Method for increasing aroma of tobacco leaf by absorbing amino acid in leaf and application thereof

Abstract

The present application belongs to the field of tobacco production and curing technology, and particularly relates to a method for increasing aroma of tobacco leaves by internal absorption of amino acids and application. The method comprises the following steps: naturally wilting harvested cigar leaves, obliquely cutting the petiole of the leaves to form an opening, and then directing internal absorption of amino acid internal absorption liquid through the vascular bundle opening, and finally obtaining cigar leaves with improved quality after water control, curing, stacking fermentation and aging. The present application can increase the effective deposition amount of amino acids in the leaves, significantly increase the total free amino acid accumulation amount, solve the technical problems of low absorption and utilization rate and less effective components retention of traditional exogenous application, directly increase the aroma precursor content of the cigar leaf, significantly improve the aroma amount and aroma quality of the cigar leaf, enrich the aroma level, improve the softness, delicateness and harmony of the smoke, reduce the offensive odor and irritation, stably improve the smoking quality of the cigar, and is suitable for quality improvement of cigar wrapper, cigar jacket and cigar core raw materials, and has good industrial application prospect.

Description

Technical Field

[0001] This invention belongs to the field of tobacco production and processing technology, and specifically relates to a method for increasing the aroma of tobacco leaves. Background Technology

[0002] The aroma of cigar tobacco leaves is one of the important indicators for evaluating the quality of cigar raw materials, directly affecting the style characteristics, sensory comfort, and industrial usability of cigar products. High-quality cigar tobacco leaves typically require good aroma quality, a rich aroma volume, good harmony, and low levels of off-flavors and harshness. However, domestically produced cigar tobacco leaves generally suffer from quality defects such as insufficient aroma, excessive bitterness, and noticeable off-flavors. Furthermore, because cigar products strive for "all-natural" qualities, it is difficult to improve their quality by adding external flavorings and fragrances. Therefore, it is urgent to explore effective ways to improve the intrinsic quality of tobacco leaves from the raw material processing stage.

[0003] In existing technologies, methods to enhance the aroma of cigar tobacco leaves mainly include optimizing cultivation management, improving harvest maturity, controlling fermentation processes, extending aging time, and applying exogenous nutrients. However, these methods still have the following problems: First, the aroma enhancement cycle is relatively long, relying heavily on later fermentation and aging to promote aroma formation, which is time-consuming, costly, and significantly affected by environmental conditions. Second, the utilization rate of exogenous application is low; in traditional spraying or soil application methods, active substances are easily lost and difficult to be efficiently absorbed by tobacco leaves, affecting the aroma enhancement effect. Third, the aroma improvement effect is unstable, affected by variety, part of the plant, maturity, and processing conditions; different treatment methods have significantly different aroma enhancement effects, making it difficult to form a stable and repeatable technical solution. Fourth, there is a lack of direct control technology for cigar tobacco leaves; most existing aroma enhancement methods target whole-plant cultivation management or later fermentation control, and there are still few technologies specifically targeting cigar tobacco leaves for internal treatment to promote the accumulation of aroma precursors and enhance aroma.

[0004] Amino acids are not only important nitrogenous metabolites in plant tissues, but also essential components for the formation of aroma precursors. During the curing, fermentation, and smoking of tobacco leaves, amino acids can form aroma substances such as aldehydes, ketones, and pyrazines through Maillard reactions, Stryker degradation reactions, and related transformation pathways. For example, the invention patent with publication number CN120267054A discloses a directional fermentation method to enhance the floral aroma of cigar tobacco leaves. By spraying a single amino acid solution onto the surface of rehydrated cigar tobacco leaves, the sensory quality of the cigar tobacco leaves is improved. However, the spraying method causes the amino acids to mainly adhere to the leaf surface. During subsequent fermentation and curing, they are easily lost due to surface moisture evaporation or operational losses, making it difficult for them to be efficiently transported into the mesophyll cells to participate in metabolic transformation, resulting in low actual absorption efficiency. The invention patent with publication number CN105029684A discloses a method for preparing cigar tobacco leaves using compound amino acid fermentation. The method involves first soaking the rehydrated cigar tobacco leaves in the stem, then spraying them with a compound amino acid aqueous solution at a weight ratio, and then fermenting them under high temperature and high humidity conditions. The preparation process is cumbersome, which increases the difficulty and cost of operation in industrial production. At the same time, the application method of mainly surface spraying and supplementing with stem soaking results in the amino acids mainly remaining on the surface layer of the tobacco leaves or the shallow layer of the veins, making it difficult to penetrate evenly into the mesophyll tissue, thus limiting the actual utilization rate.

[0005] In summary, existing methods all involve surface spraying after the tobacco leaves have rehydrated or before fermentation, failing to fully utilize the active transport of amino acids through physiological activity and transpiration during the post-harvest wilting period. Therefore, there is an urgent need to develop a simple, efficient, and stable method to enhance the characteristic aroma of cigar tobacco leaves, overcoming the shortcomings of existing technologies and significantly improving the quality of cigar tobacco leaves. Summary of the Invention

[0006] In response to the problems of low efficiency and poor stability in enhancing the aroma of cigar tobacco leaves and low absorption efficiency of exogenous aroma-enhancing precursors in existing technologies, this invention proposes a method and application for increasing the aroma of tobacco leaves by internal absorption of amino acids in the leaves. Specifically, it involves improving the aroma quality, increasing the aroma quantity, and improving the sensory quality by internal absorption of amino acids in cigar tobacco leaves.

[0007] The technical solution of this invention is implemented as follows:

[0008] This invention provides a method for enhancing the aroma of cigar tobacco leaves through the internal absorption of amino acids. The method involves introducing an internal liquid containing one or more amino acids into the interior of the cigar tobacco leaf through a cut in the midrib of the leaf petiole. This allows the amino acids to accumulate in the leaf tissue and participate in the conversion of aroma precursors during subsequent processing, fermentation, and aging. This improves the aroma quality, aroma quantity, and sensory comfort of the cigar tobacco leaves while reducing off-flavors and irritation. This method has advantages such as high absorption efficiency, stable processing results, simple operation, and suitability for widespread application, and can be used to improve the quality of cigar tobacco raw materials.

[0009] Based on this, the present invention provides a method for increasing the aroma of tobacco leaves by absorbing amino acids from the leaves, the steps of which are as follows:

[0010] (1) Take the harvested cigar tobacco leaves and perform natural withering pretreatment;

[0011] (2) Cut the petiole of the wilted cigar tobacco leaf obliquely, make an incision at the base of the main vein of the leaf, and immerse the cut end in an amino acid systemic solution for systemic absorption;

[0012] (3) After the leaves are dehydrated after internal absorption, they are successively processed, piled up for fermentation and aging to obtain cigar tobacco leaves with increased aroma.

[0013] This invention introduces an amino acid solution into the interior of cigar tobacco leaf tissue, allowing the amino acids to accumulate in the leaves and participate in the aroma precursor conversion during subsequent processing, fermentation, or aging. This improves the aroma quality, aroma quantity, and sensory comfort of cigar tobacco leaves, reduces off-flavors and irritation, and enhances the industrial usability and raw material value of cigar tobacco leaves.

[0014] Preferably, in step (1) above, the cigar tobacco leaves are middle or upper leaves with uniform maturity and size; the natural withering time is 10-18 h.

[0015] Preferably, the amino acid internal absorption solution in step (2) above includes an amino acid solution and a NaCl solution, the mass concentration of the amino acid is 0.1% to 0.5%, the mass concentration of the NaCl is 0.9%, and the cut is oblique (full cut).

[0016] Preferably, the mass concentration of the above-mentioned amino acid is 0.25%.

[0017] Preferably, the amino acid is selected from any one or more of aspartic acid, phenylalanine, proline, alanine, serine, and glycine.

[0018] Preferably, the internal absorption time in step (2) above is 12 to 24 hours, the internal absorption temperature is 20°C to 30°C, and the relative humidity is 70% to 90%.

[0019] Preferably, in step (3) above, the preparation is conventional cigar drying, which includes the withering period, yellowing period, browning period and dry stem period: during the withering period, the temperature of the drying room is controlled at 20-28℃ and the relative humidity is 70%-85%, lasting for 2-4 days; during the yellowing period, the temperature is controlled at 25-31℃ and the relative humidity is 75%-85%, lasting for 5-8 days; during the browning period, the temperature is controlled at 28-34℃ and the relative humidity is 65%-80%, lasting for 4-8 days; during the dry stem period, the temperature is controlled at 32-37℃ and the relative humidity is 50%-70%, lasting for 4-7 days, until the leaves and main veins are dry and the moisture content of the tobacco leaves drops to 16%-20%; the total drying time is 20-30 days.

[0020] The stacking fermentation is a conventional method for cigar tobacco stacking fermentation. After drying, the tobacco leaves are rehydrated to a moisture content of 25%–35%, and piled at 100–200 kg / stack. The stacks are covered with cotton quilts, linen, or straw mats for insulation and moisture retention. The fermentation environment temperature is 20–30℃, and the relative humidity is 70%–80%. The temperature at the center of the stack is monitored in real time. When the temperature at the center of the stack rises to 35–40℃, the stacks are turned over. During turning, the stacks are rearranged in the following order: outer layer to inner layer, inner layer to outer layer, upper layer to lower layer, and lower layer to upper layer. The stacks are turned over every 2–5 days, for a total of 2–5 times. Fermentation ends when the temperature of the stack no longer rises significantly, the moisture content of the tobacco leaves drops to 18%–22%, and the green and ammonia odors of the tobacco leaves are significantly reduced.

[0021] The ambient temperature for the alcohol aging process is 18–20°C, the humidity (RH) is 60%–65%, and there is slight continuous ventilation.

[0022] The specific steps are as follows:

[0023] 1. Selection of Cigar Tobacco Leaves

[0024] The preferred choice is fresh leaves that have been harvested, have intact main veins, and are free from mechanical damage.

[0025] Further preferred are middle or upper leaves with uniform maturity and uniform leaf size.

[0026] 2. Internal absorption treatment method for blades

[0027] The leaf internal absorption treatment involves allowing the amino acid solution to be absorbed into the leaf tissue through the cigar tobacco leaf. Specifically, the midrib incision internal absorption method can be used: after forming an incision at the base of the midrib of the leaf, the incision end is placed in the amino acid internal absorption solution, allowing the solution to be introduced into the leaf tissue along the midrib.

[0028] 3. Systemic treatment conditions

[0029] The internal absorption treatment time is 12 to 24 hours; the treatment environment temperature is 20°C to 30°C; and the relative humidity is 70% to 90%.

[0030] 4. Screening of optimal concentration for systemic absorption solution

[0031] The amino acid systemic solution comprises one or more amino acids, selected from 17 abundant free amino acids found in tobacco leaves, preferably amino acids closely related to the formation of tobacco aroma precursors, such as aspartic acid, phenylalanine, etc., one or more of these. The amino acid systemic concentration is set with a concentration gradient (0.1%, 0.15%, 0.2%, 0.25%, and 0.3%) based on the solubility of the amino acids for absorption.

[0032] 5. Follow-up processing

[0033] After internal absorption treatment at the optimal concentration, the cigar tobacco leaves are first dehydrated, and then subjected to conventional blending, stacking fermentation, or aging. Through subsequent fermentation and aging, the amino acids absorbed in the leaves further participate in the conversion of aroma precursors, thereby improving the aroma quality of the cigar tobacco leaves.

[0034] Secondly, the aforementioned method of increasing tobacco aroma through the absorption of amino acids within the leaves is applied to improving tobacco quality.

[0035] Preferably, the tobacco leaves mentioned above are cigar tobacco leaves, and the improvement in tobacco leaf quality is achieved by increasing the total free amino acid content of the tobacco leaves and improving the sensory quality of the smoke; the improvement in sensory quality includes at least one of the following: enhanced umami, sweetness, and aroma, and reduced irritation and off-flavors.

[0036] Improving tobacco quality includes increasing the total free amino acid content of tobacco leaves, enhancing umami, sweetness, and aroma, and reducing irritation and off-flavors.

[0037] The present invention has the following beneficial effects:

[0038] 1. This invention provides a method for enhancing tobacco aroma through the internal absorption of amino acids within the leaves. This method directly introduces amino acids into the tissue of cigar tobacco leaves, increasing the effective deposition of amino acids in the leaves. This directly increases the content of aroma precursors in cigar tobacco leaves, providing a precursor basis for the formation of subsequent aroma substances. The tobacco leaves can efficiently and uniformly enrich key aroma precursor amino acids such as aspartic acid and phenylalanine, significantly increasing the accumulation of total free amino acids. This fundamentally solves the technical problems of low absorption and utilization rates and insufficient retention of effective components associated with traditional exogenous application.

[0039] 2. This invention is beneficial for improving the quality and quantity of aroma. Amino acids can participate in a series of aroma-forming reactions during the modulation, fermentation, and aging processes, thereby enhancing the aroma complexity and richness of cigar tobacco leaves. It can also improve sensory comfort; by inhaling amino acids within the leaves, the smoothness, fineness, and harmony of the smoke can be improved to a certain extent, enhancing the quality and quantity of aroma, reducing off-flavors and irritation, and improving the cigar smoking experience.

[0040] 3. The aroma enhancement method of this invention is stable and controllable. Compared with simple surface spraying, the leaf-based internal absorption method reduces the loss of the treatment liquid, improves the absorption and utilization rate, and makes it easier to control the treatment volume, treatment time, and treatment conditions, which is conducive to forming a stable aroma enhancement effect. This invention is suitable for improving the quality of cigar raw materials, especially for improving the quality of cigar wrappers, binders, or fillers, and has good production application value. Attached Figure Description

[0041] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0042] Figure 1 The figures show the phenotypes of tobacco leaves; Figure A shows cigar tobacco leaves that have withered naturally for 12 hours, and Figure B shows cigar tobacco leaves that have absorbed amino acid solution.

[0043] Figure 2 The change in amino acid content of tobacco leaves after the addition of aspartic acid. Detailed Implementation

[0044] The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0045] Unless otherwise specified, the experimental methods used in the following experimental examples are conventional methods; the materials and reagents used are commercially available unless otherwise specified.

[0046] This invention provides a method for increasing the aroma of tobacco leaves by absorbing amino acids from the leaves. The overall scheme is as follows:

[0047] 1. Select cigar tobacco leaves of suitable maturity and let them wither naturally in a cool place for 12 hours;

[0048] 2. Prepare an amino acid solution + 0.9% NaCl solution;

[0049] 3. Slant the stems of the wilted cigar leaves and soak them in the prepared solution to absorb the nutrients (e.g., ...). Figure 1 (as shown)

[0050] 4. The processed cigar tobacco leaves are then seasoned and fermented;

[0051] 5. Obtain cigar tobacco leaves with enhanced aroma.

[0052] The amino acid internal liquid of the present invention includes one or more amino acids, selected from 17 free amino acids that are abundant in tobacco leaves, preferably amino acids that are closely related to the formation of tobacco aroma precursors, such as one or more of aspartic acid and phenylalanine.

[0053] The following examples further define the method for increasing tobacco aroma by inhaling amino acids from the leaves; details are provided in the examples.

[0054] Example 1: A method for increasing tobacco aroma through the internal uptake of aspartic acid (Asp) in tobacco leaves

[0055] 1. Selection of Cigar Tobacco Leaves

[0056] The preferred choice is fresh leaves that have been harvested, have intact main veins, and are free from mechanical damage.

[0057] Further preferred are the middle leaves with uniform maturity and uniform leaf size.

[0058] 2. Internal absorption treatment method for blades

[0059] The leaf internal absorption treatment involves allowing the amino acid solution to be absorbed into the leaf tissue through the cigar tobacco leaf. Specifically, the midrib incision internal absorption method can be used: after forming an incision at the base of the midrib of the leaf, the incision end is placed in the amino acid internal absorption solution, allowing the solution to be introduced into the leaf tissue along the midrib.

[0060] 3. Systemic treatment conditions

[0061] The internal absorption treatment time is 12 hours;

[0062] The processing environment temperature is 20℃;

[0063] The relative humidity of the environment is 70%.

[0064] 4. Screening of optimal concentration for systemic absorption solution

[0065] The amino acid systemic solution comprises one or more amino acids, selected from 17 abundant free amino acids found in tobacco leaves, with aspartic acid, which is closely related to the formation of aroma precursors in tobacco leaves, being a preferred candidate. The amino acid systemic concentration is set according to a concentration gradient based on the solubility of the amino acids. Taking aspartic acid as an example, with a solubility of 0.3% (at room temperature), the concentration gradient is set to 0.1%, 0.15%, 0.2%, 0.25%, and 0.3%. The optimal concentration is determined by detecting the amino acids. Figure 2 ).

[0066] from Figure 2It can be seen that aspartic acid in tobacco leaves increases significantly at a concentration of 0.25%, which is an important component of cigar aroma.

[0067] 5. Follow-up processing

[0068] After internal absorption treatment at the optimal concentration, the cigar tobacco leaves are first dehydrated, and then subjected to conventional blending, stacking fermentation, or aging. Through subsequent fermentation and aging, the amino acids absorbed in the leaves further participate in the conversion of aroma precursors, thereby improving the aroma quality of the cigar tobacco leaves.

[0069] Example 2: A method for increasing tobacco aroma through the internal uptake of phenylalanine (Phe) by leaf leaves

[0070] 1. Selection of Cigar Tobacco Leaves

[0071] The preferred choice is fresh leaves that have been harvested, have intact main veins, and are free from mechanical damage.

[0072] Further preferred are upper leaves with uniform maturity and uniform leaf size.

[0073] 2. Internal absorption treatment method for blades

[0074] The leaf internal absorption treatment involves allowing the amino acid solution to be absorbed into the leaf tissue through the cigar tobacco leaf. Specifically, the midrib incision internal absorption method can be used: after forming an incision at the base of the midrib of the leaf, the incision end is placed in the amino acid internal absorption solution, allowing the solution to be introduced into the leaf tissue along the midrib.

[0075] 3. Systemic treatment conditions

[0076] The internal absorption treatment time is 24 hours;

[0077] The processing environment temperature is 30℃;

[0078] The relative humidity of the environment is 90%.

[0079] 4. Screening of optimal concentration for systemic absorption solution

[0080] The amino acid internal solution includes one or more amino acids, selected from 17 free amino acids that are abundant in tobacco leaves, preferably phenylalanine, which is closely related to the formation of aroma precursors in tobacco leaves, and the concentration of phenylalanine is set at 0.25%.

[0081] 5. Follow-up processing

[0082] After internal absorption treatment at the optimal concentration, the cigar tobacco leaves are first dehydrated, and then subjected to conventional blending, stacking fermentation, or aging. Through subsequent fermentation and aging, the amino acids absorbed in the leaves further participate in the conversion of aroma precursors, thereby improving the aroma quality of the cigar tobacco leaves.

[0083] Example 3: A method for enhancing tobacco aroma through the internal absorption of mixed amino acids from tobacco leaves

[0084] 1. Selection of Cigar Tobacco Leaves

[0085] The preferred choice is fresh leaves that have been harvested, have intact main veins, and are free from mechanical damage.

[0086] Further preferred are upper leaves with uniform maturity and uniform leaf size.

[0087] 2. Internal absorption treatment method for blades

[0088] The leaf internal absorption treatment involves allowing the amino acid solution to be absorbed into the leaf tissue through the cigar tobacco leaf. Specifically, the midrib incision internal absorption method can be used: after forming an incision at the base of the midrib of the leaf, the incision end is placed in the amino acid internal absorption solution, allowing the solution to be introduced into the leaf tissue along the midrib.

[0089] 3. Systemic treatment conditions

[0090] The internal absorption treatment time is 20 hours;

[0091] The processing environment temperature is 25℃;

[0092] The relative humidity of the environment is 80%.

[0093] 4. Screening of optimal concentration for systemic absorption solution

[0094] The amino acid internal solution comprises one or more amino acids, selected from 17 abundant free amino acids found in tobacco leaves. A mixed solution of aspartic acid, phenylalanine, proline, alanine, serine, and glycine, which are closely related to the formation of aroma precursors in tobacco leaves, is selected. The concentrations of aspartic acid and phenylalanine are 0.1%, and the concentrations of proline, alanine, serine, and glycine are 0.5%.

[0095] 5. Follow-up processing

[0096] After internal absorption treatment at the optimal concentration, the cigar tobacco leaves are first dehydrated, and then subjected to conventional blending, stacking fermentation, or aging. Through subsequent fermentation and aging, the amino acids absorbed in the leaves further participate in the conversion of aroma precursors, thereby improving the aroma quality of the cigar tobacco leaves.

[0097] Comparative Example: A Method for Enhancing Tobacco Aroma Through Inhalation into the Leaf

[0098] 1. Selection of Cigar Tobacco Leaves

[0099] The preferred choice is fresh leaves that have been harvested, have intact main veins, and are free from mechanical damage.

[0100] Further preferred are the middle leaves with uniform maturity and uniform leaf size.

[0101] 2. Internal absorption treatment method for blades

[0102] The leaf internal absorption treatment involves absorbing 0.9% NaCl solution into the leaf tissue through the cigar tobacco leaf. Specifically, the midrib incision internal absorption method can be used: after forming an incision at the base of the midrib of the leaf, the incision end is placed in 0.9% NaCl, allowing the solution to be introduced into the leaf tissue along the midrib.

[0103] 3. Systemic treatment conditions

[0104] The internal absorption treatment time is 12 hours;

[0105] The processing environment temperature is 20℃;

[0106] The relative humidity of the environment is 70%.

[0107] 4. Follow-up processing

[0108] After internal absorption treatment at the optimal concentration, the cigar tobacco leaves are first dehydrated, and then subjected to conventional blending, stacking fermentation, or aging. Through subsequent fermentation and aging, the amino acids absorbed in the leaves further participate in the conversion of aroma precursors, thereby improving the aroma quality of the cigar tobacco leaves.

[0109] Example of implementation results:

[0110] The amino acid content of the tobacco leaves prepared according to Example 1, Example 2 and the comparative example was detected. The amino acid content is shown in Table 1.

[0111] Table 1 Amino acid content of tobacco leaves after drying under different treatments

[0112]

[0113] Table 1 shows that exogenous amino acid treatment significantly altered the composition and content of free amino acids in cigar filler leaves. Compared to the control (CK), the total free amino acid content increased from 17901.81 to 18835.80 after Asp treatment, an increase of approximately 5.2%; after Phe treatment, it increased to 25365.46, an increase of approximately 41.7% compared to the CK. P<0.05). Looking at different flavor profiles, the Asp treatment increased the total umami amino acid content from 3169.99 to 14824.98, an increase of approximately 3.68 times, with Asp and Glu reaching 10758.96 and 4066.02 respectively; while the total sweet amino acid content decreased from 10556.10 to 2084.42, a decrease of approximately 80.3%, and the total bitter amino acid content decreased from 4121.59 to 1886.42, a decrease of approximately 54.2%. In contrast, the Phe treatment significantly increased the total umami, sweet, and bitter amino acids, reaching 4668.01, 14338.67, and 6320.21 respectively, representing increases of 47.3%, 35.8%, and 53.3% compared to the control (CK), with the most significant increase in sweet amino acids. Therefore, Asp treatment mainly promotes the accumulation of umami amino acids while inhibiting sweet and bitter amino acids; while Phe treatment can comprehensively increase the content of free amino acids, especially promoting the accumulation of sweet and bitter amino acids.

[0114] Table 2 Sensory evaluation results of tobacco leaves under different treatments

[0115]

[0116] The sensory quality results of tobacco leaves before and after fermentation are shown in Table 2. As can be seen from Table 2, after adding exogenous amino acids, there was no significant difference in concentration and combustibility between the treatments and the control group. However, the aroma quality and aroma quantity were improved compared with the control group, the irritation decreased, the off-flavors were reduced, the sweetness increased, and the aftertaste was more comfortable. The sensory quality of tobacco leaves after fermentation was further improved in each treatment.

[0117] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A method for increasing the aroma of tobacco leaves by absorbing amino acids from the leaves, characterized in that, The steps are as follows: (1) Take the harvested cigar tobacco leaves and perform natural withering pretreatment; (2) Cut the petiole of the wilted cigar tobacco leaf obliquely, make an incision at the base of the main vein of the leaf, and immerse the cut end in an amino acid systemic solution for systemic absorption; (3) After the leaves are dehydrated after internal absorption, they are successively processed, piled up for fermentation and aging to obtain cigar tobacco leaves with increased aroma.

2. The method for increasing tobacco aroma by absorbing amino acids from leaves according to claim 1, characterized in that: In step (1), the cigar tobacco leaves are middle or upper leaves with uniform maturity and size; the natural withering time is 10-18 h.

3. The method for increasing tobacco aroma by absorbing amino acids from leaves according to claim 2, characterized in that: The amino acid internal absorption solution in step (2) contains an amino acid solution and a NaCl solution. The mass concentration of the amino acid is 0.1% to 0.5%, the mass concentration of the NaCl is 0.9%, and the incision is a full cut.

4. The method for increasing tobacco aroma by absorbing amino acids from leaves according to claim 3, characterized in that: The amino acid is selected from any one or more of aspartic acid, phenylalanine, proline, alanine, serine, and glycine.

5. The method for increasing tobacco aroma by absorbing amino acids from leaves according to claim 4, characterized in that: In step (2), the internal absorption time is 12 to 24 hours, the internal absorption temperature is 20°C to 30°C, and the relative humidity is 70% to 90%.

6. The method for increasing tobacco aroma by absorbing amino acids from leaves according to claim 5, characterized in that: In step (3), the process is modified to be the drying process for cigars. The total drying time is 20 to 30 days, including four drying stages: withering, yellowing, browning and drying.

7. The method for increasing tobacco aroma by absorbing amino acids from leaves according to claim 6, characterized in that: In step (3), the stacking fermentation is carried out at a rate of 100-200 kg / stack, with an ambient temperature of 20-30℃ and a relative humidity of 70%-80%. When the temperature at the center of the stack rises to 35-40℃, the stack is turned over. The stack is turned over once every 2-5 days, for a total of 2-5 times.

8. The method for increasing tobacco aroma by absorbing amino acids from leaves according to claim 7, characterized in that: The ambient temperature for the alcoholization process in step (3) is 18-20°C, the humidity is 60%-65%, and there is continuous slight ventilation.

9. The application of the method for increasing tobacco aroma by absorbing amino acids from leaves according to any one of claims 1-8 in improving tobacco quality.

10. The application according to claim 9, characterized in that: The tobacco leaves are cigar tobacco leaves, and the quality of the tobacco leaves is characterized by at least one of the following: increased total free amino acid content, improved umami, sweetness and aroma, and reduced irritation and off-flavors.

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