Liquid gene chip for wheat low cadmium and comprehensive agronomic trait identification and breeding, kit and application thereof

Abstract

The application belongs to the technical field of plant molecular breeding, biochip and molecular detection, and particularly relates to a liquid gene chip for wheat low-cadmium and comprehensive agronomic trait identification and breeding, a kit thereof and application thereof.The gene chip comprises detection reagents for specifically detecting a SNP site set, and the SNP site set is selected from the following: a cadmium-related SNP site set, a homologous function gene cadmium-related SNP site set, other metal ion-related SNP site set, a SNP site set related to Nramp, HMA, ABC, ZIP, PCR, MTP, YSL and CAX gene families, and a whole genome background filled SNP site set.The application realizes systematic detection of wheat cadmium absorption, transport, accumulation and detoxification related genetic variation, realizes multi-metal coordinated monitoring and heavy metal accumulation panoramic scanning, can be used for comprehensive risk assessment of wheat heavy metal accumulation traits, improves complex variation detection capability, has excellent detection performance, is high in information acquisition efficiency, and is suitable for large-scale application.