A portable general-purpose optical detection device
Patent Information
- Application Number
- CN202610721159.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-05-25
- Publication Date
- 2026-08-21
AI Technical Summary
[0002]RPA(重组酶聚合酶扩增)与CRISPR-Cas12a技术的结合,已实现了高特异性、高灵敏度的核酸快检,但其读出环节仍多依赖大型设备(如荧光定量PCR仪、酶标仪等),受限于设备昂贵、操作复杂、能耗高及对实验室环境的严格依赖,难以在基层医疗机构、现场执法、家庭自测及资源匮乏地区推广
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Figure CN122612468A_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of optical inspection device technology, and specifically relates to a portable universal optical inspection device. Background Technology
[0002] The combination of RPA (recombinase polymerase amplification) and CRISPR-Cas12a technology has enabled rapid nucleic acid detection with high specificity and sensitivity. However, the readout process still largely relies on large-scale equipment (such as quantitative PCR instruments and ELISA readers). Limited by the high cost of equipment, complex operation, high energy consumption, and strict dependence on laboratory environments, it is difficult to promote its application in primary healthcare institutions, on-site law enforcement, home testing, and resource-scarce areas. Furthermore, in practical applications, samples often require multiple optical detections, typically involving multiple sample preparations and individual testing, which is time-consuming and labor-intensive. Those skilled in the art need a device that can hold samples and is easily detachable from various optical detection instruments, enabling rapid detection. Summary of the Invention
[0003] To address the aforementioned problems, this invention provides a portable universal optical detection device, comprising a detection chamber and an observation chamber. The detection chamber, from top to bottom, includes an operating area, a detection area, and a light source area. The operating area has an openable and closable top cover for placing and removing sample vials into the operating area and the detection area. Each side of the detection chamber has an openable and closable interface for connecting to other external optical detection devices to perform optical detection on the samples within the detection chamber. An observation chamber is located in front of the detection area, and a detachable filter is provided between the detection area and the observation chamber for observing the fluorescence effect of the samples.
[0004] The testing chamber is equipped with a row of fixed brackets and a row of movable racks. The movable racks include several moving parts for placing several sample bottles and can move the sample bottles individually to be tested by the light source of other optical testing devices.
[0005] A movable excitation source is provided in the light source area. The excitation source is located below the sample bottle and is used to illuminate the bottom of the sample bottle upwards for fluorescence detection.
[0006] The device of this invention can directly perform fluorescence detection, has a simple structure, is easy to carry, and can also reposition individual sample vials. When the entire device is connected to other optical detection instruments, other optical detections can be performed on the repositioned individual sample vials. Specifically, a row of several sample vials is placed on the bracket and moving frame. The excitation light source moves below the sample vials, which can individually illuminate the bottom of each sample vial, avoiding background fluorescence and scattered light induced by the excitation light illuminating the side walls of the sample vials. The lower middle part of the sample vials is located in the detection area, and the operator can horizontally observe the fluorescence effect of the sample vials in the detection area from the outside of the observation room. When other optical detections are required, the corresponding sample vial is moved between two docking ports using the moving component. The docking ports on both sides of the device can connect to other optical detection devices. The light source of the other optical detection device enters the detection area from one docking port, illuminates the sample vial, and then exits from the other docking port. The light is received and analyzed by the receiving component of the optical detection device to obtain the detection result.
[0007] Optionally, the three areas of the detection chamber are vertically connected, the three areas are of equal length, the operating area and the detection area are of equal width, the rear sides of the three areas are on the same vertical plane, and the front sides of the operating area and the detection area are on the same vertical plane, so that the operating area and the detection area are completely vertically aligned, making it easy for the sample bottle to pass through the operating area and the detection area.
[0008] The width of the light source area is greater than the width of the detection area. The front part of the light source area is located below the observation chamber. The excitation light source and its associated moving components are located at the rear of the light source area, while the control circuit components are located at the front of the light source area.
[0009] Further optionally, the moving component includes a base and a lead screw, the lead screw being parallel to the length direction of the light source area, and both ends of the lead screw extending through the two wide sides of the light source area, respectively, to facilitate the operator to rotate the lead screw;
[0010] The base has a laser light source at the top and a through hole at the bottom. A lead screw passes through the through hole, and the inner wall of the through hole has an internal thread that matches the external thread on the outer surface of the lead screw. When the lead screw rotates, it drives the laser light source along the length of the light source area through the base, irradiating the bottom of each sample bottle in stages.
[0011] Optionally, the laser source is an LED lamp with an emission spectral peak of 458 nm.
[0012] Optionally, the laser source is connected to a control circuit component via a circuit. The control circuit component is connected to a switch and a charging interface. The switch and charging interface are located on the outer wall of the wide side of the light source area, which facilitates switching on and off to excite the light source and charging the excitation light source.
[0013] Although the lead screw rotation can steplessly adjust the position of the base, theoretically allowing for individual alignment with the bottom of each sample vial, in practice, operators need to constantly observe the movement of the excitation light source through an observation room, which can easily cause eye fatigue. While some technologies and devices exist for controlling movement in the field of automation control, using these existing technologies not only increases equipment cost and overall weight but also occupies additional space within the light source area, sometimes necessitating its expansion. This invention provides a device for limiting and positioning the base using physical and mechanical methods; it has a simple structure and does not increase energy consumption.
[0014] Optionally, a positioning device is provided on the rear side of the light source area. The positioning device includes a support plate and several positioning plates. The support plate is vertically erected on the rear part of the light source area and is parallel to the rear side of the light source area. Along the length of the light source area, several vertical hollow strips are provided on the rear side of the light source area and the support plate, and the hollow strips on the rear side of the light source area and the support plate correspond one-to-one.
[0015] A positioning plate passes sequentially through the corresponding hollow strips on the rear side of the light source area and the support plate, and the positioning plate is perpendicular to the support plate. The positioning plate extends out of the front end of the support plate to position the limiting base.
[0016] Further optionally, the portion of the positioning plate located between the rear side of the light source area and the support plate is provided with a front positioning block and a rear positioning block, the front positioning block being close to the rear side of the support plate and the rear positioning block being close to the inner wall of the rear side of the light source area; both positioning blocks penetrate the positioning plate and both positioning blocks are horizontal.
[0017] The rear positioning block limits the position of the positioning plate so that its front end is always in front of the support plate; the front positioning block limits the position of the positioning plate so that its rear end is always behind the light source area.
[0018] Optionally, a vertical perforated strip is provided on the side wall between the observation chamber and the detection area for inserting a filter. The filter only allows emitted fluorescence with a wavelength greater than 510 nm to pass through.
[0019] Optionally, the bracket is located between the operation area and the detection area. The bracket is horizontally set along the length of the detection chamber. The rear side of the bracket is fixedly connected to the rear wall of the detection chamber. The front side of the bracket faces the observation chamber and is a continuous wave shape, wherein each backward-concave trough corresponds to a sample bottle.
[0020] The troughs correspond one-to-one with the moving parts. The front side of the trough is arc-shaped and vertical, and has a horizontal groove for connecting to the rear end of the moving parts. The troughs of the bracket play the role of limiting and supporting the moving parts.
[0021] Optionally, the movable frame is horizontal and has the same height as the bracket. The front half of the movable frame is located on the upper surface of the observation room ceiling and is supported by the upper surface.
[0022] The movable frame is formed by the side joints of a row of several movable parts, which are arranged in a single layer along the length of the testing chamber; the movable parts are long and rectangular and perpendicular to the length of the testing chamber.
[0023] Further optionally, the rear end of the moving component has an arc and size that adapts to the corresponding bracket trough groove, so that the rear end of the moving component can be inserted into the corresponding trough groove. When the rear wall of the groove abuts against the rear end of the moving component, the moving component cannot continue to move backward, and the bracket limits the movement of the moving component.
[0024] The moving part has a sample hole at the middle and rear for placing sample vials; the moving part has a handle at the front for easy pulling. Attached Figure Description
[0025] Figure 1 This is a three-dimensional schematic diagram of a portable universal optical inspection device (the moving frame is omitted).
[0026] Figure 2 This is a side view of the detection device;
[0027] Figure 3 This is a schematic diagram of the moving component;
[0028] Figure 4 This is a schematic diagram of the positioning device;
[0029] Figure 5 This is a schematic diagram showing the cooperation between the bracket and the movable frame;
[0030] Figure 6 This is a schematic diagram showing the connection between two adjacent moving parts.
[0031] Among them, 1-operation area, 2-detection area, 3-light source area, 4-observation chamber, 5-sample bottle, 6-interface, 7-bracket, 8-moving frame, 9-moving component, 10-base, 11-lead screw, 12-support plate, 13-positioning plate, 14-front positioning block, 15-rear positioning block, 16-trough, 17-sample hole, 18-connection strip, 19-groove. Detailed Implementation
[0032] This embodiment provides a portable, universal optical inspection device, such as... Figures 1-6As shown, the device includes a detection chamber and an observation chamber 4. The detection chamber, from top to bottom, includes an operation area 1, a detection area 2, and a light source area 3. The top of the operation area 1 has an openable and closable top cover for placing and removing sample vials 5 into the operation area 1 and the detection area 2. Each side of the detection chamber has an openable and closable interface 6 for connecting to other external optical detection devices to perform optical detection on the samples inside the detection chamber. An observation chamber 4 is located in front of the detection area 2, and a removable filter is provided between the detection area 2 and the observation chamber 4 for observing the fluorescence effect of the samples.
[0033] The testing chamber is equipped with a row of fixed brackets 7 and a row of movable frames 8. The movable frames 8 include several movable parts 9, which are used to place several sample bottles and can move the sample bottles individually to accept the light source of other optical testing devices.
[0034] A movable excitation source is provided in the light source area 3. The excitation source is located below the sample bottle and is used to illuminate the bottom of the sample bottle upwards for fluorescence detection.
[0035] Optionally, the three areas of the detection chamber are vertically connected, the three areas are of equal length, the operating area 1 and the detection area 2 are of equal width, the rear sides of the three areas are on the same vertical plane, and the front sides of the operating area 1 and the detection area 2 are on the same vertical plane, so that the operating area 1 and the detection area 2 are completely vertically aligned, making it easy for the sample bottle to pass through the operating area 1 and the detection area 2.
[0036] The width of the light source area 3 is greater than the width of the detection area 2. The front part of the light source area 3 is located below the observation chamber 4, so that the excitation light source and the matching moving components are located at the rear of the light source area 3, and the control circuit components are located at the front of the light source area 3.
[0037] Optionally, the front side of the observation chamber 4 is provided with an openable and closable cover to facilitate the observation of the fluorescence effect of the sample bottle in the detection area 2; the four sides and bottom of the light source area 3 are all closed.
[0038] Further optionally, the moving component includes a base 10 and a lead screw 11. The lead screw 11 is parallel to the length direction of the light source area 3. The two ends of the lead screw 11 extend through the two wide sides of the light source area 3 and are rotatably connected to the two wide sides of the light source area 3. Handwheels are provided at both ends of the lead screw 11 to facilitate the operator to hold the handwheels and rotate the lead screw 11.
[0039] The base 10 has a laser light source at the top and a through hole at the bottom. The lead screw 11 passes through the through hole. The inner wall of the through hole has an internal thread that matches the external thread on the outer surface of the lead screw 11. The lead screw 11 rotates, thereby driving the laser light source along the length of the light source area 3 through the base 10 to irradiate the bottom of each sample bottle in stages.
[0040] Alternatively, the laser source is an LED lamp with an emission spectrum peak of 458 nm (belonging to the blue light band). This wavelength can efficiently excite the fluorescent reporter groups commonly used in the CRISPR-Cas12a system, ensuring the generation of sufficient fluorescence signals while avoiding unnecessary thermal interference or optical damage to the sample.
[0041] Optionally, the laser source is connected to a control circuit component via wiring. The control circuit component is connected to a switch and a charging interface, which are located on the wide side outer wall of the front portion of the light source area 3, facilitating the switching on and off of the light source and charging of the light source. The control circuit component is a conventional control circuit component.
[0042] Optionally, a positioning device is provided on the rear side of the light source area 3. The positioning device includes a support plate 12 and several positioning plates 13. The support plate 12 is vertically erected on the rear part of the light source area 3 and close to the rear side of the light source area 3. The support plate 12 is parallel to the rear side of the light source area 3. Along the length direction of the light source area 3, several vertical hollow strips are provided on the rear side of the light source area 3 and the support plate 12, and the hollow strips on the rear side of the light source area 3 and the support plate 12 correspond one-to-one.
[0043] A positioning plate 13 passes sequentially through the rear side of the light source area 3 and the corresponding hollow strip on the support plate 12, and the positioning plate 13 is perpendicular to the support plate 12. The positioning plate 13 extends out of the front end of the support plate 12 to position the base 10.
[0044] Optionally, the positioning plate 13 corresponds one-to-one with the sample bottle, but the positions of the sample bottles and the positioning plate in the same group are staggered. When the positioning plate is inserted into the light source area to the limit position (i.e., it cannot be inserted any further), the positioning plate is forward (i.e., closer to the observation chamber side) than the other positioning plates. The positioning plate can intercept the base 10, so that the base 10 stops. At this time, the center of the excitation light source on the base 10 is directly below the center of the bottom of the corresponding sample bottle.
[0045] The portion of the positioning plate 13 located between the rear side of the light source area 3 and the support plate 12 is provided with a front positioning block 14 and a rear positioning block 15. The front positioning block 14 is close to the rear side of the support plate 12, and the rear positioning block 15 is close to the inner wall of the rear side of the light source area 3. Both positioning blocks penetrate the positioning plate 13 and are horizontal.
[0046] The limiting effect of the rear positioning block 15 ensures that the front end of the positioning plate 13 is always in front of the support plate 12; the limiting effect of the front positioning block 14 ensures that the rear end of the positioning plate 13 is always behind the light source area 3, that is, outside the light source area 3.
[0047] In use, all positioning plates 13 are pulled out until the rear positioning block 15 abuts against the inner wall of the rear side of the light source area 3. At this point, the positioning plates 13 extend the longest from the light source area 3, and the front ends of the positioning plates 13 do not contact the base 10, allowing the base 10 to move freely in the rear part of the light source area 3. If the base 10 moves from right to left, the position of each positioning plate 13 is offset to the left compared to the center position of the corresponding sample bottle. A positioning plate 13 is pushed into the light source area 3 (i.e., the positioning plate 13 moves forward) until the front positioning block 14 of that positioning plate 13 abuts against the rear side of the support plate 12. At this point, the front end of the positioning plate 13 is at its forwardmost position. When the left side of the base 10 moves to the front end of the positioning plate 13, it is blocked by the front end of the positioning plate 13 and cannot move forward. At this point, the center of the excitation light source is directly below the center of the bottom of the corresponding sample bottle, ensuring that the excitation light source is directly facing the target sample bottle, thereby obtaining the best fluorescence effect.
[0048] Since the rear half of the positioning plate 13 is located outside the detection chamber, it is easy to identify the positioning plate 13 corresponding to the target sample bottle to be detected by fluorescence. This makes it easy to control the target positioning plate 13 from outside the detection chamber. Without having to constantly observe the situation inside the observation chamber 4, the screw 11 can be rotated from the outside to move the excitation light source directly below the target sample bottle.
[0049] When performing fluorescence detection, the sample vial is preferably a conical plastic sample tube with a larger top and smaller bottom. This allows the excitation light to be precisely focused on the core reaction area of the solution inside the sample tube, effectively avoiding background fluorescence and scattered light induced by the excitation light irradiating the side wall of the sample tube (especially plastic material). This significantly reduces optical noise from the signal source, improves the detection signal-to-noise ratio, and reduces false positives.
[0050] Optionally, a vertical perforated strip is provided on the side wall between the observation chamber 4 and the detection area 2 for inserting a filter. The filter only allows emitted fluorescence with a wavelength greater than 510 nm to pass through, thereby accurately and thoroughly blocking and filtering out scattered light from the 458 nm excitation light and other short-wavelength stray light, improving the signal-to-noise ratio and detection specificity of the device.
[0051] The laser light source of this invention has an emission spectrum peak of 458 nm. Combined with the above-mentioned filter, it only allows emission fluorescence with wavelengths greater than 510 nm to pass through, thereby filtering out interference from the 458 nm excitation light. At the same time, it ensures that the fluorescence signals of commonly used fluorescent reporter molecules (with emission spectra usually in the 510-530 nm band) in the RPA-CRISPR-Cas12a system can be efficiently transmitted and detected. This precise matching design of photophysical properties ensures the optimization of excitation efficiency and fluorescence collection purity, which is the physical basis for realizing high-sensitivity naked-eye observation or mobile phone photography detection.
[0052] Optionally, the bracket 7 is located between the operation area 1 and the detection area 2. The bracket 7 is horizontally arranged along the length of the detection chamber. The rear side of the bracket 7 is fixedly connected to the rear wall of the detection chamber. The front side of the bracket 7 faces the observation chamber 4 and is a continuous wave shape, wherein each backward-recessed trough 16 corresponds to a sample bottle.
[0053] The troughs 16 correspond one-to-one with the moving parts 9. The front side of the trough 16 is arc-shaped and vertical, and has a horizontal groove 19 for connecting with the rear end of the moving parts 9. The troughs 16 of the bracket 7 serve to limit and support the moving parts 9. Several troughs are evenly distributed along the direction of the bracket.
[0054] Optionally, the movable frame 8 is horizontal and has the same height as the bracket 7. The front half of the movable frame 8 is located on the upper surface of the top surface of the observation chamber 4 and is supported by the upper surface.
[0055] The movable frame 8 is formed by the side joints of a row of several movable parts 9, and the several movable parts 9 are arranged in a single layer along the length of the detection chamber; the movable parts 9 are long strips and perpendicular to the length of the detection chamber.
[0056] Further optionally, the rear end of the moving part 9 has an arc and size that adapts to the groove of the corresponding bracket 7 trough 16, so that the rear end of the moving part 9 can be inserted into the groove of the corresponding trough 16. When the rear wall of the groove abuts against the rear end of the moving part 9, the moving part 9 cannot continue to move backward, and the bracket 7 limits the movement of the moving part 9.
[0057] The moving part 9 has a sample hole 17 at the middle and rear for placing sample bottles; the moving part 9 has a handle at the front for easy pulling.
[0058] Optionally, each moving component 9 has a mating strip 18 on the same side. The longitudinal section of the mating strip 18 is I-shaped. The sides of two adjacent moving components are respectively engaged in the grooves on both sides of the mating strip 18. The mating strip 18 serves as a moving track for the moving component 9, conceals the mating gap between adjacent moving components 9, and supports the moving component 9, maintaining the integrity of the moving frame 8. The mating strip 18 is a flexible rubber strip.
[0059] When fluorescence detection is performed using an excitation light source, the rear ends of all moving parts are inserted into the corresponding trough grooves, meaning all moving parts are at the very back and all sample vials are in the same row.
[0060] When other tests are required, the side of the testing chamber is connected to other optical testing instruments. The moving part 9 is used to pull a sample bottle to be tested forward so that light shines on the sample bottle. Depending on the difference in the detection principle of the optical testing instruments, the light can either return to the receiving device of the optical testing instrument or be projected and emitted through the interface on the other side of the testing chamber, and then be received by the relevant receiving device, thus completing the test.
[0061] In one specific implementation, a docking strip is fixedly provided on the left side edge of each moving component, while no docking strip is provided on the right side edge. Instead, the right side edge docks with the docking strip on the left side of the left moving component, so that the moving components dock with each other to form a moving frame.
[0062] Within detection zone 2, the movable frame 8 and the bracket 7 form the top surface of detection zone 2. By designing the groove depth of the trough 16 of the bracket 7 to be deeper, when the movable component 9 moves forward, its rear end never leaves the groove of the trough 16. Thus, the top surface formed by the movable frame 8 and the bracket 7 becomes a closed plane, preventing light from detection zone 2 from entering operation zone 1. At this point, the light can be prevented from entering observation zone 4 by replacing the filter with a light-shielding plate through the perforated strip on the side of observation chamber 4, thereby preserving as much light as possible within detection zone 2. Furthermore, it should be explained why this invention prevents light from detection zone 2 from entering observation chamber 4 and operation zone 1. Because the observation chamber 4 and the operation area 1 are the two areas in this detection device that have open sides to the outside, while the light source area 3 is closed, and this detection device is portable and has a small overall volume (e.g., a few cubic decimeters or tens of cubic centimeters), the volume of the light source area 3 is also not large. Moreover, only the rear half of the light source area 3 directly corresponds to the detection area 2, and the rear half of the light source area 3 has a right-angle bend with the detection area 2, so the light energy loss is limited.
[0063] When other optical instruments are used for spectrophotometry, the sample vials need to be replaced with cuvettes. Even when the sample orifice of the moving part is circular, the cuvette can still be stably placed.
[0064] Other optical detection instruments of the present invention include, but are not limited to, spectrophotometers, fluorescence microplate readers, and real-time quantitative PCR instruments.
Claims
1. A portable, universal optical inspection device, characterized in that, It includes a detection chamber and an observation chamber. The detection chamber consists of an operating area, a detection area, and a light source area from top to bottom. The top of the operating area has an openable and closable cover for placing and removing sample vials into the operating area and the detection area. There is an openable and closable interface on each side of the detection chamber for connecting to other external optical detection devices to perform optical detection on the samples inside the detection chamber. An observation chamber is located in front of the detection area, and a removable filter is installed between the detection area and the observation chamber for observing the fluorescence effect of the samples. The testing chamber is equipped with a row of fixed brackets and a row of movable racks. The movable racks include several moving parts for placing several sample bottles and can move the sample bottles individually for testing. A movable excitation source is provided in the light source area. The excitation source is located below the sample bottle and is used to illuminate the bottom of the sample bottle upwards for fluorescence detection.
2. The portable universal optical inspection device according to claim 1, characterized in that, The three areas of the detection chamber are vertically connected, with equal lengths, equal widths of the operation area and the detection area, and the rear sides of the three areas on the same vertical plane. The front sides of the operation area and the detection area are on the same vertical plane, making the operation area and the detection area completely vertically aligned, which facilitates the passage of sample vials through the operation area and the detection area. The width of the light source area is greater than the width of the detection area. The front part of the light source area is located below the observation chamber. The excitation light source and its associated moving components are located at the rear of the light source area, while the control circuit components are located at the front of the light source area.
3. The portable universal optical inspection device according to claim 2, characterized in that, The movable component includes a base and a lead screw. The lead screw is parallel to the length direction of the light source area, and its two ends extend through the two wide sides of the light source area, making it convenient for the operator to rotate the lead screw. The base has a laser light source at the top and a through hole at the bottom. A lead screw passes through the through hole, and the inner wall of the through hole has an internal thread that matches the external thread on the outer surface of the lead screw. When the lead screw rotates, it drives the laser light source along the length of the light source area through the base, irradiating the bottom of each sample bottle in stages.
4. The portable universal optical inspection device according to claim 2, characterized in that, The laser source is an LED lamp with an emission spectrum peak of 458 nm; The laser source is connected to the control circuit components via a circuit. The control circuit components are connected to a switch and a charging interface. The switch and charging interface are located on the outer wall of the wide side of the light source area, which facilitates the switching on and off of the light source and the charging of the light source.
5. The portable universal optical inspection device according to claim 1, characterized in that, The rear side of the light source area is provided with a positioning device, which includes a support plate and several positioning plates. The support plate is vertically erected in the rear part of the light source area and is parallel to the rear side of the light source area. Along the length of the light source area, the rear side of the light source area and the support plate are provided with several vertical hollow strips, and the hollow strips on the rear side of the light source area and the support plate correspond one-to-one. A positioning plate passes sequentially through the corresponding hollow strips on the rear side of the light source area and the support plate, and the positioning plate is perpendicular to the support plate. The positioning plate extends out of the front end of the support plate to position the limiting base.
6. The portable universal optical inspection device according to claim 5, characterized in that, The portion of the positioning plate located between the rear side of the light source area and the support plate is provided with a front positioning block and a rear positioning block. The front positioning block is close to the rear side of the support plate, and the rear positioning block is close to the inner wall of the rear side of the light source area. Both positioning blocks penetrate the positioning plate and are horizontal. The rear positioning block limits the position of the positioning plate so that its front end is always in front of the support plate; the front positioning block limits the position of the positioning plate so that its rear end is always behind the light source area.
7. The portable universal optical inspection device according to claim 2, characterized in that, A vertical perforated strip is provided on the side wall between the observation chamber and the detection area for inserting a filter. The filter only allows emitted fluorescence with a wavelength greater than 510 nm to pass through.
8. The portable universal optical inspection device according to claim 2, characterized in that, The bracket is located between the operation area and the detection area. The bracket is horizontally set along the length of the detection chamber. The rear side of the bracket is fixedly connected to the rear wall of the detection chamber. The front side of the bracket faces the observation chamber and is a continuous wave shape, where each backward-concave trough corresponds to a sample bottle. The troughs correspond one-to-one with the moving parts. The front side of the trough is arc-shaped and has a horizontal groove for connecting to the rear end of the moving parts. The troughs of the bracket play the role of limiting and supporting the moving parts.
9. The portable universal optical inspection device according to claim 8, characterized in that, The movable frame is horizontal and its height is the same as that of the bracket. The front half of the movable frame is located on the upper surface of the observation room ceiling and is supported by the upper surface. The movable frame is formed by the side joints of a row of several movable parts, which are arranged in a single layer along the length of the testing chamber; the movable parts are long and rectangular and perpendicular to the length of the testing chamber.
10. The portable universal optical inspection device according to claim 9, characterized in that, The rear end of the moving component has an arc and size that adapts to the corresponding bracket trough groove, making it easy for the rear end of the moving component to be inserted into the corresponding trough groove. When the rear wall of the groove abuts against the rear end of the moving component, the moving component cannot continue to move backward, and the bracket limits the movement of the moving component. The moving part has a sample hole at the middle and rear for placing sample vials; the moving part has a handle at the front for easy pulling.