Intelligent biogenic generator

Abstract

The application discloses an intelligent biological source generator and relates to the technical field of biological reinforcement of sewage treatment.The application innovatively constructs an integrated closed-loop technical system of monitoring-identification-propagation-cultivation-regulation, realizes the transformation and upgrading of biological reinforcement from passive response to active regulation, discards the passive mode of traditional biological reinforcement of first abnormality and then treatment, and through the collaborative integration of multiple modules, constructs a whole-process closed-loop system covering water quality-bacterial community monitoring, targeted bacterial community matching, on-site online propagation, precise dynamic addition and real-time feedback regulation, can predict the influence of water quality fluctuation on bacterial community in advance, initiatively starts targeted reinforcement measures, completely solves the technical bottleneck of monitoring lag and passive regulation of traditional technology, and greatly improves the operation stability of a biochemical treatment system.

Description

Technical Field

[0001] This invention relates to the field of wastewater treatment bioaugmentation technology, and in particular to an intelligent bio-generator. Background Technology

[0002] In the field of industrial wastewater treatment, biological treatment technology has become one of the most widely used mainstream technologies in water pollution control engineering due to its core advantages such as low energy consumption, low operating costs, excellent environmental compatibility, and no secondary pollution. As the core unit of deep wastewater purification, biochemical treatment systems rely on the metabolic activity of microbial communities to achieve the mineralization, transformation, and efficient removal of pollutants. Their operational stability and treatment efficiency directly determine whether wastewater can meet national and industry emission standards, playing a crucial strategic role in ensuring water environmental quality and safety and promoting the green and low-carbon development of the industrial sector.

[0003] With the increasing sophistication and complexity of industrial production processes, wastewater discharged from key industries such as coking, pharmaceuticals, pesticides, dyes, and coal chemicals exhibits typical characteristics of "high toxicity, high concentration, recalcitrant degradation, and complex composition." Specifically, this type of wastewater is rich in recalcitrant pollutants such as polycyclic aromatic hydrocarbons, heterocyclic compounds, nitrobenzenes, organophosphorus / chlorine compounds, and antibiotic residues, accompanied by extreme water quality conditions such as high ammonia nitrogen, high salinity, and strong acidity / alkalinity. The BOD5 / COD ratio of this type of wastewater is generally below 0.2, indicating extremely poor biodegradability. The indigenous microbial communities in conventional biological treatment processes such as activated sludge and biofilm methods are unable to tolerate the inhibitory effects of high concentrations of toxic pollutants and cannot degrade structurally stable characteristic pollutants. This leads to problems such as decreased microbial activity, biomass loss, and metabolic disorders, resulting in a significant decrease in the efficiency of the biological treatment system, effluent exceeding standards, and even system collapse, ultimately failing to meet the requirements for compliant industrial wastewater discharge.

[0004] To overcome the aforementioned technical bottlenecks, bioaugmentation technology has emerged. Its core principle is to add special microbial communities with specific metabolic functions (including resistant bacteria, halophilic bacteria, highly efficient nitrifying bacteria, denitrifying bacteria, heavy metal reducing bacteria, and characteristic pollutant degrading bacteria) to the biochemical treatment system to enhance the system's ability to degrade target pollutants and its resistance to shock loads, thereby improving the overall treatment efficiency. It is an important technical direction for solving the problem of treating recalcitrant industrial wastewater.

[0005] However, current traditional bioaugmentation technologies still have many technical shortcomings that urgently need to be addressed in engineering applications, which severely restrict the stability and reliability of their treatment effects and make it difficult to achieve large-scale promotion and application. The specific defects are as follows: First, there is a significant lag in the timing of microbial inoculation. Traditional bioaugmentation technology adopts a "passive response" inoculation mode, adding amplifying bacteria artificially only after the effluent quality of the biological treatment system deteriorates and the bacterial community becomes abnormal. This inoculation method cannot capture the impact of water quality fluctuations on bacterial community metabolism in real time. By the time the bacteria are added, the bacterial community structure in the system has already suffered irreversible damage, resulting in a significant reduction in the proliferation efficiency of the added bacteria, greatly diminishing the bioaugmentation effect, and failing to promptly curb the decline in system efficiency.

[0006] Secondly, the control of microbial inoculum dosage lacks scientific precision. In traditional bioaugmentation technology, the dosage mainly relies on the engineering experience of operators for judgment and adjustment, lacking scientific basis based on quantitative indicators such as water quality parameters and microbial activity. This results in insufficient precision in dosage control, easily leading to overdosing or underdosing. Overdosing not only significantly increases operating costs but may also cause secondary problems such as imbalance in microbial competition and damage to the ecological structure within the system. Underdosing, on the other hand, fails to effectively compensate for the functional deficiencies of the native microbial community, prolongs the system recovery period, and makes it difficult to ensure that the effluent water quality consistently meets standards.

[0007] Third, the activity of the microbial strains is difficult to guarantee effectively. Traditional bioaugmentation technologies often use freeze-dried or liquid microbial agents prepared in advance through large-scale factory cultivation. These strains require several weeks or even months to process during production, storage, transportation, and on-site storage. During this process, environmental factors such as temperature, oxygen, and nutrients can cause the strains to experience a gradual decline in activity and metabolic function. Even when added to a biological treatment system, they struggle to quickly adapt to the complex water environment and effectively perform their degradation function, further weakening the bioaugmentation effect.

[0008] In summary, traditional bioaugmentation technologies suffer from core technical challenges such as "monitoring lag, poor microbial compatibility, indiscriminate dosing, and insufficient microbial activity." The combined effect of these shortcomings makes it difficult to achieve the desired treatment results in industrial recalcitrant wastewater, limiting its large-scale promotion and application in this field. Therefore, developing a technology that can overcome these technical deficiencies and achieve precise, intelligent, and efficient bioaugmentation processes has become an urgent technical need in the current industrial wastewater treatment field. Summary of the Invention

[0009] The purpose of this invention is to address the core technical shortcomings of existing traditional bioaugmentation technologies in the treatment of recalcitrant industrial wastewater, such as "monitoring lag, poor microbial compatibility, blind addition, and insufficient microbial activity," as well as the resulting problems of unstable amplification effects, high operating costs, and difficulty in large-scale promotion. This invention provides an intelligent bio-generator that aims to break through the limitations of traditional technical frameworks, achieve precision, intelligence, and efficiency in the bioaugmentation process, and provide a brand-new technical equipment for the efficient biological treatment of recalcitrant industrial wastewater.

[0010] Specifically, the core objective of this invention is as follows: 1. To address the issue of delayed microbial inoculation timing in traditional bioaugmentation technologies, a real-time monitoring and rapid response mechanism is constructed to accurately capture and perceive fluctuations in the concentration of characteristic pollutants in wastewater, changes in water quality parameters, and the dynamic activity of microbial communities within the biochemical treatment system. This allows for timely initiation of targeted microbial community regulation, preventing irreversible damage to the microbial community structure and ensuring the timeliness of bioaugmentation.

[0011] 2. Overcoming the shortcomings of poor microbial community adaptability and insufficient targeting in traditional technologies, this technology uses a targeted identification and microbial community matching mechanism to accurately match microbial communities with specific degradation functions based on the type, concentration, and water quality conditions of characteristic pollutants. This achieves targeted adaptation between "pollutants and microbial communities" and improves the degradation efficiency of characteristic pollutants in recalcitrant industrial wastewater.

[0012] 3. Overcome the problems of traditional crude control of microbial inoculum dosage and lack of scientific basis, establish a data-driven dynamic dosage control system, based on real-time monitoring data and microbial community expansion progress, accurately adjust the amount and rate of inoculum dosage, avoid problems such as increased operating costs and microbial community imbalance caused by excessive or insufficient dosage, and ensure the stability of the ecological structure of the biochemical treatment system.

[0013] 4. Solve the problem of activity decay of traditional pre-prepared microbial strains during storage, transportation and storage. Through on-site online expansion technology, realize the real-time expansion and addition of specific microbial strains, fundamentally ensure the high activity and metabolic integrity of the added microbial strains, improve the adaptability of the microbial strains to the complex water quality environment on site, and ensure the stability and reliability of the bio-enhancement effect.

[0014] 5. Construct a fully closed-loop integrated system of "monitoring-control-dosing-feedback" to realize the transformation and upgrading of the bioaugmentation process from "experience-driven" to "data-driven", from "passive response" to "active control", and from "extensive dosing" to "precise matching". This will significantly improve the biochemical treatment system's resistance to shock loads and the degradation efficiency of characteristic pollutants in industrial recalcitrant wastewater, reduce operating costs, and ensure that the effluent quality consistently meets national and industry emission standards.

[0015] In summary, this invention aims to provide an integrated, intelligent, and efficient bio-generator by achieving the above-mentioned technical objectives. This will promote the upgrading and iteration of bio-enhancing technology in the field of industrial wastewater treatment, provide technical support for the efficient treatment of recalcitrant wastewater in key industries such as coking, pharmaceuticals, pesticides, dyes, and coal chemicals, and thus promote green, low-carbon, and sustainable development in the industrial sector. It has significant academic value and promising engineering applications.

[0016] To achieve the above-mentioned objectives, this invention provides an intelligent bio-source generator, which aims to address the core technological shortcomings of traditional bioaugmentation technology. This generator is a fully automated biological strain generation device that integrates "real-time monitoring, targeted identification, online amplification, dynamic matching, and continuous addition." By constructing an integrated system with multi-module collaborative operation and a closed-loop control system of "monitoring, regulation, addition, and feedback," the bioaugmentation process can be made more precise, intelligent, and efficient.

[0017] The specific technical solution of the present invention is as follows: The intelligent bio-generator mainly includes a real-time monitoring module, a targeted identification and microbial matching module, an online expansion and activity protection module, a dynamic addition and closed-loop control module, and an integrated rack for carrying each module and realizing signal and material transmission. Each module works together to complete the fully automated operation from water quality monitoring to precise addition of microbial strains.

[0018] I. Real-time monitoring module The real-time monitoring module serves as the "monitoring end" of the entire system. Its core function is to capture in real time the fluctuations in the concentration of characteristic pollutants in wastewater, changes in water quality parameters, and the dynamic activity of microbial communities within the biochemical treatment system. This enables precise perception of the synergistic changes in water quality and microbial communities, providing accurate and continuous data support for subsequent microbial community regulation, targeted matching, and dynamic dosing.

[0019] This module specifically includes a characteristic pollutant sensor group, an online water quality monitor, a microbial activity monitoring device, and a data acquisition and transmission unit. The configuration and functions of each component are as follows: 1. Characteristic Pollutant Sensor Assembly: This assembly is equipped with suitable specific sensors for characteristic pollutants in recalcitrant wastewater from industries such as coking, pharmaceuticals, pesticides, dyes, and coal chemicals. These sensors include polycyclic aromatic hydrocarbon sensors, nitrobenzene sensors, organophosphorus / chlorine sensors, antibiotic sensors, heterocyclic compound sensors, phenol sensors, total cyanide sensors, and thiocyanate sensors. The sensor probes are integrated into the biological generator, and the water intake is connected to the wastewater treatment structure (inlet end of the biological treatment tank, middle of the tank, and outlet end). The system monitors the concentration changes of the corresponding characteristic pollutants in real time, with a detection accuracy ≤0.1mg / L and a response time ≤30s.

[0020] 2. Online water quality monitoring instruments: including online COD monitors, online ammonia nitrogen monitors, salinity monitors, pH monitors, dissolved oxygen monitors, temperature monitors, and toxicity monitors, etc., to simultaneously monitor key water quality parameters of wastewater. The monitoring range for COD is 0-5000 mg / L, the monitoring range for ammonia nitrogen is 0 mg / L-200 mg / L, the monitoring range for salinity is 0 mg / L-30000 mg / L, and the monitoring range for pH is 1-14, ensuring coverage of the monitoring needs for extreme water quality conditions of recalcitrant industrial wastewater.

[0021] 3. Microbial activity monitoring equipment: A microbial respiration rate detector is used, along with an ATP detector, to monitor the activity status of both the native microbial community and the added microbial community within the biochemical treatment system.

[0022] 4. Data Acquisition and Transmission Unit: Adopting an industrial-grade data acquisition unit, it synchronously acquires monitoring data from various sensors and monitors via RS485 / Modbus communication protocol. After data preprocessing (filtering, noise reduction, calibration), it is transmitted to the target identification and microbial community matching module and the dynamic dosing and closed-loop control module via wired or wireless communication (5G / Ethernet). At the same time, it realizes real-time data storage and backtracking, with data transmission delay ≤1s.

[0023] II. Target Identification and Microbial Community Matching Module The targeted identification and microbial community matching module serves as the "core decision-making end" of the system. Its core function is to accurately identify the specific microbial communities required to degrade target pollutants based on the monitoring data transmitted by the real-time monitoring module, through big data algorithms and microbial metabolic mechanism models. This achieves targeted adaptation of "pollutants-microbial communities," solving the problems of poor microbial community adaptability and insufficient targeting in traditional technologies.

[0024] This module specifically includes an industrial-grade main control chip, a microbial community database, a targeted matching algorithm and model unit, and a decision command output unit. The configuration and functions of each component are as follows: 1. Industrial-grade main control chip: It adopts a high-performance embedded processor with a main frequency of no less than 2.0GHz, supports multi-threaded parallel processing, can quickly respond to real-time monitoring data and complete targeted matching calculations, and ensure the rapid generation of decision instructions.

[0025] 2. Microbial Community Database: A pre-built database of specific microbial communities adapted to the treatment of recalcitrant industrial wastewater is included. The database contains metabolic characteristics, compatible pollutant types, growth environment requirements, and community combination schemes of functional communities such as toxic bacteria, halophilic bacteria, highly efficient nitrifying bacteria, denitrifying bacteria, heavy metal reducing bacteria, polycyclic aromatic hydrocarbon degrading bacteria, organophosphorus degrading bacteria, azo dye degrading bacteria, thiocyanate degrading bacteria, phenol-resistant bacteria, and cyanide degrading bacteria. The database supports online updates and expansion, and can be supplemented with corresponding microbial community information according to newly added wastewater types and characteristic pollutants.

[0026] 3. Targeted Matching Algorithm and Model Unit: Integrating big data analysis algorithms and microbial metabolic mechanism models, the core algorithm is based on the types and concentrations of characteristic pollutants and water quality parameters (salinity, pH, temperature, etc.), combined with microbial community activity monitoring data, and through multi-factor coupling analysis, it screens out the most suitable specific microbial communities and microbial community combinations from the microbial community database, and calculates the optimal growth conditions (temperature, pH, dissolved oxygen, nutrient ratio, etc.) for the microbial community, forming targeted microbial community matching results and expansion parameter instructions.

[0027] 4. Decision Command Output Unit: Employing an industrial-grade programmable logic controller (PLC) and signal output module, the unit parses, compiles, and standardizes the target matching results and amplification parameter commands, converting them into standardized control signals recognizable by the system. These signals are then synchronously and in parallel transmitted to the online amplification and activity assurance module, as well as the dynamic addition and closed-loop control module. This ensures precise matching of the runtime sequence and parameter requirements of each functional module, enabling accurate linkage and coordinated operation between microbial community matching and subsequent amplification and addition processes. This eliminates command lag and control deviation, ensuring the fully automated and orderly operation of the entire system.

[0028] III. Online Propagation and Activity Assurance Module The online amplification and activity assurance module, as the "amplification end" of the system, has the core function of amplifying specific strains in real time on-site according to the instructions output by the target identification and microbial community matching module. By precisely controlling the amplification environment parameters, it ensures that the strains are always in a high-activity state, thus avoiding the problem of activity decay during the storage and transportation of traditional pre-prepared strains.

[0029] This module specifically includes a microbial seed bank, an online propagation reactor, an intelligent nutrient regulation unit, an environmental parameter regulation unit, and a propagation microbial activity detection unit. The settings and functions of each component are as follows: 1. Microbial Seed Bank: It adopts a sealed low-temperature storage tank (storage temperature is 4℃-8℃) with multiple independent storage chambers, which store preset specific microbial seed liquid or seed powder respectively.

[0030] 2. Online Propagation Reactor: Utilizing a batch bioreactor with an effective volume of 500L-1500L, it is equipped with aerobic / anaerobic / facultative anaerobic control functions based on the type of microbial community being propagated. The reactor's inner wall is coated to prevent adhesion, and an aeration and stirring device is installed at the bottom. The aeration intensity is adjustable within a range of 0.5m. 3 / (m 2 ·h)-5m 3 / (m 2 The stirring speed is adjustable from 20 r / min to 150 r / min to meet the propagation needs of different functional bacterial groups.

[0031] 3. Intelligent nutrient regulation unit: integrates a culture medium storage tank, a precision metering pump, and a nutrient ratio controller. The culture medium includes carbon sources (glucose, sodium acetate), nitrogen sources (urea, ammonium chloride), phosphorus sources (potassium dihydrogen phosphate), and trace elements (iron, manganese, zinc, etc.). Based on the growth requirements of the microbial community output by the targeted identification and microbial community matching module, the precision metering pump automatically adds each nutrient component with an addition accuracy of ≤0.1mL / min. The nutrient ratio adjustment range can meet the growth and metabolic needs of different microbial communities.

[0032] 4. Environmental parameter control unit: This includes a temperature control device, a pH control device, and a dissolved oxygen control device. The temperature control adopts a built-in heating / cooling system with a control range of 25℃-35℃ and a temperature control accuracy of ±2℃. The pH control is achieved by automatically adding acid (sulfuric acid) or alkali (sodium hydroxide) solution with a control accuracy of ±0.1%. The dissolved oxygen control is achieved through the coordinated action of an aeration device and a stirring device, with a control range of 0.1mg / L-5mg / L, ensuring that the expansion culture environment parameters are precisely matched with the growth requirements of the microbial community.

[0033] 5. Propagation Bacterial Activity Detection Unit: Equipped with a portable microbial activity detector, it monitors the activity and concentration changes of the microorganisms in the online propagation reactor in real time. When the microbial concentration reaches 10... 9 When the CFU / mL concentration and activity are ≥90%, a signal indicating that the strain meets the standard is generated and transmitted to the dynamic addition and closed-loop control module, triggering the addition command. If the activity is below 70%, the nutrient ratio and environmental parameters are automatically adjusted to ensure that the strain is in a high-activity state before addition.

[0034] IV. Dynamic Dosing and Closed-Loop Control Module The dynamic dosing and closed-loop control module serves as the "dosing end" and "control core" of the system. Its core function is to precisely adjust the dosage and dosing rate of the bacteria through an automated control system based on real-time monitoring data, target matching results, and the achievement of standards for the cultured bacteria. This achieves dynamic adaptation between the dosage of specific bacteria and changes in pollutant concentration. At the same time, it receives feedback data on effluent water quality and bacterial activity, optimizes control parameters in real time, and forms a complete closed-loop control.

[0035] This module specifically includes an automated dosing device, a flow metering unit, a closed-loop control controller, and a feedback adjustment unit. The configuration and functions of each component are as follows: 1. Automated dosing device: It adopts a variable frequency metering pump and is equipped with a multi-channel dosing pipeline. Each channel corresponds to the dosing path of a specific bacterial group. The dosing pipeline is connected to different areas of the biological tank (water inlet end, middle of the tank body) to ensure uniform diffusion of the bacterial species. The dosing rate of the variable frequency metering pump is adjustable from 10L / h to 500L / h, with an adjustment accuracy of ≤1L / h. It can achieve continuous or intermittent dosing according to the control command.

[0036] 2. Flow metering unit: Electromagnetic flow meters are installed in each dosing pipeline and at the inlet of the biological treatment tank to monitor the dosing flow rate of the bacteria and the inlet flow rate of the biological treatment tank in real time. The dosing flow rate monitoring accuracy is ±0.5%, and the outlet flow rate monitoring range is 0 m³ / d-5000 m³ / d, providing flow data support for precise control of the dosing amount.

[0037] 3. Closed-loop control controller: A PLC controller with a built-in dynamic dosing control model is adopted. This model is based on the characteristic pollutant concentration, water quality parameters and bacterial activity data transmitted by the real-time monitoring module, combined with the concentration of cultured bacteria, and calculates the optimal dosage and dosing rate through algorithms. It generates control commands and sends them to the automated dosing device to achieve dynamic adaptation of "increased pollutant concentration → increased dosage, decreased pollutant concentration → decreased dosage". At the same time, the controller can store multiple dosing schemes to adapt to the wastewater treatment needs of different industries.

[0038] 4. Feedback Adjustment Unit: Equipped with a multi-parameter online water quality monitor and an intelligent feedback controller, it collects real-time water quality monitoring data such as the concentration of characteristic pollutants, COD, and ammonia nitrogen at the effluent of the biological treatment tank, as well as system microbial activity data. The real-time monitoring data is compared and analyzed in real time with the equipment's preset emission standards and microbial activity thresholds. If the effluent water quality exceeds the standard or the microbial activity is abnormal, the intelligent feedback controller will automatically generate and trigger dynamic adjustment commands, iteratively optimize the targeted microbial matching scheme, online expansion operation parameters, and microbial dosage, and continuously correct the system's operating status until the effluent water quality meets the standard and the microbial activity returns to stability. This constructs a complete closed-loop control system of "concentration monitoring → microbial regulation → precise addition → effect feedback → parameter optimization," effectively offsetting interference factors such as water quality load fluctuations and microbial activity decay, ensuring the long-term stability and treatment reliability of the system.

[0039] V. Integrated rack for carrying various modules and realizing signal and material transmission The integrated frame is made of corrosion-resistant carbon steel or stainless steel, and has anti-corrosion, dustproof and waterproof functions, making it suitable for the harsh environment of industrial wastewater treatment sites. Each module is equipped with an independent fault alarm unit. When a component fails (sensor malfunction, metering pump failure, data transmission interruption, etc.), it automatically issues an audible and visual alarm and records the fault information. At the same time, it starts the backup components (backup sensor, backup metering pump) to ensure continuous system operation and reduce the impact of faults on the treatment effect.

[0040] VI. Collaborative Operation Mechanism of Each Module The various modules of the intelligent bio-generator of this invention operate in concert to achieve fully automated control of the entire process. The specific operation process is as follows: 1. Start-up phase: After the system is powered on, the real-time monitoring module starts first, and all sensors and monitors begin to work, collecting initial data on wastewater characteristic pollutant concentrations, water quality parameters, and bacterial activity, and transmitting them to the target identification and bacterial matching module; 2. Targeted Matching Stage: Based on the initial monitoring data, the targeted identification and microbial community matching module uses a model to screen out suitable specific microbial communities, generates microbial community matching results and expansion parameter instructions, and transmits them to the online expansion and activity assurance module; 3. Online propagation stage: The online propagation and activity assurance module extracts the corresponding bacterial seed liquid or seed powder from the bacterial seed bank according to the instructions, automatically adds culture medium and adjusts parameters such as temperature, pH, and dissolved oxygen to start online propagation, while monitoring the bacterial activity and concentration in real time. 4. Dynamic addition stage: When the concentration and activity of the expanded strains reach the standard, the online expansion and activity assurance module sends a standard-reaching signal to the dynamic addition and closed-loop control module. This module calculates the optimal addition amount based on real-time monitoring data and starts the automated addition device to accurately add the highly active specific bacteria to the biochemical treatment system. 5. Closed-loop control stage: The dynamic dosing and closed-loop control module receives real-time feedback data on effluent water quality and bacterial activity, compares it with preset thresholds, and dynamically adjusts the dosing amount, culture parameters, and bacterial matching scheme to ensure stable performance of the biochemical treatment system and continuous compliance of effluent water quality.

[0041] The beneficial effects of this invention are as follows: I. Technical Aspect: Breaking through traditional technical bottlenecks to achieve precise and intelligent upgrades in bio-enhancement. 1. Solving the problem of monitoring lag and improving the timeliness of system response: This invention integrates high-precision characteristic pollutant sensors, online water quality monitors, and microbial activity monitoring equipment through a real-time monitoring module. This enables real-time capture and accurate perception of the concentration of characteristic pollutants in wastewater, key water quality parameters, and the dynamics of microbial activity. Compared with the traditional "passive response" dosing mode, it can predict the impact of water quality fluctuations on microbial metabolism in advance, promptly initiate targeted microbial regulation, avoid irreversible damage to the microbial structure, significantly improve the timeliness and effectiveness of bioaugmentation, and ensure the stable operation of the biochemical treatment system.

[0042] 2. Optimizing microbial community adaptability and improving the degradation efficiency of characteristic pollutants: This invention relies on a targeted identification and microbial community matching module, combined with big data algorithms and microbial metabolic mechanism models, to achieve targeted adaptation of "pollutants-microbial communities". It can accurately screen suitable specific functional microbial communities for characteristic pollutants in wastewater from different industries such as coking, pharmaceuticals, and pesticides. Compared with the universal microbial strain addition method in traditional technologies, it significantly improves the targeted degradation of recalcitrant pollutants, effectively breaks through the technical bottleneck of poor biodegradability of industrial recalcitrant wastewater, and greatly improves the removal rate of characteristic pollutants and the treatment efficiency of the biochemical treatment system.

[0043] 3. Achieving precise dosing and control to ensure stable microbial community ecology: This invention, through a dynamic dosing and closed-loop control module, constructs a data-driven dosing control model based on real-time monitoring data. It precisely adjusts the amount and rate of microbial dosing, achieving dynamic adaptation between the dosing amount and pollutant concentration changes. Compared with the traditional extensive dosing mode that relies on manual experience, it completely solves the problem of overdosing or underdosing. It avoids the imbalance of microbial community competition and destruction of ecological structure caused by overdosing, and eliminates the prolonged recovery cycle of the biochemical treatment system caused by underdosing, thus ensuring the stability of the microbial community ecological structure within the biochemical treatment system.

[0044] 4. Ensuring high bacterial activity and improving environmental adaptability: This invention enables real-time on-site cultivation of specific bacterial strains through an online expansion and activity assurance module, eliminating the need for pre-storage and transportation of pre-prepared bacterial strains. This fundamentally avoids the activity degradation problem that occurs during the storage and transportation of traditional freeze-dried bacterial agents or liquid bacterial solutions. At the same time, by precisely controlling the expansion environment parameters (temperature, pH, dissolved oxygen, nutrient ratio, etc.), it ensures that the added bacterial strains are always in a high-activity state, significantly improving the adaptability of the bacterial strains to extreme water quality conditions (high salinity, high toxicity, strong acidity and alkali) of industrial wastewater, and ensuring the stability and reliability of the bio-enhancement effect.

[0045] 5. Constructing a closed-loop control system to improve system stability: This invention constructs a complete closed-loop control system through multi-module collaboration, encompassing "concentration monitoring → microbial community regulation → precise dosing → effect feedback → parameter optimization." This enables the bioaugmentation process to transform from "experience-driven" to "data-driven," and from "passive response" to "active regulation." It can optimize control parameters in real time based on effluent quality and microbial activity feedback data, effectively resisting the shock load caused by fluctuations in industrial wastewater quality. This significantly improves the operational stability and anti-interference capability of the biochemical treatment system, ensuring that the effluent quality consistently meets standards.

[0046] II. Economic Aspect: Reduce operating costs and improve the economic efficiency of engineering applications 1. Reduced cost of using microbial strains: This invention adopts an on-site online propagation mode, eliminating the need for purchasing, storing, and transporting pre-made microbial strains, which significantly saves on the costs associated with the production, packaging, transportation, and storage of microbial strains. At the same time, the precise dosing mode avoids waste caused by excessive dosing of microbial strains, further reducing the cost of using microbial strains.

[0047] 2. Reduced labor and maintenance costs: This invention is a fully automated operating device that can achieve unmanned operation of the entire process from water quality monitoring, microbial community matching, online propagation to dynamic dosing. Compared with the traditional bioaugmentation technology, which requires manual monitoring, manual dosing, and manual adjustment, this invention significantly reduces the number of operators and labor intensity, thereby reducing labor costs. At the same time, the device is equipped with fault alarms and backup components, which can promptly detect and handle operational faults, reducing the difficulty and cost of operation and maintenance.

[0048] 3. Reduce subsequent treatment costs: By improving the degradation efficiency of the biochemical treatment system for characteristic pollutants, this invention can significantly reduce the treatment load of subsequent advanced treatment units, reduce the amount of advanced treatment agents, energy consumption and sludge production, thereby reducing subsequent treatment costs and improving the overall economic efficiency of industrial wastewater treatment projects.

[0049] III. Environmental Protection and Industry: Promoting Technological Upgrading and Supporting Green and Low-Carbon Development 1. Ensure wastewater meets discharge standards and reduce environmental risks: This invention can effectively improve the treatment efficiency of recalcitrant wastewater from key industries such as coking, pharmaceuticals, pesticides, dyes, and coal chemicals, ensuring that the effluent quality consistently meets national and industry discharge standards, significantly reducing the discharge of toxic and harmful pollutants in wastewater, reducing the risk of water pollution, and ensuring the safety of water environment quality.

[0050] 2. Promoting the upgrading of wastewater treatment technology: As an innovative bio-augmentation technology and equipment, this invention integrates technologies from multiple fields such as real-time monitoring, big data analysis, microbial engineering and automated control, realizing the intelligent and precise upgrading of bio-augmentation technology, providing a new technical path for the treatment of industrial recalcitrant wastewater, and promoting the technological iteration and progress in the field of industrial wastewater treatment.

[0051] 3. Supporting Green and Low-Carbon Development of Industries: This invention leverages the advantages of biological treatment technology to further improve the energy efficiency of wastewater treatment, reducing energy consumption and secondary pollution during the treatment process. At the same time, it provides reliable technical support for the treatment of wastewater in key polluting industries to meet standards, helping related industries achieve clean production and green development, promoting the green and low-carbon transformation of the industrial sector, and has significant industrial promotion value.

[0052] In summary, the intelligent bio-generator of this invention breaks through the core bottleneck of traditional bio-enhancing technology, has significant cost advantages, and has important promotional value in environmental protection and industry. Its application can effectively improve the treatment efficiency of industrial recalcitrant wastewater, reduce treatment costs, and ensure environmental safety. It is of great significance for promoting technological upgrading in the field of industrial wastewater treatment, improving water pollution control, and promoting green and sustainable industrial development, and has broad engineering application prospects. Attached Figure Description

[0053] Figure 1 A block diagram of an intelligent bio-generator structure is shown. Detailed Implementation

[0054] To further illustrate the technical solution, implementation process, and application effects of this invention, the following uses the treatment of recalcitrant wastewater from the coking industry as an example, combining specific device configuration, operating parameters, and treatment data to detail the implementation method of this intelligent bio-generator. This embodiment is only used to explain this invention and is not intended to limit the scope of protection of this invention. Its core principles and technical solutions are also applicable to the bio-enhanced treatment of recalcitrant wastewater from other industries such as pharmaceuticals, pesticides, dyes, and coal chemicals.

[0055] I. Overview of Implementation Scenarios This embodiment selects a two-stage AO biochemical treatment system in a coking plant, with a total effective volume of 1200 m³. 3 It is divided into a first-level AO unit (800m) 3 ) and secondary AO unit (400m 3 ), with a 200m² secondary sedimentation tank 3 It mainly treats process wastewater generated during coking production. The influent water quality indicators are as follows: COD = 3000mg / L-3800mg / L, ammonia nitrogen (NH3-N) = 100mg / L-200mg / L, phenolic compounds = 500mg / L-600mg / L, total cyanide (CN) = 100mg / L-2 ... - ) = 20mg / L-40mg / L, thiocyanate (SCN) - () = 600mg / L-800mg / L, pH=8.5-9.5, BOD5 / COD=0.18, extremely poor biodegradability, and contains trace amounts of polycyclic aromatic hydrocarbons, heterocyclic compounds and other recalcitrant pollutants.

[0056] The original bioaugmentation scheme for this system involved the periodic manual addition of pre-prepared microbial agents (twice a month, with a single addition of 50 kg based on experience, and the nominal activity of the agents being ≥90%). However, in actual operation, three major problems arose: First, the activity of the microorganisms was insufficient. After storage and transportation, the activity of the pre-prepared microbial agents decreased to 55%-65%, making it difficult for them to quickly adapt to high cyanide, high thiocyanate, and alkaline environments after addition. Second, the addition was done blindly, without quantitative indicators to support it. The frequency of addition was adjusted only based on fluctuations in the effluent COD, which easily led to over-addition causing an imbalance in microbial competition, or under-addition failing to compensate for functional defects. Third, monitoring was lagging. Only daily manual sampling and testing of key indicators of influent and effluent were conducted, which could not capture the impact of water quality fluctuations on microbial metabolism in real time. By the time the effluent exceeded the standards, the microbial structure of the system had already suffered irreversible damage. The aforementioned problems resulted in unstable effluent quality from the original system. During normal operation, COD was 400-500 mg / L, ammonia nitrogen was 35-45 mg / L, phenols were 25-40 mg / L, total cyanide was 5-8 mg / L, and thiocyanate was 120-180 mg / L, failing to meet the emission requirements of Table 2 of the "Emission Standard of Pollutants for Coking Chemical Industry" (GB 16171-2012) (COD ≤ 80 mg / L, ammonia nitrogen ≤ 15 mg / L, phenols ≤ 0.5 mg / L, total cyanide ≤ 0.5 mg / L, thiocyanate ≤ 10 mg / L). To address this bottleneck, the intelligent bio-generator of this invention was used for bio-enhancement modification.

[0057] II. Configuration Parameters of Intelligent Biogenerator The intelligent bio-generator in this embodiment adopts a modular integrated design, is made of stainless steel, and occupies an area of ​​20m². 2 It is adapted to industrial site conditions and can directly interface with two-level AO systems, such as... Figure 1 As shown, the core includes a real-time monitoring module, a target identification and microbial community matching module, an online amplification and activity protection module, and a dynamic dosing and closed-loop control module. Specific configuration parameters are as follows: (a) Configuration of real-time monitoring module 1. Characteristic pollutant sensor group: Equipped with a phenol sensor (detection range 0mg / L-2000mg / L, accuracy ±1mg / L), a total cyanide sensor (detection range 0mg / L-100mg / L, accuracy ±0.1mg / L), and a thiocyanate sensor (detection range 0mg / L-2000mg / L, accuracy ±2mg / L), and simultaneously adapted to a rapid polycyclic aromatic hydrocarbon detector (detection range 0mg / L-50mg / L, accuracy ±0.5mg / L) to specifically capture the concentration fluctuations of characteristic pollutants in coking wastewater.

[0058] 2. Water Quality Online Monitoring Instrument: Integrates an online COD monitor (measurement range 0mg / L-5000mg / L, error ±2%FS), an online ammonia nitrogen monitor (measurement range 0mg / L-500mg / L, error ±0.1mg / L), a pH monitor (measurement range 0-14, accuracy ±0.01), a dissolved oxygen (DO) monitor (measurement range 0mg / L-20mg / L, accuracy ±0.1mg / L), a temperature monitor (measurement range 0℃-100℃, accuracy ±0.1℃), and a toxicity monitor (detection range 0TU-100TU, accuracy ±0.5TU), to collect key water quality parameters in real time.

[0059] 3. Microbial activity monitoring equipment: A microbial respiration rate detector is used, with a detection range of 0 mgO2 / (L·h)-50 mgO2 / (L·h) and an accuracy of ±0.1 mgO2 / (L·h). It is simultaneously equipped with an ATP detector (detection range of 0 RLU-1000 RLU and accuracy of ±5 RLU) to dual monitor the activity status of the indigenous microbial community and the added microbial community in the biochemical treatment system.

[0060] 4. Data Acquisition and Transmission Unit: Equipped with an industrial-grade PLC data acquisition unit, it supports multi-channel signal access, data transmission delay ≤500ms, data accuracy ≥99%, and transmits data to the core control module via Ethernet + 4G dual links to ensure data continuity.

[0061] (II) Configuration of Target Identification and Microbial Community Matching Module 1. Microbial community database: Built-in information on functional microbial communities such as phenol-resistant bacteria (Pseudomonas, Bacillus), cyanide-degrading bacteria (Alcaligenes, Arthrobacter), thiocyanate-degrading bacteria (Thiobacillus, Micrococcus), highly efficient nitrifying bacteria (Nitrosomonas, Nitrobacter), and denitrifying bacteria (Paracoccus, Pseudomonas), covering core parameters such as metabolic characteristics, suitable pollutant types, optimal growth pH, ​​temperature, and dissolved oxygen range for each microbial community, and supporting dynamic updates of microbial community information.

[0062] 2. Targeted matching algorithm and model unit: Integrating multi-factor coupling analysis algorithm and microbial metabolic mechanism model, based on real-time monitoring of characteristic pollutant concentrations and water quality parameters (pH, temperature, toxicity, etc.), it quickly calculates the optimal bacterial community combination and suitable amplification parameters. The time taken for a single matching is ≤10s, ensuring timely regulation.

[0063] (III) Configuration of Online Propagation and Activity Assurance Module 1. Microbial seed bank (50L): uses a sealed low-temperature storage tank.

[0064] 2. Online expansion reactor: It adopts a batch bioreactor with an aerobic expansion chamber (1500L), which supports the simultaneous expansion of single and complex bacterial groups. The chamber is equipped with an anti-corrosion coating and is suitable for expansion conditions of alkaline and cyanide-containing wastewater.

[0065] 3. Intelligent nutrient regulation unit: Integrates an intelligent culture medium dosing system (dosing accuracy ±0.1mL), which can precisely add carbon source (glucose), nitrogen source (ammonium chloride), phosphorus source (potassium dihydrogen phosphate), and trace elements (Fe) according to the needs of the microbial community. 3+ Mn 2 + ).

[0066] 4. Environmental parameter control unit: including temperature control device (control range 25℃-35℃, accuracy ±2℃), pH control device (control range 6-10, accuracy ±0.1), and dissolved oxygen control device (control range 0.1mg / L-5mg / L, accuracy ±0.2mg / L), which can adaptively adjust environmental parameters according to the growth needs of different bacterial communities.

[0067] 5. Spectrosporin Activity Detection Unit: Equipped with a bacterial community concentration detector (detection range 10). 6 -10 12 (CFU / mL, accuracy ±5%) and ATP activity detector to monitor the status of the cultured microbial community in real time. When the microbial community concentration is ≥1.0×10⁻⁶, the detection rate is determined by the detection method. 9 A dosing readiness signal is triggered when CFU / mL and activity ≥90%; equipped with a 0.22μm precision filtration system and an ultraviolet disinfection device (30W power) to control the contamination rate of miscellaneous bacteria to ≤0.1%.

[0068] 6. Anti-toxic adaptation unit: In response to the characteristics of high cyanide and high thiocyanate, a gradient acclimatization component is added to the expansion chamber to gradually improve the tolerance of the microbial community to toxic pollutants.

[0069] (iv) Configuration of dynamic dosing and closed-loop control module 1. Automated dosing unit: Equipped with a 4-channel variable frequency metering pump (single pump dosing range 0.1L / h-100L / h, accuracy ±1%), the dosing pipeline is connected to the four functional tanks of the two-stage AO system (primary anaerobic tank, primary aerobic tank, secondary anaerobic tank, and secondary aerobic tank), supporting precise dosing at multiple points.

[0070] 2. Flow metering unit: Electromagnetic flow meters are installed in each dosing pipeline and at the inlet of the biological treatment tank to monitor the dosing flow rate of the bacteria and the inlet flow rate of the biological treatment tank in real time.

[0071] 3. Closed-loop control controller: It adopts a PLC core controller and is equipped with a dynamic dosing control model. Based on the data (concentration of characteristic pollutants, bacterial activity, water quality parameters) and the propagation progress transmitted by the real-time monitoring module, it automatically calculates the optimal dosage and dosing rate to achieve dynamic adaptation of dosing parameters.

[0072] 4. Feedback and Regulation Unit: Receives real-time water quality monitoring data (concentration of characteristic pollutants, COD, ammonia nitrogen, etc.) and bacterial activity data from the effluent of the biological treatment tank. Compares this data with preset discharge standards and bacterial activity thresholds. If the effluent quality exceeds the standards or bacterial activity is abnormal, it automatically triggers regulation commands to adjust the targeted bacterial matching scheme, propagation parameters, and dosage until the effluent quality meets the standards and bacterial activity stabilizes. This forms a complete closed-loop control system of "concentration monitoring → bacterial regulation → precise dosage → effect feedback → parameter optimization," ensuring the stability and reliability of the system operation.

[0073] 5. Human-machine interaction and early warning unit: Equipped with a 10-inch industrial touch screen, it supports parameter setting, data query, status monitoring, and fault diagnosis; preset thresholds for each indicator, and promptly issue audible and visual alarms and record fault information when water quality or bacterial activity is abnormal; supports manual / automatic mode switching to improve operational flexibility.

[0074] III. Implementation Procedures In this embodiment, the intelligent bio-generator and the two-stage AO system operate in coordination, following a complete process of "start-up docking - real-time monitoring - target matching - online expansion culture - dynamic addition - closed-loop control". The specific operation steps are as follows: 1. Device Start-up and System Integration: Fix the intelligent bio-generator next to the coking plant's biochemical treatment system, connect it to a 380V industrial power supply, connect the sampling pipeline to the inlet of the two-stage AO system and each functional tank, and connect the dosing pipeline to the primary anaerobic tank, primary aerobic tank, secondary anaerobic tank, and secondary aerobic tank respectively. Complete pipeline sealing and leak detection. Initialize parameters through the human-machine interface, preset the effluent control targets (COD≤80mg / L, ammonia nitrogen≤15mg / L, phenols≤0.5mg / L, total cyanide≤0.5mg / L, thiocyanate≤10mg / L), bacterial activity≥90%, and amplified bacterial concentration≥1.0×10⁻⁶. 9 CFU / mL.

[0075] 2. Real-time monitoring and data acquisition: The real-time monitoring module is activated to continuously collect characteristic pollutant concentrations (phenols, total cyanide, thiocyanate), water quality parameters (COD, ammonia nitrogen, pH, DO, temperature, toxicity), and bacterial activity data of the influent and each functional tank of the two-stage AO system. A set of data is updated every 30 seconds and transmitted synchronously to the targeted identification and bacterial matching module and the dynamic dosing and closed-loop control module after noise reduction, forming a dynamic database of water quality and bacterial community.

[0076] 3. Targeted Microbial Community Matching and Parameter Output: Based on monitoring data (COD=3800mg / L, ammonia nitrogen=200mg / L, phenols=600mg / L, total cyanide=40mg / L, thiocyanate=800mg / L, pH=9.0), the targeted identification and microbial community matching module uses a multi-factor coupling analysis algorithm and a microbial metabolic mechanism model to accurately match a complex microbial community combination: phenol-resistant bacteria (Pseudomonas spp.) + cyanide-degrading bacteria (Alcaligenes spp.) + thiocyanate-degrading bacteria (Thiobacillus spp.) + highly efficient nitrifying bacteria (Nitrosomonas spp. + Nitrobacillus spp.) + denitrifying bacteria (Paracoccus spp.). It outputs targeted expansion parameters: temperature 28℃-32℃, pH 7.8-8.5, DO=2.5mg / L-4.0mg / L during aerobic expansion, and culture medium ratio (carbon source: nitrogen source: phosphorus source=100:6:1.2), with simultaneous addition of Fe. 3+ Mn 2+ Trace elements, suitable for alkaline and highly toxic environments.

[0077] 4. On-site Online Propagation and Activity Control: The online propagation and activity assurance module receives the microbial community matching results and propagation parameter instructions, activates the microbial seed bank, and introduces the compound microbial community seeds into the aerobic propagation chamber. Nutrients are precisely added through an intelligent culture medium dosing system, and temperature, pH, and DO control units are activated simultaneously to maintain stable propagation environment parameters. The microbial community is then subjected to gradient acclimatization using an anti-toxic adaptation unit to gradually improve its tolerance to total cyanide and thiocyanate. Real-time monitoring of microbial community concentration and activity is conducted, and the activity is monitored when the microbial community concentration reaches 1.2 × 10⁻⁶. 9 When the CFU / mL concentration and activity reach 92%, a dosing readiness signal is triggered and synchronously transmitted to the dynamic dosing and closed-loop control module.

[0078] 5. Dynamic and Precise Dosing: The dynamic dosing and closed-loop control module calculates the optimal dosing amount and dosing rate at each point based on real-time monitoring data and the achievement of standards for the expanded microbial community through a control model: In the primary anaerobic tank, phenol-resistant bacteria + cyanide-degrading bacteria + thiocyanate-degrading bacteria are added (dosage 12L / h-15L / h) to enhance the pretreatment and toxicity reduction of recalcitrant pollutants; in the primary aerobic tank, highly efficient nitrifying bacteria are added (dosage 8L / h-10L / h) to improve ammonia nitrogen degradation efficiency; in the secondary anaerobic tank, denitrifying bacteria are added (dosage 6-8L / h) to enhance nitrogen removal; and in the secondary aerobic tank, highly efficient nitrifying bacteria + phenol-resistant bacteria are added (dosage 10L / h-12L / h) to deeply degrade residual pollutants, achieving dynamic adaptation between microbial community dosing and pollutant concentration.

[0079] 6. Closed-Loop Feedback Control: The real-time monitoring module continuously tracks water quality changes and bacterial activity in each functional tank of the two-stage AO system after dosing, transmitting feedback data to the closed-loop control module. If the ammonia nitrogen concentration in the primary aerobic tank is >30mg / L, the dosage of high-efficiency nitrifying bacteria is automatically increased to 10L / h-12L / h, while the expansion temperature is adjusted to 30℃-32℃ to optimize nitrification efficiency. If the total cyanide concentration is >5mg / L, the dosage of cyanide-degrading bacteria is increased, and the nutrient ratio in the expansion chamber is adjusted simultaneously to enhance the bacterial community's resistance to toxic metabolism. If the bacterial activity is <85%, the culture medium is automatically replenished and environmental parameters are adjusted to ensure stable bacterial function. Through continuous feedback optimization, a complete closed loop of "monitoring-matching-expansion-dosing-control" is formed, ensuring that the effluent water quality meets standards.

[0080] 7. Daily Operation and Maintenance: The device enters an automated continuous operation state. The sensors are calibrated once a week, the expansion reactor is cleaned and disinfected, the activity of the microbial seed bank and the sealing of the pipeline are checked monthly, and the fault alarm unit monitors the operating status of each module in real time to ensure the long-term stable operation of the device.

[0081] IV. Implementation Results and Data Comparison The intelligent bio-generator in this embodiment operated continuously for 30 days to perform bio-enhanced treatment on the two-stage AO coking wastewater treatment system. The effluent water quality and bacterial activity were monitored on days 1, 7, 15, and 30 of operation. The treatment effect was compared with that of the original traditional bio-enhanced technology. The specific data are shown in Table 1 below: Table 1. Comparison of treatment effects between traditional bioaugmentation techniques and this embodiment. As can be seen from the above data, after the intelligent bio-generator of this embodiment has been running for 30 days, the effluent quality of the two-stage AO system is consistently better than the requirements of Table 2 of the "Emission Standard of Pollutants for Coking Chemical Industry" (GB 16171-2012). Specifically, COD is stable at 60mg / L-75mg / L, ammonia nitrogen is stable at 10mg / L-13mg / L, phenols are stable at 0.2mg / L-0.4mg / L, total cyanide is stable at 0.3mg / L-0.4mg / L, thiocyanate is stable at 6mg / L-8mg / L, and the bacterial activity is maintained at 93%-95%. Compared to traditional bioaugmentation technologies, the removal rate of pollutants is significantly improved. The total cyanide removal rate increases from 82.5%-87.5% to 99.0%-99.2%, the thiocyanate removal rate increases from 77.5%-85.0% to 99.0%-99.2%, and the bacterial activity increases by 43%-55%. Moreover, there are no problems such as bacterial imbalance or sudden drop in activity during operation, and the stability of the treatment effect is greatly optimized.

[0082] V. Implementation Conclusions This embodiment demonstrates that the intelligent bio-generator of the present invention can be precisely adapted to the two-stage AO process for coking wastewater. Targeting the characteristics of high cyanide, high thiocyanate, high phenol, and alkaline water quality, it completely solves the core pain points of traditional bioaugmentation technologies—insufficient bacterial activity, blind dosing, and lagging monitoring—through targeted matching of complex functional bacterial communities, on-site online cultivation to ensure high activity, dynamic and precise dosing, and closed-loop control. This device can effectively enhance the system's degradation efficiency and resistance to shock loads for recalcitrant pollutants, significantly improve the removal rate of characteristic pollutants such as total cyanide, thiocyanate, and phenols, maintain stable bacterial community structure and high activity, and ensure long-term compliance of effluent water quality standards.

[0083] Meanwhile, the intelligent bio-generator of this invention has a high degree of automation and reliable operation. It can be directly connected to the existing two-stage AO system without large-scale modification. The operating cost is reduced by more than 35% compared with traditional technology (mainly saving the cost of purchasing, storing and manually adding pre-made microbial agents). It has significant technical advantages, economic benefits and engineering application value, and can be widely promoted and applied to bio-enhanced treatment scenarios of coking and similar recalcitrant wastewater.

[0084] The above embodiments are only used to illustrate the technical ideas and features of the present invention, and are not intended to be unique or to limit the present invention. Those skilled in the art should understand that various changes or equivalent substitutions made to the present invention without departing from its scope are all within the scope of protection of the present invention.

Claims

1. An intelligent bio-source generator, characterized in that, include: The real-time monitoring module includes a characteristic pollutant sensor group, an online water quality monitor, a microbial activity monitoring device, and a data acquisition and transmission unit, which are used to detect the concentration changes of characteristic pollutants in the biochemical treatment system in real time; and to monitor key water quality parameters of wastewater. Used to monitor the activity status of indigenous and added microbial communities within the biochemical treatment system; used to collect monitoring data from various sensors and instruments and transmit it to the target identification and microbial community matching module and the dynamic addition and closed-loop control module; The targeted identification and microbial community matching module includes an industrial-grade main control chip, a microbial community database, a targeted matching algorithm and model unit, and a decision command output unit. These are used to respond to real-time monitoring data and complete targeted matching calculations, respectively; to set up a specific microbial community information database; to integrate big data analysis algorithms and microbial metabolic mechanism models; and to convert targeted matching results and amplification parameter commands into standardized control signals, which are then synchronously transmitted to the online amplification and activity assurance module and the dynamic dosing and closed-loop control module. The online propagation and activity assurance module includes a microbial seed bank, an online propagation reactor, an intelligent nutrient regulation unit, an environmental parameter regulation unit, and a propagation microbial activity detection unit. These are used, in turn, to store specific microbial seed liquid or seed powder; to propagate different functional microbial communities; to add the required nutrients according to the microbial community growth requirements output by the targeted identification and microbial community matching module; to regulate the propagation environmental parameters; and to monitor the activity and concentration changes of microorganisms in the online propagation reactor in real time. The dynamic dosing and closed-loop control module includes an automated dosing device, a flow metering unit, a closed-loop control controller, and a feedback adjustment unit, which are used to add specific bacterial groups to the corresponding biochemical tanks in sequence. Used for real-time monitoring of microbial inoculation flow rate and influent flow rate of biological treatment tank; used to generate control commands based on dynamic dosing control model and send them to automated dosing device; It is used to receive water quality monitoring data and bacterial activity data at the effluent of the biological treatment tank in real time, and compare them with preset discharge standards and bacterial activity thresholds. If the effluent water quality exceeds the standard or the bacterial activity is abnormal, it will automatically trigger the control command.

2. The intelligent bio-generator according to claim 1, characterized in that, The characteristic pollutant sensor group includes one or more of the following: polycyclic aromatic hydrocarbon sensors, nitrobenzene sensors, organophosphorus / chlorine sensors, antibiotic sensors, heterocyclic compound sensors, phenol sensors, total cyanide sensors, and thiocyanate sensors.

3. The intelligent bio-generator according to claim 1, characterized in that, Water quality online monitoring instruments include one or more of the following: COD online monitoring instruments, ammonia nitrogen online monitoring instruments, salinity monitoring instruments, pH monitoring instruments, dissolved oxygen monitoring instruments, temperature monitoring instruments, and toxicity monitoring instruments.

4. The intelligent bio-generator according to claim 1, characterized in that, The microbial community activity monitoring equipment uses a microbial respiration rate detector, which is simultaneously equipped with an ATP detector.

5. The intelligent bio-generator according to claim 1, characterized in that, The microbial community database includes one or more functional groups of bacteria, such as resistant bacteria, halophilic bacteria, highly efficient nitrifying bacteria, denitrifying bacteria, heavy metal reducing bacteria, polycyclic aromatic hydrocarbon degrading bacteria, organophosphorus degrading bacteria, azo dye degrading bacteria, thiocyanate degrading bacteria, phenol-resistant bacteria, and cyanide degrading bacteria, as well as the metabolic characteristics, suitable pollutant types, growth environment requirements, and community combination schemes of the above functional groups.

6. The intelligent bio-generator according to claim 1, characterized in that, Big data analysis algorithms and microbial metabolic mechanism models are used to output adaptive specific microbial communities and microbial community combination schemes, as well as the optimal growth conditions of the corresponding microbial communities, based on monitoring data of characteristic pollutant types, concentrations, water quality parameters, and microbial community activity.

7. The intelligent bio-generator according to claim 1, characterized in that, The intelligent nutrient regulation unit integrates a culture medium storage tank, a precision metering pump, and a nutrient ratio controller. The culture medium includes carbon sources, nitrogen sources, phosphorus sources, and trace elements.

8. The intelligent bio-generator according to claim 1, characterized in that, The environmental parameter control unit includes a temperature control device, a pH control device, and a dissolved oxygen control device.

9. The intelligent bio-generator according to claim 1, characterized in that, The culture bacteria activity detection unit has a built-in portable microbial activity detector.

10. The intelligent bio-generator according to claim 1, characterized in that, The closed-loop control controller adopts a PLC core controller and is equipped with a dynamic dosing control model. The dynamic dosing control model is used to automatically calculate the optimal dosing amount and dosing rate based on the data transmitted by the real-time monitoring module and the propagation progress, so as to realize the dynamic adaptation of dosing parameters.

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