A method for artificial propagation of an oriental paddlefish

Abstract

The application discloses an artificial breeding method of oriental blackspot fish, comprising the following steps: S1, parent fish selection; S2, parturition injection; S3, insemination; S4, fertilized egg hatching; and S5, fry cultivation. The artificial breeding method of the oriental blackspot fish provided by the application aims at the technical problems of the oriental blackspot fish, such as inconsistent response of parent fish to parturition, low insemination efficiency caused by large amount of sperm, and harsh hatching conditions of drifting eggs, and the like, and the method comprises the following steps: establishing a differentiated parturition scheme for female and male, a special sperm diluent formula, a linkage quantitative insemination system, an accurate constant-temperature high-dissolved-oxygen hatching process, forming a complete artificial breeding technical system covering parent fish selection to fry cultivation, and realizing the standardization and repeatable operation of each key link of the artificial breeding of the oriental blackspot fish, thereby providing systematic technical support for the large-scale fry production of the species.

Description

Technical Field

[0001] This invention belongs to the field of fish seedling breeding technology, specifically relating to an artificial breeding method for the Oriental blackfish. Background Technology

[0002] With the impact of human activities such as the development of cascade hydropower in the Pearl River Basin, river dredging and renovation, water pollution, and human fishing operations, the ecological environment of the natural water system in the south has been continuously changed. The original habitats of fish have been fragmented, and the physical and chemical indicators of water bodies and water flow conditions have gradually deviated from the natural original standards. The habitat space on which native fish depend for survival has been continuously reduced.

[0003] Oriental cuttlefish ( Garra orientalis The Oriental Blackfish (Siniperca spp.), belonging to the order Cypriniformes, family Cyprinidae, subfamily Scutiginae, and genus Scutigera, is widely distributed in various water systems of southern my country. It is a native freshwater fish unique to southern my country, primarily inhabiting fast-flowing streams, shallow riverbanks, and rocky crevices with gravelly bottoms. It is not only an indispensable component of the aquatic ecosystem of its basin, effectively reflecting the health of the aquatic environment, but also possesses ornamental value and local aquatic product utilization value. Due to the combined effects of significant changes in hydrological rhythms, the disappearance of natural spawning grounds, and the deterioration of its habitat, the wild population of Oriental Blackfish has been shrinking year by year, with wild resources continuing to decline, posing a severe challenge to its reproduction and development.

[0004] At present, the artificial breeding technology system for freshwater economic fish, ornamental fish and common native fish has been developed relatively well. The industry-standard fish breeding process mainly covers key steps such as intensive breeding of parent fish, exogenous hormone induction of spawning, artificial insemination, incubation of fish eggs in flowing or still water, and normalized domestication and breeding of larvae. This mature technology has been widely used in the breeding and production of most freshwater fish such as Cyprinidae and Barbinae.

[0005] Domestic and international research on the genus *Hemiberlesia* and related stream-native fishes has largely focused on species classification, natural distribution surveys, morphological identification, wild resource inventory verification, and basic physiological and ecological analysis. Only a few studies document basic aquaculture attempts and breeding of *Hemiberlesia*. Related research generally follows the common breeding models for conventional benthic fishes, with a preference for broadly adaptable species. There is currently no systematic research or practical plan specifically for artificial breeding techniques of *Hemiberlesia orientalis*.

[0006] The Oriental blackfish possesses unique biological characteristics, including high habitat requirements, strong aquatic stress response, and weak embryonic tolerance to changes in the external environment. Furthermore, its breeding stage has stringent requirements regarding water flow velocity, temperature, and quality. Directly applying existing conventional fish breeding techniques cannot meet its reproductive physiological needs and makes it difficult to achieve stable seedling production. Therefore, it is urgent to develop artificial breeding techniques specifically adapted to the Oriental blackfish, taking into account its unique characteristics. Summary of the Invention

[0007] In view of the shortcomings of the existing technology, the purpose of this invention is to provide an artificial breeding method for the Oriental blackfish.

[0008] To achieve the above objectives, the present invention provides the following technical solution: An artificial breeding method for the Oriental squid includes the following steps: S1. Selection of parent fish: During the natural breeding season, select female and male parent fish that have mature gonads and are healthy and undamaged. S2. Induced Spawning Injection: A differentiated injection method is adopted for males and females. The second injection is given 12 hours after the first injection. The male fish is injected at the same time as the female fish for the second injection. After the injection, the male and female parent fish are transferred to a closed, light-proof tank at a 1:1 ratio to wait for spawning. The tank is continuously aerated to maintain a high dissolved oxygen environment and a constant temperature of 20 ℃. Starting from 10 hours after the second injection, the tank is opened every 2 hours to observe the spawning situation. After the eggs are detected and the spawning is found, the eggs are removed and transferred to the hatching tank. After the water is changed, the fish continue to wait for spawning in the original tank until the spawning is completed. S3. Insemination: Collect semen, add a special diluent, then mix with eggs, add filtered clean water, stir thoroughly and rinse repeatedly until the liquid is clear to obtain fertilized eggs; S4. Incubation of fertilized eggs: Transfer the fertilized eggs into an incubation tank with a filter and incubate them using a micro-flow water system. S5. Fry rearing: After the fry break through the membrane, transfer them to a rearing tank, feed them according to the stages and gradually acclimate them to their diet.

[0009] Preferably, in step S1, the female parent fish are selected from healthy individuals with a weight of over 50 g, a swollen and soft abdomen, and a slightly protruding genital opening; the male parent fish are selected from robust individuals with a weight of over 20 g, a slender body, a firm and non-swollen abdomen, and a milky white semen flowing out when the abdomen is gently pressed.

[0010] Preferably, in step S2, the formulation dosage of the first injection is 23 μg / kg of luteinizing hormone-releasing hormone analog LRH-A + 2 mg / kg of dioxonone maleate DOM, the formulation dosage of the second injection is 20 μg / kg of luteinizing hormone-releasing hormone analog LRH-A2 + 10 mg / kg of dioxonone maleate DOM + 400 IU / kg of human chorionic gonadotropin (HCG), and the formulation dosage of a single injection is 10 μg / kg of luteinizing hormone-releasing hormone analog LRH-A2 + 5 mg / kg of dioxonone maleate DOM + 200 IU / kg of human chorionic gonadotropin (HCG); the injection method is an oblique injection technique at the base of the pectoral fin, and the injection drug is dissolved in physiological saline to prepare an injection solution, with an injection solution volume of 4 mL / kg.

[0011] Preferably, in step S2, 24 hours after the second injection, a third injection is administered to female fish that have not yet laid eggs to induce spawning. The formulation is 220 μg / kg of luteinizing hormone-releasing hormone analog LRH-A, 10 mg / kg of dioxin maleate (DOM), and 2000 IU / kg of human chorionic gonadotropin (HCG). Simultaneously, male fish are given a second injection with the formulation of 10 μg / kg of luteinizing hormone-releasing hormone analog LRH-A, 5 mg / kg of dioxin maleate (DOM), and 200 IU / kg of human chorionic gonadotropin (HCG). The injection method and dosage are the same as the first and second injections. Starting 6 hours after the third injection, spawning is checked every 2 hours. 24 hours after the third injection, artificial egg collection is performed on female fish that have not yet laid eggs.

[0012] The spawning of Oriental blackfish under induced spawning conditions is characterized by dispersed batches and inconsistent individual response times. This invention employs a dual breeding model that prioritizes natural spawning while supplementing it with artificial egg collection. During the spawning phase, naturally spawned eggs are collected in batches through regular inspections to prevent eggs from remaining in the tank for extended periods, which could lead to decreased egg quality or missed fertilization opportunities. For individuals that still cannot spawn naturally after a third dose of induced spawning, artificial egg collection is promptly performed, and the eggs are inseminated according to standard procedures, ensuring full utilization of parent fish reproductive resources. This model is designed specifically for the dispersed spawning rhythm of Oriental blackfish, maximizing the effective egg recovery rate per batch while ensuring egg quality.

[0013] Preferably, in step S3, the volume ratio of semen to special diluent is 1:8-12, the amount of filtered clean water added is 4-6 times the volume of the sperm-egg mixture, the stirring time is 2-10 min, and the rinsing is 3-4 times.

[0014] Preferably, the special diluent in step S3 has the following composition: NaCl 5.8-6.5 g / L, KCl 0.15-0.3 g / L, CaCl2 0.1-0.2 g / L, glucose 0.9-1.8 g / L, NaHCO3 0.4-0.8 g / L, reduced glutathione 0.15-0.3 g / L, arginine 0.05-0.09 g / L, inositol 0.2-0.5 g / L, and the balance being distilled water.

[0015] Preferably, the special diluent in step S3 has the following composition: NaCl 6.3 g / L, KCl 0.25 g / L, CaCl2 0.15 g / L, glucose 1.4 g / L, NaHCO3 0.6 g / L, reduced glutathione 0.25 g / L, arginine 0.07 g / L, inositol 0.4 g / L, and the balance being distilled water.

[0016] Preferably, the incubation temperature in step S4 is maintained at a constant 20 ℃, with a temperature difference fluctuation not exceeding ±0.5 ℃, and the incubation time is 40-42 h.

[0017] This invention proposes for the first time that the eggs of the Oriental blackfish are drifting. In response to the drifting nature of these eggs, a micro-flow incubation mode with a filter-equipped incubation tank is specifically adopted. The incubation water undergoes pre-temperature control treatment before entering the incubation tank to ensure that the incoming water temperature is consistent with the temperature inside the tank. Oriental blackfish embryos are extremely sensitive to water temperature; the hatching rate decreases significantly when the water temperature is above 20 ℃, and embryonic development is almost impossible when the water temperature exceeds 24 ℃. The pre-temperature control design specifically provides precise temperature protection for the above-mentioned species specificities.

[0018] The fertilization process was conducted entirely at a constant temperature of 20°C. Water temperature control has a dual significance for fertilization efficiency. On the one hand, at 20°C, sperm motility and survival time are within an optimal range; excessively high temperatures accelerate sperm energy consumption, shorten effective motility time, and directly reduce the probability of egg fertilization. On the other hand, eggs are equally sensitive to environmental temperature after being removed from the body; maintaining a stable low-temperature environment helps slow down egg viability decline and prolongs the effective fertilization window. Both the fertilization and hatching stages were conducted at a uniform 20°C temperature, ensuring consistent temperature management throughout the entire process from gamete activation to embryonic development, which aligns with the habitat characteristics of the native low-temperature, fast-flowing waters of the Oriental blackfish.

[0019] Preferably, the hatching tank in step S4 is also equipped with a microporous aeration device, which maintains the dissolved oxygen in the hatching water at 7-9 mg / L through dual oxygenation by micro-flow water supplementation and microporous aeration. In this invention, the upward flow of bubbles generated by micropore aeration works synergistically with the micro-flow to maintain the drifting eggs uniformly suspended in the middle layer of the water under moderate flow conditions, thus avoiding the risk of mechanical damage to the egg membrane caused by simply relying on high flow velocity for buoyancy.

[0020] The Oriental blackfish naturally inhabits fast-flowing, gravelly bottom waters, and the hatching of its drifting eggs requires higher dissolved oxygen levels than that of fish in still or slow-flowing waters. This invention utilizes a dual oxygenation approach—micro-flow aeration and micro-pore aeration—to maintain dissolved oxygen in the hatching water at 7-9 mg / L, simulating its natural, high-oxygen-content natural fast-flowing hatching environment and meeting the oxygen consumption requirements of embryonic development. Simultaneously, the upward flow of fine bubbles generated by micro-pore aeration, working synergistically with the micro-flow, ensures uniform suspension of the drifting eggs at lower flow velocities, avoiding mechanical damage to the egg membrane caused by relying solely on high-velocity buoyancy. This ensures sufficient oxygen supply to the eggs while reducing the egg membrane breakage rate.

[0021] Preferably, the specific process of fish fry cultivation in step S5 is as follows: Days 0-3 after hatching: The fry rely on their own yolk sac for nutrition and do not need to be fed. They can be left to rest and develop. 4-7 days after membrane rupture: Feed with brine shrimp; Day 8 after hatching: Remove the fry from the rearing tank and put them into the pond. Add crushed compound feed to the brine shrimp in proportion, gradually reduce the proportion of brine shrimp to acclimate their feeding habits. Stop feeding brine shrimp on day 12 and let the fry eat only the artificial compound feed. Day 13 after membrane rupture: Feed the animal twice daily, at 9:00 AM and 7:00 PM, with finely chopped feed. Water quality management: Change 1 / 3 to 1 / 2 of the pool water every 6-8 days to ensure a stable aquatic environment for growth; Nutritional fortification: Add 10% compound algae powder to the compound feed once a week.

[0022] Compared with the prior art, the present invention has the following beneficial effects: (1) The artificial breeding method for Oriental blackfish provided by this invention addresses the species-specific technical challenges in the artificial breeding of Oriental blackfish, such as inconsistent spawning response of parent fish, low fertilization efficiency due to excessive semen volume, and harsh hatching conditions for drifting eggs. By establishing a sex-differentiated spawning induction scheme, a special semen dilution solution formula and a linked quantitative fertilization system, and a precise constant temperature and high dissolved oxygen hatching process, a complete artificial breeding technology system covering parent fish selection to fry cultivation is formed. This achieves standardization and repeatability of each key link in the artificial breeding of Oriental blackfish, providing systematic technical support for the large-scale fry production of this species.

[0023] (2) The artificial breeding method for Oriental blackfish provided by the present invention addresses the physiological characteristics of significant differences in the gonadal development rhythm and inconsistent spawning response time between male and female Oriental blackfish. It adopts a differentiated spawning induction scheme with two separate injections for female fish and a single injection for male fish, which is synchronized with the second injection. This allows the gonadal development of male and female fish to be synchronized during the fertilization operation window, reducing the risk of over-mature eggs or premature sperm inactivation caused by asynchrony between male and female fish. At the same time, for cases where spawning is delayed in some individuals, a third injection remedial spawning induction procedure is set up and a timed inspection mechanism is used to effectively reduce the waste of parent fish resources caused by spawning failure and improve the overall spawning rate of a single batch of spawning.

[0024] (3) The artificial breeding method for Oriental squid provided by this invention has a relatively large sperm volume. If it is directly mixed with eggs, the sperm will contain a high concentration of potassium. + It can prematurely activate sperm, causing them to exhaust their motility before contacting the egg, thus reducing the fertilization rate. This invention uses NaCl as the main osmotic pressure regulator, combined with KCl and CaCl2 to maintain ion balance, and NaHCO3 to adjust the pH to 7.0-7.5. Glucose provides the energy substrate. Furthermore, reduced glutathione is introduced to remove reactive oxygen species generated during dilution, reducing sperm oxidative damage. Inositol is introduced to help maintain sperm cell osmotic stress tolerance, and arginine is introduced to provide additional nitrogen and energy support for sperm motility. These components work synergistically in the dilution protection scenario before semen activation, helping to maintain sperm motility during the fertilization window and improving the effective fertilization rate. Considering the relatively large semen volume of the Oriental blackfish, the volume ratio of semen to diluent is determined to be 1:8-12. This system, on the one hand, inhibits premature sperm activation through sufficient dilution, and on the other hand, ensures effective sperm density around the egg by increasing the male ratio, establishing a balance between dilution protection and fertilization efficiency. This avoids the problems of insufficient sperm density due to simply increasing the dilution ratio or wasteful operation due to relying solely on a high sperm-to-egg ratio. Detailed Implementation

[0025] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to specific examples. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0026] Unless otherwise specified, all chemical reagents and materials in this invention are purchased from the market or synthesized from raw materials purchased from the market.

[0027] Example 1 An artificial breeding method for the Oriental squid includes the following steps: S1. Selection of broodstock: During the natural breeding season, select female and male broodstock that are mature in gonads, robust and undamaged. The female broodstock should weigh over 50 g, have a soft, swollen abdomen, and a slightly protruding genital opening. The male broodstock should weigh over 20 g, have a slender body, a firm, non-swollen abdomen, and be robust individuals that produce milky white semen when the abdomen is gently pressed. S2. Induced Spawning Injection: A differentiated, fractionated injection method is used, with the second injection administered 12 hours after the first. The dosage for the first injection is 23 μg / kg of luteinizing hormone-releasing hormone analogue (LRH-A) + 2 mg / kg of dioxin maleate (DOM). The dosage for the second injection is 20 μg / kg of LRH-A + 10 mg / kg of dioxin maleate (DOM) + 400 IU / kg of human chorionic gonadotropin (HCG). Male fish receive a single injection simultaneously with the second injection for females, with a dosage of 10 μg / kg of LRH-A + 5 mg / kg of dioxin maleate (DOM) + 200 IU / kg of HCG. The injection method is an oblique injection at the base of the pectoral fin. The injection solution is prepared by dissolving the drugs in physiological saline, and the volume of the injection solution is 4... mL / kg; After injection, male and female parent fish were transferred to a closed, opaque tank at a 1:1 ratio to await spawning. A high dissolved oxygen environment was maintained continuously at a constant temperature of 20 ℃. Starting 10 hours after the second injection, the tank was opened every 2 hours to observe spawning. Once eggs were detected and spawning was observed, the eggs were removed and transferred to an incubation tank. After water changes, the fish continued to spawn in the original tank until spawning was complete. 24 hours after the second injection, a third injection was administered to female fish that had not yet spawned to induce spawning. The formula was: luteinizing hormone-releasing hormone analog LRH-A 220 μg / kg + dioxin maleate DOM 10 mg / kg + human chorionic gonadotropin (HCG) 2000 IU / kg. Simultaneously, male fish were given a second injection: luteinizing hormone-releasing hormone analog LRH-A 210 μg / kg + dioxin maleate DOM 5 mg / kg + human chorionic gonadotropin (HCG) 2000 IU / kg. IU / kg; the injection method and dosage are the same as the first and second injections; starting 6 hours after the third injection, check the spawning situation every 2 hours; 24 hours after the third injection, perform artificial spawning on female fish that have not yet spawned; S3. Insemination: Collect semen and add a special diluent at a volume ratio of 1:10. Then mix with eggs, adding 0.8 mL of diluted semen per gram of eggs. Next, add filtered clean water at a volume 5 times that of the sperm-egg mixture. Stir thoroughly and rinse repeatedly until the liquid is clear. Stir for 6 minutes and rinse 4 times to obtain fertilized eggs. S4. Fertilized egg incubation: The fertilized eggs are transferred to an incubation tank with a filter screen and incubated using micro-flow water. The incubation tank is also equipped with a microporous aeration device. Through the dual oxygenation of micro-flow water supplementation and microporous aeration, the dissolved oxygen in the incubation water is maintained at 7-9 mg / L. The incubation temperature is maintained at a constant 20 ℃, with a temperature difference fluctuation not exceeding ±0.5 ℃. The incubation time is 41 h. S5. Fry Rearing: After hatching, the fry are transferred to rearing tanks. Days 0-3 after hatching: The fry rely on their own yolk sac for nutrition and do not require feeding; allow them to rest. Days 4-7 after hatching: Feed with brine shrimp. Day 8 after hatching: Remove from the rearing tanks and release into the pond. Gradually add crushed formulated feed to the brine shrimp, gradually reducing the proportion of brine shrimp to acclimate their feeding habits. Stop feeding brine shrimp on day 12, allowing the fry to fully consume formulated feed. Day 13 after hatching: Feed with finely crushed feed twice daily, at 9:00 AM and 7:00 PM. Water Quality Management: Change 1 / 3-1 / 2 of the pond water every 6-8 days to ensure a stable aquatic environment for growth. Nutritional Fortification: Add 10% compound algae powder to the formulated feed once a week.

[0028] The special diluent mentioned in step S3 has the following composition: NaCl 6.3 g / L, KCl 0.25 g / L, CaCl2 0.15 g / L, glucose 1.4 g / L, NaHCO3 0.6 g / L, reduced glutathione 0.25 g / L, arginine 0.07 g / L, inositol 0.4 g / L, and the balance is distilled water.

[0029] In this embodiment, the fertilization rate of sperm and egg was 85.6%, the hatching rate was 94.1%, and the survival rate of fish fry after 30 days of cultivation was 91.7%. The overall breeding effect was excellent and large-scale breeding can be stably achieved.

[0030] Example 2 An artificial breeding method for the Oriental squid includes the following steps: S1. Selection of broodstock: During the natural breeding season, select female and male broodstock that are mature in gonads, robust and undamaged. The female broodstock should weigh over 50 g, have a soft, swollen abdomen, and a slightly protruding genital opening. The male broodstock should weigh over 20 g, have a slender body, a firm, non-swollen abdomen, and be robust individuals that produce milky white semen when the abdomen is gently pressed. S2. Induced Spawning Injection: A differentiated, fractionated injection method is used, with the second injection administered 12 hours after the first. The dosage for the first injection is 23 μg / kg of luteinizing hormone-releasing hormone analogue (LRH-A) + 2 mg / kg of dioxin maleate (DOM). The dosage for the second injection is 20 μg / kg of LRH-A + 10 mg / kg of dioxin maleate (DOM) + 400 IU / kg of human chorionic gonadotropin (HCG). Male fish receive a single injection simultaneously with the second injection for females, with a dosage of 10 μg / kg of LRH-A + 5 mg / kg of dioxin maleate (DOM) + 200 IU / kg of HCG. The injection method is an oblique injection at the base of the pectoral fin. The injection solution is prepared by dissolving the drugs in physiological saline, and the volume of the injection solution is 4... mL / kg; After injection, male and female parent fish were transferred to a closed, opaque tank at a 1:1 ratio to await spawning. A high dissolved oxygen environment was maintained continuously at a constant temperature of 20 ℃. Starting 10 hours after the second injection, the tank was opened every 2 hours to observe spawning. Once eggs were detected and spawning was observed, the eggs were removed and transferred to an incubation tank. After water changes, the fish continued to spawn in the original tank until spawning was complete. 24 hours after the second injection, a third injection was administered to female fish that had not yet spawned to induce spawning. The formula was: luteinizing hormone-releasing hormone analog LRH-A 220 μg / kg + dioxin maleate DOM 10 mg / kg + human chorionic gonadotropin (HCG) 2000 IU / kg. Simultaneously, male fish were given a second injection: luteinizing hormone-releasing hormone analog LRH-A 210 μg / kg + dioxin maleate DOM 5 mg / kg + human chorionic gonadotropin (HCG) 2000 IU / kg. IU / kg; the injection method and dosage are the same as the first and second injections; starting 6 hours after the third injection, check the spawning situation every 2 hours; 24 hours after the third injection, perform artificial spawning on female fish that have not yet spawned; S3. Insemination: Collect semen and add a special diluent at a volume ratio of 1:8. Then mix with eggs, adding 0.6 mL of diluted semen per gram of eggs. Next, add filtered clean water at a volume 4 times that of the sperm-egg mixture. Stir thoroughly and rinse repeatedly until the liquid is clear. Stir for 2 minutes and rinse 3 times to obtain fertilized eggs. S4. Fertilized egg incubation: The fertilized eggs are transferred to an incubation tank with a filter screen and incubated using micro-flow water. The incubation tank is also equipped with a microporous aeration device. Through the dual oxygenation of micro-flow water supplementation and microporous aeration, the dissolved oxygen in the incubation water is maintained at 7-9 mg / L. The incubation temperature is kept constant at 20 ℃, with a temperature difference fluctuation not exceeding ±0.5 ℃. The incubation time is 40 h. S5. Fry Rearing: After hatching, the fry are transferred to rearing tanks. Days 0-3 after hatching: The fry rely on their own yolk sac for nutrition and do not require feeding; allow them to rest. Days 4-7 after hatching: Feed with brine shrimp. Day 8 after hatching: Remove from the rearing tanks and release into the pond. Gradually add crushed formulated feed to the brine shrimp, gradually reducing the proportion of brine shrimp to acclimate their feeding habits. Stop feeding brine shrimp on day 12, allowing the fry to fully consume formulated feed. Day 13 after hatching: Feed with finely crushed feed twice daily, at 9:00 AM and 7:00 PM. Water Quality Management: Change 1 / 3-1 / 2 of the pond water every 6-8 days to ensure a stable aquatic environment for growth. Nutritional Fortification: Add 10% compound algae powder to the formulated feed once a week.

[0031] The special diluent mentioned in step S3 has the following composition: NaCl 5.8 g / L, KCl 0.15 g / L, CaCl2 0.1 g / L, glucose 0.9 g / L, NaHCO3 0.4 g / L, reduced glutathione 0.15 g / L, arginine 0.05 g / L, inositol 0.2 g / L, and the balance is distilled water.

[0032] In this embodiment, the fertilization rate of sperm and egg was 84.9%, the hatching rate was 91.7%, and the survival rate of fish fry after 30 days of cultivation was 88.5%. The overall breeding effect was excellent and large-scale breeding can be stably achieved.

[0033] Example 3 An artificial breeding method for the Oriental squid includes the following steps: S1. Selection of broodstock: During the natural breeding season, select female and male broodstock that are mature in gonads, robust and undamaged. The female broodstock should weigh over 50 g, have a soft, swollen abdomen, and a slightly protruding genital opening. The male broodstock should weigh over 20 g, have a slender body, a firm, non-swollen abdomen, and be robust individuals that produce milky white semen when the abdomen is gently pressed. S2. Induced Spawning Injection: A differentiated, fractionated injection method is used, with the second injection administered 12 hours after the first. The dosage for the first injection is 23 μg / kg of luteinizing hormone-releasing hormone analogue (LRH-A) + 2 mg / kg of dioxin maleate (DOM). The dosage for the second injection is 20 μg / kg of LRH-A + 10 mg / kg of dioxin maleate (DOM) + 400 IU / kg of human chorionic gonadotropin (HCG). Male fish receive a single injection simultaneously with the second injection for females, with a dosage of 10 μg / kg of LRH-A + 5 mg / kg of dioxin maleate (DOM) + 200 IU / kg of HCG. The injection method is an oblique injection at the base of the pectoral fin. The injection solution is prepared by dissolving the drugs in physiological saline, and the volume of the injection solution is 4... mL / kg; After injection, male and female parent fish were transferred to a closed, opaque tank at a 1:1 ratio to await spawning. A high dissolved oxygen environment was maintained continuously at a constant temperature of 20 ℃. Starting 10 hours after the second injection, the tank was opened every 2 hours to observe spawning. Once eggs were detected and spawning was observed, the eggs were removed and transferred to an incubation tank. After water changes, the fish continued to spawn in the original tank until spawning was complete. 24 hours after the second injection, a third injection was administered to female fish that had not yet spawned to induce spawning. The formula was: luteinizing hormone-releasing hormone analog LRH-A 220 μg / kg + dioxin maleate DOM 10 mg / kg + human chorionic gonadotropin (HCG) 2000 IU / kg. Simultaneously, male fish were given a second injection: luteinizing hormone-releasing hormone analog LRH-A 210 μg / kg + dioxin maleate DOM 5 mg / kg + human chorionic gonadotropin (HCG) 2000 IU / kg. IU / kg; the injection method and dosage are the same as the first and second injections; starting 6 hours after the third injection, check the spawning situation every 2 hours; 24 hours after the third injection, perform artificial spawning on female fish that have not yet spawned; S3. Insemination: Collect semen and add a special diluent at a volume ratio of 1:12. Then mix with eggs, adding 1 mL of diluted semen per gram of eggs. Next, add filtered clean water at a volume 6 times that of the sperm-egg mixture. Stir thoroughly and rinse repeatedly until the liquid is clear. Stir for 10 minutes and rinse 4 times to obtain fertilized eggs. S4. Fertilized egg incubation: The fertilized eggs are transferred to an incubation tank with a filter screen and incubated using micro-flow water. The incubation tank is also equipped with a microporous aeration device. Through the dual oxygenation of micro-flow water supplementation and microporous aeration, the dissolved oxygen in the incubation water is maintained at 7-9 mg / L. The incubation temperature is maintained at a constant 20 ℃, with a temperature difference fluctuation not exceeding ±0.5 ℃. The incubation time is 42 h. S5. Fry Rearing: After hatching, the fry are transferred to rearing tanks. Days 0-3 after hatching: The fry rely on their own yolk sac for nutrition and do not require feeding; allow them to rest. Days 4-7 after hatching: Feed with brine shrimp. Day 8 after hatching: Remove from the rearing tanks and release into the pond. Gradually add crushed formulated feed to the brine shrimp, gradually reducing the proportion of brine shrimp to acclimate their feeding habits. Stop feeding brine shrimp on day 12, allowing the fry to fully consume formulated feed. Day 13 after hatching: Feed with finely crushed feed twice daily, at 9:00 AM and 7:00 PM. Water Quality Management: Change 1 / 3-1 / 2 of the pond water every 6-8 days to ensure a stable aquatic environment for growth. Nutritional Fortification: Add 10% compound algae powder to the formulated feed once a week.

[0034] The special diluent mentioned in step S3 has the following composition: NaCl 6.5 g / L, KCl 0.3 g / L, CaCl2 0.2 g / L, glucose 1.8 g / L, NaHCO3 0.8 g / L, reduced glutathione 0.3 g / L, arginine 0.09 g / L, inositol 0.5 g / L, and the balance is distilled water.

[0035] In this embodiment, the fertilization rate of sperm and egg was 85.4%, the hatching rate was 93.2%, and the survival rate of fish fry after 30 days of cultivation was 90.7%. The overall breeding effect was excellent and large-scale breeding can be stably achieved.

[0036] Comparative Example 1 An artificial breeding method for the Oriental squid includes the following steps: S1. Selection of broodstock: During the natural breeding season, select female and male broodstock that are mature in gonads, robust and undamaged. The female broodstock should weigh over 50 g, have a soft, swollen abdomen, and a slightly protruding genital opening. The male broodstock should weigh over 20 g, have a slender body, a firm, non-swollen abdomen, and be robust individuals that produce milky white semen when the abdomen is gently pressed. S2. Induced Spawning Injection: A differentiated, fractionated injection method is used, with the second injection administered 12 hours after the first. The dosage for the first injection is 23 μg / kg of luteinizing hormone-releasing hormone analogue (LRH-A) + 2 mg / kg of dioxin maleate (DOM). The dosage for the second injection is 20 μg / kg of LRH-A + 10 mg / kg of dioxin maleate (DOM) + 400 IU / kg of human chorionic gonadotropin (HCG). Male fish receive a single injection simultaneously with the second injection for females, with a dosage of 10 μg / kg of LRH-A + 5 mg / kg of dioxin maleate (DOM) + 200 IU / kg of HCG. The injection method is an oblique injection at the base of the pectoral fin. The injection solution is prepared by dissolving the drugs in physiological saline, and the volume of the injection solution is 4... mL / kg; After injection, male and female parent fish were transferred to a closed, light-proof tank at a 1:1 ratio to await spawning. A high dissolved oxygen environment was maintained continuously at a constant temperature of 28 ℃. Starting 10 hours after the second injection, the tank was opened every 2 hours to observe spawning. Once eggs were detected and spawning was observed, the eggs were removed and transferred to an incubation tank. After water changes, the fish continued to spawn in the original tank until spawning was complete. 24 hours after the second injection, a third injection was administered to female fish that had not yet spawned to induce spawning. The formula was 220 μg / kg of luteinizing hormone-releasing hormone analog LRH-A + 10 mg / kg of dioxin maleate (DOM) + 2000 IU / kg of human chorionic gonadotropin (HCG). Simultaneously, male fish were given a second injection with the formula: 10 μg / kg of luteinizing hormone-releasing hormone analog LRH-A + 5 mg / kg of dioxin maleate (DOM) + 200 IU / kg of human chorionic gonadotropin (HCG). IU / kg; the injection method and dosage were the same as the first and second injections; starting 6 hours after the third injection, the spawning situation was checked every 2 hours; if the female fish's eggs dissolved in the abdominal cavity but did not spawn naturally, the experiment was declared a failure.

[0037] Compared to Example 1, the temperature of the broodstock spawning chamber in this comparative example was 28 °C.

[0038] Comparative Example 2 An artificial breeding method for the Oriental squid includes the following steps: S1. Selection of broodstock: During the natural breeding season, select female and male broodstock that are mature in gonads, robust and undamaged. The female broodstock should weigh over 50 g, have a soft, swollen abdomen, and a slightly protruding genital opening. The male broodstock should weigh over 20 g, have a slender body, a firm, non-swollen abdomen, and be robust individuals that produce milky white semen when the abdomen is gently pressed. S2. Induced Spawning Injection: A differentiated, fractionated injection method is used, with the second injection administered 12 hours after the first. The dosage for the first injection is 23 μg / kg of luteinizing hormone-releasing hormone analogue (LRH-A) + 2 mg / kg of dioxin maleate (DOM). The dosage for the second injection is 20 μg / kg of LRH-A + 10 mg / kg of dioxin maleate (DOM) + 400 IU / kg of human chorionic gonadotropin (HCG). Male fish receive a single injection simultaneously with the second injection for females, with a dosage of 10 μg / kg of LRH-A + 5 mg / kg of dioxin maleate (DOM) + 200 IU / kg of HCG. The injection method is an oblique injection at the base of the pectoral fin. The injection solution is prepared by dissolving the drugs in physiological saline, and the volume of the injection solution is 4... mL / kg; After injection, male and female parent fish were transferred to a closed, opaque tank at a 1:1 ratio to await spawning. A high dissolved oxygen environment was maintained continuously at a constant temperature of 20 ℃. Starting 10 hours after the second injection, the tank was opened every 2 hours to observe spawning. Once eggs were detected and spawning was observed, the eggs were removed and transferred to an incubation tank. After water changes, the fish continued to spawn in the original tank until spawning was complete. 24 hours after the second injection, a third injection was administered to female fish that had not yet spawned to induce spawning. The formula was: luteinizing hormone-releasing hormone analog LRH-A 220 μg / kg + dioxin maleate DOM 10 mg / kg + human chorionic gonadotropin (HCG) 2000 IU / kg. Simultaneously, male fish were given a second injection: luteinizing hormone-releasing hormone analog LRH-A 210 μg / kg + dioxin maleate DOM 5 mg / kg + human chorionic gonadotropin (HCG) 2000 IU / kg. IU / kg; the injection method and dosage are the same as the first and second injections; starting 6 hours after the third injection, check the spawning situation every 2 hours; 24 hours after the third injection, perform artificial spawning on female fish that have not yet spawned; S3. Insemination: Collect semen and mix it with eggs. Add 0.08 mL of semen per gram of eggs, then add filtered clean water. The amount of filtered clean water added is 5 times the volume of the sperm-egg mixture. Stir thoroughly and rinse repeatedly until the liquid is clear. Stir for 6 minutes and rinse 4 times to obtain fertilized eggs. S4. Fertilized egg incubation: The fertilized eggs are transferred to an incubation tank with a filter screen and incubated using micro-flow water. The incubation tank is also equipped with a microporous aeration device. Through the dual oxygenation of micro-flow water supplementation and microporous aeration, the dissolved oxygen in the incubation water is maintained at 7-9 mg / L. The incubation temperature is kept constant at 20 ℃, with a temperature difference fluctuation not exceeding ±0.5 ℃. The incubation time is 40 h. S5. Fry Rearing: After hatching, the fry are transferred to rearing tanks. Days 0-3 after hatching: The fry rely on their own yolk sac for nutrition and do not require feeding; allow them to rest. Days 4-7 after hatching: Feed with brine shrimp. Day 8 after hatching: Remove from the rearing tanks and release into the pond. Gradually add crushed formulated feed to the brine shrimp, gradually reducing the proportion of brine shrimp to acclimate their feeding habits. Stop feeding brine shrimp on day 12, allowing the fry to fully consume formulated feed. Day 13 after hatching: Feed with finely crushed feed twice daily, at 9:00 AM and 7:00 PM. Water Quality Management: Change 1 / 3-1 / 2 of the pond water every 6-8 days to ensure a stable aquatic environment for growth. Nutritional Fortification: Add 10% compound algae powder to the formulated feed once a week.

[0039] In this example, the fertilization rate of sperm and egg was 51.3%, the hatching rate was 74.5%, and the survival rate of fish fry after 30 days of cultivation was 78.2%.

[0040] Compared to Example 1, the semen in this comparative example was not diluted.

[0041] Comparative Example 3 An artificial breeding method for the Oriental squid includes the following steps: S1. Selection of broodstock: During the natural breeding season, select female and male broodstock that are mature in gonads, robust and undamaged. The female broodstock should weigh over 50 g, have a soft, swollen abdomen, and a slightly protruding genital opening. The male broodstock should weigh over 20 g, have a slender body, a firm, non-swollen abdomen, and be robust individuals that produce milky white semen when the abdomen is gently pressed. S2. Induced Spawning Injection: A differentiated, fractionated injection method is used, with the second injection administered 12 hours after the first. The dosage for the first injection is 23 μg / kg of luteinizing hormone-releasing hormone analogue (LRH-A) + 2 mg / kg of dioxin maleate (DOM). The dosage for the second injection is 20 μg / kg of LRH-A + 10 mg / kg of dioxin maleate (DOM) + 400 IU / kg of human chorionic gonadotropin (HCG). Male fish receive a single injection simultaneously with the second injection for females, with a dosage of 10 μg / kg of LRH-A + 5 mg / kg of dioxin maleate (DOM) + 200 IU / kg of HCG. The injection method is an oblique injection at the base of the pectoral fin. The injection solution is prepared by dissolving the drugs in physiological saline, and the volume of the injection solution is 4... mL / kg; After injection, male and female parent fish were transferred to a closed, opaque tank at a 1:1 ratio to await spawning. A high dissolved oxygen environment was maintained continuously at a constant temperature of 20 ℃. Starting 10 hours after the second injection, the tank was opened every 2 hours to observe spawning. Once eggs were detected and spawning was observed, the eggs were removed and transferred to an incubation tank. After water changes, the fish continued to spawn in the original tank until spawning was complete. 24 hours after the second injection, a third injection was administered to female fish that had not yet spawned to induce spawning. The formula was: luteinizing hormone-releasing hormone analog LRH-A 220 μg / kg + dioxin maleate DOM 10 mg / kg + human chorionic gonadotropin (HCG) 2000 IU / kg. Simultaneously, male fish were given a second injection: luteinizing hormone-releasing hormone analog LRH-A 210 μg / kg + dioxin maleate DOM 5 mg / kg + human chorionic gonadotropin (HCG) 2000 IU / kg. IU / kg; the injection method and dosage are the same as the first and second injections; starting 6 hours after the third injection, check the spawning situation every 2 hours; 24 hours after the third injection, perform artificial spawning on female fish that have not yet spawned; S3. Insemination: Collect semen and add a special diluent at a volume ratio of 1:10. Then mix with eggs, adding 0.8 mL of diluted semen per gram of eggs. Next, add filtered clean water at a volume 5 times that of the sperm-egg mixture. Stir thoroughly and rinse repeatedly until the liquid is clear. Stir for 6 minutes and rinse 4 times to obtain fertilized eggs. S4. Fertilized egg incubation: The fertilized eggs are transferred to an incubation tank with a filter screen and incubated using micro-flow water. The incubation tank is also equipped with a microporous aeration device. Through the dual oxygenation of micro-flow water supplementation and microporous aeration, the dissolved oxygen in the incubation water is maintained at 7-9 mg / L. The incubation temperature is maintained at a constant 20 ℃, with a temperature difference fluctuation not exceeding ±0.5 ℃. The incubation time is 41 h. S5. Fry Rearing: After hatching, the fry are transferred to rearing tanks. Days 0-3 after hatching: The fry rely on their own yolk sac for nutrition and do not require feeding; allow them to rest. Days 4-7 after hatching: Feed with brine shrimp. Day 8 after hatching: Remove from the rearing tanks and release into the pond. Gradually add crushed formulated feed to the brine shrimp, gradually reducing the proportion of brine shrimp to acclimate their feeding habits. Stop feeding brine shrimp on day 12, allowing the fry to fully consume formulated feed. Day 13 after hatching: Feed with finely crushed feed twice daily, at 9:00 AM and 7:00 PM. Water Quality Management: Change 1 / 3-1 / 2 of the pond water every 6-8 days to ensure a stable aquatic environment for growth. Nutritional Fortification: Add 10% compound algae powder to the formulated feed once a week.

[0042] The special diluent mentioned in step S3 has the following composition: NaCl 6.3 g / L, KCl 0.25 g / L, CaCl2 0.15 g / L, glucose 1.4 g / L, NaHCO3 0.6 g / L, and the remainder is distilled water.

[0043] In this example, the fertilization rate was 73.9%, the hatching rate was 86.1%, and the survival rate of the fish fry after 30 days of rearing was 88.2%. Compared to Example 1, the special diluent for this comparative example did not contain reduced glutathione, arginine, or inositol.

[0044] Comparative Example 4 An artificial breeding method for the Oriental squid includes the following steps: S1. Selection of broodstock: During the natural breeding season, select female and male broodstock that are mature in gonads, robust and undamaged. The female broodstock should weigh over 50 g, have a soft, swollen abdomen, and a slightly protruding genital opening. The male broodstock should weigh over 20 g, have a slender body, a firm, non-swollen abdomen, and be robust individuals that produce milky white semen when the abdomen is gently pressed. S2. Induced Spawning Injection: A differentiated, fractionated injection method is used, with the second injection administered 12 hours after the first. The dosage for the first injection is 23 μg / kg of luteinizing hormone-releasing hormone analogue (LRH-A) + 2 mg / kg of dioxin maleate (DOM). The dosage for the second injection is 20 μg / kg of LRH-A + 10 mg / kg of dioxin maleate (DOM) + 400 IU / kg of human chorionic gonadotropin (HCG). Male fish receive a single injection simultaneously with the second injection for females, with a dosage of 10 μg / kg of LRH-A + 5 mg / kg of dioxin maleate (DOM) + 200 IU / kg of HCG. The injection method is an oblique injection at the base of the pectoral fin. The injection solution is prepared by dissolving the drugs in physiological saline, and the volume of the injection solution is 4... mL / kg; After injection, male and female parent fish were transferred to a closed, opaque tank at a 1:1 ratio to await spawning. A high dissolved oxygen environment was maintained continuously at a constant temperature of 20 ℃. Starting 10 hours after the second injection, the tank was opened every 2 hours to observe spawning. Once eggs were detected and spawning was observed, the eggs were removed and transferred to an incubation tank. After water changes, the fish continued to spawn in the original tank until spawning was complete. 24 hours after the second injection, a third injection was administered to female fish that had not yet spawned to induce spawning. The formula was: luteinizing hormone-releasing hormone analog LRH-A 220 μg / kg + dioxin maleate DOM 10 mg / kg + human chorionic gonadotropin (HCG) 2000 IU / kg. Simultaneously, male fish were given a second injection: luteinizing hormone-releasing hormone analog LRH-A 210 μg / kg + dioxin maleate DOM 5 mg / kg + human chorionic gonadotropin (HCG) 2000 IU / kg. IU / kg; the injection method and dosage are the same as the first and second injections; starting 6 hours after the third injection, check the spawning situation every 2 hours; 24 hours after the third injection, perform artificial spawning on female fish that have not yet spawned; S3. Insemination: Collect semen and add a special diluent at a volume ratio of 1:3. Then mix with eggs, adding 0.8 mL of diluted semen per gram of eggs. Next, add filtered clean water at a volume 5 times that of the sperm-egg mixture. Stir thoroughly and rinse repeatedly until the liquid is clear. Stir for 6 minutes and rinse 4 times to obtain fertilized eggs. S4. Fertilized egg incubation: The fertilized eggs are transferred to an incubation tank with a filter screen and incubated using micro-flow water. The incubation tank is also equipped with a microporous aeration device. Through the dual oxygenation of micro-flow water supplementation and microporous aeration, the dissolved oxygen in the incubation water is maintained at 7-9 mg / L. The incubation temperature is maintained at a constant 20 ℃, with a temperature difference fluctuation not exceeding ±0.5 ℃. The incubation time is 42 h. S5. Fry Rearing: After hatching, the fry are transferred to rearing tanks. Days 0-3 after hatching: The fry rely on their own yolk sac for nutrition and do not require feeding; allow them to rest. Days 4-7 after hatching: Feed with brine shrimp. Day 8 after hatching: Remove from the rearing tanks and release into the pond. Gradually add crushed formulated feed to the brine shrimp, gradually reducing the proportion of brine shrimp to acclimate their feeding habits. Stop feeding brine shrimp on day 12, allowing the fry to fully consume formulated feed. Day 13 after hatching: Feed with finely crushed feed twice daily, at 9:00 AM and 7:00 PM. Water Quality Management: Change 1 / 3-1 / 2 of the pond water every 6-8 days to ensure a stable aquatic environment for growth. Nutritional Fortification: Add 10% compound algae powder to the formulated feed once a week.

[0045] The special diluent mentioned in step S3 has the following composition: NaCl 6.3 g / L, KCl 0.25 g / L, CaCl2 0.15 g / L, glucose 1.4 g / L, NaHCO3 0.6 g / L, reduced glutathione 0.25 g / L, arginine 0.07 g / L, inositol 0.4 g / L, and the balance is distilled water.

[0046] In this example, the fertilization rate of sperm and egg was 62.8%, the hatching rate was 80.9%, and the survival rate of fish fry after 30 days of cultivation was 82.6%.

[0047] Compared to Example 1, the volume ratio of semen to special diluent in this comparative example is 1:3.

[0048] The above description is a further detailed explanation of the present invention in conjunction with specific implementation examples. It should not be considered that the specific implementation of the present invention is limited to these descriptions. For those skilled in the art, several simple deductions or substitutions can be made without departing from the concept of the present invention, and all such deductions or substitutions should be considered to fall within the protection scope of the present invention.

[0049] Those skilled in the art will readily understand that the above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A method for the artificial breeding of the Oriental squid, characterized in that, Includes the following steps: S1. Selection of parent fish: During the natural breeding season, select female and male parent fish that have mature gonads and are healthy and undamaged. S2. Induced Spawning Injection: A differentiated injection method is adopted for males and females. The second injection is given 12 hours after the first injection. The male fish is injected at the same time as the female fish for the second injection. After the injection, the male and female parent fish are transferred to a closed, light-proof tank at a 1:1 ratio to wait for spawning. The tank is continuously aerated to maintain a high dissolved oxygen environment and a constant temperature of 20 ℃. Starting from 10 hours after the second injection, the tank is opened every 2 hours to observe the spawning situation. After the eggs are detected and the spawning is found, the eggs are removed and transferred to the hatching tank. After the water is changed, the fish continue to wait for spawning in the original tank until the spawning is completed. S3. Insemination: Collect semen, add a special diluent, then mix with eggs, add filtered clean water, stir thoroughly and rinse repeatedly until the liquid is clear to obtain fertilized eggs; S4. Incubation of fertilized eggs: Transfer the fertilized eggs into an incubation tank with a filter and incubate them using a micro-flow water system. S5. Fry rearing: After the fry break through the membrane, transfer them to a rearing tank, feed them according to the stages and gradually acclimate them to their diet; In step S2, the formulation dosage of the first injection is luteinizing hormone-releasing hormone analog LRH-A2 3 μg / kg + dioxonone maleate DOM 2 mg / kg, the formulation dosage of the second injection is luteinizing hormone-releasing hormone analog LRH-A2 20 μg / kg + dioxonone maleate DOM 10 mg / kg + human chorionic gonadotropin HCG 400 IU / kg, and the formulation dosage of a single injection is luteinizing hormone-releasing hormone analog LRH-A2 10 μg / kg + dioxonone maleate DOM 5 mg / kg + human chorionic gonadotropin HCG 200 IU / kg; In step S2, 24 hours after the second injection, a third injection to induce spawning is given to female fish that have not yet laid eggs. The formula is: luteinizing hormone-releasing hormone analog LRH-A2 20 μg / kg + dioxin maleate DOM 10 mg / kg + human chorionic gonadotropin (HCG) 2000 IU / kg. At the same time, male fish are given a second injection, with the formula being: luteinizing hormone-releasing hormone analog LRH-A2 10 μg / kg + dioxin maleate DOM 5 mg / kg + human chorionic gonadotropin (HCG) 200 IU / kg. The injection method and dosage are the same as the first and second injections. Starting 6 hours after the third injection, spawning is checked every 2 hours. 24 hours after the third injection, artificial egg removal is performed on female fish that have not yet laid eggs. The special diluent mentioned in step S3 has the following composition: NaCl 5.8-6.5 g / L, KCl 0.15-0.3 g / L, CaCl2 0.1-0.2 g / L, glucose 0.9-1.8 g / L, NaHCO3 0.4-0.8 g / L, reduced glutathione 0.15-0.3 g / L, arginine 0.05-0.09 g / L, inositol 0.2-0.5 g / L, and the balance being distilled water; In step S3, the volume ratio of semen to special diluent is 1:8-12, the amount of filtered clean water added is 4-6 times the volume of the sperm-egg mixture, the stirring time is 2-10 minutes, and the rinsing is performed 3-4 times.

2. The artificial breeding method according to claim 1, characterized in that, In step S1, the female parent fish are selected from healthy individuals with a weight of over 50 g, a soft and swollen abdomen, and a slightly protruding genital opening; the male parent fish are selected from robust individuals with a weight of over 20 g, a slender body, a firm and non-swollen abdomen, and a milky white semen that flows out when the abdomen is gently pressed.

3. The artificial breeding method according to claim 1, characterized in that, In step S2, the injection method is an oblique injection at the base of the pectoral fin. The injection drug is dissolved in physiological saline to prepare an injection solution, and the injection solution volume is 4 mL / kg.

4. The artificial breeding method according to claim 1, characterized in that, The special diluent mentioned in step S3 has the following composition: NaCl 6.3 g / L, KCl 0.25 g / L, CaCl2 0.15 g / L, glucose 1.4 g / L, NaHCO3 0.6 g / L, reduced glutathione 0.25 g / L, arginine 0.07 g / L, inositol 0.4 g / L, and the balance is distilled water.

5. The artificial breeding method according to claim 1, characterized in that, The incubation temperature in step S4 is maintained at a constant 20 ℃, with a temperature difference fluctuation not exceeding ±0.5 ℃, and the incubation time is 40-42 h.

6. The artificial breeding method according to claim 1, characterized in that, The incubation tank described in step S4 is also equipped with a microporous aeration device, which maintains the dissolved oxygen in the incubation water at 7-9 mg / L through dual oxygenation via micro-flow water supplementation and microporous aeration.

7. The artificial breeding method according to claim 1, characterized in that, The specific process of fish fry cultivation described in step S5 is as follows: Days 0-3 after hatching: The fry rely on their own yolk sac for nutrition and do not need to be fed. They can be left to rest and develop. 4-7 days after membrane rupture: Feed with brine shrimp; Day 8 after hatching: Remove the fry from the rearing tank and put them into the pond. Add crushed compound feed to the brine shrimp in proportion, gradually reduce the proportion of brine shrimp to acclimate their feeding habits. Stop feeding brine shrimp on day 12 and let the fry eat only the artificial compound feed. Day 13 after membrane rupture: Feed finely chopped feed twice daily, at 9:00 AM and 7:00 PM. Water quality management: Change 1 / 3 to 1 / 2 of the pool water every 6-8 days to ensure a stable aquatic environment for growth; Nutritional fortification: Add 10% compound algae powder to the compound feed once a week.