A municipal sludge microbial conditioner, a preparation method and application thereof

Abstract

The application provides a municipal sludge microbial conditioner, a preparation method and application thereof. The conditioner comprises A-type composite bacteria and B-type composite bacteria. The A-type composite bacteria comprises Bacillus subtilis, Thiobacillus thioxidans, Thiobacillus denitrificans, Sphaerotilus natans, nitrifying bacteria, nitrosating bacteria and photosynthetic bacteria; the B-type composite bacteria comprises Lactobacillus acidophilus, Bifidobacterium, Saccharomyces cerevisiae, Saccharomyces uvarum, Candida lipolytica and Candida utilis. The application of the municipal sludge microbial conditioner: special culture medium is configured, the activated bacteria are respectively compounded to obtain the A-type composite bacteria and the B-type composite bacteria, then two-stage expansion culture is carried out, and finally the high-activity microbial conditioner is prepared. The microbial conditioner provided by the application can be applied under normal temperature conditions, can simultaneously and significantly reduce the water content of municipal sludge and inhibit the release of foul-smelling gas, thereby solving the problems of high energy consumption, high cost and serious secondary pollution of traditional dewatering technology.

Description

Technical Field

[0001] This invention relates to sludge reduction and microbial treatment technology, and more particularly to a municipal sludge microbial conditioner, its preparation method and application. Background Technology

[0002] With increasing urbanization and the gradual improvement of sewage collection systems, my country's sewage generation and treatment volume continue to rise. As of the end of 2023, the total treatment capacity of urban sewage treatment plants nationwide reached 270 million cubic meters per day, and the amount of sludge produced exceeded 86.9 million tons per year (based on an 80% moisture content). It is projected that by 2025, my country's municipal sludge production will exceed 90 million tons per year, and industrial sludge production will exceed 50 million tons per year, indicating a huge market size. Sludge dewatering is an effective means of reducing sludge volume; reducing the moisture content of sludge to below 50% can reduce sludge volume by more than 65%. Therefore, sludge dewatering to achieve volume reduction is crucial for subsequent treatment.

[0003] The water content in sludge can be broadly classified into four categories: A) interstitial water; B) capillary bound water; C) surface adsorbed water; and D) internal water. The first is called "free water," and the latter three are called "bound water." Except for interstitial water, which can be physically filtered out by pressure, the other three types are strongly coated with negative electrons on their surfaces, making them difficult to precipitate by physical pressure filtration. Interstitial water between particles accounts for approximately 65-85% of the sludge's water content; capillary water (including water within the sludge particles) accounts for approximately 15-25%; and adsorbed water and internal water within the particles account for approximately 10%. Sludge dewatering targets the interstitial water between particles, such as… Figure 1 As shown.

[0004] The high water content in sludge is largely due to the surface properties of its particles and the structure of the sludge clumps, in addition to interstitial water. These charged ions and extracellular polymers have a strong water-holding capacity. These sludge particles form sludge clumps, creating numerous capillary channels. The water held on the surface of the sludge particles and in the capillary channels is bound water, which cannot be removed by simple mechanical methods.

[0005] Sludge drying and dewatering is the core of sludge reduction and a key technology for sludge resource utilization. It is also the current technical bottleneck restricting the reduction, harmlessness, and resource utilization of excess biological sludge. Due to factors such as high operating costs, serious secondary pollution, and high safety risks associated with sludge drying, the development of my country's excess sludge treatment industry has been constrained for many years. In the past, the use of sanitary landfill, aerobic fermentation, anaerobic fermentation, land application, and thermal drying + incineration processes has resulted in serious economic waste and a relatively heavy environmental burden. Illegal discharge, stockpiling, and disposal of sludge have caused serious environmental pollution and huge economic losses, resulting in numerous adverse social impacts.

[0006] my country's sludge drying and dewatering technology has made significant progress over the years. Currently, my country mainly adopts a combined technology route of "chemical conditioning + physical pressing + thermal drying" to achieve sludge drying and dewatering. Commonly used equipment includes high / low temperature belt dryers, paddle dryers, thin-layer dryers, disc dryers, high-pressure plate and frame dryers, and high-pressure belt filter presses. These sludge drying and dewatering systems have been applied, but there are still many problems to be solved, such as high operating energy consumption, excessive use of chemical agents, and limited dewatering capacity. Summary of the Invention

[0007] The purpose of this invention is to address the problems of high energy consumption, high secondary pollution, and high treatment costs caused by the reliance on heat treatment and chemical conditioning in traditional municipal sludge dewatering technology. This invention proposes a municipal sludge microbial conditioner that can be applied at room temperature and can simultaneously and significantly reduce the moisture content of municipal sludge and inhibit the release of malodorous gases, thereby solving the problems of high energy consumption, high cost, and serious secondary pollution associated with traditional dewatering technology.

[0008] It should be noted that, in this invention, unless otherwise specified, the specific meaning of "comprising" in relation to composition and description includes both open-ended meanings such as "comprising," "including," etc., and closed-ended meanings such as "composed of," "consisting of," etc., and similar meanings.

[0009] To achieve the above objectives, the technical solution adopted by the present invention is: a municipal sludge microbial conditioner, comprising type A compound bacteria and type B compound bacteria;

[0010] The type A compound microbial strain contains the following components:

[0011] Bacillus subtilis (1.8-2.2) × 10 8 CFU / mL;

[0012] Thiobacillus thiooxidans (Tt) (0.3-0.7) × 10 8 CFU / mL;

[0013] Thiobacillus denitrificans (0.3-0.7) × 10 8 CFU / mL;

[0014] Planktonic Sphaerotilus natans (0.4-0.8) × 10 8 CFU / mL;

[0015] Nitrifying bacteria (0.4-0.8) × 10 8 CFU / mL;

[0016] Nitrifying bacteria (0.4-0.8) × 10 8 CFU / mL;

[0017] Photosynthetic bacteria (0.8-1.2) × 10 8 CFU / mL;

[0018] The type B complex microbial strain contains the following components:

[0019] Lactobacillus acidophilus (1.8-2.2) × 10 8 CFU / mL;

[0020] Bifidobacterium (1.8-2.2) × 10 8 CFU / mL;

[0021] Beer yeast (1.8-2.2) × 10 8 CFU / mL;

[0022] Ascomycetes (1.2-1.6) × 10 8 CFU / mL;

[0023] Candidalipolytica (0.8-1.2) × 10 8 CFU / mL;

[0024] Candida utilis (0.4-0.8) × 10 8 CFU / mL;

[0025] The remainder is culture medium.

[0026] The CFU / mL (Colony Forming Units per milliliter) mentioned in this invention refers to the number of viable bacteria per milliliter of sample that can form visible colonies under specific culture conditions, and is determined by plate counting method.

[0027] Furthermore, the mass ratio of type A compound bacteria and type B compound bacteria in the municipal sludge microbial conditioner is 1-2:1-2, preferably 1:1.

[0028] Another objective of this invention is to disclose the application of a municipal sludge microbial conditioner in the field of municipal sludge treatment. The type A and type B microbial conditioners, obtained by culturing type A and type B compound microbial strains, can improve the physical pressure filtration and dewatering effect of sludge and inhibit the release of malodorous gases when used in municipal sludge treatment.

[0029] Furthermore, the steps for using the municipal sludge microbial conditioner in municipal sludge treatment are as follows:

[0030] S1. Preparation of type A and type B microbial conditioners;

[0031] S1-1. Prepare the culture medium solution: Prepare the type A compound bacteria culture medium solution and the type B compound bacteria culture medium solution respectively;

[0032] S1-2. Preparation of compound strains: Activated Bacillus subtilis, Thiobacillus thiooxidans, Thiobacillus denitrificationis, planktonic scabra, nitrifying bacteria, nitrite-oxidizing bacteria, and photosynthetic bacteria are compounded in a certain proportion to obtain type A compound strain; activated Lactobacillus acidophilus, Bifidobacterium, Saccharomyces cerevisiae, Saccharomyces cerevisiae, Candida lipolyticus, and Candida utilis are compounded in a certain proportion to obtain type B compound strain;

[0033] S1-3. Preparation of original bacterial culture: The type A and type B compound bacterial cultures prepared in S1-2 are added to the corresponding type A and type B culture media prepared in S1-1 at a ratio of 1-2 wt%, respectively, and cultured in a fully enclosed reactor to obtain type A original bacterial culture and type B original bacterial culture, respectively.

[0034] S1-4. Prepare secondary culture media, and prepare type A compound bacterial culture media and type B compound bacterial culture media respectively, with the same composition as the culture media described in S1-1;

[0035] S1-5. Add the type A and type B original bacterial solutions prepared in S1-3 to the corresponding type A and type B compound bacterial culture media prepared in S1-4 at a ratio of 1-2 wt%, respectively. Incubate under the conditions of S1-3 to obtain type A and type B microbial conditioners, respectively. Secondary incubation promotes rapid bacterial proliferation, increases bacterial concentration, and forms a stable bacterial community structure.

[0036] S2. Add type A microbial conditioner and type B microbial conditioner to dechlorinated tap water at a mass ratio of 1:10-1:20 respectively, stir and mix well to obtain type A dilution and type B dilution;

[0037] S3. Add the type A diluent and type B diluent obtained in step S2 to the municipal sludge with a water content of 75-85% at a ratio of 0.5-1.5% and mix thoroughly.

[0038] S4. The mixed sludge is allowed to stand in the biochemical reaction tank for 16-24 hours, and the reacted sludge is then directly dewatered.

[0039] Further, the composition of the type A compound bacterial culture medium solution described in S1-1 is as follows: 70-90 parts brown sugar, 15-25 parts white sugar, 15-25 parts glucose, 14-16 parts peptone, 1.8-2.0 parts sodium chloride, 0.4-0.6 parts beef extract, 0.9-1.1 parts ammonium chloride, 0.4-0.6 parts dipotassium hydrogen phosphate, 0.18-0.22 parts magnesium chloride, 0.09-0.11 parts yeast extract, 0.9-1.1 parts sodium nitrite, 0.9-1.1 parts sodium carbonate, 0.4-0.6 parts magnesium sulfate, 0.3-0.5 parts ferrous sulfate, and 1000-1500 parts distilled water. All of the above are parts by weight.

[0040] Further, the composition of the B-type compound bacterial culture medium solution described in S1-1 is as follows: 70-90 parts brown sugar, 15-25 parts white sugar, 8-12 parts peptone, 8-12 parts beef extract, 8-12 parts yeast powder, 4-6 parts sodium acetate, 1.8-2.0 parts diammonium citrate, 75-80 parts Tween, 1.8-2.0 parts dipotassium hydrogen phosphate, 0.18-0.2 parts magnesium sulfate heptahydrate, 0.04-0.06 parts manganese sulfate heptahydrate, 0.9-1.1 parts magnesium chloride, 0.08-0.1 parts calcium chloride, 0.4-0.6 parts magnesium sulfate, 0.4-0.6 parts potassium sulfate, 0.9-1.1 parts ammonium sulfate, 0.9-1.1 parts sodium chloride, 0.01-0.11 parts red yeast rice powder, and 1000-1500 parts distilled water. All of the above are parts by weight.

[0041] Furthermore, the conditions for culturing type A compound bacteria in S1-3 are: temperature 30-32℃, oxygenation and maintaining dissolved oxygen concentration of 1.5-2.0 mg / L, continuous stirring, and culturing for 3-5 days.

[0042] Furthermore, the conditions for culturing the type B compound strain in S1-3 are: temperature 34-36℃, no oxygen, continuous stirring, and culturing for 3-5 days.

[0043] Furthermore, the material of the contact part of the fully enclosed reactor described in S1-3 and S1-5 is 304L grade stainless steel, the stirring speed inside the reactor is 28-32 r / min, and the reaction temperature is maintained by automatic water circulation heating through a jacket.

[0044] Furthermore, the air introduced during the cultivation of type A compound bacteria undergoes dust removal, sterilization, and purification treatment.

[0045] Furthermore, the total number of effective active bacteria in the type A and type B microbial conditioners respectively reaches 15 × 10⁻⁶. 8To ensure the concentration of effective ingredients in municipal sludge microbial conditioner is above CFU / mL, thereby improving product performance.

[0046] Furthermore, after S3 and before S4, water is added to the municipal sludge to adjust its moisture content to 88-92%, making it fluid, so as to increase the mass transfer efficiency and effect of the microbial conditioner, thereby improving the microbial conditioning efficiency and shortening the biochemical reaction time.

[0047] Furthermore, the mixing time in S3 is 60-120 minutes.

[0048] Furthermore, the dehydration described in S4 is mechanical pressure filtration dehydration, which employs a vertical pressing system.

[0049] Furthermore, when the type A and type B microbial conditioners are used in combination, the moisture content of municipal sludge after mechanical pressure filtration and dewatering is reduced to 20-30%.

[0050] Furthermore, the liquid-contact parts of the mixing and storage facilities for type A and type B diluents are made of 304L stainless steel, and the reactor is equipped with a stirring device.

[0051] Furthermore, the type A diluent must be stored with micropores at the bottom of the container for aeration.

[0052] Furthermore, the municipal sludge microbial conditioner should not be used simultaneously with disinfectants, antibiotics, or irritating chemical agents to avoid rendering the conditioner ineffective.

[0053] The municipal sludge microbial conditioner, its preparation method, and its application of the present invention have the following advantages compared with the prior art:

[0054] 1) After combined treatment with the type A and type B microbial conditioners of this invention, the moisture content of the sludge can be reduced to below 40%. This greatly promotes the reduction of sludge volume and creates favorable conditions for subsequent disposal (such as incineration, landfill, and resource utilization).

[0055] 2) This invention reduces odor precursors at the source through the metabolic activity of specific functional bacterial groups (carbon-fixing, denitrifying, and desulfurizing bacteria in type A bacteria). Experiments have shown that, compared with the blank control, the combined addition of type A and type B microbial conditioners significantly reduces multiple odor indicators such as H2S, C8H8, C2H6S, CH3SH, and OU, with an average reduction rate of 55.0% to 81.87%, and the NH3 concentration is reduced to zero. This effectively solves the problem of secondary air pollution during sludge treatment.

[0056] 3) The municipal sludge microbial conditioner of this invention is used to treat municipal sludge at room temperature, without the need for an external heat source, thus avoiding the large amount of heat energy consumed by traditional thermal drying processes and significantly reducing operating energy consumption and costs. By replacing or reducing the dosage of chemical conditioners (such as PAM) through microbial action, the cost of chemicals is further saved, and secondary pollution from chemical agents is reduced.

[0057] 4) Experiments have shown that the combined use of type A and type B microbial conditioners is more effective than using either one alone. Under the same dehydration rate, the combined use yields the best deodorization effect (e.g., the removal rates of H2S and C2H6S are higher than those of type A or type B alone), demonstrating that the specific bacterial combination designed in this invention produces a synergistic effect of "1+1>2".

[0058] 5) The preparation and application conditions of the bacterial agent of this invention (such as culture temperature, dissolved oxygen, reaction time, etc.) are all conventional and easily controllable biological process parameters, suitable for large-scale production and application. The application method is simple; after mixing the diluted bacterial solution with sludge and allowing it to stand for 16-24 hours, it can be put into existing mechanical dewatering equipment (such as plate and frame filter press) for dewatering, which is easy to carry out technical transformation and integration in existing sewage treatment plants.

[0059] In summary, the municipal sludge microbial conditioner of this invention can effectively improve the dewatering performance of municipal sludge under normal temperature conditions, and simultaneously and significantly inhibit the release of malodorous gases, thereby solving the pain points of high energy consumption, serious secondary pollution and high cost of traditional technical routes. It has good application prospects and large-scale promotion potential in the field of municipal sludge treatment. Attached Figure Description

[0060] Figure 1 This is a schematic diagram showing the water content of the sludge. Detailed Implementation

[0061] The present invention will be further described below with reference to embodiments. The description of the technical features described below is based on representative embodiments and specific examples of the present invention, but the present invention is not limited to these embodiments and specific examples. It should be noted that:

[0062] Unless otherwise stated, all units used in this specification are international standard units, and all numerical values ​​and ranges appearing in this invention should be understood to include systematic errors that are unavoidable in industrial production.

[0063] In this specification, the range of values ​​referred to as "value A to value B" refers to the range including the endpoint values ​​A and B.

[0064] In this specification, the numerical range indicated by "above" or "below" refers to the numerical range that includes the stated number.

[0065] In this specification, the word "may" has two meanings: to perform a certain process and not to perform a certain process.

[0066] In this specification, the terms "optional" or "optional" are used to indicate the use or omission of certain substances, components, procedures, application conditions, etc.

[0067] In this instruction manual, when "room temperature" or "room temperature" is used, the temperature can be 15-25℃.

[0068] Unless otherwise specified, all reagents or instruments used in this instruction manual are commercially available products.

[0069] The example uses the following pre-dewatering process to produce excess sludge from a municipal wastewater treatment plant. The excess biochemical sludge from the municipal wastewater treatment plant is temporarily stored in a sludge thickening tank. The sludge in the thickening tank has a moisture content of approximately 97-99%. The sludge is then pumped to the flocculation reaction tank at the front end of a belt sludge dewatering system. Anionic polyacrylamide flocculant (PAM) is added to the flocculation reaction tank, and after flocculation, the sludge enters the belt sludge dewatering system for pre-dewatering. After pre-dewatering, the sludge moisture content is reduced to 80-85%.

[0070] Example 1

[0071] This embodiment discloses a municipal sludge microbial conditioner, including type A compound bacterial strains and type B compound bacterial strains;

[0072] The type A compound microbial strain contains the following components:

[0073] Bacillus subtilis 2.0 × 10 8 CFU / mL;

[0074] Thiobacillus thiooxidans 0.5 × 10 8 CFU / mL;

[0075] Thiobacillus denitrification 0.7 × 10 8 CFU / mL;

[0076] Planktonic *Sphaerophyte* 0.6 × 10⁶ 8 CFU / mL;

[0077] Nitrifying bacteria 0.6 × 10 8 CFU / mL;

[0078] Nitrifying bacteria 0.6 × 10 8 CFU / mL;

[0079] Photosynthetic bacteria 1.0 × 10 8 CFU / mL;

[0080] The type B complex microbial strain contains the following components:

[0081] Lactobacillus acidophilus 2.0 × 10 8 CFU / mL;

[0082] Bifidobacterium 2.0 × 10 8 CFU / mL;

[0083] Brewer's yeast 2.0 × 10 8 CFU / mL;

[0084] Grape juice yeast 1.4×10 8 CFU / mL;

[0085] Candida lipolyticis 1.0×10 8 CFU / mL;

[0086] Candida utilis 0.6 × 10 8 CFU / mL.

[0087] The steps for using the municipal sludge microbial conditioner in municipal sludge treatment are as follows:

[0088] S1. Preparation of type A and type B microbial conditioners;

[0089] S1-1. Prepare culture medium solutions: Prepare type A compound bacterial culture medium solutions and type B compound bacterial culture medium solutions respectively;

[0090] Type A compound bacterial culture medium solution: 80 parts brown sugar, 20 parts white sugar, 20 parts glucose, 15 parts peptone, 2 parts sodium chloride, 0.5 parts beef extract, 1 part ammonium chloride, 0.5 parts dipotassium hydrogen phosphate, 0.2 parts magnesium chloride, 0.1 parts yeast extract, 1 part sodium nitrite, 1 part anhydrous sodium carbonate, 0.5 parts magnesium sulfate, 0.4 parts ferrous sulfate, and 1000 parts distilled water. Mix thoroughly to form the Type A compound bacterial culture medium solution.

[0091] Type B compound bacterial culture medium: 80 parts brown sugar, 20 parts white sugar, 10 parts peptone, 10 parts beef extract, 10 parts yeast powder, 5 parts sodium acetate, 2 parts diammonium citrate, 80 parts Tween, 2 parts dipotassium hydrogen phosphate, 0.2 parts magnesium sulfate heptahydrate, 0.05 parts manganese sulfate heptahydrate, 1 part magnesium chloride, 0.1 part calcium chloride, 0.5 parts magnesium sulfate, 0.5 parts potassium sulfate, 1 part ammonium sulfate, 1 part sodium chloride, 0.01 parts red yeast rice powder, and 1000 parts distilled water.

[0092] S1-2. Preparation of compound strains: Activated Bacillus subtilis, Thiobacillus thiooxidans, Thiobacillus denitrificationis, planktonic scabra, nitrifying bacteria, nitrite-oxidizing bacteria, and photosynthetic bacteria are compounded in a certain proportion to obtain type A compound strain; activated Lactobacillus acidophilus, Bifidobacterium, Saccharomyces cerevisiae, Saccharomyces cerevisiae, Candida lipolyticus, and Candida utilis are compounded in a certain proportion to obtain type B compound strain;

[0093] S1-3. Preparation of original bacterial cultures: The type A and type B compound bacterial strains prepared in S1-2 were respectively added to the corresponding type A and type B culture media prepared in S1-1 and cultured in a completely closed reactor. The type A culture temperature was 30℃, and the type B culture temperature was 35℃. During the type A culture, oxygen was purged and the DO concentration was maintained at 2.0 mg / L with continuous stirring. During the type B culture, no oxygen was purged and continuous stirring was maintained. After 5 days of culture, the primary original bacterial cultures of type A and type B were obtained and stored in a sealed container for use as inoculation for secondary culture. Primary original bacterial cultures of type A and type B were obtained respectively.

[0094] S1-4. Prepare a secondary culture medium with the same composition as the culture medium solution described in S1-1;

[0095] S1-5. Add the type A and type B original bacterial solutions prepared in S1-3 to the corresponding type A and type B secondary culture solutions prepared in S1-4 at a ratio of 1%, respectively, and culture them according to the conditions in S1-3 to obtain type A microbial conditioner and type B microbial conditioner, respectively.

[0096] The tests showed that the total number of effective active bacteria in both the type A and type B microbial conditioners reached 15 × 10⁻⁶. 8 CFU / mL or higher.

[0097] The steps for using the municipal sludge microbial conditioner in municipal sludge treatment are as follows:

[0098] Take 1 cubic meter of pre-dewatered sludge into a fully enclosed mixing reaction tank. The mixing reaction tank is equipped with a frame-type agitator with a stirring speed of 50 revolutions per minute. Turn on the agitator and simultaneously add 10% diluted solutions of two types of microbial agents, A and B, at a ratio of 1%. Stir for 60 minutes to ensure that the materials are fully mixed. Turn off the agitator and let it stand for 16 hours. After 16 hours, pass the reacted sludge into the vertical sludge pressing system. The maximum output pressure of the pressing system is controlled at 7.0 MPa. The average moisture content of the dewatered sludge reaches 30.59%.

[0099] During the implementation process, the average values ​​of six exhaust gas indicators (H2S, NH3, C8H8, C2H6S, CH3SH, and OU) in the top space of the mixing reaction tank were 0.09 mg / m³. 3 0.00 mg / m 3 5.97 mg / m3 3.61 mg / m 3 1.16 mg / m 3 21.00 mg / m 3 .

[0100] Comparative Example 1

[0101] Take 1 cubic meter of pre-dewatered sludge into a fully enclosed mixing reaction tank. The mixing reaction tank is equipped with a frame-type agitator with a stirring speed of 50 revolutions per minute. Turn on the agitator and add 10% diluted solution of the same type A microbial conditioner as in Example 1 at a ratio of 1%. Stir for 60 minutes to ensure that the materials are fully mixed. Turn off the agitator and let it stand for 16 hours. After 16 hours, the reacted sludge is fed into a vertical sludge pressing system. The maximum output pressure of the pressing system is controlled at 7.0 MPa. The average moisture content of the dewatered sludge reaches 35.48%.

[0102] During the implementation process, the average values ​​of six exhaust gas indicators (H2S, NH3, C8H8, C2H6S, CH3SH, and OU) in the top space of the mixing reaction tank were 0.08 mg / m³. 3 0.00 mg / m 3 11.47 mg / m 3 7.75 mg / m 3 1.86 mg / m 3 39.00 mg / m 3 .

[0103] Comparative Example 2

[0104] Take 1 cubic meter of pre-dewatered sludge into a fully enclosed mixing reaction tank. The mixing reaction tank is equipped with a frame-type agitator with a stirring speed of 50 revolutions per minute. Turn on the agitator and add 10% diluted solution of the same type B microbial conditioner as in Example 1 at a ratio of 1%. Stir for 60 minutes to ensure that the materials are fully mixed. Turn off the agitator and let it stand for 16 hours. After 16 hours, the reacted sludge is fed into a vertical sludge pressing system. The maximum output pressure of the pressing system is controlled at 7.0 MPa. The average moisture content of the dewatered sludge reaches 34.65%.

[0105] During the implementation process, the average values ​​of six exhaust gas indicators (H2S, NH3, C8H8, C2H6S, CH3SH, and OU) in the top space of the mixing reaction tank were 0.21 mg / m³. 3 0.00 mg / m 3 7.82 mg / m 3 6.75 mg / m 3 2.98 mg / m 3 45.00 mg / m 3 .

[0106] Comparative Example 3

[0107] Take 1 cubic meter of pre-dewatered sludge into a fully enclosed mixing reaction tank. The mixing reaction tank is equipped with a frame-type agitator with a stirring speed of 50 revolutions per minute. Turn on the agitator and add dechlorinated tap water at a ratio of 1%. Stir for 60 minutes to ensure that the materials are fully mixed. Turn off the agitator and let it stand for 16 hours. After 16 hours, the reacted sludge is fed into a vertical sludge pressing system. The maximum output pressure of the pressing system is controlled at 7.0 MPa. The average moisture content of the dewatered sludge reaches 48.91%.

[0108] During the implementation process, the average values ​​of six exhaust gas indicators (H2S, NH3, C8H8, C2H6S, CH3SH, and OU) in the top space of the mixing reaction tank were 0.20 mg / m³. 3 0.00 mg / m 3 13.86 mg / m 3 9.04 mg / m 3 6.40 mg / m 3 59.00 mg / m 3 .

[0109] Through data comparison, under the premise that other control factors remain unchanged, the effect of adding type A / B bacterial agent is the best. The dehydration rate increased by 18.32% compared with no bacterial agent added. The average reduction values ​​of six waste gas indicators, H2S, NH3, C8H8, C2H6S, CH3SH and OU, reached 55.0%, 0%, 56.9%, 60.06%, 81.87% and 64.40%, respectively.

[0110] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A municipal sludge microbial conditioner, characterized in that, Including type A and type B compound strains; The type A compound microbial strain contains the following components: Bacillus subtilis (1.8-2.2) × 10 8 CFU / mL; Thiobacillus thiooxidans (0.3-0.7) × 10 8 CFU / mL; Thiobacillus denitrification (0.3-0.7) × 10 8 CFU / mL; Planktonic *Sphaerophytes* (0.4-0.8) × 10⁻⁶ 8 CFU / mL; Nitrifying bacteria (0.4-0.8) × 10 8 CFU / mL; Nitrifying bacteria (0.4-0.8) × 10 8 CFU / mL; Photosynthetic bacteria (0.8-1.2) × 10 8 CFU / mL; The type B complex microbial strain contains the following components: Lactobacillus acidophilus (1.8-2.2) × 10 8 CFU / mL; Bifidobacteria (1.8-2.2) × 10 8 CFU / mL; Brewer's yeast (1.8-2.2) × 10 8 CFU / mL; Grape juice yeast (1.2-1.6) × 10 8 CFU / mL; Candida lipolyticis (0.8-1.2) × 10 8 CFU / mL; Candida utilis (0.4-0.8) × 10 8 CFU / mL.

2. The municipal sludge microbial conditioner according to claim 1, characterized in that, The mass ratio of the type A compound strain to the type B compound strain is 1-2:1-2.

3. The application of the municipal sludge microbial conditioner as described in claim 1 or 2 in the field of municipal sludge treatment.

4. The application according to claim 3, characterized in that, The steps for using the municipal sludge microbial conditioner in municipal sludge treatment are as follows: S1. Preparation of type A and type B microbial conditioners; S1-1. Prepare the culture medium solution: Prepare the type A compound bacteria culture medium solution and the type B compound bacteria culture medium solution respectively; S1-2. Preparation of compound strains: Activated Bacillus subtilis, Thiobacillus thiooxidans, Thiobacillus denitrificationis, planktonic scabra, nitrifying bacteria, nitrite-oxidizing bacteria, and photosynthetic bacteria are compounded in a certain proportion to obtain type A compound strain; activated Lactobacillus acidophilus, Bifidobacterium, Saccharomyces cerevisiae, Saccharomyces cerevisiae, Candida lipolyticus, and Candida utilis are compounded in a certain proportion to obtain type B compound strain; S1-3. Preparation of original bacterial culture: The type A and type B compound bacterial cultures prepared in S1-2 are added to the corresponding type A and type B culture media prepared in S1-1 at a ratio of 1-2 wt%, respectively, and cultured in a fully enclosed reactor to obtain type A original bacterial culture and type B original bacterial culture, respectively. S1-4. Prepare secondary culture media, and prepare type A compound bacterial culture media and type B compound bacterial culture media respectively, with the same composition as the culture media described in S1-1; S1-5. Add the type A and type B original bacterial solutions prepared in S1-3 to the corresponding type A and type B compound bacterial culture media prepared in S1-4 at a ratio of 1-2 wt%, respectively, and culture them under the conditions of S1-3 to obtain type A microbial conditioner and type B microbial conditioner, respectively. S2. Add type A microbial conditioner and type B microbial conditioner to dechlorinated tap water at a ratio of 1:10-1:20 respectively, stir and mix well to obtain type A dilution and type B dilution; S3. Add the type A diluent and type B diluent obtained in step S2 to the municipal sludge with a water content of 75-85% at a ratio of 0.5-1.5% and mix thoroughly. S4. The mixed sludge is allowed to stand in the biochemical reaction tank for 16-24 hours, and the reacted sludge is then directly dewatered.

5. The application according to claim 4, characterized in that, The composition of the type A compound bacterial culture medium is as follows: 70-90 parts brown sugar, 15-25 parts white sugar, 15-25 parts glucose, 14-16 parts peptone, 1.8-2.0 parts sodium chloride, 0.4-0.6 parts beef extract, 0.9-1.1 parts ammonium chloride, 0.4-0.6 parts dipotassium hydrogen phosphate, 0.18-0.22 parts magnesium chloride, 0.09-0.11 parts yeast extract, 0.9-1.1 parts sodium nitrite, 0.9-1.1 parts sodium carbonate, 0.4-0.6 parts magnesium sulfate, 0.3-0.5 parts ferrous sulfate, and 1000-1500 parts distilled water. All of the above are parts by weight. And / or, the composition of the type B compound bacterial culture medium is as follows: 70-90 parts brown sugar, 15-25 parts white sugar, 8-12 parts peptone, 8-12 parts beef extract, 8-12 parts yeast powder, 4-6 parts sodium acetate, 1.8-2.0 parts diammonium citrate, 75-80 parts Tween, 1.8-2.0 parts dipotassium hydrogen phosphate, 0.18-0.2 parts magnesium sulfate heptahydrate, 0.04-0.06 parts manganese sulfate heptahydrate, 0.9-1.1 parts magnesium chloride, 0.08-0.1 parts calcium chloride, 0.4-0.6 parts magnesium sulfate, 0.4-0.6 parts potassium sulfate, 0.9-1.1 parts ammonium sulfate, 0.9-1.1 parts sodium chloride, 0.01-0.11 parts red yeast rice powder, and 1000-1500 parts distilled water. All of the above are parts by weight.

6. The application according to claim 4, characterized in that, The conditions for culturing type A compound bacteria in S1-3 are: temperature 30-32℃, oxygenation and maintaining dissolved oxygen concentration of 1.5-2.0 mg / L, continuous stirring, and culturing for 3-5 days; And / or, the conditions for culturing type B compound bacteria are: temperature 34-36℃, no oxygen, continuous stirring, and culturing for 3-5 days.

7. The application according to claim 4, characterized in that, The material of the parts of the fully enclosed reactors described in S1-3 and S1-5 that come into contact with the material is 304L grade stainless steel. And / or, the stirring speed in the reactor is 28-32 r / min; And / or, maintain the reaction temperature by automatic water circulation heating via a jacket.

8. The application according to claim 4, characterized in that, The air introduced during the cultivation of type A compound bacteria is treated with dust removal, sterilization and purification.

9. The application according to claim 4, characterized in that, The mixing time in S3 is 60-120 minutes.

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