A batch culture device for microbial detection

Abstract

The present application relates to the technical fields of microbial culture, and specifically relates to a batch culture device for microbial detection, which comprises a main box body, a base fixedly installed at the bottom of the inner cavity of the main box body, an installation cylinder rotationally connected to the top of the base, a rotating disc fixedly installed on the outer circumferential surface of the installation cylinder, a plurality of horizontal rods arranged at equal angles on the outer circumferential surface of the rotating disc, a connecting rod rotationally connected to each horizontal rod, and a cooling box welded to the side of each connecting rod away from the rotating disc; in use, a researcher only needs to complete the inoculation of a single test tube through the glove operation windows on both sides of the box body, and place the test tube in the corresponding station of the rotary structure; the rotary structure will drive the test tube to continuously flow along the preset path, and automatically complete all the rolling tube sample preparation processes such as overturning, shaking, mixing, cooling and continuous rolling in sequence during the travel process, without manual intervention in the subsequent processing flow.

Description

Technical Field

[0001] This invention relates to the field of microbial culture technology, specifically to a batch culture device for microbial detection. Background Technology

[0002] Anaerobic microbial culture systems are core experimental equipment for conducting research and industrial applications of strictly anaerobic bacteria. Relying on the synergistic effect of a closed operating culture chamber, gas replacement system, and catalytic deoxygenation unit, a stable, oxygen-free, constant-temperature, and highly clean growth environment can be continuously constructed within the chamber. The entire process, including strain inoculation, isolation and purification, and culture observation, can be completed without disrupting the anaerobic atmosphere. It is an indispensable basic device in fields such as environmental microbiology analysis, clinical anaerobic pathogen detection, and industrial functional bacteria screening, directly determining the feasibility and reliability of anaerobic bacteria culture experiments. In the isolation, purification, and batch culture of anaerobic bacteria, the Hungate rolling tube technique has become one of the mainstream technical solutions for strictly anaerobic bacteria culture due to its excellent oxygen isolation effect and good single-colony isolation effect. However, under the current operating mode, the core steps of rolling tube sampling largely rely on manual labor by researchers: each tube must be individually... The target bacterial strain is inoculated into an anaerobic test tube containing molten culture medium. The bacterial solution is thoroughly mixed with the culture medium by manually inverting and shaking the tube. The tube is then transferred to a cooling process and kept rolling until the agar completely solidifies, forming a uniform thin culture layer on the tube wall. Due to the physical properties of agar, the rolling sample collection has strict temperature window requirements. Molten agar gradually loses its fluidity and solidifies as the ambient temperature decreases. The manual processing of each tube not only prolongs the overall preparation cycle of a single batch of test tubes but also easily leads to multi-dimensional deviations in culture consistency. Differences in inoculation time, mixing intensity and amplitude, and timing of entering the cooling process among different test tubes directly result in inconsistent agar layer thickness and bacterial spatial distribution among the tubes. Therefore, this invention provides a batch culture device for microbial detection. Summary of the Invention

[0003] The purpose of this invention is to provide a batch culture device for microbial detection to solve the problems mentioned in the background art.

[0004] The technical solution of this invention is: a batch culture device for microbial detection, comprising a main chamber, a base fixedly installed at the bottom of the inner cavity of the main chamber, an installation cylinder rotatably connected to the top of the base, a turntable fixedly installed on the outer circular surface of the installation cylinder, and connecting rods rotatably connected to several crossbars arranged at equal angles on the outer circular surface of the turntable. A cooling box is welded to the side of each connecting rod away from the turntable, a waterproof plastic film is provided on the inner circular surface of each cooling box, and driven gears are rotatably connected to the upper and lower ends of each cooling box. Several elastic rubber blocks are arranged at equal angles on the inner circular surface of each driven gear. When using this invention, researchers only need to complete the inoculation of a single test tube with bacteria through the glove operation window on both sides of the chamber and place it in the corresponding position of the rotating structure. The rotating structure will then drive the test tube to continuously flow along a preset path, automatically completing all the rolling sample-taking processes such as inverting, shaking and mixing, and continuous rolling during the cooling stage in sequence during the movement, without the need for manual intervention in the subsequent processing.

[0005] Preferably, a drive motor is fixedly installed on the top of the base, and a drive gear is fixedly installed on the top output end of the drive motor. A drive gear ring that meshes with the drive gear is fixedly sleeved on the outer circular surface of the mounting cylinder. The drive motor outputs power to drive the drive gear to rotate at low speed. Through gear meshing transmission, the drive gear ring, mounting cylinder, and turntable rotate synchronously, and then the cooling box and test tube are driven to revolve along the circumferential path via the connecting rod.

[0006] Preferably, a plurality of support rods are fixedly installed at equal angles on the top of the base, and a curved ring is fixedly installed between the tops of the plurality of support rods. A plurality of mounting brackets are welded at equal angles on the bottom of the turntable. A sliding rod is slidably arranged on the side wall of each mounting bracket. A movable rack is welded to the side of each sliding rod near the curved ring. A spring is wound around the body of each sliding rod, and a plurality of springs are respectively connected between the corresponding movable rack and the mounting bracket. A flipping gear ring that meshes with the corresponding movable rack is welded to each connecting rod. A curved ring with multiple protrusions is arranged along the revolution path of the test tube. When the test tube revolves with the turntable to the protrusion position of the curved ring, the movable rack slides outward along the protrusion trajectory, driving the flipping gear ring and the connecting rod that mesh with it to rotate, thereby driving the cooling box and the test tube to complete a 90-degree rotation.

[0007] Preferably, a column is welded to the center of the top of the base, and a fixed gear ring is welded to the outer surface of the column through several crossbars arranged at equal angles. Several vertical shafts are rotatably connected to the top of the turntable at equal angles. Each vertical shaft is fixedly fitted with a linkage gear that meshes with the fixed gear ring. A fixed friction disc is fixedly installed on the top of each vertical shaft. The vertical shafts and linkage gears on the turntable revolve synchronously with the turntable. Since the linkage gear meshes with the fixed gear ring, the linkage gear rotates simultaneously during the revolution.

[0008] Preferably, a C-shaped frame is welded to the back of each cooling box, a sleeve is rotatably connected to the side wall of each C-shaped frame, a sleeve rod is slidably disposed inside each sleeve, a movable friction disc is fixedly installed on the side of each sleeve rod away from the corresponding sleeve, a second spring is wound around the body of each sleeve rod, and several second springs are respectively located between the corresponding movable friction disc and the corresponding sleeve, and a bevel gear is fixedly installed on the side of each sleeve away from the corresponding sleeve rod.

[0009] Preferably, each of the connecting rods is rotatably connected to a vertical rod, and each vertical rod is fixedly equipped with a bevel gear two that meshes with bevel gear one. Each vertical rod has two symmetrical driving gears fixedly installed at its upper and lower ends, and several driving gears mesh with corresponding driven gears. After the test tube is flipped to a horizontal state, the movable friction disk abuts against the fixed friction disk. The rotational power of the fixed friction disk is transmitted to the sleeve rod and sleeve through the movable friction disk, and then the vertical rod and driving gear rotate synchronously through the reversing transmission of bevel gear one and bevel gear two. The driving gear meshes and drives the driven gear to rotate, and finally the test tube completes a short-term self-rotation through the elastic rubber block. When the test tube passes through multiple sets of protruding structures, it can cycle through the inverting and shaking and short-term self-rotation actions, realizing the combined effect of inverting and mixing and rotating and homogenizing, so that the bacterial solution and the molten culture medium are mixed more evenly and thoroughly.

[0010] Preferably, a distribution box is rotatably connected to the top of the column, and a top gear is fixedly installed on the top of the distribution box. Water supply hoses connect the distribution box to several cooling boxes. A manifold is rotatably connected to the top of the distribution box. When the test tube revolves to the continuous protruding area of ​​the curved ring, the test tube maintains a 90-degree rotation and continues to rotate. Simultaneously, the electrically controlled valve corresponding to the water supply hose in the distribution box opens, and cooling water flows into the cooling box through the water supply hose. It then fully contacts the outer wall of the test tube through the waterproof plastic film, simultaneously completing cooling and agar rolling during the continuous rotation of the test tube. The generated water pressure can compress the waterproof plastic film to make it adhere tightly to the test tube wall, ensuring heat exchange and cooling efficiency, and also damping and slowing down the rotation of the test tube, keeping the test tube in an ultra-low speed rotation state suitable for rolling the sample, thus improving the forming quality and uniformity of the agar layer; during the rotation of the turntable, the external drive component synchronously drives the top gear and the distribution box to rotate with the turntable, ensuring that the relative positions of the water supply hose and the cooling box are always matched, avoiding pipe entanglement; the water supply hose has an integrated inlet pipe and return pipe, which can realize the circulation of cooling water and ensure the continuous and stable operation of the cooling system.

[0011] This invention provides an improved batch culture device for microbial detection, which, compared with the prior art, has the following improvements and advantages: 1. When using this invention, researchers only need to complete the inoculation of a single test tube with bacteria through the glove operation window on both sides of the box and place it in the corresponding work position of the rotating structure. The rotating structure will then drive the test tube to continuously flow along the preset path. During the process, it will automatically complete all the rolling sample-taking procedures such as inverting and shaking to mix the materials and continuous rolling during the cooling stage, without the need for manual intervention in the subsequent processing. 2. The vacant new workstation will be moved synchronously to the operator's work area along with the rotating structure, allowing researchers to immediately place the next inoculated test tube, realizing a continuous operation mode. There is no need to wait for the previous test tube to complete all the processes before starting the next operation. This not only greatly reduces the overall preparation time of a single batch of samples and significantly improves the efficiency of batch rolling tube operation, but more importantly, it completely eliminates the time interval differences caused by manual processing of each tube. This allows all test tubes to complete cell mixing, agar spreading, and cooling and solidification within a similar time window, ensuring a high degree of uniformity in the thickness of the agar layer and the spatial distribution of the cells in each test tube. This avoids problems such as premature agar solidification and uneven molding quality caused by misaligned sample preparation time, effectively improving the parallelism of growth and culture accuracy of batch rolling tube samples. Attached Figure Description

[0012] The present invention will be further explained below with reference to the accompanying drawings and embodiments: Figure 1 This is a three-dimensional structural schematic diagram of the present invention; Figure 2 This is a schematic diagram of the drive gear ring structure of the present invention; Figure 3 This is a schematic diagram of the column structure of the present invention; Figure 4 This is the present invention. Figure 3 Enlarged schematic diagram of part A; Figure 5 This is a schematic diagram of the curved ring structure of the present invention; Figure 6 This is a schematic diagram of the flow divider structure of the present invention; Figure 7 This is the present invention. Figure 6 Enlarged schematic diagram of section B structure; Figure 8 This is the present invention. Figure 7 Enlarged schematic diagram of section C; Figure 9 This is a schematic diagram of the water delivery hose structure of the present invention; Figure 10 This is the present invention. Figure 9 An enlarged schematic diagram of the D-section structure.

[0013] Explanation of reference numerals in the attached figures: 1. Main housing; 2. Base; 3. Mounting cylinder; 4. Turntable; 5. Connecting rod; 6. Cooling box; 7. Waterproof plastic film; 8. Driven gear; 9. Elastic rubber block; 10. Drive motor; 11. Drive gear; 12. Drive gear ring; 13. Support rod; 14. Curved ring; 15. Mounting bracket; 16. Slide rod; 17. Movable rack; 18. Spring 1; 19. Flip gear ring; 20. Column; 21. Fixed gear ring; 22. Vertical shaft; 23. Linkage gear; 24. Fixed friction disc; 25. C-shaped frame; 26. Sleeve; 27. Sleeve rod; 28. Movable friction disc; 29. ​​Spring 2; 30. Bevel gear 1; 31. Vertical rod; 32. Bevel gear 2; 33. Drive gear; 34. Diverter box; 35. Top gear; 36. Water supply hose; 37. Combination pipe; 38. Test tube; 39. Sealing plug. Detailed Implementation

[0014] The present invention will now be described in detail, and the technical solutions in the embodiments of the present invention will be clearly and completely described. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0015] This invention provides an improved batch culture device for microbial detection. The technical solution of this invention is as follows: like Figures 1-10 As shown, a batch culture device for microbial detection includes a main chamber 1. A base 2 is fixedly installed at the bottom of the inner cavity of the main chamber 1. A mounting cylinder 3 is rotatably connected to the top of the base 2. A turntable 4 is fixedly installed on the outer circumference of the mounting cylinder 3. Connecting rods 5 are rotatably connected to several crossbars arranged at equal angles on the outer circumference of the turntable 4. A cooling box 6 is welded to the side of each connecting rod 5 away from the turntable 4. A waterproof plastic film 7 is provided on the inner circumference of each cooling box 6. Driven gears 8 are rotatably connected to the upper and lower ends of each cooling box 6. Several crossbars 7 are arranged at equal angles on the inner circumference of each driven gear 8. The elastic rubber block 9 is used so that, during use, researchers can take the inoculum from the top shelf through the glove operation window on both sides of the box and inject it into the test tube 38 pre-filled with culture medium through the inoculator. After inoculation, the sealing plug 39 is fixed to the top opening of the test tube 38 to achieve a seal. Then, the test tube 38 is placed in the clamping position inside the cooling box 6. The inner circular surface of the driven gear 8 is provided with an elastic rubber block 9. The elastic rubber block 9 includes an elastic element located in the mounting groove on the inner circular surface of the driven gear 8 and a rubber clamp that abuts against the wall of the test tube 38. The test tube 38 can be stably clamped and fixed by the pre-tightening elastic force of the elastic rubber block 9.

[0016] Furthermore, a drive motor 10 is fixedly installed on the top of the base 2, and a drive gear 11 is fixedly installed on the top output end of the drive motor 10. A drive gear ring 12 that meshes with the drive gear 11 is fixedly sleeved on the outer circumference of the mounting cylinder 3. The drive motor 10 outputs power to drive the drive gear 11 to rotate at a low speed. Through gear meshing transmission, the drive gear ring 12, the mounting cylinder 3, and the turntable 4 rotate synchronously, and then drive the cooling box 6 and the test tube 38 to revolve along the circumferential path via the connecting rod 5. Several support rods 13 are fixedly installed at equal angles on the top of the base 2, and curved rings 14 are fixedly installed between the tops of the support rods 13. Several mounting brackets 15 are welded at equal angles on the bottom of the turntable 4. Each mounting bracket 15 has a side Sliding rods 16 are slidably installed on the wall. Each sliding rod 16 has a movable rack 17 welded to the side near the curved ring 14. A spring 18 is wound around the body of each sliding rod 16, and several springs 18 are respectively connected between the corresponding movable rack 17 and the mounting bracket 15. A flipping gear ring 19 that meshes with the corresponding movable rack 17 is welded to each connecting rod 5. A curved ring 14 with multiple protrusions is set along the revolution path of the test tube 38. When the test tube 38 revolves with the turntable 4 to the protrusion position of the curved ring 14, the movable rack 17 slides outward along the protrusion trajectory, driving the flipping gear ring 19 and the connecting rod 5 to rotate, thereby driving the cooling box 6 and the test tube 38 to complete a ninety-degree rotation.

[0017] Furthermore, a column 20 is welded to the center of the top of the base 2. A fixed gear ring 21 is welded to the outer surface of the column 20 via several horizontal bars arranged at equal angles. Several vertical shafts 22 are rotatably connected to the top of the turntable 4 at equal angles. Each vertical shaft 22 has a fixedly fitted linkage gear 23 that meshes with the fixed gear ring 21. A fixed friction disc 24 is fixedly installed on the top of each vertical shaft 22. The vertical shafts 22 and linkage gears 23 on the turntable 4 rotate synchronously with the turntable 4. Because the linkage gear 23 meshes with the fixed gear ring 21… The rings 21 mesh, and the linkage gear 23 rotates simultaneously during its revolution. A C-shaped frame 25 is welded to the back of each cooling box. A sleeve 26 is rotatably connected to the side wall of each C-shaped frame 25. A sleeve rod 27 is slidably disposed inside each sleeve 26. A movable friction disc 28 is fixedly installed on the side of each sleeve rod 27 away from the corresponding sleeve 26. A spring 29 is wound around the body of each sleeve rod 27, and several springs 29 are respectively located between the corresponding movable friction disc 28 and the corresponding sleeve 26. Each sleeve 26... A bevel gear 30 is fixedly installed on the side away from the corresponding sleeve rod 27. A vertical rod 31 is rotatably connected to each connecting rod 5. A bevel gear 32 that meshes with the bevel gear 30 is fixedly installed on the body of each vertical rod 31. Two symmetrical driving gears 33 are fixedly installed at the upper and lower ends of each vertical rod 31, and several driving gears 33 mesh with corresponding driven gears 8. After the test tube 38 is flipped to a horizontal state, the movable friction disk 28 abuts against the fixed friction disk 24. The rotation of the fixed friction disk 24... The force is transmitted through the movable friction disc 28 to the sleeve rod 27 and sleeve 26, and then through the reversing transmission of bevel gear 1 30 and bevel gear 2 32, it drives the vertical rod 31 and the driving gear 33 to rotate synchronously. The driving gear 33 meshes and drives the driven gear 8 to rotate, and finally drives the test tube 38 to complete a short-term rotation through the elastic rubber block 9. When the test tube 38 passes through multiple sets of protruding structures, it can cycle through the inverting and shaking and short-term rotation actions, realizing the combined effect of inverting and mixing and rotating and homogenizing, so that the bacterial solution and the molten culture medium are mixed more evenly and fully.

[0018] Furthermore, a distribution box 34 is rotatably connected to the top of the column 20. A top gear 35 is fixedly installed on the top of the distribution box 34. Water supply hoses 36 connect the distribution box 34 to several cooling boxes 6. A manifold 37 is rotatably connected to the top of the distribution box 34. When the test tube 38 revolves to the continuous protruding area of ​​the curved ring 14, the test tube 38 maintains a 90-degree rotation state and continues to rotate. At the same time, the external cooling pipe delivers cooling water to the inside of the distribution box 34 through the manifold 37. The electrically controlled valve corresponding to the water supply hose 36 in the distribution box 34 opens, and the cooling water enters the cooling box 6 through the water supply hose 36. It fully contacts the outer wall of the test tube 38 through the waterproof plastic film 7, and is simultaneously completed during the continuous rotation of the test tube 38. The cooling system facilitates cooling and agar rolling. The water pressure generated by the cooling water can compress the waterproof plastic film 7 to ensure it adheres tightly to the wall of the test tube 38, thus guaranteeing heat exchange and cooling efficiency while also damping and slowing down the rotation of the test tube 38. This maintains the test tube 38 in an ultra-low speed rotation state suitable for rolling the sample, improving the forming quality and uniformity of the agar thin layer. During the rotation of the turntable 4, the external drive component synchronously drives the top gear 35 and the distribution box 34 to rotate together with the turntable 4, ensuring that the relative positions of the water supply hose 36 and the cooling box 6 are always matched, preventing the pipes from tangling. The manifold 37 and the water supply hose 36 are internally equipped with inlet and return pipes, which can realize the circulation of cooling water and ensure the continuous and stable operation of the cooling system.

[0019] Working principle: During use, researchers take the inoculum from the top shelf through the glove operation windows on both sides of the box and inject it into the test tube 38 pre-filled with culture medium through the inoculator. After inoculation, the sealing plug 39 is fixed to the top opening of the test tube 38 to achieve a seal. Then, the test tube 38 is placed in the clamping position inside the cooling box 6. The inner circular surface of the driven gear 8 is provided with an elastic rubber block 9. The elastic rubber block 9 includes an elastic element located in the mounting groove on the inner circular surface of the driven gear 8 and a rubber clamp that abuts against the wall of the test tube 38. The test tube 38 can be stably clamped and fixed by the pre-tightening elastic force of the elastic rubber block 9. The drive motor 10 outputs power to drive the drive gear 11 to rotate at low speed. Through gear meshing, it drives the drive gear ring 12, mounting cylinder 3, and turntable 4 to rotate synchronously. This, in turn, drives the cooling box 6 and test tube 38 to revolve along a circular path via the connecting rod 5. A curved ring 14 with multiple protrusions is provided along the revolving path of the test tube 38. When the test tube 38 revolves with the turntable 4 to the protruding position of the curved ring 14, the movable rack 17 slides outward along the protruding trajectory, driving the rotating gear ring 19 and the connecting rod 5 to rotate, thereby driving the cooling box 6 and test tube 38 to complete a 90-degree rotation. The vertical shaft 22 and the linkage gear 23 on the turntable 4 rotate synchronously with the turntable 4. During the revolution, the linkage gear 23 meshes with the fixed gear ring 21, causing the linkage gear 23 to rotate simultaneously during the revolution. After the test tube 38 flips to a horizontal position, the movable friction disk 28 abuts against the fixed friction disk 24. The rotational power of the fixed friction disk 24 is transmitted through the movable friction disk 28 to the sleeve rod 27 and the sleeve 26. Then, through the reversing transmission of the bevel gear 1 30 and bevel gear 2 32, the vertical rod 31 and the driving gear 33 rotate synchronously. The driving gear 33 meshes and drives the driven gear 8 to rotate. Finally, the elastic rubber block 9 drives the test tube 38 to complete a short-term rotation. The test tube 38 can cycle through multiple sets of protruding structures. The inverted shaking and short-term rotation achieve a combined effect of inverted mixing and rotational homogenization, ensuring a more uniform and thorough mixing of the bacterial solution and the molten culture medium. When test tube 38 rotates to the continuous raised area of ​​the curved ring 14, test tube 38 maintains a 90-degree inverted state and continues to rotate. Simultaneously, the external cooling pipe delivers cooling water to the distribution box 34 through the manifold 37. The electrically controlled valve corresponding to the water delivery hose 36 in the distribution box 34 opens, allowing cooling water to flow into the cooling box 6 through the water delivery hose 36. The cooling water then fully contacts the outer wall of test tube 38 through the waterproof plastic film 7, simultaneously completing cooling and agar rolling during the continuous rotation of test tube 38. Cooling water generation... The water pressure can squeeze the waterproof plastic film 7 to make it tightly adhere to the wall of the test tube 38, which not only ensures the heat exchange and cooling efficiency, but also creates a damping and deceleration effect on the rotation of the test tube 38, so that the test tube 38 maintains an ultra-low speed rotation state suitable for rolling the sample, improving the forming quality and uniformity of the agar thin layer; during the rotation of the turntable 4, the external drive component synchronously drives the top gear 35 and the distribution box 34 to rotate together with the turntable 4, ensuring that the relative position of the water supply hose 36 and the cooling box 6 is always matched, and avoiding the pipes from getting tangled; the manifold 37 and the water supply hose 36 are internally equipped with inlet and return pipes, which can realize the circulation of cooling water and ensure the continuous and stable operation of the cooling system; The vacant new workstation is simultaneously moved to the operator's work area by the rotating structure, allowing researchers to immediately place the next inoculated test tube 38, realizing a continuous operation mode. There is no need to wait for the previous test tube 38 to complete all the processes before starting the next operation. This not only greatly reduces the overall preparation time of a single batch of samples and significantly improves the efficiency of batch rolling tube operation, but more importantly, it completely eliminates the time interval differences caused by manual processing of each tube. This allows all test tubes 38 to complete cell mixing, agar spreading, and cooling and solidification within a similar time window, ensuring a high degree of uniformity in the thickness of the agar layer and the spatial distribution of the cells in each test tube 38. It avoids problems such as premature agar solidification and uneven molding quality caused by misalignment of sample preparation time, effectively improving the parallelism of growth and culture accuracy of batch rolling tube samples.

[0020] The foregoing description enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A batch culture device for microbial detection, comprising a main chamber (1), characterized in that: A base (2) is fixedly installed at the bottom of the inner cavity of the main box (1). An installation cylinder (3) is rotatably connected to the top of the base (2). A turntable (4) is fixedly installed on the outer circular surface of the installation cylinder (3). A connecting rod (5) is rotatably connected to several horizontal bars arranged at equal angles on the outer circular surface of the turntable (4). A cooling box (6) is welded to the side of each connecting rod (5) away from the turntable (4). A waterproof plastic film (7) is provided on the inner circular surface of each cooling box (6). A driven gear (8) is rotatably connected to the upper and lower ends of each cooling box (6). Several elastic rubber blocks (9) are arranged at equal angles on the inner circular surface of each driven gear (8).

2. The batch culture device for microbial detection according to claim 1, characterized in that: The base (2) is fixedly mounted with a drive motor (10) on the top, and a drive gear (11) is fixedly mounted on the top output end of the drive motor (10). A drive gear ring (12) that meshes with the drive gear (11) is fixedly sleeved on the outer circumference of the mounting cylinder (3).

3. The batch culture device for microbial detection according to claim 1, characterized in that: The base (2) has several support rods (13) fixedly installed at equal angles on its top. A curved ring (14) is fixedly installed between the tops of the support rods (13). The turntable (4) has several mounting brackets (15) welded at equal angles on its bottom. Each mounting bracket (15) has a sliding rod (16) slidably installed on its side wall. Each sliding rod (16) has a movable rack (17) welded on its side near the curved ring (14). Each sliding rod (16) has a spring (18) wound around its body. Several springs (18) are connected between the corresponding movable rack (17) and the mounting bracket (15). Each connecting rod (5) has a rotating toothed ring (19) that meshes with the corresponding movable rack (17).

4. The batch culture device for microbial detection according to claim 1, characterized in that: The base (2) has a column (20) welded to the top center. The column (20) has a fixed gear ring (21) welded to the outer surface of the column (20) by several crossbars arranged at equal angles. The turntable (4) has several vertical shafts (22) rotatably connected to the top at equal angles. Each vertical shaft (22) has a linkage gear (23) fixedly sleeved on its shaft body that meshes with the fixed gear ring (21). Each vertical shaft (22) has a fixed friction disc (24) fixedly installed on its top.

5. The batch culture device for microbial detection according to claim 1, characterized in that: Each cooling box has a C-shaped frame (25) welded to its back. Each C-shaped frame (25) has a sleeve (26) rotatably connected to its side wall. Each sleeve (26) has a sliding rod (27) inside it. Each sleeve (27) has a movable friction disc (28) fixedly installed on the side away from the corresponding sleeve (26). Each sleeve (27) has a spring (29) wound around its body. Several springs (29) are located between the corresponding movable friction disc (28) and the corresponding sleeve (26). Each sleeve (26) has a bevel gear (30) fixedly installed on the side away from the corresponding sleeve (27).

6. The batch culture device for microbial detection according to claim 5, characterized in that: Each of the connecting rods (5) is rotatably connected to a vertical rod (31), and each of the vertical rods (31) is fixedly installed with a bevel gear (32) that meshes with bevel gear one (30). Each of the vertical rods (31) has two symmetrical driving gears (33) fixedly installed at its upper and lower ends, and several driving gears (33) mesh with corresponding driven gears (8).

7. The batch culture device for microbial detection according to claim 4, characterized in that: The top of the column (20) is rotatably connected to a distribution box (34), and a top gear (35) is fixedly installed on the top of the distribution box (34). Water supply hoses (36) are connected between the distribution box (34) and several cooling boxes (6). A collection pipe (37) is rotatably connected to the top of the distribution box (34).

Images