A nucleic acid detection sample pretreatment integrated device for screening of ducklings

Abstract

The application discloses a nucleic acid detection sample pretreatment integrated device for screening of breeding ducks, and belongs to the technical field of nucleic acid detection. The device comprises a casing, a clamping pipe assembly is arranged on the inner side of the casing, a sampling assembly and a cup separating assembly are arranged on the inner side of the upper end of the casing through sliding on a track, an extraction assembly and a deep hole plate are further arranged on the inner side of the casing, the extraction assembly comprises an adjusting mechanism, a magnetic bar and a fixed plate frame are respectively fixedly arranged on the adjusting mechanism, guide sleeves are uniformly and fixedly arranged on the fixed plate frame, a magnetic bar sleeve is rotatably connected to the lower end of the guide sleeve, and a sealing assembly for sealing the pipe opening of the deep hole plate is connected to the lower side of the fixed plate frame through an elastic element. The nucleic acid detection sample pretreatment integrated device for screening of breeding ducks can automatically seal the pipe opening during the extraction process, and the motion mode of the magnetic bar sleeve is optimized, so that the cross contamination risk is reduced and the extraction effect is effectively improved.

Description

Technical Field

[0001] This invention relates to the field of nucleic acid detection technology, specifically to an integrated device for nucleic acid detection sample preprocessing used in the screening of breeding ducks. Background Technology

[0002] Breeding and disease screening of ducks are core components for improving the quality and efficiency of modern large-scale poultry farming. Relying on nucleic acid detection technology for identifying the purity of breeding duck breeds and screening for viral diseases, it has become an indispensable core testing method for breeding duck qualification review and population optimization screening due to its advantages of high sensitivity, strong specificity, and accurate result interpretation. Nucleic acid sample pretreatment, as a crucial preliminary step in the nucleic acid detection process, directly determines the accuracy and stability of subsequent PCR amplification results through sample lysis, nucleic acid extraction purity, and extraction efficiency. Magnetic rod-and-bead nucleic acid extraction structures, with their strong adaptability and good purification effect, have been widely used in nucleic acid sample pretreatment equipment in various livestock and poultry farming fields.

[0003] Existing magnetic bead extraction mechanisms used in pretreatment equipment for nucleic acid testing samples in breeding ducks generally employ a vertically reciprocating lifting structure with a magnetic rod and a magnetic sleeve. The entire extraction process—including magnetic bead adsorption, transfer, washing, and nucleic acid elution—is completed by switching the magnetic field on and off with the magnetic rod in conjunction with the up-and-down movement of the magnetic sleeve. To avoid liquid leakage during extraction, traditional equipment typically only has a fixed drip protection plate below the magnetic sleeve to catch any dripping waste liquid, providing simple waste liquid interception and protection, which can meet the basic extraction needs of routine small-batch nucleic acid samples.

[0004] The magnetic sleeve only performs a single vertical lifting and lowering movement, and the sample tube opening is in an open state throughout the operation. During the rapid insertion and removal of the magnetic sleeve from the test tube, the sample solution inside the test tube is easily agitated, causing liquid to splash upwards and overflow. In an open environment, the splashed liquid can easily form aerosols, which not only cause sample loss and reagent waste, but also spread throughout the equipment, causing cross-contamination between different groups of samples, seriously affecting the authenticity of the nucleic acid test results of the breeding ducks. Secondly, the traditional fixed drip protection plate can only passively catch the dripping liquid and cannot prevent the liquid from leaking out at the source. The residual liquid attached to the outer wall of the magnetic sleeve can easily drip onto the internal table of the equipment during the upward process. The dripping liquid will still quickly form diffuse aerosols, continuously aggravating the overall contamination level inside the equipment and significantly increasing the sample detection error rate. Summary of the Invention

[0005] The purpose of this invention is to provide an integrated nucleic acid detection sample pretreatment device for screening breeding ducks, in order to solve the problems mentioned in the background art, where the magnetic sleeve only performs a single vertical lifting and lowering movement, the sample tube opening is in an open state throughout the operation, and the sample solution inside the test tube is easily agitated during the rapid insertion and removal of the magnetic sleeve, causing liquid to splash upwards and overflow. In an open environment, the splashed liquid is very likely to form aerosols, which not only causes sample loss and reagent waste, but also spreads throughout the equipment, causing cross-contamination between different groups of samples, seriously affecting the authenticity of the nucleic acid detection results of breeding ducks.

[0006] To achieve the above objectives, the present invention provides the following technical solution: an integrated device for nucleic acid detection sample pretreatment for duck selection, comprising a housing, a tube clamping assembly installed inside the housing, and a sampling assembly and a cup-dispensing assembly slidably arranged on the upper inner side of the housing via a track; an extraction assembly for extracting nucleic acid and a deep-well plate are also provided inside the housing; the extraction assembly includes an adjustment mechanism, on which a magnetic rod and a fixed plate are respectively fixedly installed, and the adjustment mechanism controls the magnetic rod and the fixed plate to perform independent lifting and lowering adjustment; a guide sleeve is uniformly and fixedly arranged through the fixed plate, and a magnetic rod sleeve is rotatably connected to the lower end of the guide sleeve; a sealing assembly for sealing the deep-well plate tube opening is connected to the lower part of the fixed plate via an elastic element, and the sealing assembly is sleeved on the outside of the magnetic rod sleeve; a transmission assembly is provided between the magnetic rod sleeve and the sealing assembly, and the magnetic rod sleeve is driven to rotate by the transmission assembly when the magnetic rod sleeve and the sealing assembly slide relative to each other.

[0007] Preferably, the adjustment mechanism includes a movable seat slidably disposed inside the housing, and a first lifting frame and a second lifting frame are respectively mounted on the movable seat. A magnetic rod is fixedly mounted on the first lifting frame, and a fixed plate frame is fixedly mounted on the second lifting frame.

[0008] Preferably, the diameter of the guide sleeve gradually decreases from top to bottom, and the inner diameter of the guide sleeve is larger than the outer diameter of the magnetic rod, and the magnetic rod, guide sleeve and magnetic rod sleeve are coaxially arranged.

[0009] Preferably, the sealing assembly includes a movable plate, and a sealing plug is uniformly fixedly installed on the lower surface of the movable plate. The shape of the sealing plug matches the opening of the deep hole plate, and the sealing plug is inserted into the opening of the deep hole plate during the downward movement of the sealing plug.

[0010] Preferably, the magnetic rod sleeve extends through the movable plate and the sealing plug, and the magnetic rod sleeve and the sealing plug are slidably and sealingly connected.

[0011] Preferably, the transmission assembly includes a transmission kit fixedly sleeved on the outer side of the upper part of the magnetic rod sleeve, and a spiral guide groove is provided on the outer side of the transmission kit. A ball bearing is embedded in the inner wall of the through hole on the movable plate, and the ball bearing is slidably disposed on the inner side of the guide groove. During the lifting and lowering adjustment of the transmission kit driven by the magnetic rod sleeve, the ball bearing rolls along the guide groove, and the ball bearing drives the magnetic rod sleeve to rotate synchronously during the rolling along the guide groove.

[0012] Preferably, a gas supply pipe is provided through the movable plate and the sealing plug, and the lower end of the gas supply pipe is connected to the inside of the tube on the deep hole plate. A one-way valve is provided on the gas supply pipe, and during the process of the magnetic rod moving down inside the tube of the deep hole plate, some gas is squeezed out through the gas supply pipe.

[0013] Preferably, the lower surface of the fixed plate frame is provided with a gas collecting seat, and the inner side of the gas collecting seat is provided with a cavity. The cavity inside the gas collecting seat is connected to the gas supply pipe, and the cavity inside the gas collecting seat is also connected to an external filtration and purification mechanism through a pipe.

[0014] Preferably, the magnetic rod sleeve penetrates the sealing plug and slides in a sealing fit with the sealing plug. The one-way valve provided on the gas supply pipe is a one-way valve that allows unidirectional flow from the cavity of the deep hole plate to the gas collecting seat. The cavity of the deep hole plate, the gas supply pipe, the one-way valve, and the gas collecting seat together form a one-way exhaust structure.

[0015] Compared with the prior art, the beneficial effects of the present invention are: the integrated nucleic acid detection sample pretreatment device for duck selection can automatically seal the tube opening during the extraction process, and by optimizing the movement mode of the magnetic rod sleeve, it reduces the risk of cross-contamination while effectively improving the extraction effect. The specific details are as follows; 1. Equipped with a movable plate and a sealing plug, by controlling the fixed plate frame and the magnetic rod sleeve to move down synchronously, the sealing plug on the movable plate can be inserted into the corresponding position of the tube opening on the deep hole plate, thereby effectively avoiding sample liquid splashing and aerosol overflow contamination caused by the subsequent movement of the magnetic rod sleeve, and ensuring the accuracy of subsequent detection.

[0016] 2. Equipped with a magnetic rod sleeve, a transmission assembly, and a movable plate, when the sealing plug completes the sealing of the tube opening, the fixed plate frame drives the magnetic rod sleeve to move down synchronously. This allows the movable plate to slide and adjust on the outside of the magnetic rod sleeve and the transmission assembly. At this time, the balls on the movable plate will roll along the spiral guide groove of the transmission assembly, which in turn drives the magnetic rod sleeve to rotate. This allows the magnetic rod sleeve to rotate while reciprocating up and down. The up and down movement brings axial liquid flow impact, and the rotation forms a circumferential vortex. The superposition of the two phases of motion breaks up the magnetic beads and ensures more thorough contact between the reagent, sample, and magnetic beads, avoiding uneven local concentration and effectively improving the nucleic acid extraction effect.

[0017] Furthermore, when the magnetic rod sleeve is removed, it rotates synchronously as it rises above the liquid surface, which helps to shake off excess solution from the outside of the sleeve, preventing solution dripping during operation and reducing the risk of contamination inside the equipment.

[0018] 3. It is equipped with a gas collecting seat and a gas delivery pipe. As the magnetic rod sleeve moves down inside the tube of the deep well plate, it will squeeze the excess gas into the gas collecting seat through the gas delivery pipe. The subsequent reciprocating rise and fall of the magnetic rod sleeve can cause the gas pressure inside the tube of the deep well plate to change repeatedly, which can further promote cell lysis and substance precipitation and improve extraction efficiency.

[0019] Furthermore, when the magnetic rod sleeve is removed, the inside of the deep hole plate is under negative pressure, which prevents residual aerosol from overflowing when the sealing plug is released, thus enhancing the anti-pollution effect. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the main structure of the present invention; Figure 2 This is a schematic diagram of the installation structure of the sampling component, the cup-dividing component, and the extraction component of the present invention; Figure 3 This is a schematic diagram of the extracted component structure of the present invention; Figure 4 This is a schematic diagram of the mounting structure of the fixed plate frame and deep hole plate of the present invention; Figure 5 This is a schematic diagram of the connection structure between the fixing plate frame and the magnetic rod sleeve of the present invention; Figure 6 This is a schematic diagram of the connection structure between the movable plate and the sealing plug of the present invention; Figure 7 This is a schematic cross-sectional view of the magnetic rod sleeve and deep hole plate of the present invention; Figure 8 This is a schematic diagram of the connection structure between the magnetic rod sleeve and the transmission assembly of the present invention; Figure 9 This is a schematic diagram of the transmission kit and ball connection structure of the present invention.

[0021] In the diagram: 1. Housing; 2. Sampling assembly; 3. Clamping tube assembly; 4. Cup dispensing assembly; 5. Extraction assembly; 6. Moving seat; 7. First lifting frame; 8. Magnetic rod; 9. Second lifting frame; 10. Fixed plate frame; 1001. Gas collecting seat; 11. Guide sleeve; 12. Magnetic rod sleeve; 13. Transmission kit; 1301. Guide groove; 14. Movable plate; 1401. Ball bearing; 15. Sealing plug; 16. Deep hole plate; 17. Gas delivery pipe. Detailed Implementation

[0022] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0023] Please see Figures 1-2 As shown, the present invention discloses the following technical content: an integrated device for nucleic acid detection sample pretreatment for duck selection, including a housing 1, a clamping tube assembly 3 installed inside the housing 1, and a sampling assembly 2 and a cupping assembly 4 slidably arranged on the upper inner side of the housing 1 via a track. The inner side of the housing 1 is also provided with an extraction assembly 5 for extracting nucleic acid and a deep well plate 16.

[0024] The sampling component 2 can slide along the track on the top of the housing 1 and use its mechanical claw to grab and transfer the sample tube. The tube clamping component 3 can clamp and fix the lower end of the tube. At the same time, in conjunction with the mechanical claw on the sampling component 2, it can automatically open the tube. The dispensing component 4 can also slide along the track and use a pipette to dispense the sample in the tube. The extraction component 5 can extract and clean the sample inside the deep well plate 16.

[0025] like Figures 2-3 As shown, the extraction component 5 includes an adjustment mechanism, on which a magnetic rod 8 and a fixed plate frame 10 are fixedly mounted respectively. The adjustment mechanism controls the magnetic rod 8 and the fixed plate frame 10 to perform independent lifting and lowering adjustments. A guide sleeve 11 is uniformly and fixedly disposed through the fixed plate frame 10. The lower end of the guide sleeve 11 is rotatably connected to a magnetic rod sleeve 12. The adjustment mechanism includes a movable seat 6 that is slidably disposed inside the housing 1. A first lifting frame 7 and a second lifting frame 9 are respectively lifted and lowered on the movable seat 6. The magnetic rod 8 is fixedly mounted on the first lifting frame 7, and the fixed plate frame 10 is fixedly mounted on the second lifting frame 9. The diameter of the guide sleeve 11 gradually decreases from top to bottom, and the inner diameter of the guide sleeve 11 is larger than the outer diameter of the magnetic rod 8. The magnetic rod 8, the guide sleeve 11, and the magnetic rod sleeve 12 are coaxially arranged.

[0026] During extraction, the horizontal positions of the first lifting frame 7 and the second lifting frame 9 can be adjusted by controlling the movable seat 6 to ensure that the magnetic rod 8 and the magnetic rod sleeve 12 can correspond to the deep hole plate 16 at different positions. By controlling the lifting and lowering of the first lifting frame 7 and the second lifting frame 9 on the movable seat 6, the vertical positions of the magnetic rod 8 and the magnetic rod sleeve 12 can be controlled independently, so that the magnetic rod sleeve 12 can be vertically inserted into the tube of the deep hole plate 16 to achieve stirring and extraction. During the downward movement of the magnetic rod 8, it can be inserted into the interior of the magnetic rod sleeve 12, thereby achieving the adsorption of magnetic beads.

[0027] like Figures 3-6 As shown, a sealing assembly for sealing the opening of the deep hole plate 16 is connected to the lower part of the fixed plate frame 10 via an elastic element. The sealing assembly is sleeved on the outside of the magnetic rod sleeve 12. The sealing assembly includes a movable plate 14, and a sealing plug 15 is uniformly fixedly installed on the lower surface of the movable plate 14. The shape of the sealing plug 15 matches the opening of the deep hole plate 16. During the downward movement of the sealing plug 15, it is sealed and inserted into the opening of the deep hole plate 16.

[0028] As the second lifting frame 9 moves downward, the fixed plate frame 10 can drive the magnetic rod sleeve 12 to move downward through the guide sleeve 11. At the same time, the fixed plate frame 10 can drive the movable plate 14 to move downward synchronously through the elastic element, so that the sealing plug 15 on the lower surface of the movable plate 14 can be sealed and inserted into the corresponding pipe opening on the deep hole plate 16, thereby avoiding liquid splashing and aerosol overflow pollution during subsequent stirring.

[0029] like Figures 4-5 and Figures 8-9 As shown, a transmission assembly is provided between the magnetic rod sleeve 12 and the sealing assembly. When the magnetic rod sleeve 12 and the sealing assembly slide relative to each other for adjustment, the magnetic rod sleeve 12 is driven to rotate through the transmission assembly. The magnetic rod sleeve 12 rises and falls through the movable plate 14 and the sealing plug 15, and the magnetic rod sleeve 12 and the sealing plug 15 are slidably sealed together. The transmission assembly includes a transmission kit 13 fixedly sleeved on the upper outer side of the magnetic rod sleeve 12, and a spiral guide groove 1301 is provided on the outer side of the transmission kit 13. A ball bearing 1401 is embedded in the inner wall of the through hole on the movable plate 14, and the ball bearing 1401 is slidably disposed on the inner side of the guide groove 1301. During the process of the magnetic rod sleeve 12 driving the transmission kit 13 to rise and fall for adjustment, the ball bearing 1401 rolls along the guide groove 1301, and the ball bearing 1401 drives the magnetic rod sleeve 12 to rotate synchronously during the rolling along the guide groove 1301.

[0030] After the sealing plug 15 seals the opening of the deep hole plate 16, the fixed plate frame 10 continues to move downward. At this time, the magnetic rod sleeve 12 and the transmission assembly 13 will move relative to the movable plate 14 and the sealing plug 15. The ball bearings 1401 provided on the inner wall of the through hole on the movable plate 14 can roll along the guide groove 1301 opened on the outside of the transmission assembly 13. Since the guide groove 1301 is spirally arranged, the ball bearings 1401 can drive the transmission assembly 13 to drive the magnetic rod sleeve 12 to rotate synchronously during the rolling process. Then, the lower part of the magnetic rod sleeve 12 will be immersed in the solution inside the tube of the deep hole plate 16. Then, the second lifting frame 9 is controlled to drive the magnetic rod sleeve 12 to reciprocate up and down. At this time, the magnetic rod sleeve 12 will also rotate synchronously. The reciprocating up and down and rotation of the magnetic rod sleeve 12 can improve the stirring effect. The lifting brings axial liquid flow impact, and the rotation forms a circumferential vortex. The two phases of motion overlap. The process involves breaking up the aggregated magnetic beads to ensure better contact between the reagents, samples, and magnetic beads, preventing uneven local concentrations. Then, the first lifting frame 7 moves the magnetic rod 8 downwards, inserting it into the inside of the magnetic rod sleeve 12. This allows the nucleic acid-bound magnetic beads to adhere to the bottom of the sleeve 12. The first and second lifting frames 7 and 9 then simultaneously lift the magnetic rod 8 and sleeve 12, transferring them horizontally above the next deep-well plate 16. The magnetic rod 8 and sleeve 12 then move downwards together into the washing solution. The magnetic rod 8 is then withdrawn, and the sleeve 12 is moved up and down again to ensure sufficient contact between the magnetic beads and the washing solution for impurity elution. The magnetic rod 8 then moves downwards again into the sleeve 12, adsorbing the magnetic beads again, and repeating the "collect-transfer-release" process into the elution wells. Finally, the magnetic rod adsorbs and removes the magnetic beads, retaining the elution solution containing purified nucleic acid.

[0031] like Figures 7-9 A gas supply pipe 17 is provided through the movable plate 14 and the sealing plug 15, and the lower end of the gas supply pipe 17 is connected to the inside of the tube body of the deep hole plate 16. A one-way valve is provided on the gas supply pipe 17. During the process of the magnetic rod sleeve 12 moving down inside the tube body of the deep hole plate 16, some gas is squeezed out through the gas supply pipe 17. The magnetic rod sleeve 12 passes through the sealing plug 15 and slides and seals with the sealing plug 15. The one-way valve provided on the gas supply pipe 17 is a one-way valve that conducts unidirectionally from the cavity of the deep hole plate 16 to the gas collecting seat 1001. The cavity of the deep hole plate 16, the gas supply pipe 17, the one-way valve and the gas collecting seat 1001 together form a one-way exhaust structure.

[0032] During the lysis and binding process, as the magnetic rod sleeve 12 moves downward, excess gas in the deep well plate 16 can be squeezed out through the gas delivery pipe 17. Since a one-way valve is provided on the gas delivery pipe 17, when the magnetic rod sleeve 12 moves upward, the gas pressure in the deep well plate 16 decreases. As the magnetic rod sleeve 12 moves up and down to stir, the gas pressure in the deep well plate 16 changes back and forth, which helps to promote cell lysis and nucleic acid precipitation. When the magnetic rod sleeve 12 is removed from the deep well plate 16, the deep well plate 16 is under negative pressure. When the sealing plug 15 is removed from the tube opening, the negative pressure in the tube can further reduce the overflow of aerosols.

[0033] like Figure 5 As shown, a gas collecting seat 1001 is provided on the lower surface of the fixed plate frame 10, and a cavity is provided on the inner side of the gas collecting seat 1001. The cavity inside the gas collecting seat 1001 is connected to the gas supply pipe 17, and the cavity inside the gas collecting seat 1001 is also connected to an external filter purification mechanism through a pipe.

[0034] The gas collection seat 1001 can collect the aerosol-containing gas discharged from each gas pipe 17 and transport it through the pipeline to an external filtration and purification unit, thereby filtering and killing pollutants, achieving purified discharge, and avoiding pollution.

[0035] The contents not described in detail in this specification are existing technologies known to those skilled in the art.

[0036] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. An integrated device for nucleic acid detection sample pretreatment for duck selection, comprising a housing (1), wherein a clamping tube assembly (3) is installed on the inner side of the housing (1), and a sampling assembly (2) and a cup-separating assembly (4) are slidably arranged on the inner side of the upper end of the housing (1) via a track, and an extraction assembly (5) and a deep well plate (16) for extracting nucleic acid are also provided on the inner side of the housing (1). Its features are, The extraction component (5) includes an adjustment mechanism, on which a magnetic rod (8) and a fixed plate frame (10) are fixedly installed respectively. The adjustment mechanism controls the magnetic rod (8) and the fixed plate frame (10) to perform independent lifting and lowering adjustments. A guide sleeve (11) is uniformly and fixedly installed through the fixed plate frame (10). A magnetic rod sleeve (12) is rotatably connected to the lower end of the guide sleeve (11). A sealing component for sealing the opening of the deep hole plate (16) is connected to the lower part of the fixed plate frame (10) through an elastic element. The sealing component is sleeved on the outside of the magnetic rod sleeve (12). A transmission component is provided between the magnetic rod sleeve (12) and the sealing component. When the magnetic rod sleeve (12) and the sealing component slide relative to each other, the magnetic rod sleeve (12) is driven to rotate through the transmission component.

2. The integrated nucleic acid detection sample preprocessing device for duck selection according to claim 1, characterized in that: The adjustment mechanism includes a movable seat (6) that is slidably disposed inside the housing (1), and a first lifting frame (7) and a second lifting frame (9) are respectively mounted on the movable seat (6). A magnetic rod (8) is fixedly mounted on the first lifting frame (7), and a fixed plate frame (10) is fixedly mounted on the second lifting frame (9).

3. The integrated device for nucleic acid detection sample preprocessing for duck selection according to claim 1, characterized in that: The diameter of the guide sleeve (11) gradually decreases from top to bottom, and the inner diameter of the guide sleeve (11) is larger than the outer diameter of the magnetic rod (8). The magnetic rod (8), the guide sleeve (11) and the magnetic rod sleeve (12) are coaxially arranged.

4. The integrated device for nucleic acid detection sample preprocessing for duck selection according to claim 1, characterized in that: The sealing assembly includes a movable plate (14), and a sealing plug (15) is uniformly fixedly installed on the lower surface of the movable plate (14). The shape of the sealing plug (15) matches the opening of the deep hole plate (16), and the sealing plug (15) is inserted into the opening of the deep hole plate (16) during the downward movement.

5. The integrated device for nucleic acid detection sample preprocessing for duck selection according to claim 4, characterized in that: The magnetic rod sleeve (12) moves up and down through the movable plate (14) and the sealing plug (15), and the magnetic rod sleeve (12) and the sealing plug (15) are slidably sealed together.

6. The integrated device for nucleic acid detection sample preprocessing for duck selection according to claim 5, characterized in that: The transmission assembly includes a transmission kit (13) fixedly sleeved on the outer side of the upper part of the magnetic rod sleeve (12), and a spiral guide groove (1301) is provided on the outer side of the transmission kit (13). A ball bearing (1401) is embedded in the inner wall of the through hole on the movable plate (14), and the ball bearing (1401) is slidably disposed on the inner side of the guide groove (1301). During the lifting and lowering adjustment process of the magnetic rod sleeve (12) driving the transmission kit (13), the ball bearing (1401) rolls along the guide groove (1301), and the ball bearing (1401) drives the magnetic rod sleeve (12) to rotate synchronously during the rolling process along the guide groove (1301).

7. The integrated nucleic acid detection sample preprocessing device for duck selection according to claim 4, characterized in that: A gas delivery pipe (17) is provided through the movable plate (14) and the sealing plug (15), and the lower end of the gas delivery pipe (17) is connected to the inside of the tube body of the deep hole plate (16). A one-way valve is provided on the gas delivery pipe (17). During the process of the magnetic rod sleeve (12) moving down inside the tube body of the deep hole plate (16), some gas is squeezed out through the gas delivery pipe (17).

8. The integrated device for nucleic acid detection sample preprocessing for duck selection according to claim 7, characterized in that: The lower surface of the fixed plate frame (10) is provided with a gas collecting seat (1001), and the inner side of the gas collecting seat (1001) is provided with a cavity. The cavity inside the gas collecting seat (1001) is connected to the gas supply pipe (17). The cavity inside the gas collecting seat (1001) is also connected to an external filtration and purification mechanism through a pipe.

9. The integrated device for nucleic acid detection sample preprocessing for duck selection according to claim 8, characterized in that: The magnetic rod sleeve (12) penetrates the sealing plug (15) and slides and seals with the sealing plug (15). The one-way valve on the gas pipe (17) is a one-way valve that conducts unidirectionally from the cavity of the deep hole plate (16) to the gas collecting seat (1001). The cavity of the deep hole plate (16), the gas pipe (17), the one-way valve and the gas collecting seat (1001) together form a one-way exhaust structure.

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