A poultry olfactory bulb sampling device

Abstract

The poultry olfactory bulb sampling device comprises a euthanasia assembly; a cutting assembly installed on the top surface of the euthanasia assembly; and a dissection assembly installed on the top surface of the euthanasia assembly. Advantages of the present application: through the adjustable cutting assembly, the device can realize stable limiting of poultry of different sizes, then accurately control the cutting position of the head, and avoid the deviation of manual cutting; at the same time, the leveling and rotating design of the dissection platform, combined with the multi-dimensional head, neck and beak fixing structure, can realize stable fixation and posture adjustment of the poultry head, ensure that the head does not shake during the dissection process, the operator can accurately position the olfactory bulb tissue, and carry out dissection and sampling, the sample integrity is also significantly guaranteed, and the problem that the sample quality is uneven in manual operation is effectively solved.

Description

Technical Field

[0001] This invention relates to a sampling device, and more particularly to a poultry olfactory bulb sampling device. Background Technology

[0002] In current poultry disease prevention and control and neuroscience research, olfactory bulb tissue is a crucial sample carrier: on the one hand, pathogens of many poultry respiratory diseases preferentially colonize the olfactory epithelium and olfactory bulb tissue. For example, infectious bursal virus can infect the host through the nasal cavity and first complete its initial replication in the olfactory bulb region. Sampling of olfactory bulb tissue can significantly improve the sensitivity of early disease detection, enabling early detection and early treatment of diseases. On the other hand, research on the development and functional mechanisms of the poultry olfactory system also requires obtaining complete and uncontaminated olfactory bulb tissue samples to ensure the reliability and reproducibility of experimental results.

[0003] Currently, sampling of the olfactory bulb in poultry is still mainly done manually, which presents many inconveniences: the olfactory bulb tissue is tiny and located at the front of the poultry's cranial cavity, requiring high precision in sampling. However, in manual operation, different operators have significant differences in cutting positions and dissection techniques, and it is difficult to achieve stable positioning when manually fixing the head. This easily leads to problems such as cutting position deviations, damage to the olfactory bulb tissue caused by head shaking during dissection, and incorrect sampling sites, resulting in poor sample integrity and low pass rates, which seriously affect the accuracy of subsequent testing or research.

[0004] To address the above issues, we provide a poultry olfactory bulb sampling device. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of the existing technology and provide a poultry olfactory bulb sampling device.

[0006] The objective of this invention is achieved as follows:

[0007] A poultry olfactory bulb sampling device, including an euthanasia component;

[0008] A cutting assembly is installed on the top surface of the euthanasia assembly;

[0009] An autopsy assembly is mounted on the top surface of the euthanasia assembly.

[0010] Furthermore, the euthanasia assembly includes an euthanasia chamber, an euthanasia chamber door, an air supply plate, air supply nozzles, and an exhaust rod. The euthanasia chamber is a hollow chamber structure with an open front side. An euthanasia chamber door is installed on the front side of the euthanasia chamber. An air supply plate is installed on the top surface of the inner cavity of the euthanasia chamber. Several air supply nozzles are installed on the bottom surface of the air supply plate. Exhaust rods are installed on both sides of the bottom surface of the inner cavity of the euthanasia chamber.

[0011] Furthermore, the cutting assembly includes a cutting box, a cutting box door, a lower door panel, a disinfection component, a suspension base, a suspension telescopic rod, a suspension component, and a cutting component. The cutting box is provided on the top surface of the euthanasia assembly. The cutting box is a hollow box structure with an open front side. A cutting box door is installed on the upper part of the front side of the cutting box, and a lower door panel is installed on the lower part of the front side of the cutting box. A suspension base is provided on the bottom surface of the inner cavity of the cutting box, and a suspension telescopic rod is installed on the top surface of the suspension base. A suspension component is installed at the output end of the suspension telescopic rod. A cutting component is installed on the upper part of the inner cavity of the cutting box, and the cutting component cuts off the head of the poultry.

[0012] Furthermore, the disinfection component includes a disinfection plate, disinfection nozzles, a disinfection box, and a disinfection pump. The disinfection plate is installed on the top surface of the inner cavity of the cutting box, and a plurality of disinfection nozzles are provided on the bottom surface of the disinfection plate. The disinfection box is provided on the top surface of the cutting box, and a disinfection pump is installed on one side of the disinfection box. The input end of the disinfection pump is connected to the disinfection box, and the output end of the disinfection pump is connected to the disinfection plate through pipes.

[0013] Furthermore, the suspension component includes an upper suspension plate, a lower suspension plate, an intermediate plate, a limit motor, and a limit rod. Both the upper suspension plate and the lower suspension plate are U-shaped structures. An intermediate plate is provided at the output end of the suspension telescopic rod. The upper suspension plate and the lower suspension plate are respectively provided on the upper and lower sides of the other end of the intermediate plate. The U-shaped ends of the upper suspension plate and the lower suspension plate are both oriented away from the intermediate plate. A limit motor is installed on the bottom surface of the lower suspension plate, and the output shaft of the limit motor extends to the top surface of the lower suspension plate and is provided with a limit rod.

[0014] Furthermore, the suspension component also includes a lower extension rod, an arc-shaped plate, a suspension linear motor platform, a fixed telescopic rod, and a fixing plate. The lower extension rod is provided in the middle of the bottom surface of the lower suspension plate, and arc-shaped plates are provided on both sides of the lower end of the lower extension rod. Suspension linear motor platforms are installed in the middle of both sides of the inner cavity of the cutting box. A fixed telescopic rod is installed at the output end of the suspension linear motor platform, and a fixing plate is provided at the output end of the fixed telescopic rod.

[0015] Furthermore, the cutting component includes a cutting linear motor platform, a cutting cylinder, a cutting blade, and a mating block. The upper parts of both sides of the inner cavity of the cutting box are respectively equipped with a cutting linear motor platform. A cutting cylinder is installed at the output end of the cutting linear motor platform. A cutting blade is provided at the output end of one of the cutting cylinders, and a mating block is provided at the output end of the other cutting cylinder. A cutting groove adapted to the cutting blade is opened on the surface of the mating block.

[0016] Furthermore, each corner of the bottom surface of the euthanasia component is provided with a support leg.

[0017] Furthermore, the dissection assembly includes a dissection frame, a leveling component, a rotating component, a dissection platform, and a fixing component. The top surface of the euthanasia assembly is provided with a dissection frame. A leveling component is installed in the middle of the bottom surface of the inner cavity of the dissection frame. A rotating component is installed on the top surface of the leveling component. A dissection platform is installed at the output end of the rotating component. A fixing component is installed in the middle of the top surface of the dissection platform. The fixing component fixes the poultry's head so that the olfactory bulb can be sampled during dissection.

[0018] Furthermore, the leveling component includes leveling push rods and leveling ball head connectors. Four leveling push rods are installed in the middle of the bottom surface of the anatomical frame cavity. The four leveling push rods are distributed in a square. The output end of the leveling push rod is equipped with a leveling ball head connector. The other end of the leveling ball head connector is fixedly connected to each corner of the top surface of the rotating component.

[0019] Furthermore, the rotating component includes a rotating housing, a rotating housing cover, a rotating motor, a supporting slide rail, a supporting slider, a fixing rod, a fixing spring, and a fixing magnet. The other ends of the four leveling ball joint connectors are fixedly connected to the corners of the bottom surface of the rotating housing. The rotating housing is a hollow housing structure with an open front side. A rotating housing cover is installed on the front side of the rotating housing. A rotating motor is installed on the top surface of the inner cavity of the rotating housing. The output end of the rotating motor extends to the upper part of the top surface of the rotating housing and is provided with the dissection platform. A supporting slide rail is provided on the bottom surface of the dissection platform. Several supporting sliders are provided on the top surface of the rotating housing. The supporting sliders are slidably connected to the supporting slide rail. A fixing rod is provided on the top surface of the rotating housing. A fixing groove is opened on the upper part of the fixing rod. Several fixing springs are provided inside the fixing groove. A fixing magnet is provided at the other end of the fixing spring.

[0020] Further, the fixing components include a head fixing block, a neck fixing block, a beak fixing block, a fixing frame, a neck telescopic rod, an inclined connecting seat, a neck fixing plate, a skin fixing plate, a beak fixing plate, a rotating seat, a drive rod, a drive push rod, a drive seat, a drive slider, and a drive slide rail. A head fixing block is located in the center of the top surface of the dissection platform. A head fixing groove is formed through the top surface of the head fixing block. Skin fixing plates are located on both sides of the top surface of the head fixing block. Neck fixing blocks and beak fixing blocks are located on the rear and front / rear sides of the head fixing block, respectively. A fixing frame is located on the top surface of the dissection platform. The top of the fixation frame is equipped with a neck telescopic rod, the output end of which is provided with an inclined connecting seat, and the bottom end of which is provided with a neck fixing plate. The top surface of the beak fixing block is provided with a pair of rotating seats, and the beak fixing plate is rotatably connected between the two rotating seats. A pair of drive rods are provided on the outer side of the beak fixing plate, and a drive slide rail is provided on the outer side of the drive rod along its length direction. A drive slider is slidably connected in the drive slide rail. The top surface of the dissection platform is equipped with a drive push rod, and the output end of the drive push rod is provided with a drive seat. The drive seat is rotatably connected to the drive slider.

[0021] Furthermore, a placement component is installed on one side of the euthanasia component.

[0022] Furthermore, the placement assembly includes a placement top plate, a placement push rod, a placement slide, a placement motor, a rotating plate, a connecting plate, a placement frame, a placement box, and placement magnets. The placement top plate is provided on the rear side of the cutting box. A placement push rod is installed on the bottom surface of the placement top plate. A placement slide is provided at the output end of the placement push rod. A placement motor is installed inside the placement slide. The output end of the placement motor extends to the outside of the placement slide and is provided with a rotating plate. A connecting plate is provided at the other end of the rotating plate. A placement frame is rotatably connected to one side of the connecting plate. A placement box is provided in the middle of the placement frame. Several placement magnets are installed on the top surface of the connecting plate near the placement frame.

[0023] Advantages of this invention:

[0024] 1. Through adjustable cutting components, this equipment can achieve stable positioning of poultry of different sizes, and then precisely control the cutting position of the head, avoiding the deviations of manual cutting. At the same time, the leveling and rotation design of the dissection platform, combined with the multi-dimensional head, neck and beak fixation structure, can achieve stable fixation and posture adjustment of poultry decapitation, ensuring no head shaking during dissection. Operators can accurately locate the olfactory bulb tissue and perform dissection sampling, and the integrity of the sample is also significantly guaranteed, effectively solving the problem of inconsistent sample quality in manual operation.

[0025] 2. This invention integrates the euthanasia component, the cutting component, and the dissection component into one unit, realizing a streamlined operation from execution to cutting and then to dissection and sampling, reducing sample transfer and contamination, and improving sampling efficiency. Attached Figure Description

[0026] Figure 1 This is a schematic diagram of a poultry olfactory bulb sampling device.

[0027] Figure 2 Diagram showing the opening of the euthanasia box door.

[0028] Figure 3 This is a diagram showing the opening of the cutting box door.

[0029] Figure 4 A schematic diagram of the cutting component structure hidden in the cutting box door.

[0030] Figure 5 This is a schematic diagram of the dissected components and their placement.

[0031] Figure 6 A schematic diagram of the anatomical assembly structure for opening the steering gearbox door.

[0032] Figure 7 This is an enlarged view of point A.

[0033] Figure 8 This is a schematic diagram of the cross-sectional structure of the anatomical component.

[0034] In the picture:

[0035] 1. Euthanasia components; 11. Euthanasia chamber; 12. Euthanasia chamber door; 13. Air supply plate; 14. Air supply nozzle; 15. Exhaust rod;

[0036] 2. Supporting leg;

[0037] 3. Cutting components; 31. Cutting box body; 32. Cutting box door; 33. Lower door panel; 34. Sterilization component; 341. Sterilization plate; 342. Sterilization nozzle; 343. Sterilization box; 344. Sterilization pump; 35. Suspension base; 36. Suspension telescopic rod; 37. Suspension component; 3701. Upper suspension plate; 3702. Lower suspension plate; 3703. Intermediate plate; 3704. Limit motor; 3705. Limit rod; 3706. Lower extension rod; 3707. Arc plate; 3708. Suspension linear motor; 3709. Fixed telescopic rod; 3710. Fixed plate; 38. Cutting component; 381. Cutting linear motor platform; 382. Cutting cylinder; 383. Cutting blade; 384. Mating block;

[0038] 4. Anatomical Components, 41. Anatomical Frame, 42. Leveling Component, 421. Leveling Push Rod, 422. Leveling Ball Joint Connector, 43. Rotating Component, 431. Rotating Box, 432. Rotating Box Cover, 433. Rotating Motor, 434. Support Rail, 435. Support Slider, 436. Fixing Rod, 437. Fixing Spring, 438. Fixing Magnet, 44. Anatomical Platform, 45. Fixing Component, 4501. Head Fixation 4502. Neck fixing block, 4503. Beak fixing block, 4504. Fixing frame, 4505. Neck telescopic rod, 4506. Inclined connecting seat, 4507. Neck fixing plate, 4508. Skin fixing plate, 4509. Beak fixing plate, 4510. Rotating seat, 4511. Drive rod, 4512. Drive push rod, 4513. Drive seat, 4514. Drive slider, 4515. Drive slide rail;

[0039] 5. Place components; 51. Place top plate; 52. Place push rod; 53. Place slide; 54. Place motor; 55. Rotating plate; 56. Connecting plate; 57. Place frame; 58. Place box; 59. Place magnet. Detailed Implementation

[0040] To enable those skilled in the art to better understand the technical solutions in this application, the technical solutions in the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments in this application, all other embodiments obtained by those of ordinary skill in the art without creative effort should fall within the scope of protection of this application.

[0041] This application provides a poultry olfactory bulb sampling device.

[0042] Example 1, as Figure 1-8 As shown.

[0043] A poultry olfactory bulb sampling device includes an euthanasia component 1 for euthanizing poultry.

[0044] Each corner of the bottom surface of the euthanasia component 1 is provided with a support leg 2 to support the entire sampling device.

[0045] The euthanasia assembly 1 includes an euthanasia box 11, an euthanasia box door 12, an air supply plate 13, an air supply nozzle 14, and an exhaust rod 15. The euthanasia box 11 is a hollow box structure with an open front side. The euthanasia box door 12 is installed on the front side of the euthanasia box 11. The euthanasia box door 12 is hinged or slidably connected to the front side of the euthanasia box 11 and can be opened and closed to facilitate the placement and removal of poultry. A sealing strip is provided on the inside of the euthanasia box door 12 to ensure that the euthanasia box 11 is sealed when the euthanasia box door 12 is closed.

[0046] The euthanasia chamber 11 has an air supply plate 13 installed on the top surface of its inner cavity, and several air supply nozzles 14 installed on the bottom surface of the air supply plate 13. Exhaust rods 15 are installed on both sides of the bottom surface of the inner cavity of the euthanasia chamber 11. The air supply plate 13 is connected to an external air source, and a specific gas, such as carbon dioxide or other euthanasia gas, is introduced into the air supply plate 13. The gas is then injected into the euthanasia chamber 11 through the air supply nozzles 14 below the air supply plate 13 to euthanize the poultry. The exhaust rods 15 are connected to an external negative pressure pump, which can create negative pressure to extract the gas. After the poultry is euthanized, the gas inside the euthanasia chamber 11 is extracted through the exhaust rods 15. This prevents the gas inside the euthanasia chamber 11 from causing harm to humans when the poultry is removed.

[0047] A poultry olfactory bulb sampling device includes a cutting component 3, which is installed on the top surface of the euthanasia component 1 and is used to precisely cut off the head of euthanized poultry.

[0048] The cutting assembly 3 includes a cutting box 31, a cutting box door 32, a lower door panel 33, a disinfection component 34, a suspension base 35, a suspension telescopic rod 36, a suspension component 37, and a cutting component 38. The euthanasia assembly 1 has a cutting box 31 on its top surface. The cutting box 31 is a hollow box structure with an open front side. A disinfection component 34 is installed on the top surface of the inner cavity of the cutting box 31 to disinfect the euthanized poultry and the cutting structure. A cutting box door 32 is installed on the upper part of the front side of the cutting box 31. The cutting box door 32 can be hinged or slidably connected to the front side of the cutting box 31 to achieve opening and closing, facilitating the placement and removal of the euthanized poultry. A lower door panel 33 is installed on the lower part of the front side of the cutting box 31. The lower door panel 33 is fixedly connected to the cutting box 31 to prevent blood and other substances from flowing out of the cutting box 31 during cutting. The bottom surface of the inner cavity of the cutting box is provided with a suspension base 35, and the top surface of the suspension base 35 is equipped with a suspension telescopic rod 36. The suspension telescopic rod 36 is an electric push rod, and those skilled in the art can choose the model themselves. The output end of the suspension telescopic rod 36 is equipped with a suspension component 37 to suspend the poultry for subsequent cutting operations. The upper part of the inner cavity of the cutting box is equipped with a cutting component 38 to cut the poultry and separate the head of the poultry.

[0049] The disinfection component 34 includes a disinfection plate 341, disinfection nozzles 342, a disinfection box 343, and a disinfection pump 344. The disinfection plate 341 is installed on the top surface of the inner cavity of the cutting box 31, and several disinfection nozzles 342 are arranged on the bottom surface of the disinfection plate 341. The disinfection box 343 is located on the top surface of the cutting box 31, and a disinfection pump 344 is installed on one side of the disinfection box 343. The input end of the disinfection pump 344 is connected to the disinfection box 343, and the output end of the disinfection pump 344 is connected to the disinfection plate 341 via pipes. The disinfection box 343 contains disinfectant solution. The disinfection pump 344 is a small liquid pump, and those skilled in the art can select the appropriate model. The disinfection pump 344 pumps the disinfectant solution from the disinfection box 343 into the disinfection plate 341, and then sprays it through the disinfection nozzles 342 into the interior of the cutting box 31, disinfecting the poultry's head, neck, and cutting structure. After disinfection, the poultry can be decapitated.

[0050] The suspension component 37 includes an upper suspension plate 3701, a lower suspension plate 3702, an intermediate plate 3703, a limiting motor 3704, and a limiting rod 3705. Both the upper suspension plate 3701 and the lower suspension plate 3702 have a U-shaped structure. The suspension telescopic rod 36 is an electric push rod; any model can be selected by those skilled in the art. The output end of the suspension telescopic rod 36 is provided with the intermediate plate 3703. The upper suspension plate 3701 and the lower suspension plate 3702 are respectively located on the upper and lower sides of the other end of the intermediate plate 3703. The U-shaped ends of the upper suspension plate 3701 and the lower suspension plate 3702 both face away from the intermediate plate 3703. The limiting motor 3704 is mounted on the bottom surface of the lower suspension plate 3702. The limiting motor 3704 is a stepper motor; any model can be selected by those skilled in the art. The output shaft of the limiting motor 3704 extends to the top surface of the lower suspension plate 3702 and is provided with the limiting rod 3705. When suspending poultry, the poultry's head is positioned above the U-shaped opening of the upper suspension plate 3701, with the poultry's neck inside the U-shape of the upper suspension plate 3701 and the lower suspension plate 3702, allowing the poultry's body to hang naturally. Finally, the limiting motor 3704 is activated, causing the limiting rod 3705 to rotate and turn towards the poultry's neck, thus restricting the poultry's neck to the inside of the upper suspension plate 3701 and the lower suspension plate 3702, preventing the poultry's suspension position from shifting.

[0051] The suspension component 37 further includes a lower extension rod 3706, an arc-shaped plate 3707, a suspension linear motor platform 3708, a fixed telescopic rod 3709, and a fixing plate 3710. The lower extension rod 3706 is located at the center of the bottom surface of the lower suspension plate 3702. Arc-shaped plates 3707 are located on both sides of the lower end of the lower extension rod 3706. The arc-shaped plates 3707 have a certain degree of toughness and are thin, arc-shaped plates. The suspended poultry's body rests between the two arc-shaped plates 3707. The suspension linear motor platform 3708 is an electric linear motor platform; any model can be selected by those skilled in the art. Suspension linear motor platforms 3708 are installed at the center of both sides of the inner cavity of the cutting box. Fixed telescopic rods 3709 are installed at the output ends of the suspension linear motor platforms 3708. The fixed telescopic rods 3709 are electric push rods; any model can be selected by those skilled in the art. A fixing plate 3710 is located at the output end of the fixed telescopic rod 3709. After the poultry is suspended, its body is located inside the two curved plates 3707. At this time, the suspension linear motor platform 3708 drives the fixed telescopic rod 3709 to move to both sides of the poultry's body. Then, the fixed telescopic rod 3709 drives the fixed plate 3710 to extend and press against the curved plate 3707, thereby fixing the poultry's body and preventing the poultry's body from shaking during the decapitation operation, which could lead to decapitation failure.

[0052] The cutting component 38 includes a cutting linear motor platform 381, a cutting cylinder 382, ​​a cutting blade 383, and a mating block 384. The cutting linear motor platform 381 is installed on the upper part of both sides of the inner cavity of the cutting box. The cutting linear motor platform 381 is an electric linear motor platform; those skilled in the art can choose the appropriate model. A cutting cylinder 382 is installed at the output end of each cutting linear motor platform 381. A cutting blade 383 is installed at the output end of one of the cutting cylinders 382, ​​and a mating block 384 is installed at the output end of the other cutting cylinder 382. The surface of the mating block 384 has a cutting groove adapted to the cutting blade 383. When both cutting linear motor platforms 381 are activated, they move the cutting cylinders 382 to both sides of the poultry's neck. The cutting cylinder 382 connected to the mating block 384 is activated first, moving the mating block 384 to one side of the poultry's neck. Then, the other cutting cylinder 382 is activated, causing the cutting blade 383 to strike the mating block 384, severing the poultry's neck between them. The shearing force is concentrated on the poultry's neck, achieving a neat and precise decapitation operation.

[0053] The two cutting cylinders 382, ​​one of which is a locking cylinder connected to the mating block 384, can extend the movable end a certain distance and fix it; the other is an impact cylinder connected to the cutting blade 383, which has a certain impact force when the movable end is extended. Those skilled in the art can choose the appropriate model of either cylinder to achieve decapitation of poultry necks.

[0054] A poultry olfactory bulb sampling device includes a dissection component 4, which is installed on the top surface of the euthanasia component 1. The dissection component 4 fixes the severed head of the poultry so that the experimenter can perform olfactory bulb dissection sampling.

[0055] The dissection assembly 4 includes a dissection frame 41, a leveling component 42, a rotating component 43, a dissection platform 44, and a fixing component 45. The top surface of the euthanasia assembly 1 is provided with a dissection frame 41. The leveling component 42 is installed in the middle of the bottom surface of the inner cavity of the dissection frame 41. The rotating component 43 is installed on the top surface of the leveling component 42. The output end of the rotating component 43 is installed with a dissection platform 44. The fixing component 45 is installed in the middle of the top surface of the dissection platform 44. The fixing component 45 fixes the poultry's head so that the olfactory bulb can be sampled during dissection.

[0056] The leveling component 42 includes leveling push rods 421 and leveling ball joint connectors 422. Four leveling push rods 421 are installed in the middle of the bottom surface of the inner cavity of the dissection frame 41. The leveling push rods 421 are electric push rods, which can be selected by those skilled in the art. The four leveling push rods 421 are arranged in a square to facilitate various posture adjustments of the dissection platform 44. The output end of the leveling push rod 421 is equipped with a leveling ball joint connector 422, which is a universal ball joint structure. The other end of the leveling ball joint connector 422 is fixedly connected to each corner of the top surface of the rotating component 43. By independently controlling the extension of the four leveling push rods 421, the angle compensation of the dissection platform 44 in any direction can be performed to achieve the angle adjustment of the dissection platform 44, which is convenient for researchers to perform olfactory bulb sampling operations after craniotomy of poultry heads.

[0057] The rotating component 43 includes a rotating box 431, a rotating box cover 432, a rotating motor 433, a supporting slide rail 434, a supporting slider 435, a fixing rod 436, a fixing tension spring 437, and a fixing magnet 438. The other ends of the four leveling ball joint connectors 422 are fixedly connected to the corners of the bottom surface of the rotating box 431. The rotating box 431 is a hollow box structure with an open front side. The rotating box cover 432 is installed on the front side of the rotating box 431. The rotating motor 433 is installed on the top surface of the inner cavity of the rotating box 431. The output end of the rotating motor 433 extends to the upper part of the top surface of the rotating box 431 and is provided with the dissection platform 44. The rotating motor 433 drives the dissection platform 44 to rotate, thereby adjusting the position of the poultry decapitation placed on the surface so that the experimenter can dissect and take samples from the olfactory bulb. The bottom surface of the dissection platform 44 is provided with a support slide rail 434, and the top surface of the rotating box is provided with a plurality of support sliders 435. The support sliders 435 are slidably connected to the support slide rail 434, thereby increasing the stability of the dissection platform 44.

[0058] A fixing rod 436 is provided on the top surface of the rotating box. A fixing groove is formed on the upper part of the fixing rod 436, and several fixing springs 437 are provided inside the fixing groove. A fixing magnet 438 is provided at the other end of each fixing spring 437. The fixing rod 436 is made of stainless steel and is not magnetically attracted. When the electromagnet is not energized, it retracts into the fixing groove of the fixing rod 436 under the tension of the fixing springs 437. When the electromagnet is energized, its outer end is attracted to the side of the dissection platform 44. At this time, half of the electromagnet is located outside the fixing groove and half is located inside the fixing groove, thus limiting the rotation of the dissection platform 44 and increasing the stability of the dissection platform 44 after it stops rotating.

[0059] The fixing component 45 is used to fix the severed head of poultry, fixing it from three parts: head, neck, and beak. The fixing component 45 includes a head fixing block 4501, a neck fixing block 4502, a beak fixing block 4503, a fixing frame 4504, a neck telescopic rod 4505, an inclined connecting seat 4506, a neck fixing plate 4507, a skin fixing plate 4508, a beak fixing plate 4509, a rotating seat 4510, a drive rod 4511, a drive push rod 4512, a drive seat 4513, a drive slider 4514, and a drive slide rail 4515.

[0060] A head fixing block 4501 is provided in the center of the top surface of the dissection platform 44. A head fixing groove is formed through the top surface of the head fixing block 4501. Skin fixing plates 4508, made of rubber, are respectively provided on both sides of the top surface of the head fixing block 4501, allowing for the insertion of pins. A neck fixing block 4502 and a beak fixing block 4503 are respectively provided on the rear and front sides of the head fixing block 4501. During placement, the poultry's head is placed in the head fixing groove of the head fixing block 4501, with the remaining neck resting on the surface of the neck fixing block 4502 and the beak resting on the surface of the beak fixing block 4503. During dissection, after the poultry's scalp is cut open, it is pulled apart to both sides, and fixing pins are inserted into the scalp to fix it to the surface of the skin fixing plates 4508 on both sides of the head fixing groove.

[0061] The dissection platform 44 has a mounting bracket 4504 on its top surface. A neck telescopic rod 4505 is mounted on the top of the mounting bracket 4504. The neck telescopic rod 4505 is an electric push rod, and its model can be selected by those skilled in the art. An inclined connecting seat 4506 is provided at the output end of the neck telescopic rod 4505. A neck fixing plate 4507 is provided at the bottom end of the inclined connecting seat 4506. The neck fixing plate 4507 has an arc-shaped structure and is inclined, with one side higher than the other. The neck fixing block 4502 is a wedge-shaped block, and the bottom surface of the neck fixing plate 4507 is approximately parallel to the surface of the neck fixing block 4502. The neck telescopic rod 4505 drives the neck fixing plate 4507 to press down, pressing the remaining neck of the decapitated poultry onto the surface of the neck fixing block 4502 for fixation.

[0062] A pair of rotating seats 4510 are provided on the top surface of the beak fixing block 4503. A beak fixing plate 4509 is rotatably connected between the two rotating seats 4510. A beak fixing groove is provided in the middle of the beak fixing plate 4509. A pair of driving rods 4511 are provided on the outer side of the beak fixing plate 4509. A driving slide rail 4515 is provided on the outer side of the driving rod 4511 along its length direction. A driving slider 4514 is slidably connected in the driving slide rail 4515. A driving push rod 4512 is installed on the top surface of the dissection platform 44. The driving push rod 4512 is an electric push rod, and its model can be selected by those skilled in the art. A driving seat 4513 is provided at the output end of the driving push rod 4512. The driving seat 4513 is rotatably connected to the driving slider 4514. When the drive push rod 4512 is activated, it drives the drive slider 4514 to move up and down through the drive seat 4513. Then, the drive rod 4511 drives the beak fixing plate 4509 to rotate around the rotating seat 4510, pressing it against the beak of the decapitated poultry. The beak fixing groove presses the decapitated beak of the poultry against the surface of the beak fixing block 4503 for fixation, thereby limiting the front side of the poultry's head.

[0063] A hanging basket containing dry ice and ice packs is installed on the bottom of the dissection platform to cool the platform above. The dissection platform is pre-cooled before separating the olfactory bulb.

[0064] After the poultry is decapitated and fixed, the skin is cut along the midsagittal line of the exposed part of the neck that is not fixed, and bluntly separated to both sides. The skin is then fixed to the surface of the skin fixation plate 4508 on both sides of the head fixation groove, thereby fully exposing the poultry's skull.

[0065] Using ophthalmic scissors, the bone of the anterior cranial fossa was cut near the upper margin of both orbits, and the bony connection in front of the olfactory bulb was removed. Then, a transverse incision was made on the dorsal side of the neck, and a small section of the suture was gently cut along the midsagittal suture of the skull. Subsequently, the skullcap was gently lifted with forceps to fully expose the brain tissue. The cranial nerves connected to the base of the brain were severed one by one, and the dura mater was carefully dissected to complete the exposure of the brain tissue.

[0066] Gently rinse the blood stains on the surface of the brain tissue with pre-cooled PBS phosphate-buffered saline, place it on a pre-cooled dissection platform 44, and use a scalpel to separate the olfactory bulb from the frontal lobe of the brain.

[0067] A timer and a rinsing device are installed on the side of the cutting box facing the dissection platform. Both are common knowledge in the art, and those skilled in the art can choose the appropriate model.

[0068] The timer is used to keep track of the dissection time during the dissection process.

[0069] The rinsing device has a built-in storage structure for storing physiological saline, and then uses a spray structure to rinse the dissected area during the dissection process to remove blood.

[0070] A matching back suction pipe is provided at the outer edge of the dissection platform. Several back suction holes are opened on the surface of the back suction pipe. The back suction pipe can back suction the wastewater generated by rinsing to avoid contaminating the operating environment.

[0071] The euthanasia component 1 has a placement component 5 installed on one side to place various experimental instruments used in the sampling operation.

[0072] The placement assembly 5 includes a placement top plate 51, a placement push rod 52, a placement slide 53, a placement motor 54, a rotating plate 55, a connecting plate 56, a placement frame 57, a placement box 58, and a placement magnet 59. The placement top plate 51 is located on the rear side of the cutting box 31. The placement push rod 52 is mounted on the bottom surface of the placement top plate 51. The placement push rod 52 is an electric push rod, and those skilled in the art can choose its model. The output end of the placement push rod 52 is provided with the placement slide 53. The placement push rod 52 drives the placement slide 53 to move up and down, thereby adjusting the position and height of the placement box 58 for the experimenter to access the experimental equipment. The placement motor 54 is mounted inside the placement slide 53. The placement motor 54 is a stepper motor, and its model can be selected by those skilled in the art. The output end of the placement motor 54 extends to the outside of the placement slide 53 and is provided with a rotating plate 55. The placement motor drives the rotating plate 55 to rotate a certain angle, thereby adjusting the position of the placement box 58. A connecting plate 56 is provided at the other end of the rotating plate 55. A placement frame 57 is rotatably connected to one side of the connecting plate 56. A placement box 58 is provided in the middle of the placement frame 57. Several placement magnets 59, which are electromagnets, are installed on the top surface of the connecting plate 56 near the placement frame 57. When the placement box 58 is not in use, the placement magnets 59 are de-energized, and the placement box 58 is in a vertical position. At this time, the placement motor 54 is activated, driving the placement box 58 to rotate to the rear of the cutting box 31 to avoid affecting the experimenters. When the placement box 58 is in use, the placement motor 54 is activated, driving the placement box 58 to rotate to the side of the dissection frame 41. Then, the placement box 58 is manually pulled up to make it horizontal. At this time, the placement magnets 59 are energized, attracting the placement frame 57 and fixing the placement frame 57 and the connecting plate 56 together. This fixes the placement frame 57 to a horizontal position, facilitating the placement of experimental instruments.

[0073] When using this invention:

[0074] 1. Perform euthanasia. Open the euthanasia chamber door, place the poultry inside, and close the door. Turn on the external air supply; euthanasia gas will be injected into the chamber through several nozzles on the bottom of the air supply plate to euthanize the poultry. After the poultry has died, start the negative pressure pump connected to the exhaust manifold to fully remove any remaining euthanasia gas from the chamber. Open the euthanasia chamber door and remove the poultry.

[0075] 2. Suspension and Body Fixation. Place the head of the euthanized poultry above the U-shaped opening of the upper suspension plate, with the neck inside the U-shape between the upper and lower suspension plates, allowing the body to hang naturally. The limit motor activates, rotating the limit rod towards the poultry's neck, thus confining the neck within the U-shape between the upper and lower suspension plates. At this point, the poultry's body is positioned between the two curved plates. The suspension linear motor platform activates, moving the fixed telescopic rod to an appropriate height on both sides of the poultry's body; subsequently, the fixed telescopic rod extends, pressing the fixed plate against the curved plates, clamping and fixing the poultry's body in place.

[0076] 3. Perform disinfection and decapitation. Close the cutting box door. Start the disinfection pump, drawing disinfectant solution from the box to the disinfection plate, which is then sprayed through the disinfection nozzles into the cutting box to disinfect the poultry's head, neck, and cutting structure. After disinfection, two linear cutting motor platforms start, driving the cutting cylinders to the predetermined cutting positions on both sides of the poultry's neck. The locking cutting cylinder connected to the mating block starts first, pushing the mating block out and fixing it to one side of the poultry's neck; then, the impact cutting cylinder connected to the cutting blade starts, driving the cutting blade to quickly impact the mating block. The cutting blade cuts into the cutting groove on the surface of the mating block, neatly severing the poultry's neck between the two, thus completing the decapitation operation.

[0077] 4. Fix the severed head and adjust its posture. Transfer the severed poultry head to the dissection platform. Place the head in the head fixation slot of the head fixation block, with the remaining neck and beak placed on the surface of the neck fixation block. Activate the neck extension rod, causing the neck fixation plate to press down and fix the remaining neck of the poultry head against the surface of the neck fixation block. Activate the drive push rod, causing the beak fixation slot in the middle of the beak fixation plate to press against the beak of the severed poultry head, fixing it against the surface of the beak fixation block.

[0078] By independently controlling the extension of four leveling push rods and coordinating with the universal adjustment of the leveling ball joint connector, the dissection platform can be angularly compensated in any direction to adjust to an angle convenient for the experimenter's operation. The rotating motor is then activated, causing the dissection platform to rotate horizontally to adjust the orientation of the severed head. At this point, the experimenter can dissect the severed head and collect olfactory bulb samples.

[0079] It should be noted that, in this document, relational terms such as “first” and “second” are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms “comprising,” “including,” or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus.

[0080] Other embodiments of this application will readily occur to those skilled in the art upon consideration of the specification and practice of the application disclosed herein. This application is intended to cover any variations, uses, or adaptations of this application that follow the general principles of this application and include common knowledge or customary techniques in the art not disclosed herein. The specification and embodiments are to be considered exemplary only, and the true scope and spirit of this application are indicated by the claims. It should be understood that this application is not limited to the precise structures described above and shown in the drawings, and various modifications and changes can be made without departing from its scope. The scope of this application is limited only by the appended claims.

Claims

1. A poultry olfactory bulb sampling device, characterized in that: Includes euthanasia components for euthanizing poultry; A cutting component is installed on the top surface of the euthanasia component. The adjustable cutting component can stably limit the movement of poultry of different sizes and then precisely control the cutting position of the head to avoid the deviation of manual cutting. The dissection component, installed on the top surface of the euthanasia component, achieves stable fixation and posture adjustment of poultry decapitation, ensuring no head movement during dissection. Operators can accurately locate the olfactory bulb tissue and perform dissection sampling, significantly ensuring sample integrity and effectively solving the problem of inconsistent sample quality during manual operation.

2. The poultry olfactory bulb sampling device according to claim 1, characterized in that: The euthanasia assembly includes an euthanasia chamber, an euthanasia chamber door, an air supply plate, air supply nozzles, and an exhaust rod. The euthanasia chamber is a hollow chamber structure with an open front side. An euthanasia chamber door is installed on the front side of the euthanasia chamber. An air supply plate is installed on the top surface of the inner cavity of the euthanasia chamber. Several air supply nozzles are installed on the bottom surface of the air supply plate. Exhaust rods are installed on both sides of the bottom surface of the inner cavity of the euthanasia chamber.

3. The poultry olfactory bulb sampling device according to claim 1, characterized in that: The cutting assembly includes a cutting box, a cutting box door, a lower door panel, a disinfection component, a suspension base, a suspension telescopic rod, a suspension component, and a cutting component. The cutting box is located on the top surface of the euthanasia assembly. The cutting box is a hollow box structure with an open front side. A disinfection component is installed on the top surface of the inner cavity of the cutting box. A cutting box door is installed on the upper part of the front side of the cutting box. A lower door panel is installed on the lower part of the front side of the cutting box. A suspension base is located on the bottom surface of the inner cavity of the cutting box. A suspension telescopic rod is installed on the top surface of the suspension base. A suspension component is installed at the output end of the suspension telescopic rod. A cutting component is installed on the upper part of the inner cavity of the cutting box. The cutting component cuts off the head of the poultry.

4. The poultry olfactory bulb sampling device according to claim 3, characterized in that: The disinfection component includes a disinfection plate, disinfection nozzles, a disinfection box, and a disinfection pump. The disinfection plate is installed on the top surface of the inner cavity of the cutting box, and several disinfection nozzles are provided on the bottom surface of the disinfection plate. The disinfection box is provided on the top surface of the cutting box, and a disinfection pump is installed on one side of the disinfection box. The input end of the disinfection pump is connected to the disinfection box, and the output end of the disinfection pump is connected to the disinfection plate through pipes.

5. The poultry olfactory bulb sampling device according to claim 3, characterized in that: The suspension system includes an upper suspension plate, a lower suspension plate, a middle plate, a limit motor, and a limit rod. Both the upper and lower suspension plates are U-shaped structures. The output end of the suspension telescopic rod is provided with a middle plate. The upper and lower suspension plates are respectively provided on the upper and lower sides of the other end of the middle plate. The U-shaped ends of the upper and lower suspension plates are both facing away from the middle plate. A limit motor is installed on the bottom surface of the lower suspension plate. The output shaft of the limit motor extends to the top surface of the lower suspension plate and is provided with a limit rod.

6. The poultry olfactory bulb sampling device according to claim 5, characterized in that: The suspension system also includes a lower extension rod, an arc-shaped plate, a suspended linear motor platform, a fixed telescopic rod, and a fixing plate. The lower extension rod is provided in the middle of the bottom surface of the lower suspension plate, and arc-shaped plates are provided on both sides of the lower end of the lower extension rod. Suspended linear motor platforms are installed in the middle of both sides of the inner cavity of the cutting box. A fixed telescopic rod is installed at the output end of the suspended linear motor platform, and a fixing plate is provided at the output end of the fixed telescopic rod.

7. The poultry olfactory bulb sampling device according to claim 3, characterized in that: The cutting component includes a linear cutting motor platform, a cutting cylinder, a cutting blade, and a mating block. The upper sides of the inner cavity of the cutting box are respectively equipped with linear cutting motor platforms. A cutting cylinder is installed at the output end of the linear cutting motor platform. A cutting blade is provided at the output end of one of the cutting cylinders, and a mating block is provided at the output end of the other cutting cylinder. A cutting groove adapted to the cutting blade is opened on the surface of the mating block.

8. The poultry olfactory bulb sampling device according to claim 1, characterized in that: Each corner of the bottom surface of the euthanasia component is equipped with a support leg.

9. A poultry olfactory bulb sampling device according to claim 1, characterized in that: The dissection assembly includes a dissection frame, a leveling component, a rotating component, a dissection platform, and a fixing component. The top surface of the euthanasia assembly is provided with a dissection frame. A leveling component is installed in the middle of the bottom surface of the inner cavity of the dissection frame. A rotating component is installed on the top surface of the leveling component. A dissection platform is installed at the output end of the rotating component. A fixing component is installed in the middle of the top surface of the dissection platform. The fixing component fixes the poultry's head so that the olfactory bulb can be sampled during dissection.

10. A poultry olfactory bulb sampling device according to claim 1, characterized in that: The euthanasia device has a placement component installed on one side.

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