebrafish were used as the experimental system. Four-day-old zebrafish were randomly selected and placed in 6-well plates, with 30 fish per well. The test was performed according to the "T / ZHCA 015-2022 Evaluation of Firming Efficacy in Cosmetics: Relative Expression Level of Elastin Gene in Juvenile Zebrafish". The experimental group consisted of 0.1% (v / v) of the essential oils from Examples 1-14 or Comparative Examples 1-2, while the control group consisted of no added composition. Each well contained 3 mL. Each group was biologically replicated three times, incubated at 28°C in the dark for 24 hours, and the relative expression level of the eln1 gene in zebrafish was detected.

[0079] The test results are shown in Table 1. Examples 1-4 and Comparative Examples 1-2 demonstrate that the addition of a specific entrainer during subcritical extraction in this invention effectively carries oil-soluble active ingredients, increasing their content and stability, resulting in superior firming effects, even surpassing conventional supercritical CO2 extraction. Examples 5-6 show that the type of entrainer further affects the extraction effect, with caprylic / capric triglyceride showing the best effect. Examples 7-8 show that dipropylene glycol and butylene glycol have a synergistic effect in the enzymatic hydrolysis reaction, significantly improving the efficiency of the enzymatic hydrolysis reaction, thus facilitating subsequent subcritical extraction. Example 9 shows that the method of this invention has better extraction effects for specific plant materials. Examples 10 and 11-14 show that saffron, rhodiola rosea, matsutake mushroom, and black ginger have a synergistic effect in the plant active essential oil prepared by the specific method of this invention, resulting in superior firming effects.

[0080] Table 1 Test Example 2 Zebrafish with 2 dpf transgenic green fluorescently labeled neutrophils were randomly selected and placed in 6-well plates, 15 fish per group. The test was conducted according to the "T / ZHCA 016-2022 Evaluation of Soothing Efficacy of Cosmetics: Neutrophil Inhibition Rate Method in Juvenile Zebrafish". The experimental groups used 0.1% (v / v) of the essential oils from Examples 1-14 or Comparative Examples 1-2. A model control group was treated with SLS but received no sample, while a blank control group was formed by normal culture without SLS treatment and no sample. Each well contained 3 mL. Ten zebrafish from each test group were randomly selected and photographed under a fluorescence microscope. Advanced image processing software was used to analyze and collect data, count the number of neutrophils in the zebrafish, calculate the neutrophil inhibition rate, and evaluate the soothing efficacy.

[0081] The test results are shown in Table 2: Examples 1-4 and Comparative Examples 1-2 demonstrate that the addition of a specific entrainer during subcritical extraction in this invention effectively carries oil-soluble active ingredients, increasing their content and stability, and providing excellent soothing effects, even surpassing conventional supercritical CO2 extraction. Examples 5-6 show that the type of entrainer further affects the extraction effect, with caprylic / capric triglyceride showing the best effect. Examples 7-8 show that dipropylene glycol and butylene glycol have a synergistic effect in the enzymatic hydrolysis reaction, significantly improving the efficiency of the enzymatic hydrolysis reaction, thus facilitating subsequent subcritical extraction. Example 9 shows that the method of this invention has better extraction effects for specific plant materials. Examples 10 and 11-14 show that saffron, rhodiola rosea, matsutake mushroom, and black ginger have a synergistic effect in the plant active essential oil prepared by the specific method of this invention, providing superior soothing effects.

[0082] Table 2 Test Example 3 Safety testing was conducted using a 24-hour closed patch test. 0.020 mL of the plant active essential oils provided in Examples 1-14 were measured into the patch tester, and a blank control group (without any added substances) was set up. In accordance with the "Cosmetic Safety Technical Specifications (2015 Edition)," the patch tester was applied to selected locations on the hands of the subjects (a total of 31 subjects, 5 males and 26 females, aged 22-50 years, meeting the subject inclusion criteria) using hypoallergenic adhesive tape.

[0083] The results showed that in the closed patch test on human skin, all subjects showed negative reactions at 24h and 48h, and there was no irritation or erythema, indicating that the product involved in the present invention has no adverse reactions on the human body and is highly safe.

[0084] This invention illustrates a method for preparing a plant-based active essential oil, its product, and its application through the above embodiments. However, this invention is not limited to the above embodiments, meaning that it does not necessarily depend on the above embodiments for implementation. Those skilled in the art should understand that any improvements to this invention, equivalent substitutions of raw materials, additions of auxiliary components, and selection of specific methods, etc., all fall within the protection and disclosure scope of this invention.

[0085] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.

[0086] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not describe the various possible combinations separately.

Claims

1. A method for preparing a plant-based active essential oil, characterized in that, The preparation method includes: Plant raw materials, enzymes, polyols and water are mixed and subjected to enzymatic hydrolysis to obtain an enzymatic hydrolysate; the enzymatic hydrolysate, entrainer and extractant are mixed and subjected to subcritical extraction to obtain the plant active essential oil. The entrainer includes any one or a combination of at least two of the following: caprylic / capric triglyceride, grape seed oil, almond oil, or mineral oil.

2. The preparation method according to claim 1, characterized in that, The plant materials include any one or a combination of at least two of the following: saffron, rhodiola, matsutake mushroom, or black ginger. Preferably, the enzyme includes pectinase and / or cellulase; Preferably, the mass ratio of the plant material to the enzyme is 100:(0.1~2); Preferably, the enzymes include pectinase and cellulase; Preferably, the mass ratio of pectinase to cellulase is (1~5):(1~5); Preferably, the mass ratio of the plant material to the polyol is 100:(10~50); Preferably, the mass ratio of the plant material to water is 100:(1~25); Preferably, the enzymatic hydrolysis reaction is carried out at a temperature of 50-60°C for 5-10 hours.

3. The preparation method according to claim 1 or 2, characterized in that, The polyol includes any one or a combination of at least two of dipropylene glycol, butylene glycol, glycerol, or propylene glycol. Preferably, the polyol comprises dipropylene glycol and butanediol; Preferably, the mass ratio of dipropylene glycol to butanediol is 1:(1~5).

4. The preparation method according to any one of claims 1 to 3, characterized in that, The entrainer is caprylic / capric triglyceride; Preferably, the mass ratio of the plant material to the entrainer is 1:(1~5).

5. The preparation method according to any one of claims 1 to 4, characterized in that, The extractant includes any one or a combination of at least two of propane, butane, tetrafluoroethane or dimethyl ether; Preferably, the ratio of the plant material to the extractant is 1 g:(3~6) mL; Preferably, the subcritical extraction temperature is 40~50℃; Preferably, the subcritical extraction is performed 1 to 3 times, with each extraction lasting 30 to 60 minutes. Preferably, the subcritical extraction process further includes a purification step. Preferably, the purification method is centrifugation; Preferably, the centrifugation speed is 7000~14000 rpm.

6. The plant active essential oil prepared by the preparation method according to any one of claims 1 to 5.

7. The plant-based active essential oil according to claim 6, characterized in that, The plant-based active essential oil is either a single plant-based active essential oil or a compound plant-based active essential oil.

8. The plant-based active essential oil according to claim 7, characterized in that, The plant active essential oil is a compound plant active essential oil; the plant raw materials are extracted separately or in combination to obtain the compound plant active essential oil; the plant raw materials include a combination of at least two of saffron, rhodiola rosea, matsutake mushroom or black ginger; The extraction process includes: extracting plant raw materials separately using the preparation method described in any one of claims 1 to 5 to obtain a variety of plant active essential oils, and mixing the various plant active essential oils to obtain a composite plant active essential oil; The extraction process includes: first mixing the plant raw materials, and then extracting them using the preparation method described in any one of claims 1 to 5 to obtain a composite plant active essential oil.

9. The plant-based active essential oil according to claim 8, characterized in that, The plant ingredients include saffron, rhodiola rosea, matsutake mushrooms, and black ginger; Preferably, the mass ratio of saffron active essential oil, rhodiola active essential oil, matsutake active essential oil and black ginger active essential oil extracted separately is (1~10):(1~10):(1~10):(1~10); Preferably, in the combined extraction, the mass ratio of saffron, rhodiola rosea, matsutake mushroom and black ginger is (1~10):(1~10):(1~10):(1~10).

10. The preparation method according to any one of claims 1 to 5 or the application of the plant active essential oil according to any one of claims 6 to 9 in the preparation of cosmetics, oral care products or functional foods.

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