A device and method for synergistic cultivation of agarwood-forming dual bacteria

Abstract

The application discloses a linaloe dual-bacteria synergic culture device and method, and relates to the technical field of bacteria culture.The linaloe dual-bacteria synergic culture device comprises a culture box, a control panel, a conveying pump, a warning light, a production mechanism, a quantitative mechanism and a linkage mechanism; the production mechanism is provided with an independent bacteria liquid storage tank, and a pressing plate and a spring are used to realize sealed storage of dual bacteria liquid and prevent cross contamination; the quantitative mechanism adopts a servo motor, a worm and a worm wheel and a quantitative plate to accurately control the proportion of bacteria liquid pouring; and the linkage mechanism realizes synchronous action of two sets of material pouring systems through an electric push rod, a linkage sleeve and a sliding rod.The application solves the problems of single bacteria inoculation, ratio and timing difficult to control and unstable quality in traditional linaloe technology, realizes independent storage, accurate quantitative and synchronous pouring of dual bacteria liquid, improves linaloe efficiency and quality stability, and is suitable for large-scale and standardized production of linaloe.

Description

Technical Field

[0001] This invention relates to the field of microbial culture, specifically to a device and method for the synergistic cultivation of two microorganisms that induce agarwood formation. Background Technology

[0002] The co-culture of agarwood-forming fungi refers to the technique of using two specific fungi to act on agarwood trees to induce efficient and high-quality resin formation. This method simulates the complex process of multi-species co-infection in nature by precisely controlling the ratio and application of the two fungal solutions, stimulating the agarwood tree to activate its defense mechanism and secrete resin.

[0003] In existing technologies, the artificial formation of agarwood largely relies on physical damage stimulation, such as drilling, burning, or the traditional "opening the resin gate" method. These methods create wounds on the tree trunk, relying on the tree's self-repair and resin secretion, resulting in problems such as long resin formation cycles, low yields, and uneven quality. Subsequent fungal infection methods often employ single-species inoculation via artificial drip, relying on experience to determine the proportion and timing of fungal solution application. This makes it difficult to precisely control the synergistic effects of two fungi, easily leading to problems such as unbalanced fungal strain ratios, asynchronous inoculation, and cross-contamination. Consequently, the resin formation efficiency is low, the quality is unstable, and it is difficult to meet the needs of standardized, large-scale production.

[0004] To address the aforementioned deficiencies, this invention provides a device and method for the synergistic cultivation of two agarwood-forming bacteria, enabling independent storage, precise quantification, and simultaneous dispensing of the two bacterial solutions, thereby improving the efficiency and quality stability of agarwood formation. Summary of the Invention

[0005] The purpose of this invention is to solve the problems of single-strain inoculation, uncontrollable feeding ratio and timing, easy cross-contamination, long resin formation cycle and unstable quality in existing agarwood inoculation technology. It provides a dual-strain co-cultivation equipment and method for agarwood inoculation, so as to achieve precision, synchronization and standardization of dual-strain co-cultivation.

[0006] The technical solution adopted by this invention to solve its technical problem is:

[0007] A device for the co-cultivation of two fungi that induce agarwood formation includes an incubator, characterized in that: a control panel and a delivery pump are respectively provided on one side of the incubator, and a warning light is provided on the other side of the incubator; one side of the incubator is fixedly connected to the surface of the control panel and the surface of the delivery pump respectively, and the other side of the incubator is fixedly connected to the surface of the warning light.

[0008] The incubator is equipped with a production mechanism at the upper end and a quantitative mechanism at the lower end. The production mechanism is used to store the bacterial solution separately, and the quantitative mechanism is used to dispense the solution quantitatively.

[0009] Preferably, the production mechanism includes a bacterial liquid storage tank, a pressing plate, a fixing rod, and a spring. The upper end of the bacterial liquid storage tank is in contact with the lower end of the pressing plate, the inner wall of the pressing plate is slidably connected to the surface of the fixing rod, and the spring is sleeved on the surface of the fixing rod.

[0010] Preferably, the lower end of the bacterial culture tank is fixedly connected to the upper end of the incubator, the lower end of the fixing rod is fixedly connected to the upper end of the incubator, and the two ends of the spring are fixedly connected to the lower end of the pressing plate and the upper end of the incubator, respectively.

[0011] Preferably, the metering mechanism includes a servo motor, a rotating rod, a worm gear, a worm wheel, and a metering plate. The output end of the servo motor is fixedly connected to the surface of the rotating rod, the middle part of the rotating rod is fixedly connected to the surface of the worm gear, the tooth surface of the worm gear meshes with the tooth surface of the worm wheel, and the upper end of the worm wheel is fixedly connected to the lower end of the metering plate.

[0012] Preferably, the surface of the servo motor is fixedly connected to the surface of the incubator, and the surface of the rotating rod is rotatably connected to the inner wall of the incubator.

[0013] Preferably, the surface of the quantitative plate is rotatably connected to the lower end of the bacterial liquid storage tank via a rotating shaft.

[0014] Preferably, a linkage mechanism is provided between the two rotating rods. The linkage mechanism includes an electric push rod, a linkage sleeve, and a sliding rod. The telescopic end of the electric push rod is rotatably connected to the surface of the linkage sleeve through a sliding groove, and the inner wall of the linkage sleeve is slidably connected to the surface of the sliding rod through a sliding groove.

[0015] Preferably, the surface of the electric push rod is fixedly connected to the inner wall of the protective cover inside the incubator, and the surface of the sliding rod is slidably connected to the inner wall of the rotating rod through a sliding groove.

[0016] A method for the synergistic cultivation of two fungi that induce resin formation in agarwood, characterized by the following steps:

[0017] Preset parameters: Set the ratio of the two bacterial cultures, the amount of feed, the timing of feed addition, and the culture period through the control panel;

[0018] Microbial solution storage: The two types of Daphne odora fungus solutions are injected into separate microbial solution storage tanks. The tank openings are sealed by the pressure plate under the action of the spring to prevent cross-contamination and maintain the activity of the microbial solution.

[0019] Quantitative control: The servo motor drives the rotating rod and worm gear to rotate, which in turn drives the quantitative plate to rotate via the worm gear transmission, adjusting the cross section of the bacterial liquid outflow to achieve precise quantitative discharge according to the preset ratio;

[0020] Synchronous feeding: The electric push rod drives the linkage sleeve and sliding rod to move, which drives the two sets of rotating rods to rotate synchronously, so that the two metering plates open and close at the same angle and rhythm, realizing the synchronous feeding of two bacterial solutions;

[0021] Delivery and monitoring: The delivery pump assists in delivering the bacterial solution to the culture area; if the equipment malfunctions, the warning light will immediately sound an alarm to ensure the safety and controllability of the culture process;

[0022] Synergistic cultivation: The two bacterial solutions work synergistically in the culture environment to induce the agarwood tree to activate its defense mechanism and secrete resin, thus achieving efficient and stable resin formation.

[0023] The advantages of this invention are:

[0024] 1. This invention achieves synchronized quantitative feeding of two bacterial solutions through a servo motor, worm gear, and metering plate transmission, combined with a linkage mechanism consisting of an electric push rod, a linkage sleeve, and a sliding rod. The servo motor drives the worm gear to rotate, controlling the rotation angle of the metering plate, thereby adjusting the bacterial solution outflow cross-section and ensuring accurate feeding ratio. The linkage mechanism, through linkage, enables the two feeding devices to operate synchronously, avoiding imbalance caused by asynchronous bacterial solution feeding. This ensures the scientific nature and stability of the bacterial solution ratio during the dual-microbe synergistic culture process, providing an ideal microbial environment for agarwood formation and improving agarwood formation efficiency and quality.

[0025] 2. This invention achieves separate storage of two types of bacterial solutions through independent bacterial solution storage tanks, pressure plates, and spring seals in the production mechanism, effectively preventing cross-contamination and ensuring the activity of the bacterial solutions. At the same time, the coordinated operation of the control panel, delivery pump, and warning lights allows the control panel to set parameters and start / stop the equipment, the delivery pump to assist in the delivery of bacterial solutions, and the warning lights to promptly alarm in abnormal situations. Real-time monitoring improves the safety and controllability of the cultivation process, providing a reliable guarantee for the large-scale and standardized production of agarwood co-cultivation of two bacteria.

[0026] 3. This invention, by setting up a quantitative mechanism consisting of a servo motor, worm gear, and quantitative plate, and cooperating with a mechanical linkage mechanism consisting of an electric push rod, linkage sleeve, and sliding rod, realizes the proportional addition and synchronous control of the dual bacterial solution. This achieves the effect of ensuring scientific ratio and stable feeding of the dual bacterial synergistic culture, and solves the problems of low efficiency and unstable quality of agarwood co-cultivation caused by asynchronous bacterial solution feeding and inaccurate ratio in traditional cultivation equipment. Attached Figure Description

[0027] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0028] Figure 1 This is a schematic diagram of the structure of the present invention;

[0029] Figure 2 This is a schematic diagram of the production mechanism of the present invention;

[0030] Figure 3 This is a schematic diagram of the quantitative mechanism of the present invention;

[0031] Figure 4 This is a schematic diagram of the vertical cross-section of the present invention.

[0032] In the diagram: 1. Incubator; 2. Control panel; 3. Transfer pump; 4. Warning light; 5. Production mechanism; 501. Bacterial solution storage tank; 502. Pressing plate; 503. Fixing rod; 504. Spring; 6. Quantitative mechanism; 601. Servo motor; 602. Rotating rod; 603. Worm gear; 604. Worm wheel; 605. Quantitative plate; 7. Linkage mechanism; 701. Electric push rod; 702. Linkage sleeve; 703. Sliding rod. Detailed Implementation

[0033] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0034] The following is in conjunction with the appendix Figure 1 —4 provides further detailed description of this application.

[0035] This application discloses a device for the co-cultivation of two fungi that induce resin formation in agarwood:

[0036] Reference Figure 1 and Figure 4A dual-fungus co-cultivation device for agarwood resin formation includes an incubator 1. Its innovation lies in the following: a control panel 2 and a delivery pump 3 are respectively installed on one side of the incubator 1, and a warning light 4 is installed on the other side. One side of the incubator 1 is fixedly connected to the surface of the control panel 2 and the surface of the delivery pump 3, respectively, and the other side of the incubator 1 is fixedly connected to the surface of the warning light 4. A production mechanism 5 is installed at the upper end of the incubator 1, and a quantitative mechanism 6 is installed at the lower end of the production mechanism 5. The production mechanism 5 is used for separate storage of the bacterial solution, and the quantitative mechanism 6 is used for quantitative feeding. The bacterial solution is processed by the production mechanism 5. The system features separate storage and quantitative feeding. During feeding, a conveying pump 3 assists in the conveying process. If any abnormality occurs, an alarm light 4 will sound in time. The production mechanism 5 completes the preparation of the bacterial solution. Two different bacterial solutions are injected into separate bacterial solution storage tanks 501 for separate storage. In the working state, the pressing plate 502, under the elastic force of the spring 504 and guided by the fixing rod 503, tightly abuts against the upper opening of the bacterial solution storage tank 501, forming a stable seal. This effectively prevents cross-contamination of the bacterial solution and lays a reliable material foundation for subsequent co-cultivation.

[0037] Reference Figure 2 The production mechanism 5 includes a bacterial solution storage tank 501, a pressing plate 502, a fixing rod 503, and a spring 504. The upper end of the bacterial solution storage tank 501 contacts the lower end of the pressing plate 502. The inner wall of the pressing plate 502 is slidably connected to the surface of the fixing rod 503. The spring 504 is sleeved on the surface of the fixing rod 503. The lower end of the bacterial solution storage tank 501 is fixedly connected to the upper end of the incubator 1. The lower end of the fixing rod 503 is fixedly connected to the upper end of the incubator 1. The two ends of the spring 504 are fixedly connected to the lower end of the pressing plate 502 and the upper end of the incubator 1, respectively. Before starting the equipment, the operator sets the culture parameters through the control panel 2, and then injects two different bacterial solutions into the independent bacterial solution storage tanks 501 in the production mechanism 5 to complete the initial material storage. At this time, the pressing plate 502 is under the elastic force of the spring 504. The pressure plate 502, fixed rod 503, and spring 504 are used to ensure the stable and sealed storage of the bacterial solution in the non-working state, preventing cross-contamination and preparing for subsequent co-cultivation. Through the coordinated arrangement of the bacterial solution storage tank 501, the pressure plate 502, the fixed rod 503, and the spring 504, the independent storage and controllable release of the bacterial solution are achieved. The bacterial solution storage tank 501 is used to store the bacterial solution, the pressure plate 502 is used to seal and open the bacterial solution storage tank 501, the fixed rod 503 is used to guide the sliding of the pressure plate 502, and the spring 504 is used to provide the reset elasticity, ensuring the stability of the bacterial solution in the non-working state, preventing cross-contamination, and realizing the release of the bacterial solution, providing a reliable material guarantee for the co-cultivation of agarwood and resin-forming fungi.

[0038] Reference Figure 3The quantitative mechanism 6 includes a servo motor 601, a rotating rod 602, a worm gear 603, a worm wheel 604, and a quantitative plate 605. The output end of the servo motor 601 is fixedly connected to the surface of the rotating rod 602, the middle part of the rotating rod 602 is fixedly connected to the surface of the worm gear 603, the tooth surface of the worm gear 603 meshes with the tooth surface of the worm wheel 604, the upper end of the worm wheel 604 is fixedly connected to the lower end of the quantitative plate 605, the surface of the servo motor 601 is fixedly connected to the surface of the incubator 1, the surface of the rotating rod 602 is rotatably connected to the inner wall of the incubator 1, and the surface of the quantitative plate 605 is rotatably connected to the lower end of the bacterial culture storage tank 501 via a rotating shaft. The two rotating rods 601... A linkage mechanism 7 is provided between 02 and 02. The linkage mechanism 7 includes an electric push rod 701, a linkage sleeve 702, and a sliding rod 703. The telescopic end of the electric push rod 701 is rotatably connected to the surface of the linkage sleeve 702 through a sliding groove. The inner wall of the linkage sleeve 702 is slidably connected to the surface of the sliding rod 703 through a sliding groove. The surface of the electric push rod 701 is fixedly connected to the inner wall of the protective cover inside the incubator 1. The surface of the sliding rod 703 is slidably connected to the inner wall of the rotating rod 602 through a sliding groove. The control panel 2 starts the servo motor 601. The motor output drives the rotating rod 602 and the worm gear 603 in the middle to rotate. The worm gear 603 and the worm wheel 604 are connected to the rotating rod 602. The meshing transmission transmits power to the worm gear 604, which in turn drives the metering plate 605, which is fixedly connected to it, to rotate. During the rotation, the metering plate 605 controls the outflow cross-section of the bacterial solution through the connection between the rotating shaft and the lower end of the bacterial solution storage tank 501, so as to realize the quantitative addition of the two bacterial solutions according to the preset ratio and ensure the scientific ratio of the dual-bacterial co-culture. In order to ensure that the two bacterial solutions can be added to the incubator 1 synchronously and in a coordinated manner, the linkage mechanism 7 plays a key role. The control panel 2 starts the electric push rod 701, and its extension end drives the linkage sleeve 702 to move through the sliding groove. The inner wall of the linkage sleeve 702 and the surface of the sliding rod 703 are in contact. The surfaces are connected by a sliding groove, which drives the two rotating rods 602 to rotate synchronously, so that the metering plates 605 under the two bacterial liquid storage tanks 501 can move in a consistent manner, ensuring the synchronicity of the two bacterial feeding. Through the coordinated setting of components such as servo motor 601, worm gear 603, worm wheel 604, metering plate 605, electric push rod 701, linkage sleeve 702, and sliding rod 703, this equipment not only realizes the quantitative feeding of bacterial liquid, but also ensures the synchronicity of the two bacterial feeding through the linkage mechanism 7, improving the efficiency and stability of the co-cultivation of agarwood resin-forming bacteria, and providing a reliable guarantee for the cultivation of high-quality agarwood.

[0039] This application also discloses a method for the synergistic cultivation of two fungi that induce agarwood formation:

[0040] An innovative method for the co-cultivation of two fungi in agarwood resin formation involves the following steps: Before starting the equipment, the operator sets the cultivation parameters via the control panel 2. Then, two different fungal solutions are injected into independent storage tanks 501 within the production unit 5, completing the initial material preparation. At this time, the pressing plate 502 remains in its reset state under the elastic force of the spring 504, tightly abutting against the upper opening of the storage tank 501. Combined with the guiding action of the fixing rod 503, this ensures the fungal solution is stably and sealed during non-operational conditions, preventing cross-contamination and preparing for subsequent co-cultivation. The control panel 2 then activates the servo motor 601. The motor output drives the rotating rod 602 and the worm gear 603 in the middle to rotate. Through the meshing transmission between the worm gear 603 and the worm wheel 604, power is transmitted to the worm wheel 604, which in turn drives the fixedly connected worm gear 604. The measuring plate 605 rotates. During this rotation, the measuring plate 605 controls the outflow cross-section of the bacterial solution through the connection between the rotating shaft and the lower end of the bacterial solution storage tank 501, thereby achieving quantitative dispensing of the two bacterial solutions according to a preset ratio and ensuring the scientific nature of the dual-bacterial synergistic culture. To ensure that the two bacterial solutions can be simultaneously and coordinatedly added to the incubator 1, the linkage mechanism 7 plays a key role. The control panel 2 activates the electric push rod 701, whose telescopic end drives the linkage sleeve 702 to move through the sliding groove. The inner wall of the linkage sleeve 702 and the surface of the sliding rod 703 are slidably connected through the sliding groove, thereby driving the two rotating rods 602 to rotate synchronously, so that the measuring plates 605 below the two bacterial solution storage tanks 501 can maintain consistent movement, ensuring the synchronicity of the dual-bacterial feeding. During the feeding process, the delivery pump 3 assists in the delivery. If any abnormality occurs, the warning light 4 will issue an alarm in time.

[0041] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed invention.

Claims

1. A dual-fungus co-cultivation device for agarwood resin formation, comprising an incubator (1), characterized in that: The incubator (1) is equipped with a control panel (2) and a delivery pump (3) on one side, and a warning light (4) on the other side. The incubator (1) is equipped with a production mechanism (5) at the upper end and a quantitative mechanism (6) at the lower end of the production mechanism (5). The production mechanism (5) is used to store the bacterial solution separately, and the quantitative mechanism (6) is used to feed the solution quantitatively.

2. The agarwood resin-forming dual-fungus co-cultivation device according to claim 1, characterized in that: The production mechanism (5) includes a bacterial liquid storage tank (501), a pressing plate (502), a fixing rod (503), and a spring (504). The upper end of the bacterial liquid storage tank (501) is in contact with the lower end of the pressing plate (502). The inner wall of the pressing plate (502) is slidably connected to the surface of the fixing rod (503). The spring (504) is sleeved on the surface of the fixing rod (503).

3. The agarwood resin-forming dual-fungus co-cultivation device according to claim 2, characterized in that: The lower end of the bacterial culture storage tank (501) is fixedly connected to the upper end of the incubator (1), the lower end of the fixing rod (503) is fixedly connected to the upper end of the incubator (1), and the two ends of the spring (504) are fixedly connected to the lower end of the pressing plate (502) and the upper end of the incubator (1) respectively.

4. The agarwood resin-forming dual-fungus co-cultivation device according to claim 2, characterized in that: The metering mechanism (6) includes a servo motor (601), a rotating rod (602), a worm (603), a worm wheel (604), and a metering plate (605). The output end of the servo motor (601) is fixedly connected to the surface of the rotating rod (602). The middle part of the rotating rod (602) is fixedly connected to the surface of the worm (603). The tooth surface of the worm (603) meshes with the tooth surface of the worm wheel (604). The upper end of the worm wheel (604) is fixedly connected to the lower end of the metering plate (605).

5. The agarwood resin-forming dual-fungus co-cultivation device according to claim 4, characterized in that: The surface of the servo motor (601) is fixedly connected to the surface of the incubator (1), and the surface of the rotating rod (602) is rotatably connected to the inner wall of the incubator (1).

6. The agarwood resin-forming dual-fungus co-cultivation device according to claim 5, characterized in that: The surface of the quantitative plate (605) is rotatably connected to the lower end of the bacterial liquid storage tank (501) via a rotating shaft.

7. The agarwood resin-forming dual-fungus co-cultivation device according to claim 4, characterized in that: A linkage mechanism (7) is provided between the two rotating rods (602). The linkage mechanism (7) includes an electric push rod (701), a linkage sleeve (702), and a sliding rod (703). The telescopic end of the electric push rod (701) is rotatably connected to the surface of the linkage sleeve (702) through a sliding groove, and the inner wall of the linkage sleeve (702) is slidably connected to the surface of the sliding rod (703) through a sliding groove.

8. The agarwood resin-forming dual-fungus co-cultivation device according to claim 7, characterized in that: The surface of the electric push rod (701) is fixedly connected to the inner wall of the protective cover inside the incubator (1), and the surface of the sliding rod (703) is slidably connected to the inner wall of the rotating rod (602) through a sliding groove.

9. A method for the synergistic cultivation of two fungi that induce agarwood formation, characterized in that: This method is based on the co-cultivation method of two fungi for agarwood formation as described in any one of claims 1-8, and includes the following steps: Parameter preset: Set the ratio of the two bacterial solutions, the amount of feed, the timing of feed and the culture cycle through the control panel (2); Fungal solution storage: The two types of Aromatic Agaricus dahurica fungus solutions are injected into separate fungal solution storage tanks (501). The pressure plate (502) seals the tank opening under the action of the spring to prevent cross-contamination and maintain the activity of the fungal solution. Quantitative control: The servo motor (601) drives the rotating rod (602) and the worm (603) to rotate, and the worm wheel (604) drives the quantitative plate (605) to rotate, adjusting the cross section of the bacterial liquid outflow, so as to achieve precise quantitative discharge according to the preset ratio; Synchronous feeding: The electric push rod (701) drives the linkage sleeve (702) and the sliding rod (703) to move, which drives the two sets of rotating rods (602) to rotate synchronously, so that the two metering plates (605) open and close at the same angle and in the same rhythm, realizing the synchronous feeding of the two bacterial solutions; Delivery and monitoring: The delivery pump (3) assists in delivering the bacterial solution to the culture area; when the equipment malfunctions, the warning light (4) will immediately sound an alarm to ensure the safety and controllability of the culture process; Synergistic cultivation: The two bacterial solutions work synergistically in the culture environment to induce the agarwood tree to activate its defense mechanism and secrete resin, thus achieving efficient and stable resin formation.

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