A high-quality maa-producing starrensispora bred by gamma ray mutagenesis and use thereof

Abstract

The application discloses a high-quality MAA-producing Asterarcys sp. obtained through gamma ray mutagenesis breeding and application thereof. Asterarcys sp. SCSIO-46858 has been preserved in the China Center for Type Culture Collection (CCTCC) on April 8, 2026; the address is Luojiashan, Wuchang, Wuhan, Hubei, China, and the postcode is 430072; and the strain preservation number is CCTCC NO M 2026630. The application provides a potential Asterarcys sp. production strain, and the alga can be used in stable and high production of MAA (spore amino acid), synchronous accumulation of oil and unsaturated fatty acid, and application in fields of MAAs-containing sunscreen cosmetics, functional food, medical adjuvant and biofuel and the like.

Description

Technical Field

[0001] This invention belongs to the field of microalgae, specifically relating to a high-quality MAAs-producing algae, Asterarcys sp. SCSIO-46858, obtained through gamma-ray mutagenesis breeding, and its uses. Background Technology

[0002] Mycosporine-like amino acids (MAAs) are a class of water-soluble secondary metabolites produced by aquatic organisms to protect against ultraviolet radiation or cope with environmental stress. These substances can efficiently absorb ultraviolet light and have a high molar extinction coefficient. Unlike traditional chemical sunscreens which have potential toxicity, MAAs can safely dissipate absorbed ultraviolet energy as heat, a process that does not produce photoreaction byproducts or reactive oxygen species, thus avoiding photodamage to the skin. Furthermore, MAAs exhibit good stability over a wide temperature and pH range and possess various biological activities such as antioxidant, anti-inflammatory, anti-tumor, and anti-aging properties. Therefore, MAAs, as a safe, environmentally friendly natural sunscreen ingredient with both high-efficiency ultraviolet absorption and antioxidant functions, have broad market prospects.

[0003] Currently, there are representative MAAs-based products on the market, including HeliGuard™ 365 (derived from Porphyra yezoensis extract, containing porphyra-334 and shinorine) launched by Mibelle AG Biochemistry in Switzerland, and HELINORI® (also derived from Porphyra yezoensis, with palythine as the active ingredient) launched by Gelyma in France.

[0004] However, the aforementioned products have the following significant drawbacks: 1) Raw material dependence on macroalgae. Their MAAs raw materials are all extracted directly from macroalgae (such as *Porphyra yezoensis*), but macroalgae have long growth cycles and low biomass yields, making rapid and large-scale raw material supply difficult. 2) Limited MAAs content. The natural accumulation level of MAAs in macroalgae is low, resulting in low extraction efficiency and a low product yield per unit algal cell. 3) High production costs. Due to the slow growth and low content of red algae, the MAAs extraction process is complex and energy-intensive, leading to high final product prices. 4) Limited market promotion. The high cost severely restricts the application of MAAs products in the mass market, currently limiting their positioning to the high-end skincare sector and failing to meet the growing market demand for natural sunscreens.

[0005] To overcome the industrialization bottleneck of microalgae-derived MAAs, domestic enterprises and research institutions are currently focusing on two main directions: first, inducing microalgae to synthesize MAAs by optimizing cultivation conditions (such as ultraviolet radiation and salinity stress) to increase yield; and second, developing processes for directly extracting MAAs from macroalgae to develop high-value-added sunscreen products. However, significant breakthroughs in this field have not yet been achieved domestically, and no company has yet achieved large-scale MAAs production; most research remains at the laboratory pilot stage. The core technological bottlenecks in microalgae-derived MAAs production are mainly reflected in the following two aspects: first, the lack of high-performance engineered algal strains that can stably produce high yields of MAAs under conventional cultivation conditions; and second, existing induction processes rely heavily on costly environmental stressors (such as strong light, ultraviolet radiation, and salinity stress), making them unsuitable for large-scale cultivation scenarios. A low-cost, high-efficiency MAAs induction process suitable for engineered cultivation has not yet been established.

[0006] Microalgae can accumulate various bioactive substances, among which mycospore-like amino acids (MAAs), as natural UV absorbers and antioxidants, have attracted much attention in recent years. Currently, the MAAs-producing algae discovered domestically and internationally are mainly large red algae. Although these algae possess the ability to synthesize MAAs, they generally suffer from slow growth rates, weak environmental adaptability, low yields of the target product, and high cultivation costs. Among microalgae, cyanobacteria are the main MAAs-producing species, which also face problems such as low MAAs content and slow growth rates. Furthermore, only a few green algae have been reported to have the potential to synthesize MAAs, and under conventional cultivation conditions, they usually do not produce them or produce them in extremely low quantities. Therefore, the cultivation cost of producing MAAs using existing microalgae is high, making large-scale application difficult. The main reasons include the following:

[0007] 1. Microalgae produce extremely low basic yields of MAAs under conventional culture conditions. Even with induction methods such as ultraviolet radiation and salinity adjustments, the accumulation level of MAAs is still insufficient to meet the economic requirements for large-scale extraction. To address this issue, the *Star Net Algae* strain provided in this patent possesses a stable and high-yield capacity for MAAs, enabling the large-scale synthesis of MAAs without complex or demanding induction conditions. This provides a high-quality algal strain resource for the industrial production and application of MAAs.

[0008] 2. Traditional MAAs-producing algal strains generally suffer from slow growth and sensitivity to environmental fluctuations, resulting in low cultivation efficiency and high costs. The *Star Net Algae* strain provided in this patent possesses strong environmental adaptability, rapid growth capacity, and excellent tolerance to industrial flue gas. It can directly utilize industrial flue gas for growth, achieving biomass value-added while contributing to carbon emission reduction, thereby significantly reducing cultivation costs and improving overall process efficiency.

[0009] Gamma-ray mutagenesis breeding of microalgae is a highly efficient artificial breeding technology based on high-energy physical radiation. It utilizes the strong penetrating power and high energy of gamma rays to directly act on the genetic material of microalgal cells, inducing DNA breakage and misalignment, thereby generating rich genetic variations through the cell's own repair mechanisms. This method can obtain a large-scale mutation library in a short time and rapidly obtain superior algal strains with excellent growth performance, high content of target products, and genetic stability through targeted screening. Compared with traditional breeding, gamma-ray mutagenesis has the following outstanding advantages: high mutagenesis rate, broad mutation spectrum, and short breeding cycle; radiation conditions (such as dose and dose rate) can be precisely controlled, with good reproducibility; it does not rely on special environments (such as space conditions), can be carried out on a large scale in the laboratory, is simple to operate, and has controllable costs, making it more suitable for germplasm creation in the early stages of industrialization. The *Star Net Algae* strain provided in this patent was obtained through gamma-ray irradiation mutagenesis and multiple rounds of high-throughput screening. This algal strain not only has a significantly improved growth rate but also exhibits good tolerance to industrial flue gas, maintaining stable growth under culture conditions containing typical flue gas components. At the same time, the content of high-value-added products such as cytosine amino acids and oils in its body has also increased significantly, showing strong resistance, good product diversity and excellent comprehensive performance, and industrial development potential.

[0010] Among the currently published patents related to *Star-Net Algae*, no patents were found concerning the protection of *Star-Net Algae* strains with high MAAs production. The relevant technologies retrieved mainly focus on the following aspects: increasing the MAAs content in macroalgae by changing culture conditions, producing MAAs through metabolic engineering of heterotrophic microorganisms, and isolating and purifying MAAs from red algae. Additionally, some patents relate to the lipid accumulation characteristics of certain *Star-Net Algae* strains but do not involve MAAs production. Representative patents are as follows:

[0011] 1. A cultivation method for increasing MAAs content in *Porphyra yezoensis* (202311123456.7). This invention increases the MAAs content in *Porphyra yezoensis* by sterilization with 254nm ultraviolet light, seawater cultivation under 80 mmol salt stress, and continuous irradiation cultivation using multiple light sources consisting of ultraviolet lamps, violet lamps, green lamps, yellow lamps, and blue lamps. This technology relies on salt stress and multi-light source induction, and uses large red algae as raw material. It is limited by the long growth cycle of red algae and the high cost of multi-light source induction, making large-scale production difficult.

[0012] 2. MysDs enzyme for the specific production of mycospore-like amino acids and its preparation method and process (202411226172.5). This technology involves the production of MAAs through metabolic engineering of microorganisms, introducing genes such as mysA, mysB, mysC, and mysD to produce various types of MAAs. This technology uses heterotrophic microbial hosts, which differs from the photosynthetic autotrophic microalgae route of this invention, and involves genetic engineering modification, thus facing regulatory obstacles for industrial application.

[0013] 3. A method for isolating and purifying porphyra-334, a mycotoxin-like amino acid, from Gracilaria (202210434425.8). This invention discloses a method for isolating and purifying porphyra-334 from Gracilaria to fill the supply gap of this MAA standard both domestically and internationally. However, this technology belongs to the separation and purification process, and the raw material still relies on large red algae. Limited by the long growth cycle of red algae and the low natural content of MAAs, it cannot solve the raw material supply bottleneck at its source.

[0014] 4. A strain of *Stellaria media* and its cultivation method and application (CN201610138113.7). This invention discloses a strain of *Stellaria media* HCS-02 that can tolerate low cultivation temperatures and exhibits a relatively fast biomass accumulation rate under different cultivation conditions. The obtained biomass is rich in starch and oil. However, although this strain has the ability to accumulate oil, it does not involve the production of macro-ascorbic acid (MAAs), and cannot achieve multi-product co-production of MAAs and oils, resulting in limited overall economic benefits.

[0015] Analysis of the aforementioned patents revealed no patents protecting microalgae strains that provide stable, high-yield MAAs and exhibit resistance to flue gas and lipid accumulation. Regarding increasing MAAs yield, publicly available technologies primarily rely on altering culture conditions (such as salt stress or multi-source induction) or extraction from large red algae. These technologies are limited by the long growth cycle of red algae, high induction costs, and unstable raw material supply, making large-scale scaling difficult. Furthermore, the technology route based on metabolic engineering to produce MAAs faces regulatory hurdles regarding the outdoor cultivation of genetically engineered strains, thus lacking industrial feasibility.

[0016] Currently, the field of microalgae-based MAAs production mainly suffers from the following three technical deficiencies:

[0017] (1) The existing MAAs-producing algal strains have extremely low basic yields of MAAs under conventional culture conditions. Even with the application of induction methods such as ultraviolet radiation and salinity, their accumulation level is still difficult to meet the requirements of large-scale extraction processes for raw material quality, which hinders the development of deep-processed products.

[0018] (2) Existing MAAs-producing algal strains have long growth cycles and low biomass and MAAs yield per unit time, which limits the economic benefits of large-scale production.

[0019] (3) Existing MAAs producing algae strains are sensitive to high temperature, flue gas and other environments, and cannot be directly cultivated at low cost using industrial waste heat or industrial flue gas. Furthermore, it is difficult to achieve the co-production of MAAs and multiple products such as oils, resulting in high cultivation costs. At the same time, they are easily contaminated by other algae, affecting product stability. Summary of the Invention

[0020] This invention provides a strain of Asterarcys sp. SCSIO-46858 obtained through cobalt-60 ray mutagenesis. The Latin name of this microalga is Asterarcys sp. SCSIO-46858. It was successfully deposited on April 8, 2026, at the China Center for Type Culture Collection (CCTCC); address: Luojia Mountain, Wuchang, Wuhan, Hubei Province, 430072, China; strain accession number: CCTCC NO: M2026630.

[0021] This invention provides a strain of Asterarcys sp. SCSIO-46858, bred through gamma-ray mutagenesis. This strain has the characteristics of fast growth rate, high production of MAAs, strong resistance to flue gas, and high oil accumulation capacity. It can be applied to the production of high-quality MAAs raw materials, co-production of microalgal oil from industrial flue gas carbon capture, and multi-product biorefining, and has high development and utilization potential.

[0022] The culture conditions for the *Asterarcys* sp. strain SCSIO-46858 of this patent involve the addition of five elements: carbon, nitrogen, phosphorus, iron, and sulfur. Nitrogen can be sodium nitrate, potassium nitrate, sodium nitrite, urea, ammonium bicarbonate, ammonium carbonate, ammonia, ammonium chloride, etc., at a concentration of 0.1 mM-20 mM; carbon can be carbon dioxide, sodium carbonate, and sodium bicarbonate, at a concentration of 0.1 mM-200 mM; phosphorus can be sodium dihydrogen phosphate, potassium dihydrogen phosphate, disodium hydrogen phosphate, dipotassium hydrogen phosphate, phosphoric acid, etc., at a concentration of 0.01 mM-20 mM; iron can be ferric ammonium citrate and ferric chloride, at a concentration of 10 μM-50 μM; and sulfur can be sodium sulfite, potassium sulfate, etc., at a concentration of 0.02 mM-20 mM.

[0023] The morphological characteristics of the Asterarcys sp. strain SCSIO-46858 in this patent are as follows: the cells are spherical to ellipsoidal, with a starch nucleus and obvious pyrenoid-amyloid plates, often forming coenobia-like structures encased in a gelatinous substance. Young cells exhibit meridional ridges and an asymmetrical spindle-shaped structure with multiple or single-pointed tips. Each cell is approximately 11.5 micrometers long and 8.5 micrometers wide. Mature cells are subspherical, with a cell length of approximately 19.8 micrometers and a width of approximately 18.1 micrometers.

[0024] The biomass of the patented Asterarcys sp. strain SCSIO-46858 can reach 1.65 g / L under normal culture conditions, which can be used for large-scale production and reduce the cost of spirulina cultivation.

[0025] Therefore, a second objective of the present invention is to provide the application of the above-mentioned Star-Net Algae SCSIO-46858 in the preparation of microalgae feed.

[0026] The MAAs content of this patented Asterarcys sp. strain SCSIO-46858 can reach 3.08 g / 100 g under moderate phosphorus restriction and daily addition of small amounts of phosphorus, and can be used for MAAs extraction.

[0027] Therefore, a third objective of this invention is to provide the application of the above-mentioned Star-Net Algae SCSIO-46858 in the preparation of Mycosporine-like amino acids (MAAs).

[0028] The patented Asterarcys sp. strain SCSIO-46858 can achieve an oil content of 42.53 g / 100 g under moderate phosphorus restriction and daily addition of small amounts of phosphorus, and can be used for oil extraction.

[0029] Therefore, a fourth object of the present invention is to provide the application of the above-mentioned Star-Net Algae SCSIO-46858 in the preparation of oils and / or unsaturated fatty acids.

[0030] This patented strain of *Asterarcys* sp., SCSIO-46858, can tolerate 20 mM SO3. 2- and 16 mM NO2 - 9 hours of incubation can remove 1.93 mM SO3. 2- 120 h of culture can remove 1.89 mM NO2. - .

[0031] Therefore, a fourth object of the present invention is to provide the above-mentioned Star-Net Algae SCSIO-46858 in removing SO3 2- and / or NO2 - Applications in [the field].

[0032] This patented Star Net Algae strain contains high levels of mycospore-like amino acids (MAAs), oils, and unsaturated fatty acids, and can be used in sunscreen cosmetics, functional foods, pharmaceutical excipients, biofuels, and feed additives.

[0033] Therefore, a fifth object of the present invention is to provide the application of the above-mentioned Star-Net Algae SCSIO-46858 in the preparation of sunscreen cosmetics, functional foods, pharmaceutical excipients, biofuels and / or feed additives.

[0034] This patented strain of Asterarcys sp. (SCSIO-46858) can be harvested at low cost through natural settling.

[0035] This patented strain of Asterarcys sp. (SCSIO-46858) can be cultivated under both outdoor and indoor conditions, with a light intensity of 50-5000 μmol photons / m². 2 s.

[0036] The sixth objective of this invention is to provide a method for culturing Star-Net Algae SCSIO-46858, wherein Star-Net Algae SCSIO-46858 is cultured in a culture medium under light conditions.

[0037] Preferably, the culture medium is a microalgae-compatible medium, such as BG-11 liquid medium or phosphorus-free ZSNT medium. The phosphorus-free ZSNT medium comprises: 5.0 g / L NaHCO3, 0.7 g / L NaNO2, 0.005 g / L FeCl3·7H2O, 0.4 g / L Na2SO3, 0.1 g / L KCl, 0.02 g / L CaCl2, 0.05 g / L MgSO4·7H2O, and 1 mL / L A5, with water as the solvent. A5 consists of: 2.86 g / L H3BO3, 1.81 g / L MnCl2·4H2O, 0.22 g / L ZnSO4·7H2O, 0.39 g / L Na2MoO4·2H2O, and 0.08 g / L CuSO4·5H2O. and 0.05 g / L Co(NO3)2·6H2O, with water as the solvent.

[0038] Preferably, the culture is carried out under a light intensity of 200 μmol photons / m²·s and a temperature of 25℃.

[0039] Preferably, the cultivation is carried out using industrial flue gas.

[0040] The present invention provides a *Star Net Algae* strain screened by gamma-ray mutagenesis, which has the following advantages:

[0041] (1) The algal strain provided by the present invention can stably produce high MAAs. By controlling the nitrogen and phosphorus in the culture medium, the MAAs content of the algal strain can be greatly increased, which can meet the requirements of the subsequent extraction process for MAAs content and provide high-quality algal strain resources for the production of high-quality MAAs raw materials.

[0042] (2) The algal strain provided by the present invention can still maintain efficient MAAs synthesis and lipid accumulation under phosphorus-limited culture conditions, and has good adaptability to low phosphorus environment. Thus, it can achieve efficient production of target products and co-production of lipids without pursuing high growth rate, and significantly reduce the cost of phosphate fertilizer input.

[0043] (3) The algal strain provided by this invention has excellent resistance to flue gas and can be directly used in industrial flue gas (containing high concentrations of CO2 and SO2). x NO x Cultivating (etc.) can significantly reduce carbon source costs and temperature control energy consumption.

[0044] Producing MAAs alone is often not economically viable, limiting their large-scale application. This patented *Stellaria media* enables the co-production of oils (which can be used for biodiesel, etc.) while producing high-value MAAs. By maximizing the value of biomass through multiple product outputs and reducing overall costs, it creates a significant cost and benefit advantage in industrial applications.

[0045] This invention provides a promising *Asteris stellaria* strain for production, which can be used for the stable and high-yield production of MAAs (microbial spore-like amino acids), the simultaneous accumulation of oils and unsaturated fatty acids, and applications in the fields of MAAs-containing sunscreen cosmetics, functional foods, pharmaceutical excipients, and biofuels. For those skilled in the art, various improvements and modifications can be made to this invention without departing from its principles, and these improvements and modifications also fall within the scope of protection of the claims.

[0046] Asterarcys sp. SCSIO-46858 was deposited on April 8, 2026 at the China Center for Type Culture Collection (CCTCC); address: Luojia Mountain, Wuchang, Wuhan, Hubei Province, 430072, China; accession number: CCTCC NO M 2026630. Attached Figure Description

[0047] Figure 1 Cell morphology of the Star-Net Algae SCSIO-46858 strain;

[0048] Figure 2 NO2 of the Star-Net Algae SCSIO-46858 strain - Tolerance assessment;

[0049] Figure 3 The strain SCSIO-46858 of *Stellaria spp.* and the originating strain NO2 - Tolerance comparison;

[0050] Figure 4 SO3 of the Star-Net Algae SCSIO-46858 strain 2- Tolerance assessment;

[0051] Figure 5The strain SCSIO-46858 of *Stellaria spp.* and the originating strain SO3 2- Tolerance comparison;

[0052] Figure 6 Comparison of MAAs content between the *Star-Net Algae* SCSIO-46858 strain and the original strain;

[0053] Figure 7 Comparison of the lipids of the Star-Net Algae SCSIO-46858 strain and the original strain. Detailed Implementation

[0054] To enable those skilled in the art to better understand the technical solutions of the present invention, the present invention will be further described in detail below with reference to specific embodiments.

[0055] Example 1:

[0056] 1. Breeding and tolerance evaluation of high-quality Star-Net Algae SCSIO-46858 strain

[0057] 1) The starting algal strain, Asterarcys sp., was treated with gamma ray irradiation. After mutagenesis, the algal solution was cultured in the dark for 24 h. Then, it was spread on BG-11 solid plates or transferred to BG-11 liquid medium for enrichment culture.

[0058] 2) After appropriately diluting the mutagenized algal solution, spread it on BG-11 solid plates and incubate it in a light incubator for 10–15 days. Select single algal colonies with good growth and large colonies, transfer them to sterile 96-well plates, add BG-11 liquid medium and continue incubation.

[0059] 3) Periodically measure the OD of the algal solution in the 96-well plate using a microplate reader. 750 By measuring the absorbance of the characteristic absorption peak (323 nm) of mycospore-like amino acids (MAAs), candidate mutants with superior growth and MAAs accumulation were screened.

[0060] 4) The superior mutant strains obtained from the initial screening were transferred to 50 mL and 500 mL Erlenmeyer flasks for further propagation and screening. Their biomass concentration, MAAs content, oil content and flue gas tolerance were measured to comprehensively evaluate their traits.

[0061] 5) After 30 consecutive generations of subculture, a mutant strain of *Asterarcys* with stable MAAs production, high oil content, and excellent resistance to flue gas was obtained and named *Asterarcys sp. SCSIO-46858*. It was deposited on April 8, 2026, at the China Center for Type Culture Collection (CCTCC); address: Luojia Mountain, Wuchang, Wuhan, Hubei, 430072, China; accession number: CCTCC NO: M 2026630. The cell morphology of the *Asterarcys sp. SCSIO-46858* strain is as follows: Figure 1 As shown.

[0062] 2. Evaluation of the flue gas tolerance performance of Star-Net Algae SCSIO-46858

[0063] Asterarcys sp. SCSIO-46858 or the starting strain, *Asterarcys sp.*, was cultured on ZSNT medium with the following composition: 1.0 g / L NaHCO3, 0.5 g / L NaNO3, 0.005 g / L FeCl3·7H2O, 0.1 g / L KCl, 0.05 g / L Na2HPO4·2H2O, 0.02 g / L CaCl2, 0.05 g / L MgSO4·7H2O, and 1 mL / L A5, with water as the solvent. A5 consisted of: 2.86 g / L H3BO3, 1.81 g / L MnCl2·4H2O, 0.22 g / L ZnSO4·7H2O, 0.39 g / L Na2MoO4·2H2O, and 0.08 g / L CuSO4·5H2O. and 0.05 g / L Co(NO3)2·6H2O, with water as the solvent.

[0064] NO2⁻ Tolerance Assessment: NaNO₃ in ZSNT medium was replaced with 2, 4, 8, 16, and 32 mM NaNO₃, respectively. The control group used normal ZSNT medium (corresponding to 0 mM NaNO₃). Initial OD 750 The concentration was 0.1, and the culture conditions were: light intensity 200 μmol photons / m²·s, temperature 25℃, cultured for 7 days, and the growth status was observed. Figure 2 The highest NO2⁻ tolerance was 16 mM, significantly higher than that of the original algal strain, *Asterarcys* sp. Figure 3 ).

[0065] SO3²⁻ tolerance assessment: MgSO₄·7H₂O in ZSNT medium was replaced with 2, 5, 10, 20, 80, and 160 mM NaSO₃, respectively, and 0.2 mM MgCl₂ was supplemented to provide magnesium ions. The control group used normal ZSNT medium (corresponding to 0 mM NaSO₃). Culture conditions were: light intensity 200 μmol photons / m²·s, temperature 25℃, cultured for 7 days, and growth was observed. Figure 4 SO3 2 The highest tolerance level was 20 mM, significantly higher than that of the original algal strain, *Asterarcys* sp. Figure 5 ).

[0066] 3. Obtaining biomass from the *Star-Net Algae* SCSIO-46858 strain

[0067] Phosphorus-free ZSNT medium was used with *Asterarcys sp.* SCSIO-46858 or the starting strain *Asterarcys sp.*, with the following composition: 5.0 g / L NaHCO3, 0.7 g / L NaNO2, 0.005 g / L FeCl3·7H2O, 0.4 g / L Na2SO3, 0.1 g / L KCl, 0.02 g / L CaCl2, 0.05 g / L MgSO4·7H2O, and 1 mL / L A5, with water as the solvent. A5 consisted of: 2.86 g / L H3BO3, 1.81 g / L MnCl2·4H2O, 0.22 g / L ZnSO4·7H2O, 0.39 g / L Na2MoO4·2H2O, and 0.08 g / L CuSO4·5H2O. and 0.05 g / L Co(NO3)2·6H2O, with water as the solvent. 1 mg / L Na2HPO4·2H2O is added daily at a light intensity of 200 μmol photos / m². 2 s, initial inoculation OD 750 The concentration was 0.5, the culture temperature was 25℃, and the culture time was 7 days. Asterarcys sp. SCSIO-46858 achieved a biomass concentration of 1.65 g / L, a MAAs content of 3.08 g / 100 g, and a lipid content of 42.53 g / 100 g. The MAAs and lipid contents of Asterarcys sp. SCSIO-46858 were significantly better than the original strain (the existing strain shown in the figure). Figure 6 , 7 ).

[0068] The fatty acid composition and relative content of Asterarcys sp. SCSIO-46858 cultured for 7 days were analyzed (COHEN Z, NORMAN HA, HEIMER Y M. Potential use of substituted pyridazinones for selecting polyunsaturated fatty acid overproducing celllines of algae [J]. Phytochemistry, 1993, 32(2): 259-64.), as shown in Table 1. Although monounsaturated fatty acids (C18:1) and saturated fatty acids (C16:0) were the dominant components, polyunsaturated fatty acids (C18:2, C18:3) accounted for a total of 25.76%, of which α-linolenic acid (C18:3) content was as high as 15.21%, which was significantly higher than that of common microalgae, and has great application potential in the development of functional oils, health foods and aquatic feed.

[0069] Table 1

[0070] Fatty acid type Percentage (%) C16:0 28.94±0.22 C16:1 0.27±0.00 C18:0 3.74±0.12 C18:1 31.70±0.06 C18:2 10.55±0.06 C18:3 15.21±0.25 Other 9.58±0.13

Claims

1. Asterarcys sp. SCSIO-46858, accession number: CCTCC NO: M2026630.

2. The application of Star-Net Algae SCSIO-46858 as described in claim 1 in the preparation of microalgae feed.

3. The application of Star-Net Algae SCSIO-46858 as described in claim 1 in the preparation of mycosporin-like amino acids.

4. The use of Star-Net Algae SCSIO-46858 as described in claim 1 in the preparation of oils and / or unsaturated fatty acids.

5. The Star-Net Algae SCSIO-46858 as described in claim 1 in removing SO3 2- and / or NO2 - Applications in [the context of the text].

6. The use of Star-Net Algae SCSIO-46858 as described in claim 1 in the preparation of sunscreen cosmetics, functional foods, pharmaceutical excipients, biofuels and / or feed additives.

7. A method for cultivating Star-Net Algae SCSIO-46858, characterized in that, Star-shaped algae SCSIO-46858 were cultured in a culture medium under light conditions.

8. The cultivation method according to claim 7, characterized in that, The culture medium is BG-11 medium or phosphorus-free ZSNT medium. The phosphorus-free ZSNT medium consists of the following components: 5.0 g / L NaHCO3, 0.7 g / L NaNO2, 0.005 g / L FeCl3·7H2O, 0.4 g / L Na2SO3, 0.1 g / L KCl, 0.02 g / L CaCl2, 0.05 g / L MgSO4·7H2O, 1 mL / L A5, with water as the solvent. A5 consists of the following components: 2.86 g / L H3BO3, 1.81 g / L MnCl2·4H2O, 0.22 g / L ZnSO4·7H2O, 0.39 g / L Na2MoO4·2H2O, 0.08 g / L CuSO4·5H2O, and 0.05 g / L Co(NO3)2·6H2O, with water as the solvent.

9. The cultivation method according to claim 7, characterized in that, The culture was carried out under a light intensity of 50-5000 μmol photons / m². 2 s, temperature 25℃.

10. The cultivation method according to claim 7, characterized in that, The cultivation process utilizes industrial flue gas.

Images