Priestia aryabhattai YJ and a bacterial agent and application thereof

Abstract

The present application relates to a strain Priestia aryabhattai YJ and its bacterial agent and application, and belongs to the technical field of microorganisms. The application belongs to the technical field of microorganisms. Priestia aryabhattai ) YJ is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 36146, the preservation date is October 11, 2025, and the preservation agency address is No. 3, Beichen West Road, Chaoyang District, Beijing. The present application also provides a kind of Priestia aryabhattai Bacterial agent of YJ and its application in the production of indole acetic acid and in the promotion of rye grass and wheat in saline-alkali soil. The present application provides a kind of Priestia aryabhattai YJ is a kind of excellent rhizosphere growth-promoting bacteria, which has the excellent characteristics of salt tolerance, phosphorus solubilization, potassium solubilization, high yield of indole acetic acid, etc.

Description

Technical Field

[0001] This invention relates to the field of microbial technology, specifically to a strain Priestia aryabhattai YJ and its inoculants and applications. Background Technology

[0002] Measures to improve saline-alkali land can be broadly categorized into four aspects: natural restoration, physical methods, chemical methods, and bioremediation. Natural restoration primarily involves restricting human activities, such as implementing fencing, allowing vegetation to gradually recover through its own ecological cycle. However, this method suffers from long recovery cycles and unpredictable results. Chemical methods involve applying soil conditioners such as gypsum and humic acid to adjust soil pH and ionic composition, thereby reducing soil salinity. While its effects are short-lived, it is relatively expensive, and improper application can cause secondary pollution. Physical methods mainly rely on engineering techniques such as leaching, installing underground drainage systems, and returning straw to the field to regulate soil and improve saline-alkali soil. Its advantage lies in its rapid effectiveness, quickly improving local soil physicochemical properties, but it suffers from drawbacks such as large engineering workload, high costs, and potential damage to the original soil structure. Ecological restoration methods utilize the synergistic effects of salt-tolerant plants and soil microorganisms to improve saline-alkali soil. This method offers numerous advantages, including low cost, ease of implementation and promotion, safety and no pollution, and continuous and stable desalination effects, effectively curbing the spread of salinity. Compared with other improvement measures, ecological restoration has shown its advantages in both economic and ecological benefits and is considered a long-term effective and sustainable measure for improving saline-alkali land.

[0003] Plant rhizosphere growth-promoting bacteria (PGPR) are a class of beneficial bacteria that colonize the rhizosphere of plants and can directly or indirectly promote plant growth. Their growth-promoting mechanisms include: secreting plant hormones such as indoleacetic acid (IAA) and cytokinins to stimulate plant cell division and root growth; or converting organic and inorganic phosphorus in the soil into forms that plants can absorb and utilize through acidolysis and mineralization; and indirectly improving the soil environment and increasing the bioavailability of soil nutrients through nitrogen fixation, phosphorus solubilization, potassium solubilization, and iron production, thereby promoting plant growth and enhancing their adaptability to environmental stress. However, currently available strains with multiple growth-promoting functions generally have weak tolerance to saline-alkali stress and are difficult to colonize in saline-alkali environments to exert their growth-promoting effects. Therefore, screening for growth-promoting bacteria with salt stress tolerance and multiple growth-promoting functions is of great significance for the improvement of saline-alkali soils.

[0004] Argentinella pristi ( Priestia aryabhattai *Primulina auris* is a multifunctional agricultural bacterium that can produce indoleacetic acid (IAA) to promote plant growth. However, existing *Primulina auris* strains generally exhibit weak tolerance to saline-alkali stress, and their ability to produce IAA is also relatively weak. How to address these issues to promote plant growth in saline-alkali soils is an urgent problem to be solved. Summary of the Invention

[0005] To address the problems of existing technologies, such as the generally weak tolerance of *Priscilla auriculata* to salt and alkali stress and its low ability to produce indoleacetic acid, this invention provides a strain... Priestia aryabhattai YJ and its inoculants and applications are used to solve the above problems.

[0006] In a first aspect, the present invention provides a plant Priestia aryabhattai YJ, the aforementioned Priestia aryabhattai YJ is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 36146, on October 11, 2025. The address of the depository is No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing.

[0007] Furthermore, the aforementioned Priestia aryabhattai The 16S rDNA sequence of YJ is shown in SEQ ID NO.3.

[0008] Secondly, the present invention provides a product containing Priestia aryabhattai YJ's bacterial agent.

[0009] Furthermore, in the bacterial agent Priestia aryabhattai YJ viable count ≥ 1.5 × 10⁻⁶ 8 CFU / mL.

[0010] Furthermore, the bacterial agent is a liquid bacterial agent.

[0011] Furthermore, the preparation method of the liquid bacterial agent is as follows: Pick Priestia aryabhattai A single colony of *YJ* was inoculated into a test tube containing LB liquid medium and cultured at 37°C for 18 hours on a shaker to obtain a seed culture. The seed culture was then inoculated at a 5% inoculation rate into 400 ml of LB liquid medium and cultured at 30°C and 150 rpm for 16 hours. After centrifugation at 8000 rpm for 10 minutes, the supernatant was discarded, and the bacterial pellet was diluted with sterile distilled water to an OD value of [missing value]. 600 A value of 1.0 is obtained, containing Priestia aryabhattai YJ's bacterial agent.

[0012] Furthermore, the LB liquid culture medium comprises the following components: 10g tryptone, 5g yeast extract, 10g NaCl, 1000mL distilled water, pH=7.2.

[0013] Thirdly, the present invention provides Priestia aryabhattai YJ was used in the production of indoleacetic acid, with ≥90μg / mL of indoleacetic acid produced by 5-day fermentation.

[0014] Fourthly, the present invention provides Priestia aryabhattaiYJ was used to promote the growth of ryegrass in saline-alkali land, where the salt content was 50-100 mol / L.

[0015] Fifthly, the present invention provides Priestia aryabhattai Application of YJ in promoting wheat growth in saline-alkali land.

[0016] The beneficial effects of this invention are as follows: The present invention provides Priestia aryabhattai YJ is an excellent rhizosphere growth-promoting bacterium with superior characteristics such as salt tolerance, phosphorus solubilization, potassium solubilization, and high production of indoleacetic acid (IAA) in a short period of time. It can tolerate a salt concentration of 11%, has a Ca3(PO4)2 solubility of 363 μg / mL, and produces up to 93.13 μg / mL of IAA. In addition, strain YJ also exhibits excellent nitrogen fixation and siderophore production characteristics. The results of the examples demonstrate that the present invention… Priestia aryabhattai The YJ strain has a good growth-promoting effect on ryegrass and wheat, and has a promising application prospect. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, for those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 This is in Embodiment 1 of the present invention Priestia aryabhattai Colony morphology diagram of YJ.

[0019] Figure 2 This is in Embodiment 1 of the present invention Priestia aryabhattai Chart showing the iron-carrier capacity of YJ.

[0020] Figure 3 This is a comparison diagram of the effects of wheat growth promotion experiment in Example 5 of the present invention. Detailed Implementation

[0021] To enable those skilled in the art to better understand the technical solutions of this invention, the technical solutions of the embodiments of this invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this invention, and not all embodiments. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without creative effort should fall within the scope of protection of this invention.

[0022] The various culture medium components used in the embodiments of this invention are as follows: LB solid medium: 10g tryptone, 5g yeast extract, 10g NaCl, 1000mL distilled water, pH=7.2, 18g agar, sterilized at 121℃ for 20min.

[0023] LB liquid medium: 10g tryptone, 5g yeast extract, 10g NaCl, 1000mL distilled water, pH=7.2, sterilized at 121℃ for 20min.

[0024] PVK liquid medium: glucose 10g; Ca3(PO4)2 5g; (NH4)2SO4 0.5g; NaCl 0.2g; KCl 0.2g; MgSO4·7H2O 0.1g; yeast extract 0.5g; MnSO4·H2O 0.002g; FeSO4·7H2O 0.002g; distilled water 1000mL; sterilize at 115℃ for 30min.

[0025] CAS detection medium: Chromium azurite S 60.5 mg, hexadecyltrimethylammonium bromide (HDTMA) 72.9 mg, ferric chloride hexahydrate 2.645 mg, sodium dihydrogen phosphate dihydrate 295.25 mg, disodium hydrogen phosphate dodecahydrate 1213.5 mg, ammonium chloride 125 mg, potassium dihydrogen phosphate 37.5 mg, sodium chloride 62.5 mg, agar 9000 mg, distilled water 1000 mL, pH=6.8±0.1, autoclaved at 121℃ for 20 min.

[0026] Ashube nitrogen-fixing medium: K2HPO4 0.2g; NaCl 0.2g; MgSO4 0.2g; CaCO3 5g; K2SO4 0.1g; mannitol 10g; agar 15g; distilled water 1000mL; pH=7.0; 25℃; sterilize at 121℃ for 20min.

[0027] Example 1 Priestia aryabhattai Isolation, screening and identification of YJ 1. Sampling: On September 18, 2022, healthy wild salt mustard (Haloxylon ammodendron) was selected from the Yellow River Delta region of Binzhou City, Shandong Province (118°1'E, 37°57'N). T he llung ie ll a sa l s ug i nea) Rhizosphere soil was collected from the plants. All samples were collected randomly, and the sample processing methods were consistent. When collecting rhizosphere soil samples, a 5m × 5m square quadrat was drawn in an area with healthy plants at each sampling point. Three rhizosphere soil sampling points were randomly selected within each quadrat. Loose weeds and debris near the sampling area were removed. The soil was then scooped up from the plant roots to a depth of approximately 15cm. Large clumps of soil were then gently shaken off from the plant roots. A sterile brush was used to gently sweep the rhizosphere soil adhering to the plant root surface into a sterile bag. The soil sample was stored with ice packs and immediately transported to the laboratory for screening and isolation of salt-tolerant rhizosphere bacteria.

[0028] 2. Separation: Add 10g of root soil to 90mL of sterile physiological saline, place on a shaker at 30℃ and 180 rpm for 30min, then shake well and allow to settle until soil sedimentation, collect the supernatant. Perform a serial dilution of the supernatant using a serial dilution method, taking 10g of each solution. -3 10 -4 10 -5 Three serially diluted solutions were spread onto LB agar plates and incubated at 30°C. Under aseptic conditions, different single colonies were picked and streaked onto new plates for purification until all colonies on the plates were purified. Single colonies with different morphological characteristics were numbered and stored, and their growth-promoting effects were measured. The strain with the best effect was obtained for further research and named YJ.

[0029] 3. Identification (1) Identification of colony morphology Strain strain YJ was inoculated onto LB solid medium and incubated at 30°C for 2-3 days. YJ colonies were observed to be milky white, moist, opaque, and with neat edges. (See attached image). Figure 1 As shown. Strain YJ is a Gram-positive bacterium that produces spores and is mesophytic.

[0030] (2) Identification of physiological and biochemical characteristics Strain YJ can utilize glucose, sucrose, and mannitol; it cannot utilize xylose and L-arabinose; it is negative for VP test and positive for OF test; it is negative for hydrogen sulfide test; it is positive for gelatin hydrolysis and starch hydrolysis; it is negative for arginine dihydrolase; it can utilize citrate; it is negative for tartrate; it is positive for casein hydrolysis, catalase, oxidase, and nitrate reduction; its growth temperature is 4℃~55℃, and its NaCl tolerance range is 0~11%.

[0031] (3) Identification of 16S rDNA gene sequence Single colonies of strain YJ were inoculated into LB liquid medium and cultured at 32℃ and 180 r / min for 24 h. The bacterial cells were collected by centrifugation, and bacterial DNA was extracted using a bacterial extraction kit. Universal primers 27F (5-AGAGTTTGATCCTGGCTCAG-3, SEQ ID NO.1) and 1492R (5-TACGGCTACCTTGTTACGACTT-3, SEQ ID NO.2) were selected for 16S rDNA extraction. The PCR reaction mixture consisted of: 2 μL genomic DNA, 2 μL dNTPs (10 mmol / L), 5 μL 10×Buffer, 2 μL universal primer 27F, 2 μL universal primer 1492R, 2 μL Taq polymerase (2.5 U / μL), and 35 μL ddH2O. PCR amplification conditions for the 16S rDNA gene: 94℃ for 5 min; 94℃ for 1 min; 58℃ for 30 s; 70℃ for 90 s, 35 cycles; 72℃ for 10 min. The amplified products were analyzed by 1% agarose gel electrophoresis and then sent to the Wuhan branch of Sangon Biotech (Shanghai) Co., Ltd. for sequencing. The 16S rDNA gene sequence is 1487 bp in length. The 16S rDNA sequence of strain YJ is shown in SEQ ID NO.3. The sequence was compared with that of *Primatex auriculata* in the NCBI database. Priestia aryabhattai The strain YJ showed the highest similarity to *Priscilla auriculata*. Therefore, strain YJ was identified as *Priscilla auriculata*. Priestia aryabhattai ). Priestia aryabhattai YJ is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 36146, on October 11, 2025. The address of the depository is No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing.

[0032] Example 2 1. Determination of ability to produce indoleacetic acid (IAA) Weigh 10.0 mg of IAA standard into a beaker, dissolve it in anhydrous ethanol, and dilute to 10 mL. Mix well and store in a brown bottle protected from light. Add 0, 10, 20, 30, 40, and 50 μL of the standard solution to six test tubes, respectively. Dilute each test tube to 1 mL with sterile water and mix well. Perform triplicate for each test tube. Add 1 mL of Salkowski colorimetric solution to each test tube, mix thoroughly, and allow to stand in the dark for 30 min. Measure the OD using a UV spectrophotometer. 530 A standard curve was constructed based on the concentration of 3-indoleacetic acid standard solution and its corresponding absorbance value. The activated strain was inoculated into a solution containing L-tryptophan (500 mg·L⁻¹). -1 The culture was incubated in LB broth at 30°C and 150 rpm for 6 days. During the incubation period, 2 mL of bacterial suspension was collected daily, centrifuged at 4000 rpm for 10 min, and 1 mL of the supernatant was collected. An equal volume of Salkowski colorimetric solution was added and mixed well. The mixture was allowed to stand in the dark for 30 min, and the OD was measured using a UV spectrophotometer. 530 The IAA content produced by the bacteria was calculated using a standard curve. See Table 1 for detailed results.

[0033] Table 1- Priestia aryabhattai YJ production of indoleacetic acid (IAA) level

[0034] As can be seen from Table 1, Priestia aryabhattai YJ can produce high levels of indoleacetic acid (IAA), with the highest IAA content of 93.13 μg / mL after 5 days of culture.

[0035] 2. Phosphorus solubility test Pipette 0, 2, 4, 6, 8, and 10 mL of 5 mg / L K₂HPO₄ standard solution into 50 mL volumetric flasks. Add 1-2 drops of methyl orange indicator, and adjust the solution to a pale yellow color by micro-addition of NaOH solution (1 mM) and HCl solution (1 mM). Then, accurately add 5 mL of molybdenum antimony colorimetric solution, shake thoroughly, and dilute to 50 mL with deionized water. Incubate at room temperature for 30 min, and measure the OD of each standard solution using a UV spectrophotometer. 700 A standard curve is constructed based on the concentration of the standard solution and its corresponding absorbance value. The activated solution... Priestia aryabhattai YJ was inoculated into PVK liquid medium. The control group was inoculated with an equal volume of sterile water and incubated at 30℃ and 150 rpm for 7 days. During the incubation period, 2 mL of bacterial suspension was taken daily and centrifuged at 11000 rpm for 5 min. 50 μL of the supernatant was added to a volumetric flask, along with 1-2 drops of dinitrophenol indicator and 1-2 drops of methyl orange indicator. The solution was adjusted to a slightly yellow color by micro-addition of NaOH solution (1 mM) and HCl solution (1 mM). Then, 5 mL of molybdenum antimony anti-colorimetric solution was accurately added, and the mixture was thoroughly mixed. The volume was brought to 50 mL with deionized water and incubated at room temperature for 30 min. The OD was measured and recorded using a UV spectrophotometer. 700 The phosphorus content was calculated based on the standard curve. See Table 2 for detailed test results.

[0036] Table 2- Priestia aryabhattai YJ phosphorus dissolution test results

[0037] 3. Determination of iron production capacity Prepare CAS detection medium, sterilize it, pour it into plates, and after the plates solidify, add the activated medium. Priestia aryabhattai YJ used sterile toothpicks to spot-inoculate the strain onto a plate and incubated it upside down at 30°C for 48 hours. Observation and recording were performed. If the strain produced a distinct orange-yellow discoloration zone on the CAS plate, it indicated that the strain had the ability to secrete siderophores; the larger the discoloration zone and the deeper the color, the stronger the strain's siderophore-producing ability. Results ( Figure 2 The results showed that strain YJ has the ability to produce siderophores.

[0038] 4. Nitrogen fixation capacity determination Priestia aryabhattai YJ was inoculated onto Assumption nitrogen-fixing medium using the streak plating method and incubated at 30°C for one week, observing for colony formation. If colonies formed, single colonies were picked out and inoculated onto Assumption nitrogen-fixing medium again until the third generation of colonies could still grow on Assumption nitrogen-fixing medium, indicating that the strain had nitrogen-fixing ability. The experimental results showed that... Priestia aryabhattai YJ can grow normally on a solid plate, indicating that it has nitrogen-fixing ability.

[0039] Example 3 Priestia aryabhattai Preparation of YJ bacterial agent Pick Priestia aryabhattai A single colony of *YJ* was inoculated into a test tube containing LB liquid medium and cultured at 37°C for 18 hours on a shaker to obtain a seed culture. The seed culture was then inoculated at a 5% inoculation rate into 400 ml of LB liquid medium and cultured at 30°C and 150 rpm for 16 hours. After centrifugation at 8000 rpm for 10 minutes, the supernatant was discarded, and the bacterial pellet was diluted with sterile distilled water to an OD value of [missing value]. 600 A value of 1.0 is obtained, containing Priestia aryabhattai YJ's bacterial agent.

[0040] Example 4 Salt stress Priestia aryabhattai YJ's effect on promoting the growth of ryegrass The ryegrass seed variety was Blue Label "Gentleman", purchased from Shuyang Haociran Green Seedling Co., Ltd. Select plump seeds of uniform size and free from pests, rinse with 75% ethanol for 30 seconds, then wash with sterile water 4-5 times. Soak the cleaned seeds in 5% NaClO solution for 2 minutes, rinse with sterile water multiple times, and then sow evenly in 30cm×25cm×5cm seedling pots. Cultivate in a 25℃ constant temperature incubator, watering with sterile water until germination. Select seeds with uniform growth and good condition and transfer them to hydroponic pots containing 700ml Hoagland nutrient solution for cultivation. Sow 27 pretreated ryegrass seeds in each pot. Two salinity gradients were set up: 50mmol / L NaCl and 100mmol / L NaCl. The experimental treatments were: (1) Control (CK), with 50mL of sterile water added; (2) Inoculated with the seeds prepared in Example 3. Priestia aryabhattai YJ bacterial suspension 50mL, with 3 replicates per treatment. Hydroponic pots were placed in a constant-temperature incubator with a light intensity of 6000 Lx, a light cycle of 14h light / 10h darkness, and a temperature of 25℃ during the day / 22℃ during the night, and cultured continuously for 35 days. Water was replenished periodically during the experiment. After 35 days of culture, 9 ryegrass plants were randomly selected. The plants were removed along with their roots, rinsed thoroughly with clean water, and dried with filter paper. The fresh and dry weights, plant height, root length, and other relevant indicators of the above-ground and underground parts were measured and recorded. Plant height was measured directly from the top of the root to the top of the plant using a ruler. Root length, root surface area, and other root-related indicators were measured using a Wanshen LA-S root analyzer. The leaves and roots of the ryegrass plants were separated, and their fresh weight was measured using an electronic balance. They were then placed in an oven at 105℃ for 0.5h to blanch, followed by drying at 80℃ to constant weight, and the dry weights of the leaves and roots were measured. The results are shown in Table 3.

[0041] Table 3 - Inoculation under Salt Stress Conditions Priestia aryabhattai The effect of YJ on ryegrass growth

[0042] According to Table 3, the application... Priestia aryabhattai YJ can significantly promote the growth of ryegrass.

[0043] Example 5 Priestia aryabhattai The influence of YJ on wheat growth Soil selection: moderately salinized soils were selected from the Yellow River Delta region.

[0044] Experimental steps: The experiment was conducted in plastic basins (15cm×14cm×11cm), each containing 3kg of test soil that had passed through a 2mm sieve. The application rates of base fertilizers N, P2O5, and K2O were 0.20, 0.15, and 0.15 g / kg soil, respectively, and the fertilizers were analytical grade urea, calcium phosphate, and potassium chloride. The soil prepared in Example 3 was used... Priestia aryabhattai Add 300 mL of YJ bacterial solution to the soil, mix thoroughly, and then fill plastic basins with water to 60% of field capacity. Select plump wheat seeds (Jimai 22), disinfect with 5% NaClO for 5 min, rinse with sterile water and soak for 6 h, then wrap in damp gauze and let stand overnight before sowing. Sow 15 seeds per basin, with 10 basins per treatment. During the experiment, water was added by weight to maintain the soil moisture content at approximately 60% of field capacity. After 21 days of cultivation, observe the growth of wheat seedlings. See details below. Figure 3 .Depend on Figure 3 It was found that after applying the YJ strain described in this application, the wheat plant height, root length, plant fresh weight, and root fresh weight were all significantly higher than the control (P < 0.05). Plant height increased by 4.37 cm compared to the control, an increase of 60.05%; root length increased by 1.63 cm, an increase of 36.54%; plant fresh weight increased by 0.024 g, an increase of 26.86%; and root fresh weight increased by 0.012 g, an increase of 79.18%. These results indicate that strain YJ has a good promoting effect on the growth of wheat seedlings. Although the present invention has been described in detail with reference to the accompanying drawings and preferred embodiments, the present invention is not limited thereto. Various equivalent modifications or substitutions can be made to the embodiments of the present invention by those skilled in the art without departing from the spirit and essence of the invention, and such modifications or substitutions should all be within the scope of the present invention. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in the present invention should also be covered within the protection scope of the present invention.

Claims

1. A single plant Priestia aryabhattai YJ, characterized in that, The Priestia aryabhattai YJ is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 36146, on October 11, 2025. The address of the depository is No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing.

2. As described in claim 1 Priestia aryabhattai YJ, characterized in that, The Priestia aryabhattai 16S rDNA sequence of YJ is shown in SEQ ID NO.

3.

3. A device comprising the following as described in claim 1 Priestia aryabhattai YJ's bacterial agent.

4. The microbial agent as described in claim 3, characterized in that, The bacterial agent Priestia aryabhattai YJ viable count ≥ 1.5 × 10⁻⁶ 8 CFU / mL.

5. The microbial agent as described in claim 3, characterized in that, The bacterial agent is a liquid bacterial agent.

6. The microbial agent as described in claim 5, characterized in that, The preparation method of the liquid bacterial agent is as follows: Pick Priestia aryabhattai YJ single colonies were inoculated into test tubes containing LB liquid medium and cultured at 37°C for 18 hours on a shaker to obtain seed culture. The seed culture was then inoculated at a 5% inoculation rate into 400 ml of LB liquid medium and cultured at 30°C and 150 rpm for 16 hours. After centrifugation at 8000 rpm for 10 minutes, the supernatant was discarded, and the bacterial pellet was diluted with sterile distilled water to an OD value of 1. 600 A value of 1.0 is obtained, containing Priestia aryabhattai YJ's bacterial agent.

7. The microbial agent as described in claim 6, characterized in that, The LB liquid culture medium comprises the following components: 10g tryptone, 5g yeast extract, 10g NaCl, 1000mL distilled water, pH=7.

2.

8. A device as described in claim 1 Priestia aryabhattai The application of YJ in the production of indoleacetic acid is characterized by, Priestia aryabhattai YJ produces ≥90μg / mL of indoleacetic acid during five days of fermentation.

9. A device as described in claim 1 Priestia aryabhattai Application of YJ in promoting ryegrass growth in saline-alkali land.

10. A device as described in claim 1 Priestia aryabhattai Application of YJ in promoting wheat growth in saline-alkali land.

Images