A device and method for quantitatively detecting a metabolite of lactic acid bacteria

Abstract

The application belongs to the technical field of metabolite detection, in particular to a lactic acid bacteria metabolite quantitative detection device and method, which comprises a liquid chromatograph analyzer, a controller is fixedly arranged on the side of the liquid chromatograph analyzer, a placing box is arranged on the top of the liquid chromatograph analyzer, a limiting ring is arranged on a bearing block, a supporting shaft is rotatably arranged in the limiting ring, a power motor is fixedly arranged in the bearing block, a fixed ring is circumferentially arranged on the supporting shaft, a storage cylinder is fixedly arranged in the fixed ring, a liquid delivery pipe is connected to the bottom of the storage cylinder, and a stirring mechanism is arranged in the storage cylinder. The lactic acid bacteria metabolite quantitative detection device and method can realize rapid sample replacement detection through the arrangement of the fixed ring and the storage cylinder, effectively improve the flexibility of equipment use, continuously adjust the position of the storage cylinder after completing the detection of a single group of samples, make the next storage cylinder sequentially align with the conical groove and repeat the above process, and thus quickly complete the rotation inspection of different samples, and significantly improve the overall inspection efficiency.

Description

Technical Field

[0001] This invention belongs to the field of metabolite detection technology, specifically a quantitative detection device and method for lactic acid bacteria metabolites. Background Technology

[0002] The quantitative detection device for lactic acid bacteria metabolites is mainly used for qualitative identification and precise quantitative analysis of metabolites such as lactic acid and acetic acid in lactic acid bacteria fermentation broth. The device is based on a liquid chromatography analyzer. During the detection process, the instrument distinguishes different components through chromatographic separation, determines the types of metabolites by combining retention time, and calculates the content of substances based on peak area. It is widely used in quality control of lactic acid bacteria fermentation process, research on the metabolic characteristics of strains, and inspection of fermentation products, providing reliable data for process optimization, scientific research experiments, and product quality control.

[0003] A Chinese patent with publication number CN121049421A discloses a method and apparatus for detecting the residues of tetrazolium acetamiprid and its metabolites. The method includes the following steps: Step 1, dissolving the tetrazolium acetamiprid and its metabolite sample in an extraction solvent, and then injecting the extract into a solvent bottle on a liquid chromatograph; Step 2, inserting the connector into the bottle head at the top of the solvent bottle, connecting the connector with the straight tubing inside the solvent bottle, magnetically connecting the second magnetic ring on the connector with the first magnetic ring on the bottle head, and then connecting the first infusion tube on the connector with the docking part on the hollow block to complete the assembly of a section of the pipeline structure. This invention, through segmented assembly of the infusion pipeline, magnetic ring limiting, and segmented pipe fitting design, effectively enhances the stability of the pipeline connection, avoids leakage, ensures continuous and accurate chromatographic analysis experiments, reduces the risk of experimental failure, and reduces solvent waste and environmental pollution.

[0004] Existing quantitative detection devices for lactic acid bacteria metabolites are cumbersome to operate during sample testing, making it difficult to quickly switch between different samples and continuously inject samples. This not only significantly increases the auxiliary time for a single test, directly reducing the overall testing efficiency, but also fails to meet the operational needs of centralized testing of large batches of fermentation samples. In addition, the long waiting time for samples to be tested can easily lead to bacterial sedimentation, adsorption of effective components, and continuous metabolism of microorganisms, further causing deviations in test results and bringing many inconveniences to daily testing quality control.

[0005] Therefore, the present invention provides a device and method for quantitative detection of lactic acid bacteria metabolites. Summary of the Invention

[0006] To overcome the shortcomings of existing technologies and solve the problem of inconvenient sample replacement for testing, this invention proposes a quantitative detection device and method for lactic acid bacteria metabolites.

[0007] The technical solution adopted by the present invention to solve its technical problem is as follows: A quantitative detection device for lactic acid bacteria metabolites, comprising a liquid chromatograph, a controller fixedly mounted on the side of the liquid chromatograph, a placement box mounted on the top of the liquid chromatograph, a storage bottle for rinsing the liquid chromatograph inside the placement box, an injector mounted on the other side of the liquid chromatograph, a sample dispensing head mounted on the injector, a communication mechanism for liquid supply mounted on the side of the liquid chromatograph, a mounting plate fixedly mounted on the side of the mounting plate, a load-bearing block mounted on the side of the load-bearing block, a limit ring mounted on the load-bearing block, a support shaft rotatably mounted inside the limit ring, a power motor fixedly mounted inside the load-bearing block, and the output end of the power motor fixedly connected to the end of the support shaft, a fixing ring circumferentially mounted on the support shaft, a storage cylinder fixedly mounted inside the fixing ring, a liquid infusion tube connected to the bottom of the storage cylinder, and a stirring mechanism mounted inside the storage cylinder.

[0008] By adopting the above scheme, when using a quantitative detection device for lactic acid bacteria metabolites for quantitative detection, the liquid chromatography analyzer completes the sample analysis. After the sample pretreatment is completed, the liquid chromatography analyzer is started and preheated. The column is continuously flushed with mobile phase until the instrument baseline remains stable. Then, a blank sample is injected for detection to confirm that there are no interfering peaks in the chromatogram. To reduce the detection error caused by instrument signal drift, the standard, the sample to be tested, and the standard are injected in the correct order. Each sample is injected repeatedly through the injector, and the average value is taken to effectively improve the repeatability of the results. The liquid chromatography analyzer automatically collects and records the retention time and peak area of ​​each group of samples. The retention time of the sample to be tested is compared with that of the standard to determine the metabolite components, and finally the quantitative detection of lactic acid bacteria metabolites is achieved. When multiple samples need to be tested, different samples are prepared in advance and stored in the storage cylinder. When automatic adjustment is required for testing different samples, the connecting mechanism, telescopic hose and sample head work together to make the injector and the standard... The conical grooves are interconnected. Controlling the motor drives the support shaft to rotate. As the support shaft rotates, it moves the storage cylinder via a fixing ring. Once the storage cylinder containing the sample to be tested is positioned directly above the conical groove, the solenoid valve on the infusion tube is activated to deliver the sample into the conical groove. Through the connecting mechanism, telescopic hose, and sample dispensing head, the sample enters the liquid chromatograph for analysis. When changing samples, the liquid chromatograph is first cleaned. Then, the motor is activated again, and the position of the storage cylinder is adjusted via the support shaft and fixing ring, moving it directly above the conical groove. Repeating this process allows for quick sample replacement, improving testing efficiency.

[0009] Preferably, the connecting mechanism includes a load-bearing plate, a strip groove, a servo motor, a threaded rod, a sliding plate, and a connecting pipe. The strip groove is fixedly provided on the load-bearing plate, and the servo motor is fixedly provided at the end of the strip groove. The threaded rod is rotatably provided inside the strip groove, and one end of the threaded rod is fixedly connected to the output end of the servo motor. The sliding plate passes through the strip groove, and the outer surface of the threaded rod is threadedly connected to the inside of the sliding plate. The connecting pipe is fixed inside the sliding plate, and one end of the connecting pipe is fixedly connected to an infusion needle.

[0010] By adopting the above scheme, the servo motor is controlled to drive the threaded rod to rotate, thereby adjusting the position of the slide plate. When the slide plate moves, it drives the connecting tube to move, and the connecting tube moves, which in turn drives the infusion needle to move. The infusion needle can be inserted into the sample feeding head to input the test sample.

[0011] Preferably, the slide plate is symmetrically provided with guide grooves, and the strip groove is fixedly provided with guide blocks corresponding to the guide grooves, and the guide blocks are engaged with the corresponding guide grooves. The other end of the connecting pipe is connected to a telescopic hose.

[0012] By adopting the above scheme, when adjusting the position of the slide plate, the guide groove and guide block work together to guide the slide plate, so that the slide plate moves smoothly. The telescopic hose is telescopic, and it is convenient to transport samples into the connecting pipe through the telescopic hose.

[0013] Preferably, the liquid chromatograph is fixedly provided with a receiving tank, an adjusting plate is provided inside the receiving tank, a cavity is provided inside the adjusting plate, and an electric push rod is fixedly provided inside the receiving tank, with the telescopic end of the electric push rod fixedly connected to the inner wall of the cavity.

[0014] By adopting the above scheme, the operation of the electric push rod can drive the adjustment plate to move. The top of the adjustment plate is fixedly connected to the bottom of the load-bearing plate. When the adjustment plate moves, the position of the load-bearing plate can be adjusted. Furthermore, through the cooperation of the strip groove, the vertical position of the connecting tube can be adjusted. After the connecting tube moves, it is no longer located on one side of the sample injector, and the sample can be manually injected. By adjusting the vertical position of the connecting tube, the sample delivery for single inspection or multiple inspections can be flexibly switched.

[0015] Preferably, a liquid storage tank is fixedly installed inside the mounting plate, a liquid addition pipe is installed on the liquid storage tank, a pump is fixedly installed inside the liquid storage tank, the input end of the pump extends into the liquid storage tank, and the output end of the pump is connected to a connecting pipe.

[0016] By adopting the above scheme, cleaning fluid can be easily added to the storage tank through the filling pipe, and the cleaning fluid in the storage tank can be extracted by controlling the operation of the pump. The extracted cleaning fluid will flow into the connecting pipe for transportation.

[0017] Preferably, a plate is fixedly installed on the side of the liquid chromatograph, a conical groove is fixedly installed on the plate, a delivery pump is fixedly installed inside the plate, the input end of the delivery pump is connected to the conical groove, and the output end of the delivery pump is fixedly connected to the other end of the telescopic hose.

[0018] By adopting the above scheme, the liquid chromatograph provides installation space for the conical trough through the plate. After the liquid sample or cleaning solution enters the conical trough, the delivery pump works to draw and deliver the liquid flow. The liquid flow is allowed to enter the connecting tube through the telescopic hose. The cleaning solution enters the connecting tube to rinse it. After the connecting tube is cleaned, the sample is added into the conical trough for delivery. The infusion needle is connected to the injector to deliver the sample for testing.

[0019] Preferably, a diversion groove is embedded in the conical groove, and the other end of the connecting pipe is connected to the diversion groove, and spray holes are arranged in an array on the diversion groove.

[0020] By adopting the above scheme, the liquid pumped by the delivery pump flows into the diversion tank, and the liquid flows into the conical tank through the spray hole to rinse and clean the conical tank.

[0021] Preferably, a special-shaped frame is fixedly provided on the side of the mounting plate, a sealing cap is threadedly connected to the top of the storage cylinder, and a liquid filling head is fixedly provided on the sealing cap.

[0022] By adopting the above solution, the mounting plate provides installation space for irregularly shaped racks, and the liquid filling head facilitates the adjustment of temporary sample storage in the storage cylinder. The sealing cap is removable.

[0023] Preferably, the stirring mechanism includes a rotating shaft, stirring blades, and a driven gear. The rotating shaft is rotatably disposed inside the sealing cover, the stirring blades are fixed on the rotating shaft, and one end of the rotating shaft is fixedly connected to the center position of the driven gear.

[0024] By adopting the above scheme, the driven gear will rotate, which will drive the rotating shaft to rotate. The rotation of the rotating shaft will drive the stirring blade to move. The circular motion of the stirring blade will mix and stir the sample stored inside the storage cylinder, thereby effectively avoiding the formation of sediment when the sample is left to stand, which would affect the accuracy of the sample test.

[0025] Preferably, a gear ring is fixedly installed inside the irregular frame, and the gear ring meshes with the driven gear.

[0026] By adopting the above scheme, when the power motor is working, the position of the storage cylinder will be adjusted through the cooperation of the support shaft and the fixed ring. The circumferential motion of the storage cylinder will drive the driven gear to move. Through the cooperation of the gear ring, the driven gear will rotate, which in turn will drive the rotating shaft to rotate.

[0027] A method for detecting lactic acid bacteria metabolites using a quantitative detection device includes the following steps: S1. Start the liquid chromatograph and preheat it. Purge the column with the mobile phase until the instrument baseline remains stable. S2. Then, inject blank samples for testing to confirm that there are no interfering peaks in the spectrum. In order to reduce the detection error caused by instrument signal drift, strictly follow the order of standard, test sample, standard and inject them in sequence. Repeat the injection of each sample 1-2 times through the injector and take the average value of the test to effectively improve the repeatability of the results. S3. The liquid chromatograph automatically collects and records the retention time and peak area of ​​each group of samples, compares the retention time of the sample to be tested with that of the standard, determines the metabolite components, and finally realizes the quantitative detection of lactic acid bacteria metabolites.

[0028] The beneficial effects of this invention are as follows: 1. The present invention provides a quantitative detection device and method for lactic acid bacteria metabolites. By setting a fixing ring and a storage cylinder, it enables rapid sample replacement and detection, effectively improving the flexibility of equipment use. Before testing multiple samples, each set of samples to be tested can be prepared in advance. The samples are added to the storage cylinder using a dispensing head for temporary storage. When automatic sample switching is required, the power motor is started, which drives the support shaft to rotate. During the rotation of the support shaft, the storage cylinder moves as a whole through the fixing ring. When the storage cylinder containing the sample to be tested rotates to the top of the conical groove, the solenoid valve on the infusion tube is opened, and the sample can be delivered into the conical groove for detection. After the detection of a single set of samples is completed, the position of the storage cylinder is continuously adjusted so that the next storage cylinder is aligned with the conical groove and the above process is repeated, which can quickly complete the rotation testing of different samples and significantly improve the overall testing efficiency.

[0029] 2. The quantitative detection device and method for lactic acid bacteria metabolites described in this invention, by equipping a stirring blade structure, can continuously stir the sample in the storage cylinder, effectively preventing precipitation after the sample has settled, ensuring the accuracy of the detection results. When conducting multiple sample detection operations, the power motor drives the support shaft and the fixed ring to rotate, completing the position switching of the storage cylinder. While the storage cylinder is making circular motion, it drives the driven gear to move along the circumference of the gear ring and engages in transmission, driving the driven gear to rotate, which in turn drives the rotating shaft to rotate synchronously. The rotating shaft further drives the stirring blade to make circular motion, thoroughly mixing and stirring the sample inside the storage cylinder, preventing the formation of precipitates from the source, eliminating the interference of impurities on the detection results, and ensuring stable and reliable sample test data.

[0030] 3. The quantitative detection device and method for lactic acid bacteria metabolites described in this invention, by configuring a pump and a diversion tank, can clean the sample delivery pipeline and cavity, effectively avoiding cross-contamination between different samples during sample replacement. When switching samples to be tested, the pump operates to extract cleaning solution, which flows into the diversion tank and is then sprayed into the conical tank through the spray nozzle to complete the rinsing and cleaning of the conical tank. The cleaning solution continues to flow along the passage, sequentially flowing through the telescopic hose, connecting tube and infusion needle, to achieve a comprehensive cleaning of the entire delivery pipeline, thoroughly removing residual materials in the channel and avoiding cross-contamination of samples during sample replacement from the source. Attached Figure Description

[0031] The invention will now be further described with reference to the accompanying drawings.

[0032] Figure 1 This is a perspective view of the quantitative detection device for lactic acid bacteria metabolites of the present invention; Figure 2 This is the present invention. Figure 1 A magnified structural diagram of A in the middle; Figure 3 This is a schematic diagram of the gear ring structure in this invention; Figure 4 This is a schematic diagram of the storage cylinder in this invention; Figure 5 This is a schematic diagram of the strip groove structure in this invention; Figure 6 This is a schematic diagram of the structure of the adjusting plate in this invention; Figure 7 This is a schematic diagram of the structure of the fixing ring and the support shaft in this invention.

[0033] In the diagram: 1. Liquid Chromatography Analyzer; 2. Controller; 3. Placement Box; 4. Storage Bottle; 5. Injector; 6. Sample Dispenser; 7. Storage Tank; 8. Adjustment Plate; 9. Cavity; 10. Electric Push Rod; 11. Load-bearing Plate; 12. Strip Groove; 13. Servo Motor; 14. Threaded Rod; 15. Slide Plate; 16. Guide Groove; 17. Guide Block; 18. Connecting Tube; 19. Infusion Needle; 20. Telescopic Hose; 21. Mounting Plate; 22. Storage 23. Liquid tank; 24. Liquid filling pipe; 25. Pump; 26. Connecting pipe; 27. Plate; 28. Conical groove; 29. ​​Transfer pump; 30. Diverter; 31. Loading block; 32. Limiting ring; 33. Support shaft; 34. Power motor; 35. Fixing ring; 36. Irregular frame; 37. Storage cylinder; 38. Liquid delivery pipe; 39. Sealing cap; 40. Liquid filling head; 41. Rotating shaft; 42. Stirring blade; 43. Driven gear; 44. Gear ring. Detailed Implementation

[0034] To make the technical means, creative features, objectives and effects of this invention easier to understand, the invention will be further described below in conjunction with specific embodiments.

[0035] like Figures 1 to 7 As shown in the embodiment of the present invention, a quantitative detection device for lactic acid bacteria metabolites includes a liquid chromatograph 1, a controller 2 fixedly mounted on the side of the liquid chromatograph 1, a storage box 3 mounted on the top of the liquid chromatograph 1, a storage bottle 4 for rinsing the liquid chromatograph 1 inside the storage box 3, an injector 5 mounted on the other side of the liquid chromatograph 1, a sample tip 6 mounted on the injector 5, and a communication mechanism for liquid supply mounted on the side of the liquid chromatograph 1. A mounting plate 21 is fixedly installed on the side, and a load-bearing block 30 is installed on the side of the mounting plate 21. A limit ring 31 is installed on the load-bearing block 30, and a support shaft 32 is rotatably installed inside the limit ring 31. A power motor 33 is fixedly installed inside the load-bearing block 30, and the output end of the power motor 33 is fixedly connected to the end of the support shaft 32. A fixing ring 34 is installed in a circumferential array on the support shaft 32. A storage cylinder 36 is fixedly installed inside the fixing ring 34. An infusion tube 37 is connected to the bottom of the storage cylinder 36, and a stirring mechanism is installed inside the storage cylinder 36.

[0036] The controller 2 is electrically connected to the electronic equipment on the quantitative detection device for lactic acid bacteria metabolites, and can control the operation of the quantitative detection device for lactic acid bacteria metabolites. The liquid chromatograph 1 has a built-in power distribution component, and an external power supply can provide power for the operation of the electronic equipment.

[0037] When using a quantitative detection device for lactic acid bacteria metabolites, the liquid chromatograph 1 performs sample analysis. After the sample pretreatment is completed, the liquid chromatograph 1 is started and preheated. The column is continuously flushed with mobile phase until the instrument baseline remains stable. A blank sample is then injected for detection to confirm the absence of interfering peaks in the chromatogram. To minimize detection errors caused by instrument signal drift, the samples are injected sequentially in the order of standard, test sample, and standard. Each sample is injected 1-2 times using the injector 5, and the average value is taken to effectively improve the repeatability of the results. The liquid chromatograph 1 automatically collects and records the retention time and peak area of ​​each group of samples. The retention time of the test sample is compared with that of the standard to determine the metabolite components, ultimately achieving the quantitative detection of lactic acid bacteria metabolites.

[0038] When multiple samples need to be tested, different samples are prepared in advance and stored inside the storage cylinder 36. When automatic adjustment is required for testing different samples, the connecting mechanism, telescopic hose 20, and sample dispensing head 6 connect the sample injector 5 and the conical groove 27. Controlling the power motor 33 drives the support shaft 32 to rotate. When the support shaft 32 rotates, it moves the storage cylinder 36 through the fixing ring 34, moving the storage cylinder 36 containing the samples to be tested directly above the conical groove 27. Then, controlling the solenoid valve on the infusion tube 37 allows the sample to be dispensed. The sample is conveyed into the conical groove 27. Through the connection mechanism, the telescopic hose 20 and the sample dispensing head 6, the sample to be tested enters the liquid chromatograph 1 for analysis. When it is necessary to change to a different sample, the liquid chromatograph 1 is first cleaned. Then, the power motor 33 is controlled to work again. Through the cooperation of the support shaft 32 and the fixing ring 34, the position of the storage cylinder 36 is adjusted so that the storage cylinder 36 is moved directly above the conical groove 27. The above operation is repeated to quickly change to a different sample for testing, thereby improving the efficiency of the testing work.

[0039] Furthermore, the connecting mechanism includes a load-bearing plate 11, a strip groove 12, a servo motor 13, a threaded rod 14, a sliding plate 15, and a connecting pipe 18. The strip groove 12 is fixedly installed on the load-bearing plate 11, and the servo motor 13 is fixedly installed at the end of the strip groove 12. The threaded rod 14 is rotatably installed inside the strip groove 12, and one end of the threaded rod 14 is fixedly connected to the output end of the servo motor 13. The sliding plate 15 passes through the strip groove 12, and the outer surface of the threaded rod 14 is threadedly connected to the inside of the sliding plate 15. The connecting pipe 18 is fixed inside the sliding plate 15, and one end of the connecting pipe 18 is fixedly connected to an infusion needle 19.

[0040] The operation of the servo motor 13 will drive the threaded rod 14 to rotate, thereby adjusting the position of the slide plate 15. When the slide plate 15 moves, it will drive the connecting tube 18 to move. When the connecting tube 18 moves, it will drive the infusion needle 19 to move. The infusion needle 19 can be inserted into the sample feeding head 6 to input the test sample.

[0041] Furthermore, guide grooves 16 are symmetrically arranged on the slide plate 15, and guide blocks 17 corresponding to the guide grooves 16 are fixedly arranged on the strip grooves 12. The guide blocks 17 are engaged with the corresponding guide grooves 16, and the other end of the connecting pipe 18 is connected to a telescopic hose 20.

[0042] When adjusting the position of the slide plate 15, the guide groove 16 and the guide block 17 work together to guide the slide plate 15, so that the slide plate 15 moves smoothly. The telescopic hose 20 is telescopic, and the sample can be easily transported into the connecting pipe 18 through the telescopic hose 20.

[0043] Furthermore, the liquid chromatograph 1 is fixedly provided with a receiving tank 7, an adjusting plate 8 is provided inside the receiving tank 7, a cavity 9 is provided inside the adjusting plate 8, and an electric push rod 10 is fixedly provided inside the receiving tank 7, with the telescopic end of the electric push rod 10 fixedly connected to the inner wall of the cavity 9.

[0044] The operation of the electric push rod 10 can push the adjustment plate 8 to move. The top of the adjustment plate 8 is fixedly connected to the bottom of the load-bearing plate 11. When the adjustment plate 8 moves, the position of the load-bearing plate 11 can be adjusted. In turn, through the cooperation of the strip groove 12, the vertical position of the connecting tube 18 can be adjusted. After the connecting tube 18 moves, it is no longer located on one side of the sample injector 5, and the sample can be manually injected. By adjusting the vertical position of the connecting tube 18, the sample delivery for single inspection or multiple inspections can be flexibly switched.

[0045] Furthermore, a liquid storage tank 22 is fixedly installed inside the mounting plate 21, a liquid addition pipe 23 is installed on the liquid storage tank 22, a pump 24 is fixedly installed inside the liquid storage tank 22, and the input end of the pump 24 extends into the liquid storage tank 22, and the output end of the pump 24 is connected to a connecting pipe 25.

[0046] Cleaning fluid can be easily added to the storage tank 22 via the filling pipe 23. The pump 24 can be controlled to extract the cleaning fluid from the storage tank 22. The extracted cleaning fluid will flow into the connecting pipe 25 for delivery.

[0047] Furthermore, a plate 26 is fixedly installed on the side of the liquid chromatograph 1, a conical groove 27 is fixedly installed on the plate 26, a transfer pump 28 is fixedly installed inside the plate 26, and the input end of the transfer pump 28 is connected to the conical groove 27, and the output end of the transfer pump 28 is fixedly connected to the other end of the telescopic hose 20.

[0048] The liquid chromatograph 1 provides installation space for the conical trough 27 via the plate 26. After the liquid sample or cleaning solution enters the conical trough 27, the delivery pump 28 works to draw and deliver the liquid flow. The liquid flow is made into the connecting pipe 18 through the telescopic hose 20. The cleaning solution enters the connecting pipe 18 to rinse it (a container for storing waste liquid can be set on one side). After the connecting pipe 18 is cleaned, the sample is added into the conical trough 27 for delivery. The infusion needle 19 is connected to the sample injector 5 to deliver the sample for testing.

[0049] Furthermore, a diversion groove 29 is embedded in the conical groove 27, and the other end of the connecting pipe 25 is connected to the diversion groove 29. Spray holes are arranged in an array on the diversion groove 29.

[0050] The liquid pumped by the delivery pump 28 flows into the diversion tank 29 and flows into the conical tank 27 through the spray hole, rinsing and cleaning the conical tank 27.

[0051] Furthermore, a special-shaped frame 35 is fixedly installed on the side of the mounting plate 21, and a sealing cap 38 is threadedly connected to the top of the storage cylinder 36. A liquid filling head 39 is fixedly installed on the sealing cap 38.

[0052] Mounting plate 21 provides installation space for irregular frame 35, and liquid filling head 39 facilitates the adjustment of temporary sample storage in storage cylinder 36. Sealing cap 38 is removable.

[0053] Furthermore, the stirring mechanism includes a rotating shaft 40, a stirring blade 41, and a driven gear 42. The rotating shaft 40 is rotatably disposed inside the sealing cover 38, the stirring blade 41 is fixed on the rotating shaft 40, and one end of the rotating shaft 40 is fixedly connected to the center position of the driven gear 42.

[0054] When the driven gear 42 rotates, it drives the rotating shaft 40 to rotate. The rotation of the rotating shaft 40 drives the stirring blade 41 to move. The circular motion of the stirring blade 41 mixes and stirs the sample stored inside the storage cylinder 36, which can effectively prevent the formation of sediment when the sample is left to stand, thus affecting the accuracy of sample testing.

[0055] Furthermore, a gear ring 43 is fixedly installed inside the irregular frame 35, and the gear ring 43 meshes with the driven gear 42.

[0056] When the power motor 33 is working, the position of the storage cylinder 36 will be adjusted through the cooperation of the support shaft 32 and the fixed ring 34. The circumferential movement of the storage cylinder 36 will drive the driven gear 42 to move. Through the cooperation of the gear ring 43, the driven gear 42 will rotate, which in turn will drive the rotating shaft 40 to rotate.

[0057] Working principle: First, when using the quantitative detection device for lactic acid bacteria metabolites to carry out quantitative detection, the liquid chromatograph 1 completes the sample analysis. After the sample pretreatment is completed, the liquid chromatograph 1 is started and preheated. The mobile phase is continuously introduced to flush the chromatographic column until the instrument baseline remains stable.Subsequently, blank samples were injected for testing to confirm the absence of interfering peaks in the chromatogram. To minimize detection errors caused by instrument signal drift, samples were injected sequentially in the order of standard, test sample, and standard. Each sample was injected 1-2 times using injector 5, and the average value was taken to effectively improve the repeatability of the results. The liquid chromatograph 1 automatically collected and recorded the retention time and peak area of ​​each group of samples. The retention time of the test sample was compared with that of the standard to determine the metabolite components, ultimately achieving quantitative detection of lactic acid bacteria metabolites. When testing multiple samples, different samples were prepared in advance and added to the storage cylinder 36 through the liquid dispensing head 39 for storage. Automatic adjustment is required. When testing different samples, the servo motor 13 drives the threaded rod 14 to rotate, thereby adjusting the position of the slide plate 15. The movement of the slide plate 15 moves the connecting tube 18, which in turn moves the infusion needle 19. The infusion needle 19 can be inserted into the sample feeding head 6 to input the sample for testing. Then, the power motor 33 drives the support shaft 32 to rotate. The rotation of the support shaft 32, through the fixing ring 34, moves the storage cylinder 36, moving it to directly above the conical groove 27. Then, the solenoid valve on the infusion tube 37 is activated to deliver the sample into the conical groove 27. The delivery pump 28 then draws and delivers the liquid flow through the telescopic hose. 20 will cause liquid to flow into the connecting tube 18, connecting the infusion needle 19 to the sample injector 5, allowing sample delivery for testing. When changing to a different sample, first clean the liquid chromatograph 1, then control the power motor 33 to work again. Through the cooperation of the support shaft 32 and the fixing ring 34, the position of the storage cylinder 36 will be adjusted, moving the storage cylinder 36 directly above the conical groove 27. Repeat the above operation to quickly change to a different sample for testing, improving the efficiency of the testing work. When the power motor 33 is working, through the cooperation of the support shaft 32 and the fixing ring 34, the position of the storage cylinder 36 will be adjusted. The circumferential movement of the storage cylinder 36 will drive the driven gear 42 to move. When the gear ring 43 is engaged, the driven gear 42 will rotate, which in turn will drive the rotating shaft 40 to rotate. The rotation of the rotating shaft 40 will drive the stirring blade 41 to move. The circumferential motion of the stirring blade 41 will mix and agitate the sample stored inside the storage cylinder 36, which can effectively prevent the formation of sediment when the sample is left to stand, thus affecting the accuracy of the sample test. When changing the sample transport, the liquid drawn by the transport pump 28 flows into the diversion tank 29. Through the spray hole, the liquid will flow into the conical tank 27 to rinse and clean the conical tank 27. After the cleaning fluid flows into the telescopic hose 20, the connecting pipe 18 and the infusion needle 19, it can be cleaned, which can effectively prevent cross-contamination when changing the sample for testing.

[0058] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of the present invention is defined by the appended claims and their equivalents.

Claims

1. A quantitative detection device for lactic acid bacteria metabolites, characterized in that: The system includes a liquid chromatograph (1), a controller (2) is fixedly installed on the side of the liquid chromatograph (1), a storage box (3) is installed on the top of the liquid chromatograph (1), a storage bottle (4) for rinsing the liquid chromatograph (1) is installed in the storage box (3), and an injector (5) is installed on the other side of the liquid chromatograph (1), with a sample dispensing head (6) installed on the injector (5). The liquid chromatograph (1) is provided with a communication mechanism for liquid supply on its side. A mounting plate (21) is fixedly provided on the side of the liquid chromatograph (1). A load-bearing block (30) is provided on the side of the mounting plate (21). A limit ring (31) is provided on the load-bearing block (30). A support shaft (32) is rotatably provided inside the limit ring (31). A power motor (33) is fixedly provided inside the load-bearing block (30). The output end of the power motor (33) is fixedly connected to the end of the support shaft (32). A fixing ring (34) is installed in a circumferential array on the support shaft (32). A storage cylinder (36) is fixedly provided inside the fixing ring (34). A delivery tube (37) is connected to the bottom of the storage cylinder (36). The storage cylinder (36) is equipped with a stirring mechanism.

2. The quantitative detection device for lactic acid bacteria metabolites according to claim 1, characterized in that: The connecting mechanism includes a load-bearing plate (11), a strip groove (12), a servo motor (13), a threaded rod (14), a sliding plate (15), and a connecting pipe (18). The load-bearing plate (11) is fixedly provided with a strip groove (12), and a servo motor (13) is fixedly provided at the end of the strip groove (12). The threaded rod (14) is rotatably provided inside the strip groove (12), and one end of the threaded rod (14) is fixedly connected to the output end of the servo motor (13). The sliding plate (15) passes through the strip groove (12), and the outer surface of the threaded rod (14) is threadedly connected to the inside of the sliding plate (15). The connecting pipe (18) is fixed inside the sliding plate (15), and one end of the connecting pipe (18) is fixedly connected to an infusion needle (19).

3. The quantitative detection device for lactic acid bacteria metabolites according to claim 2, characterized in that: The slide plate (15) is symmetrically provided with guide grooves (16), and the strip groove (12) is fixedly provided with guide blocks (17) corresponding to the guide grooves (16), and the guide blocks (17) are engaged with the corresponding guide grooves (16). The other end of the connecting pipe (18) is connected to a telescopic hose (20).

4. The quantitative detection device for lactic acid bacteria metabolites according to claim 3, characterized in that: The liquid chromatograph (1) is fixedly provided with a storage tank (7), an adjustment plate (8) is provided inside the storage tank (7), a cavity (9) is provided inside the adjustment plate (8), an electric push rod (10) is fixedly provided inside the storage tank (7), and the telescopic end of the electric push rod (10) is fixedly connected to the inner wall of the cavity (9).

5. The quantitative detection device for lactic acid bacteria metabolites according to claim 4, characterized in that: A liquid storage tank (22) is fixedly installed inside the mounting plate (21). A liquid addition pipe (23) is installed on the liquid storage tank (22). A pump (24) is fixedly installed inside the liquid storage tank (22). The input end of the pump (24) extends into the liquid storage tank (22), and the output end of the pump (24) is connected to a connecting pipe (25).

6. The quantitative detection device for lactic acid bacteria metabolites according to claim 5, characterized in that: The liquid chromatograph (1) has a plate (26) fixedly installed on its side. A conical groove (27) is fixedly installed on the plate (26). A delivery pump (28) is fixedly installed inside the plate (26). The input end of the delivery pump (28) is connected to the conical groove (27). The output end of the delivery pump (28) is fixedly connected to the other end of the telescopic hose (20).

7. The quantitative detection device for lactic acid bacteria metabolites according to claim 6, characterized in that: The conical groove (27) is inlaid with a diversion groove (29), and the other end of the connecting pipe (25) is connected to the diversion groove (29). Spray holes are arranged in an array on the diversion groove (29).

8. The quantitative detection device for lactic acid bacteria metabolites according to claim 1, characterized in that: A special-shaped frame (35) is fixedly installed on the side of the mounting plate (21), and a sealing cap (38) is threadedly connected to the top of the storage cylinder (36). A liquid filling head (39) is fixedly installed on the sealing cap (38).

9. The quantitative detection device for lactic acid bacteria metabolites according to claim 8, characterized in that: The stirring mechanism includes a rotating shaft (40), a stirring blade (41), and a driven gear (42). The rotating shaft (40) is rotatably disposed inside the sealing cover (38). The stirring blade (41) is fixed on the rotating shaft (40). One end of the rotating shaft (40) is fixedly connected to the center position of the driven gear (42). A gear ring (43) is fixedly disposed inside the special frame (35), and the gear ring (43) meshes with the driven gear (42).

10. A detection method for a quantitative detection device for lactic acid bacteria metabolites, applicable to the quantitative detection device for lactic acid bacteria metabolites as described in any one of claims 1-9, characterized in that, Includes the following steps: S1. Start the liquid chromatograph (1) and preheat it. Pour in the mobile phase to continuously flush the column until the instrument baseline remains stable. S2. Then inject blank samples for detection to confirm that there are no interfering peaks in the spectrum. In order to reduce the detection error caused by instrument signal drift, strictly follow the order of standard, test sample and standard to inject in sequence. Repeat the injection of each sample 1-2 times through the injector (5) and take the average value of the detection to effectively improve the repeatability of the results. S3, Liquid Chromatography Analyzer (1) Automatically collects and records the retention time and peak area of ​​each group of samples, compares the retention time of the sample to be tested with that of the standard, determines the components of the metabolites, and finally realizes the quantitative detection of lactic acid bacteria metabolites.

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