Pseudomonas soli, microbial inoculant and application thereof

Abstract

The application discloses Pseudomonas songnenensis, a microbial inoculum and application thereof, and relates to the technical field of environmental microorganisms and solid waste resource technology. The Pseudomonas songnenensis strain JP01 is preserved in the China General Microbiological Culture Collection Center, and has a preservation number of CGMCC No. 37102. The strain has salt and alkali tolerance characteristics, and can degrade coal gangue and promote the release of effective phosphorus, available potassium and effective silicon nutrients. After inoculation for 4 days, under normal conditions, the effective phosphorus content of the strain JP01 treatment group is 758.65% higher than that of the CK group; under salt and alkali conditions, the effective phosphorus content of the strain JP01 treatment group is 4.70 times that of the bacillus megaterium treatment group. The strain has good long-term colonization ability in a coal gangue-salt and alkali soil mixed substrate, and the viable cell count can still be maintained at a high level of 2.72x10 7 CFU / g after 90 days of culture. The application is suitable for coal gangue resource utilization and salt and alkali soil improvement, and has good phosphorus element activation ability and functional stability in a salt and alkali environment.

Description

Technical Field

[0001] This invention relates to the fields of environmental microbiology technology and solid waste resource utilization technology, specifically to a Songnen Plain Pseudomonas aeruginosa, a microbial agent, and its application. Background Technology

[0002] Coal gangue is a large-scale industrial solid waste generated during coal mining and washing. Large-scale stockpiling not only occupies land but also causes environmental pollution. On the other hand, coal gangue is rich in various nutrients such as silicon, potassium, phosphorus, and calcium, but these elements exist mostly in stable, insoluble forms such as siliceous minerals, aluminosilicate minerals, and phosphate minerals, making them difficult for plants to directly absorb and utilize, thus limiting its resource application value in agriculture.

[0003] Meanwhile, soil salinization is a global land degradation problem that seriously threatens agricultural production and ecological security. The high pH and high salinity environment of saline-alkali soils severely inhibits soil microbial activity, leading to soil infertility. Utilizing microbial technology to activate nutrients in coal gangue and use them to improve saline-alkali soil can achieve the goal of "treating waste with waste." However, ordinary microorganisms struggle to survive and colonize under saline-alkali stress, and their ability to degrade stable minerals in coal gangue is also very limited. Therefore, screening strains that possess both salt and alkali tolerance and efficient coal gangue degradation capabilities is crucial for advancing this technology.

[0004] Existing microbial agents used for coal gangue degradation or saline-alkali soil improvement have the following shortcomings: most strains lack salt and alkali tolerance, and their growth is severely inhibited in high-salt and alkaline environments, making it difficult for them to effectively colonize and function in saline-alkali soils; their activation efficiency for insoluble nutrients in coal gangue is low, especially their ability to release key elements such as phosphorus, potassium, and silicon; and their long-term survival and colonization stability in complex coal gangue-saline-alkali soil mixed systems are poor. Summary of the Invention

[0005] To address the technical problems existing in the prior art, embodiments of the present invention provide a *Pseudomonas aeruginosa* species from Songnen Plain, a microbial inoculant, and its application. The technical solution is as follows:

[0006] A strain of *Pseudomonas songnenensis*, JP01, is deposited at the China General Microbiological Culture Collection Center (CGMCC) under accession number CGMCC No. 37102.

[0007] A microbial agent comprising: the *Pseudomonas songnenensis* strain JP01, and a vector.

[0008] The application of the aforementioned *Pseudomonas songnenensis* strain JP01 or the aforementioned microbial agent in the preparation of formulations for degrading coal gangue.

[0009] The application of the aforementioned *Pseudomonas songnenensis* strain JP01 or the aforementioned microbial agent in the preparation of formulations that activate phosphorus in coal gangue.

[0010] The application of the aforementioned *Pseudomonas songnenensis* strain JP01 or the aforementioned microbial agent in the preparation of saline-alkali soil conditioner.

[0011] The application of the aforementioned Pseudomonas songnenensis strain JP01 or the aforementioned microbial agent in the method of improving saline-alkali soil.

[0012] A method for degrading coal gangue, the method comprising the following steps: applying the aforementioned Pseudomonas songnenensis strain JP01 or the aforementioned microbial agent to the coal gangue.

[0013] A method for activating phosphorus in coal gangue, the method comprising the following steps: applying the aforementioned Pseudomonas songnenensis strain JP01 or the aforementioned microbial agent to the coal gangue.

[0014] A method for improving saline-alkali soil, the method comprising the following steps: applying the aforementioned *Pseudomonas songnenensis* strain JP01 or the aforementioned microbial agent to the saline-alkali soil.

[0015] The beneficial effects of the technical solutions provided in the embodiments of the present invention include at least the following:

[0016] This invention discloses a salt-alkali tolerant bacterium for degrading coal gangue and its applications. The strain, *Pseudomonas songnenensis*, was deposited on December 18, 2025, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 37102. This strain exhibits salt-alkali tolerance, with an optimal growth pH of 8.0. It can grow normally in LB medium at pH 9.0 and a NaCl concentration of 1.0%, and can degrade coal gangue, promoting the release of available phosphorus, available potassium, and available silicon. Four days after inoculation, under normal conditions, the available phosphorus content in the coal gangue treated with strain JP01 reached 89.3 mg / kg, an increase of 758.65% compared to the control group (CK). Under salt-alkali conditions, the available phosphorus content in the coal gangue treated with strain JP01 reached 87.5 mg / kg, 4.70 times that of the *Bacillus megaterium* treatment group. Meanwhile, the contents of available potassium and available silicon in the JP01 treatment group were also higher than those in the CK group and the Bacillus megaterium treatment group. The strain exhibited good long-term colonization ability in a coal gangue-saline-alkali soil mixed substrate, maintaining a viable count of 2.72 × 10⁻⁶ after 90 days of cultivation. 7 The invention exhibits a relatively high level of CFU / g. It is applicable to the resource utilization of coal gangue and the improvement of saline-alkali soil.

[0017] 1. Highly efficient degradation of coal gangue: Strain JP01 can promote the release of key nutrients in coal gangue, especially phosphorus, thereby improving the resource utilization value of coal gangue.

[0018] 2. Strong environmental adaptability: Its strong salt and alkali resistance enables it to survive and function in saline and alkali environments.

[0019] 3. Compared with common phosphorus-solubilizing bacteria such as Bacillus megaterium, strain JP01 can promote the release of available phosphorus in coal gangue under both normal and saline-alkali conditions, and can maintain good phosphorus activation ability, especially under saline-alkali conditions; at the same time, it also has a certain promoting effect on the release of available potassium and available silicon, showing better environmental adaptability and functional stability.

[0020] 4. Stable colonization ability: It can survive stably for a long time in a mixed substrate of coal gangue and saline-alkali soil, ensuring its lasting effect in field application.

[0021] 5. Green and environmentally friendly: It realizes the resource utilization of coal-based solid waste, provides a green and sustainable biological solution for saline-alkali soil treatment, and reduces environmental risks and treatment costs.

[0022] 6. Wide range of applications: This strain can be used as a core strain to develop compound microbial agents, bio-organic fertilizers, and special soil conditioners for saline-alkali soils.

[0023] [Preservation Information] Pseudomonas songnenensis strain JP01, whose original strain number was YZJ-JLMGS-1, was deposited on December 18, 2025, at the China General Microbiological Culture Collection Center (CGMCC, address: No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing), with accession number CGMCCNo. 37102. Attached Figure Description

[0024] To more clearly illustrate the technical solutions in the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0025] Figure 1 These are colony morphology and microscopic images of strain JP01 provided in Example 1 of this invention; wherein... Figure 1 Image (a) shows the colony morphology of strain JP01. Figure 1 (b) is a microscopic morphological diagram of strain JP01;

[0026] Figure 2 This is a phylogenetic tree diagram of strain JP01 constructed based on the 16S rRNA gene sequence provided in Example 1 of the present invention;

[0027] Figure 3 This is a growth curve of strain JP01 provided in Example 2 of the present invention under different salinities;

[0028] Figure 4 This is a growth curve of strain JP01 provided in Example 2 of the present invention at different pH values;

[0029] Figure 5 This is a graph showing the results of the long-term colonization ability test of strain JP01 provided in Example 3 of the present invention in a mixed matrix of coal gangue and saline-alkali soil.

[0030] Figure 6 This is a comparison diagram of the nutrient release effects of different strains on coal gangue under normal and saline-alkali conditions, provided in Example 4 of the present invention. Figure 6 Figure (a) shows the comparison results under normal conditions. Figure 6 (b) shows the comparison results under saline-alkali conditions;

[0031] Figure 7 These are the XRD patterns of strain JP01 before and after treatment of coal gangue provided in Example 4 of this invention; wherein, Figure 7In the middle (a), the XRD pattern of the control group (CK) coal gangue is shown. Figure 7 (b) shows the XRD pattern of the JP01 treatment group. Detailed Implementation

[0032] The technical solution of the present invention will now be described with reference to the accompanying drawings.

[0033] In embodiments of the present invention, words such as "exemplarily," "for example," etc., are used to indicate that something is an example, illustration, or description. Any embodiment or design described as "exemplary" in the present invention should not be construed as being more preferred or advantageous than other embodiments or designs. Specifically, the use of the word "exemplary" is intended to present the concept in a concrete manner. Furthermore, in embodiments of the present invention, the meaning expressed by "and / or" can be both, or either one.

[0034] This invention relates to a functional strain with salt and alkali tolerance, capable of degrading coal gangue and promoting its nutrient release, and its applications. The invention aims to provide a functional strain with salt and alkali tolerance and the ability to degrade coal gangue and activate nutrients, thereby addressing the problems of poor adaptability of existing strains in saline-alkali environments and low activation efficiency of various nutrients in coal gangue. This provides a core strain resource for the development of efficient saline-alkali soil biological amendments and coal gangue resource utilization technologies.

[0035] To achieve the above objectives, the present invention provides the following technical solution:

[0036] This invention provides a salt-tolerant coal gangue-degrading bacterium, namely *Pseudomonas songnenensis*, referred to as strain JP01 in this invention. This strain was deposited on December 18, 2025, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 37102.

[0037] The strain JP01 has the following characteristics:

[0038] • Gram-negative, cells are rod-shaped, and colonies on TSA medium are round, slightly yellow, raised, and smooth.

[0039] • It has salt and alkali tolerance: it can grow in the pH range of 6.0-9.0, with the optimal growth pH being 8.0; it can also grow normally under NaCl concentration of 1.0%.

[0040] • It can grow in inorganic salt culture media with coal gangue as the main screening substrate;

[0041] • It can degrade coal gangue and promote the release of nutrients within it: Four days after inoculation with this strain, under normal conditions, the available phosphorus content in coal gangue reached 89.3 mg / kg, which was 758.65% higher than that of the CK group; under saline-alkali conditions, the available phosphorus content in coal gangue reached 87.5 mg / kg, which was 4.70 times that of the Bacillus megaterium treatment group; at the same time, the contents of available potassium and available silicon also increased to varying degrees.

[0042] • It has good colonization ability: it can still maintain a high number of viable bacteria after being cultured in a sterilized coal gangue-saline soil mixed substrate for 90 days;

[0043] • Metabolic characteristics: It can produce acidic metabolites and may promote the release of phosphorus from phosphorus-containing minerals such as apatite by lowering the pH value of the coal gangue microenvironment.

[0044] The present invention provides a microbial inoculant comprising the strain JP01 and an agriculturally acceptable carrier.

[0045] This invention provides the application of the strain JP01 or the microbial agent in the degradation of coal gangue.

[0046] This invention provides the application of the strain JP01 or the microbial agent in the activation of phosphorus in coal gangue.

[0047] This invention provides the application of the strain JP01 or the microbial agent in the preparation of saline-alkali soil conditioner.

[0048] The present invention provides a method for improving saline-alkali soil, which includes applying the strain JP01 or the microbial agent together with coal gangue to the saline-alkali soil.

[0049] To make the technical problems, technical solutions and advantages of the present invention clearer, a detailed description will be given below in conjunction with the accompanying drawings and specific embodiments.

[0050] Unless otherwise specified, the experimental methods described in the following embodiments are conventional experimental methods well known to those skilled in the art, and are performed according to the techniques or conditions described in the literature in this field or according to the product instructions. Where specific conditions are not specified in the experimental methods, they are generally operated under conventional conditions.

[0051] Unless otherwise specified, all materials and reagents described in the following examples are commercially available.

[0052] Example 1: Screening and Identification of Strains JP01

[0053] Using coal gangue from the Gaotouyao Coal Mine in Dalad Banner, Ordos City, Inner Mongolia, as the bacterial source, and after acclimatization to salt and alkali (pH 9, 1% NaCl) for 7 days, selective screening was conducted using a selective salt-alkali culture medium with coal gangue as the main screening substrate. The acclimatization method is as follows: Coal gangue from the Gaotouyao Coal Mine in Dalad Banner, Ordos City, Inner Mongolia, was pulverized by a ball mill and passed through a 200-mesh sieve to obtain coal gangue powder. 10 g of coal gangue powder was weighed and added to 100 mL of sterile saline-alkali water (pH 9.0, 1% NaCl mass fraction), and placed in a constant temperature shaking incubator at 30℃ and 180 rpm for acclimatization and culture for 7 days. During this period, sterile saline-alkali water was added every 2 days to maintain the initial volume. After acclimatization, the obtained bacterial solution was used as the bacterial source for subsequent screening.

[0054] The formula for a selective salt-alkali culture medium using coal gangue as the main screening substrate is as follows: Weigh 4.20 g of Na2HPO4·H2O, 1.75 g of KH2PO4, 0.50 g of NH4Cl, 0.25 g of MgSO4·7H2O, and 0.01 g of CaCl2, dissolve them in 500 mL of distilled water, add 50 g of coal gangue powder (200 mesh), 8 g of agar, and 5 g of NaCl, adjust the pH to 9.0 with 1 mol / L NaOH solution, add distilled water to a total volume of 500 mL, mix well, and autoclave at 121℃ for 30 min to obtain the selective salt-alkali solid culture medium for coal gangue.

[0055] The targeted screening method is as follows: Take 1 mL of the acclimatized bacterial culture and add it to 9 mL of sterile physiological saline. Perform serial dilutions of 10-fold to obtain 10... -2 10 -3 10 -4 10 -5 10 -6 Bacterial suspensions at various dilutions. Take 100 μL of each dilution and spread it evenly on a coal gangue selective saline-alkali solid medium plate. Set up three replicates for each dilution and incubate upside down at 30℃ for 3-5 days. Observe colony growth. Select colonies with significantly different morphologies and the best survival status, streak them on the same medium plate for purification, repeating three times until a pure culture is obtained, named strain JP01.

[0056] The strain was subjected to morphological observation and Gram staining, and then sent to the China Agricultural Microbiology Preservation Center for 16S rRNA gene sequence analysis.

[0057] Experimental results:

[0058] Experimental results are as follows Figure 1 and Figure 2 As shown.

[0059] Figure 1 These are colony morphology and microscopic images of strain JP01. Figure 1 Image (a) shows the colony morphology of strain JP01 after incubation at 30°C for 24 h on TSA medium. Figure 1 As can be seen in (a), the colonies are round, slightly yellow, smooth, with neat edges and a raised center. Figure 1 Image (b) shows cell morphology under an optical microscope (×1000x) after Gram staining. Figure 1 As can be seen in (b), the cells are short rod-shaped with blunt ends, arranged singly or in pairs, and Gram-negative (red).

[0060] The 16S rRNA gene sequence of strain JP01 is as follows:

[0061]

[0062] Figure 2 This is a phylogenetic tree diagram of strain JP01 constructed based on the 16S rRNA gene sequence, provided in an embodiment of the present invention. From... Figure 2 As can be seen from the sequence alignment, the 16S rRNA gene sequence of the strain of the present invention has a similarity of up to 99.85% with Pseudomonassongnenensis NEAU-ST5-5T (Accession: RFFN01000014).

[0063] Based on its colony morphology (round, slightly yellow, raised) and physiological characteristics, it was identified as *Pseudomonas songnenensis*, namely strain JP01 of this invention, and was deposited at CGMCC on December 18, 2025, with accession number CGMCC No. 37102.

[0064] Example 2: Determination of salt and alkali tolerance and growth characteristics of strain JP01

[0065] The activated strain JP01 was inoculated into LB liquid medium containing 1.0% NaCl at different initial pH (6.0, 7.0, 8.0, 9.0) and into LB liquid medium with different NaCl concentrations (0.5%, 1.0%, 1.5%, 2.0%) (pH 9.0). The cultures were incubated at 30℃ and 180 rpm for 70 h with shaking, and the OD was measured at different time points. 600 Values ​​are used to plot growth curves.

[0066] Experimental results:

[0067] Experimental results are as follows Figure 3 and Figure 4 As shown.

[0068] Figure 3 This is a growth curve of strain JP01 provided in an embodiment of the present invention under different salinities. From... Figure 3 It can be seen that strain JP01 can still grow within the NaCl concentration range of 0.5%-2.0%, and reaches OD200 after 35 h under 0.5% NaCl conditions. 600 Peak value 1.45.

[0069] Figure 4 This is a growth curve of strain JP01 provided in an embodiment of the present invention at different pH values. From... Figure 4 It can be seen that strain JP01 can grow in the pH range of 6.0-9.0, with the optimal growth pH being 8.0, reaching OD at 46 h. 600 Peak value 1.65.

[0070] The above results indicate that strain JP01 has a wide pH adaptability and strong salt tolerance.

[0071] Example 3: Determination of the long-term colonization ability of strain JP01 in coal gangue-saline-alkali soil matrix

[0072] The activated strain JP01 was inoculated into LB liquid medium and cultured at 30°C with shaking at 180 rpm until the logarithmic growth phase. The bacterial cells were collected by centrifugation, washed and resuspended with sterile physiological saline, and the OD was adjusted. 600 A value of 1.0 (corresponding to a viable count of approximately 1 × 10⁻⁶) 8 (CFU / mL) to obtain a bacterial suspension for colonization experiments. Inoculate the suspension into a sterile mixed substrate of coal gangue and saline-alkali soil (1:1, w / w) and incubate at 30°C for 90 days. Periodically sample and dilute the suspension, then spread it onto a selective saline-alkali solid medium containing coal gangue to count the number of viable bacteria. The formulation of the selective saline-alkali medium with coal gangue as the primary screening substrate is the same as in Example 1.

[0073] Experimental results:

[0074] Experimental results are as follows Figure 5 As shown.

[0075] Figure 5 This is a graph showing the results of the long-term colonization ability test of strain JP01 provided in Example 3 of this invention in a mixed matrix of coal gangue and saline-alkali soil. From... Figure 5 It can be seen that after 90 days of cultivation in a mixed substrate of coal gangue and saline-alkali soil, the viable count of strain JP01 can still be maintained at 2.72 × 10⁻⁶. 7 The high level of CFU / g indicates that it possesses excellent long-term colonization ability.

[0076] Example 4: Comparison of the nutrient release effect of strain JP01 on coal gangue

[0077] Strains JP01 and control strains were inoculated into a reaction system based on coal gangue, and their nutrient release capabilities under different environmental conditions were compared and analyzed. The *Bacillus megaterium* strain is a common phosphate-solubilizing bacterium and was used to represent existing microorganisms for nutrient activation in coal gangue. The construction method of this reaction system is as follows:

[0078] Weigh 10 g of coal gangue powder that has passed through a 200-mesh sieve, dispense it into 100 mL wide-mouth bottles, autoclave at 121℃ for 30 min, and cool before use.

[0079] The strain JP01 was prepared into OD according to the method in Example 2. 600 The bacterial suspension was prepared at a concentration of 1.0. Simultaneously, *Bacillus megaterium*, a common phosphate-solubilizing bacterium, was also selected and its suspension was prepared using the same method (OD). 600=1.0). Take 30 mL of the above bacterial suspension and add it to a wide-mouth bottle containing sterile coal gangue powder. Mix thoroughly with a sterile L-rod. Add an equal volume of sterile physiological saline to the blank control group. Set up treatment groups as CK group (added with sterile physiological saline), JP01 group (inoculated with strain JP01), and control group (inoculated with Bacillus megaterium). Set up two culture conditions at the same time: normal conditions (pH 7.0, NaCl mass fraction 0.5%) and saline-alkali conditions (pH 9.0, NaCl mass fraction 1.0%). Incubate the samples of each treatment group in a constant temperature incubator at 30℃ for 4 days. Each treatment is repeated 3 times.

[0080] After cultivation, the samples were dried at 40℃, ground, and passed through a 100-mesh sieve for nutrient content determination. The determination method is as follows:

[0081] Available phosphorus: The 0.5 mol / L NaHCO3 extraction-molybdenum antimony colorimetric method was used, specifically referring to the method in "Soil Agrochemical Analysis" (Bao Shidan, 3rd edition). The absorbance was measured at a wavelength of 700 nm using a UV-Vis spectrophotometer, and the content was calculated based on the standard curve.

[0082] Available potassium: The potassium content of the extract was determined by leaching with 1.0 mol / L NH4OAc and flame photometry.

[0083] Available silicon: The absorbance was measured at 700 nm using a UV-Vis spectrophotometer by citric acid-sodium bisulfite buffer extraction-silica molybdenum blue colorimetric method, and the available silicon content was calculated.

[0084] To further analyze the effect of strain JP01 on the mineral structure of coal gangue, XRD analysis was performed on samples before and after treatment. The specific methods are as follows:

[0085] The dried and ground samples were analyzed using an X-ray diffractometer (DX2700B, Dandong Haoyuan). Test conditions: Cu target, Kα radiation (λ=0.15406 nm), tube voltage 40 kV, tube current 40 mA, scanning range 5°–75° (2θ), step size 0.02°, scanning speed 4° / min. The obtained diffraction patterns were analyzed for phase retrieval and peak intensity using Jade software.

[0086] Experimental results:

[0087] Experimental results are as follows Figure 6As shown in the figure. Under normal conditions, namely pH 7.0 and NaCl mass fraction 0.5%, the available phosphorus content in the coal gangue of the JP01 treatment group reached 89.3 mg / kg. Compared with the CK group, the available phosphorus content of the JP01 treatment group increased by 758.65%; compared with the Bacillus megaterium treatment group, the available phosphorus content of the JP01 treatment group was 2.78 times that of the Bacillus megaterium treatment group. Simultaneously, the available potassium and available silicon contents in the JP01 treatment group were also higher than those in the CK group and the Bacillus megaterium treatment group. These results indicate that under normal conditions, strain JP01 can effectively promote the release of phosphorus from coal gangue and also has a certain promoting effect on the release of nutrients such as potassium and silicon. Its comprehensive nutrient activation effect is superior to that of common phosphorus-solubilizing bacteria such as Bacillus megaterium. Figure 6 As shown in (a).

[0088] Under saline-alkali conditions (pH 9.0, NaCl mass fraction 1.0%), the available phosphorus content in the coal gangue of the JP01 treatment group reached 87.5 mg / kg. Compared with the CK group, the available phosphorus content of the JP01 treatment group increased by 792.86%; compared with the Bacillus megaterium treatment group, the available phosphorus content of the JP01 treatment group was 4.70 times that of the Bacillus megaterium treatment group. Under saline-alkali conditions, the available potassium and available silicon contents of the JP01 treatment group were still higher than those of the CK group and the Bacillus megaterium treatment group. Further comparison of the results under normal and saline-alkali conditions showed that the phosphorus solubilization capacity of Bacillus megaterium decreased significantly under saline-alkali conditions, while JP01 could still maintain an available phosphorus release level close to that under normal conditions under saline-alkali conditions. The above results indicate that strain JP01 is less affected by saline-alkali environments, retains a strong ability to activate phosphorus in coal gangue under saline-alkali stress, and can also promote the release of nutrients such as potassium and silicon. Therefore, it is suitable for the resource utilization of coal gangue and the improvement of nutrients in saline-alkali soil under saline-alkali environments. Figure 6 As shown in (b).

[0089] In summary, strain JP01 can not only promote the release of available phosphorus in coal gangue under normal conditions, but also maintain a high phosphorus activation capacity under saline-alkali conditions. At the same time, it also has a certain promoting effect on the release of available potassium and available silicon in coal gangue, indicating that strain JP01 is suitable for the resource utilization of coal gangue and the improvement of nutrients in saline-alkali soil under saline-alkali conditions.

[0090] Figure 7 These are the XRD patterns of strain JP01 before and after treatment of coal gangue, provided in Example 4 of this invention. Figure 7 (a) shows the XRD pattern of the control group (CK) coal gangue. Figure 7 (b) shows the XRD pattern of the JP01 strain treatment group. Figure 7As shown, 1 represents quartz, 2 represents kaolinite, 3 represents apatite minerals, 4 represents illite, 5 represents potassium feldspar, and 6 represents pyrite. Compared with the control group, the weak diffraction peak intensity of apatite minerals near 2θ≈31.8° in the JP01 treatment group decreased, indicating that the structure of phosphorus-containing minerals was affected to some extent, which corresponds to the efficient phosphorus solubilization function of strain JP01. The intensity of the main peak of quartz (2θ≈26.7°) also decreased, and the peak shape broadened, indicating that the silicon-oxygen framework was loosened. At the same time, some characteristic diffraction peaks of aluminosilicate minerals such as kaolinite, illite, and potassium feldspar also showed a weakening trend, indicating that the crystal structure of aluminosilicate minerals in coal gangue was affected to some extent, corresponding to the release of nutrients such as potassium and silicon. XRD analysis results show that strain JP01 can affect the mineral crystal structure in coal gangue through biodegradation, thereby promoting the release of nutrients.

[0091] The above examples demonstrate that the strain JP01 provided by the present invention has good salt and alkali tolerance, coal gangue degradation ability, nutrient activation ability and long-term colonization stability, and is suitable for coal gangue resource utilization and saline-alkali soil improvement.

[0092] The above description is merely a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in the present invention should be included within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.

Claims

1. A strain of *Pseudomonas songnenensis* JP01, characterized in that, The *Pseudomonas s. Songnen Plain* strain is deposited at the China General Microbiological Culture Collection Center (CGMCC), accession number CGMCC No. 37102.

2. A microbial inoculant, characterized in that, The microbial agent comprises: the Pseudomonas songnenensis strain JP01 as described in claim 1, and a vector.

3. The application of the *Pseudomonas songnenensis* strain JP01 according to claim 1 or the microbial agent according to claim 2 in the preparation of a formulation for degrading coal gangue.

4. The application of the *Pseudomonas songnenensis* strain JP01 according to claim 1 or the microbial agent according to claim 2 in the preparation of a formulation for activating phosphorus in coal gangue.

5. The application of the *Pseudomonas songnenensis* strain JP01 according to claim 1 or the microbial agent according to claim 2 in the preparation of saline-alkali soil conditioner.

6. The application of the *Pseudomonas songnenensis* strain JP01 of claim 1 or the microbial agent of claim 2 in the method of improving saline-alkali soil.

7. A method for degrading coal gangue, characterized in that, The method includes the following steps: applying the Pseudomonas songnenensis strain JP01 as described in claim 1 or the microbial agent as described in claim 2 to coal gangue.

8. A method for activating phosphorus in coal gangue, characterized in that, The method includes the following steps: applying the Pseudomonas songnenensis strain JP01 as described in claim 1 or the microbial agent as described in claim 2 to coal gangue.

9. A method for improving saline-alkali soil, characterized in that, The method includes the following steps: applying the *Pseudomonas songnenensis* strain JP01 according to claim 1 or the microbial agent according to claim 2 to saline-alkali soil.

Images